FvH4_1g16600

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000307
Physical Location & Seq
Reverse (-)
9562000 .. 9567192
5193 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16600.t1

Sequence Viewer

Length: 2730 bp
ATGGATGAATTCCTCGCCACCGCAGAGAAAAAGGTGCTTGTGGAGATAGTGAAGTTGGCCCAGAAGCGAGGAATGAAGGGTTTTGAAGGTGACTGGAAGCAGTTCCTAATCAGCTATGATAAAAAATTCGGGCTCAGCTTGAGTGATCCCGCAAAAGGTCCACCGATGATGTTGACTGCTTTTCTGAAAAAGCTCAGATTCCAAGCTGATTCAATCAAGTTGCCCATTGCTTCTCCTGCTACTTACTTGTACACGACAAGCTTCTTGATCATAGCTAGGGAGCTTGTAAGATATCATCCTACAGTTGAGGTTCCAGAGGATTCCACCCAGACCCAAGTACTACCTTTATCGTCTCCTTCATCCTCCGGTTTGAAACTTGATCAGGAAATAGTTCCAACAAACTCTACCATCAATGTACTAGTACCTTCATCATCTTCTTCCGCCTTTAATTTGGAAAAGCTTCTTCCAGAGCCTTCATCATCTAGGTCATCTACGTCTTGGACTTATGATGTCTTCCTCAGTTTCAGGGGTGAGGACACCCGCAATAATTTTACAGATCATTTATACAATGCTCTGTGCCGGAGAGGTATTGATACATTTAGGGATACGGAAGAACTTCAGATGGGAAATTCCATTGCACCAGAACTTCGGAAAGCAATTAAAGGATCACAGTTTGCAATCATCATTCTTTCCCCCATGAAAGAATCAGAAGTCATTGAGGAAATAACTGAAAGGATCTCAAACATACTGAACCAGAGGTCATCCCCTCCTGACAGAGGTTTAATAGGAATGGATGCACGTGTAGAAACAGTGGTGTCTTACTTGGATCTAGGGTCAGATAAGGTTCTTACCATAGGTATTTGGGGGATGGGGGGCATTGGTAAGACTACCCTTGCAAAAGAAGTTTACAAGGAGATTCATAACCTATTTCATGTTAGTGGCTTTGTTCGCAATGTTAGATTGCAATCACAAGTGGAGTTGCAGAAACTTGTTTGTGAGTCTTTCTTTGGGGATGGTTACGTAGACACTGTTAAAAAGGGTTCAAAATGGACCAGGTTATTACAGAAAAAGGTGCTTCTTGTTCTAGACGATGTTGATGACTTAAAACAAATTACAAATTTAGTACCAGGAGGATCGGCCTGTGAAGAGAACTTTTGGGGTCCAGGGAGTCGACTCATTATAACAACTAGAGATAGAAGAACATTGACAGAGCTTGGAGTTCTAGAACATAAAATCTATGAGGTTGAAAAACTAAATGATAAGGAAGCTTATCAGCTTCTGTGTCGGAAAGCCTTCAAGAAGGTCAATCCCCCCAAGGAGTTTGTAGAGCTGTCCAAGAGTTTTCTGAAATATGCTTGTGGCCTTCCTTTAGCTCATGAAGTTCTAGGGTCACACTTGTCTGGAAGAAACGCAGATGAATGGCCAGAGGTACTGGATAGACTTGATGAAGATCTGGATAAAGACATTTTTAGTGTGCTTCAAATAAGTTTTGATGGATTACATACTGTAGATAAAAAGATCTTTCTAGACATTGCTTGTTTCTTCAATGGCGAGGATGTTGTTCGTGTGAAGAAGATACTGAAAGGTTGTGGATTTTCTTCCCGAATAGGAATAACTAACCTCGTTGACAAATCTCTGGTTAAAATTGAAAAGGGTAAAGTGTGGATGCATGATTTACTACAGTGCTTGGGTTGGCATATTGTTCGTAGAGAATCTCCTTTCCCTGGAAAACGCAGCAGGTTGTGGCTTGATGACAATGCACACAAATATGAAACCAGAAGGTCATGGCGTGTTGAGGATGCTTGTAATGTTCTCACGGAAAATACGGGAACAACTGCTGTTGAAAGCTTATTCTTAAGCTTGCCTGAAAAAGAAGTAATCCGTTTGAACTCTGACCCGTTCTTAACTATGAGCAAACTGAGGTTGTTGAAAATCTGGAATGTGAACTTTGTGGATGTGCTCTTATCTTATCCCTCTAAAAAATTACGGCTTTTGGAATGGCATGAATGTCCTCTAAAATCTCTATTTTCTTGGTTTACATCTGAACTACTGCTGGTTGAACTCAAGTTACCAAACAGCCGCATTGAAAGATTATGGGATGTATCGGTTTATCAGGTGATCAACACGCTAGTACACATGGATCTTTCCTACTGCCAATATTTAATTATGACCCCACACTTGGGTGCGGTCCCAAATCTTGAGACATTGATTCTTGAAGGTTGTACAGAGTTATTAGAGGTTGTCGGCTATTCATATATGCATCTTCCACATCTGGTTCTATTGAATTTGAAAGGTTGCGTGAGTCTAAGGAAGCTTCCAGAGATAATGAACTTGACATCTCTTCGAACCTTTATTCTTTCAGGATGTACAAACCTCCGAGAGTTTCCTCAGATAGTGGGAAACATGGATTCTTTAGAACTTTATTTAGATGGCACGGCTTTCGGATATCTTCCTGAGCAGCTGAGAGGCCTTATTTTACTATCTCTTAGAGGCTGCAAGAACCTCCAGAAACTTCCTCTCTCCATTTGTAGTATGACATCCCTGAAGTTTCTCTATTTGTCATCATGTTCAAGTATACAAACACTGCCAGAAGATATAGGCCATTTGGAACACTTGGAGGAGCTTGATATGTGCGAAACTGCTATAAGAGAAGTGCCCGAGTCCATTTCATCTCTTAAGAATCTCAAACTTCTGTGTTTCCATGGATGCGCTGAGTTACGCATGCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

910

Amino Acids

103.03

Weight (kDa)

6.48

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 169 - 243 8e-19 TIR domain
TIR_2 PF13676 171 - 242 1.6e-08 TIR domain
NB-ARC PF00931 266 - 434 3.1e-18 NB-ARC domain
NACHT PF05729 285 - 431 7.1e-07 NACHT domain
WHD_ROQ1 PF23282 504 - 572 1.2e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 823 - 905 3.6e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1181
Acc36I ACCTGC 1 cut(s) 1728
AccI GTMKAC 3 cut(s) 1023, 1171, 2575
AciI CCGC 6 cut(s) 21, 150, 441, 541, 2080, 2186
AclWI GGATC 6 cut(s) 140, 673, 743, 834, 1141, 2148
AcoI YGGCCR 1 cut(s) 1421
AcsI RAATTY 5 cut(s) 8, 125, 628, 1117, 2284
AcuI CTGAAG 2 cut(s) 602, 2564
AcvI CACGTG 1 cut(s) 800
AfaI GTAC 9 cut(s) 251, 339, 417, 423, 1125, 1431, 2133, 2224, 2368
AfiI CCNNNNNNNGG 5 cut(s) 155, 776, 1724, 2179, 2180
AflII CTTAAG 2 cut(s) 1855, 2675
AflIII ACRYGT 1 cut(s) 799
AhlI ACTAGT 1 cut(s) 418
AjnI CCWGG 4 cut(s) 1052, 1126, 1162, 1723
AleI CACNNNNGTG 1 cut(s) 2181
Alw21I GWGCWC 1 cut(s) 1962
Alw26I GTCTC 2 cut(s) 357, 2195
AlwI GGATC 6 cut(s) 140, 673, 743, 834, 1141, 2148
AlwNI CAGNNNCTG 1 cut(s) 1279
Ama87I CYCGRG 1 cut(s) 2657
