RLG00000017511

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000307
Physical Location & Seq
Reverse (-)
17700397 .. 17707793
7397 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017511

Sequence Viewer

Length: 4395 bp
ATGGGTACTTTATCAAAACTTTATTTAGATGGCACGGCTATACGGGAGCTGCCTGTATCAATCCAGCATTTGGAAGGCCTTGTTTTGCTAAATCTAAGCGGCTGCAGGAACCTTCGGAGTCTTCCTAGTGAACTTTGTCGTCATTTGACATCACTGAAATTTCTCTATCTGTCATTGTGCTCAAGCATGGAAAAACTGCCAGAAAACATAGACTACTTGAAACACTTGGAGGAGCTTGATGCTTGTGATACTGCTATACGAAAAGTGCCCGATTCCATTTCAGGTCTCATGAATCTAAAACTATTGTGTTTCCATGGATGTTCTGGATTCACAGGTTTGAAGTTGCCAAACAAGTTCTCAGGTTTAACATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCACAGAAGGAGCAATCCCCAATGACATTGGTGATTTATTCTCACTGCAGAGTCTGGATTTAAGATGTTCAAAGCTAAAAGAGATTCCAGATATTATGGGAGATATGGATAATTTTTCACAACTGCATTTAGATGGGACGGCGTTAAAGCATCTGTTAATGCCGCCGCAGCATTTGAAATGCCCTATTTCGATAAATCTAAGAGGCTGCAAGAACCCTTCGACTGGCTGCTCACGTATATCCAAATTGCCATCTGGCCTGGAAAGCTTGGAACATTTGGAGGAGCTTGATGTTTCTGAAACTGCTATAACAGTAGTATCCAAGTCCTTTACATTTTTGGAGAAGCTTAAAGTTGTTATCTTTCGGTGGTTTGCGGATTTAAGCTTTTTGACATCATTAAATCTACGGAGGTGTGGTCTAGCAGAAGAAGTCGTTGGTATCCTGTGCTGCTTATCGTCTCTGAAGATTCTGGATTTGAGTGAGAACAGGTTTGTGACTATACCTAATGACATTGGTCGCTTATCCTCTTTGCATCGATTGGATTTGAGTGGAAACAATATAGTGAGCATACCGCATGAAATTGGGCACTTATCCTCCTTGCAGCAATTGGATTTGAGTGGAAACAATATAGTGAGCATACCGCATGAAATTGGGCACTTATCCTCTTTGCAGCAATTGGATATGAGTGGAAACAATATAGTAAGCATACCACATGAAATTGGTCGCTTATCCTCTTTGCAGCTTTTAGATTTGAGTGAAAACAACTTTGTGAGTATACCTAAGAGCATCTCTAATCTTTCTGGGCTTACAGAACTTCGCCTGTTGAGATGTAGTAAGCTACAGTCCTTGCCAAATGATCTTCCCTTCAATCTAAAACATGTTTATGCACAAGAATGTCCTATGTTGAAGTATAATGCAGATACTTTGACAATACGAGCATCTGGAGAAGGGTTCTGTTTCATGCATTGCGGAAAATATGAGCAGGATGATGATCGGCCAAGCCACGTCCTAGTTCCAGTTCCCAAAGACCTCATTGAACTACACATGTATTTTGGAAAATATGAGCAGTATGATGATCAGCCAAGCCACGTCCTAGTTCCAGTTCCCAAAGACCGCATTGAACTATACATTGCGGACCGAGTTTATGGTAAAAAGCCATTTGAGATTCGTTTTCCACACAGTACAAGAATTCCGAATCTATGGACTCATTGGAGGAGTGGCCCTTCTGTAACAATCCCATTATCTGATGGTAACAGTGCGTGTATGGGACTTGCTCTGTTTGTTGTTTTTGAAATCCTTGAGAAAGGCAATTTCAACAAGATCTGGGAATCGGAGGACACTATCTGTGAATTTCAGATTGATGTTGGGCCTGAAAATTCCCTAGTTTTTGAGAACTTCATAGACTTCATGGCCGGGTCATATGCACTTTGTTGCTATGAACCGCAAGGCGGGCAATTGAGTGGGATATTCGATAAACCAAGCTCACAACTTCGAGCTTCAGTTTCAACGAAGAGACTGAATTTAAAAGTGAGAGGCTGTGGGATACATCTAATATCAGAAGAAAATGCTGCAGAGTTTGTAAAAAGTTTAGCCAATCAGACTCTTATTTCCAGTTTTGATCGACATTGCGAGTACATATTAAATGAGGAGACAATAACTGCTGACCTGAAGGAACTGGAATCTACTTCAACTTTCAATGAAGATAGCTGCAGCAAAACTAACTCTAAAATCAAACTCAGAGGACAGTTGTCTATACTCTATGAGTTTCATGATGCAGGGGATGTAACACTTTGTCACATTACTGAAAACTTACTTGATGATCAGAAATGGGTGGGATTAGAGCTTTATGTTAGATTTACTTTGCGTGCATCTGCTTTTAGACATGGCAATAACAGTTTCTTGACTTTCTTCTTTAACGTTGATTTCTGTGCTTATGATCACGAAAGTATAGTAATGCATCAATCCCTCGAGATTAATAGTTGTCTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCATGCATTCATTTTCAACAACACTTGAATCAACGTAAGGGTATTAGCGCATTGTTCAGAACCATTAACCCTGAAATGGAGGTCCAAGTGTGTGGGAGCCGTTTAGCCTTCGAGCATGATCTTGAAGACTTGACCAAATCATTAACTGAGGTTGCAGGCATGTTTGAGAAATATGTCCCCACTCATATTTGCTCCCTAGGCCAACCTGTTGATCGGCGCAATGCAGTGGAAGCAGAAATGCCCATCAATTCCGGTTCCTGGTTCCGAAGAAGGGCTGCACTAGTACTACCAAAACAAGCTCCTGCTCCTTCTTCCACAATAGTGCGCCTGCAAAATCACAGATGCTATATCCAGCAACAGCAAGGTCTTGTGGAGTCTGACCTTACCGAATTGGTCTACATGAGATCAGTAATGTTACTTCACTCTAGAGGTCTTCTTTTCGAAAACAAGGAGAGAAACCAAGAAATTGTTGCAGAAGAAGATAGAACATTGATGTTGCCTCGTCATCATGTACGTATAATGGCTGAGACTCAACTTCACGAAAGCTATAGTTCAGAACAGTGGAAGAGATGCCTCGAGTTGTTACTTCAACAGTCCAAGGTGGCTACTCTCAGTCTTGGTGGACATACAATTTCCGCTTTCAAGAATTTCGACCCGTCCTCCGCATACAATATTATTTGTCCCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCTCATCGGATGTCTTCTAGTTCCAGGGTGGGAATCAGACTACCACTGAATTTGCATGATGTTGACAGTTGGCGGGGACTTGCTATCTGTGTTGCCTTTGAAGTTAATGACCAGGGTGCAATGGCCTCCCTGGTCAATCTTCTCTGTCACTTGAGAGCCAATGATGGCGACTATTGCTTGAATCCTATGCCCATGCGTTCCATCACTAGTAAGAAACTCAAGCCGTTGCATATTCGCAGATTCATTTGGCTGACCTATATACCCCGTGTTCTTCTAACAGAGTTAAATGTGGTTAATGATGTTGAGGCTAGAATTTATGTTAGTTCCCCTGGCTTGACAGTGGACAGTTGTGGTATCCGTCTCTTGTATGGGCATGAAGAGAGGGAATTTAACAGTGTAGTAACGGAGTGCTGGACCTCCTTTTTTGATAATTTGCCTCTCACTCGTCAACTGGTAGATGGTCAAAATGTCCAACCATGGGTAAGGCATGAACTACCGATGCGTGAAGGGCATGTCAAGGGACTGAGCCATGCCGGTGAACCTACCGATGAGCTATCCGGTCAGTTCGAGCTCCAGCTCCAGCTGGAGCCACAAGGCATCCGACTCTCCAATACTACGCACTCCGAAGTCGCTCCTTGCTTGCCTCTGCCTTCCCTAAATATGCTCTGCGGCGCCTCCGATCAGGAACTCTGGTTGTTCGATGAGCCGAGTCGGAGCAGTTCGATGAGCCGAGTCAGAGTAGGATTGCTGATCTGCTTCGTGCTACTGACGAAGCTGAGGGAGGATACTACGGCAGTTCAATATGGCTACGTCCAACCTATGAATCTTCACGATGAAGTTTTACAGTACAATCCAGAAGCCTTTCAGTACACCACTCCAGGATTGACTATCTGTGCACCACATGGTATTAGCAAAAGGTATTTGGTGGAACATCGTTCCTACCCCTTTATTTTAATATTGGAAACTGGAAAGAATGGCTTGTCATCTATCCATGATCAGTATGAGATGACCAATGAAGAAACTGAGTTCCTAAAGCGTTGTTGTAATGTGCATGGTGAATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGACGAGTCTGTCCTCATTGTTTCATTTGGTGAGCACTGCTGGAAGCCGCGCGCGATATATCTCCGTTTTGTGTATGCGCCTGAGGTGGAAGAGTTTATGCAGCTCTGTTTCAATCTTCATCGACCCCCTGCCCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8

Protein Analysis

1465

Amino Acids

165.76

Weight (kDa)

5.87

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 30 - 130 1.8e-12 Leucine-rich repeat region
