Rw5G015940

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000286
Physical Location & Seq
Reverse (-)
19845927 .. 19847012
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015940.1

Sequence Viewer

Length: 564 bp
ATGGACGAGTTGCATAAGGCAGCTTCAGCATATTACAACAACGGGTCGCCTGAGATTCAGCAACTAGCATGGAGCTTCTTCCAATCCATGGACACAAACTGCGACGGCCAAGTTGACTGGAACGAGTTCTCCGCATTTCTCTACCAAAGCGGCAACCAATGGATCAGCCCCAGCTTCTTCAACGATCTGGACCGGAACCACGACGGTGCTCTCGATTTCATGGAAACTAGGAGCTTCTGGTGCAGCGTCTGCGGAATTTGTCTCACGGGGCTCTACTTCACTTGTGTTGAGTGCTTTGACAACGTGAACTCAAACACTTACGATCTTTGTCCCAGATGCTATGAGTCGAAGCCGGAAAGGCAAAGGTATAGCCAGCAGAGTGATCACCATCATGACTACTTTATGGATAACCATATGTTGCTCAGATCAAAGAGAGGAGGATATGGTTCTGCAAACCTGGCCATAGATTCACCTGGAGGTGGACCAGGACGGACCAGAATGCGCCAAACGATGAAAGCATTGGATACGGCAGTGAATGTGGCAACTTTGTGTAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_8 ZZ

Protein Analysis

187

Amino Acids

21.34

Weight (kDa)