AoxI GGCC 6 cut(s) 57, 1137, 1360, 1421, 2467, 2599
ApeKI GCWGC 3 cut(s) 1734, 2458, 2493
ApoI RAATTY 5 cut(s) 8, 125, 628, 1117, 2284
Asp700I GAANNNNTTC 5 cut(s) 101, 390, 459, 615, 1292
AspLEI GCGC 1 cut(s) 2711
AspS9I GGNCC 5 cut(s) 58, 158, 1050, 1160, 2188
AsuHPI GGTGA 3 cut(s) 101, 542, 2128
AsuII TTCGAA 1 cut(s) 2344
AvaI CYCGRG 1 cut(s) 2657
AvaII GGWCC 4 cut(s) 158, 1050, 1160, 2188
BaeGI GKGCMC 1 cut(s) 2658
BalI TGGCCA 1 cut(s) 1423
BanII GRGCYC 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 800
BbsI GAAGAC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 1962
BbvI GCAGC 3 cut(s) 1746, 2470, 2480
BccI CCATC 6 cut(s) 416, 616, 862, 1007, 1487, 2423
BceAI ACGGC 3 cut(s) 2003, 2451, 2710
BcgI CGANNNNNNTGC 4 cut(s) 1685, 1719, 2422, 2456
BciT130I CCWGG 4 cut(s) 1054, 1128, 1164, 1725
BciVI GTATCC 1 cut(s) 598
BclI TGATCA 3 cut(s) 267, 379, 2118
BcoDI GTCTC 2 cut(s) 357, 2195
BcuI ACTAGT 1 cut(s) 418
BfmI CTRYAG 3 cut(s) 300, 1506, 1679
BfrI CTTAAG 2 cut(s) 1855, 2675
BfuAI ACCTGC 1 cut(s) 1728
BfuI GTATCC 1 cut(s) 598
BglII AGATCT 2 cut(s) 1450, 1518
BisI GCNGC 4 cut(s) 1735, 2080, 2459, 2494
BlpI GCTNAGC 1 cut(s) 134
BlsI GCNGC 4 cut(s) 1736, 2081, 2460, 2495
BmcAI AGTACT 1 cut(s) 339
Bme1390I CCNGG 4 cut(s) 1054, 1128, 1164, 1725
Bme18I GGWCC 4 cut(s) 158, 1050, 1160, 2188
BmeT110I CYCGRG 1 cut(s) 2657
BmgT120I GGNCC 5 cut(s) 58, 158, 1050, 1160, 2188
BmiI GGNNCC 3 cut(s) 312, 1161, 2190
BmrFI CCNGG 4 cut(s) 1054, 1128, 1164, 1725
BmsI GCATC 5 cut(s) 784, 1656, 1789, 2269, 2696
BpiI GAAGAC 1 cut(s) 505
BpmI CTGGAG 1 cut(s) 2489
Bpu10I CCTNAGC 1 cut(s) 2454
Bpu1102I GCTNAGC 1 cut(s) 134
Bpu14I TTCGAA 1 cut(s) 2344
BpuEI CTTGAG 3 cut(s) 160, 2048, 2219
BsaAI YACGTR 2 cut(s) 800, 1021
BsaBI GATNNNNATC 2 cut(s) 964, 1449
BsaJI CCNNGG 4 cut(s) 1163, 1314, 1723, 2701
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 5 cut(s) 155, 776, 1724, 2179, 2180
Bse1I ACTGG 2 cut(s) 98, 1437
Bse3DI GCAATG 4 cut(s) 225, 633, 958, 1530
Bse8I GATNNNNATC 2 cut(s) 964, 1449
BseBI CCWGG 4 cut(s) 1054, 1128, 1164, 1725
BseDI CCNNGG 4 cut(s) 1163, 1314, 1723, 2701