LRR_14 PF23598 262 - 362 7.9e-14 Leucine-rich repeat region
LRR_8 PF13855 285 - 321 6.2e-06 Leucine rich repeat
LRR_8 PF13855 310 - 367 2.1e-12 Leucine rich repeat
LRR_4 PF12799 310 - 346 1.2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 327 - 412 9.6e-14 Leucine-rich repeat region
LRR_4 PF12799 333 - 369 7.3e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 356 - 408 1.1e-06 Leucine rich repeat
C-JID PF20160 1038 - 1174 2.1e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 4262
AccB1I GGYRCC 1 cut(s) 3815
AccB7I CCANNNNNTGG 1 cut(s) 70
AccI GTMKAC 2 cut(s) 1177, 2817
AccII CGCG 3 cut(s) 4303, 4305, 4307
AclI AACGTT 1 cut(s) 2319
AcoI YGGCCR 2 cut(s) 1397, 1812
AcuI CTGAAG 3 cut(s) 884, 1884, 2090
AcyI GRCGYC 1 cut(s) 3816
AfaI GTAC 8 cut(s) 7, 1585, 2036, 2421, 2706, 2934, 3992, 4013
AfiI CCNNNNNNNGG 7 cut(s) 70, 626, 1850, 2497, 2679, 2692, 3622
AflIII ACRYGT 3 cut(s) 1279, 1446, 2416
AhlI ACTAGT 2 cut(s) 2701, 3350
AjiI CACGTC 2 cut(s) 1408, 1492
AjnI CCWGG 7 cut(s) 660, 2678, 3167, 3255, 3273, 3472, 4021
AjuI GAANNNNNNNTTGG 2 cut(s) 819, 851
AleI CACNNNNGTG 1 cut(s) 2741
Alw21I GWGCWC 4 cut(s) 182, 3717, 4042, 4290
Alw26I GTCTC 7 cut(s) 290, 864, 1909, 2045, 2942, 3150, 3509
Alw44I GTGCAC 1 cut(s) 4038
AlwNI CAGNNNCTG 1 cut(s) 457
Ama87I CYCGRG 2 cut(s) 2369, 2996
AoxI GGCC 8 cut(s) 76, 658, 1397, 1621, 1769, 1812, 2620, 3267
ApaLI GTGCAC 1 cut(s) 4038
AseI ATTAAT 1 cut(s) 2376
Asp700I GAANNNNTTC 1 cut(s) 4005
AspA2I CCTAGG 2 cut(s) 388, 2617
AspLEI GCGC 7 cut(s) 2471, 2640, 2748, 3818, 4305, 4307, 4333
AspS9I GGNCC 6 cut(s) 1537, 1622, 1769, 2391, 2503, 3558
AsuC2I CCSGG 1 cut(s) 1816
AsuHPI GGTGA 4 cut(s) 446, 3692, 4211, 4295
AsuII TTCGAA 1 cut(s) 2862
AvaI CYCGRG 2 cut(s) 2369, 2996
AvaII GGWCC 4 cut(s) 1537, 2391, 2503, 3558
AvrII CCTAGG 2 cut(s) 388, 2617
AxyI CCTNAGG 1 cut(s) 4335
BaeGI GKGCMC 4 cut(s) 270, 990, 1059, 4042
BaeI ACNNNNGTAYC 2 cut(s) 702, 735
BanI GGYRCC 1 cut(s) 3815
BanII GRGCYC 1 cut(s) 3717
BarI GAAGNNNNNNTAC 2 cut(s) 2823, 2855
BbsI GAAGAC 4 cut(s) 113, 2553, 2846, 3150
Bbv12I GWGCWC 4 cut(s) 182, 3717, 4042, 4290
BbvCI CCTCAGC 1 cut(s) 3920
BccI CCATC 8 cut(s) 23, 530, 661, 1643, 2672, 3302, 3353, 3596
BceAI ACGGC 5 cut(s) 51, 558, 2505, 3352, 3951
BciT130I CCWGG 7 cut(s) 662, 2680, 3169, 3257, 3275, 3474, 4023
BciVI GTATCC 5 cut(s) 730, 851, 1938, 3509, 3922
BclI TGATCA 4 cut(s) 1477, 2221, 2338, 4138
BcnI CCSGG 1 cut(s) 1816
BcoDI GTCTC 7 cut(s) 290, 864, 1909, 2045, 2942, 3150, 3509
