Rh2CG192600

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000307
Physical Location & Seq
Reverse (-)
18174592 .. 18182681
8090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG192600.1

Sequence Viewer

Length: 4056 bp
ATGGATGTGCGTATAGAAAAGTTGGAGTCTTACTTGGATCTAAGGTCGGATGACGTTCTTAGCATAGGGATTTGGGGGATGGGGGGCATTGGTAAGACAACTCTGGCAAAAGAAGTTTTCAAAAAGATTCGTAACCAATTTGATAGGAGCGGCTTTCTTTCCAATGTTAGATTGCATTCCGAAGAAAGAGCTCTAGTTGAGTTGCAAAAACTTCTGTGTGGATCCTCTTTCGAGAACAACAACATAAATATAGACACTGTTGGAGAAGGGATCAGGTTGTTAAAGAAAAAATTACTTAAGAAAAAGGTGCTTATTGTTCTAGATGATGTTGATGAATTAAAACAACTGAAAGATTTAGCACCTGGAAGGCAGAATGAACAGTACTCTTGGGGCCGAGGGAGTCGACTCATAATAACAACTAGGGATAGGCGTCCATTGATAGAATGTGAAGTACATAAAATATATGAGGCTGAAAAACTTAGTGATGAGGAAGCTTCTCAGCTCATATGTCAACAAGCCTTCAAGAAAAACTATCCACCAGCTGAGTTTGTAGAGCTGTCCAAGAGTTTTGTAAAATATGCCGGTGGCCTTCCTCTAGCTCATAAAGTTAAGGGTTCACAGTTGTGGGGAAGGGAGGTAGATGAATGGTCAGAGGTATTAGATAGACTGGACGAAGATCTGGATAAAGACATTTTTAGTGTGCTTGAAATAAGTTTTGATGAATTACAGGATACAGATAAGAAAATCTTTTTGGACATCGCATGTTTCTTCAATGGTGAGGATCATGTTCGTGTACAAAAGATATTGAAAAGTTGTGGTTTTTCTCCTCGAATAGGCATAACTAACCTCATTGACAAATCTCTGATCAAAATTGAAAGTGAAAAACTGTGGATGCATGAATTACTACGGTGTTTGGGTTGGCATATTGTTCGCAGAGAATCAAAACCTTTTCCGGGCAAACGTAGCAGATTGTGGCTTGATGATAATGCACACAAGTACAAAGGCAGAAGGCCATGGCACTTTGAGGATGCTCGTAATGTACTCGCGAGTAATACCGGAACATCTGCTGTTGAAGGCTTATTCTTAAGCTTGGGTGAAAAAGAAGAAATGCGTTTGAGCAATGACCCGTTCTTAACTATGACCAAACTAAGATTGCTGAAGATTTATAATGTAAATTTTCTGGGCGGGCACTTTACATATCTCTCTAACGATTTACGTTGTCTGGAATGGCACGAATGTCCTCTAAAGTCTCTGCCATCTGGTTTTACGCCCTATCAGCTGGTTGAATTCAAGATGCCTAACAGCTGCATTGAACGACTGTGGAAGAAAACGCCTTCTATGAGAATGCTGACGCTCATGGATCTTTCCAACTGCCAATATCTAGTCACGACCCCTGATTTCAGTGAGGTCCCAAATCTTGAGAGATTGATCCTTGAAGGTTGTAAAGAGTTATCTGCGGTTCACCCAACAATTAGGGATCTCCAGCATCTGATTTTATTGAATTTGAAAGGCTGTGCAAGTCTAAAGAGCCTTCCCCCATCAATCAGCTTGAGATCTCTTCAAACTTTTATTCTTTCGGGATGTTCAAAACTGGAAGAGTTTCCGGAGATTGTGGGAAACATGCAAACTTTGTCAGAACTTTATTTAGATGGCACCGCTATAAGGGAGGTGCCTGTATCAATCCAGCTTTTAACAGGCCTTGTTTTGCTAAATCTATGTGGCTGCAAGAACCTTCCGAGTCTTCCAAGCGCTCTTTGTAGAAGTTTGACATCACTGAAATTTCTTTATTTGTCATGCTGCTCTAGTTTGGACAAACTGCCAGAAAACATAGGCTCCTTGGAACACTTGGAGGAGCTTGATGCATGTTATACTGCTATAAGAAAAGTGCCTGAGTCCATTTCACTACTCAAGAATCTTAAACTACTGTGTTTCCATGGTTGCTCTGGATACACAGGTCTAGAGATGCCAAACAAGTTCTCAGGTCTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCCCAGAAGGAGCAATCCCCAGTGACATTGGCAATTTGTTCTCACTGCAGAGTCTGGATTTAAGTGAAAACAACTTTTTCACCATTCCTGAAAGCATCTCTCAACTTTCTGAGCTTACAGAAATTTTTCTGTTTAGGTGTAGCAAACTACAATCGTTGCCAAAAGATCTTCCATCAAGACTAAGAACTATAAATTTACGGGACTGTCCTTTGCTGACAGATTCTTCATATAATTCGATGAGATATCCACCTCGAAAGGGTTTAAGTACTATCATAAGTTGTCGAAAACCGGAGGAGGATGAGCAGTTGCCAATATTACTATCTGAACTCCATGAGGTGTCTCTTTCTCTGCAGTTGCCAATGCTACCTGAACTCCATGAGTTTCGTTTGGGAAATCTGGAAAAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAACCTTAAGAAATTGATCCTTGAAGGTTGTGAAAAATTATCAGAGGTTCACCCAACATTTGGGAGTCTCCAACATCTGATTTTTTTGAATATGAAAGGATGTGTAGATCTAGAGAGCCTTCCCCGCTTCATCTGCATGAAATTTCTTGAAATCTGTATTCTTTCAGGATGTTCAAAGCTTAAAGAGTTTCCGGAGATTAATGGAGATATGGATAAATTGTCACAACTGCATTTAGATGGGACGGCTTTAGAGAATCTGCTGATACCAATGCAGCATTTGAAATGCCCTATTGTGATAAATCTAAGAGGTTGCAAGAACCTATTGACTATTCCAATCCTTTTGAGTCTGAAAGCTCTCAATCTGTCAGGCTGCTCTCGTATATCCAGATTTCCAAATAACTGGGGAAGCATGGCACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACACGAGTACCCCAGTCCATTTCATTTATGGAGAAGCTTAAAGTGTTGTCTTTCTGTGGATGTAAAGGTTTGCAGTTGCCTAACCGGTTCTCGCAATTAAGCTTTTTGACATCATTAAATCTACGGGGGTGTGGTCTAGCAGAACTAACAGTCCTTGCTAGCCTCTGTGGCTTATCCTCACTGCAAAAGTTGGATTTGAGTGGAAACAAATTTGTGAGTATACCTAGTGAAATTAGTCGCTTATCCTCTTTGAAGCGATTGAATTTGAGTAGGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGGCTTTCCTCTTTGCAGCAATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGGCTTTCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAATGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCTATTGGATTTGAGTATGAATGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAATGACTTGGTGAGTATACCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGACTCTCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGACTTTCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTGCGAACGACTTGGTGAGTATACCCGATGCAATTGATCGACTCTCCTCGTTGGAGCGATTGGATTTGAGTAGGAACAAGCTGGTGAGCATACCAGATGCAATCGGTCTCTTAACATCCTTGAAGCATTTAGATTGGAGTGAAAACAACTCTGTCAGTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

1351

Amino Acids

150.53

Weight (kDa)

5.79

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 5 - 177 1.3e-19 NB-ARC domain
NACHT PF05729 24 - 171 2.4e-06 NACHT domain
WHD_ROQ1 PF23282 245 - 314 1.1e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 517 - 603 2.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 530 - 600 2.2e-06 Leucine-rich repeat region
LRR_14 PF23598 569 - 670 3.3e-12 Leucine-rich repeat region
LRR_14 PF23598 925 - 1018 1.4e-07 Leucine-rich repeat region
LRR_14 PF23598 1007 - 1115 2.1e-13 Leucine-rich repeat region
