Rh4CG068000

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000286
Physical Location & Seq
Forward (+)
12726539 .. 12728866
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG068000.1

Sequence Viewer

Length: 1152 bp
ATGGAAGAAATACGTGAAGTTGCTTTAGCATACTATGACAATTGCACACCAGAACTTCGGGGTAAGACATGGGAGTTCTTTCAATCCATGGATGGGAATGGCGATCACCGAATCAGTCCCAGGGAGTTCAATGAATTCCTCCGTCAGACCGGTTACAACTCGATCATCACTGACCCAGAATTCTTTGCAAAGCTTGACCGCAACGGTGACTCCGGGCTGGATTTCGATGAAGTACTCACTTTCTACTATATCATTAAAACTGGGTACATCTGGTGCCGAGGCTGCGGCATGTACCTTTCCGGGCTCTATTTTACGTGCTTCGAATGCTTTGAGGCAGCCAATCACTCCACTTTTGATCTATGTGATGCTTGTCATCGCAGTCGGAAGTTTACTCACCACCACACACTCTTCTTGGATAACCATATATTGCTATACTACAAAAGAGGACCAGATGACAGCATGCCAATGGAAAATCTATATGCAACAGCTATAGCAGATTATTACAACGCCGCTCAAGAGCATCGGAGTTCAGCGGAGTCTTTCTTTCACTCCATGGCTATACATGGTGCAAACCAAATCAGCACCACTGTGTTCAACGATTTCCTCGCGAAAAAAGGCTACAACTGGATTATTAATGATCCACTCTTGTTCCAGAAACTCGACCGCAACCGTGATGGCAGCCTCGATTTCTGGGAATTGCTCACCTTCTACTACATCATTAGCACAAGGTACTTCCATTGCCGAGAATGTCAGGTGTACCTTGGCGGGCTGTATTTTACCTGCGTTGCTTGCTTTGAGGAAGGAGCTCATTATCGTAGTCATCATCATTCTGATCCCGGCACCTATAATCTATGTGCTGCCTGCTATCGCAGTAGGAATTTCGTCTCTCAACACCCGCACCACACCTTCTTCTTGGATAACCACATATTGCTGCGCTCCAAAAGGGGTCCTCCTCCTTATGCTTCACCAAGTCTGGTAAGCACTCCAAAACCTCTGGTGGTTTATAATACTTATTATTTTATTAATAATAATTACCACCTTCCAGCTGTGCAGCCTGTACCTCCAAAGCAAACTTGGCTCTCGGCACTTAACGACCTTTTAGAGGTGGCTCTTGCTGGTGCAAACTTGGCAGTTAATTGTTCAATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

383

Amino Acids

44.43

Weight (kDa)

