Rh2DG334200

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000307
Physical Location & Seq
Reverse (-)
42831405 .. 42834867
3463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG334200.1

Sequence Viewer

Length: 3033 bp
ATGAGTATACCTGATGAGAGCATCTCTCATCTTTCTGAGCTTACAGAACTACGCTTGTTTAGATGTAGTAAGCTACAGTCCTTGCCGAATAATCTTCCCTTCAATCTAAAACATGTCCATGCACAAGAATGTCCTATGTTGAAGAATAATGCAGATACTTTGACAATATGGGCATCTGGAAAGGGGTTCTGTTTCATAAATTGCAGACAATCTGACCAGGATGATGGTCAGCCAAATCACCTCCCAGTTCCAGTTCCCAAAGACTGTATTGAACTACTCTTTCCTACATACATTGAGGACCGAGTGTATGGCAAAAAACCATTTGAAATTCGTTTTCCACAGTCATGGAGTACAGGACTGAGTGCCGGAATTCCAAATTCATGGAGTCGTTGGAGAAATGGCCCTTCCGTGACAATCCCACTCTCTGATGGTAACAGTACGTGGATGGGACTTGCTCTTTTTGTTGTTTTTGATATCCTTGAGCAAGACATTTTCAACAAGAGCTGGGAATTGGAGGAGACAGTCTGTGATTTTCACACCGACGTTGGGCATGATACTTCCCTAGTTTTTCAAAACTTCATAGACTTCAGGACTGGGTCATATGGTCTTAGTTGCTATGAACCACGTGGTGGGCAATTTAGTGGGTTGTTCGATAAACCAAGTTCACGACTTCGAGCTTCAGTTTCAACAAAGAGACCAAATTTAAAAGTTAGAGGTTGTGGGATACATCTAATATCAGAGGAATATGCTGCAGAGTTTGTTAAAAGTATAGCAACTCAGACTACTAATTCAACTGAGAGTCATCTTGATTCCAATTTTGATCGACATTGCGAGGACATATTAAATGAGGAAACAACTGGTGTCCTGATAGAACAGGGATCTACTAATTCAACTTTCAATGAAGATAGCTGCAGCAAAGTTAACTCTAAGATCAAACTCAGAGGAGAGCTGTCGATACTCTATGAGGGAAGCAAGGGACGTCAGAAGAGTTTCCACTTTTGCTTTCCTGCTTCAGTAATTTCAACCCTGCCTTGGTTCTTCCATCACCATGCAGGGGATGTAACATTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCGACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTATGCTCTCATGATCATGGAAGTATGGTAATGCACGGATCCCTCAAGATAAAGAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAGGGTATCAGGGCATTGTTCAGAACCATTAATGAAGAAATGGAGGTCCAAGTGTGTGGAAGCCGTTTAGCATTTGAGCATGATTTGGAAGACTTGACCCATTCATTAACTGCTGCTGCCAGCACGTTGGGGCAACATGTCCTCACTCAACTATGTTCCCAGGCCCAACCTGTTGATCGGCGCAATGCAGAGGAAGCAGAAATGCCCTTCAATTGCTGTTCCTGGTTTAGAAGAAGTACTGCACTTGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATCATAGATGCTATCTCCAGCAACAGCAAGGTTTTGGGGAGTCTGACCTTACCCAATTGGTCCACTCCAGATCAGTAACATTACTCCACTCGAAAAGCCTTTTGGAAAACAGGGATAGAAACCATGAAAACGTTGCCGAAGAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGATATAGTTCACCACGGTGGAAGAAATGCCTGAAGTTGTTGCTTCGACAGTCCAAGGTGGCTACACTCAGTCTTTGTGGACACGCAATTTCAGCTTTCAAGAATTTTGATCCTTTCTCCCCATACAATATTATATGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAACTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCGTCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCATATCCCTATGTGTTGCATCAATGAAGAGAAACTCAAGTCGCTGCATCTTGGTAGATTCATTTGGCTAACCTACATTCCCCGTATTTTGCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAGAGGCTTGACAGTAGAGAAGAGTGGTATACGTCTCTTGTACAGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAGTGCTGGACATCTTTCTTTGATAATCTGTCTTTCATCCGTCAACTAGTAGAAGCAGATGATCAAAATATTCAACCAAGGATAAGACATGAACTTCCGATGCTTGAAGGTCATATCAAGGTCTTTGAACCGGATTTAATATATAATGCAATCTCCTCGTCTAATGAAATTCCGGAGTGGTTCGGGCACCGCATTGAGGACCCCTGGGATGCCAGCTGTGGCTGGCAATTCCAGTTACCACCACCTTTGAGTGACACAATAGGATTAGCTCTCTTTGTGTCATATCGTATTAGCAGAAATTATTTGCAGGAAAGTTTTTTGTACCCCTTTATTATAACATTGAAAACTGCGGAGAATGGTTTGTCCTCTCTCCATCGGTATCAAATGAGCAATGAAGAATTTGAGTTCCTAAAGCGCTGTTGTATTGTGCATCGTGAATTTTTCTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGACCTCATAGTTTTTTCTGGTCTGAACTGCTGGACGCCGGACATGGTCTATCCCCGTTTTGTGTATGCGGATGAGGTGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGCCCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1010

Amino Acids

116.24

Weight (kDa)

6.13

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 655 - 792 1.7e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2738
AatII GACGTC 1 cut(s) 982
AccB1I GGYRCC 1 cut(s) 2589
AccB7I CCANNNNNTGG 1 cut(s) 629
AccI GTMKAC 2 cut(s) 7, 2353
AccIII TCCGGA 1 cut(s) 2575
AciI CCGC 4 cut(s) 1606, 2593, 2753, 2969
AclI AACGTT 1 cut(s) 1746
AclWI GGATC 4 cut(s) 886, 1224, 1237, 1925
AcuI CTGAAG 5 cut(s) 571, 663, 996, 1799, 1874
AcvI CACGTG 2 cut(s) 626, 1292
AcyI GRCGYC 2 cut(s) 979, 2936
AdeI CACNNNGTG 1 cut(s) 629
AfaI GTAC 9 cut(s) 352, 439, 1144, 1287, 1561, 1572, 1791, 2366, 2726
AfeI AGCGCT 1 cut(s) 2819
AfiI CCNNNNNNNGG 6 cut(s) 546, 629, 1032, 1054, 2409, 2599
AflIII ACRYGT 4 cut(s) 112, 1282, 1291, 1459
AhlI ACTAGT 1 cut(s) 2449
AjnI CCWGG 4 cut(s) 216, 1482, 1544, 2606
AjuI GAANNNNNNNTTGG 2 cut(s) 1700, 1732
AleI CACNNNNGTG 1 cut(s) 1837
Alw26I GTCTC 4 cut(s) 512, 688, 1799, 2363
AlwI GGATC 4 cut(s) 886, 1224, 1237, 1925
Aor13HI TCCGGA 1 cut(s) 2575
Aor51HI AGCGCT 1 cut(s) 2819
AoxI GGCC 3 cut(s) 400, 1485, 2304
ApeKI GCWGC 8 cut(s) 749, 909, 912, 1436, 1439, 1609, 2224, 2992
AseI ATTAAT 1 cut(s) 1353
Asp700I GAANNNNTTC 1 cut(s) 989
AspLEI GCGC 2 cut(s) 1506, 2820
AspS9I GGNCC 7 cut(s) 298, 401, 1257, 1369, 1486, 1675, 2602
AsuC2I CCSGG 1 cut(s) 2132
AsuHPI GGTGA 3 cut(s) 230, 1037, 1824
AvaII GGWCC 5 cut(s) 298, 1257, 1369, 1675, 2602
BaeGI GKGCMC 1 cut(s) 2592
BamHI GGATCC 1 cut(s) 1229
BanI GGYRCC 1 cut(s) 2589
