FvH4_4g04850

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000286
Physical Location & Seq
Forward (+)
4220176 .. 4222196
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04850.t1

Sequence Viewer

Length: 675 bp
ATGGAAGAAATACGTGAGGCAGCTTTAGCTTACTACCATAACTTGCCAGAGGATCTACAGAAGCTGGCATCTGCAAAATTCAAAGAAATTGATTCAAATGGTAATGGAACAATAAGCTTGAGGGAATTCAAGAACAGTATGCGAAGCTCTTTCGACAACGACTCCGTGATCATGAAGAGAAGCTTCAAGGAGCTGGACAAGAACGGAGACGGCAAGCTGGATTTCAACGAATACATCACTCTTTACTATCTTTTGGAGAGCGGTAGGCTGCTGATTTATTGTGCGGGAGATGGATGTGAGGACGACGCACCTTTTCTCAAGGGACACTATTTCACCTGCGTCGACTGCTTCCACCATAACGAAAATGAAACTTTCGATCTCTGCACCTCCTGCTACCGCAATGGAAAATTTGCTCATGAACACACCAACTTTTTGGATAATCATGTCTTACTTCGTTCCAAGGCTGCGTGTTGTGATGAAGCCATATCTGAGCCAAGCTCTTCAGGGTCTGATTCTGAGTCCAATCAAGTTAGTCGATCTGGTTCACAGAGAGAGCAAGTTAATCGATCTGGATCAAAGAGGAGGGCAGTATTTGGAGCAGTAAAATCTGGATTGAGCGTTGCGCAAGCAGTAGGAAGTGTTGCTTCTGATGTTGCTTCGTGCAGCATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

225

Amino Acids

24.84

Weight (kDa)

