Rh6BG011200

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000307
Physical Location & Seq
Reverse (-)
1711676 .. 1719654
7979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG011200.1

Sequence Viewer

Length: 2775 bp
ATGCATGATTTGCTACAGCAAATGGGTTGGCACATTGTTCGGCAAGAATCTCCTGAGCCGGGAAAACGAAGCAGGTTGTGGCTTAATGAGAATTCCCATGAGTATCAACACAGCCGCCCGTGGTTTGATGAAGATACTCGTGATGTAGTCACCGAGAATCTGGGAACATATGCTGTCCAAGGGGTATATTTGAGCGTGCTGGAAAAAGAAAAGATACAATTGAAAGCTGACCCCTTCTTAAATATGCGCAAGCTAAGATTGTTGAAGATTTGTAATGTCAACTTGGTGGATGTGCGATTTGAATATCTGTCTAACAAGTTACAACTTTTGGAATGGCATGAATGTCCTCTAGAATCTCTGCCATCCAGTTTTCGACCAGATAAACTCGTCGAATTGAAACTGCCCAACAGCCGCATTGAAACACTGTGGAATCAAAGACTCTGTCTGGAGAAGCTAATGCTAATGGATCTTTCGTACTGCCAATACTTGGTCAAGACCCCGGACTTCAGTAATTTCCCAAATCTTGAAGTGTTGACTTTTGCAGGTTGTAGAAAATTATCAGATGTTCACCCAACACTTTGGAGACTCAAAAATTTGAACGCTGCAATTTTTGAAGATTGCACGAGTCTAGAGAGGCTTCCCAAGTCCATCAGCTTGCAATCTCTTGCAATTCTTGTTTTGTCGGGATGTTCAAAACTAGATGAGTTTCCAGAGATAGTGGGAAACAGGGAAACTCTGGTAGCACTCTATTTAGATGGAACAGCTATAAGAGAGCTGCCGACAACAATCGGGTGCTTGAGTGGCCTAATGACGTTAAGTCTATCTGCCTGCAAGAACCTTGTCGGTCTTCCAAGCTTCATTTGCAGTTTAAAGTCTCTGAGTTATCTGCATCTATCACATTCCTCACTCATTGACCAACTTCCAGAAGACATAGGGAGCTTGGAGAATTTGAAGAAGCTTGATGCTTCTCAGACTGCTATAAAAAAAGTGCCTTCGTCCATTGTGCGTCTGAAGCATCTGAAATCATTAGATTTACAGGGATGTAGGCAGGTGCCCGGGGAATGCTCTGATGAATCCTTGGGTTTGCAATTGCCTAAGTCGTTCTCCGGTTTGAGCTCATTGGAAGCACTATATTTAGGTCAATGCGATCTTGCTGAAGGAGCGATTCCTGATGACATTGGCTGCTTATCCTCACTGAGACATTTAAGGTTGGATGAAAACAACTTTGTGAGTATACCTGAAAGCATCTCTCAACTTCCTAACCTTAGAGAAATTCACATCCCAAATTGTAGCAAGCTTCAGTCTTTGCCAAAACATCTTCGAAGGGATGTTAATGTAGAGGCTAGTGGATGTTCCCTGCTGATCAACTATCCAAACCAGCTTAACATATGGCTTTCTGATGATGGGGTCAGTCACATAAATTGTGGAAATTCAAAGCAAGCAAGCTATCCACTTCCAATTCCGGAAGAACAAATTGAAAAATACTTGCCCATGTACCTTGAGGATGAAATTGTTCTTCAGCAGCACCTGCAGGTTCGTTTCGTTGATACAGAAATCCCTGATTGGTGTCGTCATAAGAGTAGTGGCTGGTCTTCTTTAGCCATGCAAGTGTCTCATTCTGAAATTATTAAGAGCTTAGGAGTAGCCCTGTTTGTTGTTTTTGAAATCTTTGAGAGTGGTTACAATTTTGATAAGAGTTGGGAGTTGCAGAAGACCTTTTGTACTTTTAAAGATGACCGAGGACTTGAACATATCTATATCTTTAGAGAATTTGAAAAATTCCTGGCTGGGACATTTGTATTATTTTGCTATGAGCCACTAAAGTACTTTCAAGTTGCTGGACTGTTGACTACAGCAAGTCGATTTGAGGCTTCGGTTTCAACAGAGAGACCAGATCTGCAGGTTAAGTCTTGTGGGATGCAACTAATCTTTCAGGAAGAAGCTACAGAGTTTGCTAAAAAATTAAGTGAGGCTTGTAATCCTCAGACGGATTTCAAATTTGGCAGACATTGTAAGGAATTGATAAACGCCAAGGGTAAGGAAAGCTCCGGTAATATAATGGCACAAAATGAAGCAGAAAAGGTCAGCTGGAAATTTGATTTTAACATGCACCCGGGAGAAGATCTACAGTTGTTGCTTTCAAAAGTCTATGAGGAGGATTTCAATCGGGACATCATATATAATTCAACTTACCCTCATTGCGCTAAACTTTTGGAGTGGTTCGGTGATCATCATCTCAGTAGGGGCCCTTCAGTGAAAATCCCGTTACCACCATCTCTATCTACTGATGGGAATTGGATAGGATTGGCTCTATGTGCACGTTTTTCAGACCTCGAGAATCAAACTACCTCCATAGACAAAGTGAATCAAGAAATTGCTGAGCTTGTTTGTCATTTGGAAACTGAGAACAGTGGTTTCGAACATCTTCACCACTATCGACCAACCAATGAAGAACTCAAGCGGTTATGTCCTACAGAATTCATTTGGCTATCTTATATACCACGTCAGGGGTATCTAGATCAGTTAAATGAGTGTGACCTCTTGGAGGCTTCATTTGCTAGCGAAAGCCGAGGATTGTTTGCTGAAGTGTGTGTTCTCCGTCTTGTCTATCAGCATGATGAGAAAGAGTTTAAGGATCTGTGTATCTTCAGGAACTCGTTGCTGGACAAAAAGGGTAAACAAGTTCTAGAATACCCTGAACCTGAGATTGAATGGGGATCTAATGCAATGTGGAATCTGGAGATCTACGATGACCCAAAATCGAGACATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

924

Amino Acids

105.83

Weight (kDa)

5.38

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 165 - 277 1.9e-07 Leucine-rich repeat region
LRR_13 PF23286 219 - 292 1.5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 249 - 300 4.3e-06 Leucine-rich repeat region
LRR_14 PF23598 286 - 353 3e-13 Leucine-rich repeat region
LRR_13 PF23286 303 - 441 6.6e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1039, 1536
Acc16I TGCGCA 1 cut(s) 248
Acc36I ACCTGC 6 cut(s) 63, 533, 1039, 1522, 1536, 1891
AccB1I GGYRCC 1 cut(s) 1051
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 1 cut(s) 1234
AccIII TCCGGA 1 cut(s) 1462
AciI CCGC 3 cut(s) 115, 412, 2461
AclWI GGATC 3 cut(s) 474, 2643, 2725
AcuI CTGAAG 8 cut(s) 490, 1031, 1176, 1283, 1502, 2235, 2604, 2632
AfaI GTAC 4 cut(s) 476, 1496, 1723, 1826
AfiI CCNNNNNNNGG 4 cut(s) 59, 487, 2507, 2545
AhdI GACNNNNNGTC 1 cut(s) 816
AjiI CACGTC 1 cut(s) 2504
AjnI CCWGG 1 cut(s) 1782
Alw21I GWGCWC 2 cut(s) 1118, 2320
Alw26I GTCTC 6 cut(s) 577, 879, 1192, 1617, 1882, 2758
Alw44I GTGCAC 1 cut(s) 2316
AlwI GGATC 3 cut(s) 474, 2643, 2725
AlwNI CAGNNNCTG 1 cut(s) 1528
Ama87I CYCGRG 3 cut(s) 1055, 2113, 2333
Aor13HI TCCGGA 1 cut(s) 1462
AoxI GGCC 2 cut(s) 802, 2245
ApaI GGGCCC 1 cut(s) 2249
ApaLI GTGCAC 1 cut(s) 2316
ApeKI GCWGC 4 cut(s) 602, 775, 1182, 1522
ArsI GACNNNNNNTTYG 2 cut(s) 427, 459
Asp700I GAANNNNTTC 2 cut(s) 1512, 2424
AspLEI GCGC 2 cut(s) 249, 2204
AspS9I GGNCC 2 cut(s) 2245, 2246
AsuC2I CCSGG 6 cut(s) 60, 500, 1056, 1057, 2114, 2115
AsuHPI GGTGA 4 cut(s) 142, 560, 2237, 2420