BcuI ACTAGT 2 cut(s) 2701, 3350
BfmI CTRYAG 6 cut(s) 103, 449, 1241, 1971, 2110, 2968
BfoI RGCGCY 1 cut(s) 3819
BfuI GTATCC 5 cut(s) 730, 851, 1938, 3509, 3922
BglII AGATCT 1 cut(s) 1722
BlnI CCTAGG 2 cut(s) 388, 2617
BmcAI AGTACT 1 cut(s) 2706
Bme1390I CCNGG 8 cut(s) 662, 1816, 2680, 3169, 3257, 3275, 3474, 4023
Bme18I GGWCC 4 cut(s) 1537, 2391, 2503, 3558
BmeT110I CYCGRG 2 cut(s) 2369, 2996
BmgBI CACGTC 2 cut(s) 1408, 1492
BmgT120I GGNCC 6 cut(s) 1537, 1622, 1769, 2391, 2503, 3558
BmiI GGNNCC 7 cut(s) 110, 2392, 2519, 2677, 2684, 3732, 3817
BmrFI CCNGG 8 cut(s) 662, 1816, 2680, 3169, 3257, 3275, 3474, 4023
BoxI GACNNNNGTC 2 cut(s) 915, 2149
BpiI GAAGAC 4 cut(s) 113, 2553, 2846, 3150
BpmI CTGGAG 5 cut(s) 1365, 3701, 3707, 3749, 4005
Bpu10I CCTNAGC 1 cut(s) 3920
Bpu14I TTCGAA 1 cut(s) 2862
BpuEI CTTGAG 4 cut(s) 166, 1721, 3316, 3347
BpuMI CCSGG 1 cut(s) 1816
Bsa29I ATCGAT 1 cut(s) 937
BsaAI YACGTR 2 cut(s) 638, 2936
BsaBI GATNNNNATC 1 cut(s) 1392
BsaHI GRCGYC 1 cut(s) 3816
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 9 cut(s) 313, 388, 2617, 3018, 3168, 3256, 3273, 3472, 3620
BsaWI WCCGGW 2 cut(s) 2672, 3701
BsaXI ACNNNNNCTCC 6 cut(s) 802, 832, 980, 1010, 3065, 3095
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 626, 1850, 2497, 2679, 2692, 3622
Bse118I RCCGGY 1 cut(s) 3677
Bse1I ACTGG 8 cut(s) 631, 1418, 1502, 2013, 2082, 3122, 3600, 4114
Bse21I CCTNAGG 1 cut(s) 4335
Bse3DI GCAATG 5 cut(s) 1366, 1530, 2026, 2647, 3270
Bse8I GATNNNNATC 1 cut(s) 1392
BseBI CCWGG 7 cut(s) 662, 2680, 3169, 3257, 3275, 3474, 4023
BseCI ATCGAT 1 cut(s) 937
BseDI CCNNGG 9 cut(s) 313, 388, 2617, 3018, 3168, 3256, 3273, 3472, 3620
BseGI GGATG 6 cut(s) 323, 401, 1393, 2188, 3159, 3741
BseJI GATNNNNATC 1 cut(s) 1392
BseLI CCNNNNNNNGG 7 cut(s) 70, 626, 1850, 2497, 2679, 2692, 3622
BseMI GCAATG 5 cut(s) 1366, 1530, 2026, 2647, 3270
BseNI ACTGG 8 cut(s) 631, 1418, 1502, 2013, 2082, 3122, 3600, 4114
BsePI GCGCGC 1 cut(s) 4303
BseRI GAGGAG 4 cut(s) 245, 698, 1630, 2063
BseSI GKGCMC 4 cut(s) 270, 990, 1059, 4042
BsgI GTGCAG 1 cut(s) 2682
Bsh1236I CGCG 3 cut(s) 4303, 4305, 4307
BshFI GGCC 8 cut(s) 78, 660, 1399, 1623, 1771, 1814, 2622, 3269
BshNI GGYRCC 1 cut(s) 3815
BshVI ATCGAT 1 cut(s) 937
BsiHKAI GWGCWC 4 cut(s) 182, 3717, 4042, 4290
BsiHKCI CYCGRG 2 cut(s) 2369, 2996
BsiSI CCGG 4 cut(s) 1815, 2673, 3678, 3702
BslFI GGGAC 7 cut(s) 553, 1683, 2404, 2582, 3087, 3234, 3678
BslI CCNNNNNNNGG 7 cut(s) 70, 626, 1850, 2497, 2679, 2692, 3622
