Rmu_sc0005255.1_g000019

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005255.1
OG0000307
Physical Location & Seq
Forward (+)
84914 .. 93304
8391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005255.1_g000019.1.cds

Sequence Viewer

Length: 5130 bp
atgcttcggtggcagctggctggaacgcagggctgcaggaagggggcagcaggggctgctgtgcaaggttgcaagcacacgggcgctcgggctgcagcgaaggctcggctagctcgggctaggggctgctttgtgaatgagttttggttttctgttttgtggtcagaggcagaagttattcaagaaatagctagaaagatttcaaaaatgttgccctttatggtctctagtgctagcaaaaatattgttggaatcgatgttcgtatagagacattgaagaattacttggctttgtggtccatatatgacattcgcaccatagggatttggggaatggggggcataggtaaaacaactcttgcagcagaatttttcaaaagatttcgtaaccaatttgatgatagcgcgtttgttgccaatgttagattggaagcgcggaaaggtcaattttacttgcaaaaacttctttatgcatccttcttggagagtgatgataatacatcgtccgttcagaaagggaatgaattcttatggagaatgctaaatcaaaaggttcttatcattttggacgatgttgatgaattaaaacaaatagaagatttagcaggcggaaggccaggtgcatttaattggttgggtcgaggaagtcgagtgattataacaactagagataagcagccgttgttgacatggggagtccaagaagagaacatatacgaggttgagaaactgactgatgatgaagctcttcagcttttatgtcaaaaagcattcaacagtgaacatgctccagatggttataaagagctgtccaacaagttgttaaaatatgttagtggccttcctctagctattgaagttcttggttcatacttgtctgggagacaggtaagtgaatggtcggaggcattggctagacttcatgaagatccagaaaaaggcattttcagtgttcttcaaataagttttgatggattaagggaaactgagaagaatatctttttggatattgcatgtttcttcagaggcgaggatcaagcacctgtaaggaagatattaaaaagctgtggcttttttcctgaaaacgaatctcttcaagatcctggcaaacgtagtaggttgtggcttaatgataatacctacttgtacgaacgcaatgaatcgtggcttgacaaggatgcctgtgatgtactcacagaaaatacgggaacgaaagctgttgaaggcatattcctacgcttccctgaaaaattggaaatctgcttaaatgctgacccgttctctaatatgcgctacctcagattgttgaagatttgtaatgtcaaatttctgggtgctccttttatacatctctctaaacacttaagagttttggagtggcatgaatgtcctttagaatctctgccatcaagttgtcaaccggatcggcttgttgaacttaagatgcctaatagccgcattgaacaactatggaatgaaagggagcttgatacctggagcactctgaattggagttgcagtttccgtgaaacagatttaagaaataattatcatttgatgctttgtgacaattctcctcagatgttctttgattcctgtcaatattcatctttgacatctttacttctgagtggatgcaagctaggcgaaggagcaatccctgatgatattggctgcttatactcactccatttgttagacttgagtgaaaacaatttcgtgagcatacctgaaaccatttctcaactttccaagctcagaaagcttctgttgtttaggtgcagcaaacttcagtatttgccaaaacaacttccactgagtttaaaacgtgtaattgcacgcaattgtccgatgctgagaactacttatccaaatactatcgtaaaatggacttcagatgaaggtttcagtttcatagattgcagatattcagacgaaaatgctgacgatgatgaaggatatatgaatttccataagaacattgaggatcgaattcatcatgacaagccatttgaaattcgttttggtgatagtcgtgctgacttttctaatgtctggtgcactcagtggaacactggttcttctgttacactgcaactaactggtccagaagacaagactaatagtacgtggatgggattagccttatttgttgcttttgaactcattccgaatggtcaagtgaaagaaactgtctgtcatctgagcatcgacgaaggtggtccccaaattcgtttagtttttgaagacttggattatgagtcacttggactctgttgctacttgccacgaggctgcggggggtttgcaggacagctggataaagccagtcgaattaatgctacatttttgccaactacttcactggtgaatgcgtgtgggatacatccaatatccgagcatgacgctccaatgtttgctcaggagttagccgggactgcttttgctgacgcacgtgaagtttgtcgacatggtaagagaatgttagataaggcaacgaaattggaaaggtcgggagacgtgctggtacctgcgtacaattctttcaatgtgaatcgacacaagtggaaaattgatcctgatatccatcccagattagaggaagagctaaagctgttcagattgctttcagggctctacgagggaagcaatggacgtcagaagaaatttcatttttgttttccagatcaagtaatttcgccatggttcatcaatcactacgcagggaatgtagcactttgtgaccttcctcagaacttaatcgatgataagagatggattggattagaagtgtatgttgtgtttacacctggttacagtgaccacaatctttctgattccttccttcatgttgatttaatggcacatgaaagtagtgtaatgcattcgtccatcaaaattgagagttgttctgggtcatgtcaacttattgtactacatgtaccacggatgcattttccagaacagttgactcattatcaggttattagcacgttgtttacaaccagtaccccaattgttgagattatagtgtgcgggagccgattggtatatgagcatgatctggaagatttaatcctttcattaactgcgtgtacattgaggaagccatattcaattggccaagtttattcccaggcccaaacatatgttgaaactatggaaaagaaagcagatgaaaatatccagtgcaatgcaaagcgaggagaaatgtcatccactgatggccattcatcgtttcaaagaagtgtcaaacaaattgaagtaccagataaagttcgcagttcaatattgcagaaatatagatgccatatccaaggaaagcaaggtatactagagtctgacaaaataattgtccactctaaatcccttcttctggacaataccatcaaagaaaattttactggaaaagaatcaataatgtcgtcgttggcatgtcatcatttgcatatagtggctgagactcacttctggggaagctatagtgcccgacagtggaagagacgtttagagttgttgctgaaatattctgaggtggcaactctcagtcttaatggacatgtaatctctgtaatgaagcattttagtccctcctcgccattcaatttttgtttccctaaagatgaagtgctgctagactggatgagaggtcgtcaattccgattgcgtcaacaactctacgatgataaaagtttcaggggacttgttatatttgcaactttttcatttgacgattatcctgccatgtacaatctgccttcagaaattactgttgaactcttgtgtcacttgagttacaatggctccaggttttgcgtgaacgctgcccccatgcttacgatcactaaggagaaattccagtggctttttttccgtgatttcatttgggttacgtatgtaccccttgttatgctagcggaggtcaaagtaaccagtgatataggggctgatatatgtagtaattgcccaggcttgagggtggaccgcgcttatacatgtgtcttgcgccggcaagaggaggaggaatttaagcaaaaaataactcagtgctggacctccttctttgataatatcgatctcctccgtcggtttgtacaagcagatgctgaaaaaggtgtagagacgaagcacgaacttccaatgctcggcggaagtatgaaggaccttgatagcaacttgatgtatgacgcaacacacccatttactgaaattccggattggtttgaacatccaaatgaaaggtttggagagatccagttaccaccatctcttggcagggacgaaaattggataggagtggctatatgtgcatcatattttcccttacaattgaatcctcaagtttctccgtacttagaatgtgttttgcgaactaagatgagtgacttggaatctgtccaccgctatcagatgaccaatgaagaagtcaagtgcgtatcttccaagagaaaattcatttggctatcctatcttccacgacactggtttttccaccagttaaatgatgagtctgctctcagggcttcgttttcaagttcacctggtttgtggtttgaggagcgcggagagcgcgacgttgaaatcgaacgccgctttgcgctcggcgaaccagcgtggggcttcgcaaatggtgatacttcaaagacaactggccaagaatttctggtccagcccattcatcagagtctaccagttcatcaggctcagaacccttaccctcagaccctgcagaaacatggcattcttccactagttgtagagtcagcattggctttgcaagaagctgggtgcttttctgttgttttggaatgtgcaccgccactagtggcactgcagcaacgtctgctcttcagattcctacatttggcattggagcagtgcctttttgcagtggacaggtcagttcattatgtagccatgttgatcatggttggagatgtcatcaacaaagctcttgtagactacaaggagactagtggaacattcccaggtgctgcccgcagcccatacaagattaaccctgctgatgttgatggctttttaaacgagttacgaaaaatgggtttggacaaggctgcatctgcggtggctgaagcagctgagaagataaatacaaacaaatcaaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1709

Amino Acids

194.9

Weight (kDa)

6.04

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 659, 801
AatII GACGTC 1 cut(s) 2659
Acc36I ACCTGC 1 cut(s) 2539
Acc65I GGTACC 1 cut(s) 2527
AccB1I GGYRCC 1 cut(s) 2527
AccB7I CCANNNNNTGG 1 cut(s) 4271
AccI GTMKAC 4 cut(s) 2467, 3331, 4687, 4959
AccII CGCG 5 cut(s) 407, 436, 3987, 4563, 4572
AccIII TCCGGA 1 cut(s) 4213
AclWI GGATC 7 cut(s) 923, 1041, 1094, 1431, 1995, 2570, 4246
AcoI YGGCCR 3 cut(s) 3121, 3226, 4651
AcuI CTGAAG 7 cut(s) 734, 1006, 1775, 1878, 3744, 4834, 5112
AcvI CACGTG 1 cut(s) 2456
AcyI GRCGYC 1 cut(s) 2656
AdeI CACNNNGTG 2 cut(s) 2067, 2741
AfiI CCNNNNNNNGG 8 cut(s) 938, 3376, 3495, 4015, 4145, 4271, 4616, 4864
AflII CTTAAG 2 cut(s) 1363, 1439
AflIII ACRYGT 4 cut(s) 1828, 2938, 3559, 3995
AhlI ACTAGT 3 cut(s) 4750, 4822, 4973
AjiI CACGTC 1 cut(s) 2521
AjnI CCWGG 9 cut(s) 616, 1102, 1493, 2809, 3135, 3806, 3967, 4540, 4987
AloI GAACNNNNNNTCC 6 cut(s) 243, 275, 1852, 1884, 2062, 2094
Alw21I GWGCWC 4 cut(s) 1339, 1502, 2063, 4816
Alw26I GTCTC 8 cut(s) 229, 263, 877, 2511, 3455, 3496, 4115, 4964
Alw44I GTGCAC 2 cut(s) 2059, 4812
AlwI GGATC 7 cut(s) 923, 1041, 1094, 1431, 1995, 2570, 4246
AlwNI CAGNNNCTG 4 cut(s) 56, 4106, 4726, 4994
Ama87I CYCGRG 2 cut(s) 87, 114
Aor13HI TCCGGA 1 cut(s) 4213
AoxI GGCC 6 cut(s) 614, 838, 3121, 3138, 3226, 4651
ApaLI GTGCAC 2 cut(s) 2059, 4812
ArsI GACNNNNNNTTYG 4 cut(s) 1994, 2026, 3339, 3371
AseI ATTAAT 1 cut(s) 2337
Asp700I GAANNNNTTC 6 cut(s) 177, 199, 524, 747, 1092, 2166
Asp718I GGTACC 1 cut(s) 2527
AspLEI GCGC 9 cut(s) 86, 407, 436, 1293, 3989, 4008, 4563, 4572, 4600
AspS9I GGNCC 8 cut(s) 297, 2105, 2222, 3139, 3982, 4053, 4162, 4666
AsuC2I CCSGG 1 cut(s) 2434
AsuHPI GGTGA 4 cut(s) 2039, 2380, 4530, 4643
AsuNHI GCTAGC 3 cut(s) 109, 233, 3913
AvaI CYCGRG 2 cut(s) 87, 114
AvaII GGWCC 7 cut(s) 297, 2105, 2222, 3982, 4053, 4162, 4666