BseJI GATNNNNATC 2 cut(s) 964, 1449
BseLI CCNNNNNNNGG 5 cut(s) 155, 776, 1724, 2179, 2180
BseMI GCAATG 4 cut(s) 225, 633, 958, 1530
BseMII CTCAG 8 cut(s) 148, 208, 532, 1910, 2402, 2445, 2453, 2703
BseNI ACTGG 2 cut(s) 98, 1437
BseRI GAGGAG 1 cut(s) 2633
BseSI GKGCMC 1 cut(s) 2658
BseXI GCAGC 3 cut(s) 1746, 2470, 2480
BshFI GGCC 6 cut(s) 59, 1139, 1362, 1423, 2469, 2601
BsiHKAI GWGCWC 1 cut(s) 1962
BsiHKCI CYCGRG 1 cut(s) 2657
BsiSI CCGG 2 cut(s) 366, 580
BslFI GGGAC 1 cut(s) 2174
BslI CCNNNNNNNGG 5 cut(s) 155, 776, 1724, 2179, 2180
BsmAI GTCTC 2 cut(s) 357, 2195
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 2174
BsnI GGCC 6 cut(s) 59, 1139, 1362, 1423, 2469, 2601
BsoBI CYCGRG 1 cut(s) 2657
Bsp119I TTCGAA 1 cut(s) 2344
Bsp1286I GDGCHC 3 cut(s) 135, 1962, 2658
Bsp1407I TGTACA 3 cut(s) 249, 2222, 2366
Bsp1720I GCTNAGC 1 cut(s) 134
Bsp19I CCATGG 1 cut(s) 2701
BspACI CCGC 6 cut(s) 21, 150, 441, 541, 2080, 2186
BspANI GGCC 6 cut(s) 59, 1139, 1362, 1423, 2469, 2601
BspCNI CTCAG 8 cut(s) 147, 207, 531, 1911, 2401, 2446, 2454, 2704
BspHI TCATGA 1 cut(s) 1375
BspLI GGNNCC 3 cut(s) 312, 1161, 2190
BspMI ACCTGC 1 cut(s) 1728
BspPI GGATC 6 cut(s) 140, 673, 743, 834, 1141, 2148
BspT104I TTCGAA 1 cut(s) 2344
BspTI CTTAAG 2 cut(s) 1855, 2675
BsrDI GCAATG 4 cut(s) 225, 633, 958, 1530
BsrGI TGTACA 3 cut(s) 249, 2222, 2366
BsrI ACTGG 2 cut(s) 98, 1437
BssECI CCNNGG 4 cut(s) 1163, 1314, 1723, 2701
BssNAI GTATAC 1 cut(s) 2576
BssT1I CCWWGG 2 cut(s) 1314, 2701
Bst1107I GTATAC 1 cut(s) 2576
Bst2UI CCWGG 4 cut(s) 1054, 1128, 1164, 1725
Bst4CI ACNGT 6 cut(s) 304, 672, 811, 1030, 1507, 1683
Bst6I CTCTTC 2 cut(s) 1140, 2346
BstAFI CTTAAG 2 cut(s) 1855, 2675
BstAUI TGTACA 3 cut(s) 249, 2222, 2366
BstBAI YACGTR 2 cut(s) 800, 1021
BstBI TTCGAA 1 cut(s) 2344
BstC8I GCNNGC 2 cut(s) 1862, 2723
BstDSI CCRYGG 1 cut(s) 2701
BstENI CCTNNNNNAGG 1 cut(s) 774
BstHHI GCGC 1 cut(s) 2711
BstMAI GTCTC 2 cut(s) 357, 2195
BstMWI GCNNNNNNNGC 2 cut(s) 236, 948
BstNI CCWGG 4 cut(s) 1054, 1128, 1164, 1725
BstNSI RCATGY 1 cut(s) 2725
BstSCI CCNGG 4 cut(s) 1052, 1126, 1162, 1723
BstSFI CTRYAG 3 cut(s) 300, 1506, 1679