LRR_8 PF13855 1038 - 1095 7.2e-12 Leucine rich repeat
LRR_8 PF13855 1077 - 1118 1.5e-07 Leucine rich repeat
LRR_8 PF13855 1087 - 1141 2.6e-08 Leucine rich repeat
LRR_14 PF23598 1101 - 1203 3.5e-10 Leucine-rich repeat region
LRR_8 PF13855 1107 - 1164 2.4e-09 Leucine rich repeat
LRR_8 PF13855 1130 - 1187 2.5e-09 Leucine rich repeat
LRR_8 PF13855 1153 - 1210 3.8e-09 Leucine rich repeat
LRR_14 PF23598 1172 - 1274 4.1e-10 Leucine-rich repeat region
LRR_8 PF13855 1192 - 1233 1.2e-06 Leucine rich repeat
LRR_8 PF13855 1199 - 1256 7.4e-10 Leucine rich repeat
LRR_8 PF13855 1245 - 1302 5.9e-11 Leucine rich repeat
LRR_14 PF23598 1262 - 1344 2.6e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
AccB1I GGYRCC 2 cut(s) 1652, 1669
AccB7I CCANNNNNTGG 1 cut(s) 2543
AccBSI CCGCTC 1 cut(s) 150
AccII CGCG 1 cut(s) 1046
AccIII TCCGGA 2 cut(s) 1603, 2674
AciI CCGC 5 cut(s) 150, 1185, 1457, 1656, 2608
AcsI RAATTY 9 cut(s) 1174, 1286, 1501, 1778, 2145, 2215, 2624, 3143, 3196
AcuI CTGAAG 1 cut(s) 1178
AcyI GRCGYC 1 cut(s) 430
AfeI AGCGCT 1 cut(s) 1750
AfiI CCNNNNNNNGG 5 cut(s) 833, 953, 1662, 2279, 2543
AflII CTTAAG 3 cut(s) 296, 1084, 2489
AgeI ACCGGT 1 cut(s) 3018
AjnI CCWGG 1 cut(s) 361
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AleI CACNNNNGTG 1 cut(s) 622
AloI GAACNNNNNNTCC 2 cut(s) 3069, 3101
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 4 cut(s) 1254, 2367, 2555, 4004
AlwNI CAGNNNCTG 3 cut(s) 1489, 2077, 4053
Aor13HI TCCGGA 2 cut(s) 1603, 2674
Aor51HI AGCGCT 1 cut(s) 1750
AoxI GGCC 4 cut(s) 391, 586, 1010, 1696
ApoI RAATTY 9 cut(s) 1174, 1286, 1501, 1778, 2145, 2215, 2624, 3143, 3196
AseI ATTAAT 1 cut(s) 2682
AsiGI ACCGGT 1 cut(s) 3018
Asp700I GAANNNNTTC 2 cut(s) 1599, 2148
AspA2I CCTAGG 1 cut(s) 2008
AspLEI GCGC 1 cut(s) 1751
AspS9I GGNCC 2 cut(s) 391, 1408
AsuC2I CCSGG 1 cut(s) 954
AsuNHI GCTAGC 1 cut(s) 3092
AvaII GGWCC 1 cut(s) 1408
AvrII CCTAGG 1 cut(s) 2008
BaeGI GKGCMC 1 cut(s) 1191
BaeI ACNNNNGTAYC 4 cut(s) 1939, 1972, 2925, 2958
BamHI GGATCC 1 cut(s) 221
BanI GGYRCC 2 cut(s) 1652, 1669
BanII GRGCYC 1 cut(s) 193
BauI CACGAG 1 cut(s) 2937
BbsI GAAGAC 1 cut(s) 1733
Bbv12I GWGCWC 1 cut(s) 193
BccI CCATC 6 cut(s) 73, 1264, 1546, 1643, 2203, 2714
BceAI ACGGC 1 cut(s) 2742
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 1 cut(s) 363
BciVI GTATCC 2 cut(s) 724, 1940
BclI TGATCA 1 cut(s) 864
BcnI CCSGG 1 cut(s) 954
