RLG00000017517

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000307
Physical Location & Seq
Reverse (-)
17750974 .. 17759206
8233 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017517

Sequence Viewer

Length: 3606 bp
ATGGAACCACCTTCATCATCTTCCTCCCCCAACCCCCAAACAGTTACTTCATCCTCTAACTGGGAACATGATGTCTTCCTCAGTTTCAGGGGCGAGGACACTCGCAATAGTTTTACAGACCATTTATACTATGCTATGAGCCACAGAGGAATCGACACATTTAGAGATACTGAAAAGCTTCGGAGGGGGAAATCCATTTCACCAGAACTTCTGAAAGCAATCGAAGAGTCCAAGTTTGCGGTTACAGTTCTATCAACCAACTATGCTACTTCAACTTGGTGTCTGGATGAACTTGCCCATATTCTCGATTGCAGGAAAGTAAGGGGACTGGAAGTTCTTCCGGTTTTTTATCATGTAGAACCATCTGAGATAAGGAAGCAAACTGGAAACTACGGCAAGGCGTTTGCTAAGCATGAGACAAATTTCAAGGAAAATATGAGAAAGGTGGACAAGTGGAGGAAAGCTTTAGAGGATATAGCTGGTCTCTCCGGGTGGCATGTGACACAGGATAGCTTTGGGGTAGATATGAGGGAGAAAATTATCAACGCATCAGACGGAGCGAAGGAGGAGAGGAATCTCATATGTCAACAAGCCTTCAAGAAAAACTATCCGCCAGCTGAGTTTGTAGAGCTGTCCAAGAGTTTTGTAAAATATGCCGGTGGCCTTCCTCTAGCTCATAAAGTTAAGGGTTCACAATTGTGGGGAAGGGAGGTAGATGAATGGTCAGAGGTATTAGATAGACTGGACGAAGATCTGGATAAAGACATTTTTAGTGTGCTTGAAATAAGTTTTGATGGATTACAGGATACAGATAAGAAAATCTTTTTGGACATCGCATGTTTCTTCAATGGTGAGGATTATGTTCGTGTACAAAAGATATTGAAAAGTTGTGGTTTTTCTCCTCGAATAGGCATAACTAACCTCATTGACAAATCTCTGATCAAAATTGAAAGTGAAAAACTGTGGATGCATGAATTACTACGGTGTCTGGGTTGGCATATTGTTCGCAGAGAATCAAAACCTTTTCCGGGCAAACGTAGCAGATTGTGGCTTGATGATAATGCACACAAGTACAAAGGCAGAAGGTCATGGCACTTTGAGGATGCTCGTAATGTACTCGCGAGTAATACCGAGAAATTCTTTTATGAGGCTGCTGTATGCAGCCGCCCTCTCACAATGCCTTCCATGAGAATGCTGACACTCATGGATCTTTCCAACTGCAAATATCTAGTCACGACCCCTGACTTCAGTGAGGTCCCAAATCTTGAGAGATTGATCCTTGAAGGTTGTAAAGAGTTATCTGCGGTTCACCCAACAATTAGGGATCTCCAGCATCTGATTTTATTGAATTTGAAAGGCTGTGCAAGTCTAAAGAGCCTTCCCCCATCAATCAGCTTGAGATCTCTTCAAACTTTTATTCTTTCGGGATGTTCAAAACTGGAAGAGTTTCCGGAGATTGTGGGAAACATGCAAACTTTGTCAGAACTTTATTTAGATGGCACCGCTATAAGGGAGGTGCCTGTATCAATCCAGCTTTTAACAGGCCTTGTTTTGCTAAATCTATGTGGCTGCAAGAACCTTCCGAGTCTTCCAAGCGCTCTTTGTAGAAGTTTGACGTCACTGAAATTTCTTTATCTGTCATGGTGCTCTAGTTTGGACAAACTGCCAGAAAACATAGGCTCCTTGGAACACTTGGAGGAGCTTGATGCATGTTATACTGCTATAAGAAAAGTGCCCGAGTCCATTTCACTACTCAAGAATCTTAAACTACTGTGTTTCCATGGCTGCTCTGGATACACAGGTCTAGAGATGCCAAACAAGTTCTCAGGTCTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTTCCAGAAGGAGCAATCCCCAGTGACATTGGCAATTTGTTCTCACTGCAGAGTCTGGATTTAAGTGAAAACAACTTTTTCAACATACCTGAAAGCATCTCTCAACTTTCTGAGCTTACAGAAATTTTTCTGTTTAGGTGTAGCAAACTACAATCGTTGCCAAAAGATCTTCCATCAAGACTAAGAACTATAAATTTATGGGACTGTCCTTTGCTGACAGATTCTTCATATAATTCGATGAGATATCCACCTCGAAAGGGTTTAAGTACTATCATAAGTTGTCGAAAACCGGAGGAGGATGAGCAGTTGCCAATATTACTACCTGAACTCCATGAGGTGTCTCTTTCTCTGCAGTTGCCAATGCTACCTGAACTCCATGAGTTTCGTTTGGGAAATCTGGAAAAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAACCTTAAGAAATTGATCCTTGAAGGATGTTCAAAGCTTAAAGAGTTTCCGGAGATTAAAGGAGATATGGATAAATTGTCACAACTGCATTTAGATGGGACGGCTTTAGAGAATCTGCCAATACCAATGCAGCATTTGAAATGCCCTATTGTGATAAATCTAAGAGGTTGCAAGAACCTATTGACTATTCCAATCCTTTTGAGTCTGAAAGCTCTCAATCTGTCAGGCTGCTCTCGTATATCCAGATTTCCAAATGACTTGGGAAGCATGGCACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACACGAGTACCCCTGTCCATTTCATTTATGGAGAAGCTTAAAGTGCTGTCTTTCTGTGGATGTAAAGGTTTGCAGTTGCCTAATCGGTTCTCGCAATTAAGCTTTTTGACATCATTAAATCTACGGAGGTGTGGTCTAGTAGAACTAACAGTCCTTGCTAGCCTCTGTGGCTTATCCTCATTGGAAAAGTTGGATTTGAGTGGAAACAAATTTGTGAGTATACCTAGTGAAATTAGTCGCTTATCCTCGTTGAAGCGATTGAATTTGAGTAGGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATTCCAGATGCAACTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTATGAATGACTTGTTGAGTATACCAGATGCAACTGGTCGACTCTCCTCTTTGCAGCGATTGGATTTGAGTACGAACGACTTGGTGAGTATACCAGATGCAATTGGTCAACTTTCCTCTTTGCAGCGATTGGATTTGAGTATGAACGACTTGGTGAGTATACCAGATGCAATTGGTGGCTTATCCTCTTTGCAGCGATTGGATTTGAGTGCGAACGACTTGGTGAGTATACCAGATGCAATTGGTCGACTCTCCTCATTGGAGCGATTGGATTTGAGTAGGAACAAGCTGCTTACCTCTGATACAGCAACTGGTAATCGGAGTATTGGAGTAAAATGGTTGAATTTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

1202

Amino Acids

133.76

Weight (kDa)

5.93

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 180 1.6e-50 TIR domain
TIR_2 PF13676 25 - 121 1.6e-14 TIR domain
WHD_ROQ1 PF23282 270 - 339 6.9e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 413 - 519 6.9e-06 Leucine-rich repeat region
LRR_13 PF23286 466 - 534 8.4e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 518 - 619 1.6e-12 Leucine-rich repeat region
LRR_14 PF23598 828 - 947 1.8e-09 Leucine-rich repeat region
LRR_14 PF23598 909 - 1017 6.8e-12 Leucine-rich repeat region
LRR_8 PF13855 940 - 997 5.2e-12 Leucine rich repeat
LRR_14 PF23598 957 - 1039 1.2e-11 Leucine-rich repeat region
LRR_8 PF13855 1001 - 1043 4.7e-08 Leucine rich repeat
LRR_14 PF23598 1027 - 1109 1e-08 Leucine-rich repeat region
LRR_8 PF13855 1047 - 1089 4.9e-07 Leucine rich repeat
LRR_8 PF13855 1078 - 1135 5.3e-10 Leucine rich repeat
LRR_14 PF23598 1097 - 1178 3.7e-11 Leucine-rich repeat region
LRR_8 PF13855 1139 - 1181 9.6e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1619
AccB1I GGYRCC 2 cut(s) 1499, 1516
