Rh2BG198300

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000307
Physical Location & Seq
Reverse (-)
18199856 .. 18200654
799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG198300.1

Sequence Viewer

Length: 708 bp
ATGCGTCTGTTGTACCAGCAAGAAGAGGGGGAGTTTGAGAGCGCGATAACTGAGTGCTGGACCTCTTTCTTTGATAATCTGTCTTTCATCCGTAGACTTGTAGAAGCAGATGATCAAAATGTTCAACCATGGACAAGGCATGAACTTCCAATGCTTGAAGGGCATATCAAGGTCTTTGATCCGGATATACTTTATAATGCAATTCCCACTTCTACTGAAATTCCGAAGTGGTTTGGGCTTTCCATTAACGGCAAGGACGATTACGCCATGAACTGGTATGAATACCAGTTACCACCATCTTTGAGTGACAAAAACTGGATAGGATTGGCCATCTGTGCATCACATGGTATTAGCAGAGGGTATTTGGTGGAACGTGATTCCTGCCCCTTTATTTTAACATTGAAAACTGCAGACAATGGCTTGTCATCTCTCCATCGGTATCAGATGACCAATGAAGAATCTGAGTTCCTAAAGCGTTGTTGTAATGTGCATGGTGAATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGACGAGTCTGTCCTCATTGTTTCATCTAGTGAGAACAGCTGGAAGCCGCGCTCGGTATATCTCCGTTTTGTGTATGCGCATGAGGTGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGCCTGCCCAACAGTAAAAAGATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

235

Amino Acids

27.58

Weight (kDa)

5.5

Isoelectric Point (pI)