BstSLI GKGCMC 1 cut(s) 2658
BstSNI TACGTA 1 cut(s) 1021
BstV1I GCAGC 3 cut(s) 1746, 2470, 2480
BstV2I GAAGAC 1 cut(s) 505
BstX2I RGATCY 5 cut(s) 735, 826, 1450, 1518, 2140
BstYI RGATCY 5 cut(s) 735, 826, 1450, 1518, 2140
BstZ17I GTATAC 1 cut(s) 2576
BsuI GTATCC 1 cut(s) 598
BsuRI GGCC 6 cut(s) 59, 1139, 1362, 1423, 2469, 2601
BtgI CCRYGG 1 cut(s) 2701
BtsI GCAGTG 1 cut(s) 2582
BtsIMutI CAGTG 4 cut(s) 816, 1026, 1688, 2582
BveI ACCTGC 1 cut(s) 1728
Cac8I GCNNGC 2 cut(s) 1862, 2723
CaiI CAGNNNCTG 1 cut(s) 1279
CciI TCATGA 1 cut(s) 1375
CfoI GCGC 1 cut(s) 2711
Cfr13I GGNCC 5 cut(s) 58, 158, 1050, 1160, 2188
CsiI ACCWGGT 1 cut(s) 1052
Csp6I GTAC 9 cut(s) 250, 338, 416, 422, 1124, 1430, 2132, 2223, 2367
CviQI GTAC 9 cut(s) 250, 338, 416, 422, 1124, 1430, 2132, 2223, 2367
EaeI YGGCCR 1 cut(s) 1421
Eam1104I CTCTTC 2 cut(s) 1140, 2346
EarI CTCTTC 2 cut(s) 1140, 2346
EciI GGCGGA 1 cut(s) 430
Eco105I TACGTA 1 cut(s) 1021
Eco130I CCWWGG 2 cut(s) 1314, 2701
Eco147I AGGCCT 1 cut(s) 2469
Eco24I GRGCYC 1 cut(s) 135
Eco32I GATATC 2 cut(s) 293, 2447
Eco47I GGWCC 4 cut(s) 158, 1050, 1160, 2188
Eco57I CTGAAG 2 cut(s) 602, 2564
Eco72I CACGTG 1 cut(s) 800
Eco88I CYCGRG 1 cut(s) 2657
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 4 cut(s) 1052, 1126, 1162, 1723
EcoRV GATATC 2 cut(s) 293, 2447
EcoT14I CCWWGG 2 cut(s) 1314, 2701
EcoT22I ATGCAT 2 cut(s) 1671, 2262
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 2 cut(s) 1314, 2701
Esp3I CGTCTC 1 cut(s) 357
FaqI GGGAC 1 cut(s) 2174
FauI CCCGC 2 cut(s) 157, 548
FbaI TGATCA 3 cut(s) 267, 379, 2118
FblI GTMKAC 3 cut(s) 1023, 1171, 2575
Fnu4HI GCNGC 4 cut(s) 1735, 2080, 2459, 2494
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 1735, 2080, 2459, 2494
GlaI GCGC 1 cut(s) 2710
GluI GCNGC 4 cut(s) 1735, 2080, 2459, 2494
GsuI CTGGAG 1 cut(s) 2489
HaeIII GGCC 6 cut(s) 59, 1139, 1362, 1423, 2469, 2601
HapII CCGG 2 cut(s) 366, 580
HhaI GCGC 1 cut(s) 2711
Hin6I GCGC 1 cut(s) 2709
HinP1I GCGC 1 cut(s) 2709
HincII GTYRAC 3 cut(s) 174, 1172, 1627
HindII GTYRAC 3 cut(s) 174, 1172, 1627
HindIII AAGCTT 6 cut(s) 259, 458, 1266, 1846, 1858, 2312
