Rw2G038350

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000286
Physical Location & Seq
Forward (+)
63305552 .. 63307526
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G038350.1

Sequence Viewer

Length: 906 bp
ATGGAATTGTTGTATAAAGGTTGCCATGGAGTATGTGATGGGAATGGATGTGAATCCCTTTTCGTCAAGGGTGGACTGTACTTTGTTTGCGTTGATTGCTTTGACGATCCAAACATTGAAAAAAGTTTCAATCTCTGTATCTCCTGCTACCGGTATGGAAAGTACATTCACCAGCATGAAAATTTTTTGGACAGCTTCATCTTGCTATGTTCCAAGTTTGTTCGTAAATCCACCGCACGGACCACAGTAACACAAAATCTTCAGATAGATCCAGAGATGCAAGAAATACGGTCTATGGCTCTCGCTTATTATCATAATCTGAAACCGGAGGACAAGCAGAAGGCAAATGAATTTTTTAGTGAAATGGATTCAGATGGTGACGGGGCAATAAGCCTCCAGGAATACGAGGAGAAATTTGGCAGCACCACCAGGATGAAGAAGTGTTTCGATGAGGTGGACATAAACGGAGATGGCAGGTTGGATTTTGAAGAAGTCATCACTCTTTTCTATCTTCACAAGGCTGGGAGGCTGGGAGTTTGGTGTAATGGCCGTGCATGTGTTCAAGCCCCTTTCCTCAAGGGACCTTACTTTGTTTGCGTCAACTGCTTTGATAGTAATAGGAAAGAAAGTACTTTCTACCTGTGCACCTCTTGCTACAGCACTGGAATGTACACTCACAGGCACAACAACTTTGTGGATAACTACATGTTGCTGCAAGACAAGTTTATGGCTCCTTACAAACCCGACCAAGGCCAAGCAGGAGCACAGAATCAACCAGCTACACAAAATTATCAGGACGCGTTGGTCGTGGCAAATCAATCATTCTCTATACTAGGCAACATTGTTAGCATTGGATTTAGTGTGGCGAGTTTTGCTATTAATTCCGCTGGGTGCAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

301

Amino Acids

34.06

Weight (kDa)

