FvH4_1g16630

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000307
Physical Location & Seq
Reverse (-)
9608303 .. 9615156
6854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16630.t1

Sequence Viewer

Length: 5964 bp
ATGGAAGTAGCTGCTTCATCGTCTTCAACATCTAGTCCACCCTCTAATTGGGAGCATGATGTGTTCCTCAGTTTCAGGGGCAAGGACACCCGCCATGGTTTCACAGACCATTTATACTTTGCTCTGAATCAGAGAGGAATTGACACATATAGGGATGCAGAGAAACTTCCAAGGGGAAAATACATGTCTTTAGAACTTGTGGAAGCAATTCAGGAGTCAAGGTTTGCCGTCATTGTTCTTTCAGCTAACTATGCCACTTCAAGTTGGTGTTTAGATGAGCTTGCTCATATTCTTGAATGCAAGAAAGTGAGGGGACTGGAAGTTCTTCCGGTTTTTTACCATGTAGAGCCATCCGAGATAAGGAAGCAAGCTGGAAGGTTGGGAATGGAGATCGCCAAGCATGAGACAGATTTCTTGGAGAAGAGGAGGAAGGTGGACACGTGGAAGGAAGCTTTGAAGGAGATAGCCAGTCTCTCCGGATGGCATGTCACCAAGGACAGGGGAGAATCAGAAGTTATACAGGAAATAGTTAAAGGGATTTCAGACATGTTGAACAACATGTTATGCACCCCGGACAGAGATTTAATTGGGATGGATGCACGTATTGAGAAAATGGAGTGGTACCTGGATCTAAGGTCGAGTGATGTTCTTGCTGTAGGGATTTGGGGGATGGGGGGAATTGGTAAGACAACTCTGGCAAAAGAAGTTTTCAAAAAGATTCGTAACCAATTTGATATTAGCGGTTTTGTTTCCGACGTGAGATTGTATTCAGAAGAAAAGGCTCTAGTTGAGTTGCAGAAACTTCTTTGTGGTTCCTTCTTCGGGGACAGCAATATAAATATAGACACTGTCGAAAGAGGGATCAGGTTATTGAAGAAGGTACTATGCAAGAAAAAGGTGCTTATCGTACTAGATGATGTTGACAAACTTAAACAACTGAAAGATTTAACACCTGGAATGCATAATGAAGAGAATTCTTGGGCGCCAGGGAGTCGACTCATTATAACAACTAGAGATAGGCGCATATTGACAGAATGTGGAGTACTGGAGTCTAAAATATATGAGGTTGAAAAACTGAGTAATGTAGAAGGATTTCAGGTCATATGTCAACAAGCCTTCAAGAAAAACTATCCACCCGTTGAGTTTGTAGAGCTGTCCAAGAGTTTCGTAAAATATGCCAGTGGCCTTCCTTTAGCTCATAAAGTTCTAGGATCACACTTGTATGGTAGGGAGATAGATGAATGGTTAGAGGTATTGTATAGACTGGACGAAGAGCTGGATAAAGATATTTTTAGTGTGCTCCAAATAAGTTTTGATGGATTACATGAGACTGATAAGAAAATCTTTTTGGACATTGCATGTTTCTTCAATGGTGAGGATCATGTTCGTGTAAAAAATATATTGAAAGGTTGTGGCTTTTCTTCCGGAATAGGCATAACTAACCTTATCAATAAATCTTTGATTAAGATTGAAAGGAAGAAACTGTGGATGCATGATTTACTGCGGTGCTTGGGTTGGCATATTGTCCGTAGAGAATCTGTTGTCCCAGGTAAACGTAGCAGGTTGTGGCTTGATGGCAATTCATTTGACTACAAACGCAGAAGGTTATGGCATGTGGAGGATGCTCGCAATGGAACAACTGCTGTTGAAGGCTTGTTCTTAAGCTTGCATGAAAAAGAAGAAATACGTTTGAATGAAGACCCGTTCTTAACTATGCACAACCTAAGATTGTTGAAGATTTGTAATGTGAACTTTCATGATGTGCAGTTCACCTATCTTTCTAGAAAATTACGGTTTTTGGAATGGCATGAATGCCCACTAGAATCTTTGTCATCTTGCTTTACACCGGATTCACTAGTAAGCTTGGTTGAATTAAAGATTCCTAACAGCCGCATTGAACAAGTATGGAATGGAAGGATATCTCTGGAAATGCTAATACTCATGGATCTTTCCAACTGCCAATATTTAACGTTGACCCCGGACTTCAGTACGGTCCCGAATCTTGAGCGGTTAATCCTCCAAGGTTGTAAGAAGTTATCAGAGGTTCACCATACAATCGGGAATCTTCAGAAACTGGGTCTTTTGAACTTGAAAGGCTGCACAAGTCTAGAGAGTCTCCCCCAATATATTGGCTTGTCATCTCTCCAAACTTTTATTCTGTCAGGATGTTCAAAACTCCAAGAGTTTCCGCAGATTTTGGAGAACATGGATACATTATCAGAACTTTATCTAGATGGCACGGCTATTTGGGAACTACCTGAATCAATCCAGCATTTGAAAGGCCTTGTTTGGCTAAATCTAAGCGGCTGCACGAACCTTCTCAGTATTCCAAGCATCATTTGTAGTAGTTTGACATCCCTGAAATTTCTCTATCTGTCACTGTGCTCAAGTATGGACAATCTGCCGGATAACATAGGCTCCTTGGAACACTTGGAGGAGCTCGACGCATGTAATACTGCTATAAGAAAAGTGCCCGAGTCCACATCTCACCTCAAGAATCTTAAACTATTGTGTTTTCATGGCTGCTCTGGATTCACAGGTTTAGAATTGCCAAACAAGTTCTCTGGTTTGAGGTCTTTGACAACACTAAACCTAGGTAGATGTAATCTCAGAGAAGGAGCAATCCCTGATGACATTGGTTATTTATTCTCATTGCAGAGTCTTGATTTAAGTGAGAACGACTTTTTCACCATACCTGAAAGCATCTCCCAGCTTTCTGAGCTTTCAGAAATTTCTCTGTTTAGGTGTAGCAAACTACAGTTGTTGCCAAAAGATCTTCCGTCAAGTCTAAGAAATGTAAATGTACGTGGTTGTCCTATGCTGACAAATTCTTCGTCGAGTTGGAGGAGATATCCTCCTCAAAAGGGTTTGAGTATCATAAACTGTCGGAAACCGGAGGAGGAGGAGGAGGAGGAAATCTTTCCTGAACTCCATAAGTTTCCTTTAGGAAATCTGGAAGTACTCGATCTTTCCTACTGCCAACACTTGAAGAAGGTCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAACTGAACCTTGAAGGTTGTGAAAAGCTATCTGAGGTTCACCCTACAGTTGGGCGTCTCCAACATCTGATTGTTCTGAATATGAAAGGATGTGTTGATCTATTGAAATTTCCCCGTTCCATCAGCTTGAAGTCTCTAAAATTATTTGATCTTTCAGGATGTTCAAAACTGAAAGAGTTTCCAGAGATTAATGGAGATATGGGGAAATTTTCACAACTGCATTTAGATGGGACGGCATTAAAGTATCTGCCAATACCAATGAAGCAATTCACATGCCCTATTTTGATAACTTTAAGAGGCTGCAGGAACCTTTTGACTGTTCCGGTCCTGTTAAGTCTGAATTCTCTCAATCTGTCAGGCTGTTCCCGTATAACTAGACTGCCAGCCGACCTGGGAACAATGGTACATTTGGAGGAGCTTGATGCCTCTGAAACTTCCATAACTGAAGTACCGTATTCCATTTTATGCTTGGAGAAGCTCAAAGTGTTATCTTTCTGTGGATGTTTTGGTTTGCAGTTACCTGACTGGTTCTCGAATTTAAAATCTTTGAGATCATTAAATCTACGTAGGTGTGGTCTACCAAAAAATTTTCATGATAGGCTCTTCTGGTCGACTTCACTGCAGAGTCTGGATTTGGGTGAAAATGATTTGGTGAGTATACCTAATGAACTTGGTAGACTATCCTTTTTGCAGAAATTGGATTTCAGTGGAAACAATTTAGTCAGTGTACCAAATGAAATTGGTTGCCTATCCTCTTTGCAGTGTTTAGATTTGAGTGAAAACAACTTTGTGAGTATACCTGAAAGCATCTCTCAACTTTCTTGGCTTACAGAACTTCACTTGTTTAGGTGTAGTGTGTTACAGTCTTTGCCAAGAAATCTTCCATTCAATCTAAAATATGTCAATGCACGAGAATGTCCCATGTTGAAGAATAATGCCGATACTTTGACAATGTGGGCTTCGGGGAAACGGTTCTGCTTCATAAATTGTGGACAATCAGAAGAGGATAATGTTCAGGCAAGCCACGTTCCAGTTCCAGAAGACAGTATTGGACTACTCTTTTCCAAATACATTCAGAATCGAATCTATGGTAAAAAACCATTTGAATTTCGTTTTCCACATAGTACAAGAATCCCCCATTTGTGGAGCCATTGGAGGAGTGGCCCTTCTGTGGCAGTCCCGCTATCTGATAGTAATAGTTCGTGGATTGGATTTGCTCTATTTGTTGTTTTCGAAATCCTTGAGAAAGAAGATTTCGACAGCATTTGGGAATCAGAGGAGACTATTTGTGAATTTCATACCAATTTTGGGTGTGAAAATTCACTAGTTTTCCAAAATTTCCCTGACTTCAGGACTGGTCCATATGGACTTTGTTGCTACGAACCACGAGTTGGGAAATTTGGTGGCTTGTTCGATAAATTAGTACTTCAAGCTACAGTTTCAACAAAGAGACCAGATTTGAAAGTGAGAGGTTGTGGGTTGCATCTAATATCAGAGCACAATGCTGCAGAGTTTGTTCAAAGTTTAATAAACCAGACTGCTATTCAGCACCTCGATTTCAATTTTGATCGACATTGTGAGGAAATATTAGAAAAGGCAACGACTTGTGACCTGATAGATGTAGACCCTGACGATCTATGGGAATTAGATTCTACATCAACTATGAATACAGATAACTGCGACATGAGTAGCTGCAGTATCCAAATCAGAAAACAGCTCTCATTCCTCTATGAGGGACGCAATGGATGTGAGAAGAGTTTCCATGTTTGTCTTCCTGATTCATCAGTTGTACTGACATCTCCGACCTGGTTCATCAAGCGCCACGCAGGTGATGTAACACTATGTTACATCAGTGAAAACTTACATGCTAATCAGAGATGGGTGGGATTGGAGCTATTTGTTATATTTGGCCGGCGGACACGTGCTTCAAGTGATAACCATAATAACAGTTTCGCCTTTCATATTGAGTTGTGTGGTCATGATAATGAAAGTCTGATAATGCATGAATTCCTCAAGAGACATAGTTGTCTTGGAGAGACGAATCAACTTGTGGTATTACATTTACCACGTGTTTATTTTCAACAACAGTTGAATCAATGTCAGGTTATTAGCGCATTGTTTACAACCATTAACCCAGAATTGGAGGTTGTAATGTGTGGGAGCCGTCTAGTTTATCAGCAAGATTTACAAGACTTGATCTGTTCATTGACTGCGGCTGCAAGAAAGTTTGGGGAACATGTCCTTACTCAACTTTGTTCCCTGGCCTGCACTCAAACTGATGATCAGATCATTGCAGTAGAAGGAGAAATGCCCATCAATTGTGATTCCTCTTCTCAAAGAAGTAAAGCACAAGTACCAGAACAAGTTTTAACTTCAATGATGAGGCTGAAAAGTTATAGATGCTATCTCCGGCAAAAGCAAGTTTTTGTAAATTCTGAACCTACCCCATTAGTTCACTCCGGATCAGTAATACCAGTCCATGCCAAATGCTGTCTTTTCGAATGCAGTGAGAGGAACTTTGGAAATGAAGCAGAAGAAAACACAAAGTCGTCATGTCATCATGTACGTATAATGGCTGAAACTCAACTGTACGGTAGCTATAGTTCATTTCAGCTGAAAACATCCCTCCAGTTGTTACTTAAACACTCCAAGGTGGCCACTCTCAGTCTTGGTGGACATACAATTTCTGCTTTCAAGAAGTTTGATCCATCCTCCGCATCCAATATTATTTGTTTCTCTGATAAGGAAATATTCCAGTGTGGTTCAGAAATGAGATGTCGTATCAGATGTCTTCTAGGTCCAGGGTGGGAATCAAACTACCTCCAAGATTGCTTGAGGATAGGAATTGGAGAGGACTTGGTGTATGTGTTGCCTTTGAAGTTCATGACCAGATTGCAACTACCTCCCCAGGGCCGGTCAAACTTCTCTGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

1988

Amino Acids

225.42

Weight (kDa)

6.14

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 189 5.5e-51 TIR domain
TIR_2 PF13676 21 - 117 1.2e-14 TIR domain
NB-ARC PF00931 212 - 376 3.9e-21 NB-ARC domain
NACHT PF05729 221 - 370 6e-06 NACHT domain
WHD_ROQ1 PF23282 443 - 513 4.2e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 658 - 762 7.2e-06 Leucine-rich repeat region
LRR_13 PF23286 712 - 779 4.6e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 765 - 867 1.4e-11 Leucine-rich repeat region
LRR_14 PF23598 1111 - 1218 7.2e-11 Leucine-rich repeat region
LRR_14 PF23598 1192 - 1293 5.5e-11 Leucine-rich repeat region
LRR_8 PF13855 1215 - 1272 1.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1004
AarI CACCTGC 1 cut(s) 4844
AasI GACNNNNNNGTC 1 cut(s) 5052
Acc36I ACCTGC 2 cut(s) 1551, 4844
Acc65I GGTACC 1 cut(s) 621
AccB1I GGYRCC 2 cut(s) 621, 982
AccB7I CCANNNNNTGG 1 cut(s) 4418
AccBSI CCGCTC 1 cut(s) 2008
AccI GTMKAC 7 cut(s) 994, 3604, 3638, 3685, 3703, 3823, 4650
AccIII TCCGGA 3 cut(s) 476, 1424, 5486
AclI AACGTT 1 cut(s) 1970
AclWI GGATC 7 cut(s) 636, 869, 1219, 1386, 1953, 5497, 5726
AcoI YGGCCR 2 cut(s) 4936, 5682
AcuI CTGAAG 4 cut(s) 1969, 2051, 3492, 4360
AcvI CACGTG 3 cut(s) 441, 4949, 5096
AcyI GRCGYC 2 cut(s) 983, 3082
AflII CTTAAG 2 cut(s) 1660, 3023
AflIII ACRYGT 7 cut(s) 183, 438, 546, 558, 4946, 5095, 5263
AhlI ACTAGT 2 cut(s) 1855, 4351
AjiI CACGTC 1 cut(s) 757
AjnI CCWGG 9 cut(s) 624, 952, 985, 1546, 3417, 4832, 5286, 5827, 5934
AjuI GAANNNNNNNTTGG 8 cut(s) 1331, 1363, 2139, 2171, 4059, 4091, 4401, 4433
AleI CACNNNNGTG 1 cut(s) 4854
