Rh4CG065900

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000286
Physical Location & Seq
Forward (+)
12462170 .. 12465033
2864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG065900.1

Sequence Viewer

Length: 309 bp
ATGGCACATGCAATAAGCATCAGGGAATTCAAGAAGAGTATGGGAAGCTATTTCGACGATGACTCCGTGATGATGAAGAGAAGCTTCAAGGAGCTGGACAAGAACGGAGACGGCAAGTTGGATTTCAACGAATACAACTCTCTTTACTATCTTGTGGAGAGCGGATCACAGAGAGTGCAAGTTAATCGATCTGGATCAAAGAGGAGGGCAGTATTTGAAGCAATCAAATCTGGAGTGAGCGTTGCACATGCAGTAGGAAATGTTGCTTCCGATGTTGCATCGTGCAGCATCATCATTGTGATGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

102

Amino Acids

11.29

Weight (kDa)

8.76

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 25 - 47 5.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 162
AciI CCGC 1 cut(s) 162
AclWI GGATC 2 cut(s) 172, 202
AcsI RAATTY 1 cut(s) 26
AgsI TTSAA 4 cut(s) 31, 88, 127, 218
AluBI AGCT 3 cut(s) 48, 84, 94
AluI AGCT 3 cut(s) 48, 84, 94
Alw26I GTCTC 1 cut(s) 102
AlwI GGATC 2 cut(s) 172, 202
ApeKI GCWGC 1 cut(s) 285
ApoI RAATTY 1 cut(s) 26
BbvI GCAGC 1 cut(s) 297
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BcoDI GTCTC 1 cut(s) 102
BisI GCNGC 1 cut(s) 286
BlsI GCNGC 1 cut(s) 287
BmsI GCATC 3 cut(s) 27, 287, 297
BpmI CTGGAG 1 cut(s) 252
Bsa29I ATCGAT 1 cut(s) 187
BsaBI GATNNNNATC 1 cut(s) 193
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 225, 255
Bse8I GATNNNNATC 1 cut(s) 193
BseCI ATCGAT 1 cut(s) 187
BseJI GATNNNNATC 1 cut(s) 193
BseRI GAGGAG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 297
BsgI GTGCAG 1 cut(s) 304
BshVI ATCGAT 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 102
BsmBI CGTCTC 1 cut(s) 102
Bsp143I GATC 3 cut(s) 164, 188, 194
BspACI CCGC 1 cut(s) 162
BspDI ATCGAT 1 cut(s) 187
BspPI GGATC 2 cut(s) 172, 202
BsrBI CCGCTC 1 cut(s) 162
BssMI GATC 3 cut(s) 164, 188, 194
Bst6I CTCTTC 2 cut(s) 29, 71
BstKTI GATC 3 cut(s) 167, 191, 197
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 3 cut(s) 164, 188, 194
BstNSI RCATGY 2 cut(s) 11, 251
BstV1I GCAGC 1 cut(s) 297
Bsu15I ATCGAT 1 cut(s) 187
BsuTUI ATCGAT 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 187
CviAII CATG 2 cut(s) 8, 248
CviJI RGCY 3 cut(s) 48, 84, 94
CviKI_1 RGCY 3 cut(s) 48, 84, 94
DpnI GATC 3 cut(s) 166, 190, 196
DpnII GATC 3 cut(s) 164, 188, 194
Eam1104I CTCTTC 2 cut(s) 29, 71
EarI CTCTTC 2 cut(s) 29, 71
EcoRI GAATTC 1 cut(s) 26
Esp3I CGTCTC 1 cut(s) 102
FaeI CATG 2 cut(s) 11, 251
FaiI YATR 4 cut(s) 9, 41, 249, 307
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FatI CATG 2 cut(s) 7, 247
Fnu4HI GCNGC 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 286
GluI GCNGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 252
Hin1II CATG 2 cut(s) 11, 251
HindIII AAGCTT 1 cut(s) 82
HinfI GANTC 1 cut(s) 62
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 3 cut(s) 31, 192, 231
Hpy99I CGWCG 1 cut(s) 59
HpyCH4V TGCA 6 cut(s) 11, 178, 245, 251, 278, 285
Hsp92II CATG 2 cut(s) 11, 251
Kzo9I GATC 3 cut(s) 164, 188, 194
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 4 cut(s) 7, 80, 177, 216
Lsp1109I GCAGC 1 cut(s) 297
LweI GCATC 3 cut(s) 27, 287, 297
MalI GATC 3 cut(s) 166, 190, 196
MbiI CCGCTC 1 cut(s) 162
MboI GATC 3 cut(s) 164, 188, 194
MboII GAAGA 2 cut(s) 46, 88
MluCI AATT 1 cut(s) 26
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 2 cut(s) 195, 198
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 2 cut(s) 296, 299
NdeII GATC 3 cut(s) 164, 188, 194
NlaIII CATG 2 cut(s) 11, 251
NspI RCATGY 2 cut(s) 11, 251
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PkrI GCNGC 1 cut(s) 287
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
RseI CAYNNNNRTG 2 cut(s) 296, 299
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 3 cut(s) 164, 188, 194
SchI GAGTC 1 cut(s) 56
SetI ASST 3 cut(s) 50, 86, 96
SfaNI GCATC 3 cut(s) 27, 287, 297
SmiMI CAYNNNNRTG 2 cut(s) 296, 299
Sse9I AATT 1 cut(s) 26
SsiI CCGC 1 cut(s) 162
TaqI TCGA 2 cut(s) 54, 187
TasI AATT 1 cut(s) 26
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseI GCWGC 1 cut(s) 285
TspDTI ATGAA 1 cut(s) 89
TspGWI ACGGA 2 cut(s) 55, 120
XapI RAATTY 1 cut(s) 26
XceI RCATGY 2 cut(s) 11, 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.