HpaII CCGG 2 cut(s) 366, 580
HphI GGTGA 3 cut(s) 101, 542, 2128
HpyCH4III ACNGT 6 cut(s) 304, 672, 811, 1030, 1507, 1683
HpyCH4IV ACGT 3 cut(s) 494, 799, 1020
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 948
HpySE526I ACGT 3 cut(s) 494, 799, 1020
HspAI GCGC 1 cut(s) 2709
Ksp22I TGATCA 3 cut(s) 267, 379, 2118
LmnI GCTCC 2 cut(s) 280, 2620
Lsp1109I GCAGC 3 cut(s) 1746, 2470, 2480
LweI GCATC 5 cut(s) 784, 1656, 1789, 2269, 2696
MabI ACCWGGT 1 cut(s) 1052
MaeII ACGT 3 cut(s) 494, 799, 1020
MaeIII GTNAC 5 cut(s) 89, 1016, 1389, 2067, 2715
MflI RGATCY 5 cut(s) 735, 826, 1450, 1518, 2140
MhlI GDGCHC 3 cut(s) 135, 1962, 2658
MlsI TGGCCA 1 cut(s) 1423
MluNI TGGCCA 1 cut(s) 1423
MlyI GAGTC 5 cut(s) 1007, 1167, 1177, 2311, 2669
MmeI TCCRAC 2 cut(s) 419, 1265
Mox20I TGGCCA 1 cut(s) 1423
Mph1103I ATGCAT 2 cut(s) 1671, 2262
MroXI GAANNNNTTC 5 cut(s) 101, 390, 459, 615, 1292
MscI TGGCCA 1 cut(s) 1423
MslI CAYNNNNRTG 3 cut(s) 936, 1767, 2181
Msp20I TGGCCA 1 cut(s) 1423
MspA1I CMGCKG 1 cut(s) 2461
MspCI CTTAAG 2 cut(s) 1855, 2675
MspI CCGG 2 cut(s) 366, 580
MspR9I CCNGG 4 cut(s) 1054, 1128, 1164, 1725
MvaI CCWGG 4 cut(s) 1054, 1128, 1164, 1725
MwoI GCNNNNNNNGC 2 cut(s) 236, 948
NcoI CCATGG 1 cut(s) 2701
NlaIV GGNNCC 3 cut(s) 312, 1161, 2190
NmuCI GTSAC 2 cut(s) 89, 1389
NsiI ATGCAT 2 cut(s) 1671, 2262
NspI RCATGY 1 cut(s) 2725
NspV TTCGAA 1 cut(s) 2344
OliI CACNNNNGTG 1 cut(s) 2181
PaeI GCATGC 1 cut(s) 2725
PagI TCATGA 1 cut(s) 1375
PceI AGGCCT 1 cut(s) 2469
PdmI GAANNNNTTC 5 cut(s) 101, 390, 459, 615, 1292
PfeI GAWTC 9 cut(s) 198, 209, 320, 704, 916, 1712, 2209, 2408, 2680
PkrI GCNGC 4 cut(s) 1736, 2081, 2460, 2495
PleI GAGTC 5 cut(s) 1006, 1167, 1176, 2310, 2668
PmaCI CACGTG 1 cut(s) 800
PmlI CACGTG 1 cut(s) 800
PpsI GAGTC 5 cut(s) 1006, 1167, 1176, 2310, 2668
Ppu21I YACGTR 2 cut(s) 800, 1021
PsiI TTATAA 1 cut(s) 1181
Psp6I CCWGG 4 cut(s) 1052, 1126, 1162, 1723
PspCI CACGTG 1 cut(s) 800
PspGI CCWGG 4 cut(s) 1052, 1126, 1162, 1723
PspN4I GGNNCC 3 cut(s) 312, 1161, 2190
PspPI GGNCC 5 cut(s) 58, 158, 1050, 1160, 2188
PsrI GAACNNNNNNTAC 2 cut(s) 2052, 2084
