RchiOBHm_Chr2g0124621

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000307
Physical Location & Seq
Forward (+)
37894510 .. 37900266
5757 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49678

Sequence Viewer

Length: 5133 bp
ATGCCTAACAGCCGCATTGAACAACTATGGAATGAAACGCTTTCTCTGAAAATGCTTATACTCATGGATCTTTCTGACTGCAAGTATTTAACCAAGACCCTGGACTTCAGTAAGGTCCCAAAGCTTGAGAAATTGATCCTTAAAGGTTGTATAGAGTTATTGGAGGTTCACCCTACTATTGGGGATCTCCAACATTTGGTTTTATTGAACTTGAAAGGTTGTGAAAGTCTAGAGAACCTTCCTCAATCCATCAGGTTGAGATCTCTTCGAACTTTTATTCTTTCGGGATGTTCAAAACTCAGACACTTTCCAGAGATTGTGGGGAACATGGATACTTTATCAGAACTTTATTTAGATGGCACGGCTATACGGGAGCTGCCTGTATCAATCCAGCATTTGGAAGGCCTCATTTTACTTAATCTAAGTGGGTGCAGGAACCTTCTCAGTGTTCCAAGCATCCTTTCTAGTAGTTTGACATCACTGAAATTTCTCTATCTGTCACTATGCTCACGTATGGACAGACTGCCGGATAACATAGGCTGCTTGGAACACTTGGAGGAGCTTGATGCGTGTAATATTGCTATAAGAAAAGTGCCCGATTCCATTTTTCTCCTCAAGAATCTTAAATTATTGTGTTTCCATGGCTGTGCTGGATCCACAGGTTTAGAGTTGCCAAGCAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGAGCAATCCCCAATGACATTGGTGATTTATTCTCACTGCAGAGTCTGGATTTAAGTGAGAACAACTTTTTCACCATACCTGAAAGCATCTCTCAGCTTTCTGAGCTTACAGAAATTTCTCTGTTTAAGTGTAGCAAACTATGGACGTTGCCAAAAGATCTTCCGTCAAGTCTAAGAAATTTAAATGTACGAGGTTGTCCTATGCTGACAAGTTCTTCATCTAGTTGGAGGAGATATCCGCCTCAAAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGAGGATGAAATCTTTCCCGAACTCTATAAGGTTCCATTCTCTCTCTTAATCACACCGATGTTAAAAAGAAAAATCTTTTACTCTAAGTATAGCTGTATATATATGCTTTTCTTGAATCATATTGTCATCAAGTTCAACAGTGCTTTTCTTTCAGGTATTTATCTAATTACTCTCTTTTATACGCACATGCAGTTTCGTTTGGGAAATCTGGAAGAACTCGATCTTTCCAACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAATTGAACCTTGAAGGTTGTGAAAAATTATCAGAGGTTCACCCAACCATTGGGTGTCTCCAACATTTGGTGTTTCTGAATTTGAAAGGATGTGTAAATCTAGAGAGCCTTCCCAGTTCCATCAGCTTGAAGTCTCTAAACATGTTTGTTCTTTCGGGATGTTCAAAGCTGAAAGAGTTTCCAAACATTAAGGGAGAAATGAACAATTTGTCACAACTGCATTTAGATGGGACGGCGTTAAGGCATCTGCGAATTCCGATGATGCATTTGAAAGGCCCAATTTTGATAAATCTGTCAGGCTGCAGGAACCTTTTGACTGTTCCAATCCTATTCAGTCTAAAATCTCTCAATCTATCACGGTGCTCAACTATATTCAAATTGCCACCGAGCCTGGCATACATGGAACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACAGGATTACCCCAGTCCATTTTACTCTTGAAGAAACTTAAAGTGTTATCTTTCTGTGGATGCAAAGGTTTGCAATTGCCTAAATGGTTCTCGGATTTAAGCTCTTTGACGTCATTAAATCTACGGAGGTGTAGTCTAGCAGAAGAAGTCCTTGATAGCCTCTACAGCTTATCTGCTCTGCAGATTTTGGATTTGAGTGAAAACAATCTTTCGATCATACCCAGTGGAATTGGTCGCTTATCCTCTTTGAAGCTATTGAATTTGAGTGAGAACAATTTTGACAATATACCAAATGAAATCGGTCACTTATCCTCTTTGCAGGTCTTAGATTTGAGTGAAAACAGCTTCATGAGTATACCTGATGAGAGCATCTCTCATCTTTCTGAGCTTACAGAACTACGCTTGTTTAGATGTAGGAAGCTACAGTCCTTGCCGAATAATCTTCCCTTCAATCTAAAACATGTCCATGCACGAGAATGTCCTATGTTGAAGAATAATGCAGATACTTTGACAATATGGGCATCTGGAAAGGGGTTCTGTTTCATAAATTGCAGACAATCTGACCAGGATGATGGTCAGCCAAATCACCTCCCAGTTCCAGTTCCCAAAGACCGTATTGAACTACTCTTTCCTTCATACATTGAGGACCGAGTGTATGGCAAAAAACCATTTGAAATTCGTTTTCCACATTCGTGGAGTACAGGACTGAGTGCCGGAATTCCAAATTCGTGGAGTCGTTGGAGAAATGGCCCTTCCGTGACAATCCCACTCTCTGATGGTAACAGTACGTGGATGGGACTTGCTCTTTTTGTTGTTTTTGATATCCTTGAGCAAGACATTTTCAACAAGAGCTGGGAATTGGAGGAGACAGTCTGTGATTTTCACACCGACGTTGGGGCGGTTGGGCATGATACTTCCCTAGTTTTTCAAAACTTCATAGACTTCAGGACTGGGTCATATGGTCTTAGTTGCTATGAACCACGTGGTGGGCAATTTAGTGGGTTGTTCGATAAACCAAGTTCACGACTTCGAGCTTCAGTTTCAACAAAGAGACCAAATTTAAAAGTTAGAGGTTGTGGGATACATCTAATATCAGAGGAATATGCTGCAGAGTTTGTTAAAAGTATAGCAACTCAGACTACTAATTCAATTGAGAGTCATCTTGATTCCAATTTTGATCGACATTGCGAGGACATATTAGATGAGGAAACAACTGGTGTCCTGATAGAACAGGGATCTACTAATTCAACTTTCAATGAAGATAGCTGCAGCAAAGTTAACTCTAAGATCAAAATCAGAGGAGAGCTGTCGATACTCTATGAGGGAAGCAAGGGACGTCAGAAGAGTTTCCACTTTTGCTTTCCTGCTTCAGTAATTTCAACCCTGCCTTGGTTCTTCCATCACCATGCAGGGGATGTAACATTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCGACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTATGCTCTCATGATCATGGAAGTATGGTAATGCACGGATCACTCAAGATAAAGAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAGGGTATCAGCGCATTGTTCAGAACCATTAATGAAGAAATGGAGGTCCAAGTGTGTGGAAGCCGTTTAGCATTTGAGCATGATTTGGAAGACTTGACCCATTCATTAACTGCTGCTGCCAGCACGTTGGGGCAACATGCCCTCACTCAACTATGTTCCCAGGCCCAACCTGTTGATCGGCGCAATGCAGAGGAAGCAGAAATGCCCTTCAATTGCTGTTCCTGGTTTAGAAGAAGTACTGCACTTGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATCACAGATGCTATCTCCAGCAACAGCAAGGTTTTGGGGAGTCTGACCTTACCCAATTGGTCCACTCCAGATCAGTAACATTACTCCACTCGAAAAGCCTTTTGGAAAACAGGGATAGAAACCATGAAAACGTTGCCGAAGAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGATATGGTTCACCACGGTGGAAGAGATGCCTGAAGTTGTTGCTTCGACAGTCCAAGGTGGCTACTCTCAGTCTTTGTGGACACGCAATTTCAGCTTTCAAGAATTTTGATCCTTTCTCCCCATACAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAACTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCGTCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCATATCCCTATGTGTTGCATCAATGAAGAGAAACTCAAGTCGCTGCATCTTGGTAGATTCATTTGGCTAACCTACATTCCCCGTCTTTTGCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAGAGGCTTGACAGTAGAGAAGAGTGGTATACGTCTCTTGTACAGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAGTGCTGGACATCTTTCTTTGATAATCTGTCTTTCATCCGTCAACTAGTAGAATCAGATGATCAAAATATTCAACCAAGGATAAGACATGAACTTCCGATGCTTGAAGGTCATATCAAGGTTTTTGAACCGGATTTAATATATAATGCAATCCCCCCGTCTAATGAAATTCCGGAGTGGTTCGGGCACCGCATTGAGGACCCCTGGGATCCCAGCTGTGGCTGGCAATTCCAGTTACCACCACCTTTGAGTGACACAATAGGATTAGCTCTCTTTGTGTCATATCGTATTAGCAGAAATTATTTGCAGGAAAGTGTTTTGTACCCCTTTATTATAACATTGAAAACTGCGAAGAATGGTTTGTCCTCTCTCCATCGGTATCAAATGAGCAATGAAGAATATGAGTTCCTAAAGCGCTGTTGTACTGTGCATCGTGAATTTTTCTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAACTAAATGATGAGTCTGACCTCATAGTTTTTTCTGGTCGGAACTGCTGGACGCCGGACATGGTCTATCTCCGTTTTGTGTATGCGGATGAGGTGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGTCCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

1710

Amino Acids

194.57

Weight (kDa)

6.55

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 57 - 164 9.8e-07 Leucine-rich repeat region
LRR_13 PF23286 86 - 154 2.9e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 139 - 239 3.8e-10 Leucine-rich repeat region
LRR_4 PF12799 409 - 446 1.6e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 531 - 647 3.8e-11 Leucine-rich repeat region
LRR_14 PF23598 630 - 727 4.9e-12 Leucine-rich repeat region
LRR_8 PF13855 663 - 719 1e-07 Leucine rich repeat
C-JID PF20160 1355 - 1492 3.7e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4838
AatII GACGTC 2 cut(s) 1856, 3082
Acc36I ACCTGC 1 cut(s) 2053
AccB1I GGYRCC 1 cut(s) 4689
AccB7I CCANNNNNTGG 5 cut(s) 196, 397, 1352, 1369, 2729
AccI GTMKAC 2 cut(s) 2098, 4453
AccIII TCCGGA 1 cut(s) 4675
AciI CCGC 6 cut(s) 13, 977, 2642, 3706, 4693, 5069
AclI AACGTT 1 cut(s) 3846
AcuI CTGAAG 6 cut(s) 91, 2671, 2763, 3096, 3899, 3974
AcvI CACGTG 2 cut(s) 2726, 3392
AcyI GRCGYC 3 cut(s) 1853, 3079, 5036
AdeI CACNNNGTG 1 cut(s) 2729
AfeI AGCGCT 1 cut(s) 4919
AflII CTTAAG 1 cut(s) 1298
AflIII ACRYGT 4 cut(s) 1443, 2203, 3382, 3391
AhdI GACNNNNNGTC 1 cut(s) 5120
AhlI ACTAGT 1 cut(s) 4549
AjnI CCWGG 6 cut(s) 99, 1692, 2307, 3582, 3644, 4706
AjuI GAANNNNNNNTTGG 4 cut(s) 445, 477, 3800, 3832
AleI CACNNNNGTG 2 cut(s) 2434, 3937
Alw21I GWGCWC 1 cut(s) 1667
Alw26I GTCTC 6 cut(s) 1364, 1440, 2603, 2788, 3899, 4463
AlwNI CAGNNNCTG 1 cut(s) 784
Aor13HI TCCGGA 1 cut(s) 4675
Aor51HI AGCGCT 1 cut(s) 4919
AoxI GGCC 5 cut(s) 403, 1576, 2491, 3585, 4404