BarI GAAGNNNNNNTAC 2 cut(s) 1135, 1167
BauI CACGAG 1 cut(s) 2057
BbrPI CACGTG 2 cut(s) 626, 1292
BbsI GAAGAC 2 cut(s) 1419, 2007
BbvI GCAGC 8 cut(s) 736, 896, 924, 1423, 1426, 1596, 2211, 3004
BccI CCATC 6 cut(s) 218, 422, 439, 1050, 1098, 2784
BceAI ACGGC 1 cut(s) 1371
BcgI CGANNNNNNTGC 2 cut(s) 2541, 2575
BciT130I CCWGG 4 cut(s) 218, 1484, 1546, 2608
BciVI GTATCC 1 cut(s) 717
BclI TGATCA 3 cut(s) 1096, 1204, 2464
BcnI CCSGG 1 cut(s) 2132
BcoDI GTCTC 4 cut(s) 512, 688, 1799, 2363
BcuI ACTAGT 1 cut(s) 2449
BfaI CTAG 4 cut(s) 563, 2019, 2025, 2450
BfmI CTRYAG 3 cut(s) 74, 750, 910
BfoI RGCGCY 1 cut(s) 2821
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 9 cut(s) 750, 910, 913, 1437, 1440, 1607, 1610, 2225, 2993
BlsI GCNGC 9 cut(s) 751, 911, 914, 1438, 1441, 1608, 1611, 2226, 2994
BmcAI AGTACT 1 cut(s) 1561
Bme1390I CCNGG 5 cut(s) 218, 1484, 1546, 2132, 2608
Bme18I GGWCC 5 cut(s) 298, 1257, 1369, 1675, 2602
BmgT120I GGNCC 7 cut(s) 298, 401, 1257, 1369, 1486, 1675, 2602
BmiI GGNNCC 4 cut(s) 1231, 1258, 2591, 2604
BmrFI CCNGG 5 cut(s) 218, 1484, 1546, 2132, 2608
BmrI ACTGGG 2 cut(s) 239, 603
BmsI GCATC 8 cut(s) 30, 182, 1613, 2208, 2236, 2493, 2602, 2842
BmuI ACTGGG 2 cut(s) 239, 603
BoxI GACNNNNGTC 1 cut(s) 2115
BpiI GAAGAC 2 cut(s) 1419, 2007
BplI GAGNNNNNCTC 4 cut(s) 8, 40, 10, 42
BpmI CTGGAG 3 cut(s) 1616, 1666, 2092
BpuEI CTTGAG 4 cut(s) 500, 1220, 2173, 2201
BpuMI CCSGG 1 cut(s) 2132
BsaAI YACGTR 3 cut(s) 441, 626, 1292
BsaHI GRCGYC 2 cut(s) 979, 2936
BsaI GGTCTC 1 cut(s) 688
BsaJI CCNNGG 9 cut(s) 1031, 1482, 1835, 1875, 2130, 2160, 2480, 2606, 2607
BsaWI WCCGGW 2 cut(s) 2533, 2575
Bsc4I CCNNNNNNNGG 6 cut(s) 546, 629, 1032, 1054, 2409, 2599
Bse1I ACTGG 7 cut(s) 245, 251, 598, 862, 1979, 2075, 2635
Bse3DI GCAATG 3 cut(s) 826, 1513, 2800
BseAI TCCGGA 1 cut(s) 2575
BseBI CCWGG 4 cut(s) 218, 1484, 1546, 2608
BseDI CCNNGG 9 cut(s) 1031, 1482, 1835, 1875, 2130, 2160, 2480, 2606, 2607
BseGI GGATG 7 cut(s) 226, 450, 1063, 2068, 2439, 2617, 2977
BseLI CCNNNNNNNGG 6 cut(s) 546, 629, 1032, 1054, 2409, 2599
BseMI GCAATG 3 cut(s) 826, 1513, 2800
BseMII CTCAG 8 cut(s) 27, 350, 786, 791, 952, 1275, 1794, 1903
BseNI ACTGG 7 cut(s) 245, 251, 598, 862, 1979, 2075, 2635
BseRI GAGGAG 3 cut(s) 530, 957, 2548
BseSI GKGCMC 1 cut(s) 2592
BseXI GCAGC 8 cut(s) 736, 896, 924, 1423, 1426, 1596, 2211, 3004
BseYI CCCAGC 1 cut(s) 504
BsgI GTGCAG 1 cut(s) 1548
BshFI GGCC 3 cut(s) 402, 1487, 2306
BshNI GGYRCC 1 cut(s) 2589
BsiSI CCGG 5 cut(s) 366, 2132, 2534, 2576, 2939
BslFI GGGAC 3 cut(s) 462, 990, 1270
BslI CCNNNNNNNGG 6 cut(s) 546, 629, 1032, 1054, 2409, 2599
BsmAI GTCTC 4 cut(s) 512, 688, 1799, 2363
BsmBI CGTCTC 1 cut(s) 2363
BsmFI GGGAC 3 cut(s) 462, 990, 1270
BsnI GGCC 3 cut(s) 402, 1487, 2306
Bso31I GGTCTC 1 cut(s) 688
Bsp1286I GDGCHC 1 cut(s) 2592
Bsp13I TCCGGA 1 cut(s) 2575
Bsp1407I TGTACA 3 cut(s) 1142, 1789, 2364
BspACI CCGC 4 cut(s) 1606, 2593, 2753, 2969
BspANI GGCC 3 cut(s) 402, 1487, 2306
BspCNI CTCAG 8 cut(s) 28, 351, 787, 790, 951, 1274, 1795, 1902
BspEI TCCGGA 1 cut(s) 2575
BspHI TCATGA 2 cut(s) 1201, 2107
BspLI GGNNCC 4 cut(s) 1231, 1258, 2591, 2604
BspMAI CTGCAG 2 cut(s) 754, 914
BspPI GGATC 4 cut(s) 886, 1224, 1237, 1925
BspT107I GGYRCC 1 cut(s) 2589
BspTNI GGTCTC 1 cut(s) 688
BsrDI GCAATG 3 cut(s) 826, 1513, 2800
BsrGI TGTACA 3 cut(s) 1142, 1789, 2364
BsrI ACTGG 7 cut(s) 245, 251, 598, 862, 1979, 2075, 2635
BssECI CCNNGG 9 cut(s) 1031, 1482, 1835, 1875, 2130, 2160, 2480, 2606, 2607
BssNAI GTATAC 2 cut(s) 8, 2354
BssNI GRCGYC 2 cut(s) 979, 2936
BssSI CACGAG 1 cut(s) 2057
BssT1I CCWWGG 4 cut(s) 1031, 1875, 2160, 2480
Bst1107I GTATAC 2 cut(s) 8, 2354
Bst2BI CACGAG 1 cut(s) 2057
Bst2UI CCWGG 4 cut(s) 218, 1484, 1546, 2608
Bst6I CTCTTC 5 cut(s) 980, 2202, 2339, 2370, 2976
BstACI GRCGYC 2 cut(s) 979, 2936
BstAUI TGTACA 3 cut(s) 1142, 1789, 2364
BstBAI YACGTR 3 cut(s) 441, 626, 1292
BstC8I GCNNGC 3 cut(s) 1444, 2617, 2627
BstDSI CCRYGG 1 cut(s) 1835
BstF5I GGATG 7 cut(s) 226, 450, 1063, 2068, 2439, 2617, 2977
BstH2I RGCGCY 1 cut(s) 2821
BstHHI GCGC 2 cut(s) 1506, 2820
BstMAI GTCTC 4 cut(s) 512, 688, 1799, 2363
BstMWI GCNNNNNNNGC 2 cut(s) 1517, 1913
BstNI CCWGG 4 cut(s) 218, 1484, 1546, 2608
BstNSI RCATGY 3 cut(s) 116, 1286, 1463
BstPAI GACNNNNGTC 1 cut(s) 2115
BstSCI CCNGG 5 cut(s) 216, 1482, 1544, 2130, 2606
BstSFI CTRYAG 3 cut(s) 74, 750, 910
BstSLI GKGCMC 1 cut(s) 2592
BstV1I GCAGC 8 cut(s) 736, 896, 924, 1423, 1426, 1596, 2211, 3004
BstV2I GAAGAC 2 cut(s) 1419, 2007
BstX2I RGATCY 2 cut(s) 878, 1229
BstXI CCANNNNNNTGG 6 cut(s) 224, 345, 381, 1379, 1450, 2871
BstYI RGATCY 2 cut(s) 878, 1229
BstZ17I GTATAC 2 cut(s) 8, 2354
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 3 cut(s) 402, 1487, 2306
BtgI CCRYGG 1 cut(s) 1835
BtsCI GGATG 7 cut(s) 226, 450, 1063, 2068, 2439, 2617, 2977
