Rroxscaffold_7G00216560

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000307
Physical Location & Seq
Forward (+)
67201359 .. 67212961
11603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00216560.1

Sequence Viewer

Length: 6699 bp
ATGTTTACCGTCCTCCTTTTCCTCATTCTCCTTGTTTCTCTTCTTCTTCAGTTTCTTAATTATCTTCTACCACCACCTGCCCAATCTCCAGCAGATTCCCCGAACATCCAAACACCTGCATCTCCTTCAAACCCTAAGTCAGAACATAAGGAAGTTCCAGCGGATTCCACAAACATCCAAATACCTGCATCTCCTTCGAACCCTAAATCAGAACATGATGAAGTTCCAGCGGCTTCCACAAACATCCAAATACTTGCATCTCCTTCAAACCCTAAATCAGAACATGAGGAAGTTCCAGCGGATTCCCCGAACATCCAAATACCTGCATCTACTTCAACCCCTAAATTAGAACATGAGGAAGTTCCAGCAGACTCCACAAACACCCAAACAGTACTTTCATCTCTTTCAGCCCCAATGGAACATGAGAAAGTTCCTCCAGCAGCTTCCTCTTCTTCGGGCTCAACATTGGAACATGATGTTGTATCCCATGATGTCTTCCTCAGTTTTAGAGGTGAAGACACCCGCTATGCTTTTACTGACCATTTATATCATGCTATGAGGGGGCAAGGAATTGATGCATTCAGAGATACAGAAAAACTCGAGAGGGGACATTCAATTTCACCCGATCTCTTGAAAGCAATAAAAGAATCCCGAATTGCAGTCATCATTCTTTCATCTAACTATGCTACTTCAACATGGTGTTTGGAGGAGCTTGCACAGATTATTGAATGCAAAGAGTCGAAGGGGTTGAAAGTTTTACCAGTTTTCTATCACGTCGAACCATCAGAAGTAAGGAAGCAAACAGGAAATTTTCACCAGGCGTTTTGCAAGCATGAAGAAACTTTCGAAGACAACCCGGAGAAGGTGAAGAAGTGGAGAGAAGCTTTAAACCAAGTAGCCAATCTCTCCGGATGGACTTTGAAAGAAAGAGGAGAATCTGAAGTTATTCAGACAATAACTACAGAGATATTGAACACGTTGAATAACATGTGCTCAAGTGATAACAATGATTTAATTGGAATGGATGCTCGTATACAGAAACTAATGTGTTACTTGGATCTGGGGTCGAATGATGTTCGCCCCATAGGGATTGCCGGGATGGGCGGCATCGGAAAATCGACTCTGGCAAAGGCAGTTTTTGAAAAGATCCGTCACCAATTTGAATCTAGCTGCTTTATTAATATAGCTGACGTTAGAGGGAAATCCCTAGTTAAATTGCAGAAACTTCTCTGTCAAAATTTGCAGCTGGACAACCATGAAAGTATACAGAATGATTCTATGGGGATCAATTTATTAAGGAAACGATTAAGTCATAAAAGGGTGCTTATCATTCTAGATGATGTTGATCATACTAGTCAACTAGAAGCTCTCGTTGCCAAAGAAGACAAAGGAAAAAAGCCTTGTTTGGGTCCAGGGAGCAGAGTCATTATAACAACTAGAGATACACACAAGCTGAAGGAATTTGGATTACTGGAAGATGCTATATATGAGGCTGAGAAGTTGACTGATAAGGAAGCTCTTCAGCTCTTATGTCAGAAAGCTTTCAAGGAAAACCACGCTCCAGATGAATATAGGGAACTGTCCAATAGTTTTGTAAAATATTGCAGTGGCCTTCCTTTAGCTCTCAAAGTTCTGGGTTCTCACTTGTGTACGAGAACGGTTGATGAATGGTCAGCAGAATTGGCTAGACTTGATGAAGATCCCCACAAAGATATTTTTTCTATTCTTCAATTAAGTTTTGATGGATTGAAGAATACAGAGAAAGATATCTTTTTGGACATAGCATGTTTATTCAGAGGCGAGAGTCAATATCGTGTAAGAAAGATTTTAGAGAGCTGTGACTTTCATCCTGGTATCGGAATATCTGTCCTCGTTGAAAAACATTTAATTAATGTTGAGGAAGATAAACTGTGGATGCATGATTTGCTACAGCAAATGGGTTGGCACATTGTTCGCCAAGAATCTCCTGAGCCGGGAAAACGAAGCAGGTTGTGGCTTAATGAGAATTCCCATGAGTATCAACACAGCCGCCCGTGGTTTGATGAAGATGCTCGTGATGTAGTCACCGAGAATCTGGGAACATATGCTGTCCAAGGGGTATATTTGAGCGTGCTGGAAAAAGAAAAGATACAATTGAAAGCTGACCCCTTCTTAAATATGCGCAAGCTAAGATTGTTGAAGATTTGTAATGTCAACTTGGTGGATGTGCGATTTGAATATCTGTCTAACAAGTTACGACTTTTGGAATGGCATGAATGTCCTCTAGAATCTCTGCCATCCAGTTTTCGACCAGATAAACTCGTCGAATTGAAACTGCCCAACAGCCGCATTGAAACACTGTGGAATCAAAGACTCTGTCTGGAGAAGCTAGTACTGATGGATCTTTCCTACTGCCAATACTTGGTCAAGACCCCGGACTTCAGTAATTTCCCAAATCTTGAACAGTTGACTTTTGCAGGTTGTGAAAATTTATCAGATGTTCACCCAACACTTTGGAGGCTCAAAAATTTGAAGGTTGCAATTTTTGAAGATTGCACGAGTCTAGAGAACCTTCCCAACTCCATCAGCTTGCAATCTCTCGCAATTCTTGTTCTTTCGGGATGTTCAAAACTAGATGAGTTTCCGGAGATAGTGGGAAACAGGGAAACTCTGGCAGCACTCTATTTAGATGGAACAGCTATAAGAGAGCTACCAACCACAATCGGGTGCTTGAGTGGCCTAATTAGATTAAGTCTATGTGCCTGCAAGAACCTTGTGGGTCTTCCAAGCTTCATTTGCAGTTTGAAGTCTCTGAGATATCTGCTTCTGTCAAAATCCTCACGCATTGACCAACTTCCAGAAGACATAGGGAGCTTGGAAAATTTGGAGAAGCTTGATGCTTGTCGCACTGCTATAAGAAAAGTGCCTTCGTCCATTGTATTTCTGAAGAATCTGAAATCATTATGTTTCCAGGGATGTAGGCAGGTGCCCGGGGAATGCTCTGATAAGTCCTTGGGTTTGCAATTGCCAAAGTCGTTCTCCGGTTTGAGCTCATTGGAAACACTATATTTAGGTCAGTGCGATCTTGCTGAAGGAGCGATCCCAGATGACATTGGCTGCTTATCCTCCCTGAAACATATAAGGTTGGATGAAAACAACTTTGTGAGTATACCTGAAAGCATCTCTCAACTTCCTAAGCTTAGAGAAATTCACATCCCAAATTGCAGCAAGCTTCAGTCTTTGCCAAATCGTCTTCCAGAAGATGTTAATGTAGAGGCTAGTGGATGTCCCCTGCTGACAAACTATCCAAACCGGCTTAACATACAGCTTTCTGATGAGGGGATCCGTCGCATAAATTGTGGAAATTCAAAGCATGCAAGCTGTCCACTTCCAATTCCTGAAGAACAAATTGAAGAATACTTACCCAAGTACCTTGAGGATGAAATTGTTCTTCAGCAGCACCTGCAGCTTCGTTACGTCCATACAGAAATTCCTGATTGGTGTCGTCACAAGAGTAGTGAGACTTCTGTCGCTATCGTAGCCATGCAAGTCTATGATCCTGAAATTATTAAGAGCTTGGGAGTAGCCCTGTTTGTTGTTTTTGAAATCTTTGAGAGTGGTCACAATTTTGATAAGAGTTGGGAGTTGCAACAGACATTTTGTACTTTTAAAGATGACCATGGACTTGAACATATCTATATCTTTAAAGAATTTGAAAATTTCTTGGCTGGGACATATGTATTATTTTGCTATGAGCCTCTAAAGTACTTTCAAATTGCTGGAACGTTGAATAAAGCAAGTCGATTTGAGGCTTCAGTTTCAACAGAGAGACCAGATCTGGTGGTCAAGTCTTGTGGGATGCAACTAATCTTTCAGGAAGAAGCTAAGGAGTTTGCTCAAAAATTAAGTAAGGCTTGTAATCCTCAGACGGATTTCAAATTTGGTACACATTGTAAGGAATTGATAAACTCCAAGGGGAAGGAAAGCTCCGGTAATATAATGGCACCAAATGAAGAAGAAAAGGTCAGCTGGAAATTTGATTTTAACATGCACCCGGGAGAAGATCCACAGTTGTTGCTTTCAAAAGTCTATGAGGATTTCAATGGGGATATCATATACAATGCAACTTACCCTCATTGCGCTGAACTTTTGGAGTGGTTCGGTGATCATCATCTCAGTAGGGCCCCTTCAGTGAAAATCCCGTTACCACCATCTCAATCTAGCGATGGGAGTTGGATAGGATTGGCTCTATATGCACGTTTTTCAGACCTCGAGAATCAAACTACCTCCATAGACAAAGTGAACCCAGAAATTCCTGCGCTTGATTGTCATTTGGAAACTGAGAACGGTACCCTCGAACATCTTCACCACTATCGACCAACCAATGAAGAATTCAAGCAGTTATGTCCTACAGAATTCATTTGGCTATCCTATATACCACGTCAGGGGTTTCTAGATCAGTTAAATGAGTGTGACCTCTTGGAGGCTTCATTTGCCAGCGAAAGCCGAGGATTGTTTGCTGAAGAATGTGGTCTCCGTCTTGTCTACCAGCATGATGAGCAAGAGTTTAAGAATCTGTGTACCTTCATGAACTCGTTTCTGGACAAAAGATCTGAAAGGTTGGAAGAATATGAGGCTCCCAAGAAGGGGGACGTGGCACTGTGCGACTTTCCTCTAGACCTATCTGATGATCAGAGATGGGTCGGTTTAGAACTATTTGTTATGGTTTCGAGTGGTTACAGTATCAATGTTGAATCCTTACTTGATGTTGATTTGTGTTCCGATAAAATTAGCACAATAAGCACATCTGTAAAGATAAACACTTCTCTGGGCTCAGATCAACTTGTTGTAATCCATATACCACGCGTGCGTTTTCCGGAGCAGTTGAATCAATGTCATGGTATCAGCGCATTGTTTAGAACCGCTACTCCGGATATGAGGGTTCAAATGTGTGGAAGCCGTTTACTATACGAGCAAGATTTGGAAGACTTGATCCGTGCAATAACCGTGTGCGCATTAGGCAGTCAAGATGTCATTGATTCTCTTTGTCAGCACATCCAACAATTACTTGGAAGTTGCATGCCGGTGGAACCTCTTGATCATATCGCAGCAGTTGAAACCGCTGAGCAGAATTCATTGGAAGGAGAAAACGACGTTGGTCGCAAAAATGATCATTGCTCCTCTGAAAGGGAGGACTTGATTCAAGACCCCAGAGAGCAAGTCTTCACTTCAATATTGCACAACTACAGATCTTATCTCCAACAAAAGCATGGTTGTATAGAGTCTGAAAGAATTATATCCCATTCCGAAGGCTATATTTTCGACAATGGGAATCCAAAGCCGGTGTTTGCCACGCATCATTTACATGTAATGGTTGAGACTCGACTCTATGGGAGCCTTAGCTCAGAAGATTGGAAACGATCTCTCGAGTCATTGCTTCAGTATTTCAAGGTGGCCACTCTCAGTGTAAAAGGACAAAGGATTGCTGTTTTAAATCCGTTCACTCCTTCTTCAGTAAACAACTTTTGTTTCCCTCGAAAGGAACTTCCAGGATGGTTCTGGCAACTAAGAGTGCCCAGGATTAGATTGACCATATGCCCACTTGTATGTGATGATGAGAATTGGTTGGGACTTGCTATATGTGCTACATTTTCAGTCCACGAGCATCCAAGTGCTGTCCTTGACAATCGGGCCACAAGGACTTTCAACGTTATGTGTCATTTGGCAGCGTTAGATGGACGATGTTTCAGTCATGCCCCGGTGTTTACCATAACAAAAGAGAGATTCAAGTGGCTACATCTTCGTGGATTTATGTGGCTAACCTACATACCTCGTTCTTATCTTACAGAGTTCAGAAAAGTAGGTGTTGCAGAAGCTAGAATTTATAATGATTGCCCAGGATTAATGATGAAAGAGTGGAGTATCCGTCCCTTGTTCAAACAGGATGTGAAGGAATTTAATAAAGCAATAGTCCACTGCTGGACTTCGTTCTTCGACAATTTGGATCTCATCCGTCAGTCTGTCGAAGCAGATGAAATTGAACGGGCACATTATTTTGATAAAGGGGAAACCAGTAAAATCTCGATGCCTAAAGGACATGTGAAGGATTTCGATCGGGACATCATATACAATGCAACTTACCCTCATTGCGCTAAATTTTTGGAGTGGTTCGGTGATCATCATCTCAGTAGGGGTCCTTCAGTGAAAATCCCGTTACCACCATCTCTATCTACTGATGGGAATTGGATAGGATTGGCTCTATGTGCACGTTTTTCAGATCTCGAGAATCAAACTACCTCCATAGACAAAGTGAATCCAGAAATCGCTGAGCTTGTTTGTCATTTGGAAACTGAGAACAGTGGTTTCAAACATCTTCACCACTATCGACCAACCAATGAAGAATTCAAGTGGTTATGTCCTACAGAATTCATTTGGCTATCTTATATACCACGTCAGGGGTATCTAGATCAGTTAAATGAGTATGACCTCTTGGAGGCTTCATTTGCTAGCGAAAGCCGAGGGTTGTTTGCTGAAGTGTGTGTTCTCCGTCTTGTCTACCAGCATGATGAGCAAGAGTTTAAGGATCTGTGTAACTTCATGAACTCGTTGCTGGACCAAGAGGCTCCCAAGAAGGGTAAACAGGAACCTGATATTGAATGGGGATCTAATGCAATGTGGAATCTGGAGATCTGCGACGACCCAAAATGGAGACATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

2232

Amino Acids

253.91

Weight (kDa)

5.37

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 163 - 337 7.2e-56 TIR domain
TIR_2 PF13676 165 - 275 1.2e-15 TIR domain
NB-ARC PF00931 347 - 518 9e-22 NB-ARC domain
WHD_ROQ1 PF23282 586 - 655 8.3e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 826 - 931 1.3e-07 Leucine-rich repeat region
LRR_13 PF23286 857 - 928 2.1e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 924 - 991 2.5e-13 Leucine-rich repeat region
LRR_13 PF23286 940 - 1076 9.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1430, 5865
AarI CACCTGC 4 cut(s) 85, 124, 2953, 3450
Acc16I TGCGCA 2 cut(s) 2162, 4993
Acc36I ACCTGC 8 cut(s) 85, 124, 193, 331, 1977, 2447, 2953, 3450
Acc65I GGTACC 1 cut(s) 4328
AccB1I GGYRCC 3 cut(s) 2965, 3982, 4328
AccB7I CCANNNNNTGG 1 cut(s) 2401
AccI GTMKAC 5 cut(s) 1033, 1264, 3148, 4524, 6534
AccII CGCG 1 cut(s) 4845
AccIII TCCGGA 4 cut(s) 908, 2623, 4855, 4909
AciI CCGC 9 cut(s) 161, 230, 299, 523, 1104, 2029, 2326, 4902, 5100
AclI AACGTT 2 cut(s) 3764, 5688
AcoI YGGCCR 1 cut(s) 5433
AfaI GTAC 9 cut(s) 393, 1651, 2373, 3410, 3643, 3746, 3925, 4330, 4561
AflIII ACRYGT 5 cut(s) 975, 987, 4843, 5344, 6076
AhlI ACTAGT 1 cut(s) 1352
AjiI CACGTC 4 cut(s) 775, 4421, 4633, 6431
AjnI CCWGG 7 cut(s) 816, 1411, 1849, 2949, 5527, 5555, 5875
AjuI GAANNNNNNNTTGG 6 cut(s) 4355, 4387, 5118, 5150, 6365, 6397
AloI GAACNNNNNNTCC 2 cut(s) 4891, 4923
Alw21I GWGCWC 3 cut(s) 995, 3032, 6247
Alw26I GTCTC 6 cut(s) 2793, 3494, 3802, 4517, 5351, 6682
Alw44I GTGCAC 1 cut(s) 6243
AlwNI CAGNNNCTG 1 cut(s) 3442
Ama87I CYCGRG 6 cut(s) 599, 2969, 4033, 4250, 5405, 6260
Aor13HI TCCGGA 4 cut(s) 908, 2623, 4855, 4909
AoxI GGCC 5 cut(s) 1609, 2716, 4161, 5433, 5670
ApaI GGGCCC 1 cut(s) 4165
ApaLI GTGCAC 1 cut(s) 6243
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 2341, 2373
AseI ATTAAT 3 cut(s) 1179, 1890, 5882
Asp700I GAANNNNTTC 6 cut(s) 945, 1518, 1541, 3426, 4341, 6086
Asp718I GGTACC 1 cut(s) 4328
AspLEI GCGC 6 cut(s) 2163, 4121, 4300, 4889, 4994, 6131
AspS9I GGNCC 6 cut(s) 1409, 4161, 4162, 5670, 6173, 6592
AsuC2I CCSGG 9 cut(s) 857, 1096, 1974, 2414, 2970, 2971, 4034, 4035, 5738
AsuII TTCGAA 2 cut(s) 197, 846
AsuNHI GCTAGC 1 cut(s) 6485
AvaI CYCGRG 6 cut(s) 599, 2969, 4033, 4250, 5405, 6260
AvaII GGWCC 3 cut(s) 1409, 6173, 6592
BaeGI GKGCMC 5 cut(s) 2970, 4165, 5556, 6028, 6247
BaeI ACNNNNGTAYC 2 cut(s) 3392, 3425
BalI TGGCCA 1 cut(s) 5435
BamHI GGATCC 1 cut(s) 3321
BanI GGYRCC 3 cut(s) 2965, 3982, 4328
BanII GRGCYC 4 cut(s) 461, 3032, 4165, 4814
BarI GAAGNNNNNNTAC 8 cut(s) 3384, 3416, 3487, 3519, 4150, 4182, 6160, 6192
BauI CACGAG 3 cut(s) 2052, 2536, 5639
BbsI GAAGAC 9 cut(s) 487, 522, 855, 1389, 2753, 2847, 3224, 4971, 5194
Bbv12I GWGCWC 3 cut(s) 995, 3032, 6247
BceAI ACGGC 1 cut(s) 4923
BcgI CGANNNNNNTGC 4 cut(s) 177, 211, 1934, 1968
BciT130I CCWGG 7 cut(s) 818, 1413, 1851, 2951, 5529, 5557, 5877
BciVI GTATCC 2 cut(s) 493, 5912
BclI TGATCA 6 cut(s) 1345, 4144, 4669, 5077, 5149, 6154
BcnI CCSGG 9 cut(s) 857, 1096, 1974, 2414, 2970, 2971, 4034, 4035, 5738
BcoDI GTCTC 6 cut(s) 2793, 3494, 3802, 4517, 5351, 6682
BcuI ACTAGT 1 cut(s) 1352
BfmI CTRYAG 6 cut(s) 960, 1928, 3443, 4389, 5224, 6399
BfuAI ACCTGC 8 cut(s) 85, 124, 193, 331, 1977, 2447, 2953, 3450
BfuI GTATCC 2 cut(s) 493, 5912
BglII AGATCT 5 cut(s) 3814, 4589, 5228, 6256, 6666
BlpI GCTNAGC 2 cut(s) 5103, 6306
BmcAI AGTACT 3 cut(s) 393, 2373, 3746
Bme18I GGWCC 3 cut(s) 1409, 6173, 6592
BmeT110I CYCGRG 6 cut(s) 599, 2969, 4033, 4250, 5405, 6260
BmgBI CACGTC 4 cut(s) 775, 4421, 4633, 6431
BmgT120I GGNCC 6 cut(s) 1409, 4161, 4162, 5670, 6173, 6592
BmtI GCTAGC 1 cut(s) 6489
BoxI GACNNNNGTC 2 cut(s) 3506, 5136
BpiI GAAGAC 9 cut(s) 487, 522, 855, 1389, 2753, 2847, 3224, 4971, 5194