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
Alw21I GWGCWC 4 cut(s) 1302, 2387, 2442, 4527
AlwI GGATC 7 cut(s) 636, 869, 1219, 1386, 1953, 5497, 5726
AlwNI CAGNNNCTG 2 cut(s) 2074, 3655
Ama87I CYCGRG 1 cut(s) 2474
Aor13HI TCCGGA 3 cut(s) 476, 1424, 5486
AoxI GGCC 7 cut(s) 1183, 2281, 4189, 4936, 5289, 5682, 5938
ApeKI GCWGC 8 cut(s) 11, 2097, 2307, 2523, 3327, 4532, 4719, 5243
ArsI GACNNNNNNTTYG 4 cut(s) 2815, 2819, 2847, 2851
AseI ATTAAT 1 cut(s) 3216
Asp700I GAANNNNTTC 7 cut(s) 207, 324, 1092, 2919, 3293, 3998, 4784
Asp718I GGTACC 1 cut(s) 621
AspA2I CCTAGG 1 cut(s) 2593
AspLEI GCGC 4 cut(s) 985, 1023, 4848, 5141
AspS9I GGNCC 7 cut(s) 1993, 2995, 3352, 4190, 4385, 5825, 5938
AsuC2I CCSGG 2 cut(s) 572, 1979
AsuII TTCGAA 2 cut(s) 4260, 5526
AvaI CYCGRG 1 cut(s) 2474
AvaII GGWCC 5 cut(s) 1993, 2995, 3352, 4385, 5825
AvrII CCTAGG 1 cut(s) 2593
BaeGI GKGCMC 1 cut(s) 2475
BaeI ACNNNNGTAYC 2 cut(s) 3459, 3492
BalI TGGCCA 1 cut(s) 5684
BanI GGYRCC 2 cut(s) 621, 982
BanII GRGCYC 1 cut(s) 2442
BauI CACGAG 2 cut(s) 3936, 4413
BbrPI CACGTG 3 cut(s) 441, 4949, 5096
BbsI GAAGAC 5 cut(s) 15, 1704, 4074, 4790, 5810
Bbv12I GWGCWC 4 cut(s) 1302, 2387, 2442, 4527
BbvI GCAGC 7 cut(s) 2084, 2294, 2510, 3314, 4519, 4706, 5230
BceAI ACGGC 4 cut(s) 212, 2256, 3276, 5175
BcgI CGANNNNNNTGC 2 cut(s) 3628, 3662
BciT130I CCWGG 9 cut(s) 626, 954, 987, 1548, 3419, 4834, 5288, 5829, 5936
BciVI GTATCC 2 cut(s) 2203, 4736
BclI TGATCA 1 cut(s) 5308
BcnI CCSGG 2 cut(s) 572, 1979
BcuI ACTAGT 2 cut(s) 1855, 4351
BfmI CTRYAG 9 cut(s) 654, 2756, 3072, 3328, 3647, 4461, 4533, 4720, 5626
BfoI RGCGCY 2 cut(s) 986, 4849
BfrI CTTAAG 2 cut(s) 1660, 3023
BfuAI ACCTGC 2 cut(s) 1551, 4844
BfuI GTATCC 2 cut(s) 2203, 4736
BglII AGATCT 1 cut(s) 2773
BlnI CCTAGG 1 cut(s) 2593
BmcAI AGTACT 3 cut(s) 1044, 2961, 4452
Bme18I GGWCC 5 cut(s) 1993, 2995, 3352, 4385, 5825
BmeT110I CYCGRG 1 cut(s) 2474
BmgBI CACGTC 1 cut(s) 757
BmgT120I GGNCC 7 cut(s) 1993, 2995, 3352, 4190, 4385, 5825, 5938
BmrI ACTGGG 1 cut(s) 2084
BmuI ACTGGG 1 cut(s) 2084
BpiI GAAGAC 5 cut(s) 15, 1704, 4074, 4790, 5810
BplI GAGNNNNNCTC 2 cut(s) 2839, 2871
BpmI CTGGAG 2 cut(s) 1067, 5639
Bpu14I TTCGAA 2 cut(s) 4260, 5526
BpuEI CTTGAG 6 cut(s) 2024, 2371, 2477, 4289, 5024, 5881
BpuMI CCSGG 2 cut(s) 572, 1979
BsaAI YACGTR 7 cut(s) 441, 602, 2807, 3593, 4949, 5096, 5594
BsaHI GRCGYC 2 cut(s) 983, 3082
BsaI GGTCTC 1 cut(s) 4471
BsaWI WCCGGW 7 cut(s) 328, 476, 1424, 1846, 2893, 3349, 5486
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 2402, 2432
Bse118I RCCGGY 2 cut(s) 4938, 5940
Bse3DI GCAATG 5 cut(s) 1353, 1636, 2651, 4774, 5316
BseAI TCCGGA 3 cut(s) 476, 1424, 5486