AccII CGCG 1 cut(s) 1121
AccIII TCCGGA 2 cut(s) 1450, 2380
AciI CCGC 5 cut(s) 239, 611, 1165, 1304, 1503
AclWI GGATC 5 cut(s) 1215, 1270, 1332, 2302, 2341
AcsI RAATTY 9 cut(s) 421, 1136, 1348, 1625, 1992, 2062, 2849, 2902, 3595
AcuI CTGAAG 1 cut(s) 1231
AcyI GRCGYC 1 cut(s) 1616
AfaI GTAC 9 cut(s) 870, 1073, 1116, 2137, 2649, 2980, 3049, 3118, 3325
AfeI AGCGCT 1 cut(s) 1597
AfiI CCNNNNNNNGG 5 cut(s) 60, 908, 1028, 1509, 2126
AflII CTTAAG 1 cut(s) 2336
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AleI CACNNNNGTG 1 cut(s) 697
AloI GAACNNNNNNTCC 4 cut(s) 318, 350, 2775, 2807
Alw21I GWGCWC 1 cut(s) 1649
Alw26I GTCTC 3 cut(s) 410, 488, 2214
AlwI GGATC 5 cut(s) 1215, 1270, 1332, 2302, 2341
AlwNI CAGNNNCTG 3 cut(s) 1336, 1924, 3563
Ama87I CYCGRG 1 cut(s) 1736
Aor13HI TCCGGA 2 cut(s) 1450, 2380
Aor51HI AGCGCT 1 cut(s) 1597
AoxI GGCC 2 cut(s) 661, 1543
ApoI RAATTY 9 cut(s) 421, 1136, 1348, 1625, 1992, 2062, 2849, 2902, 3595
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
Asp700I GAANNNNTTC 4 cut(s) 177, 336, 1446, 1995
AspA2I CCTAGG 1 cut(s) 1855
AspLEI GCGC 1 cut(s) 1598
AspS9I GGNCC 1 cut(s) 1255
AsuC2I CCSGG 2 cut(s) 490, 1029
AsuNHI GCTAGC 1 cut(s) 2798
AvaI CYCGRG 1 cut(s) 1736
AvaII GGWCC 1 cut(s) 1255
AvrII CCTAGG 1 cut(s) 1855
BaeGI GKGCMC 1 cut(s) 1737
BaeI ACNNNNGTAYC 4 cut(s) 1786, 1819, 2631, 2664
BanI GGYRCC 2 cut(s) 1499, 1516
BauI CACGAG 1 cut(s) 2643
BbsI GAAGAC 2 cut(s) 67, 1580
Bbv12I GWGCWC 1 cut(s) 1649
BccI CCATC 6 cut(s) 370, 788, 1393, 1490, 2050, 2420
BceAI ACGGC 2 cut(s) 409, 2448
BcgI CGANNNNNNTGC 2 cut(s) 986, 1020
BciVI GTATCC 2 cut(s) 799, 1787
BclI TGATCA 1 cut(s) 939
BcnI CCSGG 2 cut(s) 490, 1029
BcoDI GTCTC 3 cut(s) 410, 488, 2214
BfaI CTAG 8 cut(s) 671, 1229, 1650, 1805, 1856, 2777, 2799, 2865
BfmI CTRYAG 2 cut(s) 1916, 2219
BfoI RGCGCY 1 cut(s) 1599
BfrI CTTAAG 1 cut(s) 2336
BfuI GTATCC 2 cut(s) 799, 1787
BglI GCCNNNNNGGC 1 cut(s) 2808
BglII AGATCT 4 cut(s) 751, 1400, 1835, 2035
BlnI CCTAGG 1 cut(s) 1855
BlpI GCTNAGC 1 cut(s) 408
BmcAI AGTACT 1 cut(s) 2137
Bme1390I CCNGG 2 cut(s) 490, 1029
Bme18I GGWCC 1 cut(s) 1255
BmeT110I CYCGRG 1 cut(s) 1736
BmgT120I GGNCC 1 cut(s) 1255
BmiI GGNNCC 6 cut(s) 6, 1257, 1501, 1518, 1681, 2328
BmrFI CCNGG 2 cut(s) 490, 1029
BmrI ACTGGG 2 cut(s) 70, 1884
BmtI GCTAGC 1 cut(s) 2802
BmuI ACTGGG 2 cut(s) 70, 1884
BpiI GAAGAC 2 cut(s) 67, 1580
BpmI CTGGAG 1 cut(s) 1313
