Rroxscaffold_1G00054630

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000286
Physical Location & Seq
Forward (+)
76013786 .. 76016481
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054630.1

Sequence Viewer

Length: 576 bp
ATGGAGGAATTACACCAGGCCGCTATAGCATACTACAACAACGGGTCACAGAATCTCCAGGTACTAGCCTCGAACTTCTTCGAAGCCCTGGACACTAACGGTGATGGTGAAGTCAGCTGGTCAGAGTTTGTCACATTTCTCCAGCAGAGCGGGTACAATATCGACCCCGACGTATTCAACGAGCTCGATCAAGACGGCGATGGTTACCTCGATTTCATGGAAGTTCTCACACTGTACTACATCATCAAGACCAGGTTCTTTGCTTGTGATGACTGCGGAGTACGCCTCTTGGGGCTTTATTTCACGTGTGTCGAGTGCTTCGACAGAGCCACCGATACCTTTGATCTGTGTTCCATCTGCTATGCTGCAAAGCAGTTCCGTCACCACCATACTCTCTTCTTGGATAATCATATGCTGCTCATAGCAAAGAGAAGGCAGGCTCTTGGTAGTCATAACCTGAATCAGGCAATAGTTCCGGCACAACAGCAGCCTCCAACTACACCTGCAAGGGAGAAGAAGTTGACTTTCCCTATATGGTCTAGGAGCAGTCATGCCTCTCCATTTATAAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

191

Amino Acids

21.82

Weight (kDa)