BseBI CCWGG 9 cut(s) 626, 954, 987, 1548, 3419, 4834, 5288, 5829, 5936
BseMI GCAATG 5 cut(s) 1353, 1636, 2651, 4774, 5316
BseMII CTCAG 7 cut(s) 82, 1067, 2335, 2623, 2709, 3051, 5704
BseSI GKGCMC 1 cut(s) 2475
BseXI GCAGC 7 cut(s) 2084, 2294, 2510, 3314, 4519, 4706, 5230
BseYI CCCAGC 1 cut(s) 2709
BsgI GTGCAG 4 cut(s) 1784, 2083, 2293, 5278
BshFI GGCC 7 cut(s) 1185, 2283, 4191, 4938, 5291, 5684, 5940
BshNI GGYRCC 2 cut(s) 621, 982
BsiHKAI GWGCWC 4 cut(s) 1302, 2387, 2442, 4527
BsiHKCI CYCGRG 1 cut(s) 2474
BslFI GGGAC 9 cut(s) 327, 839, 1529, 1979, 2981, 3271, 3930, 4190, 4776
BsmBI CGTCTC 2 cut(s) 3089, 5057
BsmFI GGGAC 9 cut(s) 327, 839, 1529, 1979, 2981, 3271, 3930, 4190, 4776
BsmI GAATGC 4 cut(s) 302, 963, 1817, 5534
BsnI GGCC 7 cut(s) 1185, 2283, 4191, 4938, 5291, 5684, 5940
Bso31I GGTCTC 1 cut(s) 4471
BsoBI CYCGRG 1 cut(s) 2474
Bsp119I TTCGAA 2 cut(s) 4260, 5526
Bsp1286I GDGCHC 5 cut(s) 1302, 2387, 2442, 2475, 4527
Bsp13I TCCGGA 3 cut(s) 476, 1424, 5486
Bsp19I CCATGG 1 cut(s) 94
BspANI GGCC 7 cut(s) 1185, 2283, 4191, 4938, 5291, 5684, 5940
BspCNI CTCAG 7 cut(s) 81, 1068, 2334, 2622, 2710, 3052, 5703
BspEI TCCGGA 3 cut(s) 476, 1424, 5486
BspHI TCATGA 4 cut(s) 1756, 3619, 5005, 5910
BspMAI CTGCAG 4 cut(s) 3332, 3651, 4537, 4724
BspMI ACCTGC 2 cut(s) 1551, 4844
BspPI GGATC 7 cut(s) 636, 869, 1219, 1386, 1953, 5497, 5726
BspQI GCTCTTC 2 cut(s) 1266, 3635
BspT104I TTCGAA 2 cut(s) 4260, 5526
BspT107I GGYRCC 2 cut(s) 621, 982
BspTI CTTAAG 2 cut(s) 1660, 3023
BspTNI GGTCTC 1 cut(s) 4471
BsrBI CCGCTC 1 cut(s) 2008
BsrDI GCAATG 5 cut(s) 1353, 1636, 2651, 4774, 5316
BsrFI RCCGGY 2 cut(s) 4938, 5940
BssAI RCCGGY 2 cut(s) 4938, 5940
BssNAI GTATAC 2 cut(s) 3686, 3824
BssNI GRCGYC 2 cut(s) 983, 3082
BssSI CACGAG 2 cut(s) 3936, 4413
BssT1I CCWWGG 7 cut(s) 94, 170, 492, 2020, 2421, 2593, 5676
Bst1107I GTATAC 2 cut(s) 3686, 3824
Bst2BI CACGAG 2 cut(s) 3936, 4413
Bst2UI CCWGG 9 cut(s) 626, 954, 987, 1548, 3419, 4834, 5288, 5829, 5936
Bst6I CTCTTC 7 cut(s) 416, 963, 1266, 3635, 4023, 4775, 5362
BstACI GRCGYC 2 cut(s) 983, 3082
BstAFI CTTAAG 2 cut(s) 1660, 3023
BstBAI YACGTR 7 cut(s) 441, 602, 2807, 3593, 4949, 5096, 5594
BstBI TTCGAA 2 cut(s) 4260, 5526
BstC8I GCNNGC 8 cut(s) 282, 369, 1627, 1667, 3411, 4048, 4940, 5293
BstDSI CCRYGG 1 cut(s) 94
BstENI CCTNNNNNAGG 1 cut(s) 4757
BstH2I RGCGCY 2 cut(s) 986, 4849
BstHHI GCGC 4 cut(s) 985, 1023, 4848, 5141
BstMWI GCNNNNNNNGC 2 cut(s) 251, 2719
BstNI CCWGG 9 cut(s) 626, 954, 987, 1548, 3419, 4834, 5288, 5829, 5936
BstSFI CTRYAG 9 cut(s) 654, 2756, 3072, 3328, 3647, 4461, 4533, 4720, 5626
BstSLI GKGCMC 1 cut(s) 2475
BstSNI TACGTA 2 cut(s) 3593, 5594
