EF hand
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1176 |
| AccB1I | GGYRCC | 1 cut(s) | 284 |
| AccBSI | CCGCTC | 1 cut(s) | 171 |
| AccII | CGCG | 1 cut(s) | 927 |
| AccIII | TCCGGA | 2 cut(s) | 598, 1218 |
| AclI | AACGTT | 1 cut(s) | 899 |
| AclWI | GGATC | 7 cut(s) | 80, 272, 452, 596, 609, 1229, 1251 |
| AcoI | YGGCCR | 1 cut(s) | 817 |
| AcsI | RAATTY | 2 cut(s) | 322, 775 |
| AcyI | GRCGYC | 1 cut(s) | 1173 |
| AdeI | CACNNNGTG | 1 cut(s) | 14 |
| AfaI | GTAC | 2 cut(s) | 179, 1037 |
| AfeI | AGCGCT | 1 cut(s) | 724 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 460, 564, 589, 1076, 1261 |
| AflII | CTTAAG | 1 cut(s) | 536 |
| AgsI | TTSAA | 8 cut(s) | 43, 184, 328, 732, 771, 1188, 1268, 1279 |
| AjnI | CCWGG | 1 cut(s) | 563 |
| AluBI | AGCT | 8 cut(s) | 30, 254, 710, 893, 957, 1042, 1097, 1184 |
| AluI | AGCT | 8 cut(s) | 30, 254, 710, 893, 957, 1042, 1097, 1184 |
| Alw21I | GWGCWC | 1 cut(s) | 256 |
| Alw26I | GTCTC | 2 cut(s) | 544, 746 |
| AlwI | GGATC | 7 cut(s) | 80, 272, 452, 596, 609, 1229, 1251 |
| Aor13HI | TCCGGA | 2 cut(s) | 598, 1218 |
| Aor51HI | AGCGCT | 1 cut(s) | 724 |
| AoxI | GGCC | 4 cut(s) | 450, 566, 817, 831 |
| ApeKI | GCWGC | 9 cut(s) | 114, 126, 302, 707, 725, 893, 979, 992, 1212 |
| ApoI | RAATTY | 2 cut(s) | 322, 775 |
| AseI | ATTAAT | 1 cut(s) | 81 |
| AspLEI | GCGC | 1 cut(s) | 725 |
| AspS9I | GGNCC | 1 cut(s) | 451 |
| AsuC2I | CCSGG | 2 cut(s) | 282, 590 |
| AsuHPI | GGTGA | 3 cut(s) | 142, 1091, 1096 |
| BalI | TGGCCA | 1 cut(s) | 819 |
| BamHI | GGATCC | 1 cut(s) | 601 |
| BanI | GGYRCC | 1 cut(s) | 284 |
| BanII | GRGCYC | 2 cut(s) | 256, 614 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 212, 244 |
| BauI | CACGAG | 1 cut(s) | 785 |
| Bbv12I | GWGCWC | 1 cut(s) | 256 |
| BbvI | GCAGC | 9 cut(s) | 126, 138, 289, 712, 719, 880, 966, 979, 1224 |
| BccI | CCATC | 5 cut(s) | 239, 273, 682, 809, 923 |
| BceAI | ACGGC | 3 cut(s) | 479, 615, 771 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 875, 909 |
| BciT130I | CCWGG | 1 cut(s) | 565 |
| BclI | TGATCA | 1 cut(s) | 1024 |
| BcnI | CCSGG | 2 cut(s) | 282, 590 |
| BcoDI | GTCTC | 2 cut(s) | 544, 746 |
| BfaI | CTAG | 1 cut(s) | 1119 |
| BfmI | CTRYAG | 1 cut(s) | 489 |
| BfoI | RGCGCY | 1 cut(s) | 726 |
| BfrI | CTTAAG | 1 cut(s) | 536 |
| Bme1390I | CCNGG | 3 cut(s) | 282, 565, 590 |
| BmgT120I | GGNCC | 1 cut(s) | 451 |
| BmiI | GGNNCC | 4 cut(s) | 286, 594, 603, 652 |
| BmrFI | CCNGG | 3 cut(s) | 282, 565, 590 |
| Bpu10I | CCTNAGC | 1 cut(s) | 209 |
| BpuEI | CTTGAG | 2 cut(s) | 157, 1069 |
| BpuMI | CCSGG | 2 cut(s) | 282, 590 |
