Rw4G004810

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000286
Physical Location & Seq
Reverse (-)
9331531 .. 9340029
8499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004810.1

Sequence Viewer

Length: 582 bp
ATGGGAGACGTCAAAGAAATTCGTGATGCTGCTTTAGCTTATTACCAGAATTTATCTACAAAGCAACAGCAAGAGGCGTACAGTTTTTTTCAAACAATGGATAAAGATGATGATGGAACAGTAACCATGGAGGAGTTCAAGCAGATGTTTGAGGGTTATTTTGGCGACAATGTCAGCCATGCCTTCCAGGACCTAGACAAAAATGGAGATGGCAATTTGGATTTTGAGGAATCTATCACTCTTTACTACTCTCTTAAGGCTCGGAGGATCACTTTTTGCAATGGATGCAAAGCCTTTTTTCTAAAGGGACTGTATTTCACTTGCGTGGACTGCTTCAAGCATAACCAAACTACTACTTTTGATCTTTGCACCTCCTGCTACCGCATCCGCAACTACGTTCACATACACTGCAACTTTTTGGACAACTACGCCTTGCTAGCTACTTATAGATTGACAAACCAGCCAGCAAGCTCAGGAAAGTCTAGAAGTTTAAAAGCTTTGGGAGCCATCGATAAAGGTGCTCAAATCGTAGATACTGTGGGAAATATTGCGGGAAGTGTTGCTCAATGCACCATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

193

Amino Acids

21.83

Weight (kDa)

