Rh2CG192500

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000307
Physical Location & Seq
Reverse (-)
18157435 .. 18164730
7296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG192500.1

Sequence Viewer

Length: 5322 bp
ATGCATGATTTGCTACGGTGCTTGGGTTGGCATATTGTTCGTGGAGAATCTTCTTTTCCGGGTAAACGTAGCAGGTTGTGGCTTGATGACAATGCACACAAGTACGAAGGCAGAATGTCATGGCGTTTTGAGGATGCTTGGAACGTGCTGATGGACAATACGGGAACAACTGCTGTTGAAGGCTTATTCTTAAGCTTGCCTGAAAAAGAAGAAATGCCATTGGATGATGACCCGTTCTTAACTATGCGCAACTTACGATTGCTGAAGATTTATAATGTGAACTTTCTGGATGTGCATTTCAGATATGTCTCTAAAAATTTACGACTTTTAGAATGGCACGAATGCCCTCTAGAATCTTTGCCATCTGATTTTAAATCGGACAAGCTGGTTGAATTCAAGATGCCTAACAGCCGCATTAAGCAACTATGGAATGAAACGCTCTCTCTTAGATTGCTAATACTCATGGATCTTTCCAACTGCCAATATTTAGCCACGACCCCAGACTTCAGTAAGGTTCCAAATCTTGAGAGATTGATCCTTGAAGGTTGTACTGAGTTATCAGTAGTTCACCCAACAATTGGGGATCTCCAGCAACTGGTTTTTCTGAATCTGAAAGGTTGCGCAAGTCTAGAGAGCCTTCCTCAGTCCATCAGCTTGAGATCTCTTCGAATTTTTATTCTTTCGGGATGTTCAAAACTCGAACACTTTCCCGAGATTGTGGGGAACATGGATGCTTTATCAGAACTTTATTTAGATGGCACGGCTATACGGGTGCTGCCTTTATCAATCCAGCATTTGGAAGGCCTTGTTTTGCTAAATCTTAGCGGCTGCAGGAACCTTCCGAGTCTTCCTAGTGTTCTTTGTAGTCATTTGACATCACTGAAATTTCTCTATCTGTCATTGTGCTCAAGCATGGAAAAACTGCCAGAAAACATAGGCTACTTGAAACACTTGGAGGAGCTTGATGCTTGTGATACTGCTATAAGAAAAGTTCCTGATTCCATTTCAGGTCTCAAGAATCTAAAACTATTGTGTTTCCATGGATGTTCTGGATTCACAGGTTTAGAGTTGCCAAACAAGTTCTCAGGTTTAAGATCTTTGACAACGCTAAACCTAGGTGGATGTAATCTCGCAGAAGGGGCAATCCCCCATGACATTGGTGATTTATTCTCACTGCAAAGTCTGGATTTAAGTGAGAACAACATTTTCACTGTACCTGAAAGCATCTCTCAGCTTTCGGAGCTCGCAGAAATTTCTCTGTATAAGTGTAGCAAACTACGGTCGTTGCCAAAAGATCTTCCATCAAGTCTAAGGAGTGTAAATGTACGTGATTGTCCTATGCTGATAAACTCTTTATCTAGTTGGAGGAGATATCCTCCGCAGAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGAGGATGACATCTTTCCTGAACTCCATGAGTTTCGTTTGGGAACTCTGGAGGAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTGAAGAAATTGATCCTTGAAGGTTGTGAAAAACTATCAGAACTTCACCCAACAATTGGGAGTCTCCAACATCTGATTTTTTTGAATTTGAAAGGATGTGTAGATCTAGAGAGCCTTCCCCGTTTCATCAGCTTGAAGTCTCTAAAAATATTTGTTCTTTCAGGATGTTCAAAGCTGAAAGAGTTTCCAGATATTAAGGGAGATATGGATAGTTTGTCTCAACTGCATTTAGATGGGACGGCATTAAGGCGTCTATCAATACCGACGAAGCATTTGAAATGCCCTATTTCGATAAATCTAAGAGGCTGTAGGAACCTTTTCACTGTTCCAGTTCTACGAAGTCTGAAATCTCTTAATCTGTCGGGCTGCTCACGTGTATCCATATTGCCATCTAACCTGGAAAGCATGAAACATTTGGAGGAGCTTGATGCCTCTGAAACTTCTATAACAGCGGTACCCTCGTCCATTTCATATTTGGTGAAGCTTGAAGTGCTATCTTTTTGTGGATGTAGTGGTTTGCAGTTGCCTAAATGGTTCTCCGATTTACGATCTTTGAAATCATTAAATCTACGGAGGTGTGGTCTAGCAGAAGAAGCGCTTGATATCCTGTACTGCTTATCCTCTCTGAAGATTTTGGATTTGGGTGAGAACAGTTTTGTGACTATACCTAATGACATCGATCGCTTATCCTCTTTGCAGCAATTGGATTTGAGTGGAAACAATATAGTGAGCATACCGCATGAAATTGGTTGCTTATACTCGTTGCAGCTTTTAGATTTGAGTGAAAACAACTTTGTGAGTATACCTAAGAGCATCTCTAATCTTTCTGAGCTTACAATACTTCGCCTGTTTAGATGTAGTAAGCTACAGTCCTTGCCAAATAATCTTCCCTTCAATCTAAAACATGTTTATGCACAAGAATGTCCTATGTTGAAGTATAATGCAGATACTTTGACAATACGGGCATCCGGAGAAGGGGTCTTTTTGATGCATTGCGGAAAATATGAGCAGGATGATGATCAGCCAAGCCACGTCCCAGTTCCAGCTCCCAAAGACCGCATTGAACTGCTGTTTCCTGTTTACATTGCGGACCGAGTGTATGGTAAAAAGCCATTTGCAATTTGTTTTCCACACAGTACAAGAATTCCCAATCTGTGGAGTCATTGGAGGAGTGGCCCTTTCGTAACAATCCCACTATCTGATGGTAACAGTGCGTGTATGGGACTTGCTCTGTTTGTTGTTTTTGAAATCCTTGAAGGCAATTTCAATAAGATATGGGAATTGGAAGAGACTATCTGTGAATTTCACACCGATGTTGGTCCTGAAAATTCCCTTGTTTTTCAAAACTTCATAGACTTCAAGGCTGGGTCATATGCACTTTGTTGCTATGAACCGCGAGGGGGGCAATTTAGTGGGATGTTCGATAAACCAAGCTCACAACTTCGAGCTTCAGTTTCAATGAAGAGACCAAATTTAAAAGTGAGAGGTTGTGGGATACATCTAATGTCAGAGGAAGACACTGCAGAGTTTGTAAAAAGTTTAGCAAATCAGACTGCCACTCAACACCTCGATTCCAATTTCAATCAACATTGCGAGTACCTATTAAATGAGGAGACAACAACTGGTGACCTGATGGATGTGGGATCTACTTCAACTTTCAATGAAGATAGCTGCAGCAAAAGTTTCTCTAAATTCAAATTGAGAGGAGAGCTGTCGATACTCTATGAGGGATGCAATGGACGTGAGAATAGTTTCCATTTTTGCTTTCCTGCTCCCGTAATTTCAGCCCCACCCTGGTTCTTCCATCACCATGCAGGGGATGTAACTTTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCTACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATATTTGCTCTCATGATCATGAAAGTATAGTAAGGCACGGATCCCTCAAGATAAACAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAGCAGTTGAATCAATGTCAGGGTATTAGCGCATTGTTCAGAACCATAGATGCAGAGATGGATGTCCAAGTGTGTGGAAGCCGTTTAGCTTTCGAGCATGATTTGGAAGACTTGATCCATTCATTAACTGCTGCTGTCGGCACGTTGGGGCAACATGTCCTTACTCAACCACGCTCCCCGGCCCAACCTGTTGATCGGCGCAATGCAGAGGAAGCAGAAATGCCCATCAATTGCTGTTCCTGTTTTCAGAGATGCAGAAGTACTGCACTAGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGCAGCTGCGAAATTATAGATGCTATCTCCAGCAACAGCAAGGTTTTGGGGAGTCTGACGTTAACCAGTTGGTCCACTTCAGATCAGTAATGTTACTCCACTCCAGAAGCCTTTTGGAAAACAGGGACCGAAACCAAGAAAACATTGCCGAAAAGGATAAATCGTTGGTGTTGGCTCATCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGCTATAGTTCACCACAGTGGAAGAGATGCCTGAAGTTGTTGCTTAGGCGGTACAAGGTGGCTACTCTCAGTCTCGGTGGACATGAAATTTCAGCTCTCAAGAATTTTGATCCTTCCTCCCCATACAATATTATTTGTTTCTCTGACAACGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAATTGGACAGGACTTGCTATATGTGTTACCTTTGAAGTTCATGACCAGCGTCCAGCTACCTCCCCAGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTTCCATCACTAAAGAGAAACTCAAGTCGTTGCATCTTGGTCGATTCATTTGGGTAACCTACATCCCCAGTATTTTTCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTGGTTGCAGAGGCTTGACAGTGGACAAGTGTGGTATGCGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAATGCTGGACCTCTTTCTTCGATAATCTGTCTTTCATCCGTCAACTTGTAGAAGCAGATGATCAACCGCATGAACTTCCAATGCTTGAAGTGCGTACCAAGGTCTTTGATCCGGATTTAATATACAATGCAATCTGCCCTTCTAATGAAATTCTGGAGTGGTTCGGGCATCGAATTGAGGATTCCTCGGATGCCAGCTGGGGCTGCGAATTCCAGTTACCACCATCTCTGAGCGACACAAATTGGATAGGATTGTCCATCTGTGTATCATACCGTATTAGAAGAGTGGATTGGCACGAACCTAATTCCCACCCCTTTAATTTAACATTGAAAACCAGAAACAATGCTTTGTCGTCTCTGCATCGGTATCAAAGTAGCAATGAAGAATATCAGTTCGTAAACAGTTGTCGTATTATGAATCGTAGATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGTCCTCTTTGCTTCACTCCGGGACACCTGGTGGAGCCCGGCCAAGATCTATCACCGATTTGTATATGCGCAGGAAGAGTTCAAGCAGCTCTGTTTCAATCTCGATCGACCTGGTGCCCGTGCCCCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

1773

Amino Acids

201.52

Weight (kDa)

6.15

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 191 - 296 3.4e-06 Leucine-rich repeat region
LRR_13 PF23286 219 - 287 4.4e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 262 - 372 1e-12 Leucine-rich repeat region
LRR_14 PF23598 366 - 430 9.6e-06 Leucine-rich repeat region
LRR_14 PF23598 475 - 589 4.6e-06 Leucine-rich repeat region
LRR_14 PF23598 615 - 725 2.1e-10 Leucine-rich repeat region
LRR_14 PF23598 714 - 801 1.6e-10 Leucine-rich repeat region
LRR_8 PF13855 717 - 753 3e-07 Leucine rich repeat
C-JID PF20160 1422 - 1559 5.7e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
Acc16I TGCGCA 3 cut(s) 248, 622, 5259
Acc36I ACCTGC 1 cut(s) 63
Acc65I GGTACC 1 cut(s) 1987
AccB1I GGYRCC 2 cut(s) 1987, 5303
AccB7I CCANNNNNTGG 5 cut(s) 578, 595, 796, 1589, 2688
AccI GTMKAC 1 cut(s) 2335
AccII CGCG 1 cut(s) 2929
AccIII TCCGGA 2 cut(s) 2501, 4816
AcoI YGGCCR 1 cut(s) 5229
AcuI CTGAAG 8 cut(s) 284, 490, 1556, 2180, 2967, 3967, 4100, 4175
AcvI CACGTG 2 cut(s) 1907, 3587
AcyI GRCGYC 1 cut(s) 1783
AdeI CACNNNGTG 1 cut(s) 5220
AfeI AGCGCT 1 cut(s) 2130
AflII CTTAAG 1 cut(s) 190
AflIII ACRYGT 5 cut(s) 1906, 2437, 3577, 3586, 3754
AhlI ACTAGT 1 cut(s) 3868
AjiI CACGTC 2 cut(s) 2566, 3275
AjnI CCWGG 4 cut(s) 1929, 3326, 5216, 5299
AjuI GAANNNNNNNTTGG 2 cut(s) 4978, 5010
AleI CACNNNNGTG 1 cut(s) 4138
Alw21I GWGCWC 2 cut(s) 908, 1245
AlwNI CAGNNNCTG 1 cut(s) 595
Ama87I CYCGRG 1 cut(s) 710
Aor13HI TCCGGA 2 cut(s) 2501, 4816
Aor51HI AGCGCT 1 cut(s) 2130
AoxI GGCC 5 cut(s) 802, 2707, 3780, 4605, 5229
ArsI GACNNNNNNTTYG 2 cut(s) 1789, 1821
Asp700I GAANNNNTTC 3 cut(s) 705, 1850, 3284
Asp718I GGTACC 1 cut(s) 1987
AspA2I CCTAGG 1 cut(s) 1114
AspLEI GCGC 6 cut(s) 249, 623, 2131, 3632, 3801, 5260
AspS9I GGNCC 8 cut(s) 2623, 2708, 2852, 3552, 3781, 3976, 4030, 4713
AsuC2I CCSGG 4 cut(s) 60, 3779, 5210, 5228
AsuHPI GGTGA 9 cut(s) 560, 1172, 1571, 2023, 2189, 3170, 3332, 4125, 5234
AsuII TTCGAA 1 cut(s) 667
AvaI CYCGRG 1 cut(s) 710
AvaII GGWCC 6 cut(s) 2623, 2852, 3552, 3976, 4030, 4713
AvrII CCTAGG 1 cut(s) 1114
BaeGI GKGCMC 2 cut(s) 5308, 5314
BaeI ACNNNNGTAYC 4 cut(s) 1971, 1979, 2004, 2012
BamHI GGATCC 1 cut(s) 3524