BstV1I GCAGC 7 cut(s) 2084, 2294, 2510, 3314, 4519, 4706, 5230
BstV2I GAAGAC 5 cut(s) 15, 1704, 4074, 4790, 5810
BstX2I RGATCY 3 cut(s) 628, 1945, 2773
BstXI CCANNNNNNTGG 1 cut(s) 2129
BstYI RGATCY 3 cut(s) 628, 1945, 2773
BstZ17I GTATAC 2 cut(s) 3686, 3824
BsuI GTATCC 2 cut(s) 2203, 4736
BsuRI GGCC 7 cut(s) 1185, 2283, 4191, 4938, 5291, 5684, 5940
BtgI CCRYGG 1 cut(s) 94
BtrI CACGTC 1 cut(s) 757
BtsI GCAGTG 3 cut(s) 3644, 3794, 5539
BveI ACCTGC 2 cut(s) 1551, 4844
Cac8I GCNNGC 8 cut(s) 282, 369, 1627, 1667, 3411, 4048, 4940, 5293
CaiI CAGNNNCTG 2 cut(s) 2074, 3655
CciI TCATGA 4 cut(s) 1756, 3619, 5005, 5910
CfoI GCGC 4 cut(s) 985, 1023, 4848, 5141
Cfr10I RCCGGY 2 cut(s) 4938, 5940
Cfr13I GGNCC 7 cut(s) 1993, 2995, 3352, 4190, 4385, 5825, 5938
CseI GACGC 3 cut(s) 2453, 3071, 4773
CsiI ACCWGGT 1 cut(s) 4832
DinI GGCGCC 1 cut(s) 984
DraI TTTAAA 1 cut(s) 3567
DrdI GACNNNNNNGTC 1 cut(s) 5052
DseDI GACNNNNNNGTC 1 cut(s) 5052
EaeI YGGCCR 2 cut(s) 4936, 5682
Eam1104I CTCTTC 7 cut(s) 416, 963, 1266, 3635, 4023, 4775, 5362
EarI CTCTTC 7 cut(s) 416, 963, 1266, 3635, 4023, 4775, 5362
EciI GGCGGA 1 cut(s) 4957
Ecl136II GAGCTC 1 cut(s) 2440
Eco105I TACGTA 2 cut(s) 3593, 5594
Eco130I CCWWGG 7 cut(s) 94, 170, 492, 2020, 2421, 2593, 5676
Eco147I AGGCCT 1 cut(s) 2283
Eco24I GRGCYC 1 cut(s) 2442
Eco31I GGTCTC 1 cut(s) 4471
Eco32I GATATC 2 cut(s) 1920, 2852
Eco47I GGWCC 5 cut(s) 1993, 2995, 3352, 4385, 5825
Eco53kI GAGCTC 1 cut(s) 2440
Eco57I CTGAAG 4 cut(s) 1969, 2051, 3492, 4360
Eco72I CACGTG 3 cut(s) 441, 4949, 5096
Eco88I CYCGRG 1 cut(s) 2474
EcoICRI GAGCTC 1 cut(s) 2440
EcoNI CCTNNNNNAGG 1 cut(s) 4757
EcoO109I RGGNCCY 1 cut(s) 2995
EcoRI GAATTC 3 cut(s) 973, 3367, 5033
EcoRII CCWGG 9 cut(s) 624, 952, 985, 1546, 3417, 4832, 5286, 5827, 5934
EcoRV GATATC 2 cut(s) 1920, 2852
EcoT14I CCWWGG 7 cut(s) 94, 170, 492, 2020, 2421, 2593, 5676
EcoT22I ATGCAT 3 cut(s) 963, 1494, 5031
EcoT38I GRGCYC 1 cut(s) 2442
EgeI GGCGCC 1 cut(s) 984
EheI GGCGCC 1 cut(s) 984
ErhI CCWWGG 7 cut(s) 94, 170, 492, 2020, 2421, 2593, 5676
Esp3I CGTCTC 2 cut(s) 3089, 5057
FalI AAGNNNNNCTT 2 cut(s) 1328, 1360
FaqI GGGAC 9 cut(s) 327, 839, 1529, 1979, 2981, 3271, 3930, 4190, 4776
FauI CCCGC 2 cut(s) 98, 4215
FauNDI CATATG 2 cut(s) 1103, 4390
FbaI TGATCA 1 cut(s) 5308
FblI GTMKAC 7 cut(s) 994, 3604, 3638, 3685, 3703, 3823, 4650
FriOI GRGCYC 1 cut(s) 2442
GlaI GCGC 4 cut(s) 984, 1022, 4847, 5140
GsaI CCCAGC 1 cut(s) 2713
GsuI CTGGAG 2 cut(s) 1067, 5639
HaeII RGCGCY 2 cut(s) 986, 4849
HaeIII GGCC 7 cut(s) 1185, 2283, 4191, 4938, 5291, 5684, 5940
HgaI GACGC 3 cut(s) 2453, 3071, 4773
HhaI GCGC 4 cut(s) 985, 1023, 4848, 5141
Hin1I GRCGYC 2 cut(s) 983, 3082