BaeGI GKGCMC 3 cut(s) 2063, 3490, 4816
BaeI ACNNNNGTAYC 4 cut(s) 2519, 2552, 3882, 3915
BalI TGGCCA 3 cut(s) 3123, 3228, 4653
BanI GGYRCC 1 cut(s) 2527
BanII GRGCYC 1 cut(s) 2637
BauI CACGAG 1 cut(s) 2289
BbrPI CACGTG 1 cut(s) 2456
BbsI GAAGAC 2 cut(s) 2118, 2253
Bbv12I GWGCWC 4 cut(s) 1339, 1502, 2063, 4816
BceAI ACGGC 1 cut(s) 664
BcgI CGANNNNNNTGC 2 cut(s) 3722, 3756
BciT130I CCWGG 9 cut(s) 618, 1104, 1495, 2811, 3137, 3808, 3969, 4542, 4989
BciVI GTATCC 1 cut(s) 2376
BclI TGATCA 1 cut(s) 4923
BcnI CCSGG 1 cut(s) 2434
BcoDI GTCTC 8 cut(s) 229, 263, 877, 2511, 3455, 3496, 4115, 4964
BcuI ACTAGT 3 cut(s) 4750, 4822, 4973
BfmI CTRYAG 5 cut(s) 34, 93, 3481, 4727, 4832
BfoI RGCGCY 1 cut(s) 87
BfrI CTTAAG 2 cut(s) 1363, 1439
BfuAI ACCTGC 1 cut(s) 2539
BfuI GTATCC 1 cut(s) 2376
Bme18I GGWCC 7 cut(s) 297, 2105, 2222, 3982, 4053, 4162, 4666
BmeT110I CYCGRG 2 cut(s) 87, 114
BmgBI CACGTC 1 cut(s) 2521
BmgT120I GGNCC 8 cut(s) 297, 2105, 2222, 3139, 3982, 4053, 4162, 4666
BmiI GGNNCC 4 cut(s) 2224, 2529, 3041, 3805
BmtI GCTAGC 3 cut(s) 113, 237, 3917
BpiI GAAGAC 2 cut(s) 2118, 2253
BpmI CTGGAG 3 cut(s) 774, 1516, 3790
Bpu10I CCTNAGC 1 cut(s) 2421
BpuEI CTTGAG 4 cut(s) 1723, 3811, 3994, 4323
BpuMI CCSGG 1 cut(s) 2434
Bsa29I ATCGAT 3 cut(s) 255, 2763, 4074
BsaAI YACGTR 3 cut(s) 2130, 2456, 3894
BsaBI GATNNNNATC 1 cut(s) 2586
BsaHI GRCGYC 1 cut(s) 2656
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 6 cut(s) 2702, 2945, 3135, 3316, 3967, 4987
BsaWI WCCGGW 2 cut(s) 1420, 4213
BsaXI ACNNNNNCTCC 4 cut(s) 1504, 1534, 3788, 3818
Bsc4I CCNNNNNNNGG 8 cut(s) 938, 3376, 3495, 4015, 4145, 4271, 4616, 4864
Bse118I RCCGGY 1 cut(s) 4008
Bse3DI GCAATG 3 cut(s) 1162, 2656, 3199
Bse8I GATNNNNATC 1 cut(s) 2586
BseAI TCCGGA 1 cut(s) 4213
BseBI CCWGG 9 cut(s) 618, 1104, 1495, 2811, 3137, 3808, 3969, 4542, 4989
BseCI ATCGAT 3 cut(s) 255, 2763, 4074
BseDI CCNNGG 6 cut(s) 2702, 2945, 3135, 3316, 3967, 4987
BseJI GATNNNNATC 1 cut(s) 2586
BseLI CCNNNNNNNGG 8 cut(s) 938, 3376, 3495, 4015, 4145, 4271, 4616, 4864
BseMI GCAATG 3 cut(s) 1162, 2656, 3199
BseRI GAGGAG 7 cut(s) 1566, 3219, 3583, 4031, 4034, 4070, 4571
BseSI GKGCMC 3 cut(s) 2063, 3490, 4816
BseYI CCCAGC 1 cut(s) 4784
BsgI GTGCAG 1 cut(s) 1801
Bsh1236I CGCG 5 cut(s) 407, 436, 3987, 4563, 4572
BshFI GGCC 6 cut(s) 616, 840, 3123, 3140, 3228, 4653
BshNI GGYRCC 1 cut(s) 2527
BshVI ATCGAT 3 cut(s) 255, 2763, 4074
BsiHKAI GWGCWC 4 cut(s) 1339, 1502, 2063, 4816