AsuII TTCGAA 2 cut(s) 1321, 2418
AsuNHI GCTAGC 1 cut(s) 2558
AvaI CYCGRG 3 cut(s) 1055, 2113, 2333
BaeGI GKGCMC 3 cut(s) 1056, 2249, 2320
BanI GGYRCC 1 cut(s) 1051
BanII GRGCYC 2 cut(s) 1118, 2249
BarI GAAGNNNNNNTAC 2 cut(s) 2233, 2265
BauI CACGAG 2 cut(s) 138, 622
BbsI GAAGAC 4 cut(s) 839, 933, 1584, 1718
Bbv12I GWGCWC 2 cut(s) 1118, 2320
BbvI GCAGC 4 cut(s) 589, 762, 1169, 1534
BccI CCATC 6 cut(s) 370, 656, 749, 1397, 2281, 2282
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 1 cut(s) 1784
BclI TGATCA 2 cut(s) 1362, 2227
BcnI CCSGG 6 cut(s) 60, 500, 1056, 1057, 2114, 2115
BcoDI GTCTC 6 cut(s) 577, 879, 1192, 1617, 1882, 2758
BfaI CTAG 7 cut(s) 350, 629, 698, 1344, 2516, 2559, 2687
BfmI CTRYAG 7 cut(s) 14, 1529, 1851, 1898, 1944, 2126, 2472
BfuAI ACCTGC 6 cut(s) 63, 533, 1039, 1522, 1536, 1891
BglII AGATCT 3 cut(s) 1894, 2122, 2742
BisI GCNGC 6 cut(s) 115, 412, 603, 776, 1183, 1523
BlpI GCTNAGC 1 cut(s) 2379
BlsI GCNGC 6 cut(s) 116, 413, 604, 777, 1184, 1524
BmcAI AGTACT 1 cut(s) 1826
Bme1390I CCNGG 7 cut(s) 60, 500, 1056, 1057, 1784, 2114, 2115
BmeRI GACNNNNNGTC 1 cut(s) 816
BmeT110I CYCGRG 3 cut(s) 1055, 2113, 2333
BmgBI CACGTC 1 cut(s) 2504
BmgT120I GGNCC 2 cut(s) 2245, 2246
BmiI GGNNCC 3 cut(s) 1053, 2246, 2247
BmrFI CCNGG 7 cut(s) 60, 500, 1056, 1057, 1784, 2114, 2115
BmsI GCATC 5 cut(s) 898, 952, 1024, 1254, 1908
BmtI GCTAGC 1 cut(s) 2562
BpiI GAAGAC 4 cut(s) 839, 933, 1584, 1718
BpmI CTGGAG 2 cut(s) 467, 2759
Bpu10I CCTNAGC 2 cut(s) 54, 1636
Bpu1102I GCTNAGC 1 cut(s) 2379
Bpu14I TTCGAA 2 cut(s) 1321, 2418
BpuEI CTTGAG 3 cut(s) 817, 1520, 2441
BpuMI CCSGG 6 cut(s) 60, 500, 1056, 1057, 2114, 2115
BsaBI GATNNNNATC 2 cut(s) 2163, 2232
BsaI GGTCTC 1 cut(s) 1882
BsaWI WCCGGW 3 cut(s) 1106, 1462, 2048
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 487, 2507, 2545
Bse1I ACTGG 1 cut(s) 366
Bse3DI GCAATG 2 cut(s) 2197, 2733
Bse8I GATNNNNATC 2 cut(s) 2163, 2232
BseAI TCCGGA 1 cut(s) 1462
BseBI CCWGG 1 cut(s) 1784
BseJI GATNNNNATC 2 cut(s) 2163, 2232
BseLI CCNNNNNNNGG 4 cut(s) 59, 487, 2507, 2545
BseMI GCAATG 2 cut(s) 2197, 2733
BseMII CTCAG 9 cut(s) 45, 869, 983, 1187, 1997, 2251, 2370, 2394, 2694
BseNI ACTGG 1 cut(s) 366
BseRI GAGGAG 1 cut(s) 2168
BseSI GKGCMC 3 cut(s) 1056, 2249, 2320
BseXI GCAGC 4 cut(s) 589, 762, 1169, 1534
