Rroxscaffold_1G00054620

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000286
Physical Location & Seq
Forward (+)
75992942 .. 75994016
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054620.1

Sequence Viewer

Length: 636 bp
ATGGACGAGTTGCATCAAGCAGCTTCTGCATATTACAACAACGGGTCACCTGAGATTCAGCAACTCGCATGGAGCTTCTTCCAATCCATGGACACAAACTGCGACAGCCAAGTTGACTGGAACGAGTTCTCCGCTGACTCCGCATTTCTCTACCAAAGCAGCAACCAATGGATCAGCCCCAGCTTCTTCAACGATCTGGACCGGAACCACGATGGAGCCCTTGATTTCATGGAAGTTCTCACGTTTTACTACATTATCAAGACCAGGAGCTTCTGGTGCAGTGTTTGCGGGATATGTCTCACGGGGCTTTACTTCACTTATGTTGAGTGCTTTGACAACGTGAACTCAAACACTTATGATCTTTGTCCCAGATGCTATGAGTCAAAGCCGGAAAGGCAAAGGTATAACCAGCAGAGTGATCACCATCATGCCTACTTTATGGATAACCATATGTTGCTCAGATCAAAGAGAGGAGGATATATGGTTCTGCAAACCTGTTTCAAGAATATTGACAAAATGCATGATATGCAGGCCATAGATTCCCCTGGAGGTGGACCAGGACGGACCAGAATGCGCCAAACGATGAAAGCATTGGATACAGCAGTGAATGTTGCAACTTTGTGTAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8 ZZ

Protein Analysis

211

Amino Acids

24.48

Weight (kDa)

