FvH4_3g15020

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000286
Physical Location & Seq
Reverse (-)
9306070 .. 9307897
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15020.t1

Sequence Viewer

Length: 642 bp
ATGGAGGAATTAAACCAGGCTGCTATAGCATACTACAATAATGGGTCACAGAATCTCCAGACACTAGCCTTGGATTTCTTCCAATCCTTGGACACCAACGGTGACGGTGGAGTCAGCTGGTCGGAGTTCATCTCGTTTCTCCAGCAAAGCGGCTACGACATTGACCCTGACATGTTCAACGTGCTCGATGAAGACGGTGATGGTTACCTAGACTTCAGGGAAGTTCTCACACTGTACTACATCATCAAGACCAGGTTCTTTTCTTGTGTTGGCTGTGGAGAACGCCTCTTAGGGTTGTACTTCACGTGTGTCGAGTGCTTCGACAGAGCCAACGATACCTTTGATCTGTGCTCCAACTGCTATGCTGAAAAGCAGTTCCGTCACCGCCATACACTCTTCTTGGATAATCATATGCTGCTTATAGCAAAGAGACGACAGGCTCTTGGTAATCATAACCTGAGTCAGGCAATAGGTCGACAAACTTCATATCAAGCTCCACCTACATCTGCAAGCAGGGCAATAGTTCCAGCACGACAGCAGCCTCCAGCTACACCTGCAAGGAACAGATTGCGTTCAACATTTCGTGCATTAGAAACCGCAGTTGGAGTTGCAAATTTGATGGCTTCGTGTACCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8

Protein Analysis

214

Amino Acids

24.03

Weight (kDa)