BseYI CCCAGC 1 cut(s) 1787
BshFI GGCC 2 cut(s) 804, 2247
BshNI GGYRCC 1 cut(s) 1051
BsiHKAI GWGCWC 2 cut(s) 1118, 2320
BsiHKCI CYCGRG 3 cut(s) 1055, 2113, 2333
BsiSI CCGG 7 cut(s) 59, 500, 1056, 1107, 1463, 2049, 2114
BslFI GGGAC 2 cut(s) 1804, 2183
BslI CCNNNNNNNGG 4 cut(s) 59, 487, 2507, 2545
BsmAI GTCTC 6 cut(s) 577, 879, 1192, 1617, 1882, 2758
BsmFI GGGAC 2 cut(s) 1804, 2183
BsmI GAATGC 1 cut(s) 1067
BsnI GGCC 2 cut(s) 804, 2247
Bso31I GGTCTC 1 cut(s) 1882
BsoBI CYCGRG 3 cut(s) 1055, 2113, 2333
Bsp119I TTCGAA 2 cut(s) 1321, 2418
Bsp120I GGGCCC 1 cut(s) 2245
Bsp1286I GDGCHC 4 cut(s) 1056, 1118, 2249, 2320
Bsp13I TCCGGA 1 cut(s) 1462
Bsp1720I GCTNAGC 1 cut(s) 2379
BspACI CCGC 3 cut(s) 115, 412, 2461
BspANI GGCC 2 cut(s) 804, 2247
BspCNI CTCAG 9 cut(s) 46, 870, 982, 1188, 1996, 2250, 2371, 2395, 2695
BspEI TCCGGA 1 cut(s) 1462
BspLI GGNNCC 3 cut(s) 1053, 2246, 2247
BspMAI CTGCAG 2 cut(s) 1533, 1902
BspMI ACCTGC 6 cut(s) 63, 533, 1039, 1522, 1536, 1891
BspOI GCTAGC 1 cut(s) 2562
BspPI GGATC 3 cut(s) 474, 2643, 2725
BspT104I TTCGAA 2 cut(s) 1321, 2418
BspT107I GGYRCC 1 cut(s) 1051
BspTNI GGTCTC 1 cut(s) 1882
BsrDI GCAATG 2 cut(s) 2197, 2733
BsrI ACTGG 1 cut(s) 366
BssNAI GTATAC 1 cut(s) 1235
BssSI CACGAG 2 cut(s) 138, 622
BssT1I CCWWGG 3 cut(s) 178, 1077, 2031
Bst1107I GTATAC 1 cut(s) 1235
Bst2BI CACGAG 2 cut(s) 138, 622
Bst2UI CCWGG 1 cut(s) 1784
Bst4CI ACNGT 4 cut(s) 426, 1845, 2130, 2411
BstAPI GCANNNNNTGC 2 cut(s) 10, 1528
BstBI TTCGAA 2 cut(s) 1321, 2418
BstC8I GCNNGC 8 cut(s) 197, 251, 656, 829, 1295, 1440, 1444, 2560
BstDSI CCRYGG 1 cut(s) 119
BstENI CCTNNNNNAGG 1 cut(s) 2543
BstHHI GCGC 2 cut(s) 249, 2204
BstMAI GTCTC 6 cut(s) 577, 879, 1192, 1617, 1882, 2758
BstMWI GCNNNNNNNGC 8 cut(s) 10, 801, 861, 1012, 1160, 1528, 2315, 2555
BstNI CCWGG 1 cut(s) 1784
BstNSI RCATGY 1 cut(s) 2110
BstSCI CCNGG 7 cut(s) 58, 498, 1054, 1055, 1782, 2112, 2113
BstSFI CTRYAG 7 cut(s) 14, 1529, 1851, 1898, 1944, 2126, 2472
BstSLI GKGCMC 3 cut(s) 1056, 2249, 2320
BstV1I GCAGC 4 cut(s) 589, 762, 1169, 1534
BstV2I GAAGAC 4 cut(s) 839, 933, 1584, 1718
BstX2I RGATCY 6 cut(s) 466, 1894, 2122, 2635, 2717, 2742
BstXI CCANNNNNNTGG 1 cut(s) 579
BstYI RGATCY 6 cut(s) 466, 1894, 2122, 2635, 2717, 2742
BstZ17I GTATAC 1 cut(s) 1235
BsuRI GGCC 2 cut(s) 804, 2247
BtgI CCRYGG 1 cut(s) 119