BpmI CTGGAG 5 cut(s) 72, 420, 1545, 2381, 6683
Bpu10I CCTNAGC 4 cut(s) 1968, 3174, 3864, 5378
Bpu1102I GCTNAGC 2 cut(s) 5103, 6306
Bpu14I TTCGAA 2 cut(s) 197, 846
BpuEI CTTGAG 3 cut(s) 979, 2731, 3434
BpuMI CCSGG 9 cut(s) 857, 1096, 1974, 2414, 2970, 2971, 4034, 4035, 5738
BsaBI GATNNNNATC 5 cut(s) 1344, 1698, 4149, 6090, 6159
BsaI GGTCTC 2 cut(s) 3802, 4517
BsaWI WCCGGW 6 cut(s) 908, 2623, 3020, 3968, 4855, 4909
BsaXI ACNNNNNCTCC 4 cut(s) 4891, 4921, 5890, 5920
Bse118I RCCGGY 3 cut(s) 3291, 5062, 5320
Bse1I ACTGG 4 cut(s) 761, 1476, 2280, 6051
Bse3DI GCAATG 5 cut(s) 4114, 5152, 5411, 6124, 6657
Bse8I GATNNNNATC 5 cut(s) 1344, 1698, 4149, 6090, 6159
BseAI TCCGGA 4 cut(s) 908, 2623, 4855, 4909
BseBI CCWGG 7 cut(s) 818, 1413, 1851, 2951, 5529, 5557, 5877
BseJI GATNNNNATC 5 cut(s) 1344, 1698, 4149, 6090, 6159
BseMI GCAATG 5 cut(s) 4114, 5152, 5411, 6124, 6657
BseNI ACTGG 4 cut(s) 761, 1476, 2280, 6051
BseRI GAGGAG 3 cut(s) 722, 945, 5149
BseSI GKGCMC 5 cut(s) 2970, 4165, 5556, 6028, 6247
BseYI CCCAGC 1 cut(s) 3707
Bsh1236I CGCG 1 cut(s) 4845
Bsh1285I CGRYCG 1 cut(s) 6094
BshFI GGCC 5 cut(s) 1611, 2718, 4163, 5435, 5672
BshNI GGYRCC 3 cut(s) 2965, 3982, 4328
BsiEI CGRYCG 1 cut(s) 6094
BsiHKAI GWGCWC 3 cut(s) 995, 3032, 6247
BsiHKCI CYCGRG 6 cut(s) 599, 2969, 4033, 4250, 5405, 6260
BslFI GGGAC 7 cut(s) 621, 3252, 3724, 4643, 5622, 5892, 6110
BsmAI GTCTC 6 cut(s) 2793, 3494, 3802, 4517, 5351, 6682
BsmFI GGGAC 7 cut(s) 621, 3252, 3724, 4643, 5622, 5892, 6110
BsmI GAATGC 3 cut(s) 578, 734, 2981
BsnI GGCC 5 cut(s) 1611, 2718, 4163, 5435, 5672
Bso31I GGTCTC 2 cut(s) 3802, 4517
BsoBI CYCGRG 6 cut(s) 599, 2969, 4033, 4250, 5405, 6260
Bsp119I TTCGAA 2 cut(s) 197, 846
Bsp120I GGGCCC 1 cut(s) 4161
Bsp1286I GDGCHC 9 cut(s) 461, 995, 2970, 3032, 4165, 4814, 5556, 6028, 6247
Bsp13I TCCGGA 4 cut(s) 908, 2623, 4855, 4909
Bsp1720I GCTNAGC 2 cut(s) 5103, 6306
Bsp19I CCATGG 1 cut(s) 3658
BspACI CCGC 9 cut(s) 161, 230, 299, 523, 1104, 2029, 2326, 4902, 5100
BspANI GGCC 5 cut(s) 1611, 2718, 4163, 5435, 5672
BspEI TCCGGA 4 cut(s) 908, 2623, 4855, 4909
BspFNI CGCG 1 cut(s) 4845
BspHI TCATGA 2 cut(s) 4566, 6576
BspMAI CTGCAG 1 cut(s) 3447
BspMI ACCTGC 8 cut(s) 85, 124, 193, 331, 1977, 2447, 2953, 3450
BspOI GCTAGC 1 cut(s) 6489
BspQI GCTCTTC 1 cut(s) 1524
BspT104I TTCGAA 2 cut(s) 197, 846
BspT107I GGYRCC 3 cut(s) 2965, 3982, 4328
BspTNI GGTCTC 2 cut(s) 3802, 4517
BsrDI GCAATG 5 cut(s) 4114, 5152, 5411, 6124, 6657
BsrFI RCCGGY 3 cut(s) 3291, 5062, 5320
BsrI ACTGG 4 cut(s) 761, 1476, 2280, 6051
BssAI RCCGGY 3 cut(s) 3291, 5062, 5320
BssNAI GTATAC 3 cut(s) 1034, 1265, 3149
BssSI CACGAG 3 cut(s) 2052, 2536, 5639
BssT1I CCWWGG 4 cut(s) 2092, 2991, 3658, 3951