Hin6I GCGC 4 cut(s) 983, 1021, 4846, 5139
HinP1I GCGC 4 cut(s) 983, 1021, 4846, 5139
HincII GTYRAC 5 cut(s) 922, 995, 1109, 1974, 3639
HindII GTYRAC 5 cut(s) 922, 995, 1109, 1974, 3639
HindIII AAGCTT 3 cut(s) 450, 1663, 1861
Hpy99I CGWCG 3 cut(s) 758, 2447, 2839
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 2719
Hsp92I GRCGYC 2 cut(s) 983, 3082
HspAI GCGC 4 cut(s) 983, 1021, 4846, 5139
KasI GGCGCC 1 cut(s) 982
Kpn2I TCCGGA 3 cut(s) 476, 1424, 5486
KpnI GGTACC 1 cut(s) 625
KroI GCCGGC 1 cut(s) 4938
KroNI GCCGGC 1 cut(s) 4940
Ksp22I TGATCA 1 cut(s) 5308
LguI GCTCTTC 2 cut(s) 1266, 3635
LmnI GCTCC 9 cut(s) 52, 1305, 2423, 2437, 2618, 3442, 4173, 4918, 5187
Lsp1109I GCAGC 7 cut(s) 2084, 2294, 2510, 3314, 4519, 4706, 5230
MabI ACCWGGT 1 cut(s) 4832
MbiI CCGCTC 1 cut(s) 2008
MfeI CAATTG 1 cut(s) 5344
MflI RGATCY 3 cut(s) 628, 1945, 2773
MhlI GDGCHC 5 cut(s) 1302, 2387, 2442, 2475, 4527
MlsI TGGCCA 1 cut(s) 5684
MluNI TGGCCA 1 cut(s) 5684
Mly113I GGCGCC 1 cut(s) 983
MlyI GAGTC 8 cut(s) 224, 990, 1000, 1058, 2122, 2486, 2668, 3661
MmeI TCCRAC 6 cut(s) 777, 1977, 2822, 2867, 3112, 4853
Mox20I TGGCCA 1 cut(s) 5684
Mph1103I ATGCAT 3 cut(s) 963, 1494, 5031
MroI TCCGGA 3 cut(s) 476, 1424, 5486
MroNI GCCGGC 1 cut(s) 4938
MroXI GAANNNNTTC 7 cut(s) 207, 324, 1092, 2919, 3293, 3998, 4784
MscI TGGCCA 1 cut(s) 5684
MslI CAYNNNNRTG 6 cut(s) 1221, 1386, 3252, 3940, 4854, 5010
Msp20I TGGCCA 1 cut(s) 5684
MspA1I CMGCKG 1 cut(s) 5641
MspCI CTTAAG 2 cut(s) 1660, 3023
MunI CAATTG 1 cut(s) 5344
Mva1269I GAATGC 4 cut(s) 302, 963, 1817, 5534
MvaI CCWGG 9 cut(s) 626, 954, 987, 1548, 3419, 4834, 5288, 5829, 5936
MwoI GCNNNNNNNGC 2 cut(s) 251, 2719
NaeI GCCGGC 1 cut(s) 4940
NarI GGCGCC 1 cut(s) 983
NciI CCSGG 2 cut(s) 572, 1979
NcoI CCATGG 1 cut(s) 94
NdeI CATATG 2 cut(s) 1103, 4390
NgoMIV GCCGGC 1 cut(s) 4938
NmuCI GTSAC 4 cut(s) 487, 2376, 4634, 5956
NsiI ATGCAT 3 cut(s) 963, 1494, 5031
NspV TTCGAA 2 cut(s) 4260, 5526
OliI CACNNNNGTG 1 cut(s) 4854
PagI TCATGA 4 cut(s) 1756, 3619, 5005, 5910
PaqCI CACCTGC 1 cut(s) 4844
PasI CCCWGGG 1 cut(s) 5935
PceI AGGCCT 1 cut(s) 2283
PciI ACATGT 4 cut(s) 183, 546, 558, 5263
PciSI GCTCTTC 2 cut(s) 1266, 3635
PctI GAATGC 4 cut(s) 302, 963, 1817, 5534
PdiI GCCGGC 1 cut(s) 4940
PdmI GAANNNNTTC 7 cut(s) 207, 324, 1092, 2919, 3293, 3998, 4784
PflFI GACNNNGTC 1 cut(s) 848
PflMI CCANNNNNTGG 1 cut(s) 4418
PleI GAGTC 8 cut(s) 223, 990, 999, 1057, 2121, 2485, 2667, 3660
PluTI GGCGCC 1 cut(s) 986
PmaCI CACGTG 3 cut(s) 441, 4949, 5096
PmlI CACGTG 3 cut(s) 441, 4949, 5096
PpsI GAGTC 8 cut(s) 223, 990, 999, 1057, 2121, 2485, 2667, 3660
Ppu21I YACGTR 7 cut(s) 441, 602, 2807, 3593, 4949, 5096, 5594
