RchiOBHm_Chr4g0395781

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000286
Physical Location & Seq
Forward (+)
11729372 .. 11729686
315 bp
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UTR
Exon/CDS
Intron
PRQ36820

Sequence Viewer

Length: 315 bp
ATGGTCAAATCGATCAGCTGCTTGGAATTCAATGACTTCCTCCAGCAAAGCGGCTACAACTGGATTATTAACGACCCAAACTTCTTCAAAAGGCTCGACCGTAATGGCGATAATGGATTGGATTTTGGGGAAGCTCTCATATTCTACTACATCATTAAAACGAGGTACATCCGATGCCAAGGGTACCAGTGCAGCGTACACCTCTGCGGTTTGTATTTCACTTGCGTTGGTTGCTTTGATGAGGCTCATAAACATCGCTCCACCTTTGATCTATGTCCTGCCTGCTATCGCAATTGGAATTACTATCATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

104

Amino Acids

12.43

Weight (kDa)

6.92

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 183
AciI CCGC 2 cut(s) 51, 207
AcsI RAATTY 1 cut(s) 26
AfaI GTAC 3 cut(s) 167, 185, 198
AgsI TTSAA 2 cut(s) 31, 88
AluBI AGCT 2 cut(s) 18, 134
AluI AGCT 2 cut(s) 18, 134
ApeKI GCWGC 2 cut(s) 18, 192
ApoI RAATTY 1 cut(s) 26
Asp718I GGTACC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 183
BbvI GCAGC 2 cut(s) 5, 204
BfaI CTAG 1 cut(s) 313
BisI GCNGC 3 cut(s) 19, 52, 193
BlsI GCNGC 3 cut(s) 20, 53, 194
BmiI GGNNCC 1 cut(s) 185
BmsI GCATC 1 cut(s) 164
BpmI CTGGAG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 65, 187
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 65, 187
BseXI GCAGC 2 cut(s) 5, 204
BsgI GTGCAG 1 cut(s) 211
Bsh1285I CGRYCG 1 cut(s) 100
BshNI GGYRCC 1 cut(s) 183
BshVI ATCGAT 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 100
Bsp143I GATC 2 cut(s) 12, 268
BspACI CCGC 2 cut(s) 51, 207
BspDI ATCGAT 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 183
BsrI ACTGG 2 cut(s) 65, 187
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 2 cut(s) 12, 268
BssT1I CCWWGG 1 cut(s) 178
Bst4CI ACNGT 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 283
BstF5I GGATG 1 cut(s) 168
BstKTI GATC 2 cut(s) 15, 271
BstMBI GATC 2 cut(s) 12, 268
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 5, 204
Bsu15I ATCGAT 1 cut(s) 11
BsuTUI ATCGAT 1 cut(s) 11
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 1 cut(s) 168
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 283
ClaI ATCGAT 1 cut(s) 11
Csp6I GTAC 3 cut(s) 166, 184, 197
CviJI RGCY 5 cut(s) 18, 54, 94, 134, 245
CviKI_1 RGCY 5 cut(s) 18, 54, 94, 134, 245
CviQI GTAC 3 cut(s) 166, 184, 197
DpnI GATC 2 cut(s) 14, 270
DpnII GATC 2 cut(s) 12, 268
Eco130I CCWWGG 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 178
FaiI YATR 3 cut(s) 140, 249, 274
Fnu4HI GCNGC 3 cut(s) 19, 52, 193
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 3 cut(s) 19, 52, 193
FspBI CTAG 1 cut(s) 313
GluI GCNGC 3 cut(s) 19, 52, 193
GsuI CTGGAG 1 cut(s) 26
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 1 cut(s) 173
Hpy8I GTNNAC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 1 cut(s) 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
KpnI GGTACC 1 cut(s) 187
Kzo9I GATC 2 cut(s) 12, 268
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 5 cut(s) 46, 56, 200, 291, 295
Lsp1109I GCAGC 2 cut(s) 5, 204
LweI GCATC 1 cut(s) 164
MaeI CTAG 1 cut(s) 313
MalI GATC 2 cut(s) 14, 270
MboI GATC 2 cut(s) 12, 268
MboII GAAGA 1 cut(s) 76
MfeI CAATTG 1 cut(s) 292
MluCI AATT 3 cut(s) 26, 292, 298
MnlI CCTC 4 cut(s) 50, 156, 212, 235
MseI TTAA 2 cut(s) 69, 156
MspA1I CMGCKG 1 cut(s) 18
MunI CAATTG 1 cut(s) 292
MwoI GCNNNNNNNGC 1 cut(s) 231
NdeII GATC 2 cut(s) 12, 268
NlaIV GGNNCC 1 cut(s) 185
PkrI GCNGC 3 cut(s) 20, 53, 194
PspN4I GGNNCC 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 3 cut(s) 167, 185, 198
RsaNI GTAC 3 cut(s) 166, 184, 197
SaqAI TTAA 2 cut(s) 69, 156
SatI GCNGC 3 cut(s) 19, 52, 193
Sau3AI GATC 2 cut(s) 12, 268
SetI ASST 5 cut(s) 20, 136, 167, 204, 266
SfaNI GCATC 1 cut(s) 164
Sse9I AATT 3 cut(s) 26, 292, 298
SsiI CCGC 2 cut(s) 51, 207
SspMI CTAG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 2 cut(s) 11, 96
TasI AATT 3 cut(s) 26, 292, 298
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 2 cut(s) 69, 156
Tru9I TTAA 2 cut(s) 69, 156
TscAI CASTG 1 cut(s) 194
TseI GCWGC 2 cut(s) 18, 192
TspRI CASTG 1 cut(s) 194
XapI RAATTY 1 cut(s) 26
XspI CTAG 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.