RLG00000018809

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000307
Physical Location & Seq
Forward (+)
36620722 .. 36650003
29282 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018809

Sequence Viewer

Length: 4398 bp
ATGCAAGAAACTGAGGGGACTGGAAGTCAACGTAGCAGGTTGTGGCTCGATGACAATGTACACAAGTACGAAGGCAGAAGGTCATGGCGTTTTGAGGATGCTGGTAGCGTGCTCATGGACAATACGCTTTCTCTGAGAATGCTTATACTCATGGATCTTTCTGACTGCAAGTATTTAACCAAGACCCTGGACTTCAGTAAGGTCCCAAAGCTTGAGAAATTGATCCTGGAAGGTTGTATAGAGTTATTGGAGGTTCACCCTACAATTGGGGATCTCCAACATTTGGTTTTATTGAACTTGAAAGGTTGTGAAAGTCTAGAGAACCTTCCTCAATCCATCAGACACTTTCCAGAGATTGTGGGGAACATGGATACTTTATCAGAAATTTATTTAGATGACACGGCTATACGGGAGCTGCCTGTATCAATCCAGCATTTGGAAGGCCTCATTTTACTTAATCTAAGTGGGTGCAGGAACCTTCTCAGTCTTCCAGGCATCCTTTCTAGTAGTTTGACATCACTGAAATTTCTCTATCTGTCACTATGCTCACGTATGGACAGACTGCCGGATAACATAGGCTGCTTGGAACACTTGGAGGAGCTTGATGCTTGTAATACTGCTATAAGAAACGTGCCCGATTCCATTTTTCTCCTCAAGAATCTTAAATTATTGTGTTTCCATGGCTGTGCTGGATCCACAGGTTTAGAGTTGCCAAGCAAATTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGAGCAATCCCCAATGACATTGGTGATTTATTCTCACTGCAGAGTATTTATCTAATTACTCTCTTTACGCACATGCAGTTTCGTTTGGGAAATCTGGAAGAACTCGATCTTTCCAACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAATTGAACATTGAAGGTTGTGAAAAATTATCAGAGGTTCACCCAACCATTGGGTGTCTCCAACATTTGGTGTTTCTGAATTTGAAAGGATGTGTAAATCTAGAGAGCCTTCCCAGTTCCATCAGCTTGAAGTCTCTAAACATGCTTGTTCTTTCGGGATGTTCAAAGCTGAAAGAGTTTCCAAACATTAAGGGAGAAATGAACAATTTGTCACAACTGCATTTAGATGGGACTGCGTTAAGGCATCTGCGAATTCCGATGATGCATTTGAAAGGCCCAATTTTGATAAATTTGTCAGGCTGCAGGAACCTTTTGACTGTTCCAATCCTATTCAGTCTAAAATCTCTCAATCTATCACGGTGCTCAACTATATTCAAATTGCCACCGAGCCTGGCAAACATGGAACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACAGGATTACCCCAGTCCATTTTACTCTTGAAGAAACTTAAAGTGTTATCTTTCTGTGGATGCAAAGGTTTGCAATTGCCTAAATGGTTCTCGGATTTAAGCTCTTTGACATCATTAAATCTACGGAGGTGTAGTCTAGCAGAAGAAGTCCTTGACGGCCTCTACAGCTTATCCGCTCTGCAGATTTTGGATTTGAGTGAAAACAATCTTTCGATCATACCTAGTGGAATTGGTCGCTTATCCTCTTTGAAGCTATTGAATTTGAGTGAGAACAATTTTGACAATATACCAAATGAAATCGGTCACTTATCCTCTTTGCAGGACCGAGTTTATGGCAAAAAACCATTTGAAATTCGTTTTCCACATTCGTGGAGTACAGGACTGAGTGCCGGAATTCCAAATTCGTGGAGTCGTTGGACAAATGGCCCTTCCGTGACAATCCCACTCTCTGATGGTAACAGTACGTGGATGGGACTTGCTCTTTTTGTTGTTTTTGATATCCTTGAGCAAGACATTTTCAACAAGAGCTGGGAATCAAAGGAGACAGTCTGTGATTTTCACACCGATGACGGGTCATATGGTCTTTGTTGCTATGAACCACGTGGTGGGCAATTTAGTGAGTTGTTCGATAAACCAAGTTCACGACTTCGAGCTTCATTTTCAACAAAGAGACCAAATTTAAAAGTTAGAGGTTGTGGGATACATCTAATATCAGAGGAATATGCTGCAGAGTTTGTTAAAAGTATAGCAACTCACACTACTAATTCAACTCAGAGTCATCTAGATTCCAATTTTGATCGACATTGCGAGGACATATTAGATGAGGAAACAACTCGTGTCCTGATAGAACAGGGATCTACTAATTCAACTTTCAATGAAGATAGCTGCAGCAAAGTTAACTCTAAGATCAAACTCAGAGGAGAGCTGTCGATACTCTATGAGGGAAGCAATGGACGTGAGAAGAGTTTCCACTTTTGCTTTCCTGCTTCAGTAATTTCAGCCCTGCCTTGGTTCTTCCATCACCATGCAGGGGATGTAACATTGTGTTACATCACTAAAAACTTCCTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCGACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTATGCTCTCATGATCATGGAAGTATGGTAATGCACGGATCCCTCAAGATAAAGAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAAGGTATCAGCGCATTGTTCAGAACCATGAATGCAGAAATGGAGGTCCAAGTGTGTGGAAGCCGTTTAGCATTTGAGCATGATTTGGAAGACTTGACCCATTCATTAACTGCTGCTGCCGGCACGTTGGGGCAACATGTCCTCACTCAACTATGTTCCCAGGCCCAACCTGTTGATCGGCGCAATGCAGAGGAAGCAGAAATGCCGATCAATTGCTGTTCCTGGTTTAGAAGAAGTACTGCACTTGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGAGGCTGCGAAATCATAGATGCTATCTCCAGCAACAGCAAGGTTCTGGGGAGTCTGACCTTACCCAATTGGTCCACTCCAGATCAGTAACATTACTCCACTCGAAAAGCCTTTTGGAAAACAGGGATAGACATGAAAACGTTGCCGAAGAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGATATAGTTCACCACGGTGGAAGAGATGCCTGAAGTTGTTGCTTCGACAGTCCAAGGTGGCTACTCTCAGTCTTTGTGGACACGCAATTTCAGCTTTCAAGAATTTTGATCCTTTCTCCCCATACAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAACTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCGTCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTTGCATCAATGAAGAGAAACTCAAGTCGCTGCATCTTGGTAGATTCATTTGGCTAACCTACATTCCCCGTATTTTGCTAACCGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAGAGGCCTGACAGTAGAGAAGAGTGGTATACGTCTCTTGTACAGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAGTGCTGGACATCTTTCTTTGATAATCTGTCTTTCATCCGTCAACTTGTAGAAGCAGATGATCAAAATATTCAACCATGGCTAAGACATGAACTTCCGATGCTTGAAGGTCATATCAAGGTCTTTGAACCGGATTTAATATATAATGCAATCCCCCTGTCTAATGAAATTCCGGAGTGGTTCGGGCACCGCATTGAGGACCCCTGGGATGCCAGCTGGGGCTGGCAATTCCAGTTACCACCACCTTTGAGTGACACAAATTGGATAGGATTAGCTCTCTTTGTATCATATCGTATTAGCAGAAATTATTTGCAGGAAAGTGTTTTGTACCCCTTTATTATAACATTGAAAACTGCGGAGAATGGTTTGTCATCTCTCCATCGGTATCAAATGAGCAATGAAGAATTTGAGTTCCTAAAGCGCTGTTGTACTGTGCATCGTGAATTCTTCTGGCTCCCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGTCCTCATTGTTTTATATGGCCTGCACCGGTGGACGCCGGACATGGTCTATCCCCGTTTTGTGTATGCGGATGAGGTGGAAGAGTTTAAGCAGCTCTGCTTCAATCTTCATCGACCCCCTGCCCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