6.03

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 83 1.9e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 2 cut(s) 273, 839
AccBSI CCGCTC 1 cut(s) 512
AccII CGCG 1 cut(s) 608
AciI CCGC 7 cut(s) 199, 285, 510, 533, 664, 765, 896
AclWI GGATC 2 cut(s) 632, 827
AcsI RAATTY 3 cut(s) 134, 179, 877
AfaI GTAC 6 cut(s) 234, 266, 293, 731, 758, 1059
AfiI CCNNNNNNNGG 2 cut(s) 93, 1102
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 4 cut(s) 83, 130, 595, 1143
AjnI CCWGG 1 cut(s) 119
AleI CACNNNNGTG 1 cut(s) 587
AluBI AGCT 4 cut(s) 193, 488, 806, 1046
AluI AGCT 4 cut(s) 193, 488, 806, 1046
Alw21I GWGCWC 1 cut(s) 808
Alw26I GTCTC 1 cut(s) 889
AlwI GGATC 2 cut(s) 632, 827
ApeKI GCWGC 6 cut(s) 282, 335, 678, 857, 931, 1051
ApoI RAATTY 3 cut(s) 134, 179, 877
AseI ATTAAT 2 cut(s) 633, 1023
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 1 cut(s) 936
AspS9I GGNCC 2 cut(s) 446, 947
AsuC2I CCSGG 3 cut(s) 214, 301, 837
AsuHPI GGTGA 5 cut(s) 98, 218, 386, 694, 957
AsuII TTCGAA 1 cut(s) 321
AvaII GGWCC 2 cut(s) 446, 947
BanI GGYRCC 2 cut(s) 273, 839
BanII GRGCYC 2 cut(s) 306, 808
Bbv12I GWGCWC 1 cut(s) 808
BbvI GCAGC 6 cut(s) 269, 347, 690, 844, 918, 1063
BccI CCATC 2 cut(s) 86, 668
BciT130I CCWGG 1 cut(s) 121
BcnI CCSGG 3 cut(s) 214, 301, 837
BcoDI GTCTC 1 cut(s) 889
BfmI CTRYAG 1 cut(s) 489
BfuAI ACCTGC 1 cut(s) 788
BisI GCNGC 8 cut(s) 283, 286, 336, 510, 679, 858, 932, 1052
BlsI GCNGC 8 cut(s) 284, 287, 337, 511, 680, 859, 933, 1053
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 4 cut(s) 121, 214, 301, 837
Bme18I GGWCC 2 cut(s) 446, 947
BmgT120I GGNCC 2 cut(s) 446, 947
BmiI GGNNCC 3 cut(s) 275, 841, 948
BmrFI CCNGG 4 cut(s) 121, 214, 301, 837
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 355, 529
BmuI ACTGGG 1 cut(s) 270
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 498
BpuMI CCSGG 3 cut(s) 214, 301, 837
BsaAI YACGTR 2 cut(s) 14, 315
BsaJI CCNNGG 6 cut(s) 87, 119, 120, 277, 552, 760
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 1102
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 265, 629
Bse3DI GCAATG 1 cut(s) 736
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 6 cut(s) 87, 119, 120, 277, 552, 760
BseGI GGATG 1 cut(s) 97
BseLI CCNNNNNNNGG 2 cut(s) 93, 1102
BseMI GCAATG 1 cut(s) 736
BseNI ACTGG 2 cut(s) 265, 629
BseRI GAGGAG 1 cut(s) 942
BseXI GCAGC 6 cut(s) 269, 347, 690, 844, 918, 1063
BsgI GTGCAG 1 cut(s) 1070
Bsh1236I CGCG 1 cut(s) 608
Bsh1285I CGRYCG 1 cut(s) 664
BshNI GGYRCC 2 cut(s) 273, 839
BshTI ACCGGT 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 1 cut(s) 808
BsiSI CCGG 4 cut(s) 150, 213, 300, 837
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 2 cut(s) 93, 1102
BsmAI GTCTC 1 cut(s) 889
BsmBI CGTCTC 1 cut(s) 889
BsmFI GGGAC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 306, 808
Bsp143I GATC 5 cut(s) 103, 162, 355, 637, 832
Bsp19I CCATGG 2 cut(s) 87, 552
Bsp68I TCGCGA 1 cut(s) 608
BspACI CCGC 7 cut(s) 199, 285, 510, 533, 664, 765, 896
BspFNI CGCG 1 cut(s) 608
BspLI GGNNCC 3 cut(s) 275, 841, 948
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 2 cut(s) 632, 827
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 2 cut(s) 273, 839
BsrBI CCGCTC 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 2 cut(s) 265, 629
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 6 cut(s) 87, 119, 120, 277, 552, 760
BssMI GATC 5 cut(s) 103, 162, 355, 637, 832
BssT1I CCWWGG 3 cut(s) 87, 552, 760