5.07

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 3 cut(s) 132, 150, 252
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 2 cut(s) 106, 459
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 9
AfiI CCNNNNNNNGG 2 cut(s) 88, 479
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 3 cut(s) 456, 472, 484
AleI CACNNNNGTG 1 cut(s) 204
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 4 cut(s) 23, 75, 174, 234
AluI AGCT 4 cut(s) 23, 75, 174, 234
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 249
AoxI GGCC 2 cut(s) 106, 459
ApeKI GCWGC 2 cut(s) 20, 243
ApoI RAATTY 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 504
AspS9I GGNCC 3 cut(s) 190, 482, 492
AsuHPI GGTGA 2 cut(s) 377, 462
AvaII GGWCC 3 cut(s) 190, 482, 492
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BalI TGGCCA 1 cut(s) 461
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 32, 255
BccI CCATC 1 cut(s) 396
BceAI ACGGC 2 cut(s) 121, 543
BciT130I CCWGG 3 cut(s) 458, 474, 486
BciVI GTATCC 1 cut(s) 517
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 2 cut(s) 65, 228
BfuI GTATCC 1 cut(s) 517
BglI GCCNNNNNGGC 1 cut(s) 358
BisI GCNGC 3 cut(s) 21, 151, 244
BlsI GCNGC 3 cut(s) 22, 152, 245
Bme1390I CCNGG 3 cut(s) 458, 474, 486
Bme18I GGWCC 3 cut(s) 190, 482, 492
BmgT120I GGNCC 3 cut(s) 190, 482, 492
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 3 cut(s) 458, 474, 486
BmsI GCATC 1 cut(s) 326
BpmI CTGGAG 1 cut(s) 495
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 479
Bse1I ACTGG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 3 cut(s) 458, 474, 486
BseDI CCNNGG 1 cut(s) 87
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 88, 479
BseMII CTCAG 2 cut(s) 42, 436
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 450
BseXI GCAGC 2 cut(s) 32, 255
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 262
BshFI GGCC 2 cut(s) 108, 461
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 2 cut(s) 193, 353
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 2 cut(s) 88, 479
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 504
BsnI GGCC 2 cut(s) 108, 461
Bsp1286I GDGCHC 2 cut(s) 211, 273
Bsp143I GATC 5 cut(s) 162, 184, 322, 382, 425
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 3 cut(s) 132, 150, 252
BspANI GGCC 2 cut(s) 108, 461
BspCNI CTCAG 2 cut(s) 43, 435
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 5 cut(s) 162, 184, 322, 382, 425
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 458, 474, 486
Bst4CI ACNGT 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 374
BstDEI CTNAG 2 cut(s) 51, 422
BstDSI CCRYGG 1 cut(s) 87
BstHHI GCGC 1 cut(s) 504
BstKTI GATC 5 cut(s) 165, 187, 325, 385, 428
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 5 cut(s) 162, 184, 322, 382, 425
BstMWI GCNNNNNNNGC 5 cut(s) 26, 240, 249, 358, 458
BstNI CCWGG 3 cut(s) 458, 474, 486
BstSCI CCNGG 3 cut(s) 456, 472, 484
BstV1I GCAGC 2 cut(s) 32, 255
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 2 cut(s) 108, 461
BtgI CCRYGG 1 cut(s) 87
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 1 cut(s) 537
Cac8I GCNNGC 1 cut(s) 374
CaiI CAGNNNCTG 1 cut(s) 249
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 504
Cfr13I GGNCC 3 cut(s) 190, 482, 492
CseI GACGC 1 cut(s) 235
CviAII CATG 4 cut(s) 69, 88, 220, 392
DdeI CTNAG 2 cut(s) 51, 422
DpnI GATC 5 cut(s) 164, 186, 324, 384, 427
DpnII GATC 5 cut(s) 162, 184, 322, 382, 425
EaeI YGGCCR 2 cut(s) 106, 459
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 3 cut(s) 190, 482, 492
Eco57I CTGAAG 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 456, 472, 484
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 4 cut(s) 72, 91, 223, 395
FaqI GGGAC 1 cut(s) 315
FatI CATG 4 cut(s) 68, 87, 219, 391
FauNDI CATATG 1 cut(s) 414
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 3 cut(s) 21, 151, 244
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 3 cut(s) 21, 151, 244
FspBI CTAG 2 cut(s) 65, 228
GlaI GCGC 1 cut(s) 503
GluI GCNGC 3 cut(s) 21, 151, 244
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 2 cut(s) 108, 461
HapII CCGG 2 cut(s) 193, 353
HgaI GACGC 1 cut(s) 235
HhaI GCGC 1 cut(s) 504
Hin1II CATG 4 cut(s) 72, 91, 223, 395
Hin6I GCGC 1 cut(s) 502
HinP1I GCGC 1 cut(s) 502
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 3 cut(s) 55, 344, 467
HpaII CCGG 2 cut(s) 193, 353
HphI GGTGA 2 cut(s) 377, 462
Hpy166II GTNNAC 3 cut(s) 115, 307, 482
Hpy188I TCNGA 1 cut(s) 425
Hpy188III TCNNGA 3 cut(s) 188, 212, 392
Hpy8I GTNNAC 3 cut(s) 115, 307, 482
Hpy99I CGWCG 2 cut(s) 107, 206
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 3 cut(s) 13, 243, 452
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 240, 249, 358, 458
HpyF3I CTNAG 2 cut(s) 51, 422
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 4 cut(s) 72, 91, 223, 395
HspAI GCGC 1 cut(s) 502
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 162, 184, 322, 382, 425
LmnI GCTCC 2 cut(s) 72, 231
Lsp1109I GCAGC 2 cut(s) 32, 255
LweI GCATC 1 cut(s) 326
MaeI CTAG 2 cut(s) 65, 228
MaeII ACGT 1 cut(s) 303
MalI GATC 5 cut(s) 164, 186, 324, 384, 427
MboI GATC 5 cut(s) 162, 184, 322, 382, 425
MboII GAAGA 2 cut(s) 70, 169
MhlI GDGCHC 2 cut(s) 211, 273
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 1 cut(s) 353
MnlI CCTC 3 cut(s) 428, 431, 470
Mox20I TGGCCA 1 cut(s) 461
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 461
MslI CAYNNNNRTG 2 cut(s) 204, 390
Msp20I TGGCCA 1 cut(s) 461
MspI CCGG 2 cut(s) 193, 353
MspR9I CCNGG 3 cut(s) 458, 474, 486
Mva1269I GAATGC 1 cut(s) 504
MvaI CCWGG 3 cut(s) 458, 474, 486
MwoI GCNNNNNNNGC 5 cut(s) 26, 240, 249, 358, 458
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 1 cut(s) 414
NdeII GATC 5 cut(s) 162, 184, 322, 382, 425
NlaIII CATG 4 cut(s) 72, 91, 223, 395
NlaIV GGNNCC 1 cut(s) 197
OliI CACNNNNGTG 1 cut(s) 204
PagI TCATGA 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 504
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 2 cut(s) 55, 467
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 3 cut(s) 22, 152, 245
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 456, 472, 484
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 3 cut(s) 456, 472, 484
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 3 cut(s) 190, 482, 492
PstNI CAGNNNCTG 1 cut(s) 249
RseI CAYNNNNRTG 2 cut(s) 204, 390
SatI GCNGC 3 cut(s) 21, 151, 244
Sau3AI GATC 5 cut(s) 162, 184, 322, 382, 425
Sau96I GGNCC 3 cut(s) 190, 482, 492
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 3 cut(s) 458, 474, 486
SduI GDGCHC 2 cut(s) 211, 273
SetI ASST 9 cut(s) 25, 77, 176, 236, 306, 368, 459, 475, 481
SfaNI GCATC 1 cut(s) 326
SinI GGWCC 3 cut(s) 190, 482, 492
SmiMI CAYNNNNRTG 2 cut(s) 204, 390
Sse9I AATT 1 cut(s) 255
SsiI CCGC 3 cut(s) 132, 150, 252
SspMI CTAG 2 cut(s) 65, 228
StyD4I CCNGG 3 cut(s) 456, 472, 484
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 1 cut(s) 306
TaqI TCGA 2 cut(s) 213, 347
TasI AATT 1 cut(s) 255
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 55, 467
TscAI CASTG 1 cut(s) 537
TseI GCWGC 2 cut(s) 20, 243
TspDTI ATGAA 2 cut(s) 208, 527
TspGWI ACGGA 1 cut(s) 505
TspRI CASTG 1 cut(s) 537
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 3 cut(s) 190, 482, 492
XapI RAATTY 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 2 cut(s) 65, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.