| Bsa29I | ATCGAT | 1 cut(s) | 1246 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1026 |
| BsaHI | GRCGYC | 1 cut(s) | 1173 |
| BsaJI | CCNNGG | 6 cut(s) | 350, 563, 820, 987, 1108, 1196 |
| BsaWI | WCCGGW | 3 cut(s) | 598, 748, 1218 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 331, 361 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 460, 564, 589, 1076, 1261 |
| Bse1I | ACTGG | 1 cut(s) | 743 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1026 |
| BseAI | TCCGGA | 2 cut(s) | 598, 1218 |
| BseBI | CCWGG | 1 cut(s) | 565 |
| BseCI | ATCGAT | 1 cut(s) | 1246 |
| BseDI | CCNNGG | 6 cut(s) | 350, 563, 820, 987, 1108, 1196 |
| BseGI | GGATG | 3 cut(s) | 165, 265, 830 |
| BseJI | GATNNNNATC | 1 cut(s) | 1026 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 460, 564, 589, 1076, 1261 |
| BseNI | ACTGG | 1 cut(s) | 743 |
| BseRI | GAGGAG | 1 cut(s) | 264 |
| BseXI | GCAGC | 9 cut(s) | 126, 138, 289, 712, 719, 880, 966, 979, 1224 |
| BsgI | GTGCAG | 2 cut(s) | 36, 895 |
| Bsh1236I | CGCG | 1 cut(s) | 927 |
| BshFI | GGCC | 4 cut(s) | 452, 568, 819, 833 |
| BshNI | GGYRCC | 1 cut(s) | 284 |
| BshVI | ATCGAT | 1 cut(s) | 1246 |
| BsiHKAI | GWGCWC | 1 cut(s) | 256 |
| BsiSI | CCGG | 7 cut(s) | 282, 461, 589, 599, 693, 749, 1219 |
| BslFI | GGGAC | 1 cut(s) | 1215 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 460, 564, 589, 1076, 1261 |
| BsmAI | GTCTC | 2 cut(s) | 544, 746 |
| BsmBI | CGTCTC | 1 cut(s) | 746 |
| BsmFI | GGGAC | 1 cut(s) | 1215 |
| BsnI | GGCC | 4 cut(s) | 452, 568, 819, 833 |
| Bsp1286I | GDGCHC | 2 cut(s) | 256, 614 |
| Bsp13I | TCCGGA | 2 cut(s) | 598, 1218 |
| Bsp19I | CCATGG | 3 cut(s) | 820, 1108, 1196 |
| BspANI | GGCC | 4 cut(s) | 452, 568, 819, 833 |
| BspDI | ATCGAT | 1 cut(s) | 1246 |
| BspEI | TCCGGA | 2 cut(s) | 598, 1218 |
| BspFNI | CGCG | 1 cut(s) | 927 |
| BspHI | TCATGA | 1 cut(s) | 1030 |
| BspLI | GGNNCC | 4 cut(s) | 286, 594, 603, 652 |
| BspPI | GGATC | 7 cut(s) | 80, 272, 452, 596, 609, 1229, 1251 |
| BspT107I | GGYRCC | 1 cut(s) | 284 |
| BspTI | CTTAAG | 1 cut(s) | 536 |
| BsrBI | CCGCTC | 1 cut(s) | 171 |
| BsrI | ACTGG | 1 cut(s) | 743 |
| BssECI | CCNNGG | 6 cut(s) | 350, 563, 820, 987, 1108, 1196 |
| BssNI | GRCGYC | 1 cut(s) | 1173 |
| BssSI | CACGAG | 1 cut(s) | 785 |
| BssT1I | CCWWGG | 5 cut(s) | 350, 820, 987, 1108, 1196 |
| Bst2BI | CACGAG | 1 cut(s) | 785 |
| Bst2UI | CCWGG | 1 cut(s) | 565 |
| Bst4CI | ACNGT | 3 cut(s) | 37, 855, 1079 |
| Bst6I | CTCTTC | 2 cut(s) | 542, 569 |
| BstACI | GRCGYC | 1 cut(s) | 1173 |
| BstAFI | CTTAAG | 1 cut(s) | 536 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 918 |
