Prupe.1G050000_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000286
Physical Location & Seq
Forward (+)
3509559 .. 3511029
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G050000.1

Sequence Viewer

Length: 669 bp
ATGGAAGAACTACATGCAGCAGCTTATGCATACTATGACCGTGGCAATGAACATACTCGTCGTGTAGCATATGATTTCTTTCGATCCATGGATACAGACGGTGATGGCAGAATCAGCTACGCCGAGTTCAACGACTTCGTTCAACAAAGTGGATACTACTGGATCCTCCATAATGACCCCAACTTGTTCGCTAAACTTGACCGCAATCGCGACGGTGGCCTAGATTTGCAGGAAGTACTTACGTTCTACTATATCATCAAAACAAGGTACATCACATGCCAAGGCTGCCGAGCACACCTTTGTGGCTTGTATTTTACGTGCGTTGAGTGCTTTGATGGAGGAGCAGCTAATTATCATCATCATCATCAGTATCCAACGTATGACCTGTGTGCTGCTTGCTATGGTAATGCCAACTACATTAACCAGCACTCATACTTCTTGGATAACCACGTTTTGCTACGCTCCAAAAGAGGCCCTCCTCATGCTCCGCCAAATCTTGCCATGGCAGTCGCTGAACAGAATCCTCTGGTGAATATTACTAATAATTACTACGTCAATCAAGCTAGCGCTGCGCCTGAAATTCAAAGGAATAGATGGTACCAAGCATTTCAAACATTAGAGACAGCTCTTTCTATTTCAGCTTTGGCTTACAACTGCACAATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8 ZZ

Protein Analysis

223

Amino Acids

25.62

Weight (kDa)

5.96

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 597
AccB1I GGYRCC 1 cut(s) 597
AccII CGCG 1 cut(s) 210
AciI CCGC 2 cut(s) 202, 488
AclWI GGATC 3 cut(s) 78, 157, 170
AcsI RAATTY 1 cut(s) 579
AfaI GTAC 3 cut(s) 237, 269, 599
AfeI AGCGCT 1 cut(s) 568
AgsI TTSAA 4 cut(s) 130, 143, 584, 611
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 6 cut(s) 23, 117, 347, 563, 626, 641
AluI AGCT 6 cut(s) 23, 117, 347, 563, 626, 641
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 3 cut(s) 78, 157, 170
AlwNI CAGNNNCTG 1 cut(s) 512
Aor51HI AGCGCT 1 cut(s) 568
AoxI GGCC 2 cut(s) 217, 472
ApeKI GCWGC 6 cut(s) 17, 20, 285, 344, 392, 569
ApoI RAATTY 1 cut(s) 579
Asp718I GGTACC 1 cut(s) 597
AspLEI GCGC 2 cut(s) 569, 574
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 2 cut(s) 113, 541
AsuNHI GCTAGC 1 cut(s) 563
BamHI GGATCC 1 cut(s) 162
BanI GGYRCC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 6 cut(s) 29, 32, 272, 356, 379, 556
BccI CCATC 3 cut(s) 98, 329, 588
BciVI GTATCC 3 cut(s) 85, 146, 381
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 2 cut(s) 221, 564
BfoI RGCGCY 1 cut(s) 570
BfuI GTATCC 3 cut(s) 85, 146, 381
BisI GCNGC 6 cut(s) 18, 21, 286, 345, 393, 570
BlsI GCNGC 6 cut(s) 19, 22, 287, 346, 394, 571
BmcAI AGTACT 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 2 cut(s) 164, 599
BmtI GCTAGC 1 cut(s) 567
BsaAI YACGTR 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 40, 87, 280, 501
Bse1I ACTGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 52
BseDI CCNNGG 4 cut(s) 40, 87, 280, 501
BseMI GCAATG 1 cut(s) 52
BseNI ACTGG 1 cut(s) 164
BseRI GAGGAG 2 cut(s) 354, 468
BseXI GCAGC 6 cut(s) 29, 32, 272, 356, 379, 556
BsgI GTGCAG 1 cut(s) 640
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 219, 474
BshNI GGYRCC 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 295
BsmAI GTCTC 1 cut(s) 614
BsnI GGCC 2 cut(s) 219, 474
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 2 cut(s) 83, 162
Bsp19I CCATGG 2 cut(s) 87, 501
Bsp68I TCGCGA 1 cut(s) 210
BspACI CCGC 2 cut(s) 202, 488
BspANI GGCC 2 cut(s) 219, 474
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 164, 599
BspOI GCTAGC 1 cut(s) 567
BspPI GGATC 3 cut(s) 78, 157, 170
BspT107I GGYRCC 1 cut(s) 597
BsrDI GCAATG 1 cut(s) 52
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 40, 87, 280, 501
BssMI GATC 2 cut(s) 83, 162
BssT1I CCWWGG 3 cut(s) 87, 280, 501
Bst4CI ACNGT 3 cut(s) 41, 101, 215
BstAPI GCANNNNNTGC 1 cut(s) 26
BstBAI YACGTR 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 397, 565
BstDSI CCRYGG 3 cut(s) 40, 87, 501
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 570
BstHHI GCGC 2 cut(s) 569, 574
BstKTI GATC 2 cut(s) 86, 165
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 2 cut(s) 83, 162
BstMWI GCNNNNNNNGC 6 cut(s) 26, 114, 216, 285, 327, 569
BstNSI RCATGY 2 cut(s) 17, 279
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 6 cut(s) 29, 32, 272, 356, 379, 556
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BsuI GTATCC 3 cut(s) 85, 146, 381
BsuRI GGCC 2 cut(s) 219, 474
BtgI CCRYGG 3 cut(s) 40, 87, 501
BtuMI TCGCGA 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 397, 565
CaiI CAGNNNCTG 1 cut(s) 512
CfoI GCGC 2 cut(s) 569, 574
Cfr13I GGNCC 1 cut(s) 473
Csp6I GTAC 3 cut(s) 236, 268, 598
CviAII CATG 5 cut(s) 14, 88, 276, 482, 502
CviQI GTAC 3 cut(s) 236, 268, 598
DpnI GATC 2 cut(s) 85, 164
DpnII GATC 2 cut(s) 83, 162
EciI GGCGGA 1 cut(s) 477