5.1

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_5 PF13202 26 - 47 5e-06 EF hand
EF-hand_7 PF13499 26 - 80 4.7e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AarI CACCTGC 1 cut(s) 511
Acc36I ACCTGC 1 cut(s) 511
AccBSI CCGCTC 1 cut(s) 150
AciI CCGC 3 cut(s) 21, 150, 276
AcvI CACGTG 1 cut(s) 306
AfaI GTAC 4 cut(s) 63, 155, 236, 282
AfiI CCNNNNNNNGG 1 cut(s) 463
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 1 cut(s) 178
AjnI CCWGG 4 cut(s) 15, 57, 87, 251
AluBI AGCT 2 cut(s) 117, 184
AluI AGCT 2 cut(s) 117, 184
Alw21I GWGCWC 1 cut(s) 186
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 3 cut(s) 365, 415, 487
Asp700I GAANNNNTTC 1 cut(s) 77
AsuHPI GGTGA 3 cut(s) 113, 119, 374
AsuII TTCGAA 1 cut(s) 81
BanII GRGCYC 1 cut(s) 186
BbrPI CACGTG 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 3 cut(s) 352, 402, 499
BccI CCATC 3 cut(s) 98, 194, 362
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 4 cut(s) 17, 59, 89, 253
BfaI CTAG 2 cut(s) 65, 540
BfmI CTRYAG 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 511
BisI GCNGC 4 cut(s) 21, 366, 416, 488
BlsI GCNGC 4 cut(s) 22, 367, 417, 489
Bme1390I CCNGG 4 cut(s) 17, 59, 89, 253
BmrFI CCNGG 4 cut(s) 17, 59, 89, 253
BplI GAGNNNNNCTC 2 cut(s) 270, 302
BpmI CTGGAG 2 cut(s) 41, 125
Bpu14I TTCGAA 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 503, 533
Bsc4I CCNNNNNNNGG 1 cut(s) 463
BseBI CCWGG 4 cut(s) 17, 59, 89, 253
BseDI CCNNGG 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 463
BseXI GCAGC 3 cut(s) 352, 402, 499
BshFI GGCC 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 476
BslI CCNNNNNNNGG 1 cut(s) 463
BsnI GGCC 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 187, 343
BspACI CCGC 3 cut(s) 21, 150, 276
BspANI GGCC 1 cut(s) 20
BspMI ACCTGC 1 cut(s) 511
BspT104I TTCGAA 1 cut(s) 81
BsrBI CCGCTC 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 2 cut(s) 187, 343
Bst2UI CCWGG 4 cut(s) 17, 59, 89, 253
Bst4CI ACNGT 2 cut(s) 101, 234
Bst6I CTCTTC 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 438
BstEII GGTNACC 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 461
BstKTI GATC 2 cut(s) 190, 346
BstMBI GATC 2 cut(s) 187, 343
BstMWI GCNNNNNNNGC 2 cut(s) 26, 282
BstNI CCWGG 4 cut(s) 17, 59, 89, 253
BstPI GGTNACC 1 cut(s) 203
BstSCI CCNGG 4 cut(s) 15, 57, 87, 251
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 352, 402, 499
BsuRI GGCC 1 cut(s) 20
BtgZI GCGATG 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 230
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 1 cut(s) 438
CsiI ACCWGGT 1 cut(s) 251
Csp6I GTAC 4 cut(s) 62, 154, 235, 281
CviAII CATG 2 cut(s) 217, 551
CviQI GTAC 4 cut(s) 62, 154, 235, 281
DpnI GATC 2 cut(s) 189, 345
DpnII GATC 2 cut(s) 187, 343
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 184
Eco72I CACGTG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO65I GGTNACC 1 cut(s) 203
EcoRII CCWGG 4 cut(s) 15, 57, 87, 251
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 2 cut(s) 220, 554
FatI CATG 2 cut(s) 216, 550
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 21, 366, 416, 488
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 4 cut(s) 21, 366, 416, 488
FspBI CTAG 2 cut(s) 65, 540
GluI GCNGC 4 cut(s) 21, 366, 416, 488
GsuI CTGGAG 2 cut(s) 41, 125
HaeIII GGCC 1 cut(s) 20
HapII CCGG 1 cut(s) 476
Hin1II CATG 2 cut(s) 220, 554
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HinfI GANTC 2 cut(s) 52, 460
HpaII CCGG 1 cut(s) 476
HphI GGTGA 3 cut(s) 113, 119, 374
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 2 cut(s) 191, 247
Hpy8I GTNNAC 1 cut(s) 522
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 426
HpyCH4III ACNGT 2 cut(s) 101, 234
HpyCH4IV ACGT 2 cut(s) 171, 305
HpyCH4V TGCA 2 cut(s) 368, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 282
HpySE526I ACGT 2 cut(s) 171, 305
Hsp92II CATG 2 cut(s) 220, 554
Kzo9I GATC 2 cut(s) 187, 343
LmnI GCTCC 1 cut(s) 543
Lsp1109I GCAGC 3 cut(s) 352, 402, 499
MabI ACCWGGT 1 cut(s) 251
MaeI CTAG 2 cut(s) 65, 540
MaeII ACGT 2 cut(s) 171, 305
MaeIII GTNAC 4 cut(s) 45, 130, 203, 380
MalI GATC 2 cut(s) 189, 345
MbiI CCGCTC 1 cut(s) 150
MboI GATC 2 cut(s) 187, 343
MboII GAAGA 3 cut(s) 70, 388, 526
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 1 cut(s) 8
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 5 cut(s) 79, 218, 296, 501, 565
MroXI GAANNNNTTC 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 4 cut(s) 17, 59, 89, 253
MvaI CCWGG 4 cut(s) 17, 59, 89, 253
MwoI GCNNNNNNNGC 2 cut(s) 26, 282
NdeI CATATG 1 cut(s) 411
NdeII GATC 2 cut(s) 187, 343
NlaIII CATG 2 cut(s) 220, 554
NmuCI GTSAC 3 cut(s) 45, 130, 380
NspV TTCGAA 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 511
PcsI WCGNNNNNNNCGW 3 cut(s) 168, 177, 318
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 2 cut(s) 52, 460
PkrI GCNGC 4 cut(s) 22, 367, 417, 489
PmaCI CACGTG 1 cut(s) 306
PmlI CACGTG 1 cut(s) 306
Ppu21I YACGTR 1 cut(s) 306
PsiI TTATAA 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 4 cut(s) 15, 57, 87, 251
PspCI CACGTG 1 cut(s) 306
PspEI GGTNACC 1 cut(s) 203
PspGI CCWGG 4 cut(s) 15, 57, 87, 251
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 4 cut(s) 63, 155, 236, 282
RsaNI GTAC 4 cut(s) 62, 154, 235, 281
SacI GAGCTC 1 cut(s) 186
SatI GCNGC 4 cut(s) 21, 366, 416, 488
Sau3AI GATC 2 cut(s) 187, 343
ScrFI CCNGG 4 cut(s) 17, 59, 89, 253
SduI GDGCHC 1 cut(s) 186
SexAI ACCWGGT 1 cut(s) 251
SfcI CTRYAG 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 81
Sse9I AATT 1 cut(s) 8
SsiI CCGC 3 cut(s) 21, 150, 276
SspMI CTAG 2 cut(s) 65, 540
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 4 cut(s) 15, 57, 87, 251
TaaI ACNGT 2 cut(s) 101, 234
TaiI ACGT 2 cut(s) 174, 308
TaqI TCGA 7 cut(s) 71, 81, 162, 186, 210, 312, 321
TasI AATT 1 cut(s) 8
TatI WGTACW 1 cut(s) 234
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 2 cut(s) 52, 460
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 3 cut(s) 45, 130, 380
TseI GCWGC 3 cut(s) 365, 415, 487
Tsp45I GTSAC 3 cut(s) 45, 130, 380
TspDTI ATGAA 1 cut(s) 205
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 1 cut(s) 237
XagI CCTNNNNNAGG 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 2 cut(s) 65, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.