1466

Amino Acids

166.49

Weight (kDa)

6.14

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 108 - 166 4.3e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 152 - 269 4.1e-11 Leucine-rich repeat region
LRR_4 PF12799 290 - 327 2.3e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 302 - 394 4.9e-06 Leucine-rich repeat region
LRR_14 PF23598 412 - 528 9.9e-12 Leucine-rich repeat region
LRR_14 PF23598 506 - 568 3.3e-06 Leucine-rich repeat region
C-JID PF20160 1108 - 1245 3.3e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4103
Acc36I ACCTGC 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 3948
AccB7I CCANNNNNTGG 5 cut(s) 283, 436, 995, 1012, 1991
AccBSI CCGCTC 1 cut(s) 1562
AccI GTMKAC 1 cut(s) 3712
AccIII TCCGGA 1 cut(s) 3934
AciI CCGC 4 cut(s) 1560, 3952, 4118, 4334
AclI AACGTT 1 cut(s) 3105
AcuI CTGAAG 4 cut(s) 178, 2358, 3158, 3233
AcvI CACGTG 2 cut(s) 1988, 2654
AcyI GRCGYC 1 cut(s) 4301
AdeI CACNNNGTG 1 cut(s) 1991
AfeI AGCGCT 1 cut(s) 4184
AflII CTTAAG 1 cut(s) 941
AflIII ACRYGT 3 cut(s) 2644, 2653, 2821
AgeI ACCGGT 1 cut(s) 4293
AhdI GACNNNNNGTC 1 cut(s) 24
AjiI CACGTC 1 cut(s) 2342
AjnI CCWGG 7 cut(s) 186, 225, 490, 1335, 2844, 2906, 3965
AjuI GAANNNNNNNTTGG 2 cut(s) 3062, 3094
AleI CACNNNNGTG 2 cut(s) 1753, 3196
Alw21I GWGCWC 2 cut(s) 114, 1310
Alw26I GTCTC 6 cut(s) 1007, 1083, 1922, 2050, 3158, 3722
Aor13HI TCCGGA 1 cut(s) 3934
Aor51HI AGCGCT 1 cut(s) 4184
AoxI GGCC 8 cut(s) 442, 1219, 1543, 1810, 2847, 3663, 3688, 4285
AsiGI ACCGGT 1 cut(s) 4293
Asp700I GAANNNNTTC 1 cut(s) 2351
AspA2I CCTAGG 1 cut(s) 754
AspLEI GCGC 3 cut(s) 2699, 2868, 4185
AspS9I GGNCC 9 cut(s) 202, 1220, 1708, 1811, 2619, 2731, 2848, 3037, 3961
AsuC2I CCSGG 1 cut(s) 3491
AsuHPI GGTGA 5 cut(s) 248, 812, 977, 2399, 3183
AvaII GGWCC 6 cut(s) 202, 1708, 2619, 2731, 3037, 3961
AvrII CCTAGG 1 cut(s) 754
BaeGI GKGCMC 2 cut(s) 636, 3951
BamHI GGATCC 2 cut(s) 692, 2591
BanI GGYRCC 1 cut(s) 3948
BarI GAAGNNNNNNTAC 2 cut(s) 2497, 2529
BauI CACGAG 2 cut(s) 2220, 3416
BbrPI CACGTG 2 cut(s) 1988, 2654
BbsI GAAGAC 3 cut(s) 479, 2781, 3366
Bbv12I GWGCWC 2 cut(s) 114, 1310
BccI CCATC 8 cut(s) 344, 1073, 1166, 1832, 1849, 2412, 2460, 4149
BceAI ACGGC 3 cut(s) 417, 1558, 2733
BciT130I CCWGG 7 cut(s) 188, 227, 492, 1337, 2846, 2908, 3967