AseI ATTAAT 1 cut(s) 3453
Asp700I GAANNNNTTC 2 cut(s) 1032, 3089
AspA2I CCTAGG 1 cut(s) 715
AspLEI GCGC 3 cut(s) 3437, 3606, 4920
AspS9I GGNCC 9 cut(s) 115, 1577, 2389, 2492, 3357, 3469, 3586, 3775, 4702
AsuC2I CCSGG 1 cut(s) 4232
AsuHPI GGTGA 7 cut(s) 161, 773, 802, 1334, 2321, 3137, 3924
AsuII TTCGAA 1 cut(s) 268
AvaII GGWCC 6 cut(s) 115, 2389, 3357, 3469, 3775, 4702
AvrII CCTAGG 1 cut(s) 715
BaeGI GKGCMC 2 cut(s) 597, 4692
BamHI GGATCC 2 cut(s) 653, 4711
BanI GGYRCC 1 cut(s) 4689
BarI GAAGNNNNNNTAC 2 cut(s) 3235, 3267
BauI CACGAG 2 cut(s) 2214, 4157
BbrPI CACGTG 2 cut(s) 2726, 3392
BbsI GAAGAC 2 cut(s) 3519, 4107
Bbv12I GWGCWC 1 cut(s) 1667
BceAI ACGGC 3 cut(s) 378, 1551, 3471
BciT130I CCWGG 6 cut(s) 101, 1694, 2309, 3584, 3646, 4708
BciVI GTATCC 2 cut(s) 325, 2817
BclI TGATCA 3 cut(s) 3196, 3304, 4564
BcnI CCSGG 1 cut(s) 4232
BcoDI GTCTC 6 cut(s) 1364, 1440, 2603, 2788, 3899, 4463
BcuI ACTAGT 1 cut(s) 4549
BfmI CTRYAG 7 cut(s) 776, 1603, 1906, 1922, 2165, 2850, 3010
BfoI RGCGCY 1 cut(s) 4921
BfrI CTTAAG 1 cut(s) 1298
BfuAI ACCTGC 1 cut(s) 2053
BfuI GTATCC 2 cut(s) 325, 2817
BglII AGATCT 3 cut(s) 260, 695, 895
BlnI CCTAGG 1 cut(s) 715
BmcAI AGTACT 1 cut(s) 3661
Bme1390I CCNGG 7 cut(s) 101, 1694, 2309, 3584, 3646, 4232, 4708
Bme18I GGWCC 6 cut(s) 115, 2389, 3357, 3469, 3775, 4702
BmeRI GACNNNNNGTC 1 cut(s) 5120
BmgT120I GGNCC 9 cut(s) 115, 1577, 2389, 2492, 3357, 3469, 3586, 3775, 4702
BmrFI CCNGG 7 cut(s) 101, 1694, 2309, 3584, 3646, 4232, 4708
BmrI ACTGGG 5 cut(s) 1410, 1750, 1959, 2330, 2703
BmuI ACTGGG 5 cut(s) 1410, 1750, 1959, 2330, 2703
BoxI GACNNNNGTC 1 cut(s) 4215
BpiI GAAGAC 2 cut(s) 3519, 4107
BplI GAGNNNNNCTC 4 cut(s) 2099, 2131, 2101, 2133
BpmI CTGGAG 3 cut(s) 3716, 3766, 4192
Bpu14I TTCGAA 1 cut(s) 268
BpuEI CTTGAG 6 cut(s) 146, 599, 2591, 3320, 4273, 4301
BpuMI CCSGG 1 cut(s) 4232
BsaAI YACGTR 4 cut(s) 512, 2532, 2726, 3392
BsaBI GATNNNNATC 1 cut(s) 3035
BsaHI GRCGYC 3 cut(s) 1853, 3079, 5036
BsaI GGTCTC 1 cut(s) 2788
BsaWI WCCGGW 2 cut(s) 4633, 4675
BsaXI ACNNNNNCTCC 2 cut(s) 1861, 1891
Bse3DI GCAATG 3 cut(s) 2926, 3613, 4900
Bse8I GATNNNNATC 1 cut(s) 3035
BseAI TCCGGA 1 cut(s) 4675
BseBI CCWGG 6 cut(s) 101, 1694, 2309, 3584, 3646, 4708
BseJI GATNNNNATC 1 cut(s) 3035
BseMI GCAATG 3 cut(s) 2926, 3613, 4900
BseRI GAGGAG 7 cut(s) 572, 602, 982, 1034, 1730, 2621, 3057
