Rw4G005080

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000286
Physical Location & Seq
Forward (+)
10279871 .. 10291232
11362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G005080.1

Sequence Viewer

Length: 849 bp
ATGGGAGACATCAGAGAAATTCGTGATGCTGCTTTAGCTAATTACCATAATTTTTCTGCAGAGGAACAGCATGAGGCGTATCTCTTTTTTCAAACAATGGATAAAGATGATGATGGAAAAATAACCATGGAGGACTTCAAGCAGAAGTTTGAGGGTTGTTTCGGCGACAATGTCAACCGTGCCTTCCAGGACCTAGACAAAAATGGAGATGGCAGTTTGGATTTTGATGAATCTATCACTCTTTACTACTGTCTTGTGGCTGAGAGGATCATTATTTGCCATTGCTGCAAAGCCTTTTTTCTAAAGGGACTGTATTTCACTTGCGTGGACTGCTTCAAGCATAACCAAACTACAACTTTTGATCTTTGCACCTCCTGCTACGACAGCCAGGACTACGCCTTGCTAAATCACGAACACTGCAACTTTTTGGACAACTACGCCTTGCTAGCTACTTATAAACTTTCTGGATTGACAAACCAGCCAGCAAGCTCAGCAAAGTCGGTTGATCCTAAGAACTATCAAGCAGACTCGGCCTTGCCCAAGGGCAGCCCTCGCAATAGCCCAGGGCTCAGGGGCAGCCCTGGCGACAGCCCAGGACCCAGGGGCAGCCCTAGCGACAGCCCTGGGCTCAAGCCAGGGCCCAAGGAAAGGACTGACTCTAGGCACCAAACAAATTTTCGCTCATGGCATCAAGAATCTCAGTACCGGCCCTCCAATGAATTCGGTTCATCTAAGACTGGAGAAGCTGTGAAAGCTGGTCTTCAATTGGGTCAAATGGCGTCAAATGCTGTGACTGTGGCCCAAGCGGTGAGCCAATTGGGTCAAGTTGCTAAATGCACCATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

282

Amino Acids

31.23

Weight (kDa)