5.39

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 803
Acc36I ACCTGC 1 cut(s) 465
AccII CGCG 1 cut(s) 800
AciI CCGC 2 cut(s) 234, 885
AclWI GGATC 2 cut(s) 101, 263
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 3 cut(s) 181, 350, 413
AcuI CTGAAG 1 cut(s) 245
AfaI GTAC 4 cut(s) 80, 164, 631, 671
AfiI CCNNNNNNNGG 3 cut(s) 150, 237, 749
AflIII ACRYGT 2 cut(s) 705, 798
AgeI ACCGGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 119, 130, 488, 563
AjnI CCWGG 2 cut(s) 396, 428
AluBI AGCT 2 cut(s) 195, 779
AluI AGCT 2 cut(s) 195, 779
Alw21I GWGCWC 2 cut(s) 647, 766
Alw44I GTGCAC 1 cut(s) 643
AlwI GGATC 2 cut(s) 101, 263
AoxI GGCC 2 cut(s) 547, 751
ApaLI GTGCAC 1 cut(s) 643
ApeKI GCWGC 3 cut(s) 420, 712, 894
ApoI RAATTY 3 cut(s) 181, 350, 413
AseI ATTAAT 1 cut(s) 879
AsiGI ACCGGT 1 cut(s) 150
Asp700I GAANNNNTTC 1 cut(s) 443
AspS9I GGNCC 2 cut(s) 240, 581
AsuHPI GGTGA 2 cut(s) 161, 389
AvaII GGWCC 2 cut(s) 240, 581
BaeGI GKGCMC 1 cut(s) 647
Bbv12I GWGCWC 2 cut(s) 647, 766
BbvI GCAGC 2 cut(s) 432, 699
BccI CCATC 3 cut(s) 32, 368, 464
BceAI ACGGC 1 cut(s) 534
BciT130I CCWGG 2 cut(s) 398, 430
BfaI CTAG 1 cut(s) 833
BfmI CTRYAG 1 cut(s) 655
BfuAI ACCTGC 1 cut(s) 465
BisI GCNGC 3 cut(s) 421, 713, 895
BlsI GCNGC 3 cut(s) 422, 714, 896
BmcAI AGTACT 1 cut(s) 631
Bme1390I CCNGG 2 cut(s) 398, 430
Bme18I GGWCC 2 cut(s) 240, 581
BmgT120I GGNCC 2 cut(s) 240, 581
BmiI GGNNCC 2 cut(s) 582, 732
BmrFI CCNGG 2 cut(s) 398, 430
BmsI GCATC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 25, 748
BsaWI WCCGGW 2 cut(s) 150, 325
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 237, 749
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 1 cut(s) 667
Bse8I GATNNNNATC 1 cut(s) 52
BseBI CCWGG 2 cut(s) 398, 430
BseDI CCNNGG 2 cut(s) 25, 748
BseGI GGATG 2 cut(s) 53, 438
BseJI GATNNNNATC 1 cut(s) 52
BseLI CCNNNNNNNGG 3 cut(s) 150, 237, 749
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 1 cut(s) 422
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 2 cut(s) 432, 699
BseYI CCCAGC 3 cut(s) 521, 529, 887
Bsh1236I CGCG 1 cut(s) 800
BshFI GGCC 2 cut(s) 549, 753
BshTI ACCGGT 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 647, 766
BsiSI CCGG 2 cut(s) 151, 326
BslFI GGGAC 1 cut(s) 594
BslI CCNNNNNNNGG 3 cut(s) 150, 237, 749
BsmFI GGGAC 1 cut(s) 594
BsnI GGCC 2 cut(s) 549, 753
Bsp1286I GDGCHC 2 cut(s) 647, 766
Bsp1407I TGTACA 1 cut(s) 669
Bsp143I GATC 2 cut(s) 106, 268
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 2 cut(s) 234, 885
BspANI GGCC 2 cut(s) 549, 753
BspFNI CGCG 1 cut(s) 800
BspLI GGNNCC 2 cut(s) 582, 732
BspMI ACCTGC 1 cut(s) 465
BspPI GGATC 2 cut(s) 101, 263
BsrFI RCCGGY 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 669
BsrI ACTGG 1 cut(s) 667
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 25, 748
BssMI GATC 2 cut(s) 106, 268
BssT1I CCWWGG 2 cut(s) 25, 748
Bst2UI CCWGG 2 cut(s) 398, 430
Bst4CI ACNGT 3 cut(s) 78, 247, 291
BstAPI GCANNNNNTGC 1 cut(s) 651
BstAUI TGTACA 1 cut(s) 669
BstDSI CCRYGG 1 cut(s) 25
BstF5I GGATG 2 cut(s) 53, 438
BstFNI CGCG 1 cut(s) 800
BstKTI GATC 2 cut(s) 109, 271
BstMBI GATC 2 cut(s) 106, 268
BstMWI GCNNNNNNNGC 4 cut(s) 96, 603, 651, 872
BstNI CCWGG 2 cut(s) 398, 430
BstNSI RCATGY 2 cut(s) 558, 709
BstSCI CCNGG 2 cut(s) 396, 428
BstSFI CTRYAG 1 cut(s) 655
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 1 cut(s) 800
BstV1I GCAGC 2 cut(s) 432, 699
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 2 cut(s) 549, 753
BtgI CCRYGG 1 cut(s) 25
BtsCI GGATG 2 cut(s) 53, 438
BtsIMutI CAGTG 1 cut(s) 660
BveI ACCTGC 1 cut(s) 465
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 240, 581
CseI GACGC 2 cut(s) 586, 806
Csp6I GTAC 4 cut(s) 79, 163, 630, 670
CspAI ACCGGT 1 cut(s) 150
CviAII CATG 4 cut(s) 26, 176, 555, 706
CviQI GTAC 4 cut(s) 79, 163, 630, 670
DpnI GATC 2 cut(s) 108, 270
DpnII GATC 2 cut(s) 106, 268
DrdI GACNNNNNNGTC 1 cut(s) 803
DseDI GACNNNNNNGTC 1 cut(s) 803
EaeI YGGCCR 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 25, 748
Eco47I GGWCC 2 cut(s) 240, 581