BsiHKCI CYCGRG 2 cut(s) 87, 114
BsiSI CCGG 4 cut(s) 1421, 2433, 4009, 4214
BslFI GGGAC 5 cut(s) 2208, 2449, 3573, 3714, 4292
BslI CCNNNNNNNGG 8 cut(s) 938, 3376, 3495, 4015, 4145, 4271, 4616, 4864
BsmAI GTCTC 8 cut(s) 229, 263, 877, 2511, 3455, 3496, 4115, 4964
BsmBI CGTCTC 3 cut(s) 2511, 3496, 4115
BsmFI GGGAC 5 cut(s) 2208, 2449, 3573, 3714, 4292
BsmI GAATGC 5 cut(s) 543, 770, 2377, 2884, 4740
BsnI GGCC 6 cut(s) 616, 840, 3123, 3140, 3228, 4653
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 2 cut(s) 87, 114
Bsp1286I GDGCHC 6 cut(s) 1339, 1502, 2063, 2637, 3490, 4816
Bsp13I TCCGGA 1 cut(s) 4213
Bsp1407I TGTACA 3 cut(s) 3095, 3747, 4093
Bsp19I CCATGG 1 cut(s) 2702
BspANI GGCC 6 cut(s) 616, 840, 3123, 3140, 3228, 4653
BspDI ATCGAT 3 cut(s) 255, 2763, 4074
BspEI TCCGGA 1 cut(s) 4213
BspFNI CGCG 5 cut(s) 407, 436, 3987, 4563, 4572
BspHI TCATGA 2 cut(s) 922, 1999
BspLI GGNNCC 4 cut(s) 2224, 2529, 3041, 3805
BspMAI CTGCAG 4 cut(s) 38, 97, 4731, 4836
BspMI ACCTGC 1 cut(s) 2539
BspOI GCTAGC 3 cut(s) 113, 237, 3917
BspPI GGATC 7 cut(s) 923, 1041, 1094, 1431, 1995, 2570, 4246
BspQI GCTCTTC 3 cut(s) 753, 2598, 4853
BspT107I GGYRCC 1 cut(s) 2527
BspTI CTTAAG 2 cut(s) 1363, 1439
BspTNI GGTCTC 1 cut(s) 229
BsrDI GCAATG 3 cut(s) 1162, 2656, 3199
BsrFI RCCGGY 1 cut(s) 4008
BsrGI TGTACA 3 cut(s) 3095, 3747, 4093
BssAI RCCGGY 1 cut(s) 4008
BssECI CCNNGG 6 cut(s) 2702, 2945, 3135, 3316, 3967, 4987
BssNAI GTATAC 1 cut(s) 3332
BssNI GRCGYC 1 cut(s) 2656
BssSI CACGAG 1 cut(s) 2289
BssT1I CCWWGG 2 cut(s) 2702, 3316
Bst1107I GTATAC 1 cut(s) 3332
Bst2BI CACGAG 1 cut(s) 2289
Bst2UI CCWGG 9 cut(s) 618, 1104, 1495, 2811, 3137, 3808, 3969, 4542, 4989
Bst4CI ACNGT 6 cut(s) 779, 2194, 2819, 2967, 3495, 3772
Bst6I CTCTTC 6 cut(s) 699, 753, 1098, 2598, 3494, 4853
BstACI GRCGYC 1 cut(s) 2656
BstAFI CTTAAG 2 cut(s) 1363, 1439
BstAPI GCANNNNNTGC 2 cut(s) 56, 4843
BstAUI TGTACA 3 cut(s) 3095, 3747, 4093
BstBAI YACGTR 3 cut(s) 2130, 2456, 3894
BstDSI CCRYGG 2 cut(s) 2702, 2945
BstFNI CGCG 5 cut(s) 407, 436, 3987, 4563, 4572
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 9 cut(s) 86, 407, 436, 1293, 3989, 4008, 4563, 4572, 4600
BstMAI GTCTC 8 cut(s) 229, 263, 877, 2511, 3455, 3496, 4115, 4964
BstNI CCWGG 9 cut(s) 618, 1104, 1495, 2811, 3137, 3808, 3969, 4542, 4989
BstNSI RCATGY 6 cut(s) 788, 1017, 2942, 3438, 3563, 3999
BstSFI CTRYAG 5 cut(s) 34, 93, 3481, 4727, 4832
BstSLI GKGCMC 3 cut(s) 2063, 3490, 4816
BstSNI TACGTA 1 cut(s) 3894