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 3 cut(s) 1286, 1417, 1739
BpuMI CCSGG 2 cut(s) 490, 1029
BsaHI GRCGYC 1 cut(s) 1616
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 3 cut(s) 1683, 1780, 1855
BsaWI WCCGGW 4 cut(s) 340, 1450, 2158, 2380
BsaXI ACNNNNNCTCC 8 cut(s) 1664, 1694, 2181, 2211, 2226, 2256, 2758, 2788
Bsc4I CCNNNNNNNGG 5 cut(s) 60, 908, 1028, 1509, 2126
Bse118I RCCGGY 1 cut(s) 656
Bse1I ACTGG 9 cut(s) 65, 333, 388, 747, 1443, 1890, 3223, 3292, 3568
BseAI TCCGGA 2 cut(s) 1450, 2380
BseDI CCNNGG 3 cut(s) 1683, 1780, 1855
BseGI GGATG 9 cut(s) 50, 292, 972, 1108, 1433, 1868, 2173, 2363, 2705
BseLI CCNNNNNNNGG 5 cut(s) 60, 908, 1028, 1509, 2126
BseMII CTCAG 5 cut(s) 94, 357, 609, 1839, 1971
BseNI ACTGG 9 cut(s) 65, 333, 388, 747, 1443, 1890, 3223, 3292, 3568
BseRI GAGGAG 7 cut(s) 581, 891, 1712, 2177, 2627, 3289, 3496
BseSI GKGCMC 1 cut(s) 1737
Bsh1236I CGCG 1 cut(s) 1121
BshFI GGCC 2 cut(s) 663, 1545
BshNI GGYRCC 2 cut(s) 1499, 1516
BsiHKAI GWGCWC 1 cut(s) 1649
BsiHKCI CYCGRG 1 cut(s) 1736
BsiSI CCGG 7 cut(s) 341, 489, 657, 1028, 1451, 2159, 2381
BslFI GGGAC 4 cut(s) 339, 1241, 2084, 2443
BslI CCNNNNNNNGG 5 cut(s) 60, 908, 1028, 1509, 2126
BsmAI GTCTC 3 cut(s) 410, 488, 2214
BsmFI GGGAC 4 cut(s) 339, 1241, 2084, 2443
BsmI GAATGC 1 cut(s) 1197
BsnI GGCC 2 cut(s) 663, 1545
Bso31I GGTCTC 1 cut(s) 488
BsoBI CYCGRG 1 cut(s) 1736
Bsp1286I GDGCHC 2 cut(s) 1649, 1737
Bsp13I TCCGGA 2 cut(s) 1450, 2380
Bsp1407I TGTACA 1 cut(s) 868
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 1780
Bsp68I TCGCGA 1 cut(s) 1121
BspACI CCGC 5 cut(s) 239, 611, 1165, 1304, 1503
BspANI GGCC 2 cut(s) 663, 1545
BspCNI CTCAG 5 cut(s) 93, 358, 610, 1838, 1972
BspEI TCCGGA 2 cut(s) 1450, 2380
BspFNI CGCG 1 cut(s) 1121
BspLI GGNNCC 6 cut(s) 6, 1257, 1501, 1518, 1681, 2328
BspMAI CTGCAG 2 cut(s) 1920, 2223
BspOI GCTAGC 1 cut(s) 2802
BspPI GGATC 5 cut(s) 1215, 1270, 1332, 2302, 2341
BspT107I GGYRCC 2 cut(s) 1499, 1516
BspTI CTTAAG 1 cut(s) 2336
BspTNI GGTCTC 1 cut(s) 488
BsrFI RCCGGY 1 cut(s) 656
BsrGI TGTACA 1 cut(s) 868
BsrI ACTGG 9 cut(s) 65, 333, 388, 747, 1443, 1890, 3223, 3292, 3568
BssAI RCCGGY 1 cut(s) 656
BssECI CCNNGG 3 cut(s) 1683, 1780, 1855
BssNAI GTATAC 9 cut(s) 2861, 2930, 2999, 3068, 3137, 3275, 3344, 3413, 3482
BssNI GRCGYC 1 cut(s) 1616
BssSI CACGAG 1 cut(s) 2643
BssT1I CCWWGG 3 cut(s) 1683, 1780, 1855