BsmAI GTCTC 7 cut(s) 290, 864, 1909, 2045, 2942, 3150, 3509
BsmBI CGTCTC 2 cut(s) 864, 3509
BsmFI GGGAC 7 cut(s) 553, 1683, 2404, 2582, 3087, 3234, 3678
BsmI GAATGC 1 cut(s) 2427
BsnI GGCC 8 cut(s) 78, 660, 1399, 1623, 1771, 1814, 2622, 3269
Bso31I GGTCTC 1 cut(s) 290
BsoBI CYCGRG 2 cut(s) 2369, 2996
Bsp119I TTCGAA 1 cut(s) 2862
Bsp1286I GDGCHC 7 cut(s) 182, 270, 990, 1059, 3717, 4042, 4290
Bsp19I CCATGG 2 cut(s) 313, 3620
BspANI GGCC 8 cut(s) 78, 660, 1399, 1623, 1771, 1814, 2622, 3269
BspDI ATCGAT 1 cut(s) 937
BspFNI CGCG 3 cut(s) 4303, 4305, 4307
BspHI TCATGA 2 cut(s) 288, 2170
BspLI GGNNCC 7 cut(s) 110, 2392, 2519, 2677, 2684, 3732, 3817
BspMAI CTGCAG 4 cut(s) 107, 453, 1975, 2114
BspT104I TTCGAA 1 cut(s) 2862
BspT107I GGYRCC 1 cut(s) 3815
BspTNI GGTCTC 1 cut(s) 290
BsrDI GCAATG 5 cut(s) 1366, 1530, 2026, 2647, 3270
BsrFI RCCGGY 1 cut(s) 3677
BsrI ACTGG 8 cut(s) 631, 1418, 1502, 2013, 2082, 3122, 3600, 4114
BssAI RCCGGY 1 cut(s) 3677
BssECI CCNNGG 9 cut(s) 313, 388, 2617, 3018, 3168, 3256, 3273, 3472, 3620
BssHII GCGCGC 1 cut(s) 4303
BssNAI GTATAC 1 cut(s) 1178
BssNI GRCGYC 1 cut(s) 3816
BssT1I CCWWGG 5 cut(s) 313, 388, 2617, 3018, 3620
Bst1107I GTATAC 1 cut(s) 1178
Bst2UI CCWGG 7 cut(s) 662, 2680, 3169, 3257, 3275, 3474, 4023
Bst6I CTCTTC 4 cut(s) 1907, 2981, 3516, 4338
BstACI GRCGYC 1 cut(s) 3816
BstBAI YACGTR 2 cut(s) 638, 2936
BstBI TTCGAA 1 cut(s) 2862
BstC8I GCNNGC 6 cut(s) 1853, 2268, 2578, 2750, 3785, 4305
BstDSI CCRYGG 2 cut(s) 313, 3620
BstF5I GGATG 6 cut(s) 323, 401, 1393, 2188, 3159, 3741
BstFNI CGCG 3 cut(s) 4303, 4305, 4307
BstH2I RGCGCY 1 cut(s) 3819
BstHHI GCGC 7 cut(s) 2471, 2640, 2748, 3818, 4305, 4307, 4333
BstMAI GTCTC 7 cut(s) 290, 864, 1909, 2045, 2942, 3150, 3509
BstMWI GCNNNNNNNGC 7 cut(s) 571, 666, 1852, 2619, 2651, 3318, 3652
BstNI CCWGG 7 cut(s) 662, 2680, 3169, 3257, 3275, 3474, 4023
BstNSI RCATGY 5 cut(s) 1283, 1450, 2420, 2584, 3659
BstPAI GACNNNNGTC 2 cut(s) 915, 2149
BstSCI CCNGG 8 cut(s) 660, 1814, 2678, 3167, 3255, 3273, 3472, 4021
BstSFI CTRYAG 6 cut(s) 103, 449, 1241, 1971, 2110, 2968
BstSLI GKGCMC 4 cut(s) 270, 990, 1059, 4042
BstSNI TACGTA 1 cut(s) 2936
BstUI CGCG 3 cut(s) 4303, 4305, 4307
BstV2I GAAGAC 4 cut(s) 113, 2553, 2846, 3150
BstX2I RGATCY 1 cut(s) 1722
BstXI CCANNNNNNTGG 2 cut(s) 2513, 4233
BstYI RGATCY 1 cut(s) 1722
BstZ17I GTATAC 1 cut(s) 1178
Bsu15I ATCGAT 1 cut(s) 937
Bsu36I CCTNAGG 1 cut(s) 4335
BsuI GTATCC 5 cut(s) 730, 851, 1938, 3509, 3922