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 3 cut(s) 206, 589, 671
Bst6I CTCTTC 1 cut(s) 413
BstBAI YACGTR 2 cut(s) 14, 315
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 4 cut(s) 461, 767, 790, 862
BstDSI CCRYGG 2 cut(s) 87, 552
BstENI CCTNNNNNAGG 1 cut(s) 1100
BstF5I GGATG 1 cut(s) 97
BstFNI CGCG 1 cut(s) 608
BstHHI GCGC 1 cut(s) 936
BstKTI GATC 5 cut(s) 106, 165, 358, 640, 835
BstMAI GTCTC 1 cut(s) 889
BstMBI GATC 5 cut(s) 103, 162, 355, 637, 832
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 5 cut(s) 282, 324, 789, 1075, 1127
BstNI CCWGG 1 cut(s) 121
BstNSI RCATGY 2 cut(s) 292, 463
BstSCI CCNGG 4 cut(s) 119, 212, 299, 835
BstSFI CTRYAG 1 cut(s) 489
BstUI CGCG 1 cut(s) 608
BstV1I GCAGC 6 cut(s) 269, 347, 690, 844, 918, 1063
BtgI CCRYGG 2 cut(s) 87, 552
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 1 cut(s) 97
BtsIMutI CAGTG 2 cut(s) 168, 585
BtuMI TCGCGA 1 cut(s) 608
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 4 cut(s) 461, 767, 790, 862
CfoI GCGC 1 cut(s) 936
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 446, 947
Csp6I GTAC 6 cut(s) 233, 265, 292, 730, 757, 1058
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 6 cut(s) 69, 88, 289, 460, 553, 563
CviQI GTAC 6 cut(s) 233, 265, 292, 730, 757, 1058
DpnI GATC 5 cut(s) 105, 164, 357, 639, 834
DpnII GATC 5 cut(s) 103, 162, 355, 637, 832
Eam1104I CTCTTC 1 cut(s) 413
EarI CTCTTC 1 cut(s) 413
Ecl136II GAGCTC 1 cut(s) 806
Eco130I CCWWGG 3 cut(s) 87, 552, 760
Eco24I GRGCYC 2 cut(s) 306, 808
Eco47I GGWCC 2 cut(s) 446, 947
Eco53kI GAGCTC 1 cut(s) 806
EcoICRI GAGCTC 1 cut(s) 806
EcoNI CCTNNNNNAGG 1 cut(s) 1100
EcoO109I RGGNCCY 1 cut(s) 947
EcoRI GAATTC 2 cut(s) 134, 179
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 3 cut(s) 87, 552, 760
EcoT38I GRGCYC 2 cut(s) 306, 808
ErhI CCWWGG 3 cut(s) 87, 552, 760
Esp3I CGTCTC 1 cut(s) 889
FaeI CATG 6 cut(s) 72, 91, 292, 463, 556, 566
FaqI GGGAC 1 cut(s) 102
FatI CATG 6 cut(s) 68, 87, 288, 459, 552, 562
FauI CCCGC 2 cut(s) 758, 903
Fnu4HI GCNGC 8 cut(s) 283, 286, 336, 510, 679, 858, 932, 1052
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 2 cut(s) 306, 808
Fsp4HI GCNGC 8 cut(s) 283, 286, 336, 510, 679, 858, 932, 1052
GlaI GCGC 1 cut(s) 935
GluI GCNGC 8 cut(s) 283, 286, 336, 510, 679, 858, 932, 1052
HapII CCGG 4 cut(s) 150, 213, 300, 837
HhaI GCGC 1 cut(s) 936
Hin1II CATG 6 cut(s) 72, 91, 292, 463, 556, 566
Hin6I GCGC 1 cut(s) 934
HinP1I GCGC 1 cut(s) 934
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 3 cut(s) 111, 209, 536
HpaII CCGG 4 cut(s) 150, 213, 300, 837
HphI GGTGA 5 cut(s) 98, 218, 386, 694, 957
Hpy166II GTNNAC 2 cut(s) 390, 757
Hpy188I TCNGA 4 cut(s) 147, 384, 525, 832
Hpy188III TCNNGA 3 cut(s) 515, 607, 652
Hpy8I GTNNAC 2 cut(s) 390, 757
HpyAV CCTTC 4 cut(s) 715, 794, 916, 1049
HpyCH4III ACNGT 3 cut(s) 206, 589, 671
HpyCH4IV ACGT 2 cut(s) 13, 314
HpyCH4V TGCA 6 cut(s) 45, 188, 482, 569, 1051, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 324, 789, 1075, 1127
HpySE526I ACGT 2 cut(s) 13, 314
Hsp92II CATG 6 cut(s) 72, 91, 292, 463, 556, 566
HspAI GCGC 1 cut(s) 934
Kzo9I GATC 5 cut(s) 103, 162, 355, 637, 832
LmnI GCTCC 2 cut(s) 803, 941
Lsp1109I GCAGC 6 cut(s) 269, 347, 690, 844, 918, 1063
LweI GCATC 2 cut(s) 355, 529
MaeII ACGT 2 cut(s) 13, 314
MaeIII GTNAC 2 cut(s) 152, 206
MalI GATC 5 cut(s) 105, 164, 357, 639, 834
MbiI CCGCTC 1 cut(s) 512
MboI GATC 5 cut(s) 103, 162, 355, 637, 832
