Prupe.1G050100_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000286
Physical Location & Seq
Forward (+)
3511355 .. 3512288
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G050100.1

Sequence Viewer

Length: 636 bp
ATGGAAGAACTACATGCAGCAGCCTTAGCCTACTATTCCAATGGTTCTCCCGAACTTCAGCGTCTGGCATGGTCTTTCTTCCAATCCATGGACACAAACAACGATGGCCGAATCAGCTCCTCAGAGTTTTATGAATTTCTGCAGCAAAGCGGATACGGCTGGATTGTTAACGACCCCAGTTTCTTCACGAAACTAGATCGAAATGGCGACGGTGGCCTGGATTTCTACGAAGTGCTCACCTTCTACTACATCGTCAAAACGAGGAACATCTTGTGCCAAGGGTGTAGAGTGTACCTTTGTGGCTTGTATTTCACCTGCGTTGCTTGCTTTGATGTAGGAGCTGGCCATCATCAGGGCACCTATGATCTCTGTGCTGCCTGCTACGGCGTCAAGAATTTTTATCACCACCATACGTATTTCTTGGATAACCATGTCTTGCTGCGATCCAAAAGAGGCCTTCCTCCTTGTGCTAGCCCCGATCTCAATAAGGCAATGACGCCAGTTCCACAGCCTCTGGTGATTTATAACAATTACTACTTTCAAGCACCTGAAAGGAATAAATGGTTTCAAGCATATCGTCTATTTGAGATTGCTGTTGCTGCTGCAAGTATTGCTGCCAATTGTACAATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8 ZZ

Protein Analysis

212

Amino Acids

24.09

Weight (kDa)