BcoDI GTCTC 4 cut(s) 1254, 2367, 2555, 4004
BfmI CTRYAG 2 cut(s) 2069, 2372
BfoI RGCGCY 1 cut(s) 1752
BfrI CTTAAG 3 cut(s) 296, 1084, 2489
BfuI GTATCC 2 cut(s) 724, 1940
BglI GCCNNNNNGGC 1 cut(s) 3102
BglII AGATCT 5 cut(s) 676, 1553, 1988, 2188, 2590
BlnI CCTAGG 1 cut(s) 2008
BmcAI AGTACT 2 cut(s) 383, 2290
Bme1390I CCNGG 2 cut(s) 363, 954
Bme18I GGWCC 1 cut(s) 1408
BmgT120I GGNCC 2 cut(s) 391, 1408
BmiI GGNNCC 7 cut(s) 223, 392, 1410, 1654, 1671, 1834, 2481
BmrFI CCNGG 2 cut(s) 363, 954
BmrI ACTGGG 3 cut(s) 2037, 2893, 2941
BmtI GCTAGC 1 cut(s) 3096
BmuI ACTGGG 3 cut(s) 2037, 2893, 2941
BpiI GAAGAC 1 cut(s) 1733
BpmI CTGGAG 1 cut(s) 1466
BpuEI CTTGAG 3 cut(s) 1439, 1570, 1892
BpuMI CCSGG 1 cut(s) 954
BsaHI GRCGYC 1 cut(s) 430
BsaI GGTCTC 1 cut(s) 4004
BsaJI CCNNGG 5 cut(s) 394, 1013, 1836, 1933, 2008
BsaWI WCCGGW 5 cut(s) 1055, 1603, 2311, 2674, 3018
BsaXI ACNNNNNCTCC 4 cut(s) 1817, 1847, 2379, 2409
Bsc4I CCNNNNNNNGG 5 cut(s) 833, 953, 1662, 2279, 2543
Bse118I RCCGGY 2 cut(s) 581, 3018
Bse1I ACTGG 9 cut(s) 672, 1596, 2043, 2888, 2947, 3448, 3517, 3586, 3655
Bse3DI GCAATG 1 cut(s) 1126
BseAI TCCGGA 2 cut(s) 1603, 2674
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 5 cut(s) 394, 1013, 1836, 1933, 2008
BseLI CCNNNNNNNGG 5 cut(s) 833, 953, 1662, 2279, 2543
BseMI GCAATG 1 cut(s) 1126
BseMII CTCAG 5 cut(s) 512, 534, 1881, 1992, 2124
BseNI ACTGG 9 cut(s) 672, 1596, 2043, 2888, 2947, 3448, 3517, 3586, 3655
BseRI GAGGAG 6 cut(s) 816, 1865, 2330, 2921, 3721, 3928
BseSI GKGCMC 1 cut(s) 1191
Bsh1236I CGCG 1 cut(s) 1046
BshFI GGCC 4 cut(s) 393, 588, 1012, 1698
BshNI GGYRCC 2 cut(s) 1652, 1669
BshTI ACCGGT 1 cut(s) 3018
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 7 cut(s) 582, 953, 1056, 1604, 2312, 2675, 3019
BslFI GGGAC 3 cut(s) 1394, 2237, 2737
BslI CCNNNNNNNGG 5 cut(s) 833, 953, 1662, 2279, 2543
BsmAI GTCTC 4 cut(s) 1254, 2367, 2555, 4004
BsmFI GGGAC 3 cut(s) 1394, 2237, 2737
BsmI GAATGC 2 cut(s) 175, 1350
BsnI GGCC 4 cut(s) 393, 588, 1012, 1698
Bso31I GGTCTC 1 cut(s) 4004
Bsp1286I GDGCHC 2 cut(s) 193, 1191
Bsp13I TCCGGA 2 cut(s) 1603, 2674
Bsp1407I TGTACA 1 cut(s) 793
Bsp19I CCATGG 2 cut(s) 1013, 1933
Bsp68I TCGCGA 1 cut(s) 1046
BspACI CCGC 5 cut(s) 150, 1185, 1457, 1656, 2608
BspANI GGCC 4 cut(s) 393, 588, 1012, 1698
BspCNI CTCAG 5 cut(s) 511, 535, 1882, 1991, 2125
BspEI TCCGGA 2 cut(s) 1603, 2674
BspFNI CGCG 1 cut(s) 1046