Bst1107I GTATAC 3 cut(s) 1034, 1265, 3149
Bst2BI CACGAG 3 cut(s) 2052, 2536, 5639
Bst2UI CCWGG 7 cut(s) 818, 1413, 1851, 2951, 5529, 5557, 5877
Bst6I CTCTTC 3 cut(s) 45, 454, 1524
BstAPI GCANNNNNTGC 2 cut(s) 1924, 3442
BstBI TTCGAA 2 cut(s) 197, 846
BstDSI CCRYGG 2 cut(s) 2033, 3658
BstENI CCTNNNNNAGG 3 cut(s) 4460, 5164, 6470
BstFNI CGCG 1 cut(s) 4845
BstHHI GCGC 6 cut(s) 2163, 4121, 4300, 4889, 4994, 6131
BstMAI GTCTC 6 cut(s) 2793, 3494, 3802, 4517, 5351, 6682
BstMCI CGRYCG 1 cut(s) 6094
BstNI CCWGG 7 cut(s) 818, 1413, 1851, 2951, 5529, 5557, 5877
BstNSI RCATGY 7 cut(s) 991, 1788, 3356, 4030, 5062, 5348, 6080
BstPAI GACNNNNGTC 2 cut(s) 3506, 5136
BstSFI CTRYAG 6 cut(s) 960, 1928, 3443, 4389, 5224, 6399
BstSLI GKGCMC 5 cut(s) 2970, 4165, 5556, 6028, 6247
BstUI CGCG 1 cut(s) 4845
BstV2I GAAGAC 9 cut(s) 487, 522, 855, 1389, 2753, 2847, 3224, 4971, 5194
BstXI CCANNNNNNTGG 1 cut(s) 2493
BstZ17I GTATAC 3 cut(s) 1034, 1265, 3149
BsuI GTATCC 2 cut(s) 493, 5912
BsuRI GGCC 5 cut(s) 1611, 2718, 4163, 5435, 5672
BtgI CCRYGG 2 cut(s) 2033, 3658
BtgZI GCGATG 1 cut(s) 4218
BtrI CACGTC 4 cut(s) 775, 4421, 4633, 6431
BtsI GCAGTG 3 cut(s) 1612, 2886, 5953
BveI ACCTGC 8 cut(s) 85, 124, 193, 331, 1977, 2447, 2953, 3450
CaiI CAGNNNCTG 1 cut(s) 3442
CciI TCATGA 2 cut(s) 4566, 6576
CfoI GCGC 6 cut(s) 2163, 4121, 4300, 4889, 4994, 6131
Cfr10I RCCGGY 3 cut(s) 3291, 5062, 5320
Cfr13I GGNCC 6 cut(s) 1409, 4161, 4162, 5670, 6173, 6592
Cfr9I CCCGGG 2 cut(s) 2969, 4033
Csp6I GTAC 9 cut(s) 392, 1650, 2372, 3409, 3642, 3745, 3924, 4329, 4560
CspCI CAANNNNNGTGG 4 cut(s) 3814, 3849, 4035, 4070
CviQI GTAC 9 cut(s) 392, 1650, 2372, 3409, 3642, 3745, 3924, 4329, 4560
DraI TTTAAA 4 cut(s) 888, 3649, 3685, 5472
EaeI YGGCCR 1 cut(s) 5433
Eam1104I CTCTTC 3 cut(s) 45, 454, 1524
EarI CTCTTC 3 cut(s) 45, 454, 1524
Ecl136II GAGCTC 1 cut(s) 3030
Eco130I CCWWGG 4 cut(s) 2092, 2991, 3658, 3951
Eco24I GRGCYC 4 cut(s) 461, 3032, 4165, 4814
Eco31I GGTCTC 2 cut(s) 3802, 4517
Eco32I GATATC 3 cut(s) 1768, 2798, 4090
Eco47I GGWCC 3 cut(s) 1409, 6173, 6592
Eco53kI GAGCTC 1 cut(s) 3030
Eco88I CYCGRG 6 cut(s) 599, 2969, 4033, 4250, 5405, 6260
EcoICRI GAGCTC 1 cut(s) 3030
EcoNI CCTNNNNNAGG 3 cut(s) 4460, 5164, 6470
EcoO109I RGGNCCY 3 cut(s) 4161, 4162, 6173
EcoRI GAATTC 6 cut(s) 2005, 4370, 4394, 5110, 6380, 6404
EcoRII CCWGG 7 cut(s) 816, 1411, 1849, 2949, 5527, 5555, 5875
EcoRV GATATC 3 cut(s) 1768, 2798, 4090
EcoT14I CCWWGG 4 cut(s) 2092, 2991, 3658, 3951
EcoT22I ATGCAT 2 cut(s) 580, 1920
EcoT38I GRGCYC 4 cut(s) 461, 3032, 4165, 4814
ErhI CCWWGG 4 cut(s) 2092, 2991, 3658, 3951
FalI AAGNNNNNCTT 2 cut(s) 3877, 3909
FaqI GGGAC 7 cut(s) 621, 3252, 3724, 4643, 5622, 5892, 6110