BciVI GTATCC 2 cut(s) 364, 2079
BclI TGATCA 3 cut(s) 2458, 2566, 3823
BcnI CCSGG 1 cut(s) 3491
BcoDI GTCTC 6 cut(s) 1007, 1083, 1922, 2050, 3158, 3722
BfaI CTAG 9 cut(s) 317, 504, 755, 1046, 1523, 1608, 2168, 3378, 3384
BfmI CTRYAG 6 cut(s) 815, 1246, 1549, 1565, 2112, 2272
BfoI RGCGCY 1 cut(s) 4186
BfrI CTTAAG 1 cut(s) 941
BfuAI ACCTGC 1 cut(s) 27
BfuI GTATCC 2 cut(s) 364, 2079
BglII AGATCT 1 cut(s) 734
BlnI CCTAGG 1 cut(s) 754
BmcAI AGTACT 1 cut(s) 2923
Bme1390I CCNGG 8 cut(s) 188, 227, 492, 1337, 2846, 2908, 3491, 3967
Bme18I GGWCC 6 cut(s) 202, 1708, 2619, 2731, 3037, 3961
BmeRI GACNNNNNGTC 1 cut(s) 24
BmgBI CACGTC 1 cut(s) 2342
BmgT120I GGNCC 9 cut(s) 202, 1220, 1708, 1811, 2619, 2731, 2848, 3037, 3961
BmrFI CCNGG 8 cut(s) 188, 227, 492, 1337, 2846, 2908, 3491, 3967
BmrI ACTGGG 2 cut(s) 1053, 1393
BmuI ACTGGG 2 cut(s) 1053, 1393
BoxI GACNNNNGTC 1 cut(s) 3474
BpiI GAAGAC 3 cut(s) 479, 2781, 3366
BpmI CTGGAG 3 cut(s) 2978, 3028, 3451
BpuEI CTTGAG 6 cut(s) 233, 638, 1910, 2582, 3532, 3560
BpuMI CCSGG 1 cut(s) 3491
BsaAI YACGTR 4 cut(s) 551, 1851, 1988, 2654
BsaHI GRCGYC 1 cut(s) 4301
BsaI GGTCTC 1 cut(s) 2050
BsaWI WCCGGW 3 cut(s) 3892, 3934, 4293
BsaXI ACNNNNNCTCC 2 cut(s) 1504, 1534
Bse118I RCCGGY 2 cut(s) 2804, 4293
Bse1I ACTGG 6 cut(s) 25, 1059, 1399, 3338, 3434, 3994
Bse3DI GCAATG 4 cut(s) 2188, 2341, 2875, 4165
BseAI TCCGGA 1 cut(s) 3934
BseBI CCWGG 7 cut(s) 188, 227, 492, 1337, 2846, 2908, 3967
BseMI GCAATG 4 cut(s) 2188, 2341, 2875, 4165
BseMII CTCAG 9 cut(s) 125, 496, 738, 1760, 2171, 2314, 2637, 3153, 3262
BseNI ACTGG 6 cut(s) 25, 1059, 1399, 3338, 3434, 3994
BseRI GAGGAG 4 cut(s) 611, 641, 1373, 2319
BseSI GKGCMC 2 cut(s) 636, 3951
BseYI CCCAGC 2 cut(s) 1914, 3978
BsgI GTGCAG 3 cut(s) 490, 2910, 4274
BshFI GGCC 8 cut(s) 444, 1221, 1545, 1812, 2849, 3665, 3690, 4287
BshNI GGYRCC 1 cut(s) 3948
BshTI ACCGGT 1 cut(s) 4293
BsiHKAI GWGCWC 2 cut(s) 114, 1310
BsiSI CCGG 8 cut(s) 566, 1776, 2805, 3491, 3893, 3935, 4294, 4304
BslFI GGGAC 5 cut(s) 31, 188, 1189, 1872, 2632
BsmAI GTCTC 6 cut(s) 1007, 1083, 1922, 2050, 3158, 3722
BsmBI CGTCTC 1 cut(s) 3722
BsmFI GGGAC 5 cut(s) 31, 188, 1189, 1872, 2632
BsmI GAATGC 2 cut(s) 144, 2722
BsnI GGCC 8 cut(s) 444, 1221, 1545, 1812, 2849, 3665, 3690, 4287
Bso31I GGTCTC 1 cut(s) 2050
Bsp1286I GDGCHC 4 cut(s) 114, 636, 1310, 3951