Eco57I CTGAAG 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 396, 428
EcoT14I CCWWGG 2 cut(s) 25, 748
ErhI CCWWGG 2 cut(s) 25, 748
FaeI CATG 4 cut(s) 29, 179, 558, 709
FaqI GGGAC 1 cut(s) 594
FatI CATG 4 cut(s) 25, 175, 554, 705
Fnu4HI GCNGC 3 cut(s) 421, 713, 895
FokI GGATG 2 cut(s) 60, 445
Fsp4HI GCNGC 3 cut(s) 421, 713, 895
FspBI CTAG 1 cut(s) 833
GluI GCNGC 3 cut(s) 421, 713, 895
GsaI CCCAGC 3 cut(s) 525, 533, 891
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 2 cut(s) 549, 753
HapII CCGG 2 cut(s) 151, 326
HgaI GACGC 2 cut(s) 586, 806
Hin1II CATG 4 cut(s) 29, 179, 558, 709
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 3 cut(s) 53, 368, 769
HpaII CCGG 2 cut(s) 151, 326
HphI GGTGA 2 cut(s) 161, 389
Hpy166II GTNNAC 5 cut(s) 74, 457, 601, 645, 672
Hpy188I TCNGA 3 cut(s) 264, 321, 373
Hpy188III TCNNGA 2 cut(s) 272, 794
Hpy8I GTNNAC 5 cut(s) 74, 457, 601, 645, 672
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 3 cut(s) 78, 247, 291
HpyCH4V TGCA 5 cut(s) 280, 554, 645, 715, 894
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 603, 651, 872
Hsp92II CATG 4 cut(s) 29, 179, 558, 709
Kzo9I GATC 2 cut(s) 106, 268
LmnI GCTCC 2 cut(s) 736, 761
Lsp1109I GCAGC 2 cut(s) 432, 699
LweI GCATC 1 cut(s) 267
MaeI CTAG 1 cut(s) 833
MaeIII GTNAC 2 cut(s) 247, 377
MalI GATC 2 cut(s) 108, 270
MboI GATC 2 cut(s) 106, 268
MboII GAAGA 4 cut(s) 251, 448, 500, 503
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 2 cut(s) 647, 766
MluCI AATT 6 cut(s) 5, 181, 350, 413, 787, 880
MluI ACGCGT 1 cut(s) 798
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 7 cut(s) 322, 400, 404, 445, 519, 584, 658
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 1 cut(s) 879
MslI CAYNNNNRTG 3 cut(s) 174, 431, 665
MspA1I CMGCKG 1 cut(s) 887
MspI CCGG 2 cut(s) 151, 326
MspR9I CCNGG 2 cut(s) 398, 430
MvaI CCWGG 2 cut(s) 398, 430
MvnI CGCG 1 cut(s) 800
MwoI GCNNNNNNNGC 4 cut(s) 96, 603, 651, 872
NcoI CCATGG 1 cut(s) 25
NdeII GATC 2 cut(s) 106, 268
NlaIII CATG 4 cut(s) 29, 179, 558, 709
NlaIV GGNNCC 2 cut(s) 582, 732
NmuCI GTSAC 1 cut(s) 377
NspI RCATGY 2 cut(s) 558, 709
PciI ACATGT 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 3 cut(s) 53, 368, 769
PfoI TCCNGGA 1 cut(s) 396
PinAI ACCGGT 1 cut(s) 150
PkrI GCNGC 3 cut(s) 422, 714, 896
PpuMI RGGWCCY 1 cut(s) 581
PscI ACATGT 1 cut(s) 705
PshBI ATTAAT 1 cut(s) 879
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 396, 428
PspFI CCCAGC 3 cut(s) 521, 529, 887
PspGI CCWGG 2 cut(s) 396, 428
PspN4I GGNNCC 2 cut(s) 582, 732
PspPI GGNCC 2 cut(s) 240, 581
PspPPI RGGWCCY 1 cut(s) 581
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 4 cut(s) 80, 164, 631, 671
RsaNI GTAC 4 cut(s) 79, 163, 630, 670
RseI CAYNNNNRTG 3 cut(s) 174, 431, 665
SaqAI TTAA 1 cut(s) 879
SatI GCNGC 3 cut(s) 421, 713, 895
Sau3AI GATC 2 cut(s) 106, 268
Sau96I GGNCC 2 cut(s) 240, 581
ScaI AGTACT 1 cut(s) 631
ScrFI CCNGG 2 cut(s) 398, 430
SduI GDGCHC 2 cut(s) 647, 766
SetI ASST 8 cut(s) 22, 197, 456, 479, 586, 642, 650, 781
SfaNI GCATC 1 cut(s) 267
SfcI CTRYAG 1 cut(s) 655
SinI GGWCC 2 cut(s) 240, 581
SmiMI CAYNNNNRTG 3 cut(s) 174, 431, 665
SmlI CTYRAG 1 cut(s) 575
SmoI CTYRAG 1 cut(s) 575
Sse9I AATT 6 cut(s) 5, 181, 350, 413, 787, 880
SsiI CCGC 2 cut(s) 234, 885
SspMI CTAG 1 cut(s) 833
StyD4I CCNGG 2 cut(s) 396, 428
StyI CCWWGG 2 cut(s) 25, 748
TaaI ACNGT 3 cut(s) 78, 247, 291
TaqI TCGA 1 cut(s) 447
TasI AATT 6 cut(s) 5, 181, 350, 413, 787, 880
TatI WGTACW 4 cut(s) 78, 162, 629, 669
TfiI GAWTC 3 cut(s) 53, 368, 769
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 1 cut(s) 667
TseFI GTSAC 1 cut(s) 377
TseI GCWGC 3 cut(s) 420, 712, 894
Tsp45I GTSAC 1 cut(s) 377
TspDTI ATGAA 4 cut(s) 187, 192, 363, 449
TspGWI ACGGA 2 cut(s) 253, 480
TspRI CASTG 1 cut(s) 667
VneI GTGCAC 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 240, 581
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 3 cut(s) 181, 350, 413
XceI RCATGY 2 cut(s) 558, 709
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 1 cut(s) 833
ZrmI AGTACT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.