BspLI GGNNCC 7 cut(s) 223, 392, 1410, 1654, 1671, 1834, 2481
BspMAI CTGCAG 2 cut(s) 2073, 2376
BspOI GCTAGC 1 cut(s) 3096
BspT107I GGYRCC 2 cut(s) 1652, 1669
BspTI CTTAAG 3 cut(s) 296, 1084, 2489
BspTNI GGTCTC 1 cut(s) 4004
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 1126
BsrFI RCCGGY 2 cut(s) 581, 3018
BsrGI TGTACA 1 cut(s) 793
BsrI ACTGG 9 cut(s) 672, 1596, 2043, 2888, 2947, 3448, 3517, 3586, 3655
BssAI RCCGGY 2 cut(s) 581, 3018
BssECI CCNNGG 5 cut(s) 394, 1013, 1836, 1933, 2008
BssNI GRCGYC 1 cut(s) 430
BssSI CACGAG 1 cut(s) 2937
BssT1I CCWWGG 4 cut(s) 1013, 1836, 1933, 2008
Bst2BI CACGAG 1 cut(s) 2937
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 9 cut(s) 259, 381, 621, 888, 909, 1320, 1926, 2228, 3085
Bst6I CTCTTC 2 cut(s) 1563, 1590
BstACI GRCGYC 1 cut(s) 430
BstAFI CTTAAG 3 cut(s) 296, 1084, 2489
BstAUI TGTACA 1 cut(s) 793
BstC8I GCNNGC 2 cut(s) 1187, 3094
BstDSI CCRYGG 2 cut(s) 1013, 1933
BstENI CCTNNNNNAGG 1 cut(s) 831
BstFNI CGCG 1 cut(s) 1046
BstH2I RGCGCY 1 cut(s) 1752
BstHHI GCGC 1 cut(s) 1751
BstMAI GTCTC 4 cut(s) 1254, 2367, 2555, 4004
BstMWI GCNNNNNNNGC 5 cut(s) 963, 1276, 2607, 2616, 3102
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 3 cut(s) 765, 1624, 1866
BstSCI CCNGG 2 cut(s) 361, 952
BstSFI CTRYAG 2 cut(s) 2069, 2372
BstSLI GKGCMC 1 cut(s) 1191
BstUI CGCG 1 cut(s) 1046
BstV2I GAAGAC 1 cut(s) 1733
BstXI CCANNNNNNTGG 1 cut(s) 2883
BsuI GTATCC 2 cut(s) 724, 1940
BsuRI GGCC 4 cut(s) 393, 588, 1012, 1698
BtgI CCRYGG 2 cut(s) 1013, 1933
BtgZI GCGATG 1 cut(s) 742
BtsI GCAGTG 2 cut(s) 2066, 3113
BtsIMutI CAGTG 6 cut(s) 255, 1408, 1772, 2050, 2066, 3113
BtuMI TCGCGA 1 cut(s) 1046
Cac8I GCNNGC 2 cut(s) 1187, 3094
CaiI CAGNNNCTG 3 cut(s) 1489, 2077, 4053
CfoI GCGC 1 cut(s) 1751
Cfr10I RCCGGY 2 cut(s) 581, 3018
Cfr13I GGNCC 2 cut(s) 391, 1408
CseI GACGC 2 cut(s) 419, 1360
CspAI ACCGGT 1 cut(s) 3018
Eam1104I CTCTTC 2 cut(s) 1563, 1590
EarI CTCTTC 2 cut(s) 1563, 1590
Ecl136II GAGCTC 1 cut(s) 191
Eco130I CCWWGG 4 cut(s) 1013, 1836, 1933, 2008
Eco147I AGGCCT 1 cut(s) 1698
Eco24I GRGCYC 1 cut(s) 193
Eco31I GGTCTC 1 cut(s) 4004
Eco32I GATATC 1 cut(s) 2267
Eco47I GGWCC 1 cut(s) 1408
Eco47III AGCGCT 1 cut(s) 1750
Eco53kI GAGCTC 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 1178
EcoICRI GAGCTC 1 cut(s) 191
EcoNI CCTNNNNNAGG 1 cut(s) 831
EcoO109I RGGNCCY 1 cut(s) 1408
EcoRI GAATTC 1 cut(s) 1286
EcoRII CCWGG 1 cut(s) 361