Eco130I CCWWGG 3 cut(s) 87, 280, 501
Eco47III AGCGCT 1 cut(s) 568
EcoO109I RGGNCCY 1 cut(s) 473
EcoT14I CCWWGG 3 cut(s) 87, 280, 501
EcoT22I ATGCAT 1 cut(s) 31
ErhI CCWWGG 3 cut(s) 87, 280, 501
FaeI CATG 5 cut(s) 17, 91, 279, 485, 505
FatI CATG 5 cut(s) 13, 87, 275, 481, 501
FauNDI CATATG 1 cut(s) 70
Fnu4HI GCNGC 6 cut(s) 18, 21, 286, 345, 393, 570
Fsp4HI GCNGC 6 cut(s) 18, 21, 286, 345, 393, 570
FspBI CTAG 2 cut(s) 221, 564
GlaI GCGC 2 cut(s) 568, 573
GluI GCNGC 6 cut(s) 18, 21, 286, 345, 393, 570
HaeII RGCGCY 1 cut(s) 570
HaeIII GGCC 2 cut(s) 219, 474
HhaI GCGC 2 cut(s) 569, 574
Hin1II CATG 5 cut(s) 17, 91, 279, 485, 505
Hin6I GCGC 2 cut(s) 567, 572
HinP1I GCGC 2 cut(s) 567, 572
HinfI GANTC 2 cut(s) 111, 520
HphI GGTGA 2 cut(s) 113, 541
Hpy188III TCNNGA 1 cut(s) 209
Hpy99I CGWCG 2 cut(s) 63, 215
HpyCH4III ACNGT 3 cut(s) 41, 101, 215
HpyCH4IV ACGT 5 cut(s) 242, 317, 377, 450, 552
HpyCH4V TGCA 4 cut(s) 17, 29, 229, 657
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 114, 216, 285, 327, 569
HpySE526I ACGT 5 cut(s) 242, 317, 377, 450, 552
Hsp92II CATG 5 cut(s) 17, 91, 279, 485, 505
HspAI GCGC 2 cut(s) 567, 572
KpnI GGTACC 1 cut(s) 601
Kzo9I GATC 2 cut(s) 83, 162
LmnI GCTCC 3 cut(s) 341, 467, 490
LpnPI CCDG 6 cut(s) 145, 215, 398, 437, 512, 588
Lsp1109I GCAGC 6 cut(s) 29, 32, 272, 356, 379, 556
MaeI CTAG 2 cut(s) 221, 564
MaeII ACGT 5 cut(s) 242, 317, 377, 450, 552
MalI GATC 2 cut(s) 85, 164
MboI GATC 2 cut(s) 83, 162
MboII GAAGA 1 cut(s) 17
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 4 cut(s) 349, 544, 579, 660
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 6 cut(s) 176, 332, 464, 486, 489, 534
Mph1103I ATGCAT 1 cut(s) 31
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 2 cut(s) 300, 662
MteI GCGCNGCGC 1 cut(s) 570
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 6 cut(s) 26, 114, 216, 285, 327, 569
NcoI CCATGG 2 cut(s) 87, 501
NdeI CATATG 1 cut(s) 70
NdeII GATC 2 cut(s) 83, 162
NheI GCTAGC 1 cut(s) 563
NlaIII CATG 5 cut(s) 17, 91, 279, 485, 505
NlaIV GGNNCC 2 cut(s) 164, 599
NmeAIII GCCGAG 2 cut(s) 148, 314
NruI TCGCGA 1 cut(s) 210
NsiI ATGCAT 1 cut(s) 31
NspI RCATGY 2 cut(s) 17, 279
OliI CACNNNNGTG 1 cut(s) 300
PfeI GAWTC 2 cut(s) 111, 520
PkrI GCNGC 6 cut(s) 19, 22, 287, 346, 394, 571
Ppu21I YACGTR 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 164, 599
PspPI GGNCC 1 cut(s) 473
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 162
RruI TCGCGA 1 cut(s) 210
RsaI GTAC 3 cut(s) 237, 269, 599
RsaNI GTAC 3 cut(s) 236, 268, 598
RseI CAYNNNNRTG 2 cut(s) 300, 662
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 6 cut(s) 18, 21, 286, 345, 393, 570
Sau3AI GATC 2 cut(s) 83, 162
Sau96I GGNCC 1 cut(s) 473
ScaI AGTACT 1 cut(s) 237
SduI GDGCHC 1 cut(s) 295
SmiMI CAYNNNNRTG 2 cut(s) 300, 662
Sse9I AATT 4 cut(s) 349, 544, 579, 660
SsiI CCGC 2 cut(s) 202, 488
SspI AATATT 1 cut(s) 535
SspMI CTAG 2 cut(s) 221, 564
StyI CCWWGG 3 cut(s) 87, 280, 501
TaaI ACNGT 3 cut(s) 41, 101, 215
TaiI ACGT 5 cut(s) 245, 320, 380, 453, 555
TaqI TCGA 1 cut(s) 82
TasI AATT 4 cut(s) 349, 544, 579, 660
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 2 cut(s) 111, 520
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TseI GCWGC 6 cut(s) 17, 20, 285, 344, 392, 569
TspDTI ATGAA 1 cut(s) 63
XapI RAATTY 1 cut(s) 579
XceI RCATGY 2 cut(s) 17, 279
XspI CTAG 2 cut(s) 221, 564
ZrmI AGTACT 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.