Bsp13I TCCGGA 1 cut(s) 3934
Bsp1407I TGTACA 4 cut(s) 58, 2504, 3148, 3723
Bsp19I CCATGG 2 cut(s) 679, 3839
BspACI CCGC 4 cut(s) 1560, 3952, 4118, 4334
BspANI GGCC 8 cut(s) 444, 1221, 1545, 1812, 2849, 3665, 3690, 4287
BspEI TCCGGA 1 cut(s) 3934
BspHI TCATGA 2 cut(s) 2563, 3466
BspMAI CTGCAG 5 cut(s) 819, 1250, 1569, 2116, 2276
BspMI ACCTGC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 3948
BspTI CTTAAG 1 cut(s) 941
BspTNI GGTCTC 1 cut(s) 2050
BsrBI CCGCTC 1 cut(s) 1562
BsrDI GCAATG 4 cut(s) 2188, 2341, 2875, 4165
BsrFI RCCGGY 2 cut(s) 2804, 4293
BsrGI TGTACA 4 cut(s) 58, 2504, 3148, 3723
BsrI ACTGG 6 cut(s) 25, 1059, 1399, 3338, 3434, 3994
BssAI RCCGGY 2 cut(s) 2804, 4293
BssNAI GTATAC 1 cut(s) 3713
BssNI GRCGYC 1 cut(s) 4301
BssSI CACGAG 2 cut(s) 2220, 3416
BssT1I CCWWGG 6 cut(s) 679, 754, 2393, 3234, 3519, 3839
Bst1107I GTATAC 1 cut(s) 3713
Bst2BI CACGAG 2 cut(s) 2220, 3416
Bst2UI CCWGG 7 cut(s) 188, 227, 492, 1337, 2846, 2908, 3967
Bst6I CTCTTC 6 cut(s) 2342, 3197, 3561, 3698, 3729, 4341
BstACI GRCGYC 1 cut(s) 4301
BstAFI CTTAAG 1 cut(s) 941
BstAUI TGTACA 4 cut(s) 58, 2504, 3148, 3723
BstBAI YACGTR 4 cut(s) 551, 1851, 1988, 2654
BstC8I GCNNGC 5 cut(s) 110, 2806, 3976, 3986, 4289
BstDSI CCRYGG 3 cut(s) 679, 3194, 3839
BstH2I RGCGCY 1 cut(s) 4186
BstHHI GCGC 3 cut(s) 2699, 2868, 4185
BstMAI GTCTC 6 cut(s) 1007, 1083, 1922, 2050, 3158, 3722
BstMWI GCNNNNNNNGC 4 cut(s) 779, 1551, 2879, 3272
BstNI CCWGG 7 cut(s) 188, 227, 492, 1337, 2846, 2908, 3967
BstNSI RCATGY 4 cut(s) 853, 1090, 2648, 2825
BstPAI GACNNNNGTC 1 cut(s) 3474
BstSCI CCNGG 8 cut(s) 186, 225, 490, 1335, 2844, 2906, 3489, 3965
BstSFI CTRYAG 6 cut(s) 815, 1246, 1549, 1565, 2112, 2272
BstSLI GKGCMC 2 cut(s) 636, 3951
BstV2I GAAGAC 3 cut(s) 479, 2781, 3366
BstX2I RGATCY 7 cut(s) 154, 271, 692, 734, 912, 2240, 2591
BstXI CCANNNNNNTGG 5 cut(s) 187, 1755, 1791, 2741, 4236
BstYI RGATCY 7 cut(s) 154, 271, 692, 734, 912, 2240, 2591
BstZ17I GTATAC 1 cut(s) 3713
BsuI GTATCC 2 cut(s) 364, 2079
BsuRI GGCC 8 cut(s) 444, 1221, 1545, 1812, 2849, 3665, 3690, 4287
BtgI CCRYGG 3 cut(s) 679, 3194, 3839
BtrI CACGTC 1 cut(s) 2342
BtsI GCAGTG 1 cut(s) 812
BtsIMutI CAGTG 4 cut(s) 518, 812, 3345, 3649
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 5 cut(s) 110, 2806, 3976, 3986, 4289