PstNI CAGNNNCTG 1 cut(s) 1279
PsuI RGATCY 5 cut(s) 735, 826, 1450, 1518, 2140
PvuII CAGCTG 1 cut(s) 2461
RsaI GTAC 9 cut(s) 251, 339, 417, 423, 1125, 1431, 2133, 2224, 2368
RsaNI GTAC 9 cut(s) 250, 338, 416, 422, 1124, 1430, 2132, 2223, 2367
RseI CAYNNNNRTG 3 cut(s) 936, 1767, 2181
SalI GTCGAC 1 cut(s) 1170
SatI GCNGC 4 cut(s) 1735, 2080, 2459, 2494
Sau96I GGNCC 5 cut(s) 58, 158, 1050, 1160, 2188
ScaI AGTACT 1 cut(s) 339
SchI GAGTC 5 cut(s) 1007, 1167, 1177, 2311, 2669
ScrFI CCNGG 4 cut(s) 1054, 1128, 1164, 1725
SduI GDGCHC 3 cut(s) 135, 1962, 2658
SexAI ACCWGGT 1 cut(s) 1052
SfaNI GCATC 5 cut(s) 784, 1656, 1789, 2269, 2696
SfcI CTRYAG 3 cut(s) 300, 1506, 1679
SfuI TTCGAA 1 cut(s) 2344
SinI GGWCC 4 cut(s) 158, 1050, 1160, 2188
SmiMI CAYNNNNRTG 3 cut(s) 936, 1767, 2181
SmlI CTYRAG 5 cut(s) 139, 1855, 2063, 2198, 2675
SmoI CTYRAG 5 cut(s) 139, 1855, 2063, 2198, 2675
SnaBI TACGTA 1 cut(s) 1021
SpeI ACTAGT 1 cut(s) 418
SphI GCATGC 1 cut(s) 2725
SseBI AGGCCT 1 cut(s) 2469
SsiI CCGC 6 cut(s) 21, 150, 441, 541, 2080, 2186
SspI AATATT 1 cut(s) 2159
StuI AGGCCT 1 cut(s) 2469
StyD4I CCNGG 4 cut(s) 1052, 1126, 1162, 1723
StyI CCWWGG 2 cut(s) 1314, 2701
TaaI ACNGT 6 cut(s) 304, 672, 811, 1030, 1507, 1683
TaiI ACGT 3 cut(s) 497, 802, 1023
TaqI TCGA 2 cut(s) 1171, 2344
TatI WGTACW 6 cut(s) 249, 337, 415, 2131, 2222, 2366
TauI GCSGC 1 cut(s) 2082
TfiI GAWTC 9 cut(s) 198, 209, 320, 704, 916, 1712, 2209, 2408, 2680
TscAI CASTG 4 cut(s) 816, 1033, 1688, 2589
TseFI GTSAC 2 cut(s) 89, 1389
TseI GCWGC 3 cut(s) 1734, 2458, 2493
Tsp45I GTSAC 2 cut(s) 89, 1389
TspGWI ACGGA 3 cut(s) 623, 1832, 1871
TspRI CASTG 4 cut(s) 816, 1033, 1688, 2589
Vha464I CTTAAG 2 cut(s) 1855, 2675
VpaK11BI GGWCC 4 cut(s) 158, 1050, 1160, 2188
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 5 cut(s) 8, 125, 628, 1117, 2284
XbaI TCTAGA 3 cut(s) 1084, 1222, 1525
XceI RCATGY 1 cut(s) 2725
XmiI GTMKAC 3 cut(s) 1023, 1171, 2575
XmnI GAANNNNTTC 5 cut(s) 101, 390, 459, 615, 1292
ZrmI AGTACT 1 cut(s) 339
Zsp2I ATGCAT 2 cut(s) 1671, 2262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.