FvH4_4g04740

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000286
Physical Location & Seq
Forward (+)
4132802 .. 4134407
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04740.t2

Sequence Viewer

Length: 708 bp
ATGAGTAACGTAGATGAAGATGAAGTACGTTTGGCCGCTTTAGCTTATTACAGCAATTTGTCTGAAGAGCTAAAGGAGATGGCAAAAACAACTTTCGACAAAATGGATTCAGATCGCAATGGGAGAATAAGCCTGGAGGAATACCGGAAGCACCTTGCTAATCCCGGTGAGTCTACACAAATGAAATCGAGTTCGTTCAACGTGGTAGACAAAAACGGGGACGGCAAGCTGGATTATTGGGAATTCATCACCCTTTACTATCTCGACAAGAGTGGGAGGCTTAACTGTTGGTGCAATGGCCATGGATGTAAGAATGGCACTTTCCTCTCCGGACTGTATTTTACTTGCGTCACTTGCTTCAGTAGCTCTACAGAACCTGAGTTGTTCTACCTCTGCTGGTCGTGTTATCGCAACAGAAGATATACTCACAAGCACGACAACTTCTTGGACAACTACACCTTCCTCCAAATGAAGATTGCTCCTTGTATACCTGCAGCAACACCAAAAAGTCAGGTTTCTGGTCAGACGTCGACCTCTAATACTGAATCTCAATCATCTAATGAAACTCCGTCGTCGTCCAATAATCCTAAGAAAGCTCGATCTGGCACTCAGACCCTCGCGAATTACAGTAAAGCTCTCAATATCATAAATGTTCTCGCAGAAATTGCTAAAGCTGCTGTTAACGTTGGTGGGTGCAGCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

236

Amino Acids

26.3

Weight (kDa)