BstUI CGCG 5 cut(s) 407, 436, 3987, 4563, 4572
BstV2I GAAGAC 2 cut(s) 2118, 2253
BstX2I RGATCY 3 cut(s) 928, 1099, 4251
BstXI CCANNNNNNTGG 1 cut(s) 4482
BstYI RGATCY 3 cut(s) 928, 1099, 4251
BstZ17I GTATAC 1 cut(s) 3332
Bsu15I ATCGAT 3 cut(s) 255, 2763, 4074
BsuI GTATCC 1 cut(s) 2376
BsuRI GGCC 6 cut(s) 616, 840, 3123, 3140, 3228, 4653
BsuTUI ATCGAT 3 cut(s) 255, 2763, 4074
BtgI CCRYGG 2 cut(s) 2702, 2945
BtrI CACGTC 1 cut(s) 2521
BtsI GCAGTG 4 cut(s) 2090, 4829, 4883, 4896
BveI ACCTGC 1 cut(s) 2539
CaiI CAGNNNCTG 4 cut(s) 56, 4106, 4726, 4994
CciI TCATGA 2 cut(s) 922, 1999
CfoI GCGC 9 cut(s) 86, 407, 436, 1293, 3989, 4008, 4563, 4572, 4600
Cfr10I RCCGGY 1 cut(s) 4008
Cfr13I GGNCC 8 cut(s) 297, 2105, 2222, 3139, 3982, 4053, 4162, 4666
ClaI ATCGAT 3 cut(s) 255, 2763, 4074
CseI GACGC 4 cut(s) 2414, 2459, 3656, 4196
CsiI ACCWGGT 2 cut(s) 2809, 4540
DraI TTTAAA 2 cut(s) 1824, 5043
DraIII CACNNNGTG 2 cut(s) 2067, 2741
EaeI YGGCCR 3 cut(s) 3121, 3226, 4651
Eam1104I CTCTTC 6 cut(s) 699, 753, 1098, 2598, 3494, 4853
EarI CTCTTC 6 cut(s) 699, 753, 1098, 2598, 3494, 4853
EciI GGCGGA 2 cut(s) 624, 4164
Eco105I TACGTA 1 cut(s) 3894
Eco130I CCWWGG 2 cut(s) 2702, 3316
Eco24I GRGCYC 1 cut(s) 2637
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 2584
Eco47I GGWCC 7 cut(s) 297, 2105, 2222, 3982, 4053, 4162, 4666
Eco57I CTGAAG 7 cut(s) 734, 1006, 1775, 1878, 3744, 4834, 5112
Eco72I CACGTG 1 cut(s) 2456
Eco88I CYCGRG 2 cut(s) 87, 114
EcoO109I RGGNCCY 1 cut(s) 4162
EcoRI GAATTC 2 cut(s) 524, 1992
EcoRII CCWGG 9 cut(s) 616, 1102, 1493, 2809, 3135, 3806, 3967, 4540, 4987
EcoRV GATATC 1 cut(s) 2584
EcoT14I CCWWGG 2 cut(s) 2702, 3316
EcoT22I ATGCAT 3 cut(s) 475, 2886, 2955
EcoT38I GRGCYC 1 cut(s) 2637
ErhI CCWWGG 2 cut(s) 2702, 3316
Esp3I CGTCTC 3 cut(s) 2511, 3496, 4115
FalI AAGNNNNNCTT 4 cut(s) 269, 301, 983, 1015
FaqI GGGAC 5 cut(s) 2208, 2449, 3573, 3714, 4292
FauI CCCGC 3 cut(s) 2291, 3029, 5006
FauNDI CATATG 1 cut(s) 3148
FbaI TGATCA 1 cut(s) 4923
FblI GTMKAC 4 cut(s) 2467, 3331, 4687, 4959
FriOI GRGCYC 1 cut(s) 2637
GlaI GCGC 9 cut(s) 85, 406, 435, 1292, 3988, 4007, 4562, 4571, 4599
GsaI CCCAGC 1 cut(s) 4788
GsuI CTGGAG 3 cut(s) 774, 1516, 3790
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 6 cut(s) 616, 840, 3123, 3140, 3228, 4653
HapII CCGG 4 cut(s) 1421, 2433, 4009, 4214
HgaI GACGC 4 cut(s) 2414, 2459, 3656, 4196
HhaI GCGC 9 cut(s) 86, 407, 436, 1293, 3989, 4008, 4563, 4572, 4600
