RchiOBHm_Chr4g0395751

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000286
Physical Location & Seq
Forward (+)
11684819 .. 11686205
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36817

Sequence Viewer

Length: 1023 bp
ATGGAAGAAATACGTGAAGTTGCTTTAGCATACTATGACAATTGCACACCAGAACTTCGGGGTAAGACATGGGAGTTCTTTCAATCCATGGATACGAACGGCGATCACCGAATCAGTGCCAGGGAGTTCAATGAATTCCTCCGTCAGACCGGTTACAACACGATCATTACTGACCCAGAATTATTTGCAAGGCTTGACCGCAACGGTGACTCCGGGCTGGATTTCGATGAAGTACTCACTTTCTACTATATCATTAAAACTGAGTACGTCCGGTGCCGAGGCTGCAACAAGTACCTTTCCGGGCTCTATTTTACTTGCTTCGAATGCTTTGAGGCAGCCAATCCCTCCACTTTTGATCTATGTGATGCTTGTCATCGCAGTAGCAATTTTACTCACCACCACAGTGACCACACACTCTTCTTGGAGAACCACATATTGCTATACTACAAAAGAGGACCAGATGACAGCATGGCAATGGAATATCTATATGCACAAGCTTTAGAGTACTATGATAAAGCAACTCCAGAGCTTAAGAGATCAGTGATGTCTTTCTTTCAATCCATGGTTACACACGGTGCAAACCAAGTCAGCCTCTCTGTGTTCAACGATTTCCTCAAGCAAAATTACTACGACTGGATTTTTAATGATCCACTCTTGTTCCAGAAACTCGACCGCAACCGTGATGGCGGCCTCGATTTCTGGGAATTGCTCACCTTCTACTACATCATCCACACAAGGTACTTCAATTGCCGAGAATGTCAGGTATACCTTGGCGGGCTGTATTTTACCTGCGTTGCTTGCTTTGAGGGAGGAGCTCATTATCGTCGTCATCATCATTCTGATCTCGGCACCTATAATCTATGTGCTGCCTGCTATCGCAGTAGGAATTTCGTCTCTCAACACCCGCACCACACCTTCTTCTTGGATAACCACATATTGCTGCGCTCCAAAAGGGGTCTTCCTCCTTATGCTACACCAAGTCTGGTAAATTACACTGTAATTAAATCTCATCTTGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

340

Amino Acids

40.15

Weight (kDa)