BseSI GKGCMC 2 cut(s) 597, 4692
BseYI CCCAGC 2 cut(s) 2595, 4715
BsgI GTGCAG 2 cut(s) 451, 3648
BshFI GGCC 5 cut(s) 405, 1578, 2493, 3587, 4406
BshNI GGYRCC 1 cut(s) 4689
BsiHKAI GWGCWC 1 cut(s) 1667
BsiSI CCGG 6 cut(s) 527, 2457, 4232, 4634, 4676, 5039
BslFI GGGAC 5 cut(s) 101, 1546, 2553, 3090, 3370
BsmAI GTCTC 6 cut(s) 1364, 1440, 2603, 2788, 3899, 4463
BsmBI CGTCTC 1 cut(s) 4463
BsmFI GGGAC 5 cut(s) 101, 1546, 2553, 3090, 3370
BsnI GGCC 5 cut(s) 405, 1578, 2493, 3587, 4406
Bso31I GGTCTC 1 cut(s) 2788
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 597, 1667, 4692
Bsp13I TCCGGA 1 cut(s) 4675
Bsp1407I TGTACA 3 cut(s) 3242, 3889, 4464
Bsp19I CCATGG 1 cut(s) 640
BspACI CCGC 6 cut(s) 13, 977, 2642, 3706, 4693, 5069
BspANI GGCC 5 cut(s) 405, 1578, 2493, 3587, 4406
BspEI TCCGGA 1 cut(s) 4675
BspHI TCATGA 3 cut(s) 2091, 3301, 4207
BspMAI CTGCAG 5 cut(s) 780, 1607, 1926, 2854, 3014
BspMI ACCTGC 1 cut(s) 2053
BspT104I TTCGAA 1 cut(s) 268
BspT107I GGYRCC 1 cut(s) 4689
BspTI CTTAAG 1 cut(s) 1298
BspTNI GGTCTC 1 cut(s) 2788
BsrDI GCAATG 3 cut(s) 2926, 3613, 4900
BsrGI TGTACA 3 cut(s) 3242, 3889, 4464
BssNAI GTATAC 2 cut(s) 2099, 4454
BssNI GRCGYC 3 cut(s) 1853, 3079, 5036
BssSI CACGAG 2 cut(s) 2214, 4157
BssT1I CCWWGG 6 cut(s) 640, 715, 3131, 3975, 4260, 4580
Bst1107I GTATAC 2 cut(s) 2099, 4454
Bst2BI CACGAG 2 cut(s) 2214, 4157
Bst2UI CCWGG 6 cut(s) 101, 1694, 2309, 3584, 3646, 4708
Bst6I CTCTTC 7 cut(s) 270, 3080, 3938, 4302, 4439, 4470, 5076
BstACI GRCGYC 3 cut(s) 1853, 3079, 5036
BstAFI CTTAAG 1 cut(s) 1298
BstAUI TGTACA 3 cut(s) 3242, 3889, 4464
BstBAI YACGTR 4 cut(s) 512, 2532, 2726, 3392
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 3544, 4727
BstDSI CCRYGG 2 cut(s) 640, 3935
BstH2I RGCGCY 1 cut(s) 4921
BstHHI GCGC 3 cut(s) 3437, 3606, 4920
BstMAI GTCTC 6 cut(s) 1364, 1440, 2603, 2788, 3899, 4463
BstMWI GCNNNNNNNGC 5 cut(s) 740, 841, 1908, 3617, 4013
BstNI CCWGG 6 cut(s) 101, 1694, 2309, 3584, 3646, 4708
BstNSI RCATGY 5 cut(s) 1210, 1447, 2207, 3386, 3563
BstPAI GACNNNNGTC 1 cut(s) 4215
BstSCI CCNGG 7 cut(s) 99, 1692, 2307, 3582, 3644, 4230, 4706
BstSFI CTRYAG 7 cut(s) 776, 1603, 1906, 1922, 2165, 2850, 3010
BstSLI GKGCMC 2 cut(s) 597, 4692
BstV2I GAAGAC 2 cut(s) 3519, 4107
BstX2I RGATCY 9 cut(s) 67, 184, 260, 653, 695, 895, 1269, 2978, 4711