Hin1I GRCGYC 1 cut(s) 2656
Hin6I GCGC 9 cut(s) 84, 405, 434, 1291, 3987, 4006, 4561, 4570, 4598
HinP1I GCGC 9 cut(s) 84, 405, 434, 1291, 3987, 4006, 4561, 4570, 4598
HincII GTYRAC 6 cut(s) 687, 1418, 2468, 2924, 2970, 3671
HindII GTYRAC 6 cut(s) 687, 1418, 2468, 2924, 2970, 3671
HindIII AAGCTT 1 cut(s) 1763
HpaII CCGG 4 cut(s) 1421, 2433, 4009, 4214
HphI GGTGA 4 cut(s) 2039, 2380, 4530, 4643
Hpy99I CGWCG 4 cut(s) 2216, 3430, 4089, 4577
HpyCH4III ACNGT 6 cut(s) 779, 2194, 2819, 2967, 3495, 3772
Hsp92I GRCGYC 1 cut(s) 2656
HspAI GCGC 9 cut(s) 84, 405, 434, 1291, 3987, 4006, 4561, 4570, 4598
Kpn2I TCCGGA 1 cut(s) 4213
KpnI GGTACC 1 cut(s) 2531
KroI GCCGGC 1 cut(s) 4008
KroNI GCCGGC 1 cut(s) 4010
Ksp22I TGATCA 1 cut(s) 4923
LguI GCTCTTC 3 cut(s) 753, 2598, 4853
MabI ACCWGGT 2 cut(s) 2809, 4540
MfeI CAATTG 4 cut(s) 1843, 3015, 3117, 4328
MflI RGATCY 3 cut(s) 928, 1099, 4251
MhlI GDGCHC 6 cut(s) 1339, 1502, 2063, 2637, 3490, 4816
MlsI TGGCCA 3 cut(s) 3123, 3228, 4653
MluNI TGGCCA 3 cut(s) 3123, 3228, 4653
MlyI GAGTC 9 cut(s) 705, 2265, 2270, 2965, 3347, 3457, 4517, 4693, 4769
MmeI TCCRAC 4 cut(s) 229, 837, 882, 4912
Mox20I TGGCCA 3 cut(s) 3123, 3228, 4653
Mph1103I ATGCAT 3 cut(s) 475, 2886, 2955
MroI TCCGGA 1 cut(s) 4213
MroNI GCCGGC 1 cut(s) 4008
MroXI GAANNNNTTC 6 cut(s) 177, 199, 524, 747, 1092, 2166
MscI TGGCCA 3 cut(s) 3123, 3228, 4653
MslI CAYNNNNRTG 1 cut(s) 4233
Msp20I TGGCCA 3 cut(s) 3123, 3228, 4653
MspA1I CMGCKG 3 cut(s) 16, 2317, 5099
MspCI CTTAAG 2 cut(s) 1363, 1439
MspI CCGG 4 cut(s) 1421, 2433, 4009, 4214
MunI CAATTG 4 cut(s) 1843, 3015, 3117, 4328
Mva1269I GAATGC 5 cut(s) 543, 770, 2377, 2884, 4740
MvaI CCWGG 9 cut(s) 618, 1104, 1495, 2811, 3137, 3808, 3969, 4542, 4989
MvnI CGCG 5 cut(s) 407, 436, 3987, 4563, 4572
NaeI GCCGGC 1 cut(s) 4010
NciI CCSGG 1 cut(s) 2434
NcoI CCATGG 1 cut(s) 2702
NdeI CATATG 1 cut(s) 3148
NgoMIV GCCGGC 1 cut(s) 4008
NheI GCTAGC 3 cut(s) 109, 233, 3913
NlaIV GGNNCC 4 cut(s) 2224, 2529, 3041, 3805
NmeAIII GCCGAG 3 cut(s) 85, 4125, 4581
NmuCI GTSAC 6 cut(s) 1565, 2262, 2741, 2819, 3785, 4382
NsiI ATGCAT 3 cut(s) 475, 2886, 2955
NspI RCATGY 6 cut(s) 788, 1017, 2942, 3438, 3563, 3999
PagI TCATGA 2 cut(s) 922, 1999
PciI ACATGT 3 cut(s) 2938, 3559, 3995
PciSI GCTCTTC 3 cut(s) 753, 2598, 4853
PcsI WCGNNNNNNNCGW 3 cut(s) 646, 3658, 4581
PctI GAATGC 5 cut(s) 543, 770, 2377, 2884, 4740
PdiI GCCGGC 1 cut(s) 4010
PdmI GAANNNNTTC 6 cut(s) 177, 199, 524, 747, 1092, 2166
