RLG00000017510

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000307
Physical Location & Seq
Reverse (-)
17684866 .. 17696024
11159 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017510

Sequence Viewer

Length: 5853 bp
ATGGAACTACCTTCATCATCTTCTTCCCCCAACCCCCAATCAGTTCCTTCATCATCTAACTGGGAACATGATGTCTTCCTCAGTTTCAGGGGGGAGGACACCCGCAATACTTTCACAGACCATTTATACTATGCTATGAACCAGAGAGGGATCGACGCATTTAGAGATACTGAAAAGCTTCAGAGGGGGAAATCCATTTCACCAGAACTCCTGAAAGCAATCCAAGAGTCCAAGTTTGCAGTTACAGTTCTTTCAACCAACTATGCTACTTCAACTTGGTGTCTGGATGAACTTGCACATATTCTTGAATGCAGGAAAGCGAGGGGGCTGGAAGTTCTTCCGGTTTTTTATCATGTAGAGCCATCTGAGATAAGGAAGCAAACGGGAAACTATGGCACGGCGTTTGCTAAGCATGAGACAGATTTCAAGGAAAATATGAGAAAGGTGGACAAGTGGAGGAAAGCTTTAGAGGATATAGCCAGTCTCTCCGGATGGCATGTGACAGGGGATAGCCAGAAAGCCTTCAAGAAAGACAATCCACCAGAGGAGTTTGTAGCGCTGTCCAAGAGTTTTCTGCAATATGCTAGTGGCCTTCCTTTAGCTCATGAAGTTCTAGGGTCATACTTGTCTAGACTAAAGGTGGATGCATGGTCAGAGATATTGCAGAGACTTGACGATGATCGGGATAAAGACATTTTTAGTGTGCTTCAAATCAGTTATGATGGATTAGATAAAACGGATAAGAAAATCTTTTTGGATATTGCATGCTTCTTCAATGGCGAGGATCTTGTCCGATGCCTGGGTTGGCATCTTGTTCGGGAAGAATCTTCTTTTCCGGGTGAACGTAGCAGGTTGTGGCTTGACGACCGTGCACACAAGTACGAAGGAAGAAAGTCATGGCGTTTTGAGGATGCTTGTAAAGTGCTCGTGGACAATACGCTCTCTCTGAGAATGCTAATTCACATGGATCTTTCCAACTGCCAATATTTAGCCACGACCCCTGACTTCAGTGAGGTCCCAATGCTTGAGAGATTGATCCTTGAAGGTTGTACAGAGTTATCAGAGGTTCACCCAACAATTGGAGATCTCCAACATCTGGTTTTATTGAATTTGAAAGGTTGTGCAAATCTAGAGAGCCTTCCTCAACCCATCAACTTGAGATCTCTTCAAACTTTTATTCTTTCGGGATGTTCAAAACTCAGAGAGTTTCCAGAGATTGTGGAGAACATGAGTACTTTATCAGAACTTTTTTTAGATGGCACAGCTATAGAGGAGCTGCCTGTATCAATCCAGCATTTGGGAGGCCTTATTTTGCTAAATCTAGTTAGCTGCAAGAAGCTTCTGAGTCTTCCGAGCATCCTTTGTAGAAGTCTGACATCACTGAAATCTCTGTATTTGTCATTCTGCTCAAGTATGGACAGACTGCCGGATGACATCGGCTACTTGGAACACTTGGAGGAGCTTGAAGCGTGTAATACTGCTATAAGAAAAGTGCCCGAGTCCACTTTTCACCTCAAGAATCTTCAATTATTGTGTTTCCATGGCTGTGCTGGACCCACAGGTTTAAAGTTGCCAAGCAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAAGCTCGCAGACGGAGCAATCCCCAGTGACATTGGTGATTTATTCTCACTGCAGAGTCTGGATTTAAGTGAGGACAACTTTGTCACCATACCTGAAAGCATCTCTCAGCTTTCTAAGCTTACAGAAATTTCTCTGTTTAAGTGTAGCAAACTATGGTCATTGCCAAAAGATCCTCCCTCAAGTCTAGAAAATGTAAATGTAGATGGTTGTTCTATGCTGACAATTTCTTCATCTAGTTGGATGAAATATCGGTCTCAGAAGGGTTTGAGTATCATAAACTGCCGAAAACCAGAGGAGGATGACATCTTTCCTGAACTCTATAAGTTTCGTTTGGGAAATCTGAAAGAACTCGATCTTTCCAACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTTAAGAAATTGAACCTTGAAGGTTGTGAAAAGTTATCAGAGGTTCACCCAACCATTGGGTGTCTCCAACATTTGGTGTTTCTGAATTTGAAAGGATGTGTAGATCTAGAGAGCCTTCCCCGTTCCATCAGCTTGAAGTCTCTAAACATATTTGTTCTTTCGGGATGTTCAAAGCTGAAAGAGTTCCCAAACATTAAGGGAGACATGTACAATTTGTCACAACTGCATTTAGATGGGACGGCGTTAAGGCATCTGCAAATACCGATGAAGCCTTTGAATTGCCCTATCTCGATAAACCTGTCAGGCTGCAGGAACCTTTTGACTGTTCCAGTCCTATTATGTCTGAAATTTCTCAATCTGTCGGGCTGCTCACGTATATCCAGATTGCCACTTAACCTGGGAGCCATGGCACATTTGGAGGTGCTTAATGCCTCTGAAACTGCTATAACAGGATTACCCCAGTCCATTTCATACTTGAAGAAACTTAGCGTGTTATCTTTCTGTGGCTGTAAAGGTTTGCAATTGCCTATATGGTTCTCGGATTTAAGCTCTTTGACATCATTAAATCTACGGAGGTGTGGTCTAGCAGAAGAAGTCCTTGATAGCCTCTACGGGTTATCCGCTCTGCAGATTTTGGATTTGAGTGAAAACAATCTTTCAATCATACCTAATGGAATTGGTCGCTTATCCTCTTTGAAGCTATTGAATTTGAGTGAGAACAATTTTGACAATATACCAAATGAAGTTGGTCAGTTGTCCTCTTTGCAGGTTTTAGATTTGAGTGAAAACAACTTTATGAGTATCCCTGAGTGCATCTCTCATCTTTCTGAGCTTACAGAACTACGTTTGTTTAGATGTAGTAAGCTACAGTCCTTGCCAAATAATCTTCCGTTCAATCTAAAACATGTCCATGCACAAGAATGTCATATGTTGAAGAATAACGCAGATACTTTGACAATATGGTCATCTGGAAAGGGGTTCTGTTTCATAAATTGCAAACAATTTAACCAGGATGATGGTCAGCCAAATCATCTCCCAGTTCCAGTTCCTAAAGACCGTATTGAACCACTCCTTCCTATATACATTAAGGACCGAATTTATCGTAAAAGACCATTTGAAATTCGTTTTCCACATGGTACAGGAATTCCCAATTTGTGGAGTTGTTGGAGGAATGGCCCTTCCGTGACAATCCCACTCTTTGATGGTAACAGTACGTGGATAGGACTTGCTCTGTTTGTTGTTTTTGAAATCACTGAGCAAGACATTTTCAACAAAAGGTGGGAATGGGAGGAGACTGTCTGTGAATTTCACACCGATGTTGGGCATGATACTTCCTTGGTTTTTCAAAACTTAATAGACTTCACCACTGGGTCATATGGACTTTGTTGCTATGAACCAAAAGGTGGGAAACTTAGTGGGTTGATTGATAAACCAAGTTCACGACTTCGAGCTTCAGTTTCAACAAAGAGACCAAATTTAGAAGTTAGAGGTTGTGGGATACATCTAATATCACAGGAACATGCTGCAGAATTTGTTAAAAGTTTAGCAACTCAGACCACTCAACATCTTGATTCCAATTTTGATGGACATTGCGAGGACATATTAAATGAGGAAACAACTGGTGCCCTGATGGAACAGGGATCTACCTCAACTTCCAATGAAGATGGCTGCAGCAAAATTAACTCTAAAATCAAACTCAGAGGAGAGCGGTCGATACTCTATGAGGGACGCAATGGGCGTGAGAAGAGTTTCCATTTTTGCTTTCCTGCTTCAGTAATTTCAATCGCGCCCTGGTTCTTCCATCACCATGCAGGGGATGTAACATTGTGTTGCATCTCTAAAAACCTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGCGTATGCCTACTTCGACATCTGGCAATAATAGTTTTTTCTTTTATGTTGATTTGTGCTCTCATGATCATGAAAGTATAGTAATGCACGGTTCCCTTGAGATAAAAAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAGGGTATTAGCGCATTGTTCAGAACCATTAATGCAGAAATGGAGGCCCTAGTGTGTGGAAGCCGTTTAGCTTTCGAGCATGATTTGGAAGACTTGACCTATCAATTAACTGCGGCTGCTGGCACGTTGGGGCAACATGTCCTCACTGAACTATGCGCCCAGGCCCAACCTGTTGATCGGCGCAATGCAGAGGAAGCAATAACGCCTATCAGTTGCTGCTCCTGGTTTAGAAGAAGTGCACTAGTACGACCAAAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCACAATGATAGATGCTATCTCCAGCAACAGCAAGGTTTGCGGGAATCTGACCTTACCCAATTGGTCCCCTTCAGGTCAGTAATATTACTCCACTCCAAAAGCCTTTTGGAATACAGGGATATAAACCAAGAAAACTTTGCTGAAGAAGATAAATTGTTGGGGTTGGCTCGTCATCATGTACGTATAATGGCTGAGACTCTACTTCAGGGAAGCTATAGTTCACCACGGTGGAAGAGATGCCTTCACTTGCTGCTTCGACAGTCCCAGGTGGCTACCCTCAGTCTTGGTGGACACACAATTTCAGTTCTCAAGAATTTTGATCCTTCCTCCCCATACAATGTAATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGACGTCTTCTCAGATGTCTTCTAGGTCCAGGGTGGGAATCAAACTACCTCCAAAATTGCATGAGGTTAAAAATTGGAGAGGACTTGCTATGTGTGTTGCCTTTGAAGTTCATGACCAGTGTCCAACTACCTCCCCGTGCTTGAATCCTATCCCTATGTGTTCCATCAATGAAGAGAAACTCAAGTCGCTGCATCTTGGTAAATTCATTTGGCTAACCTACATACCCCGTATTTTGCTAAGGGAGTTCAGTGTGATTAGAGATGTAGAGGCCAGAATTTATGTTAATTGCAGCGGCTTGACAGTGGAGAAGTGTGGTATACGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAGTGCTGGACCTCTTTCTTCGATAATCTGACTTTCATCCGTCACCTTGTGGAAGCAGATGATCAAAATGTTCAACCATGGACAAGGCATGAACTTCCGATGCTTGAACGGCGTATCAAGGTCTTTAATCCGGATTTAATATATAATGCAATCCCCCCTTCTACTGGAATTCCGAAGTGGTTTAAGGACTGCCACTCAGCTACTGGGCGCTGGAGCTGGGAATTGAAGTTACCACCATCTTCGAGCGACAAAAATTGGAAAGGGCTGGCTCTCTGTGCATCATATCGTATTGTCAGACACTATTTGGAGATGCGTAAGGGCTCAGACTTCAAGTTTAAATTGGAAACTGCAAACAATGGTTTGTCATCTGTCCATCGGTATCAAATGAGCAATGAAGAATTTGAGTCCCTGAAGCGTGATGATAATGTGGATTGCGAGCGTTTTAGTAATGTGGTTGGTGAGCGTTGTCGTAATGGGCATTGTCGTGATTTTGGGGATCATCGTCATATCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGTCCTCATTGCTTCATGCGGTGACACCTGGTGGAACCCGGGCGAGGGCTCTCACCGATTTGTATATGCGGATGACGTTGAAGAGTTTAAGCAGCTCTGTTTCGATCTTCATCGATCCGGTGCCCAACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