| BstC8I | GCNNGC | 3 cut(s) | 307, 955, 1055 |
| BstDEI | CTNAG | 2 cut(s) | 209, 1065 |
| BstDSI | CCRYGG | 3 cut(s) | 820, 1108, 1196 |
| BstF5I | GGATG | 3 cut(s) | 165, 265, 830 |
| BstFNI | CGCG | 1 cut(s) | 927 |
| BstH2I | RGCGCY | 1 cut(s) | 726 |
| BstHHI | GCGC | 1 cut(s) | 725 |
| BstMAI | GTCTC | 2 cut(s) | 544, 746 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 123, 873, 918 |
| BstNI | CCWGG | 1 cut(s) | 565 |
| BstSCI | CCNGG | 3 cut(s) | 280, 563, 588 |
| BstSFI | CTRYAG | 1 cut(s) | 489 |
| BstUI | CGCG | 1 cut(s) | 927 |
| BstV1I | GCAGC | 9 cut(s) | 126, 138, 289, 712, 719, 880, 966, 979, 1224 |
| BstX2I | RGATCY | 1 cut(s) | 601 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 961 |
| BstYI | RGATCY | 1 cut(s) | 601 |
| Bsu15I | ATCGAT | 1 cut(s) | 1246 |
| BsuRI | GGCC | 4 cut(s) | 452, 568, 819, 833 |
| BsuTUI | ATCGAT | 1 cut(s) | 1246 |
| BtgI | CCRYGG | 3 cut(s) | 820, 1108, 1196 |
| BtgZI | GCGATG | 1 cut(s) | 942 |
| BtsCI | GGATG | 3 cut(s) | 165, 265, 830 |
| BtsI | GCAGTG | 1 cut(s) | 522 |
| BtsIMutI | CAGTG | 2 cut(s) | 33, 522 |
| Cac8I | GCNNGC | 3 cut(s) | 307, 955, 1055 |
| CciI | TCATGA | 1 cut(s) | 1030 |
| CfoI | GCGC | 1 cut(s) | 725 |
| Cfr13I | GGNCC | 1 cut(s) | 451 |
| ClaI | ATCGAT | 1 cut(s) | 1246 |
| CseI | GACGC | 1 cut(s) | 516 |
| Csp6I | GTAC | 2 cut(s) | 178, 1036 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1213, 1248 |
| CviAII | CATG | 8 cut(s) | 455, 647, 821, 950, 1031, 1109, 1138, 1197 |
| CviQI | GTAC | 2 cut(s) | 178, 1036 |
| DdeI | CTNAG | 2 cut(s) | 209, 1065 |
| DraIII | CACNNNGTG | 1 cut(s) | 14 |
| EaeI | YGGCCR | 1 cut(s) | 817 |
| Eam1104I | CTCTTC | 2 cut(s) | 542, 569 |
| EarI | CTCTTC | 2 cut(s) | 542, 569 |
| EciI | GGCGGA | 1 cut(s) | 711 |
| Ecl136II | GAGCTC | 1 cut(s) | 254 |
| Eco130I | CCWWGG | 5 cut(s) | 350, 820, 987, 1108, 1196 |
| Eco147I | AGGCCT | 1 cut(s) | 833 |
| Eco24I | GRGCYC | 2 cut(s) | 256, 614 |
| Eco47III | AGCGCT | 1 cut(s) | 724 |
| Eco53kI | GAGCTC | 1 cut(s) | 254 |
| EcoICRI | GAGCTC | 1 cut(s) | 254 |
| EcoRI | GAATTC | 1 cut(s) | 775 |
| EcoRII | CCWGG | 1 cut(s) | 563 |
| EcoT14I | CCWWGG | 5 cut(s) | 350, 820, 987, 1108, 1196 |
| EcoT38I | GRGCYC | 2 cut(s) | 256, 614 |
| ErhI | CCWWGG | 5 cut(s) | 350, 820, 987, 1108, 1196 |
| Esp3I | CGTCTC | 1 cut(s) | 746 |
| FaeI | CATG | 8 cut(s) | 458, 650, 824, 953, 1034, 1112, 1141, 1200 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 712, 744 |
| FaqI | GGGAC | 1 cut(s) | 1215 |
| FatI | CATG | 8 cut(s) | 454, 646, 820, 949, 1030, 1108, 1137, 1196 |
| FauI | CCCGC | 2 cut(s) | 206, 635 |