Bst1107I GTATAC 9 cut(s) 2861, 2930, 2999, 3068, 3137, 3275, 3344, 3413, 3482
Bst2BI CACGAG 1 cut(s) 2643
Bst4CI ACNGT 7 cut(s) 43, 247, 963, 984, 1773, 2075, 2791
Bst6I CTCTTC 3 cut(s) 219, 1410, 1437
BstACI GRCGYC 1 cut(s) 1616
BstAFI CTTAAG 1 cut(s) 2336
BstAUI TGTACA 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 615, 2800
BstDEI CTNAG 9 cut(s) 80, 366, 408, 618, 1825, 1832, 1980, 2051, 2492
BstDSI CCRYGG 1 cut(s) 1780
BstENI CCTNNNNNAGG 1 cut(s) 906
BstF5I GGATG 9 cut(s) 50, 292, 972, 1108, 1433, 1868, 2173, 2363, 2705
BstFNI CGCG 1 cut(s) 1121
BstH2I RGCGCY 1 cut(s) 1599
BstHHI GCGC 1 cut(s) 1598
BstMAI GTCTC 3 cut(s) 410, 488, 2214
BstMWI GCNNNNNNNGC 3 cut(s) 1038, 2683, 2808
BstNSI RCATGY 4 cut(s) 500, 840, 1471, 1713
BstSCI CCNGG 2 cut(s) 488, 1027
BstSFI CTRYAG 2 cut(s) 1916, 2219
BstSLI GKGCMC 1 cut(s) 1737
BstUI CGCG 1 cut(s) 1121
BstV2I GAAGAC 2 cut(s) 67, 1580
BstX2I RGATCY 7 cut(s) 751, 1207, 1324, 1400, 1835, 2035, 2307
BstYI RGATCY 7 cut(s) 751, 1207, 1324, 1400, 1835, 2035, 2307
BstZ17I GTATAC 9 cut(s) 2861, 2930, 2999, 3068, 3137, 3275, 3344, 3413, 3482
BsuI GTATCC 2 cut(s) 799, 1787
BsuRI GGCC 2 cut(s) 663, 1545
BtgI CCRYGG 1 cut(s) 1780
BtgZI GCGATG 1 cut(s) 817
BtsCI GGATG 9 cut(s) 50, 292, 972, 1108, 1433, 1868, 2173, 2363, 2705
BtsI GCAGTG 1 cut(s) 1913
BtsIMutI CAGTG 4 cut(s) 1255, 1619, 1897, 1913
BtuMI TCGCGA 1 cut(s) 1121
Cac8I GCNNGC 2 cut(s) 615, 2800
CaiI CAGNNNCTG 3 cut(s) 1336, 1924, 3563
CfoI GCGC 1 cut(s) 1598
Cfr10I RCCGGY 1 cut(s) 656
Cfr13I GGNCC 1 cut(s) 1255
Csp6I GTAC 9 cut(s) 869, 1072, 1115, 2136, 2648, 2979, 3048, 3117, 3324
CviQI GTAC 9 cut(s) 869, 1072, 1115, 2136, 2648, 2979, 3048, 3117, 3324
DdeI CTNAG 9 cut(s) 80, 366, 408, 618, 1825, 1832, 1980, 2051, 2492
Eam1104I CTCTTC 3 cut(s) 219, 1410, 1437
EarI CTCTTC 3 cut(s) 219, 1410, 1437
EciI GGCGGA 1 cut(s) 600
Eco130I CCWWGG 3 cut(s) 1683, 1780, 1855
Eco147I AGGCCT 1 cut(s) 1545
Eco31I GGTCTC 1 cut(s) 488
Eco32I GATATC 1 cut(s) 2114
Eco47I GGWCC 1 cut(s) 1255
Eco47III AGCGCT 1 cut(s) 1597
Eco57I CTGAAG 1 cut(s) 1231
Eco88I CYCGRG 1 cut(s) 1736
EcoNI CCTNNNNNAGG 1 cut(s) 906
EcoO109I RGGNCCY 1 cut(s) 1255
EcoRV GATATC 1 cut(s) 2114
EcoT14I CCWWGG 3 cut(s) 1683, 1780, 1855
EcoT22I ATGCAT 2 cut(s) 972, 1711
ErhI CCWWGG 3 cut(s) 1683, 1780, 1855
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FaqI GGGAC 4 cut(s) 339, 1241, 2084, 2443
FauNDI CATATG 1 cut(s) 581