5.52

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 12
AciI CCGC 3 cut(s) 382, 388, 551
AclWI GGATC 1 cut(s) 275
AcsI RAATTY 2 cut(s) 18, 49
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 1 cut(s) 80
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 3 cut(s) 92, 139, 337
AjnI CCWGG 1 cut(s) 186
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 4 cut(s) 38, 440, 471, 497
AluI AGCT 4 cut(s) 38, 440, 471, 497
Alw21I GWGCWC 1 cut(s) 523
AlwI GGATC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 2 cut(s) 18, 49
AspS9I GGNCC 1 cut(s) 190
AsuNHI GCTAGC 1 cut(s) 436
AvaII GGWCC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 107, 203, 515
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 1 cut(s) 188
BfaI CTAG 3 cut(s) 194, 437, 483
BfrI CTTAAG 1 cut(s) 254
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 1 cut(s) 188
BmsI GCATC 3 cut(s) 16, 275, 393
BmtI GCTAGC 1 cut(s) 440
Bpu10I CCTNAGC 1 cut(s) 472
Bsa29I ATCGAT 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 1 cut(s) 188
BseCI ATCGAT 1 cut(s) 510
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 290, 384
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 1 cut(s) 486
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 16
BshVI ATCGAT 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 523
BslFI GGGAC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 523
Bsp143I GATC 2 cut(s) 267, 361
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 3 cut(s) 382, 388, 551
BspCNI CTCAG 1 cut(s) 485
BspDI ATCGAT 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 505
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 275
BspTI CTTAAG 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 2 cut(s) 267, 361
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 4 cut(s) 83, 121, 312, 538
BstACI GRCGYC 1 cut(s) 9
BstAFI CTTAAG 1 cut(s) 254
BstAPI GCANNNNNTGC 2 cut(s) 285, 375
BstC8I GCNNGC 3 cut(s) 438, 465, 469
BstDEI CTNAG 1 cut(s) 472
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 290, 384
BstKTI GATC 2 cut(s) 270, 364
BstMBI GATC 2 cut(s) 267, 361
BstMWI GCNNNNNNNGC 6 cut(s) 35, 285, 330, 375, 437, 503
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 16
Bsu15I ATCGAT 1 cut(s) 510
BsuTUI ATCGAT 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 2 cut(s) 290, 384
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 3 cut(s) 438, 465, 469
Cfr13I GGNCC 1 cut(s) 190
ClaI ATCGAT 1 cut(s) 510
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 127, 179
CviJI RGCY 9 cut(s) 38, 177, 260, 293, 440, 463, 471, 497, 506
CviKI_1 RGCY 9 cut(s) 38, 177, 260, 293, 440, 463, 471, 497, 506
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 472
DpnI GATC 2 cut(s) 269, 363
DpnII GATC 2 cut(s) 267, 361
DraI TTTAAA 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 2 cut(s) 130, 182
FaiI YATR 6 cut(s) 128, 180, 342, 404, 447, 578
FaqI GGGAC 1 cut(s) 321
FatI CATG 2 cut(s) 126, 178
FauI CCCGC 1 cut(s) 544
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 297, 371
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 3 cut(s) 194, 437, 483
GluI GCNGC 1 cut(s) 30
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 2 cut(s) 130, 182
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 1 cut(s) 230
Hpy166II GTNNAC 2 cut(s) 328, 400
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 3 cut(s) 23, 474, 483
Hpy8I GTNNAC 2 cut(s) 328, 400
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 4 cut(s) 83, 121, 312, 538
HpyCH4IV ACGT 2 cut(s) 9, 396
HpyCH4V TGCA 5 cut(s) 279, 288, 369, 411, 570
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 285, 330, 375, 437, 503
HpyF3I CTNAG 1 cut(s) 472
HpySE526I ACGT 2 cut(s) 9, 396
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 2 cut(s) 130, 182
Kzo9I GATC 2 cut(s) 267, 361
LmnI GCTCC 1 cut(s) 503
LpnPI CCDG 7 cut(s) 59, 173, 200, 388, 459, 473, 477
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 16, 275, 393
MaeI CTAG 3 cut(s) 194, 437, 483
MaeII ACGT 2 cut(s) 9, 396
MaeIII GTNAC 1 cut(s) 121
MalI GATC 2 cut(s) 269, 363
MboI GATC 2 cut(s) 267, 361
MhlI GDGCHC 1 cut(s) 523
MluCI AATT 3 cut(s) 18, 49, 214
MnlI CCTC 6 cut(s) 67, 124, 145, 220, 258, 382
MseI TTAA 2 cut(s) 255, 491
MslI CAYNNNNRTG 2 cut(s) 323, 575
MspCI CTTAAG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 188
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 6 cut(s) 35, 285, 330, 375, 437, 503
NcoI CCATGG 1 cut(s) 126
NdeII GATC 2 cut(s) 267, 361
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 2 cut(s) 130, 182
NlaIV GGNNCC 1 cut(s) 505
OliI CACNNNNGTG 1 cut(s) 323
PfeI GAWTC 1 cut(s) 230
PflFI GACNNNGTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 505
PspPI GGNCC 1 cut(s) 190
PspPPI RGGWCCY 1 cut(s) 190
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 2 cut(s) 323, 575
SaqAI TTAA 2 cut(s) 255, 491
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 267, 361
Sau96I GGNCC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 523
SetI ASST 9 cut(s) 12, 40, 195, 374, 399, 442, 473, 499, 520
SfaNI GCATC 3 cut(s) 16, 275, 393
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 2 cut(s) 323, 575
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 3 cut(s) 18, 49, 214
SsiI CCGC 3 cut(s) 382, 388, 551
SspI AATATT 1 cut(s) 547
SspMI CTAG 3 cut(s) 194, 437, 483
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 4 cut(s) 83, 121, 312, 538
TaiI ACGT 2 cut(s) 12, 399
TaqI TCGA 1 cut(s) 510
TasI AATT 3 cut(s) 18, 49, 214
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 2 cut(s) 255, 491
Tru9I TTAA 2 cut(s) 255, 491
TscAI CASTG 1 cut(s) 413
TseI GCWGC 1 cut(s) 29
TspRI CASTG 1 cut(s) 413
Tth111I GACNNNGTC 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 18, 49
XbaI TCTAGA 1 cut(s) 482
XspI CTAG 3 cut(s) 194, 437, 483
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.