FauI CCCGC 1 cut(s) 530
FauNDI CATATG 3 cut(s) 2083, 3715, 5573
FbaI TGATCA 6 cut(s) 1345, 4144, 4669, 5077, 5149, 6154
FblI GTMKAC 5 cut(s) 1033, 1264, 3148, 4524, 6534
FriOI GRGCYC 4 cut(s) 461, 3032, 4165, 4814
FspAI RTGCGCAY 1 cut(s) 4993
FspI TGCGCA 2 cut(s) 2162, 4993
GlaI GCGC 6 cut(s) 2162, 4120, 4299, 4888, 4993, 6130
GsaI CCCAGC 1 cut(s) 3711
GsuI CTGGAG 5 cut(s) 72, 420, 1545, 2381, 6683
HaeIII GGCC 5 cut(s) 1611, 2718, 4163, 5435, 5672
HhaI GCGC 6 cut(s) 2163, 4121, 4300, 4889, 4994, 6131
Hin6I GCGC 6 cut(s) 2161, 4119, 4298, 4887, 4992, 6129
HinP1I GCGC 6 cut(s) 2161, 4119, 4298, 4887, 4992, 6129
HincII GTYRAC 4 cut(s) 1358, 1503, 2194, 2448
HindII GTYRAC 4 cut(s) 1358, 1503, 2194, 2448
HindIII AAGCTT 6 cut(s) 882, 1539, 2767, 2870, 3176, 3209
Hpy99I CGWCG 5 cut(s) 779, 2306, 3330, 5135, 6677
HspAI GCGC 6 cut(s) 2161, 4119, 4298, 4887, 4992, 6129
Kpn2I TCCGGA 4 cut(s) 908, 2623, 4855, 4909
KpnI GGTACC 1 cut(s) 4332
Ksp22I TGATCA 6 cut(s) 1345, 4144, 4669, 5077, 5149, 6154
LguI GCTCTTC 1 cut(s) 1524
MfeI CAATTG 2 cut(s) 2132, 3002
MhlI GDGCHC 9 cut(s) 461, 995, 2970, 3032, 4165, 4814, 5556, 6028, 6247
MlsI TGGCCA 1 cut(s) 5435
MluI ACGCGT 1 cut(s) 4843
MluNI TGGCCA 1 cut(s) 5435
MmeI TCCRAC 5 cut(s) 3105, 4193, 4581, 5062, 5263
Mox20I TGGCCA 1 cut(s) 5435
Mph1103I ATGCAT 2 cut(s) 580, 1920
MroI TCCGGA 4 cut(s) 908, 2623, 4855, 4909
MroXI GAANNNNTTC 6 cut(s) 945, 1518, 1541, 3426, 4341, 6086
MscI TGGCCA 1 cut(s) 5435
MslI CAYNNNNRTG 5 cut(s) 5063, 5343, 5584, 5649, 5781
Msp20I TGGCCA 1 cut(s) 5435
MspA1I CMGCKG 6 cut(s) 161, 230, 299, 1246, 4008, 5102
MunI CAATTG 2 cut(s) 2132, 3002
Mva1269I GAATGC 3 cut(s) 578, 734, 2981
MvaI CCWGG 7 cut(s) 818, 1413, 1851, 2951, 5529, 5557, 5877
MvnI CGCG 1 cut(s) 4845
NciI CCSGG 9 cut(s) 857, 1096, 1974, 2414, 2970, 2971, 4034, 4035, 5738
NcoI CCATGG 1 cut(s) 3658
NdeI CATATG 3 cut(s) 2083, 3715, 5573
NheI GCTAGC 1 cut(s) 6485
NmeAIII GCCGAG 2 cut(s) 4511, 6521
NmuCI GTSAC 6 cut(s) 1151, 1838, 2062, 3485, 3599, 4451
NsbI TGCGCA 2 cut(s) 2162, 4993
NsiI ATGCAT 2 cut(s) 580, 1920
NspI RCATGY 7 cut(s) 991, 1788, 3356, 4030, 5062, 5348, 6080
NspV TTCGAA 2 cut(s) 197, 846
PacI TTAATTAA 1 cut(s) 1890
PaeI GCATGC 2 cut(s) 3356, 5062
PaeR7I CTCGAG 4 cut(s) 599, 4250, 5405, 6260
PagI TCATGA 2 cut(s) 4566, 6576
PaqCI CACCTGC 4 cut(s) 85, 124, 2953, 3450
PciI ACATGT 3 cut(s) 987, 5344, 6076
PciSI GCTCTTC 1 cut(s) 1524
PcsI WCGNNNNNNNCGW 1 cut(s) 4332
PctI GAATGC 3 cut(s) 578, 734, 2981
PdmI GAANNNNTTC 6 cut(s) 945, 1518, 1541, 3426, 4341, 6086
PflFI GACNNNGTC 1 cut(s) 2355
PflMI CCANNNNNTGG 1 cut(s) 2401
PfoI TCCNGGA 1 cut(s) 5527
Ple19I CGATCG 1 cut(s) 6094
PpuMI RGGWCCY 1 cut(s) 6173