BanI GGYRCC 2 cut(s) 1987, 5303
BanII GRGCYC 2 cut(s) 1245, 5228
BarI GAAGNNNNNNTAC 2 cut(s) 3430, 3462
BauI CACGAG 1 cut(s) 4358
BbrPI CACGTG 2 cut(s) 1907, 3587
BbsI GAAGAC 4 cut(s) 839, 3054, 3714, 4308
Bbv12I GWGCWC 2 cut(s) 908, 1245
BceAI ACGGC 3 cut(s) 777, 1788, 3666
BcgI CGANNNNNNTGC 4 cut(s) 20, 54, 4517, 4551
BciT130I CCWGG 4 cut(s) 1931, 3328, 5218, 5301
BciVI GTATCC 2 cut(s) 1921, 3021
BclI TGATCA 4 cut(s) 2551, 3391, 3499, 4765
BcnI CCSGG 4 cut(s) 60, 3779, 5210, 5228
BcuI ACTAGT 1 cut(s) 3868
BfmI CTRYAG 6 cut(s) 829, 1840, 2399, 3054, 3205, 4126
BfoI RGCGCY 1 cut(s) 2132
BfrI CTTAAG 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 63
BfuI GTATCC 2 cut(s) 1921, 3021
BglII AGATCT 5 cut(s) 659, 1094, 1294, 1636, 5235
BlnI CCTAGG 1 cut(s) 1114
BmcAI AGTACT 2 cut(s) 3862, 3873
Bme1390I CCNGG 8 cut(s) 60, 1931, 3328, 3779, 5210, 5218, 5228, 5301
Bme18I GGWCC 6 cut(s) 2623, 2852, 3552, 3976, 4030, 4713
BmeT110I CYCGRG 1 cut(s) 710
BmgBI CACGTC 2 cut(s) 2566, 3275
BmgT120I GGNCC 8 cut(s) 2623, 2708, 2852, 3552, 3781, 3976, 4030, 4713
BmrFI CCNGG 8 cut(s) 60, 1931, 3328, 3779, 5210, 5218, 5228, 5301
BmrI ACTGGG 3 cut(s) 2564, 4427, 4557
BmuI ACTGGG 3 cut(s) 2564, 4427, 4557
BoxI GACNNNNGTC 1 cut(s) 4416
BpiI GAAGAC 4 cut(s) 839, 3054, 3714, 4308
BplI GAGNNNNNCTC 6 cut(s) 625, 657, 1360, 1392, 4874, 4906
BpmI CTGGAG 5 cut(s) 572, 1487, 3917, 3991, 4880
Bpu10I CCTNAGC 1 cut(s) 4166
Bpu14I TTCGAA 1 cut(s) 667
BpuEI CTTGAG 8 cut(s) 545, 676, 892, 998, 3515, 4205, 4474, 4502
BpuMI CCSGG 4 cut(s) 60, 3779, 5210, 5228
Bsa29I ATCGAT 1 cut(s) 2211
BsaAI YACGTR 3 cut(s) 1328, 1907, 3587
BsaBI GATNNNNATC 1 cut(s) 2550
BsaHI GRCGYC 1 cut(s) 1783
BsaI GGTCTC 2 cut(s) 1016, 2992
BsaJI CCNNGG 7 cut(s) 1039, 1114, 3326, 3777, 4461, 4803, 4890
BsaWI WCCGGW 2 cut(s) 2501, 4816
BsaXI ACNNNNNCTCC 4 cut(s) 2098, 2128, 2496, 2526
Bse118I RCCGGY 1 cut(s) 4668
Bse1I ACTGG 9 cut(s) 600, 1862, 2570, 3160, 3970, 4280, 4433, 4563, 4918
Bse3DI GCAATG 7 cut(s) 2524, 2616, 3121, 3274, 3808, 4047, 5089
Bse8I GATNNNNATC 1 cut(s) 2550
BseAI TCCGGA 2 cut(s) 2501, 4816
BseBI CCWGG 4 cut(s) 1931, 3328, 5218, 5301
BseCI ATCGAT 1 cut(s) 2211
BseDI CCNNGG 7 cut(s) 1039, 1114, 3326, 3777, 4461, 4803, 4890
BseJI GATNNNNATC 1 cut(s) 2550
BseMI GCAATG 7 cut(s) 2524, 2616, 3121, 3274, 3808, 4047, 5089
BseMII CTCAG 9 cut(s) 543, 656, 1098, 1244, 2352, 3570, 4095, 4204, 4925
BseNI ACTGG 9 cut(s) 600, 1862, 2570, 3160, 3970, 4280, 4433, 4563, 4918
BseRI GAGGAG 7 cut(s) 971, 1381, 1433, 1967, 2716, 3158, 3252
BseSI GKGCMC 2 cut(s) 5308, 5314
BseYI CCCAGC 2 cut(s) 2897, 4902
BsgI GTGCAG 2 cut(s) 3849, 3926