BtsIMutI CAGTG 2 cut(s) 1986, 2290
Cac8I GCNNGC 3 cut(s) 1444, 2617, 2627
CciI TCATGA 2 cut(s) 1201, 2107
CfoI GCGC 2 cut(s) 1506, 2820
Cfr13I GGNCC 7 cut(s) 298, 401, 1257, 1369, 1486, 1675, 2602
CseI GACGC 3 cut(s) 2105, 2877, 2944
Csp6I GTAC 9 cut(s) 351, 438, 1143, 1286, 1560, 1571, 1790, 2365, 2725
CspCI CAANNNNNGTGG 2 cut(s) 2631, 2666
CviQI GTAC 9 cut(s) 351, 438, 1143, 1286, 1560, 1571, 1790, 2365, 2725
DraI TTTAAA 1 cut(s) 705
DraIII CACNNNGTG 1 cut(s) 629
Eam1104I CTCTTC 5 cut(s) 980, 2202, 2339, 2370, 2976
EarI CTCTTC 5 cut(s) 980, 2202, 2339, 2370, 2976
Eco130I CCWWGG 4 cut(s) 1031, 1875, 2160, 2480
Eco31I GGTCTC 1 cut(s) 688
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 5 cut(s) 298, 1257, 1369, 1675, 2602
Eco47III AGCGCT 1 cut(s) 2819
Eco57I CTGAAG 5 cut(s) 571, 663, 996, 1799, 1874
Eco72I CACGTG 2 cut(s) 626, 1292
EcoO109I RGGNCCY 2 cut(s) 1257, 2602
EcoRI GAATTC 1 cut(s) 369
EcoRII CCWGG 4 cut(s) 216, 1482, 1544, 2606
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 4 cut(s) 1031, 1875, 2160, 2480
ErhI CCWWGG 4 cut(s) 1031, 1875, 2160, 2480
Esp3I CGTCTC 1 cut(s) 2363
FaqI GGGAC 3 cut(s) 462, 990, 1270
FauNDI CATATG 1 cut(s) 601
FbaI TGATCA 3 cut(s) 1096, 1204, 2464
FblI GTMKAC 2 cut(s) 7, 2353
Fnu4HI GCNGC 9 cut(s) 750, 910, 913, 1437, 1440, 1607, 1610, 2225, 2993
FokI GGATG 7 cut(s) 233, 457, 1070, 2075, 2426, 2624, 2984
Fsp4HI GCNGC 9 cut(s) 750, 910, 913, 1437, 1440, 1607, 1610, 2225, 2993
FspBI CTAG 4 cut(s) 563, 2019, 2025, 2450
GlaI GCGC 2 cut(s) 1505, 2819
GluI GCNGC 9 cut(s) 750, 910, 913, 1437, 1440, 1607, 1610, 2225, 2993
GsaI CCCAGC 1 cut(s) 508
GsuI CTGGAG 3 cut(s) 1616, 1666, 2092
HaeII RGCGCY 1 cut(s) 2821
HaeIII GGCC 3 cut(s) 402, 1487, 2306
HapII CCGG 5 cut(s) 366, 2132, 2534, 2576, 2939
HgaI GACGC 3 cut(s) 2105, 2877, 2944
HhaI GCGC 2 cut(s) 1506, 2820
Hin1I GRCGYC 2 cut(s) 979, 2936
Hin6I GCGC 2 cut(s) 1504, 2818
HinP1I GCGC 2 cut(s) 1504, 2818
HincII GTYRAC 2 cut(s) 922, 2447
HindII GTYRAC 2 cut(s) 922, 2447
HpaI GTTAAC 1 cut(s) 922
HpaII CCGG 5 cut(s) 366, 2132, 2534, 2576, 2939
HphI GGTGA 3 cut(s) 230, 1037, 1824
Hpy166II GTNNAC 9 cut(s) 8, 665, 922, 1143, 1678, 1832, 1901, 2354, 2447
Hpy8I GTNNAC 9 cut(s) 8, 665, 922, 1143, 1678, 1832, 1901, 2354, 2447
Hpy99I CGWCG 2 cut(s) 545, 2871
HpyAV CCTTC 5 cut(s) 109, 414, 1540, 1599, 2504