PflMI CCANNNNNTGG 1 cut(s) 4271
PleI GAGTC 9 cut(s) 704, 2265, 2269, 2965, 3346, 3457, 4516, 4692, 4768
PmaCI CACGTG 1 cut(s) 2456
PmlI CACGTG 1 cut(s) 2456
PpsI GAGTC 9 cut(s) 704, 2265, 2269, 2965, 3346, 3457, 4516, 4692, 4768
Ppu21I YACGTR 3 cut(s) 2130, 2456, 3894
PpuMI RGGWCCY 1 cut(s) 4162
PscI ACATGT 3 cut(s) 2938, 3559, 3995
PshBI ATTAAT 1 cut(s) 2337
PsiI TTATAA 2 cut(s) 659, 801
Psp5II RGGWCCY 1 cut(s) 4162
Psp6I CCWGG 9 cut(s) 616, 1102, 1493, 2809, 3135, 3806, 3967, 4540, 4987
PspCI CACGTG 1 cut(s) 2456
PspFI CCCAGC 1 cut(s) 4784
PspGI CCWGG 9 cut(s) 616, 1102, 1493, 2809, 3135, 3806, 3967, 4540, 4987
PspN4I GGNNCC 4 cut(s) 2224, 2529, 3041, 3805
PspPI GGNCC 8 cut(s) 297, 2105, 2222, 3139, 3982, 4053, 4162, 4666
PspPPI RGGWCCY 1 cut(s) 4162
PstI CTGCAG 4 cut(s) 38, 97, 4731, 4836
PstNI CAGNNNCTG 4 cut(s) 56, 4106, 4726, 4994
PsuI RGATCY 3 cut(s) 928, 1099, 4251
PvuII CAGCTG 3 cut(s) 16, 2317, 5099
RseI CAYNNNNRTG 1 cut(s) 4233
SalI GTCGAC 1 cut(s) 2466
SapI GCTCTTC 3 cut(s) 753, 2598, 4853
Sau96I GGNCC 8 cut(s) 297, 2105, 2222, 3139, 3982, 4053, 4162, 4666
SchI GAGTC 9 cut(s) 705, 2265, 2270, 2965, 3347, 3457, 4517, 4693, 4769
SduI GDGCHC 6 cut(s) 1339, 1502, 2063, 2637, 3490, 4816
SexAI ACCWGGT 2 cut(s) 2809, 4540
SfcI CTRYAG 5 cut(s) 34, 93, 3481, 4727, 4832
SinI GGWCC 7 cut(s) 297, 2105, 2222, 3982, 4053, 4162, 4666
SmiMI CAYNNNNRTG 1 cut(s) 4233
SmlI CTYRAG 6 cut(s) 1363, 1439, 1702, 3790, 3973, 4338
SmoI CTYRAG 6 cut(s) 1363, 1439, 1702, 3790, 3973, 4338
SnaBI TACGTA 1 cut(s) 3894
SpeI ACTAGT 3 cut(s) 4750, 4822, 4973
SspI AATATT 4 cut(s) 244, 1604, 3291, 3527
StyI CCWWGG 2 cut(s) 2702, 3316
TaaI ACNGT 6 cut(s) 779, 2194, 2819, 2967, 3495, 3772
TatI WGTACW 5 cut(s) 1189, 2932, 3095, 3747, 4093
TauI GCSGC 2 cut(s) 1458, 4593
TseFI GTSAC 6 cut(s) 1565, 2262, 2741, 2819, 3785, 4382
Tsp45I GTSAC 6 cut(s) 1565, 2262, 2741, 2819, 3785, 4382
TspGWI ACGGA 6 cut(s) 496, 1514, 2962, 3863, 4073, 4338
Van91I CCANNNNNTGG 1 cut(s) 4271
Vha464I CTTAAG 2 cut(s) 1363, 1439
VneI GTGCAC 2 cut(s) 2059, 4812
VpaK11BI GGWCC 7 cut(s) 297, 2105, 2222, 3982, 4053, 4162, 4666
VspI ATTAAT 1 cut(s) 2337
XceI RCATGY 6 cut(s) 788, 1017, 2942, 3438, 3563, 3999
XcmI CCANNNNNNNNNTGG 2 cut(s) 424, 4924
XmiI GTMKAC 4 cut(s) 2467, 3331, 4687, 4959
XmnI GAANNNNTTC 6 cut(s) 177, 199, 524, 747, 1092, 2166
ZraI GACGTC 1 cut(s) 2657
Zsp2I ATGCAT 3 cut(s) 475, 2886, 2955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.