Bsh1236I CGCG 1 cut(s) 2929
Bsh1285I CGRYCG 3 cut(s) 1283, 2215, 5296
BshFI GGCC 5 cut(s) 804, 2709, 3782, 4607, 5231
BshNI GGYRCC 2 cut(s) 1987, 5303
BshVI ATCGAT 1 cut(s) 2211
BsiEI CGRYCG 3 cut(s) 1283, 2215, 5296
BsiHKAI GWGCWC 2 cut(s) 908, 1245
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 7 cut(s) 59, 2502, 3779, 4669, 4817, 5209, 5228
BslFI GGGAC 6 cut(s) 1783, 2552, 2769, 3565, 4043, 5225
BsmBI CGTCTC 2 cut(s) 4664, 5064
BsmFI GGGAC 6 cut(s) 1783, 2552, 2769, 3565, 4043, 5225
BsmI GAATGC 2 cut(s) 347, 4712
BsnI GGCC 5 cut(s) 804, 2709, 3782, 4607, 5231
Bso31I GGTCTC 2 cut(s) 1016, 2992
BsoBI CYCGRG 1 cut(s) 710
Bsp119I TTCGAA 1 cut(s) 667
Bsp1286I GDGCHC 5 cut(s) 908, 1245, 5228, 5308, 5314
Bsp13I TCCGGA 2 cut(s) 2501, 4816
Bsp1407I TGTACA 2 cut(s) 3437, 4090
Bsp19I CCATGG 1 cut(s) 1039
BspANI GGCC 5 cut(s) 804, 2709, 3782, 4607, 5231
BspCNI CTCAG 9 cut(s) 544, 655, 1097, 1243, 2353, 3569, 4096, 4203, 4926
BspDI ATCGAT 1 cut(s) 2211
BspEI TCCGGA 2 cut(s) 2501, 4816
BspFNI CGCG 1 cut(s) 2929
BspHI TCATGA 3 cut(s) 3496, 3502, 4408
BspMAI CTGCAG 3 cut(s) 833, 3058, 3209
BspMI ACCTGC 1 cut(s) 63
BspT104I TTCGAA 1 cut(s) 667
BspT107I GGYRCC 2 cut(s) 1987, 5303
BspTI CTTAAG 1 cut(s) 190
BspTNI GGTCTC 2 cut(s) 1016, 2992
BsrDI GCAATG 7 cut(s) 2524, 2616, 3121, 3274, 3808, 4047, 5089
BsrFI RCCGGY 1 cut(s) 4668
BsrGI TGTACA 2 cut(s) 3437, 4090
BsrI ACTGG 9 cut(s) 600, 1862, 2570, 3160, 3970, 4280, 4433, 4563, 4918
BssAI RCCGGY 1 cut(s) 4668
BssECI CCNNGG 7 cut(s) 1039, 1114, 3326, 3777, 4461, 4803, 4890
BssNAI GTATAC 1 cut(s) 2336
BssNI GRCGYC 1 cut(s) 1783
BssSI CACGAG 1 cut(s) 4358
BssT1I CCWWGG 4 cut(s) 1039, 1114, 4461, 4803
Bst1107I GTATAC 1 cut(s) 2336
Bst2BI CACGAG 1 cut(s) 4358
Bst2UI CCWGG 4 cut(s) 1931, 3328, 5218, 5301
Bst6I CTCTTC 7 cut(s) 669, 2814, 2990, 4139, 4671, 4981, 5259
BstACI GRCGYC 1 cut(s) 1783
BstAFI CTTAAG 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 10
BstAUI TGTACA 2 cut(s) 3437, 4090
BstBAI YACGTR 3 cut(s) 1328, 1907, 3587
BstBI TTCGAA 1 cut(s) 667
BstC8I GCNNGC 3 cut(s) 197, 1245, 4900
BstDSI CCRYGG 1 cut(s) 1039
BstEII GGTNACC 2 cut(s) 3158, 4549
BstFNI CGCG 1 cut(s) 2929
BstH2I RGCGCY 1 cut(s) 2132
BstHHI GCGC 6 cut(s) 249, 623, 2131, 3632, 3801, 5260
BstMCI CGRYCG 3 cut(s) 1283, 2215, 5296
BstMWI GCNNNNNNNGC 9 cut(s) 10, 1139, 1240, 2023, 2126, 2935, 3812, 4795, 4908
BstNI CCWGG 4 cut(s) 1931, 3328, 5218, 5301
BstNSI RCATGY 3 cut(s) 2441, 3581, 3758
BstPAI GACNNNNGTC 1 cut(s) 4416
BstPI GGTNACC 2 cut(s) 3158, 4549
BstSCI CCNGG 8 cut(s) 58, 1929, 3326, 3777, 5208, 5216, 5226, 5299