BsuRI GGCC 8 cut(s) 78, 660, 1399, 1623, 1771, 1814, 2622, 3269
BsuTUI ATCGAT 1 cut(s) 937
BtgI CCRYGG 2 cut(s) 313, 3620
BtrI CACGTC 2 cut(s) 1408, 1492
BtsCI GGATG 6 cut(s) 323, 401, 1393, 2188, 3159, 3741
BtsI GCAGTG 3 cut(s) 446, 2652, 4288
Cac8I GCNNGC 6 cut(s) 1853, 2268, 2578, 2750, 3785, 4305
CaiI CAGNNNCTG 1 cut(s) 457
CciI TCATGA 2 cut(s) 288, 2170
CfoI GCGC 7 cut(s) 2471, 2640, 2748, 3818, 4305, 4307, 4333
Cfr10I RCCGGY 1 cut(s) 3677
Cfr13I GGNCC 6 cut(s) 1537, 1622, 1769, 2391, 2503, 3558
ClaI ATCGAT 1 cut(s) 937
CpoI CGGWCCG 1 cut(s) 1537
CseI GACGC 1 cut(s) 4239
Csp6I GTAC 8 cut(s) 6, 1584, 2035, 2420, 2705, 2933, 3991, 4012
CspCI CAANNNNNGTGG 2 cut(s) 3177, 3212
CspI CGGWCCG 1 cut(s) 1537
CviQI GTAC 8 cut(s) 6, 1584, 2035, 2420, 2705, 2933, 3991, 4012
DinI GGCGCC 1 cut(s) 3817
DraI TTTAAA 1 cut(s) 1926
DrdI GACNNNNNNGTC 1 cut(s) 4262
DseDI GACNNNNNNGTC 1 cut(s) 4262
EaeI YGGCCR 2 cut(s) 1397, 1812
Eam1104I CTCTTC 4 cut(s) 1907, 2981, 3516, 4338
EarI CTCTTC 4 cut(s) 1907, 2981, 3516, 4338
Ecl136II GAGCTC 1 cut(s) 3715
Eco105I TACGTA 1 cut(s) 2936
Eco130I CCWWGG 5 cut(s) 313, 388, 2617, 3018, 3620
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 3717
Eco31I GGTCTC 1 cut(s) 290
Eco47I GGWCC 4 cut(s) 1537, 2391, 2503, 3558
Eco53kI GAGCTC 1 cut(s) 3715
Eco57I CTGAAG 3 cut(s) 884, 1884, 2090
Eco81I CCTNAGG 1 cut(s) 4335
Eco88I CYCGRG 2 cut(s) 2369, 2996
EcoICRI GAGCTC 1 cut(s) 3715
EcoO109I RGGNCCY 1 cut(s) 2391
EcoRI GAATTC 2 cut(s) 1590, 4202
EcoRII CCWGG 7 cut(s) 660, 2678, 3167, 3255, 3273, 3472, 4021
EcoT14I CCWWGG 5 cut(s) 313, 388, 2617, 3018, 3620
EcoT22I ATGCAT 3 cut(s) 1368, 2361, 2429
EcoT38I GRGCYC 1 cut(s) 3717
EgeI GGCGCC 1 cut(s) 3817
EheI GGCGCC 1 cut(s) 3817
ErhI CCWWGG 5 cut(s) 313, 388, 2617, 3018, 3620
Esp3I CGTCTC 2 cut(s) 864, 3509
FalI AAGNNNNNCTT 2 cut(s) 4106, 4138
FaqI GGGAC 7 cut(s) 553, 1683, 2404, 2582, 3087, 3234, 3678
FauI CCCGC 2 cut(s) 1844, 3210
FauNDI CATATG 1 cut(s) 1822
FbaI TGATCA 4 cut(s) 1477, 2221, 2338, 4138
FblI GTMKAC 2 cut(s) 1177, 2817
FokI GGATG 6 cut(s) 330, 408, 1400, 2195, 3166, 3728
FriOI GRGCYC 1 cut(s) 3717
GlaI GCGC 7 cut(s) 2470, 2639, 2747, 3817, 4304, 4306, 4332
GsuI CTGGAG 5 cut(s) 1365, 3701, 3707, 3749, 4005
HaeII RGCGCY 1 cut(s) 3819
HaeIII GGCC 8 cut(s) 78, 660, 1399, 1623, 1771, 1814, 2622, 3269
HapII CCGG 4 cut(s) 1815, 2673, 3678, 3702
HgaI GACGC 1 cut(s) 4239
HhaI GCGC 7 cut(s) 2471, 2640, 2748, 3818, 4305, 4307, 4333
Hin1I GRCGYC 1 cut(s) 3816