CciI TCATGA 2 cut(s) 2563, 3466
CfoI GCGC 3 cut(s) 2699, 2868, 4185
Cfr10I RCCGGY 2 cut(s) 2804, 4293
Cfr13I GGNCC 9 cut(s) 202, 1220, 1708, 1811, 2619, 2731, 2848, 3037, 3961
CseI GACGC 3 cut(s) 3464, 4242, 4309
CspAI ACCGGT 1 cut(s) 4293
CspCI CAANNNNNGTGG 2 cut(s) 3990, 4025
DraI TTTAAA 1 cut(s) 2067
DraIII CACNNNGTG 1 cut(s) 1991
DriI GACNNNNNGTC 1 cut(s) 24
Eam1104I CTCTTC 6 cut(s) 2342, 3197, 3561, 3698, 3729, 4341
Eam1105I GACNNNNNGTC 1 cut(s) 24
EarI CTCTTC 6 cut(s) 2342, 3197, 3561, 3698, 3729, 4341
Eco130I CCWWGG 6 cut(s) 679, 754, 2393, 3234, 3519, 3839
Eco147I AGGCCT 2 cut(s) 444, 3690
Eco31I GGTCTC 1 cut(s) 2050
Eco32I GATATC 1 cut(s) 1885
Eco47I GGWCC 6 cut(s) 202, 1708, 2619, 2731, 3037, 3961
Eco47III AGCGCT 1 cut(s) 4184
Eco57I CTGAAG 4 cut(s) 178, 2358, 3158, 3233
Eco72I CACGTG 2 cut(s) 1988, 2654
EcoO109I RGGNCCY 3 cut(s) 202, 2619, 3961
EcoRI GAATTC 3 cut(s) 1197, 1779, 4205
EcoRII CCWGG 7 cut(s) 186, 225, 490, 1335, 2844, 2906, 3965
EcoRV GATATC 1 cut(s) 1885
EcoT14I CCWWGG 6 cut(s) 679, 754, 2393, 3234, 3519, 3839
EcoT22I ATGCAT 1 cut(s) 1212
ErhI CCWWGG 6 cut(s) 679, 754, 2393, 3234, 3519, 3839
Esp3I CGTCTC 1 cut(s) 3722
FalI AAGNNNNNCTT 2 cut(s) 1521, 1553
FaqI GGGAC 5 cut(s) 31, 188, 1189, 1872, 2632
FauNDI CATATG 1 cut(s) 1963
FbaI TGATCA 3 cut(s) 2458, 2566, 3823
FblI GTMKAC 1 cut(s) 3712
FspBI CTAG 9 cut(s) 317, 504, 755, 1046, 1523, 1608, 2168, 3378, 3384
GlaI GCGC 3 cut(s) 2698, 2867, 4184
GsaI CCCAGC 2 cut(s) 1918, 3982
GsuI CTGGAG 3 cut(s) 2978, 3028, 3451
HaeII RGCGCY 1 cut(s) 4186
HaeIII GGCC 8 cut(s) 444, 1221, 1545, 1812, 2849, 3665, 3690, 4287
HapII CCGG 8 cut(s) 566, 1776, 2805, 3491, 3893, 3935, 4294, 4304
HgaI GACGC 3 cut(s) 3464, 4242, 4309
HhaI GCGC 3 cut(s) 2699, 2868, 4185
Hin1I GRCGYC 1 cut(s) 4301
Hin6I GCGC 3 cut(s) 2697, 2866, 4183
HinP1I GCGC 3 cut(s) 2697, 2866, 4183
HincII GTYRAC 3 cut(s) 29, 2284, 3806
HindII GTYRAC 3 cut(s) 29, 2284, 3806
HindIII AAGCTT 1 cut(s) 209
HpaI GTTAAC 1 cut(s) 2284
HpaII CCGG 8 cut(s) 566, 1776, 2805, 3491, 3893, 3935, 4294, 4304
HphI GGTGA 5 cut(s) 248, 812, 977, 2399, 3183
Hpy99I CGWCG 1 cut(s) 4236
HpyF10VI GCNNNNNNNGC 4 cut(s) 779, 1551, 2879, 3272
Hsp92I GRCGYC 1 cut(s) 4301
HspAI GCGC 3 cut(s) 2697, 2866, 4183
Kpn2I TCCGGA 1 cut(s) 3934
KroI GCCGGC 1 cut(s) 2804
KroNI GCCGGC 1 cut(s) 2806