| FbaI | TGATCA | 1 cut(s) | 1024 |
| FokI | GGATG | 3 cut(s) | 152, 252, 837 |
| FriOI | GRGCYC | 2 cut(s) | 256, 614 |
| FspBI | CTAG | 1 cut(s) | 1119 |
| GlaI | GCGC | 1 cut(s) | 724 |
| HaeII | RGCGCY | 1 cut(s) | 726 |
| HaeIII | GGCC | 4 cut(s) | 452, 568, 819, 833 |
| HapII | CCGG | 7 cut(s) | 282, 461, 589, 599, 693, 749, 1219 |
| HgaI | GACGC | 1 cut(s) | 516 |
| HhaI | GCGC | 1 cut(s) | 725 |
| Hin1I | GRCGYC | 1 cut(s) | 1173 |
| Hin1II | CATG | 8 cut(s) | 458, 650, 824, 953, 1034, 1112, 1141, 1200 |
| Hin6I | GCGC | 1 cut(s) | 723 |
| HinP1I | GCGC | 1 cut(s) | 723 |
| HindIII | AAGCTT | 1 cut(s) | 1182 |
| HinfI | GANTC | 3 cut(s) | 1001, 1134, 1248 |
| HpaII | CCGG | 7 cut(s) | 282, 461, 589, 599, 693, 749, 1219 |
| HphI | GGTGA | 3 cut(s) | 142, 1091, 1096 |
| Hpy166II | GTNNAC | 2 cut(s) | 150, 1082 |
| Hpy188I | TCNGA | 5 cut(s) | 277, 714, 1088, 1147, 1171 |
| Hpy188III | TCNNGA | 3 cut(s) | 599, 1031, 1219 |
| Hpy8I | GTNNAC | 2 cut(s) | 150, 1082 |
| Hpy99I | CGWCG | 7 cut(s) | 500, 532, 535, 640, 890, 1175, 1178 |
| HpyAV | CCTTC | 4 cut(s) | 163, 238, 624, 1116 |
| HpyCH4III | ACNGT | 3 cut(s) | 37, 855, 1079 |
| HpyCH4IV | ACGT | 4 cut(s) | 663, 899, 972, 1173 |
| HpyCH4V | TGCA | 3 cut(s) | 17, 896, 912 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 123, 873, 918 |
| HpyF3I | CTNAG | 2 cut(s) | 209, 1065 |
| HpySE526I | ACGT | 4 cut(s) | 663, 899, 972, 1173 |
| Hsp92I | GRCGYC | 1 cut(s) | 1173 |
| Hsp92II | CATG | 8 cut(s) | 458, 650, 824, 953, 1034, 1112, 1141, 1200 |
| HspAI | GCGC | 1 cut(s) | 723 |
| Kpn2I | TCCGGA | 2 cut(s) | 598, 1218 |
| Ksp22I | TGATCA | 1 cut(s) | 1024 |
| LmnI | GCTCC | 4 cut(s) | 251, 379, 650, 715 |
| Lsp1109I | GCAGC | 9 cut(s) | 126, 138, 289, 712, 719, 880, 966, 979, 1224 |
| MaeI | CTAG | 1 cut(s) | 1119 |
| MaeII | ACGT | 4 cut(s) | 663, 899, 972, 1173 |
| MaeIII | GTNAC | 1 cut(s) | 734 |
| MbiI | CCGCTC | 1 cut(s) | 171 |
| MboII | GAAGA | 8 cut(s) | 411, 553, 556, 559, 586, 1031, 1143, 1152 |
| MflI | RGATCY | 1 cut(s) | 601 |
| MhlI | GDGCHC | 2 cut(s) | 256, 614 |
| MlsI | TGGCCA | 1 cut(s) | 819 |
| MluCI | AATT | 6 cut(s) | 322, 391, 433, 775, 1089, 1274 |
| MluNI | TGGCCA | 1 cut(s) | 819 |
| MmeI | TCCRAC | 2 cut(s) | 743, 1194 |
| Mox20I | TGGCCA | 1 cut(s) | 819 |
| MroI | TCCGGA | 2 cut(s) | 598, 1218 |
| MscI | TGGCCA | 1 cut(s) | 819 |
| MseI | TTAA | 2 cut(s) | 81, 537 |
| MslI | CAYNNNNRTG | 2 cut(s) | 948, 1113 |
| Msp20I | TGGCCA | 1 cut(s) | 819 |
| MspA1I | CMGCKG | 1 cut(s) | 117 |
| MspCI | CTTAAG | 1 cut(s) | 536 |
| MspI | CCGG | 7 cut(s) | 282, 461, 589, 599, 693, 749, 1219 |