5.23

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 3 cut(s) 132, 141, 288
AclWI GGATC 1 cut(s) 179
AfiI CCNNNNNNNGG 2 cut(s) 88, 551
AgsI TTSAA 2 cut(s) 190, 502
AjnI CCWGG 3 cut(s) 263, 544, 556
AloI GAACNNNNNNTCC 4 cut(s) 113, 145, 468, 500
AluBI AGCT 4 cut(s) 23, 75, 183, 270
AluI AGCT 4 cut(s) 23, 75, 183, 270
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 1 cut(s) 179
AlwNI CAGNNNCTG 1 cut(s) 26
AoxI GGCC 1 cut(s) 531
ApeKI GCWGC 2 cut(s) 20, 159
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 3 cut(s) 199, 554, 564
AsuHPI GGTGA 2 cut(s) 39, 413
AvaII GGWCC 3 cut(s) 199, 554, 564
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BanII GRGCYC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 32, 171
BccI CCATC 2 cut(s) 206, 432
BciT130I CCWGG 3 cut(s) 265, 546, 558
BciVI GTATCC 1 cut(s) 589
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 302
BfuI GTATCC 1 cut(s) 589
BglI GCCNNNNNGGC 1 cut(s) 394
BisI GCNGC 2 cut(s) 21, 160
BlsI GCNGC 2 cut(s) 22, 161
Bme1390I CCNGG 3 cut(s) 265, 546, 558
Bme18I GGWCC 3 cut(s) 199, 554, 564
BmgT120I GGNCC 3 cut(s) 199, 554, 564
BmiI GGNNCC 2 cut(s) 206, 217
BmrFI CCNGG 3 cut(s) 265, 546, 558
BmsI GCATC 2 cut(s) 22, 362
BpmI CTGGAG 1 cut(s) 567
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 87, 544
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 551
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 423
BseBI CCWGG 3 cut(s) 265, 546, 558
BseDI CCNNGG 2 cut(s) 87, 544
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 2 cut(s) 88, 551
BseMII CTCAG 2 cut(s) 42, 472
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 2 cut(s) 32, 171
BseYI CCCAGC 1 cut(s) 179
BsgI GTGCAG 1 cut(s) 298
BshFI GGCC 1 cut(s) 533
BsiSI CCGG 2 cut(s) 202, 389
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 2 cut(s) 88, 551
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 351
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 5 cut(s) 171, 193, 358, 418, 461
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 3 cut(s) 132, 141, 288
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 43, 471
BspLI GGNNCC 2 cut(s) 206, 217
BspPI GGATC 1 cut(s) 179
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 87, 544
BssMI GATC 5 cut(s) 171, 193, 358, 418, 461
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 265, 546, 558
BstAPI GCANNNNNTGC 3 cut(s) 26, 285, 526
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 2 cut(s) 51, 458
BstDSI CCRYGG 1 cut(s) 87
BstEII GGTNACC 1 cut(s) 45
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 5 cut(s) 174, 196, 361, 421, 464
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 5 cut(s) 171, 193, 358, 418, 461
BstMWI GCNNNNNNNGC 6 cut(s) 26, 140, 276, 285, 394, 526
BstNI CCWGG 3 cut(s) 265, 546, 558
BstPI GGTNACC 1 cut(s) 45
BstSCI CCNGG 3 cut(s) 263, 544, 556
BstV1I GCAGC 2 cut(s) 32, 171
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 1 cut(s) 533
BtgI CCRYGG 1 cut(s) 87
BtsI GCAGTG 2 cut(s) 286, 609
BtsIMutI CAGTG 2 cut(s) 286, 609
Cac8I GCNNGC 1 cut(s) 531
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 1 cut(s) 576
Cfr13I GGNCC 3 cut(s) 199, 554, 564
CviAII CATG 5 cut(s) 69, 88, 229, 428, 521
DdeI CTNAG 2 cut(s) 51, 458
DpnI GATC 5 cut(s) 173, 195, 360, 420, 463
DpnII GATC 5 cut(s) 171, 193, 358, 418, 461
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 220
Eco47I GGWCC 3 cut(s) 199, 554, 564
Eco91I GGTNACC 1 cut(s) 45
EcoO65I GGTNACC 1 cut(s) 45
EcoRII CCWGG 3 cut(s) 263, 544, 556
EcoT14I CCWWGG 1 cut(s) 87
EcoT22I ATGCAT 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 72, 91, 232, 431, 524
FaqI GGGAC 1 cut(s) 351
FatI CATG 5 cut(s) 68, 87, 228, 427, 520
FauI CCCGC 1 cut(s) 281
FauNDI CATATG 1 cut(s) 450
FbaI TGATCA 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 21, 160
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 2 cut(s) 21, 160
GlaI GCGC 1 cut(s) 575
GluI GCNGC 2 cut(s) 21, 160
GsaI CCCAGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 1 cut(s) 533
HapII CCGG 2 cut(s) 202, 389
HhaI GCGC 1 cut(s) 576
Hin1II CATG 5 cut(s) 72, 91, 232, 431, 524
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 4 cut(s) 55, 137, 380, 539
HpaII CCGG 2 cut(s) 202, 389
HphI GGTGA 2 cut(s) 39, 413
Hpy166II GTNNAC 3 cut(s) 115, 343, 554
Hpy188I TCNGA 1 cut(s) 461
Hpy188III TCNNGA 3 cut(s) 197, 259, 502
Hpy8I GTNNAC 3 cut(s) 115, 343, 554
HpyCH4IV ACGT 2 cut(s) 242, 339
HpyCH4V TGCA 7 cut(s) 13, 29, 279, 490, 520, 529, 614
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 140, 276, 285, 394, 526
HpyF3I CTNAG 2 cut(s) 51, 458
HpySE526I ACGT 2 cut(s) 242, 339
Hsp92II CATG 5 cut(s) 72, 91, 232, 431, 524
HspAI GCGC 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 5 cut(s) 171, 193, 358, 418, 461
LmnI GCTCC 3 cut(s) 72, 215, 267
Lsp1109I GCAGC 2 cut(s) 32, 171
LweI GCATC 2 cut(s) 22, 362
MaeII ACGT 2 cut(s) 242, 339
MaeIII GTNAC 1 cut(s) 45
MalI GATC 5 cut(s) 173, 195, 360, 420, 463
MboI GATC 5 cut(s) 171, 193, 358, 418, 461
MboII GAAGA 2 cut(s) 70, 178
MhlI GDGCHC 1 cut(s) 220
MlyI GAGTC 2 cut(s) 131, 389
MnlI CCTC 3 cut(s) 464, 467, 542
Mph1103I ATGCAT 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 125
MslI CAYNNNNRTG 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 2 cut(s) 202, 389
MspR9I CCNGG 3 cut(s) 265, 546, 558
Mva1269I GAATGC 1 cut(s) 576
MvaI CCWGG 3 cut(s) 265, 546, 558
MwoI GCNNNNNNNGC 6 cut(s) 26, 140, 276, 285, 394, 526
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 450
NdeII GATC 5 cut(s) 171, 193, 358, 418, 461
NlaIII CATG 5 cut(s) 72, 91, 232, 431, 524
NlaIV GGNNCC 2 cut(s) 206, 217
NmuCI GTSAC 1 cut(s) 45
NsiI ATGCAT 1 cut(s) 522
PctI GAATGC 1 cut(s) 576
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 55, 539
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 2 cut(s) 22, 161
PleI GAGTC 2 cut(s) 131, 388
PpsI GAGTC 2 cut(s) 131, 388
Psp6I CCWGG 3 cut(s) 263, 544, 556
PspEI GGTNACC 1 cut(s) 45
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 3 cut(s) 263, 544, 556
PspN4I GGNNCC 2 cut(s) 206, 217
PspPI GGNCC 3 cut(s) 199, 554, 564
PstNI CAGNNNCTG 1 cut(s) 26
RseI CAYNNNNRTG 1 cut(s) 426
SatI GCNGC 2 cut(s) 21, 160
Sau3AI GATC 5 cut(s) 171, 193, 358, 418, 461
Sau96I GGNCC 3 cut(s) 199, 554, 564
SchI GAGTC 2 cut(s) 131, 389
ScrFI CCNGG 3 cut(s) 265, 546, 558
SduI GDGCHC 1 cut(s) 220
SfaNI GCATC 2 cut(s) 22, 362
SinI GGWCC 3 cut(s) 199, 554, 564
SmiMI CAYNNNNRTG 1 cut(s) 426
SsiI CCGC 3 cut(s) 132, 141, 288
SspI AATATT 1 cut(s) 508
StyD4I CCNGG 3 cut(s) 263, 544, 556
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 2 cut(s) 245, 342
TfiI GAWTC 2 cut(s) 55, 539
TscAI CASTG 2 cut(s) 286, 609
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 2 cut(s) 20, 159
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 2 cut(s) 217, 599
TspGWI ACGGA 1 cut(s) 577
TspRI CASTG 2 cut(s) 286, 609
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 199, 554, 564
XmnI GAANNNNTTC 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.