BstXI CCANNNNNNTGG 7 cut(s) 100, 2315, 2436, 2472, 3479, 3550, 4971
BstYI RGATCY 9 cut(s) 67, 184, 260, 653, 695, 895, 1269, 2978, 4711
BstZ17I GTATAC 2 cut(s) 2099, 4454
BsuI GTATCC 2 cut(s) 325, 2817
BsuRI GGCC 5 cut(s) 405, 1578, 2493, 3587, 4406
BtgI CCRYGG 2 cut(s) 640, 3935
BtsI GCAGTG 1 cut(s) 773
BtsIMutI CAGTG 7 cut(s) 451, 479, 773, 1165, 1972, 4086, 4390
BveI ACCTGC 1 cut(s) 2053
Cac8I GCNNGC 2 cut(s) 3544, 4727
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 3 cut(s) 2091, 3301, 4207
CfoI GCGC 3 cut(s) 3437, 3606, 4920
Cfr13I GGNCC 9 cut(s) 115, 1577, 2389, 2492, 3357, 3469, 3586, 3775, 4702
CseI GACGC 3 cut(s) 4205, 4977, 5044
CspCI CAANNNNNGTGG 2 cut(s) 4731, 4766
DraI TTTAAA 2 cut(s) 921, 2805
DraIII CACNNNGTG 1 cut(s) 2729
DriI GACNNNNNGTC 1 cut(s) 5120
Eam1104I CTCTTC 7 cut(s) 270, 3080, 3938, 4302, 4439, 4470, 5076
Eam1105I GACNNNNNGTC 1 cut(s) 5120
EarI CTCTTC 7 cut(s) 270, 3080, 3938, 4302, 4439, 4470, 5076
EciI GGCGGA 1 cut(s) 966
Eco130I CCWWGG 6 cut(s) 640, 715, 3131, 3975, 4260, 4580
Eco147I AGGCCT 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 2788
Eco32I GATATC 2 cut(s) 974, 2566
Eco47I GGWCC 6 cut(s) 115, 2389, 3357, 3469, 3775, 4702
Eco47III AGCGCT 1 cut(s) 4919
Eco57I CTGAAG 6 cut(s) 91, 2671, 2763, 3096, 3899, 3974
Eco72I CACGTG 2 cut(s) 2726, 3392
EcoO109I RGGNCCY 3 cut(s) 115, 3357, 4702
EcoRI GAATTC 2 cut(s) 1554, 2460
EcoRII CCWGG 6 cut(s) 99, 1692, 2307, 3582, 3644, 4706
EcoRV GATATC 2 cut(s) 974, 2566
EcoT14I CCWWGG 6 cut(s) 640, 715, 3131, 3975, 4260, 4580
EcoT22I ATGCAT 1 cut(s) 1569
ErhI CCWWGG 6 cut(s) 640, 715, 3131, 3975, 4260, 4580
Esp3I CGTCTC 1 cut(s) 4463
FalI AAGNNNNNCTT 2 cut(s) 1878, 1910
FaqI GGGAC 5 cut(s) 101, 1546, 2553, 3090, 3370
FauNDI CATATG 1 cut(s) 2701
FbaI TGATCA 3 cut(s) 3196, 3304, 4564
FblI GTMKAC 2 cut(s) 2098, 4453
GlaI GCGC 3 cut(s) 3436, 3605, 4919
GsaI CCCAGC 2 cut(s) 2599, 4719
GsuI CTGGAG 3 cut(s) 3716, 3766, 4192
HaeII RGCGCY 1 cut(s) 4921
HaeIII GGCC 5 cut(s) 405, 1578, 2493, 3587, 4406
HapII CCGG 6 cut(s) 527, 2457, 4232, 4634, 4676, 5039
HgaI GACGC 3 cut(s) 4205, 4977, 5044
HhaI GCGC 3 cut(s) 3437, 3606, 4920
Hin1I GRCGYC 3 cut(s) 1853, 3079, 5036
Hin6I GCGC 3 cut(s) 3435, 3604, 4918
HinP1I GCGC 3 cut(s) 3435, 3604, 4918
HincII GTYRAC 2 cut(s) 3022, 4547
HindII GTYRAC 2 cut(s) 3022, 4547
HindIII AAGCTT 1 cut(s) 122