5.48

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 29 - 82 6.2e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
AccB1I GGYRCC 1 cut(s) 663
AciI CCGC 1 cut(s) 806
AclWI GGATC 2 cut(s) 275, 500
AcsI RAATTY 3 cut(s) 18, 673, 719
AcyI GRCGYC 1 cut(s) 779
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 1 cut(s) 648
AgsI TTSAA 4 cut(s) 92, 139, 337, 764
AjnI CCWGG 8 cut(s) 186, 387, 562, 580, 592, 599, 622, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 5 cut(s) 38, 449, 489, 746, 755
AluI AGCT 5 cut(s) 38, 449, 489, 746, 755
AlwI GGATC 2 cut(s) 275, 500
AoxI GGCC 4 cut(s) 531, 638, 707, 798
ApaI GGGCCC 1 cut(s) 642
ApeKI GCWGC 5 cut(s) 29, 285, 546, 576, 606
ApoI RAATTY 3 cut(s) 18, 673, 719
AspS9I GGNCC 6 cut(s) 190, 596, 638, 639, 708, 799
AsuHPI GGTGA 1 cut(s) 820
AsuNHI GCTAGC 1 cut(s) 445
AvaII GGWCC 2 cut(s) 190, 596
BaeGI GKGCMC 1 cut(s) 642
BanI GGYRCC 1 cut(s) 663
BanII GRGCYC 3 cut(s) 570, 630, 642
BbsI GAAGAC 1 cut(s) 752
BbvI GCAGC 5 cut(s) 16, 272, 558, 588, 618
BccI CCATC 2 cut(s) 107, 203
BciT130I CCWGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
BfaI CTAG 4 cut(s) 194, 446, 612, 660
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 5 cut(s) 30, 286, 547, 577, 607
BlpI GCTNAGC 1 cut(s) 490
BlsI GCNGC 5 cut(s) 31, 287, 548, 578, 608
Bme1390I CCNGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
Bme18I GGWCC 2 cut(s) 190, 596
BmgT120I GGNCC 6 cut(s) 190, 596, 638, 639, 708, 799
BmiI GGNNCC 3 cut(s) 598, 640, 665
BmrFI CCNGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
BmsI GCATC 2 cut(s) 16, 697
BmtI GCTAGC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 752
BpmI CTGGAG 1 cut(s) 759
Bpu10I CCTNAGC 1 cut(s) 569
Bpu1102I GCTNAGC 1 cut(s) 490
BpuEI CTTGAG 1 cut(s) 614
BsaHI GRCGYC 1 cut(s) 779
BsaXI ACNNNNNCTCC 4 cut(s) 695, 725, 732, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 648
Bse118I RCCGGY 1 cut(s) 705
Bse1I ACTGG 1 cut(s) 742
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
BseLI CCNNNNNNNGG 1 cut(s) 648
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 4 cut(s) 252, 504, 583, 713
BseNI ACTGG 1 cut(s) 742
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 5 cut(s) 16, 272, 558, 588, 618
BshFI GGCC 4 cut(s) 533, 640, 709, 800
BshNI GGYRCC 1 cut(s) 663
BsiSI CCGG 1 cut(s) 706
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 648
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 4 cut(s) 533, 640, 709, 800
Bsp120I GGGCCC 1 cut(s) 638
Bsp1286I GDGCHC 3 cut(s) 570, 630, 642
Bsp143I GATC 3 cut(s) 267, 361, 505
Bsp1720I GCTNAGC 1 cut(s) 490
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 1 cut(s) 806
BspANI GGCC 4 cut(s) 533, 640, 709, 800
BspCNI CTCAG 4 cut(s) 253, 503, 582, 712
BspLI GGNNCC 3 cut(s) 598, 640, 665
BspMAI CTGCAG 1 cut(s) 61
BspOI GCTAGC 1 cut(s) 449
BspPI GGATC 2 cut(s) 275, 500
BspT107I GGYRCC 1 cut(s) 663
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 705
BsrI ACTGG 1 cut(s) 742
BssAI RCCGGY 1 cut(s) 705
BssMI GATC 3 cut(s) 267, 361, 505
BssNI GRCGYC 1 cut(s) 779
BssT1I CCWWGG 3 cut(s) 126, 540, 642
Bst2UI CCWGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
Bst4CI ACNGT 4 cut(s) 179, 251, 312, 796
BstACI GRCGYC 1 cut(s) 779
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 3 cut(s) 447, 483, 487
BstDEI CTNAG 6 cut(s) 261, 490, 510, 569, 699, 732
BstDSI CCRYGG 1 cut(s) 126
BstKTI GATC 3 cut(s) 270, 364, 508
BstMBI GATC 3 cut(s) 267, 361, 505
BstNI CCWGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
BstSCI CCNGG 8 cut(s) 186, 387, 562, 580, 592, 599, 622, 634
BstSFI CTRYAG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 642
BstV1I GCAGC 5 cut(s) 16, 272, 558, 588, 618
BstV2I GAAGAC 1 cut(s) 752
BsuRI GGCC 4 cut(s) 533, 640, 709, 800
BtgI CCRYGG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 3 cut(s) 447, 483, 487
Cfr10I RCCGGY 1 cut(s) 705
Cfr13I GGNCC 6 cut(s) 190, 596, 638, 639, 708, 799
CseI GACGC 1 cut(s) 768
Csp6I GTAC 1 cut(s) 703
CviAII CATG 3 cut(s) 71, 127, 684
CviQI GTAC 1 cut(s) 703
DdeI CTNAG 6 cut(s) 261, 490, 510, 569, 699, 732
DpnI GATC 3 cut(s) 269, 363, 507
DpnII GATC 3 cut(s) 267, 361, 505
Eco130I CCWWGG 3 cut(s) 126, 540, 642