PscI ACATGT 3 cut(s) 987, 5344, 6076
PshAI GACNNNNGTC 2 cut(s) 3506, 5136
PshBI ATTAAT 3 cut(s) 1179, 1890, 5882
PsiI TTATAA 2 cut(s) 1430, 5865
Psp124BI GAGCTC 1 cut(s) 3032
Psp1406I AACGTT 2 cut(s) 3764, 5688
Psp5II RGGWCCY 1 cut(s) 6173
Psp6I CCWGG 7 cut(s) 816, 1411, 1849, 2949, 5527, 5555, 5875
PspFI CCCAGC 1 cut(s) 3707
PspGI CCWGG 7 cut(s) 816, 1411, 1849, 2949, 5527, 5555, 5875
PspOMI GGGCCC 1 cut(s) 4161
PspPI GGNCC 6 cut(s) 1409, 4161, 4162, 5670, 6173, 6592
PspPPI RGGWCCY 1 cut(s) 6173
PstI CTGCAG 1 cut(s) 3447
PstNI CAGNNNCTG 1 cut(s) 3442
PsyI GACNNNGTC 1 cut(s) 2355
PvuI CGATCG 1 cut(s) 6094
PvuII CAGCTG 2 cut(s) 1246, 4008
RsaI GTAC 9 cut(s) 393, 1651, 2373, 3410, 3643, 3746, 3925, 4330, 4561
RsaNI GTAC 9 cut(s) 392, 1650, 2372, 3409, 3642, 3745, 3924, 4329, 4560
RseI CAYNNNNRTG 5 cut(s) 5063, 5343, 5584, 5649, 5781
SacI GAGCTC 1 cut(s) 3032
SapI GCTCTTC 1 cut(s) 1524
Sau96I GGNCC 6 cut(s) 1409, 4161, 4162, 5670, 6173, 6592
ScaI AGTACT 3 cut(s) 393, 2373, 3746
SduI GDGCHC 9 cut(s) 461, 995, 2970, 3032, 4165, 4814, 5556, 6028, 6247
SfcI CTRYAG 6 cut(s) 960, 1928, 3443, 4389, 5224, 6399
Sfr274I CTCGAG 4 cut(s) 599, 4250, 5405, 6260
SfuI TTCGAA 2 cut(s) 197, 846
SinI GGWCC 3 cut(s) 1409, 6173, 6592
SlaI CTCGAG 4 cut(s) 599, 4250, 5405, 6260
SmaI CCCGGG 2 cut(s) 2971, 4035
SmiMI CAYNNNNRTG 5 cut(s) 5063, 5343, 5584, 5649, 5781
SmlI CTYRAG 7 cut(s) 599, 994, 2710, 3413, 4250, 5405, 6260
SmoI CTYRAG 7 cut(s) 599, 994, 2710, 3413, 4250, 5405, 6260
SpeI ACTAGT 1 cut(s) 1352
SphI GCATGC 2 cut(s) 3356, 5062
SsiI CCGC 9 cut(s) 161, 230, 299, 523, 1104, 2029, 2326, 4902, 5100
SspI AATATT 2 cut(s) 1601, 5214
SstI GAGCTC 1 cut(s) 3032
StyI CCWWGG 4 cut(s) 2092, 2991, 3658, 3951
TatI WGTACW 4 cut(s) 391, 2371, 3641, 3744
TauI GCSGC 4 cut(s) 233, 1107, 2031, 2328
TseFI GTSAC 6 cut(s) 1151, 1838, 2062, 3485, 3599, 4451
Tsp45I GTSAC 6 cut(s) 1151, 1838, 2062, 3485, 3599, 4451
TspGWI ACGGA 9 cut(s) 1139, 3314, 3923, 4505, 4964, 5466, 5894, 5981, 6515
TspMI CCCGGG 2 cut(s) 2969, 4033
Tth111I GACNNNGTC 1 cut(s) 2355
Van91I CCANNNNNTGG 1 cut(s) 2401
VneI GTGCAC 1 cut(s) 6243
VpaK11BI GGWCC 3 cut(s) 1409, 6173, 6592
VspI ATTAAT 3 cut(s) 1179, 1890, 5882
XagI CCTNNNNNAGG 3 cut(s) 4460, 5164, 6470
XbaI TCTAGA 6 cut(s) 1333, 2263, 2542, 4432, 4654, 6442
XceI RCATGY 7 cut(s) 991, 1788, 3356, 4030, 5062, 5348, 6080
XcmI CCANNNNNNNNNTGG 3 cut(s) 5045, 5246, 5535
XhoI CTCGAG 4 cut(s) 599, 4250, 5405, 6260
XmaI CCCGGG 2 cut(s) 2969, 4033
XmiI GTMKAC 5 cut(s) 1033, 1264, 3148, 4524, 6534
XmnI GAANNNNTTC 6 cut(s) 945, 1518, 1541, 3426, 4341, 6086
ZrmI AGTACT 3 cut(s) 393, 2373, 3746
Zsp2I ATGCAT 2 cut(s) 580, 1920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.