BtrI CACGTC 1 cut(s) 2504
BtsIMutI CAGTG 4 cut(s) 422, 1193, 2259, 2416
BveI ACCTGC 6 cut(s) 63, 533, 1039, 1522, 1536, 1891
Cac8I GCNNGC 8 cut(s) 197, 251, 656, 829, 1295, 1440, 1444, 2560
CaiI CAGNNNCTG 1 cut(s) 1528
CfoI GCGC 2 cut(s) 249, 2204
Cfr13I GGNCC 2 cut(s) 2245, 2246
Cfr9I CCCGGG 2 cut(s) 1055, 2113
CseI GACGC 1 cut(s) 995
Csp6I GTAC 4 cut(s) 475, 1495, 1722, 1825
CviAII CATG 7 cut(s) 5, 98, 338, 1492, 1603, 2107, 2615
CviQI GTAC 4 cut(s) 475, 1495, 1722, 1825
DraI TTTAAA 2 cut(s) 870, 1729
DriI GACNNNNNGTC 1 cut(s) 816
Eam1105I GACNNNNNGTC 1 cut(s) 816
Ecl136II GAGCTC 1 cut(s) 1116
Eco130I CCWWGG 3 cut(s) 178, 1077, 2031
Eco24I GRGCYC 2 cut(s) 1118, 2249
Eco31I GGTCTC 1 cut(s) 1882
Eco53kI GAGCTC 1 cut(s) 1116
Eco57I CTGAAG 8 cut(s) 490, 1031, 1176, 1283, 1502, 2235, 2604, 2632
Eco88I CYCGRG 3 cut(s) 1055, 2113, 2333
EcoICRI GAGCTC 1 cut(s) 1116
EcoNI CCTNNNNNAGG 1 cut(s) 2543
EcoO109I RGGNCCY 2 cut(s) 2245, 2246
EcoRI GAATTC 2 cut(s) 91, 2477
EcoRII CCWGG 1 cut(s) 1782
EcoT14I CCWWGG 3 cut(s) 178, 1077, 2031
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1118, 2249
ErhI CCWWGG 3 cut(s) 178, 1077, 2031
FaeI CATG 7 cut(s) 8, 101, 341, 1495, 1606, 2110, 2618
FalI AAGNNNNNCTT 2 cut(s) 1957, 1989
FaqI GGGAC 2 cut(s) 1804, 2183
FatI CATG 7 cut(s) 4, 97, 337, 1491, 1602, 2106, 2614
FauNDI CATATG 2 cut(s) 169, 1388
FbaI TGATCA 2 cut(s) 1362, 2227
FblI GTMKAC 1 cut(s) 1234
Fnu4HI GCNGC 6 cut(s) 115, 412, 603, 776, 1183, 1523
FriOI GRGCYC 2 cut(s) 1118, 2249
Fsp4HI GCNGC 6 cut(s) 115, 412, 603, 776, 1183, 1523
FspBI CTAG 7 cut(s) 350, 629, 698, 1344, 2516, 2559, 2687
FspI TGCGCA 1 cut(s) 248
GlaI GCGC 2 cut(s) 248, 2203
GluI GCNGC 6 cut(s) 115, 412, 603, 776, 1183, 1523
GsaI CCCAGC 1 cut(s) 1791
GsuI CTGGAG 2 cut(s) 467, 2759
HaeIII GGCC 2 cut(s) 804, 2247
HapII CCGG 7 cut(s) 59, 500, 1056, 1107, 1463, 2049, 2114
HgaI GACGC 1 cut(s) 995
HhaI GCGC 2 cut(s) 249, 2204
Hin1II CATG 7 cut(s) 8, 101, 341, 1495, 1606, 2110, 2618
Hin6I GCGC 2 cut(s) 247, 2202
HinP1I GCGC 2 cut(s) 247, 2202
HincII GTYRAC 3 cut(s) 280, 534, 1848
HindII GTYRAC 3 cut(s) 280, 534, 1848
HindIII AAGCTT 3 cut(s) 853, 956, 1295
HpaII CCGG 7 cut(s) 59, 500, 1056, 1107, 1463, 2049, 2114
HphI GGTGA 4 cut(s) 142, 560, 2237, 2420
Hpy166II GTNNAC 7 cut(s) 280, 534, 568, 1235, 1848, 2318, 2678