5.33

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 25 - 80 3.3e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 562
AasI GACNNNNNNGTC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 562
AccB7I CCANNNNNTGG 1 cut(s) 88
AccI GTMKAC 1 cut(s) 475
AciI CCGC 3 cut(s) 150, 385, 597
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 1 cut(s) 199
AcvI CACGTG 1 cut(s) 306
AfaI GTAC 3 cut(s) 236, 299, 631
AfiI CCNNNNNNNGG 2 cut(s) 88, 463
AflIII ACRYGT 2 cut(s) 171, 305
AgsI TTSAA 2 cut(s) 178, 576
AjnI CCWGG 2 cut(s) 15, 251
AjuI GAANNNNNNNTTGG 2 cut(s) 585, 617
AluBI AGCT 3 cut(s) 117, 494, 548
AluI AGCT 3 cut(s) 117, 494, 548
Alw21I GWGCWC 2 cut(s) 186, 353
Alw26I GTCTC 1 cut(s) 424
ApeKI GCWGC 3 cut(s) 20, 415, 538
ApoI RAATTY 1 cut(s) 613
AsuHPI GGTGA 3 cut(s) 113, 209, 374
BbrPI CACGTG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 186, 353
BbvI GCAGC 3 cut(s) 7, 402, 550
BccI CCATC 2 cut(s) 194, 613
BciT130I CCWGG 2 cut(s) 17, 253
BcoDI GTCTC 1 cut(s) 424
BfaI CTAG 2 cut(s) 65, 209
BfmI CTRYAG 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 562
BisI GCNGC 4 cut(s) 21, 151, 416, 539
BlsI GCNGC 4 cut(s) 22, 152, 417, 540
Bme1390I CCNGG 2 cut(s) 17, 253
BmrFI CCNGG 2 cut(s) 17, 253
BpiI GAAGAC 1 cut(s) 198
BplI GAGNNNNNCTC 4 cut(s) 116, 148, 270, 302
BpmI CTGGAG 3 cut(s) 41, 125, 528
BsaAI YACGTR 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 69, 87
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 463
BseBI CCWGG 2 cut(s) 17, 253
BseDI CCNNGG 2 cut(s) 69, 87
BseLI CCNNNNNNNGG 2 cut(s) 88, 463
BseMII CTCAG 1 cut(s) 449
BseXI GCAGC 3 cut(s) 7, 402, 550
BsiHKAI GWGCWC 2 cut(s) 186, 353
BslI CCNNNNNNNGG 2 cut(s) 88, 463
BsmAI GTCTC 1 cut(s) 424
BsmBI CGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 186, 353
Bsp143I GATC 1 cut(s) 343
BspACI CCGC 3 cut(s) 150, 385, 597
BspCNI CTCAG 1 cut(s) 450
BspMI ACCTGC 1 cut(s) 562
BssECI CCNNGG 2 cut(s) 69, 87
BssMI GATC 1 cut(s) 343
BssT1I CCWWGG 2 cut(s) 69, 87
Bst2UI CCWGG 2 cut(s) 17, 253
Bst4CI ACNGT 4 cut(s) 101, 107, 197, 234
Bst6I CTCTTC 1 cut(s) 401
BstBAI YACGTR 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 2 cut(s) 289, 458
BstEII GGTNACC 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 461
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 4 cut(s) 26, 357, 515, 554
BstNI CCWGG 2 cut(s) 17, 253
BstNSI RCATGY 1 cut(s) 175
BstPI GGTNACC 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 15, 251
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 3 cut(s) 7, 402, 550
BstV2I GAAGAC 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 230
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 511
CsiI ACCWGGT 1 cut(s) 251
Csp6I GTAC 3 cut(s) 235, 298, 630
CviAII CATG 1 cut(s) 172
CviQI GTAC 3 cut(s) 235, 298, 630
DdeI CTNAG 2 cut(s) 289, 458
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 69, 87
Eco57I CTGAAG 1 cut(s) 199
Eco72I CACGTG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 203
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO65I GGTNACC 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 15, 251
EcoT14I CCWWGG 2 cut(s) 69, 87
ErhI CCWWGG 2 cut(s) 69, 87
Esp3I CGTCTC 1 cut(s) 424
FaeI CATG 1 cut(s) 175
FatI CATG 1 cut(s) 171
FauNDI CATATG 1 cut(s) 411
FblI GTMKAC 1 cut(s) 475
Fnu4HI GCNGC 4 cut(s) 21, 151, 416, 539
Fsp4HI GCNGC 4 cut(s) 21, 151, 416, 539
FspBI CTAG 2 cut(s) 65, 209
GluI GCNGC 4 cut(s) 21, 151, 416, 539
GsuI CTGGAG 3 cut(s) 41, 125, 528
Hin1II CATG 1 cut(s) 175
HincII GTYRAC 1 cut(s) 476
HindII GTYRAC 1 cut(s) 476
HinfI GANTC 3 cut(s) 52, 111, 460
HphI GGTGA 3 cut(s) 113, 209, 374
Hpy166II GTNNAC 2 cut(s) 476, 630
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 2 cut(s) 58, 247
Hpy8I GTNNAC 2 cut(s) 476, 630
HpyCH4III ACNGT 4 cut(s) 101, 107, 197, 234
HpyCH4IV ACGT 2 cut(s) 180, 305
HpyCH4V TGCA 4 cut(s) 509, 557, 587, 611
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 357, 515, 554
HpyF3I CTNAG 2 cut(s) 289, 458
HpySE526I ACGT 2 cut(s) 180, 305
Hsp92II CATG 1 cut(s) 175
Kzo9I GATC 1 cut(s) 343
LmnI GCTCC 2 cut(s) 356, 499
Lsp1109I GCAGC 3 cut(s) 7, 402, 550
MabI ACCWGGT 1 cut(s) 251
MaeI CTAG 2 cut(s) 65, 209
MaeII ACGT 2 cut(s) 180, 305
MaeIII GTNAC 4 cut(s) 45, 101, 203, 380
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 3 cut(s) 70, 203, 388
MhlI GDGCHC 2 cut(s) 186, 353
MluCI AATT 2 cut(s) 8, 613
MlyI GAGTC 2 cut(s) 120, 469
MmeI TCCRAC 3 cut(s) 102, 378, 583
MnlI CCTC 2 cut(s) 296, 552
MseI TTAA 1 cut(s) 11
MspA1I CMGCKG 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 17, 253
MvaI CCWGG 2 cut(s) 17, 253
MwoI GCNNNNNNNGC 4 cut(s) 26, 357, 515, 554
NdeI CATATG 1 cut(s) 411
NdeII GATC 1 cut(s) 343
NlaIII CATG 1 cut(s) 175
NmuCI GTSAC 3 cut(s) 45, 101, 380
NspI RCATGY 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 562
PciI ACATGT 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PfeI GAWTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 4 cut(s) 22, 152, 417, 540
PleI GAGTC 2 cut(s) 119, 468
PmaCI CACGTG 1 cut(s) 306
PmlI CACGTG 1 cut(s) 306
PpsI GAGTC 2 cut(s) 119, 468
Ppu21I YACGTR 1 cut(s) 306
PscI ACATGT 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 15, 251
PspCI CACGTG 1 cut(s) 306
PspEI GGTNACC 1 cut(s) 203
PspGI CCWGG 2 cut(s) 15, 251
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 3 cut(s) 236, 299, 631
RsaNI GTAC 3 cut(s) 235, 298, 630
SalI GTCGAC 1 cut(s) 474
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 4 cut(s) 21, 151, 416, 539
Sau3AI GATC 1 cut(s) 343
SchI GAGTC 2 cut(s) 120, 469
ScrFI CCNGG 2 cut(s) 17, 253
SduI GDGCHC 2 cut(s) 186, 353
SexAI ACCWGGT 1 cut(s) 251
SfcI CTRYAG 1 cut(s) 24
Sse9I AATT 2 cut(s) 8, 613
SsiI CCGC 3 cut(s) 150, 385, 597
SspMI CTAG 2 cut(s) 65, 209
StyD4I CCNGG 2 cut(s) 15, 251
StyI CCWWGG 2 cut(s) 69, 87
TaaI ACNGT 4 cut(s) 101, 107, 197, 234
TaiI ACGT 2 cut(s) 183, 308
TaqI TCGA 4 cut(s) 186, 312, 321, 475
TasI AATT 2 cut(s) 8, 613
TatI WGTACW 2 cut(s) 234, 297
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 3 cut(s) 45, 101, 380
TseI GCWGC 3 cut(s) 20, 415, 538
Tsp45I GTSAC 3 cut(s) 45, 101, 380
TspDTI ATGAA 3 cut(s) 118, 204, 474
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 1 cut(s) 237
Van91I CCANNNNNTGG 1 cut(s) 88
XagI CCTNNNNNAGG 1 cut(s) 461
XapI RAATTY 1 cut(s) 613
XceI RCATGY 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 475
XspI CTAG 2 cut(s) 65, 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.