EcoRV GATATC 1 cut(s) 2267
EcoT14I CCWWGG 4 cut(s) 1013, 1836, 1933, 2008
EcoT22I ATGCAT 2 cut(s) 897, 1864
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 4 cut(s) 1013, 1836, 1933, 2008
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FaqI GGGAC 3 cut(s) 1394, 2237, 2737
FauI CCCGC 2 cut(s) 1178, 2615
FauNDI CATATG 1 cut(s) 506
FbaI TGATCA 1 cut(s) 864
FriOI GRGCYC 1 cut(s) 193
GlaI GCGC 1 cut(s) 1750
GsuI CTGGAG 1 cut(s) 1466
HaeII RGCGCY 1 cut(s) 1752
HaeIII GGCC 4 cut(s) 393, 588, 1012, 1698
HapII CCGG 7 cut(s) 582, 953, 1056, 1604, 2312, 2675, 3019
HgaI GACGC 2 cut(s) 419, 1360
HhaI GCGC 1 cut(s) 1751
Hin1I GRCGYC 1 cut(s) 430
Hin6I GCGC 1 cut(s) 1749
HinP1I GCGC 1 cut(s) 1749
HincII GTYRAC 4 cut(s) 404, 512, 3725, 3794
HindII GTYRAC 4 cut(s) 404, 512, 3725, 3794
HindIII AAGCTT 5 cut(s) 492, 1087, 2660, 2969, 3034
HpaII CCGG 7 cut(s) 582, 953, 1056, 1604, 2312, 2675, 3019
HpyCH4III ACNGT 9 cut(s) 259, 381, 621, 888, 909, 1320, 1926, 2228, 3085
HpyCH4IV ACGT 3 cut(s) 54, 961, 1216
HpyF10VI GCNNNNNNNGC 5 cut(s) 963, 1276, 2607, 2616, 3102
HpySE526I ACGT 3 cut(s) 54, 961, 1216
Hsp92I GRCGYC 1 cut(s) 430
HspAI GCGC 1 cut(s) 1749
Kpn2I TCCGGA 2 cut(s) 1603, 2674
Ksp22I TGATCA 1 cut(s) 864
LmnI GCTCC 6 cut(s) 147, 1838, 1852, 2033, 2908, 3946
MaeII ACGT 3 cut(s) 54, 961, 1216
MaeIII GTNAC 4 cut(s) 131, 1384, 2045, 2703
MbiI CCGCTC 1 cut(s) 150
MfeI CAATTG 8 cut(s) 3234, 3303, 3329, 3372, 3717, 3786, 3855, 3924
MhlI GDGCHC 2 cut(s) 193, 1191
MmeI TCCRAC 6 cut(s) 27, 241, 1392, 2578, 3105, 3924
Mph1103I ATGCAT 2 cut(s) 897, 1864
MroI TCCGGA 2 cut(s) 1603, 2674
MroXI GAANNNNTTC 2 cut(s) 1599, 2148
MslI CAYNNNNRTG 4 cut(s) 622, 789, 2618, 2718
MspA1I CMGCKG 3 cut(s) 542, 1279, 1305
MspCI CTTAAG 3 cut(s) 296, 1084, 2489
MspI CCGG 7 cut(s) 582, 953, 1056, 1604, 2312, 2675, 3019
MspR9I CCNGG 2 cut(s) 363, 954
MunI CAATTG 8 cut(s) 3234, 3303, 3329, 3372, 3717, 3786, 3855, 3924
Mva1269I GAATGC 2 cut(s) 175, 1350
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 1046
MwoI GCNNNNNNNGC 5 cut(s) 963, 1276, 2607, 2616, 3102
NciI CCSGG 1 cut(s) 954
NcoI CCATGG 2 cut(s) 1013, 1933
NdeI CATATG 1 cut(s) 506
NheI GCTAGC 1 cut(s) 3092
NlaIV GGNNCC 7 cut(s) 223, 392, 1410, 1654, 1671, 1834, 2481
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 3 cut(s) 1384, 2045, 2703
NruI TCGCGA 1 cut(s) 1046
NsiI ATGCAT 2 cut(s) 897, 1864