HpyCH4IV ACGT 8 cut(s) 440, 543, 625, 979, 1291, 1448, 1746, 2356
HpyF10VI GCNNNNNNNGC 2 cut(s) 1517, 1913
HpySE526I ACGT 8 cut(s) 440, 543, 625, 979, 1291, 1448, 1746, 2356
Hsp92I GRCGYC 2 cut(s) 979, 2936
HspAI GCGC 2 cut(s) 1504, 2818
Kpn2I TCCGGA 1 cut(s) 2575
Ksp22I TGATCA 3 cut(s) 1096, 1204, 2464
KspAI GTTAAC 1 cut(s) 922
LmnI GCTCC 1 cut(s) 1591
Lsp1109I GCAGC 8 cut(s) 736, 896, 924, 1423, 1426, 1596, 2211, 3004
LweI GCATC 8 cut(s) 30, 182, 1613, 2208, 2236, 2493, 2602, 2842
MaeI CTAG 4 cut(s) 563, 2019, 2025, 2450
MaeII ACGT 8 cut(s) 440, 543, 625, 979, 1291, 1448, 1746, 2356
MaeIII GTNAC 8 cut(s) 409, 431, 1060, 1070, 1690, 2147, 2637, 2654
MfeI CAATTG 3 cut(s) 1534, 1599, 1670
MflI RGATCY 2 cut(s) 878, 1229
MhlI GDGCHC 1 cut(s) 2592
MlyI GAGTC 5 cut(s) 394, 808, 1664, 1801, 2906
MmeI TCCRAC 2 cut(s) 371, 2144
MroI TCCGGA 1 cut(s) 2575
MroXI GAANNNNTTC 1 cut(s) 989
MseI TTAA 8 cut(s) 704, 762, 842, 921, 1353, 1430, 2540, 2988
MslI CAYNNNNRTG 7 cut(s) 117, 127, 343, 1047, 1206, 1837, 2061
MspA1I CMGCKG 1 cut(s) 2619
MspI CCGG 5 cut(s) 366, 2132, 2534, 2576, 2939
MspR9I CCNGG 5 cut(s) 218, 1484, 1546, 2132, 2608
MunI CAATTG 3 cut(s) 1534, 1599, 1670
MvaI CCWGG 4 cut(s) 218, 1484, 1546, 2608
MwoI GCNNNNNNNGC 2 cut(s) 1517, 1913
NciI CCSGG 1 cut(s) 2132
NdeI CATATG 1 cut(s) 601
NlaIV GGNNCC 4 cut(s) 1231, 1258, 2591, 2604
NmuCI GTSAC 3 cut(s) 409, 2147, 2654
NspI RCATGY 3 cut(s) 116, 1286, 1463
OliI CACNNNNGTG 1 cut(s) 1837
PagI TCATGA 2 cut(s) 1201, 2107
PasI CCCWGGG 1 cut(s) 2607
PciI ACATGT 3 cut(s) 112, 1282, 1459
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 5 cut(s) 809, 1315, 2034, 2179, 2238
PflFI GACNNNGTC 2 cut(s) 595, 2945
PflMI CCANNNNNTGG 1 cut(s) 629
PkrI GCNGC 9 cut(s) 751, 911, 914, 1438, 1441, 1608, 1611, 2226, 2994
PleI GAGTC 5 cut(s) 393, 807, 1663, 1801, 2905
PmaCI CACGTG 2 cut(s) 626, 1292
PmlI CACGTG 2 cut(s) 626, 1292
PpsI GAGTC 5 cut(s) 393, 807, 1663, 1801, 2905
Ppu21I YACGTR 3 cut(s) 441, 626, 1292
PpuMI RGGWCCY 2 cut(s) 1257, 2602
PscI ACATGT 3 cut(s) 112, 1282, 1459
PshAI GACNNNNGTC 1 cut(s) 2115
PshBI ATTAAT 1 cut(s) 1353
PsiI TTATAA 1 cut(s) 2738
Psp1406I AACGTT 1 cut(s) 1746
Psp5II RGGWCCY 2 cut(s) 1257, 2602
Psp6I CCWGG 4 cut(s) 216, 1482, 1544, 2606
PspCI CACGTG 2 cut(s) 626, 1292
PspFI CCCAGC 1 cut(s) 504
PspGI CCWGG 4 cut(s) 216, 1482, 1544, 2606
PspN4I GGNNCC 4 cut(s) 1231, 1258, 2591, 2604
