RLG00000009594

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000286
Physical Location & Seq
Reverse (-)
55075907 .. 55077265
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009594

Sequence Viewer

Length: 654 bp
ATGGAAGAACTACATGCAGCTGCTTTAGCATACTACGAGAATGGCACTCAAAGGACTTGGGATCTGGCATGGTCTTTCTTCCAATCTATGGACACCAACCGTGATGGTCGAATCAGCCGCTTGGAGTTCGATGACTTCCTCCAGCAAAGTGGCTACAACTGGATTATTAACGACCCAAACCTCTTCGAAAGACTCGACCTCAATAGGGATGGTGGATTGGATTTTGGGGAAGTTCTCACTTTCTACTACATCATTAAAACGAGAATCCTCCGATGCCAAGGGTGTCAGTGCGGCGCACAGTACCTCTTCGGTCTGTATTTCACTTGCGTTGCTTGCTTTGACGTAGCTCATGGGCATACCTTTGATCTCTGTCCTGCCTGCTATCGCAATCGGAGTTACCAGCACCACCAGCACCACACATACTTCTTGGATAATCATGTATTGCTGCGCTCCAAACGAGGCCTCTCTCCTCAACCAAATCTCAACATGGCAATCACCACTCGACCCCCAGCCCCGCCTCCACCAGTTAATACTGTCATTAATAATTACTACATTGCTGCTGAACCCCAAAGGCCTTATTGGTTCCAATTTTTTAGTGCGTTTGAGGCGGTTCTTACTGCTGCAAGTGTGGTTTCTAATTGTACAATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

218

Amino Acids

25.15

Weight (kDa)