6.88

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 26 - 87 3.1e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 530
Acc36I ACCTGC 1 cut(s) 499
AccI GTMKAC 4 cut(s) 173, 207, 487, 530
AccII CGCG 1 cut(s) 620
AccIII TCCGGA 1 cut(s) 329
AciI CCGC 1 cut(s) 36
AclI AACGTT 1 cut(s) 684
AcoI YGGCCR 2 cut(s) 33, 298
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 2 cut(s) 84, 343
AcyI GRCGYC 1 cut(s) 527
AfaI GTAC 1 cut(s) 27
AgsI TTSAA 1 cut(s) 199
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 7 cut(s) 44, 70, 229, 366, 596, 635, 674
AluI AGCT 7 cut(s) 44, 70, 229, 366, 596, 635, 674
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 2 cut(s) 33, 298
ApeKI GCWGC 3 cut(s) 494, 674, 696
ApoI RAATTY 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 179, 241
BalI TGGCCA 1 cut(s) 300
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbvI GCAGC 2 cut(s) 506, 661
BccI CCATC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 238
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 1 cut(s) 165
BfmI CTRYAG 2 cut(s) 369, 492
BfuAI ACCTGC 1 cut(s) 499
BisI GCNGC 4 cut(s) 36, 495, 675, 697
BlsI GCNGC 4 cut(s) 37, 496, 676, 698
Bme1390I CCNGG 2 cut(s) 134, 165
BmrFI CCNGG 2 cut(s) 134, 165
BpmI CTGGAG 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 301
BsaWI WCCGGW 2 cut(s) 144, 329
Bse3DI GCAATG 2 cut(s) 124, 301
Bse8I GATNNNNATC 1 cut(s) 111
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 311
BseJI GATNNNNATC 1 cut(s) 111
BseMI GCAATG 2 cut(s) 124, 301
BseMII CTCAG 2 cut(s) 369, 623
BseXI GCAGC 2 cut(s) 506, 661
Bsh1236I CGCG 1 cut(s) 620
BshFI GGCC 2 cut(s) 35, 300
BsiSI CCGG 3 cut(s) 145, 165, 330
BslFI GGGAC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 2 cut(s) 35, 300
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 2 cut(s) 112, 599
Bsp19I CCATGG 1 cut(s) 301
Bsp68I TCGCGA 1 cut(s) 620
BspACI CCGC 1 cut(s) 36
BspANI GGCC 2 cut(s) 35, 300
BspCNI CTCAG 2 cut(s) 370, 622
BspEI TCCGGA 1 cut(s) 329
BspFNI CGCG 1 cut(s) 620
BspMAI CTGCAG 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 499
BspQI GCTCTTC 1 cut(s) 60
BsrDI GCAATG 2 cut(s) 124, 301
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 2 cut(s) 112, 599
BssNAI GTATAC 1 cut(s) 488
BssNI GRCGYC 1 cut(s) 527
BssT1I CCWWGG 1 cut(s) 301
Bst1107I GTATAC 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 3 cut(s) 287, 336, 629
Bst6I CTCTTC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 3 cut(s) 378, 588, 609
BstDSI CCRYGG 1 cut(s) 301
BstF5I GGATG 1 cut(s) 311
BstFNI CGCG 1 cut(s) 620
BstKTI GATC 2 cut(s) 115, 602
BstMBI GATC 2 cut(s) 112, 599
BstMWI GCNNNNNNNGC 5 cut(s) 41, 354, 363, 665, 674
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 132, 163
BstSFI CTRYAG 2 cut(s) 369, 492
BstUI CGCG 1 cut(s) 620
BstV1I GCAGC 2 cut(s) 506, 661
BstZ17I GTATAC 1 cut(s) 488
BsuRI GGCC 2 cut(s) 35, 300
BtgI CCRYGG 1 cut(s) 301
BtsCI GGATG 1 cut(s) 311
BtuMI TCGCGA 1 cut(s) 620
BveI ACCTGC 1 cut(s) 499
Cac8I GCNNGC 1 cut(s) 227
CaiI CAGNNNCTG 1 cut(s) 377
CseI GACGC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 26
CviAII CATG 1 cut(s) 302
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 3 cut(s) 378, 588, 609
DpnI GATC 2 cut(s) 114, 601
DpnII GATC 2 cut(s) 112, 599
EaeI YGGCCR 2 cut(s) 33, 298
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 301
Eco57I CTGAAG 2 cut(s) 84, 343
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 132
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 1 cut(s) 305
FaiI YATR 5 cut(s) 303, 423, 488, 647, 704
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 301
FblI GTMKAC 4 cut(s) 173, 207, 487, 530
Fnu4HI GCNGC 4 cut(s) 36, 495, 675, 697
FokI GGATG 1 cut(s) 318
Fsp4HI GCNGC 4 cut(s) 36, 495, 675, 697
GluI GCNGC 4 cut(s) 36, 495, 675, 697
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 2 cut(s) 35, 300
HapII CCGG 3 cut(s) 145, 165, 330
HgaI GACGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 1 cut(s) 305
HincII GTYRAC 2 cut(s) 531, 682
HindII GTYRAC 2 cut(s) 531, 682
HinfI GANTC 3 cut(s) 107, 170, 545
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 3 cut(s) 145, 165, 330
HphI GGTGA 2 cut(s) 179, 241
Hpy166II GTNNAC 5 cut(s) 174, 208, 488, 531, 682
Hpy188I TCNGA 4 cut(s) 64, 112, 525, 612
Hpy188III TCNNGA 3 cut(s) 263, 330, 619
Hpy8I GTNNAC 5 cut(s) 174, 208, 488, 531, 682
Hpy99I CGWCG 3 cut(s) 532, 574, 577
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 3 cut(s) 287, 336, 629
HpyCH4IV ACGT 5 cut(s) 9, 28, 201, 527, 684
HpyCH4V TGCA 3 cut(s) 294, 494, 696
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 354, 363, 665, 674
HpyF3I CTNAG 3 cut(s) 378, 588, 609
HpySE526I ACGT 5 cut(s) 9, 28, 201, 527, 684
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 1 cut(s) 305
Kpn2I TCCGGA 1 cut(s) 329
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 2 cut(s) 112, 599
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 2 cut(s) 506, 661
MaeII ACGT 5 cut(s) 9, 28, 201, 527, 684
MaeIII GTNAC 2 cut(s) 5, 349
MalI GATC 2 cut(s) 114, 601
MboI GATC 2 cut(s) 112, 599
MboII GAAGA 4 cut(s) 29, 77, 429, 484
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 4 cut(s) 55, 242, 622, 663
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 7 cut(s) 130, 270, 335, 401, 473, 544, 626
Mox20I TGGCCA 1 cut(s) 300
MroI TCCGGA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 300
MseI TTAA 2 cut(s) 282, 681
Msp20I TGGCCA 1 cut(s) 300
MspI CCGG 3 cut(s) 145, 165, 330
MspR9I CCNGG 2 cut(s) 134, 165
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 620
MwoI GCNNNNNNNGC 5 cut(s) 41, 354, 363, 665, 674
NciI CCSGG 1 cut(s) 165
NcoI CCATGG 1 cut(s) 301
NdeII GATC 2 cut(s) 112, 599
NlaIII CATG 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 349
NruI TCGCGA 1 cut(s) 620
PciSI GCTCTTC 1 cut(s) 60
PfeI GAWTC 2 cut(s) 107, 545
PkrI GCNGC 4 cut(s) 37, 496, 676, 698
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Psp1406I AACGTT 1 cut(s) 684
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PstI CTGCAG 1 cut(s) 496
PstNI CAGNNNCTG 1 cut(s) 377
RruI TCGCGA 1 cut(s) 620
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
SalI GTCGAC 1 cut(s) 529
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 282, 681
SatI GCNGC 4 cut(s) 36, 495, 675, 697
Sau3AI GATC 2 cut(s) 112, 599
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 134, 165
SfcI CTRYAG 2 cut(s) 369, 492
Sse9I AATT 4 cut(s) 55, 242, 622, 663
SsiI CCGC 1 cut(s) 36
StyD4I CCNGG 2 cut(s) 132, 163
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 3 cut(s) 287, 336, 629
TaiI ACGT 5 cut(s) 12, 31, 204, 530, 687
TaqI TCGA 5 cut(s) 96, 188, 264, 530, 598
TasI AATT 4 cut(s) 55, 242, 622, 663
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 2 cut(s) 107, 545
Tru1I TTAA 2 cut(s) 282, 681
Tru9I TTAA 2 cut(s) 282, 681
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 3 cut(s) 494, 674, 696
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 6 cut(s) 30, 36, 197, 235, 485, 576
TspGWI ACGGA 1 cut(s) 558
XapI RAATTY 1 cut(s) 242
XmiI GTMKAC 4 cut(s) 173, 207, 487, 530
ZraI GACGTC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.