PpuMI RGGWCCY 1 cut(s) 2995
PscI ACATGT 4 cut(s) 183, 546, 558, 5263
PshBI ATTAAT 1 cut(s) 3216
PsiI TTATAA 1 cut(s) 1004
Psp124BI GAGCTC 1 cut(s) 2442
Psp1406I AACGTT 1 cut(s) 1970
Psp5II RGGWCCY 1 cut(s) 2995
Psp6I CCWGG 9 cut(s) 624, 952, 985, 1546, 3417, 4832, 5286, 5827, 5934
PspCI CACGTG 3 cut(s) 441, 4949, 5096
PspFI CCCAGC 1 cut(s) 2709
PspGI CCWGG 9 cut(s) 624, 952, 985, 1546, 3417, 4832, 5286, 5827, 5934
PspPI GGNCC 7 cut(s) 1993, 2995, 3352, 4190, 4385, 5825, 5938
PspPPI RGGWCCY 1 cut(s) 2995
PsrI GAACNNNNNNTAC 2 cut(s) 5614, 5646
PstI CTGCAG 4 cut(s) 3332, 3651, 4537, 4724
PstNI CAGNNNCTG 2 cut(s) 2074, 3655
PsuI RGATCY 3 cut(s) 628, 1945, 2773
PsyI GACNNNGTC 1 cut(s) 848
PvuII CAGCTG 1 cut(s) 5641
RseI CAYNNNNRTG 6 cut(s) 1221, 1386, 3252, 3940, 4854, 5010
SacI GAGCTC 1 cut(s) 2442
SalI GTCGAC 2 cut(s) 993, 3637
SapI GCTCTTC 2 cut(s) 1266, 3635
Sau96I GGNCC 7 cut(s) 1993, 2995, 3352, 4190, 4385, 5825, 5938
ScaI AGTACT 3 cut(s) 1044, 2961, 4452
SchI GAGTC 8 cut(s) 224, 990, 1000, 1058, 2122, 2486, 2668, 3661
SduI GDGCHC 5 cut(s) 1302, 2387, 2442, 2475, 4527
SexAI ACCWGGT 1 cut(s) 4832
SfcI CTRYAG 9 cut(s) 654, 2756, 3072, 3328, 3647, 4461, 4533, 4720, 5626
SfoI GGCGCC 1 cut(s) 984
SfuI TTCGAA 2 cut(s) 4260, 5526
SinI GGWCC 5 cut(s) 1993, 2995, 3352, 4385, 5825
SmiMI CAYNNNNRTG 6 cut(s) 1221, 1386, 3252, 3940, 4854, 5010
SmlI CTYRAG 8 cut(s) 1660, 2003, 2386, 2492, 3023, 4268, 5039, 5860
SmoI CTYRAG 8 cut(s) 1660, 2003, 2386, 2492, 3023, 4268, 5039, 5860
SnaBI TACGTA 2 cut(s) 3593, 5594
SpeI ACTAGT 2 cut(s) 1855, 4351
SseBI AGGCCT 1 cut(s) 2283
SspDI GGCGCC 1 cut(s) 982
SspI AATATT 4 cut(s) 1964, 4614, 5752, 5778
SstI GAGCTC 1 cut(s) 2442
StuI AGGCCT 1 cut(s) 2283
StyI CCWWGG 7 cut(s) 94, 170, 492, 2020, 2421, 2593, 5676
TatI WGTACW 5 cut(s) 1042, 2959, 4151, 4450, 4816
TauI GCSGC 3 cut(s) 1893, 2307, 5243
TseFI GTSAC 4 cut(s) 487, 2376, 4634, 5956
TseI GCWGC 8 cut(s) 11, 2097, 2307, 2523, 3327, 4532, 4719, 5243
Tsp45I GTSAC 4 cut(s) 487, 2376, 4634, 5956
TspGWI ACGGA 2 cut(s) 1517, 2769
Tth111I GACNNNGTC 1 cut(s) 848
Van91I CCANNNNNTGG 1 cut(s) 4418
Vha464I CTTAAG 2 cut(s) 1660, 3023
VpaK11BI GGWCC 5 cut(s) 1993, 2995, 3352, 4385, 5825
VspI ATTAAT 1 cut(s) 3216
XagI CCTNNNNNAGG 1 cut(s) 4757
XbaI TCTAGA 3 cut(s) 1781, 2107, 2230
XcmI CCANNNNNNNNNTGG 3 cut(s) 45, 3493, 4184
XmaJI CCTAGG 1 cut(s) 2593
XmiI GTMKAC 7 cut(s) 994, 3604, 3638, 3685, 3703, 3823, 4650
XmnI GAANNNNTTC 7 cut(s) 207, 324, 1092, 2919, 3293, 3998, 4784
ZrmI AGTACT 3 cut(s) 1044, 2961, 4452
Zsp2I ATGCAT 3 cut(s) 963, 1494, 5031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.