HpaI GTTAAC 1 cut(s) 3022
HpaII CCGG 6 cut(s) 527, 2457, 4232, 4634, 4676, 5039
HphI GGTGA 7 cut(s) 161, 773, 802, 1334, 2321, 3137, 3924
Hpy99I CGWCG 2 cut(s) 2636, 4971
HpyF10VI GCNNNNNNNGC 5 cut(s) 740, 841, 1908, 3617, 4013
Hsp92I GRCGYC 3 cut(s) 1853, 3079, 5036
HspAI GCGC 3 cut(s) 3435, 3604, 4918
Kpn2I TCCGGA 1 cut(s) 4675
Ksp22I TGATCA 3 cut(s) 3196, 3304, 4564
KspAI GTTAAC 1 cut(s) 3022
LmnI GCTCC 5 cut(s) 373, 559, 740, 1717, 3691
MfeI CAATTG 5 cut(s) 1817, 2892, 3634, 3699, 3770
MflI RGATCY 9 cut(s) 67, 184, 260, 653, 695, 895, 1269, 2978, 4711
MhlI GDGCHC 3 cut(s) 597, 1667, 4692
MlyI GAGTC 6 cut(s) 790, 2485, 2908, 3764, 3901, 5006
MmeI TCCRAC 7 cut(s) 214, 944, 1272, 1387, 2462, 4244, 5003
Mph1103I ATGCAT 1 cut(s) 1569
MroI TCCGGA 1 cut(s) 4675
MroXI GAANNNNTTC 2 cut(s) 1032, 3089
MspA1I CMGCKG 1 cut(s) 4719
MspCI CTTAAG 1 cut(s) 1298
MspI CCGG 6 cut(s) 527, 2457, 4232, 4634, 4676, 5039
MspR9I CCNGG 7 cut(s) 101, 1694, 2309, 3584, 3646, 4232, 4708
MunI CAATTG 5 cut(s) 1817, 2892, 3634, 3699, 3770
MvaI CCWGG 6 cut(s) 101, 1694, 2309, 3584, 3646, 4708
MwoI GCNNNNNNNGC 5 cut(s) 740, 841, 1908, 3617, 4013
NciI CCSGG 1 cut(s) 4232
NcoI CCATGG 1 cut(s) 640
NdeI CATATG 1 cut(s) 2701
NmuCI GTSAC 6 cut(s) 498, 1512, 2045, 2500, 4247, 4754
NsiI ATGCAT 1 cut(s) 1569
NspI RCATGY 5 cut(s) 1210, 1447, 2207, 3386, 3563
NspV TTCGAA 1 cut(s) 268
OliI CACNNNNGTG 2 cut(s) 2434, 3937
PagI TCATGA 3 cut(s) 2091, 3301, 4207
PasI CCCWGGG 1 cut(s) 4707
PceI AGGCCT 1 cut(s) 405
PciI ACATGT 3 cut(s) 1443, 2203, 3382
PdmI GAANNNNTTC 2 cut(s) 1032, 3089
PfeI GAWTC 9 cut(s) 599, 619, 1135, 2909, 3415, 4134, 4279, 4338, 4556
PflFI GACNNNGTC 2 cut(s) 2695, 5045
PflMI CCANNNNNTGG 5 cut(s) 196, 397, 1352, 1369, 2729
PleI GAGTC 6 cut(s) 789, 2484, 2907, 3763, 3901, 5005
PmaCI CACGTG 2 cut(s) 2726, 3392
PmlI CACGTG 2 cut(s) 2726, 3392
PpsI GAGTC 6 cut(s) 789, 2484, 2907, 3763, 3901, 5005
Ppu21I YACGTR 4 cut(s) 512, 2532, 2726, 3392
PpuMI RGGWCCY 3 cut(s) 115, 3357, 4702
PscI ACATGT 3 cut(s) 1443, 2203, 3382
PshAI GACNNNNGTC 1 cut(s) 4215
PshBI ATTAAT 1 cut(s) 3453
PsiI TTATAA 1 cut(s) 4838
Psp1406I AACGTT 1 cut(s) 3846
Psp5II RGGWCCY 3 cut(s) 115, 3357, 4702
Psp6I CCWGG 6 cut(s) 99, 1692, 2307, 3582, 3644, 4706
PspCI CACGTG 2 cut(s) 2726, 3392
PspFI CCCAGC 2 cut(s) 2595, 4715