Ksp22I TGATCA 3 cut(s) 2458, 2566, 3823
KspAI GTTAAC 1 cut(s) 2284
LmnI GCTCC 6 cut(s) 412, 598, 779, 1360, 2953, 4221
MaeI CTAG 9 cut(s) 317, 504, 755, 1046, 1523, 1608, 2168, 3378, 3384
MbiI CCGCTC 1 cut(s) 1562
MfeI CAATTG 5 cut(s) 264, 1460, 2896, 2961, 3032
MflI RGATCY 7 cut(s) 154, 271, 692, 734, 912, 2240, 2591
MhlI GDGCHC 4 cut(s) 114, 636, 1310, 3951
MlyI GAGTC 5 cut(s) 1804, 2170, 3026, 3160, 4271
MmeI TCCRAC 5 cut(s) 301, 915, 1030, 1781, 3503
Mph1103I ATGCAT 1 cut(s) 1212
MroI TCCGGA 1 cut(s) 3934
MroNI GCCGGC 1 cut(s) 2804
MroXI GAANNNNTTC 1 cut(s) 2351
MslI CAYNNNNRTG 8 cut(s) 684, 1170, 1753, 1950, 2409, 2568, 3196, 3420
MspA1I CMGCKG 1 cut(s) 3978
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 8 cut(s) 566, 1776, 2805, 3491, 3893, 3935, 4294, 4304
MspR9I CCNGG 8 cut(s) 188, 227, 492, 1337, 2846, 2908, 3491, 3967
MunI CAATTG 5 cut(s) 264, 1460, 2896, 2961, 3032
Mva1269I GAATGC 2 cut(s) 144, 2722
MvaI CCWGG 7 cut(s) 188, 227, 492, 1337, 2846, 2908, 3967
MwoI GCNNNNNNNGC 4 cut(s) 779, 1551, 2879, 3272
NaeI GCCGGC 1 cut(s) 2806
NciI CCSGG 1 cut(s) 3491
NcoI CCATGG 2 cut(s) 679, 3839
NdeI CATATG 1 cut(s) 1963
NgoMIV GCCGGC 1 cut(s) 2804
NmuCI GTSAC 6 cut(s) 537, 1155, 1688, 1819, 3506, 4013
NsiI ATGCAT 1 cut(s) 1212
NspI RCATGY 4 cut(s) 853, 1090, 2648, 2825
OliI CACNNNNGTG 2 cut(s) 1753, 3196
PagI TCATGA 2 cut(s) 2563, 3466
PasI CCCWGGG 1 cut(s) 3966
PceI AGGCCT 2 cut(s) 444, 3690
PciI ACATGT 2 cut(s) 2644, 2821
PctI GAATGC 2 cut(s) 144, 2722
PdiI GCCGGC 1 cut(s) 2806
PdmI GAANNNNTTC 1 cut(s) 2351
PfeI GAWTC 8 cut(s) 638, 658, 1919, 2171, 2677, 3393, 3538, 3597
PflFI GACNNNGTC 1 cut(s) 4310
PflMI CCANNNNNTGG 5 cut(s) 283, 436, 995, 1012, 1991
PfoI TCCNGGA 1 cut(s) 225
PinAI ACCGGT 1 cut(s) 4293
PleI GAGTC 5 cut(s) 1803, 2169, 3025, 3160, 4270
PmaCI CACGTG 2 cut(s) 1988, 2654
PmlI CACGTG 2 cut(s) 1988, 2654
PpsI GAGTC 5 cut(s) 1803, 2169, 3025, 3160, 4270
Ppu21I YACGTR 4 cut(s) 551, 1851, 1988, 2654
PpuMI RGGWCCY 3 cut(s) 202, 2619, 3961
PscI ACATGT 2 cut(s) 2644, 2821
PshAI GACNNNNGTC 1 cut(s) 3474
PsiI TTATAA 1 cut(s) 4103
Psp1406I AACGTT 1 cut(s) 3105
Psp5II RGGWCCY 3 cut(s) 202, 2619, 3961
Psp6I CCWGG 7 cut(s) 186, 225, 490, 1335, 2844, 2906, 3965
PspCI CACGTG 2 cut(s) 1988, 2654
PspFI CCCAGC 2 cut(s) 1914, 3978