Eco24I GRGCYC 3 cut(s) 570, 630, 642
Eco47I GGWCC 2 cut(s) 190, 596
EcoO109I RGGNCCY 3 cut(s) 190, 596, 638
EcoRI GAATTC 1 cut(s) 719
EcoRII CCWGG 8 cut(s) 186, 387, 562, 580, 592, 599, 622, 634
EcoT14I CCWWGG 3 cut(s) 126, 540, 642
EcoT38I GRGCYC 3 cut(s) 570, 630, 642
ErhI CCWWGG 3 cut(s) 126, 540, 642
FaeI CATG 3 cut(s) 74, 130, 687
FaiI YATR 7 cut(s) 48, 72, 128, 342, 456, 685, 845
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 1 cut(s) 321
FatI CATG 3 cut(s) 70, 126, 683
Fnu4HI GCNGC 5 cut(s) 30, 286, 547, 577, 607
FriOI GRGCYC 3 cut(s) 570, 630, 642
Fsp4HI GCNGC 5 cut(s) 30, 286, 547, 577, 607
FspBI CTAG 4 cut(s) 194, 446, 612, 660
GluI GCNGC 5 cut(s) 30, 286, 547, 577, 607
GsuI CTGGAG 1 cut(s) 759
HaeIII GGCC 4 cut(s) 533, 640, 709, 800
HapII CCGG 1 cut(s) 706
HgaI GACGC 1 cut(s) 768
Hin1I GRCGYC 1 cut(s) 779
Hin1II CATG 3 cut(s) 74, 130, 687
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 4 cut(s) 230, 527, 656, 695
HpaII CCGG 1 cut(s) 706
HphI GGTGA 1 cut(s) 820
Hpy166II GTNNAC 2 cut(s) 175, 328
Hpy188I TCNGA 1 cut(s) 14
Hpy188III TCNNGA 4 cut(s) 23, 410, 465, 692
Hpy8I GTNNAC 2 cut(s) 175, 328
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 4 cut(s) 179, 251, 312, 796
HpyCH4V TGCA 5 cut(s) 59, 288, 369, 420, 837
HpyF3I CTNAG 6 cut(s) 261, 490, 510, 569, 699, 732
Hsp92I GRCGYC 1 cut(s) 779
Hsp92II CATG 3 cut(s) 74, 130, 687
Kzo9I GATC 3 cut(s) 267, 361, 505
Lsp1109I GCAGC 5 cut(s) 16, 272, 558, 588, 618
LweI GCATC 2 cut(s) 16, 697
MaeI CTAG 4 cut(s) 194, 446, 612, 660
MaeIII GTNAC 1 cut(s) 790
MalI GATC 3 cut(s) 269, 363, 507
MboI GATC 3 cut(s) 267, 361, 505
MboII GAAGA 1 cut(s) 752
MfeI CAATTG 2 cut(s) 764, 815
MhlI GDGCHC 3 cut(s) 570, 630, 642
MluCI AATT 7 cut(s) 18, 40, 49, 673, 719, 764, 815
MlyI GAGTC 2 cut(s) 521, 650
MnlI CCTC 8 cut(s) 55, 67, 124, 145, 258, 382, 561, 721
MslI CAYNNNNRTG 2 cut(s) 323, 842
MspI CCGG 1 cut(s) 706
MspR9I CCNGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
MunI CAATTG 2 cut(s) 764, 815
MvaI CCWGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
NcoI CCATGG 1 cut(s) 126
NdeII GATC 3 cut(s) 267, 361, 505
NheI GCTAGC 1 cut(s) 445
NlaIII CATG 3 cut(s) 74, 130, 687
NlaIV GGNNCC 3 cut(s) 598, 640, 665
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 1 cut(s) 790
OliI CACNNNNGTG 1 cut(s) 323
PasI CCCWGGG 3 cut(s) 563, 600, 623
PfeI GAWTC 2 cut(s) 230, 695
PflFI GACNNNGTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 5 cut(s) 31, 287, 548, 578, 608
PleI GAGTC 2 cut(s) 521, 650
PpsI GAGTC 2 cut(s) 521, 650
PpuMI RGGWCCY 2 cut(s) 190, 596
PsiI TTATAA 1 cut(s) 456
Psp5II RGGWCCY 2 cut(s) 190, 596
Psp6I CCWGG 8 cut(s) 186, 387, 562, 580, 592, 599, 622, 634
PspGI CCWGG 8 cut(s) 186, 387, 562, 580, 592, 599, 622, 634
PspN4I GGNNCC 3 cut(s) 598, 640, 665
PspOMI GGGCCC 1 cut(s) 638
PspPI GGNCC 6 cut(s) 190, 596, 638, 639, 708, 799
PspPPI RGGWCCY 2 cut(s) 190, 596
PstI CTGCAG 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
RseI CAYNNNNRTG 2 cut(s) 323, 842
SatI GCNGC 5 cut(s) 30, 286, 547, 577, 607
Sau3AI GATC 3 cut(s) 267, 361, 505
Sau96I GGNCC 6 cut(s) 190, 596, 638, 639, 708, 799
SchI GAGTC 2 cut(s) 521, 650
ScrFI CCNGG 8 cut(s) 188, 389, 564, 582, 594, 601, 624, 636
SduI GDGCHC 3 cut(s) 570, 630, 642
SetI ASST 7 cut(s) 40, 195, 374, 451, 491, 748, 757
SfaNI GCATC 2 cut(s) 16, 697
SfcI CTRYAG 1 cut(s) 57
SinI GGWCC 2 cut(s) 190, 596
SmiMI CAYNNNNRTG 2 cut(s) 323, 842
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
Sse9I AATT 7 cut(s) 18, 40, 49, 673, 719, 764, 815
SsiI CCGC 1 cut(s) 806
SspMI CTAG 4 cut(s) 194, 446, 612, 660
StyD4I CCNGG 8 cut(s) 186, 387, 562, 580, 592, 599, 622, 634
StyI CCWWGG 3 cut(s) 126, 540, 642
TaaI ACNGT 4 cut(s) 179, 251, 312, 796
TasI AATT 7 cut(s) 18, 40, 49, 673, 719, 764, 815
TfiI GAWTC 2 cut(s) 230, 695
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 1 cut(s) 790
TseI GCWGC 5 cut(s) 29, 285, 546, 576, 606
Tsp45I GTSAC 1 cut(s) 790
TspDTI ATGAA 3 cut(s) 243, 717, 732
TspRI CASTG 1 cut(s) 422
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 190, 596
XapI RAATTY 3 cut(s) 18, 673, 719
XspI CTAG 4 cut(s) 194, 446, 612, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.