MboII GAAGA 3 cut(s) 17, 400, 901
MfeI CAATTG 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 306, 808
MluCI AATT 8 cut(s) 40, 134, 179, 695, 877, 1030, 1135, 1143
MlyI GAGTC 2 cut(s) 203, 545
MmeI TCCRAC 1 cut(s) 362
MseI TTAA 5 cut(s) 255, 633, 1023, 1089, 1134
MslI CAYNNNNRTG 2 cut(s) 464, 587
MspA1I CMGCKG 2 cut(s) 533, 1046
MspI CCGG 4 cut(s) 150, 213, 300, 837
MspR9I CCNGG 4 cut(s) 121, 214, 301, 837
MunI CAATTG 1 cut(s) 40
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 1 cut(s) 121
MvnI CGCG 1 cut(s) 608
MwoI GCNNNNNNNGC 5 cut(s) 282, 324, 789, 1075, 1127
NciI CCSGG 3 cut(s) 214, 301, 837
NcoI CCATGG 2 cut(s) 87, 552
NdeII GATC 5 cut(s) 103, 162, 355, 637, 832
NlaIII CATG 6 cut(s) 72, 91, 292, 463, 556, 566
NlaIV GGNNCC 3 cut(s) 275, 841, 948
NmeAIII GCCGAG 3 cut(s) 302, 767, 1061
NmuCI GTSAC 1 cut(s) 206
NruI TCGCGA 1 cut(s) 608
NspI RCATGY 2 cut(s) 292, 463
NspV TTCGAA 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 587
PaeI GCATGC 1 cut(s) 463
PasI CCCWGGG 1 cut(s) 120
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 8 cut(s) 284, 287, 337, 511, 680, 859, 933, 1053
PleI GAGTC 2 cut(s) 203, 544
PpsI GAGTC 2 cut(s) 203, 544
Ppu21I YACGTR 2 cut(s) 14, 315
PpuMI RGGWCCY 1 cut(s) 947
PshBI ATTAAT 2 cut(s) 633, 1023
PsiI TTATAA 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 808
Psp5II RGGWCCY 1 cut(s) 947
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 3 cut(s) 275, 841, 948
PspPI GGNCC 2 cut(s) 446, 947
PspPPI RGGWCCY 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 1046
RruI TCGCGA 1 cut(s) 608
RsaI GTAC 6 cut(s) 234, 266, 293, 731, 758, 1059
RsaNI GTAC 6 cut(s) 233, 265, 292, 730, 757, 1058
RseI CAYNNNNRTG 2 cut(s) 464, 587
SacI GAGCTC 1 cut(s) 808
SaqAI TTAA 5 cut(s) 255, 633, 1023, 1089, 1134
SatI GCNGC 8 cut(s) 283, 286, 336, 510, 679, 858, 932, 1052
Sau3AI GATC 5 cut(s) 103, 162, 355, 637, 832
Sau96I GGNCC 2 cut(s) 446, 947
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 2 cut(s) 203, 545
ScrFI CCNGG 4 cut(s) 121, 214, 301, 837
SduI GDGCHC 2 cut(s) 306, 808
SfaNI GCATC 2 cut(s) 355, 529
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 321
SinI GGWCC 2 cut(s) 446, 947
SmiMI CAYNNNNRTG 2 cut(s) 464, 587
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
SphI GCATGC 1 cut(s) 463
Sse9I AATT 8 cut(s) 40, 134, 179, 695, 877, 1030, 1135, 1143
SsiI CCGC 7 cut(s) 199, 285, 510, 533, 664, 765, 896
SstI GAGCTC 1 cut(s) 808
StyD4I CCNGG 4 cut(s) 119, 212, 299, 835
StyI CCWWGG 3 cut(s) 87, 552, 760
TaaI ACNGT 3 cut(s) 206, 589, 671
TaiI ACGT 2 cut(s) 16, 317
TaqI TCGA 5 cut(s) 161, 225, 321, 660, 684
TasI AATT 8 cut(s) 40, 134, 179, 695, 877, 1030, 1135, 1143
TatI WGTACW 1 cut(s) 232
TauI GCSGC 2 cut(s) 288, 512
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 5 cut(s) 255, 633, 1023, 1089, 1134
Tru9I TTAA 5 cut(s) 255, 633, 1023, 1089, 1134
TscAI CASTG 2 cut(s) 175, 592
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 6 cut(s) 282, 335, 678, 857, 931, 1051
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 2 cut(s) 147, 243
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 2 cut(s) 175, 592
VpaK11BI GGWCC 2 cut(s) 446, 947
VspI ATTAAT 2 cut(s) 633, 1023
XagI CCTNNNNNAGG 1 cut(s) 1100
XapI RAATTY 3 cut(s) 134, 179, 877
XceI RCATGY 2 cut(s) 292, 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 1071
ZrmI AGTACT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.