Hin6I GCGC 7 cut(s) 2469, 2638, 2746, 3816, 4303, 4305, 4331
HinP1I GCGC 7 cut(s) 2469, 2638, 2746, 3816, 4303, 4305, 4331
HincII GTYRAC 2 cut(s) 3208, 3593
HindII GTYRAC 2 cut(s) 3208, 3593
HindIII AAGCTT 3 cut(s) 667, 746, 784
HpaII CCGG 4 cut(s) 1815, 2673, 3678, 3702
HphI GGTGA 4 cut(s) 446, 3692, 4211, 4295
Hpy99I CGWCG 1 cut(s) 4233
HpyCH4IV ACGT 7 cut(s) 637, 1407, 1491, 2319, 2455, 2935, 3954
HpyF10VI GCNNNNNNNGC 7 cut(s) 571, 666, 1852, 2619, 2651, 3318, 3652
HpySE526I ACGT 7 cut(s) 637, 1407, 1491, 2319, 2455, 2935, 3954
Hsp92I GRCGYC 1 cut(s) 3816
HspAI GCGC 7 cut(s) 2469, 2638, 2746, 3816, 4303, 4305, 4331
KasI GGCGCC 1 cut(s) 3815
Ksp22I TGATCA 4 cut(s) 1477, 2221, 2338, 4138
MaeII ACGT 7 cut(s) 637, 1407, 1491, 2319, 2455, 2935, 3954
MaeIII GTNAC 9 cut(s) 895, 1630, 1652, 2185, 2195, 2835, 3003, 3290, 3544
MauBI CGCGCGCG 1 cut(s) 4303
MfeI CAATTG 3 cut(s) 1007, 1076, 1856
MflI RGATCY 1 cut(s) 1722
MhlI GDGCHC 7 cut(s) 182, 270, 990, 1059, 3717, 4042, 4290
Mly113I GGCGCC 1 cut(s) 3816
MmeI TCCRAC 4 cut(s) 3640, 3769, 3836, 3982
Mph1103I ATGCAT 3 cut(s) 1368, 2361, 2429
MroXI GAANNNNTTC 1 cut(s) 4005
MslI CAYNNNNRTG 5 cut(s) 534, 1284, 1294, 2741, 3678
MspA1I CMGCKG 1 cut(s) 3727
MspI CCGG 4 cut(s) 1815, 2673, 3678, 3702
MspR9I CCNGG 8 cut(s) 662, 1816, 2680, 3169, 3257, 3275, 3474, 4023
MunI CAATTG 3 cut(s) 1007, 1076, 1856
Mva1269I GAATGC 1 cut(s) 2427
MvaI CCWGG 7 cut(s) 662, 2680, 3169, 3257, 3275, 3474, 4023
MvnI CGCG 3 cut(s) 4303, 4305, 4307
MwoI GCNNNNNNNGC 7 cut(s) 571, 666, 1852, 2619, 2651, 3318, 3652
NarI GGCGCC 1 cut(s) 3816
NciI CCSGG 1 cut(s) 1816
NcoI CCATGG 2 cut(s) 313, 3620
NdeI CATATG 1 cut(s) 1822
NlaIV GGNNCC 7 cut(s) 110, 2392, 2519, 2677, 2684, 3732, 3817
NmeAIII GCCGAG 2 cut(s) 3876, 3899
NmuCI GTSAC 3 cut(s) 895, 2195, 3290
NsiI ATGCAT 3 cut(s) 1368, 2361, 2429
NspI RCATGY 5 cut(s) 1283, 1450, 2420, 2584, 3659
NspV TTCGAA 1 cut(s) 2862
OliI CACNNNNGTG 1 cut(s) 2741
PaeR7I CTCGAG 2 cut(s) 2369, 2996
PagI TCATGA 2 cut(s) 288, 2170
PauI GCGCGC 1 cut(s) 4303
PceI AGGCCT 1 cut(s) 78
PciI ACATGT 3 cut(s) 1279, 1446, 2416
PctI GAATGC 1 cut(s) 2427
PdmI GAANNNNTTC 1 cut(s) 4005
PflMI CCANNNNNTGG 1 cut(s) 70
PfoI TCCNGGA 1 cut(s) 4021
PluTI GGCGCC 1 cut(s) 3819
Ppu21I YACGTR 2 cut(s) 638, 2936
PpuMI RGGWCCY 1 cut(s) 2391
PscI ACATGT 3 cut(s) 1279, 1446, 2416
PshAI GACNNNNGTC 2 cut(s) 915, 2149
PshBI ATTAAT 1 cut(s) 2376
Psp124BI GAGCTC 1 cut(s) 3717
Psp1406I AACGTT 1 cut(s) 2319