PspPI GGNCC 7 cut(s) 298, 401, 1257, 1369, 1486, 1675, 2602
PspPPI RGGWCCY 2 cut(s) 1257, 2602
PsrI GAACNNNNNNTAC 2 cut(s) 1278, 1310
PstI CTGCAG 2 cut(s) 754, 914
PsuI RGATCY 2 cut(s) 878, 1229
PsyI GACNNNGTC 2 cut(s) 595, 2945
PvuII CAGCTG 1 cut(s) 2619
RsaI GTAC 9 cut(s) 352, 439, 1144, 1287, 1561, 1572, 1791, 2366, 2726
RsaNI GTAC 9 cut(s) 351, 438, 1143, 1286, 1560, 1571, 1790, 2365, 2725
RseI CAYNNNNRTG 7 cut(s) 117, 127, 343, 1047, 1206, 1837, 2061
SaqAI TTAA 8 cut(s) 704, 762, 842, 921, 1353, 1430, 2540, 2988
SatI GCNGC 9 cut(s) 750, 910, 913, 1437, 1440, 1607, 1610, 2225, 2993
Sau96I GGNCC 7 cut(s) 298, 401, 1257, 1369, 1486, 1675, 2602
ScaI AGTACT 1 cut(s) 1561
SchI GAGTC 5 cut(s) 394, 808, 1664, 1801, 2906
ScrFI CCNGG 5 cut(s) 218, 1484, 1546, 2132, 2608
SduI GDGCHC 1 cut(s) 2592
SfaNI GCATC 8 cut(s) 30, 182, 1613, 2208, 2236, 2493, 2602, 2842
SfcI CTRYAG 3 cut(s) 74, 750, 910
SinI GGWCC 5 cut(s) 298, 1257, 1369, 1675, 2602
SmiMI CAYNNNNRTG 7 cut(s) 117, 127, 343, 1047, 1206, 1837, 2061
SmlI CTYRAG 4 cut(s) 479, 1235, 2152, 2216
SmoI CTYRAG 4 cut(s) 479, 1235, 2152, 2216
SpeI ACTAGT 1 cut(s) 2449
SsiI CCGC 4 cut(s) 1606, 2593, 2753, 2969
SspI AATATT 2 cut(s) 1951, 2473
SspMI CTAG 4 cut(s) 563, 2019, 2025, 2450
StyD4I CCNGG 5 cut(s) 216, 1482, 1544, 2130, 2606
StyI CCWWGG 4 cut(s) 1031, 1875, 2160, 2480
TaiI ACGT 8 cut(s) 443, 546, 628, 982, 1294, 1451, 1749, 2359
TaqI TCGA 8 cut(s) 651, 673, 823, 953, 1154, 1706, 1867, 3013
TaqII GACCGA 1 cut(s) 315
TatI WGTACW 6 cut(s) 350, 1142, 1559, 1570, 1789, 2364
TauI GCSGC 1 cut(s) 1609
TfiI GAWTC 5 cut(s) 809, 1315, 2034, 2179, 2238
Tru1I TTAA 8 cut(s) 704, 762, 842, 921, 1353, 1430, 2540, 2988
Tru9I TTAA 8 cut(s) 704, 762, 842, 921, 1353, 1430, 2540, 2988
TscAI CASTG 2 cut(s) 1986, 2290
TseFI GTSAC 3 cut(s) 409, 2147, 2654
TseI GCWGC 8 cut(s) 749, 909, 912, 1436, 1439, 1609, 2224, 2992
Tsp45I GTSAC 3 cut(s) 409, 2147, 2654
TspGWI ACGGA 4 cut(s) 397, 1242, 2291, 2432
TspRI CASTG 2 cut(s) 1986, 2290
Tth111I GACNNNGTC 2 cut(s) 595, 2945
Van91I CCANNNNNTGG 1 cut(s) 629
VpaK11BI GGWCC 5 cut(s) 298, 1257, 1369, 1675, 2602
VspI ATTAAT 1 cut(s) 1353
XceI RCATGY 3 cut(s) 116, 1286, 1463
XmiI GTMKAC 2 cut(s) 7, 2353
XmnI GAANNNNTTC 1 cut(s) 989
XspI CTAG 4 cut(s) 563, 2019, 2025, 2450
ZraI GACGTC 1 cut(s) 980
ZrmI AGTACT 1 cut(s) 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.