Hpy8I GTNNAC 7 cut(s) 280, 534, 568, 1235, 1848, 2318, 2678
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 5 cut(s) 244, 1002, 1151, 1317, 2259
HpyCH4III ACNGT 4 cut(s) 426, 1845, 2130, 2411
HpyCH4IV ACGT 3 cut(s) 812, 2320, 2503
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 801, 861, 1012, 1160, 1528, 2315, 2555
HpySE526I ACGT 3 cut(s) 812, 2320, 2503
Hsp92II CATG 7 cut(s) 8, 101, 341, 1495, 1606, 2110, 2618
HspAI GCGC 2 cut(s) 247, 2202
Kpn2I TCCGGA 1 cut(s) 1462
Ksp22I TGATCA 2 cut(s) 1362, 2227
LmnI GCTCC 3 cut(s) 936, 1160, 2051
Lsp1109I GCAGC 4 cut(s) 589, 762, 1169, 1534
LweI GCATC 5 cut(s) 898, 952, 1024, 1254, 1908
MaeI CTAG 7 cut(s) 350, 629, 698, 1344, 2516, 2559, 2687
MaeII ACGT 3 cut(s) 812, 2320, 2503
MaeIII GTNAC 6 cut(s) 148, 318, 1412, 1679, 2265, 2534
MfeI CAATTG 2 cut(s) 218, 1088
MflI RGATCY 6 cut(s) 466, 1894, 2122, 2635, 2717, 2742
MhlI GDGCHC 4 cut(s) 1056, 1118, 2249, 2320
MlyI GAGTC 3 cut(s) 432, 579, 634
MmeI TCCRAC 1 cut(s) 1191
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 1462
MroXI GAANNNNTTC 2 cut(s) 1512, 2424
MslI CAYNNNNRTG 1 cut(s) 1607
MspA1I CMGCKG 1 cut(s) 2088
MspI CCGG 7 cut(s) 59, 500, 1056, 1107, 1463, 2049, 2114
MspR9I CCNGG 7 cut(s) 60, 500, 1056, 1057, 1784, 2114, 2115
MunI CAATTG 2 cut(s) 218, 1088
Mva1269I GAATGC 1 cut(s) 1067
MvaI CCWGG 1 cut(s) 1784
MwoI GCNNNNNNNGC 8 cut(s) 10, 801, 861, 1012, 1160, 1528, 2315, 2555
NciI CCSGG 6 cut(s) 60, 500, 1056, 1057, 2114, 2115
NdeI CATATG 2 cut(s) 169, 1388
NheI GCTAGC 1 cut(s) 2558
NlaIII CATG 7 cut(s) 8, 101, 341, 1495, 1606, 2110, 2618
NlaIV GGNNCC 3 cut(s) 1053, 2246, 2247
NmeAIII GCCGAG 1 cut(s) 2594
NmuCI GTSAC 3 cut(s) 148, 1412, 2534
NsbI TGCGCA 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 2110
NspV TTCGAA 2 cut(s) 1321, 2418
PaeR7I CTCGAG 1 cut(s) 2333
PaqCI CACCTGC 2 cut(s) 1039, 1536
PctI GAATGC 1 cut(s) 1067
PdmI GAANNNNTTC 2 cut(s) 1512, 2424
PfeI GAWTC 9 cut(s) 47, 157, 353, 430, 1073, 1165, 2338, 2365, 2734
PflFI GACNNNGTC 1 cut(s) 441
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 6 cut(s) 116, 413, 604, 777, 1184, 1524
PleI GAGTC 3 cut(s) 432, 579, 633
PpsI GAGTC 3 cut(s) 432, 579, 633
Psp124BI GAGCTC 1 cut(s) 1118
Psp6I CCWGG 1 cut(s) 1782
PspFI CCCAGC 1 cut(s) 1787
PspGI CCWGG 1 cut(s) 1782
PspN4I GGNNCC 3 cut(s) 1053, 2246, 2247