Psp5II RGGWCCY 1 cut(s) 2391
Psp6I CCWGG 7 cut(s) 660, 2678, 3167, 3255, 3273, 3472, 4021
PspGI CCWGG 7 cut(s) 660, 2678, 3167, 3255, 3273, 3472, 4021
PspN4I GGNNCC 7 cut(s) 110, 2392, 2519, 2677, 2684, 3732, 3817
PspPI GGNCC 6 cut(s) 1537, 1622, 1769, 2391, 2503, 3558
PspPPI RGGWCCY 1 cut(s) 2391
PspXI VCTCGAGB 1 cut(s) 2996
PstI CTGCAG 4 cut(s) 107, 453, 1975, 2114
PstNI CAGNNNCTG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 1722
PteI GCGCGC 1 cut(s) 4303
PvuII CAGCTG 1 cut(s) 3727
RsaI GTAC 8 cut(s) 7, 1585, 2036, 2421, 2706, 2934, 3992, 4013
RsaNI GTAC 8 cut(s) 6, 1584, 2035, 2420, 2705, 2933, 3991, 4012
RseI CAYNNNNRTG 5 cut(s) 534, 1284, 1294, 2741, 3678
Rsr2I CGGWCCG 1 cut(s) 1537
RsrII CGGWCCG 1 cut(s) 1537
SacI GAGCTC 1 cut(s) 3717
Sau96I GGNCC 6 cut(s) 1537, 1622, 1769, 2391, 2503, 3558
ScaI AGTACT 1 cut(s) 2706
ScrFI CCNGG 8 cut(s) 662, 1816, 2680, 3169, 3257, 3275, 3474, 4023
SduI GDGCHC 7 cut(s) 182, 270, 990, 1059, 3717, 4042, 4290
SfcI CTRYAG 6 cut(s) 103, 449, 1241, 1971, 2110, 2968
SfoI GGCGCC 1 cut(s) 3817
Sfr274I CTCGAG 2 cut(s) 2369, 2996
SfuI TTCGAA 1 cut(s) 2862
SinI GGWCC 4 cut(s) 1537, 2391, 2503, 3558
SlaI CTCGAG 2 cut(s) 2369, 2996
SmiMI CAYNNNNRTG 5 cut(s) 534, 1284, 1294, 2741, 3678
SmlI CTYRAG 6 cut(s) 181, 1700, 2369, 2996, 3295, 3362
SmoI CTYRAG 6 cut(s) 181, 1700, 2369, 2996, 3295, 3362
SnaBI TACGTA 1 cut(s) 2936
SpeI ACTAGT 2 cut(s) 2701, 3350
SseBI AGGCCT 1 cut(s) 78
SspDI GGCGCC 1 cut(s) 3815
SspI AATATT 2 cut(s) 3094, 4101
SstI GAGCTC 1 cut(s) 3717
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 8 cut(s) 660, 1814, 2678, 3167, 3255, 3273, 3472, 4021
StyI CCWWGG 5 cut(s) 313, 388, 2617, 3018, 3620
TaiI ACGT 7 cut(s) 640, 1410, 1494, 2322, 2458, 2938, 3957
TaqII GACCGA 1 cut(s) 1554
TatI WGTACW 5 cut(s) 1583, 2034, 2704, 3990, 4011
TauI GCSGC 5 cut(s) 102, 568, 571, 3816, 4303
TseFI GTSAC 3 cut(s) 895, 2195, 3290
Tsp45I GTSAC 3 cut(s) 895, 2195, 3290
TspGWI ACGGA 4 cut(s) 823, 3491, 3563, 4307
Van91I CCANNNNNTGG 1 cut(s) 70
VneI GTGCAC 1 cut(s) 4038
VpaK11BI GGWCC 4 cut(s) 1537, 2391, 2503, 3558
VspI ATTAAT 1 cut(s) 2376
XbaI TCTAGA 1 cut(s) 2846
XceI RCATGY 5 cut(s) 1283, 1450, 2420, 2584, 3659
XcmI CCANNNNNNNNNTGG 1 cut(s) 320
XhoI CTCGAG 2 cut(s) 2369, 2996
XmaJI CCTAGG 2 cut(s) 388, 2617
XmiI GTMKAC 2 cut(s) 1177, 2817
XmnI GAANNNNTTC 1 cut(s) 4005
ZrmI AGTACT 1 cut(s) 2706
Zsp2I ATGCAT 3 cut(s) 1368, 2361, 2429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.