6.04

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 83 9.9e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 797
AccB1I GGYRCC 2 cut(s) 273, 848
AccI GTMKAC 1 cut(s) 765
AciI CCGC 5 cut(s) 199, 673, 687, 774, 905
AclWI GGATC 1 cut(s) 641
AcsI RAATTY 2 cut(s) 134, 886
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 5 cut(s) 234, 266, 293, 506, 740
AflII CTTAAG 1 cut(s) 530
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 5 cut(s) 83, 130, 557, 604, 745
AjnI CCWGG 1 cut(s) 119
AleI CACNNNNGTG 1 cut(s) 402
AluBI AGCT 3 cut(s) 497, 529, 815
AluI AGCT 3 cut(s) 497, 529, 815
Alw21I GWGCWC 1 cut(s) 817
Alw26I GTCTC 1 cut(s) 898
AlwI GGATC 1 cut(s) 641
AoxI GGCC 1 cut(s) 688
ApeKI GCWGC 4 cut(s) 282, 335, 866, 940
ApoI RAATTY 2 cut(s) 134, 886
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 1 cut(s) 945
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 2 cut(s) 214, 301
AsuHPI GGTGA 4 cut(s) 98, 218, 386, 703
AsuII TTCGAA 1 cut(s) 321
AvaII GGWCC 1 cut(s) 455
BanI GGYRCC 2 cut(s) 273, 848
BanII GRGCYC 2 cut(s) 306, 817
BbsI GAAGAC 1 cut(s) 950
Bbv12I GWGCWC 1 cut(s) 817
BbvI GCAGC 4 cut(s) 269, 347, 853, 927
BccI CCATC 1 cut(s) 677
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 1 cut(s) 121
BciVI GTATCC 1 cut(s) 85
BcnI CCSGG 2 cut(s) 214, 301
BcoDI GTCTC 1 cut(s) 898
BfrI CTTAAG 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 797
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 5 cut(s) 283, 336, 688, 867, 941
BlsI GCNGC 5 cut(s) 284, 337, 689, 868, 942
BmcAI AGTACT 2 cut(s) 234, 506
Bme1390I CCNGG 3 cut(s) 121, 214, 301
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 275, 850
BmrFI CCNGG 3 cut(s) 121, 214, 301
BmsI GCATC 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 950
BpmI CTGGAG 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 2 cut(s) 214, 301
BsaAI YACGTR 1 cut(s) 14
BsaJI CCNNGG 5 cut(s) 87, 120, 277, 561, 769
BsaWI WCCGGW 2 cut(s) 149, 270
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 638
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 5 cut(s) 87, 120, 277, 561, 769
BseGI GGATG 1 cut(s) 726
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 638
BseRI GAGGAG 1 cut(s) 825
BseXI GCAGC 4 cut(s) 269, 347, 853, 927
Bsh1285I CGRYCG 1 cut(s) 673
BshFI GGCC 1 cut(s) 690
BshNI GGYRCC 2 cut(s) 273, 848
BshTI ACCGGT 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 673
BsiHKAI GWGCWC 1 cut(s) 817
BsiSI CCGG 4 cut(s) 150, 213, 271, 300
BsmAI GTCTC 1 cut(s) 898
BsmBI CGTCTC 1 cut(s) 898
BsmI GAATGC 1 cut(s) 329
BsnI GGCC 1 cut(s) 690
Bsp119I TTCGAA 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 306, 817
Bsp143I GATC 6 cut(s) 103, 162, 355, 536, 646, 841
Bsp19I CCATGG 2 cut(s) 87, 561
BspACI CCGC 5 cut(s) 199, 673, 687, 774, 905
BspANI GGCC 1 cut(s) 690
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 275, 850
BspMI ACCTGC 1 cut(s) 797
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 321
BspT107I GGYRCC 2 cut(s) 273, 848
BspTI CTTAAG 1 cut(s) 530
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 638
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 5 cut(s) 87, 120, 277, 561, 769
BssMI GATC 6 cut(s) 103, 162, 355, 536, 646, 841
BssNAI GTATAC 1 cut(s) 766
BssT1I CCWWGG 3 cut(s) 87, 561, 769
Bst1107I GTATAC 1 cut(s) 766
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 5 cut(s) 206, 404, 575, 680, 997
Bst6I CTCTTC 1 cut(s) 422
BstAFI CTTAAG 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 14
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 3 cut(s) 776, 799, 871
BstDEI CTNAG 1 cut(s) 261
BstDSI CCRYGG 2 cut(s) 87, 561
BstF5I GGATG 1 cut(s) 726
BstHHI GCGC 1 cut(s) 945
BstKTI GATC 6 cut(s) 106, 165, 358, 539, 649, 844
BstMAI GTCTC 1 cut(s) 898
BstMBI GATC 6 cut(s) 103, 162, 355, 536, 646, 841
BstMCI CGRYCG 1 cut(s) 673
BstMWI GCNNNNNNNGC 3 cut(s) 282, 324, 798
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 3 cut(s) 119, 212, 299
BstV1I GCAGC 4 cut(s) 269, 347, 853, 927
BstV2I GAAGAC 1 cut(s) 950
BstZ17I GTATAC 1 cut(s) 766
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 1 cut(s) 690
BtgI CCRYGG 2 cut(s) 87, 561
BtgZI GCGATG 1 cut(s) 359
BtsCI GGATG 1 cut(s) 726
BtsIMutI CAGTG 4 cut(s) 121, 409, 546, 993
BveI ACCTGC 1 cut(s) 797
Cac8I GCNNGC 3 cut(s) 776, 799, 871
CfoI GCGC 1 cut(s) 945
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 455
Csp6I GTAC 5 cut(s) 233, 265, 292, 505, 739
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 4 cut(s) 69, 88, 469, 562
CviQI GTAC 5 cut(s) 233, 265, 292, 505, 739