6.03

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 25 - 83 8.7e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 88
AciI CCGC 4 cut(s) 118, 291, 515, 608
AclWI GGATC 1 cut(s) 69
AfaI GTAC 2 cut(s) 302, 643
AfiI CCNNNNNNNGG 2 cut(s) 88, 205
AluBI AGCT 2 cut(s) 20, 347
AluI AGCT 2 cut(s) 20, 347
AlwI GGATC 1 cut(s) 69
AoxI GGCC 2 cut(s) 460, 572
ApeKI GCWGC 5 cut(s) 17, 20, 445, 557, 620
AseI ATTAAT 1 cut(s) 540
AspLEI GCGC 2 cut(s) 296, 450
AsuHPI GGTGA 1 cut(s) 487
AsuII TTCGAA 1 cut(s) 186
BbvI GCAGC 5 cut(s) 7, 29, 432, 544, 607
BccI CCATC 2 cut(s) 98, 203
BisI GCNGC 7 cut(s) 18, 21, 118, 292, 446, 558, 621
BlsI GCNGC 7 cut(s) 19, 22, 119, 293, 447, 559, 622
BmiI GGNNCC 1 cut(s) 584
BmsI GCATC 1 cut(s) 263
BpmI CTGGAG 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 277
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 205
Bse1I ACTGG 2 cut(s) 164, 524
Bse3DI GCAATG 1 cut(s) 552
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 88, 205
BseMI GCAATG 1 cut(s) 552
BseNI ACTGG 2 cut(s) 164, 524
BseRI GAGGAG 1 cut(s) 459
BseXI GCAGC 5 cut(s) 7, 29, 432, 544, 607
BseYI CCCAGC 1 cut(s) 508
BshFI GGCC 2 cut(s) 462, 574
BslI CCNNNNNNNGG 2 cut(s) 88, 205
BsnI GGCC 2 cut(s) 462, 574
Bsp119I TTCGAA 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 641
Bsp143I GATC 2 cut(s) 61, 364
BspACI CCGC 4 cut(s) 118, 291, 515, 608
BspANI GGCC 2 cut(s) 462, 574
BspLI GGNNCC 1 cut(s) 584
BspPI GGATC 1 cut(s) 69
BspT104I TTCGAA 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 641
BsrI ACTGG 2 cut(s) 164, 524
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 2 cut(s) 61, 364
BssT1I CCWWGG 1 cut(s) 277
Bst4CI ACNGT 3 cut(s) 101, 300, 535
Bst6I CTCTTC 2 cut(s) 188, 311
BstAUI TGTACA 1 cut(s) 641
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 334, 379
BstF5I GGATG 1 cut(s) 214
BstHHI GCGC 2 cut(s) 296, 450
BstKTI GATC 2 cut(s) 64, 367
BstMBI GATC 2 cut(s) 61, 364
BstMWI GCNNNNNNNGC 4 cut(s) 26, 333, 409, 605
BstNSI RCATGY 1 cut(s) 17
BstV1I GCAGC 5 cut(s) 7, 29, 432, 544, 607
BstX2I RGATCY 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 149
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 2 cut(s) 462, 574
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 334, 379
CfoI GCGC 2 cut(s) 296, 450
Csp6I GTAC 2 cut(s) 301, 642
CviAII CATG 6 cut(s) 14, 69, 350, 437, 487, 649
CviJI RGCY 7 cut(s) 20, 117, 153, 347, 462, 512, 574
CviKI_1 RGCY 7 cut(s) 20, 117, 153, 347, 462, 512, 574
CviQI GTAC 2 cut(s) 301, 642
DpnI GATC 2 cut(s) 63, 366
DpnII GATC 2 cut(s) 61, 364
Eam1104I CTCTTC 2 cut(s) 188, 311
EarI CTCTTC 2 cut(s) 188, 311
Eco130I CCWWGG 1 cut(s) 277
Eco147I AGGCCT 2 cut(s) 462, 574
EcoT14I CCWWGG 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 6 cut(s) 17, 72, 353, 440, 490, 652
FatI CATG 6 cut(s) 13, 68, 349, 436, 486, 648
FauI CCCGC 1 cut(s) 522
Fnu4HI GCNGC 7 cut(s) 18, 21, 118, 292, 446, 558, 621
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 7 cut(s) 18, 21, 118, 292, 446, 558, 621
GlaI GCGC 2 cut(s) 295, 449
GluI GCNGC 7 cut(s) 18, 21, 118, 292, 446, 558, 621
GsaI CCCAGC 1 cut(s) 512
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 2 cut(s) 462, 574
HhaI GCGC 2 cut(s) 296, 450
Hin1II CATG 6 cut(s) 17, 72, 353, 440, 490, 652
Hin6I GCGC 2 cut(s) 294, 448
HinP1I GCGC 2 cut(s) 294, 448
HinfI GANTC 3 cut(s) 111, 192, 264
HphI GGTGA 1 cut(s) 487
Hpy188I TCNGA 2 cut(s) 272, 393
HpyCH4III ACNGT 3 cut(s) 101, 300, 535
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 2 cut(s) 17, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 333, 409, 605
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 6 cut(s) 17, 72, 353, 440, 490, 652
HspAI GCGC 2 cut(s) 294, 448
Kzo9I GATC 2 cut(s) 61, 364
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 9 cut(s) 50, 145, 155, 387, 391, 413, 422, 522, 537
Lsp1109I GCAGC 5 cut(s) 7, 29, 432, 544, 607
LweI GCATC 1 cut(s) 263
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 1 cut(s) 395
MalI GATC 2 cut(s) 63, 366
MboI GATC 2 cut(s) 61, 364
MboII GAAGA 4 cut(s) 17, 70, 175, 298
MflI RGATCY 1 cut(s) 61
MluCI AATT 3 cut(s) 544, 587, 637
MlyI GAGTC 1 cut(s) 186
MseI TTAA 4 cut(s) 168, 255, 528, 540
MspA1I CMGCKG 1 cut(s) 20
MwoI GCNNNNNNNGC 4 cut(s) 26, 333, 409, 605
NdeII GATC 2 cut(s) 61, 364
NlaIII CATG 6 cut(s) 17, 72, 353, 440, 490, 652
NlaIV GGNNCC 1 cut(s) 584
NspI RCATGY 1 cut(s) 17
NspV TTCGAA 1 cut(s) 186
PceI AGGCCT 2 cut(s) 462, 574
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PfeI GAWTC 2 cut(s) 111, 264
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 7 cut(s) 19, 22, 119, 293, 447, 559, 622
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
PshBI ATTAAT 1 cut(s) 540
PspFI CCCAGC 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 584
PsuI RGATCY 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 302, 643
RsaNI GTAC 2 cut(s) 301, 642
SaqAI TTAA 4 cut(s) 168, 255, 528, 540
SatI GCNGC 7 cut(s) 18, 21, 118, 292, 446, 558, 621
Sau3AI GATC 2 cut(s) 61, 364
SchI GAGTC 1 cut(s) 186
SetI ASST 7 cut(s) 22, 183, 201, 306, 345, 349, 362
SfaNI GCATC 1 cut(s) 263
SfuI TTCGAA 1 cut(s) 186
Sse9I AATT 3 cut(s) 544, 587, 637
SseBI AGGCCT 2 cut(s) 462, 574
SsiI CCGC 4 cut(s) 118, 291, 515, 608
StuI AGGCCT 2 cut(s) 462, 574
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 3 cut(s) 101, 300, 535
TaiI ACGT 1 cut(s) 345
TaqI TCGA 5 cut(s) 109, 129, 186, 195, 502
TaqII GACCGA 1 cut(s) 299
TasI AATT 3 cut(s) 544, 587, 637
TatI WGTACW 1 cut(s) 641
TauI GCSGC 2 cut(s) 120, 294
TfiI GAWTC 2 cut(s) 111, 264
Tru1I TTAA 4 cut(s) 168, 255, 528, 540
Tru9I TTAA 4 cut(s) 168, 255, 528, 540
TscAI CASTG 1 cut(s) 293
TseI GCWGC 5 cut(s) 17, 20, 445, 557, 620
TspRI CASTG 1 cut(s) 293
Van91I CCANNNNNTGG 1 cut(s) 88
VspI ATTAAT 1 cut(s) 540
XceI RCATGY 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.