PspOMI GGGCCC 1 cut(s) 2245
PspPI GGNCC 2 cut(s) 2245, 2246
PstI CTGCAG 2 cut(s) 1533, 1902
PstNI CAGNNNCTG 1 cut(s) 1528
PsuI RGATCY 6 cut(s) 466, 1894, 2122, 2635, 2717, 2742
PsyI GACNNNGTC 1 cut(s) 441
PvuII CAGCTG 1 cut(s) 2088
RsaI GTAC 4 cut(s) 476, 1496, 1723, 1826
RsaNI GTAC 4 cut(s) 475, 1495, 1722, 1825
RseI CAYNNNNRTG 1 cut(s) 1607
SacI GAGCTC 1 cut(s) 1118
SatI GCNGC 6 cut(s) 115, 412, 603, 776, 1183, 1523
Sau96I GGNCC 2 cut(s) 2245, 2246
SbfI CCTGCAGG 1 cut(s) 1533
ScaI AGTACT 1 cut(s) 1826
SchI GAGTC 3 cut(s) 432, 579, 634
ScrFI CCNGG 7 cut(s) 60, 500, 1056, 1057, 1784, 2114, 2115
SdaI CCTGCAGG 1 cut(s) 1533
SduI GDGCHC 4 cut(s) 1056, 1118, 2249, 2320
SfaNI GCATC 5 cut(s) 898, 952, 1024, 1254, 1908
SfcI CTRYAG 7 cut(s) 14, 1529, 1851, 1898, 1944, 2126, 2472
Sfr274I CTCGAG 1 cut(s) 2333
SfuI TTCGAA 2 cut(s) 1321, 2418
SlaI CTCGAG 1 cut(s) 2333
SmaI CCCGGG 2 cut(s) 1057, 2115
SmiMI CAYNNNNRTG 1 cut(s) 1607
SmlI CTYRAG 4 cut(s) 796, 1499, 2333, 2456
SmoI CTYRAG 4 cut(s) 796, 1499, 2333, 2456
Sse8387I CCTGCAGG 1 cut(s) 1533
SsiI CCGC 3 cut(s) 115, 412, 2461
SspMI CTAG 7 cut(s) 350, 629, 698, 1344, 2516, 2559, 2687
SstI GAGCTC 1 cut(s) 1118
StyD4I CCNGG 7 cut(s) 58, 498, 1054, 1055, 1782, 2112, 2113
StyI CCWWGG 3 cut(s) 178, 1077, 2031
TaaI ACNGT 4 cut(s) 426, 1845, 2130, 2411
TaiI ACGT 3 cut(s) 815, 2323, 2506
TaqI TCGA 8 cut(s) 373, 390, 1321, 1861, 2334, 2418, 2437, 2762
TaqII GACCGA 2 cut(s) 833, 1752
TatI WGTACW 2 cut(s) 1721, 1824
TauI GCSGC 2 cut(s) 117, 414
TfiI GAWTC 9 cut(s) 47, 157, 353, 430, 1073, 1165, 2338, 2365, 2734
TscAI CASTG 4 cut(s) 429, 1200, 2259, 2416
TseFI GTSAC 3 cut(s) 148, 1412, 2534
TseI GCWGC 4 cut(s) 602, 775, 1182, 1522
Tsp45I GTSAC 3 cut(s) 148, 1412, 2534
TspGWI ACGGA 2 cut(s) 2003, 2588
TspMI CCCGGG 2 cut(s) 1055, 2113
TspRI CASTG 4 cut(s) 429, 1200, 2259, 2416
Tth111I GACNNNGTC 1 cut(s) 441
Van91I CCANNNNNTGG 1 cut(s) 487
VneI GTGCAC 1 cut(s) 2316
XagI CCTNNNNNAGG 1 cut(s) 2543
XbaI TCTAGA 4 cut(s) 349, 628, 2515, 2686
XceI RCATGY 1 cut(s) 2110
XhoI CTCGAG 1 cut(s) 2333
XmaI CCCGGG 2 cut(s) 1055, 2113
XmiI GTMKAC 1 cut(s) 1234
XmnI GAANNNNTTC 2 cut(s) 1512, 2424
XspI CTAG 7 cut(s) 350, 629, 698, 1344, 2516, 2559, 2687
ZrmI AGTACT 1 cut(s) 1826
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.