FbaI TGATCA 1 cut(s) 939
FokI GGATG 9 cut(s) 37, 299, 979, 1115, 1440, 1875, 2180, 2370, 2712
FspBI CTAG 8 cut(s) 671, 1229, 1650, 1805, 1856, 2777, 2799, 2865
GlaI GCGC 1 cut(s) 1597
GsuI CTGGAG 1 cut(s) 1313
HaeII RGCGCY 1 cut(s) 1599
HaeIII GGCC 2 cut(s) 663, 1545
HapII CCGG 7 cut(s) 341, 489, 657, 1028, 1451, 2159, 2381
HhaI GCGC 1 cut(s) 1598
Hin1I GRCGYC 1 cut(s) 1616
Hin6I GCGC 1 cut(s) 1596
HinP1I GCGC 1 cut(s) 1596
HincII GTYRAC 4 cut(s) 587, 3293, 3362, 3500
HindII GTYRAC 4 cut(s) 587, 3293, 3362, 3500
HindIII AAGCTT 5 cut(s) 176, 462, 2366, 2675, 2740
HpaII CCGG 7 cut(s) 341, 489, 657, 1028, 1451, 2159, 2381
HpyCH4III ACNGT 7 cut(s) 43, 247, 963, 984, 1773, 2075, 2791
HpyCH4IV ACGT 2 cut(s) 1036, 1616
HpyF10VI GCNNNNNNNGC 3 cut(s) 1038, 2683, 2808
HpyF3I CTNAG 9 cut(s) 80, 366, 408, 618, 1825, 1832, 1980, 2051, 2492
HpySE526I ACGT 2 cut(s) 1036, 1616
Hsp92I GRCGYC 1 cut(s) 1616
HspAI GCGC 1 cut(s) 1596
Kpn2I TCCGGA 2 cut(s) 1450, 2380
Ksp22I TGATCA 1 cut(s) 939
LmnI GCTCC 6 cut(s) 557, 1685, 1699, 1880, 2614, 3514
MaeI CTAG 8 cut(s) 671, 1229, 1650, 1805, 1856, 2777, 2799, 2865
MaeII ACGT 2 cut(s) 1036, 1616
MaeIII GTNAC 7 cut(s) 43, 241, 499, 1231, 1617, 1892, 2409
MfeI CAATTG 8 cut(s) 695, 2940, 3009, 3078, 3147, 3354, 3423, 3492
MflI RGATCY 7 cut(s) 751, 1207, 1324, 1400, 1835, 2035, 2307
MhlI GDGCHC 2 cut(s) 1649, 1737
MlyI GAGTC 7 cut(s) 236, 1594, 1748, 1930, 2542, 3288, 3495
MmeI TCCRAC 2 cut(s) 1239, 2811
Mph1103I ATGCAT 2 cut(s) 972, 1711
MroI TCCGGA 2 cut(s) 1450, 2380
MroXI GAANNNNTTC 4 cut(s) 177, 336, 1446, 1995
MslI CAYNNNNRTG 3 cut(s) 697, 1190, 2424
MspA1I CMGCKG 1 cut(s) 617
MspCI CTTAAG 1 cut(s) 2336
MspI CCGG 7 cut(s) 341, 489, 657, 1028, 1451, 2159, 2381
MspR9I CCNGG 2 cut(s) 490, 1029
MunI CAATTG 8 cut(s) 695, 2940, 3009, 3078, 3147, 3354, 3423, 3492
Mva1269I GAATGC 1 cut(s) 1197
MvnI CGCG 1 cut(s) 1121
MwoI GCNNNNNNNGC 3 cut(s) 1038, 2683, 2808
NciI CCSGG 2 cut(s) 490, 1029
NcoI CCATGG 1 cut(s) 1780
NdeI CATATG 1 cut(s) 581
NheI GCTAGC 1 cut(s) 2798
NlaIV GGNNCC 6 cut(s) 6, 1257, 1501, 1518, 1681, 2328
NmuCI GTSAC 5 cut(s) 499, 1231, 1617, 1892, 2409
NruI TCGCGA 1 cut(s) 1121
NsiI ATGCAT 2 cut(s) 972, 1711
NspI RCATGY 4 cut(s) 500, 840, 1471, 1713
OliI CACNNNNGTG 1 cut(s) 697
PceI AGGCCT 1 cut(s) 1545
PctI GAATGC 1 cut(s) 1197
PdmI GAANNNNTTC 4 cut(s) 177, 336, 1446, 1995
PfeI GAWTC 6 cut(s) 150, 574, 1013, 1759, 2090, 2443