BstSFI CTRYAG 6 cut(s) 829, 1840, 2399, 3054, 3205, 4126
BstSLI GKGCMC 2 cut(s) 5308, 5314
BstUI CGCG 1 cut(s) 2929
BstV2I GAAGAC 4 cut(s) 839, 3054, 3714, 4308
BstXI CCANNNNNNTGG 3 cut(s) 1157, 3674, 5160
BstZ17I GTATAC 1 cut(s) 2336
Bsu15I ATCGAT 1 cut(s) 2211
BsuI GTATCC 2 cut(s) 1921, 3021
BsuRI GGCC 5 cut(s) 804, 2709, 3782, 4607, 5231
BsuTUI ATCGAT 1 cut(s) 2211
BtgI CCRYGG 1 cut(s) 1039
BtrI CACGTC 2 cut(s) 2566, 3275
BtsI GCAGTG 2 cut(s) 1172, 3051
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 3 cut(s) 197, 1245, 4900
CaiI CAGNNNCTG 1 cut(s) 595
CciI TCATGA 3 cut(s) 3496, 3502, 4408
CfoI GCGC 6 cut(s) 249, 623, 2131, 3632, 3801, 5260
Cfr10I RCCGGY 1 cut(s) 4668
Cfr13I GGNCC 8 cut(s) 2623, 2708, 2852, 3552, 3781, 3976, 4030, 4713
ClaI ATCGAT 1 cut(s) 2211
CpoI CGGWCCG 1 cut(s) 2623
CseI GACGC 4 cut(s) 1772, 4406, 4646, 5166
CsiI ACCWGGT 2 cut(s) 5216, 5299
CspI CGGWCCG 1 cut(s) 2623
DraI TTTAAA 2 cut(s) 373, 3009
DraIII CACNNNGTG 1 cut(s) 5220
EaeI YGGCCR 1 cut(s) 5229
Eam1104I CTCTTC 7 cut(s) 669, 2814, 2990, 4139, 4671, 4981, 5259
EarI CTCTTC 7 cut(s) 669, 2814, 2990, 4139, 4671, 4981, 5259
Ecl136II GAGCTC 1 cut(s) 1243
Eco130I CCWWGG 4 cut(s) 1039, 1114, 4461, 4803
Eco147I AGGCCT 1 cut(s) 804
Eco24I GRGCYC 2 cut(s) 1245, 5228
Eco31I GGTCTC 2 cut(s) 1016, 2992
Eco32I GATATC 2 cut(s) 1373, 2137
Eco47I GGWCC 6 cut(s) 2623, 2852, 3552, 3976, 4030, 4713
Eco47III AGCGCT 1 cut(s) 2130
Eco53kI GAGCTC 1 cut(s) 1243
Eco57I CTGAAG 8 cut(s) 284, 490, 1556, 2180, 2967, 3967, 4100, 4175
Eco72I CACGTG 2 cut(s) 1907, 3587
Eco88I CYCGRG 1 cut(s) 710
Eco91I GGTNACC 2 cut(s) 3158, 4549
EcoICRI GAGCTC 1 cut(s) 1243
EcoO109I RGGNCCY 1 cut(s) 3552
EcoO65I GGTNACC 2 cut(s) 3158, 4549
EcoRI GAATTC 3 cut(s) 392, 2676, 4913
EcoRII CCWGG 4 cut(s) 1929, 3326, 5216, 5299
EcoRV GATATC 2 cut(s) 1373, 2137
EcoT14I CCWWGG 4 cut(s) 1039, 1114, 4461, 4803
EcoT22I ATGCAT 2 cut(s) 6, 2526
EcoT38I GRGCYC 2 cut(s) 1245, 5228
ErhI CCWWGG 4 cut(s) 1039, 1114, 4461, 4803
Esp3I CGTCTC 2 cut(s) 4664, 5064
FalI AAGNNNNNCTT 2 cut(s) 2115, 2147
FaqI GGGAC 6 cut(s) 1783, 2552, 2769, 3565, 4043, 5225
FauNDI CATATG 1 cut(s) 2905
FbaI TGATCA 4 cut(s) 2551, 3391, 3499, 4765
FblI GTMKAC 1 cut(s) 2335
FriOI GRGCYC 2 cut(s) 1245, 5228
FspI TGCGCA 3 cut(s) 248, 622, 5259
GlaI GCGC 6 cut(s) 248, 622, 2130, 3631, 3800, 5259
GsaI CCCAGC 2 cut(s) 2901, 4906
GsuI CTGGAG 5 cut(s) 572, 1487, 3917, 3991, 4880
HaeII RGCGCY 1 cut(s) 2132
HaeIII GGCC 5 cut(s) 804, 2709, 3782, 4607, 5231
HapII CCGG 7 cut(s) 59, 2502, 3779, 4669, 4817, 5209, 5228
HgaI GACGC 4 cut(s) 1772, 4406, 4646, 5166
