Rorug03G0356100

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000286
Physical Location & Seq
Reverse (-)
44153554 .. 44171005
17452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0356100.1

Sequence Viewer

Length: 300 bp
ATGCTTCTATTTCTCGAATCTTTTCTTTTTTTAATTGGTAAACAAAAAAAAAACTGTTCTGGTTGCAGTCAACAGCGCCACTTCTATCTCGCCGTCGACAGACTCCAGTTCAAGATGGAGACATTTATGGACCTGTTGGGCGTGGCGGGAAGGCGTCCTTGTCTTCCAATGGTGGTCTGCTACAGCTCACGTGACGAGCTCGACGCCGTTAGCTCCACCGTCGCAACCGTCCCCTATATCTCTTTAGCCCCTCTGATGGTCACTCATTGCATGGAGACTTCCAGGGCAAACAGACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

99

Amino Acids

11.25

Weight (kDa)

8.71

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 96
AciI CCGC 1 cut(s) 146
AcvI CACGTG 1 cut(s) 191
AcyI GRCGYC 2 cut(s) 154, 204
AfiI CCNNNNNNNGG 1 cut(s) 256
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 3 cut(s) 186, 199, 213
AluI AGCT 3 cut(s) 186, 199, 213
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 2 cut(s) 113, 269
AlwNI CAGNNNCTG 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 21
AspLEI GCGC 1 cut(s) 78
AspS9I GGNCC 1 cut(s) 130
AvaII GGWCC 1 cut(s) 130
BanII GRGCYC 1 cut(s) 201
BbrPI CACGTG 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 201
BccI CCATC 2 cut(s) 109, 250
BceAI ACGGC 2 cut(s) 77, 191
BciT130I CCWGG 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 113, 269
BfmI CTRYAG 1 cut(s) 181
BfoI RGCGCY 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 283
BpiI GAAGAC 1 cut(s) 155
BpmI CTGGAG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 191
BsaHI GRCGYC 2 cut(s) 154, 204
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 1 cut(s) 106
Bse3DI GCAATG 1 cut(s) 265
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 265
BseNI ACTGG 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 201
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 2 cut(s) 113, 269
BsmFI GGGAC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 201
BspACI CCGC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 1 cut(s) 106
BssECI CCNNGG 1 cut(s) 282
BssNI GRCGYC 2 cut(s) 154, 204
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 3 cut(s) 56, 220, 229
BstACI GRCGYC 2 cut(s) 154, 204
BstBAI YACGTR 1 cut(s) 191
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 1 cut(s) 78
BstMAI GTCTC 2 cut(s) 113, 269
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 181
BstV2I GAAGAC 1 cut(s) 155
BtsIMutI CAGTG 1 cut(s) 295
CaiI CAGNNNCTG 1 cut(s) 297
CfoI GCGC 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 130
CseI GACGC 2 cut(s) 143, 212
CviAII CATG 1 cut(s) 271
CviJI RGCY 4 cut(s) 186, 199, 213, 248
CviKI_1 RGCY 4 cut(s) 186, 199, 213, 248
Ecl136II GAGCTC 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 199
Eco72I CACGTG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 201
FaeI CATG 1 cut(s) 274
FaiI YATR 3 cut(s) 128, 237, 272
FalI AAGNNNNNCTT 2 cut(s) 142, 174
FaqI GGGAC 1 cut(s) 215
FatI CATG 1 cut(s) 270
FauI CCCGC 1 cut(s) 139
FblI GTMKAC 1 cut(s) 96
FriOI GRGCYC 1 cut(s) 201
GlaI GCGC 1 cut(s) 77
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 79
HgaI GACGC 2 cut(s) 143, 212
HhaI GCGC 1 cut(s) 78
Hin1I GRCGYC 2 cut(s) 154, 204
Hin1II CATG 1 cut(s) 274
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HincII GTYRAC 2 cut(s) 71, 97
HindII GTYRAC 2 cut(s) 71, 97
HinfI GANTC 2 cut(s) 17, 102
Hpy166II GTNNAC 3 cut(s) 41, 71, 97
Hpy188I TCNGA 1 cut(s) 255
Hpy188III TCNNGA 2 cut(s) 14, 112
Hpy8I GTNNAC 3 cut(s) 41, 71, 97
Hpy99I CGWCG 3 cut(s) 98, 206, 224
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 3 cut(s) 56, 220, 229
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 2 cut(s) 66, 270
HpySE526I ACGT 1 cut(s) 190
Hsp92I GRCGYC 2 cut(s) 154, 204
Hsp92II CATG 1 cut(s) 274
HspAI GCGC 1 cut(s) 76
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 5 cut(s) 45, 119, 146, 268, 295
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 2 cut(s) 191, 259
MboII GAAGA 1 cut(s) 155
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 1 cut(s) 33
MlyI GAGTC 1 cut(s) 96
MnlI CCTC 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 283
MvaI CCWGG 1 cut(s) 283
NlaIII CATG 1 cut(s) 274
NmuCI GTSAC 2 cut(s) 191, 259
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 1 cut(s) 17
PleI GAGTC 1 cut(s) 96
PmaCI CACGTG 1 cut(s) 191
PmlI CACGTG 1 cut(s) 191
PpsI GAGTC 1 cut(s) 96
Ppu21I YACGTR 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 281
PspCI CACGTG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 281
PspPI GGNCC 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 297
SacI GAGCTC 1 cut(s) 201
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 32
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 1 cut(s) 201
SetI ASST 5 cut(s) 135, 188, 193, 201, 215
SfcI CTRYAG 1 cut(s) 181
SinI GGWCC 1 cut(s) 130
Sse9I AATT 1 cut(s) 33
SsiI CCGC 1 cut(s) 146
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 281
TaaI ACNGT 3 cut(s) 56, 220, 229
TaiI ACGT 1 cut(s) 193
TaqI TCGA 3 cut(s) 15, 96, 201
TasI AATT 1 cut(s) 33
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseFI GTSAC 2 cut(s) 191, 259
Tsp45I GTSAC 2 cut(s) 191, 259
VpaK11BI GGWCC 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.