PspGI CCWGG 7 cut(s) 186, 225, 490, 1335, 2844, 2906, 3965
PspPI GGNCC 9 cut(s) 202, 1220, 1708, 1811, 2619, 2731, 2848, 3037, 3961
PspPPI RGGWCCY 3 cut(s) 202, 2619, 3961
PsrI GAACNNNNNNTAC 2 cut(s) 2640, 2672
PstI CTGCAG 5 cut(s) 819, 1250, 1569, 2116, 2276
PsuI RGATCY 7 cut(s) 154, 271, 692, 734, 912, 2240, 2591
PsyI GACNNNGTC 1 cut(s) 4310
PvuII CAGCTG 1 cut(s) 3978
RseI CAYNNNNRTG 8 cut(s) 684, 1170, 1753, 1950, 2409, 2568, 3196, 3420
Sau96I GGNCC 9 cut(s) 202, 1220, 1708, 1811, 2619, 2731, 2848, 3037, 3961
ScaI AGTACT 1 cut(s) 2923
SchI GAGTC 5 cut(s) 1804, 2170, 3026, 3160, 4271
ScrFI CCNGG 8 cut(s) 188, 227, 492, 1337, 2846, 2908, 3491, 3967
SduI GDGCHC 4 cut(s) 114, 636, 1310, 3951
SfcI CTRYAG 6 cut(s) 815, 1246, 1549, 1565, 2112, 2272
SgrAI CRCCGGYG 1 cut(s) 4293
SinI GGWCC 6 cut(s) 202, 1708, 2619, 2731, 3037, 3961
SmiMI CAYNNNNRTG 8 cut(s) 684, 1170, 1753, 1950, 2409, 2568, 3196, 3420
SmlI CTYRAG 7 cut(s) 212, 653, 941, 1889, 2597, 3511, 3575
SmoI CTYRAG 7 cut(s) 212, 653, 941, 1889, 2597, 3511, 3575
SseBI AGGCCT 2 cut(s) 444, 3690
SsiI CCGC 4 cut(s) 1560, 3952, 4118, 4334
SspI AATATT 2 cut(s) 3310, 3832
SspMI CTAG 9 cut(s) 317, 504, 755, 1046, 1523, 1608, 2168, 3378, 3384
StuI AGGCCT 2 cut(s) 444, 3690
StyD4I CCNGG 8 cut(s) 186, 225, 490, 1335, 2844, 2906, 3489, 3965
StyI CCWWGG 6 cut(s) 679, 754, 2393, 3234, 3519, 3839
TaqII GACCGA 2 cut(s) 1676, 1725
TatI WGTACW 8 cut(s) 58, 1760, 2504, 2921, 2932, 3148, 3723, 4190
TfiI GAWTC 8 cut(s) 638, 658, 1919, 2171, 2677, 3393, 3538, 3597
TscAI CASTG 4 cut(s) 525, 819, 3345, 3649
TseFI GTSAC 6 cut(s) 537, 1155, 1688, 1819, 3506, 4013
Tsp45I GTSAC 6 cut(s) 537, 1155, 1688, 1819, 3506, 4013
TspGWI ACGGA 4 cut(s) 1525, 1807, 2604, 3791
TspRI CASTG 4 cut(s) 525, 819, 3345, 3649
Tth111I GACNNNGTC 1 cut(s) 4310
Van91I CCANNNNNTGG 5 cut(s) 283, 436, 995, 1012, 1991
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 6 cut(s) 202, 1708, 2619, 2731, 3037, 3961
XbaI TCTAGA 3 cut(s) 316, 1045, 2167
XceI RCATGY 4 cut(s) 853, 1090, 2648, 2825
XcmI CCANNNNNNNNNTGG 1 cut(s) 686
XmaJI CCTAGG 1 cut(s) 754
XmiI GTMKAC 1 cut(s) 3712
XmnI GAANNNNTTC 1 cut(s) 2351
XspI CTAG 9 cut(s) 317, 504, 755, 1046, 1523, 1608, 2168, 3378, 3384
ZrmI AGTACT 1 cut(s) 2923
Zsp2I ATGCAT 1 cut(s) 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.