HhaI GCGC 6 cut(s) 249, 623, 2131, 3632, 3801, 5260
Hin1I GRCGYC 1 cut(s) 1783
Hin6I GCGC 6 cut(s) 247, 621, 2129, 3630, 3799, 5258
HinP1I GCGC 6 cut(s) 247, 621, 2129, 3630, 3799, 5258
HincII GTYRAC 2 cut(s) 3967, 4748
HindII GTYRAC 2 cut(s) 3967, 4748
HindIII AAGCTT 2 cut(s) 193, 2015
HpaI GTTAAC 1 cut(s) 3967
HpaII CCGG 7 cut(s) 59, 2502, 3779, 4669, 4817, 5209, 5228
HphI GGTGA 9 cut(s) 560, 1172, 1571, 2023, 2189, 3170, 3332, 4125, 5234
Hpy99I CGWCG 2 cut(s) 1801, 5160
HpyCH4IV ACGT 9 cut(s) 67, 144, 1327, 1906, 2565, 3274, 3586, 3743, 3963
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 1139, 1240, 2023, 2126, 2935, 3812, 4795, 4908
HpySE526I ACGT 9 cut(s) 67, 144, 1327, 1906, 2565, 3274, 3586, 3743, 3963
Hsp92I GRCGYC 1 cut(s) 1783
HspAI GCGC 6 cut(s) 247, 621, 2129, 3630, 3799, 5258
Kpn2I TCCGGA 2 cut(s) 2501, 4816
KpnI GGTACC 1 cut(s) 1991
Ksp22I TGATCA 4 cut(s) 2551, 3391, 3499, 4765
KspAI GTTAAC 1 cut(s) 3967
LmnI GCTCC 8 cut(s) 958, 1240, 1954, 2584, 3310, 3779, 3892, 5223
MabI ACCWGGT 2 cut(s) 5216, 5299
MaeII ACGT 9 cut(s) 67, 144, 1327, 1906, 2565, 3274, 3586, 3743, 3963
MfeI CAATTG 5 cut(s) 576, 1587, 2234, 3829, 3900
MhlI GDGCHC 5 cut(s) 908, 1245, 5228, 5308, 5314
MlyI GAGTC 6 cut(s) 853, 1603, 2701, 3965, 4102, 5195
MmeI TCCRAC 3 cut(s) 498, 1343, 1624
Mph1103I ATGCAT 2 cut(s) 6, 2526
MroI TCCGGA 2 cut(s) 2501, 4816
MroXI GAANNNNTTC 3 cut(s) 705, 1850, 3284
MslI CAYNNNNRTG 9 cut(s) 1764, 2442, 2452, 2844, 3342, 3501, 4086, 4138, 4362
MspA1I CMGCKG 3 cut(s) 1985, 3910, 4902
MspCI CTTAAG 1 cut(s) 190
MspI CCGG 7 cut(s) 59, 2502, 3779, 4669, 4817, 5209, 5228
MspR9I CCNGG 8 cut(s) 60, 1931, 3328, 3779, 5210, 5218, 5228, 5301
MunI CAATTG 5 cut(s) 576, 1587, 2234, 3829, 3900
Mva1269I GAATGC 2 cut(s) 347, 4712
MvaI CCWGG 4 cut(s) 1931, 3328, 5218, 5301
MvnI CGCG 1 cut(s) 2929
MwoI GCNNNNNNNGC 9 cut(s) 10, 1139, 1240, 2023, 2126, 2935, 3812, 4795, 4908
NciI CCSGG 4 cut(s) 60, 3779, 5210, 5228
NcoI CCATGG 1 cut(s) 1039
NdeI CATATG 1 cut(s) 2905
NmuCI GTSAC 3 cut(s) 2191, 3158, 4448
NsbI TGCGCA 3 cut(s) 248, 622, 5259
NsiI ATGCAT 2 cut(s) 6, 2526
NspI RCATGY 3 cut(s) 2441, 3581, 3758
NspV TTCGAA 1 cut(s) 667
OliI CACNNNNGTG 1 cut(s) 4138
PagI TCATGA 3 cut(s) 3496, 3502, 4408
PceI AGGCCT 1 cut(s) 804
PciI ACATGT 3 cut(s) 2437, 3577, 3754
PctI GAATGC 2 cut(s) 347, 4712
PdmI GAANNNNTTC 3 cut(s) 705, 1850, 3284
PflMI CCANNNNNTGG 5 cut(s) 578, 595, 796, 1589, 2688
PfoI TCCNGGA 1 cut(s) 5208
Ple19I CGATCG 2 cut(s) 2215, 5296
PleI GAGTC 6 cut(s) 852, 1602, 2700, 3964, 4102, 5194
PmaCI CACGTG 2 cut(s) 1907, 3587
PmlI CACGTG 2 cut(s) 1907, 3587