NspI RCATGY 3 cut(s) 765, 1624, 1866
OliI CACNNNNGTG 1 cut(s) 622
PceI AGGCCT 1 cut(s) 1698
PctI GAATGC 2 cut(s) 175, 1350
PdmI GAANNNNTTC 2 cut(s) 1599, 2148
PfeI GAWTC 5 cut(s) 127, 938, 1912, 2243, 2737
PflMI CCANNNNNTGG 1 cut(s) 2543
PinAI ACCGGT 1 cut(s) 3018
PpuMI RGGWCCY 1 cut(s) 1408
PshBI ATTAAT 1 cut(s) 2682
PsiI TTATAA 1 cut(s) 1167
Psp124BI GAGCTC 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 1408
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 7 cut(s) 223, 392, 1410, 1654, 1671, 1834, 2481
PspPI GGNCC 2 cut(s) 391, 1408
PspPPI RGGWCCY 1 cut(s) 1408
PstI CTGCAG 2 cut(s) 2073, 2376
PstNI CAGNNNCTG 3 cut(s) 1489, 2077, 4053
PvuII CAGCTG 3 cut(s) 542, 1279, 1305
RruI TCGCGA 1 cut(s) 1046
RseI CAYNNNNRTG 4 cut(s) 622, 789, 2618, 2718
SacI GAGCTC 1 cut(s) 193
SalI GTCGAC 3 cut(s) 402, 3723, 3792
Sau96I GGNCC 2 cut(s) 391, 1408
ScaI AGTACT 2 cut(s) 383, 2290
ScrFI CCNGG 2 cut(s) 363, 954
SduI GDGCHC 2 cut(s) 193, 1191
SfcI CTRYAG 2 cut(s) 2069, 2372
SinI GGWCC 1 cut(s) 1408
SmiMI CAYNNNNRTG 4 cut(s) 622, 789, 2618, 2718
SmlI CTYRAG 6 cut(s) 296, 1084, 1418, 1549, 1907, 2489
SmoI CTYRAG 6 cut(s) 296, 1084, 1418, 1549, 1907, 2489
SseBI AGGCCT 1 cut(s) 1698
SsiI CCGC 5 cut(s) 150, 1185, 1457, 1656, 2608
SspI AATATT 1 cut(s) 2337
SstI GAGCTC 1 cut(s) 193
StuI AGGCCT 1 cut(s) 1698
StyD4I CCNGG 2 cut(s) 361, 952
StyI CCWWGG 4 cut(s) 1013, 1836, 1933, 2008
TaaI ACNGT 9 cut(s) 259, 381, 621, 888, 909, 1320, 1926, 2228, 3085
TaiI ACGT 3 cut(s) 57, 964, 1219
TaqII GACCGA 1 cut(s) 3986
TatI WGTACW 6 cut(s) 381, 451, 793, 996, 1039, 2288
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 5 cut(s) 127, 938, 1912, 2243, 2737
TscAI CASTG 6 cut(s) 262, 1408, 1779, 2050, 2073, 3120
TseFI GTSAC 3 cut(s) 1384, 2045, 2703
Tsp45I GTSAC 3 cut(s) 1384, 2045, 2703
TspRI CASTG 6 cut(s) 262, 1408, 1779, 2050, 2073, 3120
Van91I CCANNNNNTGG 1 cut(s) 2543
Vha464I CTTAAG 3 cut(s) 296, 1084, 2489
VpaK11BI GGWCC 1 cut(s) 1408
VspI ATTAAT 1 cut(s) 2682
XagI CCTNNNNNAGG 1 cut(s) 831
XapI RAATTY 9 cut(s) 1174, 1286, 1501, 1778, 2145, 2215, 2624, 3143, 3196
XbaI TCTAGA 3 cut(s) 319, 1957, 2593
XceI RCATGY 3 cut(s) 765, 1624, 1866
XcmI CCANNNNNNNNNTGG 2 cut(s) 1940, 2959
XmaJI CCTAGG 1 cut(s) 2008
XmnI GAANNNNTTC 2 cut(s) 1599, 2148
ZrmI AGTACT 2 cut(s) 383, 2290
Zsp2I ATGCAT 2 cut(s) 897, 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.