6.13

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AarI CACCTGC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 1 cut(s) 150
AclWI GGATC 1 cut(s) 438
AcoI YGGCCR 2 cut(s) 106, 343
AcsI RAATTY 2 cut(s) 134, 394
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 2 cut(s) 387, 497
AfaI GTAC 2 cut(s) 293, 625
AfiI CCNNNNNNNGG 2 cut(s) 88, 352
AgsI TTSAA 2 cut(s) 542, 569
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 2 cut(s) 117, 341
AluI AGCT 2 cut(s) 117, 341
Alw21I GWGCWC 1 cut(s) 237
AlwI GGATC 1 cut(s) 438
AlwNI CAGNNNCTG 2 cut(s) 64, 514
AoxI GGCC 4 cut(s) 106, 214, 343, 454
ApeKI GCWGC 8 cut(s) 17, 20, 142, 374, 439, 599, 602, 614
ApoI RAATTY 2 cut(s) 134, 394
AsuHPI GGTGA 4 cut(s) 229, 304, 395, 529
AsuNHI GCTAGC 1 cut(s) 470
BaeGI GKGCMC 1 cut(s) 359
BalI TGGCCA 1 cut(s) 345
BanI GGYRCC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 8 cut(s) 29, 32, 154, 361, 426, 586, 589, 601
BccI CCATC 2 cut(s) 98, 354
BceAI ACGGC 2 cut(s) 172, 400
BciT130I CCWGG 1 cut(s) 218
BciVI GTATCC 1 cut(s) 146
BfaI CTAG 2 cut(s) 194, 471
BfmI CTRYAG 1 cut(s) 140
BfuAI ACCTGC 1 cut(s) 323
BfuI GTATCC 1 cut(s) 146
BisI GCNGC 8 cut(s) 18, 21, 143, 375, 440, 600, 603, 615
BlsI GCNGC 8 cut(s) 19, 22, 144, 376, 441, 601, 604, 616
Bme1390I CCNGG 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 1 cut(s) 218
BmrI ACTGGG 1 cut(s) 171
BmtI GCTAGC 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 25
BsaAI YACGTR 1 cut(s) 414
BsaHI GRCGYC 2 cut(s) 387, 497
BsaJI CCNNGG 2 cut(s) 87, 277
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 352
Bse1I ACTGG 2 cut(s) 177, 500
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 2 cut(s) 87, 277
BseLI CCNNNNNNNGG 2 cut(s) 88, 352
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 2 cut(s) 177, 500
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 359
BseXI GCAGC 8 cut(s) 29, 32, 154, 361, 426, 586, 589, 601
BshFI GGCC 4 cut(s) 108, 216, 345, 456
BshNI GGYRCC 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 88, 352
BsnI GGCC 4 cut(s) 108, 216, 345, 456
Bsp1286I GDGCHC 2 cut(s) 237, 359
Bsp1407I TGTACA 1 cut(s) 623
Bsp143I GATC 4 cut(s) 196, 364, 443, 478
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 1 cut(s) 150
BspANI GGCC 4 cut(s) 108, 216, 345, 456
BspCNI CTCAG 1 cut(s) 134
BspLI GGNNCC 1 cut(s) 358
BspMAI CTGCAG 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 323
BspOI GCTAGC 1 cut(s) 474
BspPI GGATC 1 cut(s) 438
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 498
BsrGI TGTACA 1 cut(s) 623
BsrI ACTGG 2 cut(s) 177, 500
BssECI CCNNGG 2 cut(s) 87, 277
BssMI GATC 4 cut(s) 196, 364, 443, 478
BssNI GRCGYC 2 cut(s) 387, 497
BssT1I CCWWGG 2 cut(s) 87, 277
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 212
BstACI GRCGYC 2 cut(s) 387, 497
BstAPI GCANNNNNTGC 1 cut(s) 611
BstAUI TGTACA 1 cut(s) 623
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 4 cut(s) 325, 343, 379, 472
BstDEI CTNAG 2 cut(s) 25, 121
BstDSI CCRYGG 1 cut(s) 87
BstKTI GATC 4 cut(s) 199, 367, 446, 481
BstMBI GATC 4 cut(s) 196, 364, 443, 478
BstMWI GCNNNNNNNGC 7 cut(s) 26, 114, 156, 213, 324, 599, 611
BstNI CCWGG 1 cut(s) 218
BstNSI RCATGY 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 216
BstSFI CTRYAG 1 cut(s) 140
BstSLI GKGCMC 1 cut(s) 359
BstSNI TACGTA 1 cut(s) 414
BstV1I GCAGC 8 cut(s) 29, 32, 154, 361, 426, 586, 589, 601
BsuI GTATCC 1 cut(s) 146
BsuRI GGCC 4 cut(s) 108, 216, 345, 456
BtgI CCRYGG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 4 cut(s) 325, 343, 379, 472
CaiI CAGNNNCTG 2 cut(s) 64, 514
CseI GACGC 3 cut(s) 50, 376, 505
Csp6I GTAC 2 cut(s) 292, 624
CviAII CATG 4 cut(s) 14, 69, 88, 431
CviQI GTAC 2 cut(s) 292, 624
DdeI CTNAG 2 cut(s) 25, 121