PleI GAGTC 7 cut(s) 235, 1593, 1747, 1929, 2541, 3288, 3495
PpsI GAGTC 7 cut(s) 235, 1593, 1747, 1929, 2541, 3288, 3495
PpuMI RGGWCCY 1 cut(s) 1255
Psp5II RGGWCCY 1 cut(s) 1255
PspN4I GGNNCC 6 cut(s) 6, 1257, 1501, 1518, 1681, 2328
PspPI GGNCC 1 cut(s) 1255
PspPPI RGGWCCY 1 cut(s) 1255
PstI CTGCAG 2 cut(s) 1920, 2223
PstNI CAGNNNCTG 3 cut(s) 1336, 1924, 3563
PsuI RGATCY 7 cut(s) 751, 1207, 1324, 1400, 1835, 2035, 2307
PvuII CAGCTG 1 cut(s) 617
RruI TCGCGA 1 cut(s) 1121
RsaI GTAC 9 cut(s) 870, 1073, 1116, 2137, 2649, 2980, 3049, 3118, 3325
RsaNI GTAC 9 cut(s) 869, 1072, 1115, 2136, 2648, 2979, 3048, 3117, 3324
RseI CAYNNNNRTG 3 cut(s) 697, 1190, 2424
SalI GTCGAC 2 cut(s) 3291, 3498
Sau96I GGNCC 1 cut(s) 1255
ScaI AGTACT 1 cut(s) 2137
SchI GAGTC 7 cut(s) 236, 1594, 1748, 1930, 2542, 3288, 3495
ScrFI CCNGG 2 cut(s) 490, 1029
SduI GDGCHC 2 cut(s) 1649, 1737
SfcI CTRYAG 2 cut(s) 1916, 2219
SinI GGWCC 1 cut(s) 1255
SmiMI CAYNNNNRTG 3 cut(s) 697, 1190, 2424
SmlI CTYRAG 4 cut(s) 1265, 1396, 1754, 2336
SmoI CTYRAG 4 cut(s) 1265, 1396, 1754, 2336
SseBI AGGCCT 1 cut(s) 1545
SsiI CCGC 5 cut(s) 239, 611, 1165, 1304, 1503
SspI AATATT 1 cut(s) 2184
SspMI CTAG 8 cut(s) 671, 1229, 1650, 1805, 1856, 2777, 2799, 2865
StuI AGGCCT 1 cut(s) 1545
StyD4I CCNGG 2 cut(s) 488, 1027
StyI CCWWGG 3 cut(s) 1683, 1780, 1855
TaaI ACNGT 7 cut(s) 43, 247, 963, 984, 1773, 2075, 2791
TaiI ACGT 2 cut(s) 1039, 1619
TatI WGTACW 4 cut(s) 868, 1071, 1114, 2135
TauI GCSGC 1 cut(s) 1167
TfiI GAWTC 6 cut(s) 150, 574, 1013, 1759, 2090, 2443
TscAI CASTG 4 cut(s) 1255, 1626, 1897, 1920
TseFI GTSAC 5 cut(s) 499, 1231, 1617, 1892, 2409
Tsp45I GTSAC 5 cut(s) 499, 1231, 1617, 1892, 2409
TspDTI ATGAA 9 cut(s) 39, 303, 732, 987, 2085, 2652, 3203, 3272, 3410
TspGWI ACGGA 2 cut(s) 570, 2779
TspRI CASTG 4 cut(s) 1255, 1626, 1897, 1920
Vha464I CTTAAG 1 cut(s) 2336
VpaK11BI GGWCC 1 cut(s) 1255
XagI CCTNNNNNAGG 1 cut(s) 906
XapI RAATTY 9 cut(s) 421, 1136, 1348, 1625, 1992, 2062, 2849, 2902, 3595
XbaI TCTAGA 1 cut(s) 1804
XceI RCATGY 4 cut(s) 500, 840, 1471, 1713
XcmI CCANNNNNNNNNTGG 2 cut(s) 1787, 2665
XmaJI CCTAGG 1 cut(s) 1855
XmnI GAANNNNTTC 4 cut(s) 177, 336, 1446, 1995
XspI CTAG 8 cut(s) 671, 1229, 1650, 1805, 1856, 2777, 2799, 2865
ZraI GACGTC 1 cut(s) 1617
ZrmI AGTACT 1 cut(s) 2137
Zsp2I ATGCAT 2 cut(s) 972, 1711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.