5.39

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 21 - 83 2.2e-11 EF-hand domain pair
EF-hand_1 PF00036 58 - 81 6e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 344
Acc16I TGCGCA 1 cut(s) 624
Acc36I ACCTGC 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 342
AciI CCGC 3 cut(s) 261, 284, 397
AclWI GGATC 2 cut(s) 60, 580
AcsI RAATTY 3 cut(s) 77, 125, 407
AcuI CTGAAG 1 cut(s) 486
AgsI TTSAA 5 cut(s) 82, 96, 130, 187, 226
AluBI AGCT 9 cut(s) 23, 29, 64, 117, 147, 183, 193, 217, 498
AluI AGCT 9 cut(s) 23, 29, 64, 117, 147, 183, 193, 217, 498
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 2 cut(s) 60, 580
AlwNI CAGNNNCTG 2 cut(s) 64, 509
ApeKI GCWGC 4 cut(s) 20, 268, 464, 663
ApoI RAATTY 3 cut(s) 77, 125, 407
AspLEI GCGC 1 cut(s) 625
AsuHPI GGTGA 1 cut(s) 325
BbvI GCAGC 3 cut(s) 32, 255, 451
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 226
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 4 cut(s) 21, 269, 465, 664
BlsI GCNGC 4 cut(s) 22, 270, 466, 665
BmsI GCATC 1 cut(s) 77
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpuEI CTTGAG 2 cut(s) 139, 302
Bsa29I ATCGAT 1 cut(s) 565
BsaAI YACGTR 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 571
BsaJI CCNNGG 1 cut(s) 459
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse3DI GCAATG 1 cut(s) 406
Bse8I GATNNNNATC 1 cut(s) 571
BseCI ATCGAT 1 cut(s) 565
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 299
BseJI GATNNNNATC 1 cut(s) 571
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 480, 507
BseRI GAGGAG 1 cut(s) 595
BseXI GCAGC 3 cut(s) 32, 255, 451
BsgI GTGCAG 1 cut(s) 367
BshVI ATCGAT 1 cut(s) 565
BslFI GGGAC 1 cut(s) 336
BsmAI GTCTC 1 cut(s) 201
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 336
Bsp143I GATC 6 cut(s) 52, 168, 376, 536, 566, 572
BspACI CCGC 3 cut(s) 261, 284, 397
BspCNI CTCAG 2 cut(s) 481, 508
BspDI ATCGAT 1 cut(s) 565
BspHI TCATGA 2 cut(s) 171, 415
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 2 cut(s) 60, 580
BspQI GCTCTTC 1 cut(s) 505
BsrBI CCGCTC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 6 cut(s) 52, 168, 376, 536, 566, 572
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 137
Bst6I CTCTTC 2 cut(s) 170, 505
BstAPI GCANNNNNTGC 1 cut(s) 390
BstBAI YACGTR 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 66, 215, 627
BstDEI CTNAG 2 cut(s) 489, 516
BstF5I GGATG 1 cut(s) 299
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 6 cut(s) 55, 171, 379, 539, 569, 575
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 6 cut(s) 52, 168, 376, 536, 566, 572
BstMWI GCNNNNNNNGC 3 cut(s) 26, 345, 390
BstSFI CTRYAG 1 cut(s) 56
BstV1I GCAGC 3 cut(s) 32, 255, 451
BstX2I RGATCY 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 565
BsuTUI ATCGAT 1 cut(s) 565
BtsCI GGATG 1 cut(s) 299
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 3 cut(s) 66, 215, 627
CaiI CAGNNNCTG 2 cut(s) 64, 509
CciI TCATGA 2 cut(s) 171, 415
CfoI GCGC 1 cut(s) 625
ClaI ATCGAT 1 cut(s) 565
CseI GACGC 2 cut(s) 314, 328
CviAII CATG 4 cut(s) 172, 416, 443, 670
DdeI CTNAG 2 cut(s) 489, 516
DpnI GATC 6 cut(s) 54, 170, 378, 538, 568, 574
DpnII GATC 6 cut(s) 52, 168, 376, 536, 566, 572
Eam1104I CTCTTC 2 cut(s) 170, 505
EarI CTCTTC 2 cut(s) 170, 505
Eco130I CCWWGG 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 486
EcoRI GAATTC 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 201
FaeI CATG 4 cut(s) 175, 419, 446, 673
FaiI YATR 8 cut(s) 39, 140, 173, 357, 417, 444, 485, 671
FalI AAGNNNNNCTT 4 cut(s) 167, 199, 628, 660
FaqI GGGAC 1 cut(s) 336
FatI CATG 4 cut(s) 171, 415, 442, 669
FauI CCCGC 1 cut(s) 277
FbaI TGATCA 1 cut(s) 168
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 21, 269, 465, 664
FokI GGATG 1 cut(s) 306
Fsp4HI GCNGC 4 cut(s) 21, 269, 465, 664
FspI TGCGCA 1 cut(s) 624
GlaI GCGC 1 cut(s) 624
GluI GCNGC 4 cut(s) 21, 269, 465, 664
HgaI GACGC 2 cut(s) 314, 328
HhaI GCGC 1 cut(s) 625
Hin1II CATG 4 cut(s) 175, 419, 446, 673
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 2 cut(s) 115, 181
HinfI GANTC 4 cut(s) 92, 161, 512, 518
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 2 cut(s) 343, 545
Hpy188I TCNGA 4 cut(s) 490, 511, 517, 649
Hpy188III TCNNGA 5 cut(s) 130, 172, 416, 570, 609
Hpy8I GTNNAC 2 cut(s) 343, 545
Hpy99I CGWCG 2 cut(s) 308, 344
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 3 cut(s) 74, 384, 663
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 345, 390
HpyF3I CTNAG 2 cut(s) 489, 516
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 4 cut(s) 175, 419, 446, 673
HspAI GCGC 1 cut(s) 623
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 6 cut(s) 52, 168, 376, 536, 566, 572
LguI GCTCTTC 1 cut(s) 505
LmnI GCTCC 2 cut(s) 190, 596
Lsp1109I GCAGC 3 cut(s) 32, 255, 451
LweI GCATC 1 cut(s) 77
MaeII ACGT 1 cut(s) 13
MalI GATC 6 cut(s) 54, 170, 378, 538, 568, 574
MbiI CCGCTC 1 cut(s) 261
MboI GATC 6 cut(s) 52, 168, 376, 536, 566, 572
MboII GAAGA 3 cut(s) 17, 187, 492
MflI RGATCY 1 cut(s) 52
MluCI AATT 4 cut(s) 77, 87, 125, 407
MlyI GAGTC 2 cut(s) 155, 527
MnlI CCTC 7 cut(s) 10, 43, 114, 292, 397, 573, 576
MseI TTAA 1 cut(s) 561
MwoI GCNNNNNNNGC 3 cut(s) 26, 345, 390
NdeII GATC 6 cut(s) 52, 168, 376, 536, 566, 572
NlaIII CATG 4 cut(s) 175, 419, 446, 673
NsbI TGCGCA 1 cut(s) 624
PagI TCATGA 2 cut(s) 171, 415
PaqCI CACCTGC 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 505
PfeI GAWTC 2 cut(s) 92, 512
PkrI GCNGC 4 cut(s) 22, 270, 466, 665
PleI GAGTC 2 cut(s) 155, 526
PpsI GAGTC 2 cut(s) 155, 526
Ppu21I YACGTR 1 cut(s) 14
PstNI CAGNNNCTG 2 cut(s) 64, 509
PsuI RGATCY 1 cut(s) 52
SalI GTCGAC 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 505
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 4 cut(s) 21, 269, 465, 664
Sau3AI GATC 6 cut(s) 52, 168, 376, 536, 566, 572
SchI GAGTC 2 cut(s) 155, 527
SfaNI GCATC 1 cut(s) 77
SfcI CTRYAG 1 cut(s) 56
SmlI CTYRAG 2 cut(s) 118, 317
SmoI CTYRAG 2 cut(s) 118, 317
Sse9I AATT 4 cut(s) 77, 87, 125, 407
SsiI CCGC 3 cut(s) 261, 284, 397
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 137
TaiI ACGT 1 cut(s) 16
TaqI TCGA 5 cut(s) 153, 342, 375, 535, 565
TasI AATT 4 cut(s) 77, 87, 125, 407
TfiI GAWTC 2 cut(s) 92, 512
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TseI GCWGC 4 cut(s) 20, 268, 464, 663
TspDTI ATGAA 4 cut(s) 188, 381, 432, 492
TspGWI ACGGA 2 cut(s) 154, 219
XapI RAATTY 3 cut(s) 77, 125, 407
XmiI GTMKAC 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.