PpsI GAGTC 6 cut(s) 852, 1602, 2700, 3964, 4102, 5194
Ppu21I YACGTR 3 cut(s) 1328, 1907, 3587
PpuMI RGGWCCY 1 cut(s) 3552
PscI ACATGT 3 cut(s) 2437, 3577, 3754
PshAI GACNNNNGTC 1 cut(s) 4416
PsiI TTATAA 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 1245
Psp5II RGGWCCY 1 cut(s) 3552
Psp6I CCWGG 4 cut(s) 1929, 3326, 5216, 5299
PspCI CACGTG 2 cut(s) 1907, 3587
PspEI GGTNACC 2 cut(s) 3158, 4549
PspFI CCCAGC 2 cut(s) 2897, 4902
PspGI CCWGG 4 cut(s) 1929, 3326, 5216, 5299
PspPI GGNCC 8 cut(s) 2623, 2708, 2852, 3552, 3781, 3976, 4030, 4713
PspPPI RGGWCCY 1 cut(s) 3552
PsrI GAACNNNNNNTAC 2 cut(s) 3573, 3605
PstI CTGCAG 3 cut(s) 833, 3058, 3209
PstNI CAGNNNCTG 1 cut(s) 595
PvuI CGATCG 2 cut(s) 2215, 5296
PvuII CAGCTG 2 cut(s) 3910, 4902
RseI CAYNNNNRTG 9 cut(s) 1764, 2442, 2452, 2844, 3342, 3501, 4086, 4138, 4362
Rsr2I CGGWCCG 1 cut(s) 2623
RsrII CGGWCCG 1 cut(s) 2623
SacI GAGCTC 1 cut(s) 1245
Sau96I GGNCC 8 cut(s) 2623, 2708, 2852, 3552, 3781, 3976, 4030, 4713
ScaI AGTACT 2 cut(s) 3862, 3873
SchI GAGTC 6 cut(s) 853, 1603, 2701, 3965, 4102, 5195
ScrFI CCNGG 8 cut(s) 60, 1931, 3328, 3779, 5210, 5218, 5228, 5301
SduI GDGCHC 5 cut(s) 908, 1245, 5228, 5308, 5314
SexAI ACCWGGT 2 cut(s) 5216, 5299
SfcI CTRYAG 6 cut(s) 829, 1840, 2399, 3054, 3205, 4126
SfuI TTCGAA 1 cut(s) 667
SinI GGWCC 6 cut(s) 2623, 2852, 3552, 3976, 4030, 4713
SmiMI CAYNNNNRTG 9 cut(s) 1764, 2442, 2452, 2844, 3342, 3501, 4086, 4138, 4362
SmlI CTYRAG 9 cut(s) 190, 524, 655, 907, 1013, 3530, 4220, 4453, 4517
SmoI CTYRAG 9 cut(s) 190, 524, 655, 907, 1013, 3530, 4220, 4453, 4517
SpeI ACTAGT 1 cut(s) 3868
SseBI AGGCCT 1 cut(s) 804
SspI AATATT 3 cut(s) 485, 1683, 4252
SstI GAGCTC 1 cut(s) 1245
StuI AGGCCT 1 cut(s) 804
StyD4I CCNGG 8 cut(s) 58, 1929, 3326, 3777, 5208, 5216, 5226, 5299
StyI CCWWGG 4 cut(s) 1039, 1114, 4461, 4803
TaiI ACGT 9 cut(s) 70, 147, 1330, 1909, 2568, 3277, 3589, 3746, 3966
TaqII GACCGA 2 cut(s) 2640, 4047
TatI WGTACW 7 cut(s) 548, 2142, 2669, 3437, 3860, 3871, 4090
TauI GCSGC 2 cut(s) 414, 828
TseFI GTSAC 3 cut(s) 2191, 3158, 4448
Tsp45I GTSAC 3 cut(s) 2191, 3158, 4448
TspGWI ACGGA 4 cut(s) 2119, 3537, 4592, 4733
Van91I CCANNNNNTGG 5 cut(s) 578, 595, 796, 1589, 2688
Vha464I CTTAAG 1 cut(s) 190
VpaK11BI GGWCC 6 cut(s) 2623, 2852, 3552, 3976, 4030, 4713
XbaI TCTAGA 3 cut(s) 349, 628, 1639
XceI RCATGY 3 cut(s) 2441, 3581, 3758
XcmI CCANNNNNNNNNTGG 3 cut(s) 1046, 2005, 4015
XmaJI CCTAGG 1 cut(s) 1114
XmiI GTMKAC 1 cut(s) 2335
XmnI GAANNNNTTC 3 cut(s) 705, 1850, 3284
ZrmI AGTACT 2 cut(s) 3862, 3873
Zsp2I ATGCAT 2 cut(s) 6, 2526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.