DpnI GATC 4 cut(s) 198, 366, 445, 480
DpnII GATC 4 cut(s) 196, 364, 443, 478
EaeI YGGCCR 2 cut(s) 106, 343
Eco105I TACGTA 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 87, 277
Eco147I AGGCCT 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 216
EcoT14I CCWWGG 2 cut(s) 87, 277
ErhI CCWWGG 2 cut(s) 87, 277
FaeI CATG 4 cut(s) 17, 72, 91, 434
FatI CATG 4 cut(s) 13, 68, 87, 430
Fnu4HI GCNGC 8 cut(s) 18, 21, 143, 375, 440, 600, 603, 615
Fsp4HI GCNGC 8 cut(s) 18, 21, 143, 375, 440, 600, 603, 615
FspBI CTAG 2 cut(s) 194, 471
GluI GCNGC 8 cut(s) 18, 21, 143, 375, 440, 600, 603, 615
HaeIII GGCC 4 cut(s) 108, 216, 345, 456
HgaI GACGC 3 cut(s) 50, 376, 505
Hin1I GRCGYC 2 cut(s) 387, 497
Hin1II CATG 4 cut(s) 17, 72, 91, 434
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HinfI GANTC 1 cut(s) 111
HpaI GTTAAC 1 cut(s) 169
HphI GGTGA 4 cut(s) 229, 304, 395, 529
Hpy166II GTNNAC 2 cut(s) 169, 292
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 3 cut(s) 50, 187, 391
Hpy8I GTNNAC 2 cut(s) 169, 292
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 2 cut(s) 250, 467
HpyCH4III ACNGT 1 cut(s) 212
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 3 cut(s) 17, 142, 605
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 114, 156, 213, 324, 599, 611
HpyF3I CTNAG 2 cut(s) 25, 121
HpySE526I ACGT 1 cut(s) 413
Hsp92I GRCGYC 2 cut(s) 387, 497
Hsp92II CATG 4 cut(s) 17, 72, 91, 434
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 4 cut(s) 196, 364, 443, 478
LmnI GCTCC 2 cut(s) 122, 338
Lsp1109I GCAGC 8 cut(s) 29, 32, 154, 361, 426, 586, 589, 601
MaeI CTAG 2 cut(s) 194, 471
MaeII ACGT 1 cut(s) 413
MalI GATC 4 cut(s) 198, 366, 445, 480
MboI GATC 4 cut(s) 196, 364, 443, 478
MboII GAAGA 3 cut(s) 17, 70, 175
MfeI CAATTG 1 cut(s) 619
MhlI GDGCHC 2 cut(s) 237, 359
MlsI TGGCCA 1 cut(s) 345
MluCI AATT 5 cut(s) 134, 394, 529, 619, 627
MluNI TGGCCA 1 cut(s) 345
MnlI CCTC 5 cut(s) 130, 255, 446, 471, 522
Mox20I TGGCCA 1 cut(s) 345
MscI TGGCCA 1 cut(s) 345
MseI TTAA 1 cut(s) 168
Msp20I TGGCCA 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 218
MunI CAATTG 1 cut(s) 619
MvaI CCWGG 1 cut(s) 218
MwoI GCNNNNNNNGC 7 cut(s) 26, 114, 156, 213, 324, 599, 611
NcoI CCATGG 1 cut(s) 87
NdeII GATC 4 cut(s) 196, 364, 443, 478
NheI GCTAGC 1 cut(s) 470
NlaIII CATG 4 cut(s) 17, 72, 91, 434
NlaIV GGNNCC 1 cut(s) 358
NspI RCATGY 1 cut(s) 17
PaqCI CACCTGC 1 cut(s) 323
PceI AGGCCT 1 cut(s) 456
PfeI GAWTC 1 cut(s) 111
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 8 cut(s) 19, 22, 144, 376, 441, 601, 604, 616
Ppu21I YACGTR 1 cut(s) 414
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 358
PstI CTGCAG 1 cut(s) 144
PstNI CAGNNNCTG 2 cut(s) 64, 514
RsaI GTAC 2 cut(s) 293, 625
RsaNI GTAC 2 cut(s) 292, 624
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 8 cut(s) 18, 21, 143, 375, 440, 600, 603, 615
Sau3AI GATC 4 cut(s) 196, 364, 443, 478
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 2 cut(s) 237, 359
SetI ASST 8 cut(s) 119, 242, 297, 317, 343, 362, 416, 550
SfcI CTRYAG 1 cut(s) 140
SnaBI TACGTA 1 cut(s) 414
Sse9I AATT 5 cut(s) 134, 394, 529, 619, 627
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 1 cut(s) 150
SspMI CTAG 2 cut(s) 194, 471
StuI AGGCCT 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 216
StyI CCWWGG 2 cut(s) 87, 277
TaaI ACNGT 1 cut(s) 212
TaiI ACGT 1 cut(s) 416
TaqI TCGA 1 cut(s) 199
TasI AATT 5 cut(s) 134, 394, 529, 619, 627
TatI WGTACW 1 cut(s) 623
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseI GCWGC 8 cut(s) 17, 20, 142, 374, 439, 599, 602, 614
TspDTI ATGAA 1 cut(s) 147
Van91I CCANNNNNTGG 1 cut(s) 88
XapI RAATTY 2 cut(s) 134, 394
XceI RCATGY 1 cut(s) 17
XspI CTAG 2 cut(s) 194, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.