DdeI CTNAG 1 cut(s) 261
DpnI GATC 6 cut(s) 105, 164, 357, 538, 648, 843
DpnII GATC 6 cut(s) 103, 162, 355, 536, 646, 841
DraIII CACNNNGTG 1 cut(s) 575
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Ecl136II GAGCTC 1 cut(s) 815
Eco130I CCWWGG 3 cut(s) 87, 561, 769
Eco24I GRGCYC 2 cut(s) 306, 817
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 815
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 3 cut(s) 87, 561, 769
EcoT38I GRGCYC 2 cut(s) 306, 817
ErhI CCWWGG 3 cut(s) 87, 561, 769
Esp3I CGTCTC 1 cut(s) 898
FaeI CATG 4 cut(s) 72, 91, 472, 565
FatI CATG 4 cut(s) 68, 87, 468, 561
FauI CCCGC 2 cut(s) 767, 912
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 5 cut(s) 283, 336, 688, 867, 941
FokI GGATG 1 cut(s) 713
FriOI GRGCYC 2 cut(s) 306, 817
Fsp4HI GCNGC 5 cut(s) 283, 336, 688, 867, 941
GlaI GCGC 1 cut(s) 944
GluI GCNGC 5 cut(s) 283, 336, 688, 867, 941
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 1 cut(s) 690
HapII CCGG 4 cut(s) 150, 213, 271, 300
HhaI GCGC 1 cut(s) 945
Hin1II CATG 4 cut(s) 72, 91, 472, 565
Hin6I GCGC 1 cut(s) 943
HinP1I GCGC 1 cut(s) 943
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 2 cut(s) 111, 209
HpaII CCGG 4 cut(s) 150, 213, 271, 300
HphI GGTGA 4 cut(s) 98, 218, 386, 703
Hpy166II GTNNAC 1 cut(s) 766
Hpy188I TCNGA 2 cut(s) 147, 841
Hpy188III TCNNGA 2 cut(s) 524, 661
Hpy8I GTNNAC 1 cut(s) 766
Hpy99I CGWCG 1 cut(s) 828
HpyAV CCTTC 2 cut(s) 724, 925
HpyCH4III ACNGT 5 cut(s) 206, 404, 575, 680, 997
HpyCH4IV ACGT 2 cut(s) 13, 267
HpyCH4V TGCA 5 cut(s) 45, 188, 285, 491, 578
HpyF10VI GCNNNNNNNGC 3 cut(s) 282, 324, 798
HpyF3I CTNAG 1 cut(s) 261
HpySE526I ACGT 2 cut(s) 13, 267
Hsp92II CATG 4 cut(s) 72, 91, 472, 565
HspAI GCGC 1 cut(s) 943
Kzo9I GATC 6 cut(s) 103, 162, 355, 536, 646, 841
LmnI GCTCC 2 cut(s) 812, 950
Lsp1109I GCAGC 4 cut(s) 269, 347, 853, 927
LweI GCATC 1 cut(s) 355
MaeII ACGT 2 cut(s) 13, 267
MaeIII GTNAC 4 cut(s) 152, 206, 404, 565
MalI GATC 6 cut(s) 105, 164, 357, 538, 648, 843
MboI GATC 6 cut(s) 103, 162, 355, 536, 646, 841
MboII GAAGA 4 cut(s) 17, 409, 910, 950
MfeI CAATTG 2 cut(s) 40, 745
MhlI GDGCHC 2 cut(s) 306, 817
MlyI GAGTC 1 cut(s) 203
MseI TTAA 4 cut(s) 255, 531, 642, 1002
MslI CAYNNNNRTG 2 cut(s) 402, 473
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 4 cut(s) 150, 213, 271, 300
MspR9I CCNGG 3 cut(s) 121, 214, 301
MunI CAATTG 2 cut(s) 40, 745
Mva1269I GAATGC 1 cut(s) 329
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 3 cut(s) 282, 324, 798
NciI CCSGG 2 cut(s) 214, 301
NcoI CCATGG 2 cut(s) 87, 561
NdeII GATC 6 cut(s) 103, 162, 355, 536, 646, 841
NlaIII CATG 4 cut(s) 72, 91, 472, 565
NlaIV GGNNCC 2 cut(s) 275, 850
NmeAIII GCCGAG 3 cut(s) 302, 776, 825
NmuCI GTSAC 2 cut(s) 206, 404
NspV TTCGAA 1 cut(s) 321
OliI CACNNNNGTG 1 cut(s) 402
PctI GAATGC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 5 cut(s) 284, 337, 689, 868, 942
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 817
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 275, 850
PspPI GGNCC 1 cut(s) 455
RsaI GTAC 5 cut(s) 234, 266, 293, 506, 740
RsaNI GTAC 5 cut(s) 233, 265, 292, 505, 739
RseI CAYNNNNRTG 2 cut(s) 402, 473
SacI GAGCTC 1 cut(s) 817
SaqAI TTAA 4 cut(s) 255, 531, 642, 1002
SatI GCNGC 5 cut(s) 283, 336, 688, 867, 941
Sau3AI GATC 6 cut(s) 103, 162, 355, 536, 646, 841
Sau96I GGNCC 1 cut(s) 455
ScaI AGTACT 2 cut(s) 234, 506
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 3 cut(s) 121, 214, 301
SduI GDGCHC 2 cut(s) 306, 817
SfaNI GCATC 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 321
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 2 cut(s) 402, 473
SmlI CTYRAG 2 cut(s) 530, 614
SmoI CTYRAG 2 cut(s) 530, 614
SsiI CCGC 5 cut(s) 199, 673, 687, 774, 905
SstI GAGCTC 1 cut(s) 817
StyD4I CCNGG 3 cut(s) 119, 212, 299
StyI CCWWGG 3 cut(s) 87, 561, 769
TaaI ACNGT 5 cut(s) 206, 404, 575, 680, 997
TaiI ACGT 2 cut(s) 16, 270
TaqI TCGA 4 cut(s) 225, 321, 669, 693
TatI WGTACW 2 cut(s) 232, 504
TauI GCSGC 1 cut(s) 690
TfiI GAWTC 1 cut(s) 111
Tru1I TTAA 4 cut(s) 255, 531, 642, 1002
Tru9I TTAA 4 cut(s) 255, 531, 642, 1002
TscAI CASTG 4 cut(s) 121, 409, 546, 1000
TseFI GTSAC 2 cut(s) 206, 404
TseI GCWGC 4 cut(s) 282, 335, 866, 940
Tsp45I GTSAC 2 cut(s) 206, 404
TspDTI ATGAA 2 cut(s) 147, 243
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 4 cut(s) 121, 409, 546, 1000
Vha464I CTTAAG 1 cut(s) 530
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 2 cut(s) 134, 886
XmiI GTMKAC 1 cut(s) 765
ZrmI AGTACT 2 cut(s) 234, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.