| MspR9I | CCNGG | 3 cut(s) | 282, 565, 590 |
| MvaI | CCWGG | 1 cut(s) | 565 |
| MvnI | CGCG | 1 cut(s) | 927 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 123, 873, 918 |
| NciI | CCSGG | 2 cut(s) | 282, 590 |
| NcoI | CCATGG | 3 cut(s) | 820, 1108, 1196 |
| NlaIII | CATG | 8 cut(s) | 458, 650, 824, 953, 1034, 1112, 1141, 1200 |
| NlaIV | GGNNCC | 4 cut(s) | 286, 594, 603, 652 |
| NmeAIII | GCCGAG | 1 cut(s) | 215 |
| PagI | TCATGA | 1 cut(s) | 1030 |
| PceI | AGGCCT | 1 cut(s) | 833 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1252 |
| PfeI | GAWTC | 3 cut(s) | 1001, 1134, 1248 |
| PshBI | ATTAAT | 1 cut(s) | 81 |
| Psp124BI | GAGCTC | 1 cut(s) | 256 |
| Psp1406I | AACGTT | 1 cut(s) | 899 |
| Psp6I | CCWGG | 1 cut(s) | 563 |
| PspGI | CCWGG | 1 cut(s) | 563 |
| PspN4I | GGNNCC | 4 cut(s) | 286, 594, 603, 652 |
| PspPI | GGNCC | 1 cut(s) | 451 |
| PsuI | RGATCY | 1 cut(s) | 601 |
| RsaI | GTAC | 2 cut(s) | 179, 1037 |
| RsaNI | GTAC | 2 cut(s) | 178, 1036 |
| RseI | CAYNNNNRTG | 2 cut(s) | 948, 1113 |
| SacI | GAGCTC | 1 cut(s) | 256 |
| SaqAI | TTAA | 2 cut(s) | 81, 537 |
| Sau96I | GGNCC | 1 cut(s) | 451 |
| ScrFI | CCNGG | 3 cut(s) | 282, 565, 590 |
| SduI | GDGCHC | 2 cut(s) | 256, 614 |
| SfcI | CTRYAG | 1 cut(s) | 489 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 948, 1113 |
| SmlI | CTYRAG | 3 cut(s) | 172, 536, 1048 |
| SmoI | CTYRAG | 3 cut(s) | 172, 536, 1048 |
| Sse9I | AATT | 6 cut(s) | 322, 391, 433, 775, 1089, 1274 |
| SseBI | AGGCCT | 1 cut(s) | 833 |
| SspMI | CTAG | 1 cut(s) | 1119 |
| SstI | GAGCTC | 1 cut(s) | 256 |
| StuI | AGGCCT | 1 cut(s) | 833 |
| StyD4I | CCNGG | 3 cut(s) | 280, 563, 588 |
| StyI | CCWWGG | 5 cut(s) | 350, 820, 987, 1108, 1196 |
| TaaI | ACNGT | 3 cut(s) | 37, 855, 1079 |
| TaiI | ACGT | 4 cut(s) | 666, 902, 975, 1176 |
| TaqI | TCGA | 8 cut(s) | 132, 498, 638, 686, 903, 1044, 1246, 1272 |
| TasI | AATT | 6 cut(s) | 322, 391, 433, 775, 1089, 1274 |
| TauI | GCSGC | 1 cut(s) | 1212 |
| TfiI | GAWTC | 3 cut(s) | 1001, 1134, 1248 |
| Tru1I | TTAA | 2 cut(s) | 81, 537 |
| Tru9I | TTAA | 2 cut(s) | 81, 537 |
| TscAI | CASTG | 2 cut(s) | 40, 529 |
| TseI | GCWGC | 9 cut(s) | 114, 126, 302, 707, 725, 893, 979, 992, 1212 |
| TspDTI | ATGAA | 4 cut(s) | 587, 768, 1088, 1126 |
| TspGWI | ACGGA | 2 cut(s) | 864, 1031 |
| TspRI | CASTG | 2 cut(s) | 40, 529 |
| Vha464I | CTTAAG | 1 cut(s) | 536 |
| VspI | ATTAAT | 1 cut(s) | 81 |
| XapI | RAATTY | 2 cut(s) | 322, 775 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 661 |
| XspI | CTAG | 1 cut(s) | 1119 |
| ZraI | GACGTC | 1 cut(s) | 1174 |