PspGI CCWGG 6 cut(s) 99, 1692, 2307, 3582, 3644, 4706
PspPI GGNCC 9 cut(s) 115, 1577, 2389, 2492, 3357, 3469, 3586, 3775, 4702
PspPPI RGGWCCY 3 cut(s) 115, 3357, 4702
PsrI GAACNNNNNNTAC 2 cut(s) 3378, 3410
PstI CTGCAG 5 cut(s) 780, 1607, 1926, 2854, 3014
PstNI CAGNNNCTG 1 cut(s) 784
PsuI RGATCY 9 cut(s) 67, 184, 260, 653, 695, 895, 1269, 2978, 4711
PsyI GACNNNGTC 2 cut(s) 2695, 5045
PvuII CAGCTG 1 cut(s) 4719
Sau96I GGNCC 9 cut(s) 115, 1577, 2389, 2492, 3357, 3469, 3586, 3775, 4702
ScaI AGTACT 1 cut(s) 3661
SchI GAGTC 6 cut(s) 790, 2485, 2908, 3764, 3901, 5006
ScrFI CCNGG 7 cut(s) 101, 1694, 2309, 3584, 3646, 4232, 4708
SduI GDGCHC 3 cut(s) 597, 1667, 4692
SfcI CTRYAG 7 cut(s) 776, 1603, 1906, 1922, 2165, 2850, 3010
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 6 cut(s) 115, 2389, 3357, 3469, 3775, 4702
SmiI ATTTAAAT 1 cut(s) 921
SmlI CTYRAG 7 cut(s) 125, 614, 1298, 2570, 3335, 4252, 4316
SmoI CTYRAG 7 cut(s) 125, 614, 1298, 2570, 3335, 4252, 4316
SpeI ACTAGT 1 cut(s) 4549
SseBI AGGCCT 1 cut(s) 405
SsiI CCGC 6 cut(s) 13, 977, 2642, 3706, 4693, 5069
SspI AATATT 3 cut(s) 577, 4051, 4573
StuI AGGCCT 1 cut(s) 405
StyD4I CCNGG 7 cut(s) 99, 1692, 2307, 3582, 3644, 4230, 4706
StyI CCWWGG 6 cut(s) 640, 715, 3131, 3975, 4260, 4580
SwaI ATTTAAAT 1 cut(s) 921
TaqII GACCGA 2 cut(s) 2033, 2406
TatI WGTACW 7 cut(s) 2441, 3242, 3659, 3670, 3889, 4464, 4925
TauI GCSGC 2 cut(s) 15, 3709
TfiI GAWTC 9 cut(s) 599, 619, 1135, 2909, 3415, 4134, 4279, 4338, 4556
TscAI CASTG 7 cut(s) 451, 486, 780, 1165, 1972, 4086, 4390
TseFI GTSAC 6 cut(s) 498, 1512, 2045, 2500, 4247, 4754
Tsp45I GTSAC 6 cut(s) 498, 1512, 2045, 2500, 4247, 4754
TspGWI ACGGA 7 cut(s) 891, 1882, 2488, 3342, 4391, 4532, 5045
TspRI CASTG 7 cut(s) 451, 486, 780, 1165, 1972, 4086, 4390
Tth111I GACNNNGTC 2 cut(s) 2695, 5045
Van91I CCANNNNNTGG 5 cut(s) 196, 397, 1352, 1369, 2729
Vha464I CTTAAG 1 cut(s) 1298
VpaK11BI GGWCC 6 cut(s) 115, 2389, 3357, 3469, 3775, 4702
VspI ATTAAT 1 cut(s) 3453
XbaI TCTAGA 2 cut(s) 229, 1402
XceI RCATGY 5 cut(s) 1210, 1447, 2207, 3386, 3563
XcmI CCANNNNNNNNNTGG 1 cut(s) 647
XmaJI CCTAGG 1 cut(s) 715
XmiI GTMKAC 2 cut(s) 2098, 4453
XmnI GAANNNNTTC 2 cut(s) 1032, 3089
ZraI GACGTC 2 cut(s) 1854, 3080
ZrmI AGTACT 1 cut(s) 3661
Zsp2I ATGCAT 1 cut(s) 1569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.