50.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AasI GACNNNNNNGTC 1 cut(s) 557
Acc16I TGCGCA 1 cut(s) 627
AccB7I CCANNNNNTGG 1 cut(s) 273
AccI GTMKAC 1 cut(s) 94
AccII CGCG 2 cut(s) 44, 598
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 1 cut(s) 596
AclWI GGATC 1 cut(s) 173
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 2 cut(s) 219, 497
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 4 cut(s) 125, 158, 403, 661
AluBI AGCT 3 cut(s) 544, 588, 652
AluI AGCT 3 cut(s) 544, 588, 652
AlwI GGATC 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 649
ApoI RAATTY 2 cut(s) 219, 497
AspLEI GCGC 3 cut(s) 44, 600, 628
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 506
AvaII GGWCC 1 cut(s) 60
BalI TGGCCA 1 cut(s) 329
BbvI GCAGC 1 cut(s) 661
BccI CCATC 3 cut(s) 304, 338, 441
BceAI ACGGC 1 cut(s) 265
BclI TGATCA 1 cut(s) 112
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 1 cut(s) 408
BisI GCNGC 2 cut(s) 596, 650
BlsI GCNGC 2 cut(s) 597, 651
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 60
BmsI GCATC 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 3 cut(s) 278, 286, 320
BseAI TCCGGA 1 cut(s) 181
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMII CTCAG 2 cut(s) 42, 453
BseNI ACTGG 3 cut(s) 278, 286, 320
BseXI GCAGC 1 cut(s) 661
Bsh1236I CGCG 2 cut(s) 44, 598
BshFI GGCC 1 cut(s) 329
BsiSI CCGG 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 273
BsnI GGCC 1 cut(s) 329
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 3 cut(s) 112, 178, 698
Bsp19I CCATGG 1 cut(s) 128
BspACI CCGC 1 cut(s) 596
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 2 cut(s) 43, 454
BspEI TCCGGA 1 cut(s) 181
BspFNI CGCG 2 cut(s) 44, 598
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 1 cut(s) 173
BsrI ACTGG 3 cut(s) 278, 286, 320
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 3 cut(s) 112, 178, 698
BssT1I CCWWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 691
Bst6I CTCTTC 2 cut(s) 18, 633
BstC8I GCNNGC 1 cut(s) 682
BstDEI CTNAG 2 cut(s) 51, 462
BstDSI CCRYGG 1 cut(s) 128
BstF5I GGATG 1 cut(s) 87
BstFNI CGCG 2 cut(s) 44, 598
BstHHI GCGC 3 cut(s) 44, 600, 628
BstKTI GATC 3 cut(s) 115, 181, 701
BstMBI GATC 3 cut(s) 112, 178, 698
BstMWI GCNNNNNNNGC 2 cut(s) 160, 335
BstSFI CTRYAG 1 cut(s) 408
BstUI CGCG 2 cut(s) 44, 598
BstV1I GCAGC 1 cut(s) 661
BstXI CCANNNNNNTGG 1 cut(s) 528
BsuRI GGCC 1 cut(s) 329
BtgI CCRYGG 1 cut(s) 128
BtsCI GGATG 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 682
CfoI GCGC 3 cut(s) 44, 600, 628
Cfr13I GGNCC 1 cut(s) 60
CseI GACGC 1 cut(s) 534
Csp6I GTAC 1 cut(s) 13
CspCI CAANNNNNGTGG 2 cut(s) 282, 317
CviAII CATG 6 cut(s) 129, 140, 268, 344, 491, 629
CviJI RGCY 8 cut(s) 238, 329, 420, 508, 544, 588, 595, 652
CviKI_1 RGCY 8 cut(s) 238, 329, 420, 508, 544, 588, 595, 652
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 2 cut(s) 51, 462
DpnI GATC 3 cut(s) 114, 180, 700
DpnII GATC 3 cut(s) 112, 178, 698
DrdI GACNNNNNNGTC 1 cut(s) 557
DseDI GACNNNNNNGTC 1 cut(s) 557
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 18, 633
EarI CTCTTC 2 cut(s) 18, 633
Eco130I CCWWGG 1 cut(s) 128
Eco47I GGWCC 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 6 cut(s) 132, 143, 271, 347, 494, 632
FatI CATG 6 cut(s) 128, 139, 267, 343, 490, 628
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 94
Fnu4HI GCNGC 2 cut(s) 596, 650
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 2 cut(s) 596, 650
FspAI RTGCGCAY 1 cut(s) 627
FspBI CTAG 1 cut(s) 576
FspI TGCGCA 1 cut(s) 627
GlaI GCGC 3 cut(s) 43, 599, 627
GluI GCNGC 2 cut(s) 596, 650
HaeIII GGCC 1 cut(s) 329
HapII CCGG 1 cut(s) 182
HgaI GACGC 1 cut(s) 534
HhaI GCGC 3 cut(s) 44, 600, 628
Hin1II CATG 6 cut(s) 132, 143, 271, 347, 494, 632
Hin6I GCGC 3 cut(s) 42, 598, 626
HinP1I GCGC 3 cut(s) 42, 598, 626
HinfI GANTC 3 cut(s) 377, 458, 554
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 1 cut(s) 95
Hpy188I TCNGA 3 cut(s) 225, 444, 463
Hpy188III TCNNGA 2 cut(s) 182, 702
Hpy8I GTNNAC 1 cut(s) 95
Hpy99I CGWCG 1 cut(s) 528
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 1 cut(s) 691
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 4 cut(s) 200, 338, 410, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 160, 335
HpyF3I CTNAG 2 cut(s) 51, 462
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 6 cut(s) 132, 143, 271, 347, 494, 632
HspAI GCGC 3 cut(s) 42, 598, 626
Kpn2I TCCGGA 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 3 cut(s) 112, 178, 698
Lsp1109I GCAGC 1 cut(s) 661
LweI GCATC 1 cut(s) 347
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 2 cut(s) 288, 305
MalI GATC 3 cut(s) 114, 180, 700
MboI GATC 3 cut(s) 112, 178, 698
MboII GAAGA 4 cut(s) 35, 467, 650, 656
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 3 cut(s) 201, 219, 497
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 1 cut(s) 563
MnlI CCTC 5 cut(s) 19, 73, 350, 572, 625
Mox20I TGGCCA 1 cut(s) 329
MroI TCCGGA 1 cut(s) 181
MscI TGGCCA 1 cut(s) 329
MseI TTAA 3 cut(s) 246, 395, 645
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 588
MspI CCGG 1 cut(s) 182
MvnI CGCG 2 cut(s) 44, 598
MwoI GCNNNNNNNGC 2 cut(s) 160, 335
NcoI CCATGG 1 cut(s) 128
NdeII GATC 3 cut(s) 112, 178, 698
NlaIII CATG 6 cut(s) 132, 143, 271, 347, 494, 632
NmuCI GTSAC 1 cut(s) 305
NsbI TGCGCA 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 2 cut(s) 377, 458
PflMI CCANNNNNTGG 1 cut(s) 273
PkrI GCNGC 2 cut(s) 597, 651
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PsiI TTATAA 1 cut(s) 195
PspPI GGNCC 1 cut(s) 60
PstI CTGCAG 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 588
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
SaqAI TTAA 3 cut(s) 246, 395, 645
SatI GCNGC 2 cut(s) 596, 650
Sau3AI GATC 3 cut(s) 112, 178, 698
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 1 cut(s) 563
SetI ASST 7 cut(s) 65, 174, 376, 546, 590, 636, 654
SfaNI GCATC 1 cut(s) 347
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 60
Sse9I AATT 3 cut(s) 201, 219, 497
SsiI CCGC 1 cut(s) 596
SspMI CTAG 1 cut(s) 576
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 1 cut(s) 691
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 670
TasI AATT 3 cut(s) 201, 219, 497
TauI GCSGC 1 cut(s) 598
TfiI GAWTC 2 cut(s) 377, 458
Tru1I TTAA 3 cut(s) 246, 395, 645
Tru9I TTAA 3 cut(s) 246, 395, 645
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 1 cut(s) 649
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 8 cut(s) 76, 156, 284, 294, 468, 490, 561, 656
TspGWI ACGGA 2 cut(s) 80, 602
Van91I CCANNNNNTGG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 2 cut(s) 219, 497
XmiI GTMKAC 1 cut(s) 94
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.