1951

Amino Acids

220.98

Weight (kDa)

6.31

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 168 3.5e-50 TIR domain
TIR_2 PF13676 25 - 122 5.3e-15 TIR domain
LRR_14 PF23598 333 - 444 1.1e-06 Leucine-rich repeat region
LRR_13 PF23286 386 - 455 5.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 457 - 634 3.7e-14 Leucine-rich repeat region
LRR_4 PF12799 655 - 692 2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 778 - 893 2.7e-11 Leucine-rich repeat region
LRR_14 PF23598 877 - 972 1.3e-12 Leucine-rich repeat region
LRR_8 PF13855 880 - 920 3.6e-06 Leucine rich repeat
LRR_8 PF13855 909 - 964 8e-08 Leucine rich repeat
C-JID PF20160 1585 - 1709 6.1e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1706, 2995
AatII GACGTC 1 cut(s) 4793
Acc36I ACCTGC 2 cut(s) 840, 2791
AccB1I GGYRCC 2 cut(s) 3656, 5840
AccB7I CCANNNNNTGG 7 cut(s) 1079, 1096, 1297, 2090, 2107, 3189, 3437
AccBSI CCGCTC 2 cut(s) 2657, 3742
AccI GTMKAC 1 cut(s) 5104
AccII CGCG 1 cut(s) 3821
AccIII TCCGGA 2 cut(s) 488, 5284
AciI CCGC 9 cut(s) 103, 2655, 3742, 4229, 4396, 4441, 5079, 5739, 5789
AcuI CTGAAG 8 cut(s) 164, 991, 3471, 3789, 4456, 4563, 4589, 5585
AcvI CACGTG 1 cut(s) 4085
AcyI GRCGYC 1 cut(s) 4790
AdeI CACNNNGTG 2 cut(s) 5203, 5751
AfeI AGCGCT 1 cut(s) 558
AflII CTTAAG 1 cut(s) 2036
AflIII ACRYGT 5 cut(s) 2238, 2938, 4075, 4084, 4252
AhlI ACTAGT 1 cut(s) 4357
AjnI CCWGG 9 cut(s) 798, 2430, 3042, 3824, 4275, 4337, 4665, 4814, 5747
AjuI GAANNNNNNNTTGG 2 cut(s) 737, 769
AleI CACNNNNGTG 1 cut(s) 4627
Alw21I GWGCWC 4 cut(s) 874, 927, 3992, 4357
Alw44I GTGCAC 2 cut(s) 870, 4353
AlwNI CAGNNNCTG 3 cut(s) 1684, 4332, 5357
Ama87I CYCGRG 2 cut(s) 1496, 5758
Aor13HI TCCGGA 2 cut(s) 488, 5284
Aor51HI AGCGCT 1 cut(s) 558
AoxI GGCC 6 cut(s) 589, 1303, 3208, 4161, 4278, 5055
ApaLI GTGCAC 2 cut(s) 870, 4353
AseI ATTAAT 1 cut(s) 4146
Asp700I GAANNNNTTC 5 cut(s) 177, 336, 521, 2216, 3782
AspA2I CCTAGG 1 cut(s) 1615
AspLEI GCGC 6 cut(s) 559, 3823, 4130, 4274, 4299, 5364
AsuC2I CCSGG 3 cut(s) 837, 5759, 5760
AvaI CYCGRG 2 cut(s) 1496, 5758
AvaII GGWCC 7 cut(s) 1015, 1553, 3124, 4050, 4465, 4812, 5163
AvrII CCTAGG 1 cut(s) 1615
BaeGI GKGCMC 5 cut(s) 874, 1497, 3661, 4357, 5845
BanI GGYRCC 2 cut(s) 3656, 5840
BanII GRGCYC 2 cut(s) 5477, 5771
BarI GAAGNNNNNNTAC 2 cut(s) 3928, 3960
BauI CACGAG 1 cut(s) 926
BbrPI CACGTG 1 cut(s) 4085
BbsI GAAGAC 5 cut(s) 67, 1340, 4212, 4785, 4797
Bbv12I GWGCWC 4 cut(s) 874, 927, 3992, 4357
BceAI ACGGC 4 cut(s) 414, 2289, 4164, 5279
BcgI CGANNNNNNTGC 2 cut(s) 797, 831
BciT130I CCWGG 9 cut(s) 800, 2432, 3044, 3826, 4277, 4339, 4667, 4816, 5749
BciVI GTATCC 2 cut(s) 2846, 3525
BclI TGATCA 3 cut(s) 3889, 3997, 5215
BcnI CCSGG 3 cut(s) 837, 5759, 5760
BcuI ACTAGT 1 cut(s) 4357
BfmI CTRYAG 8 cut(s) 1266, 1676, 2341, 2660, 2900, 3558, 3703, 4615
BfoI RGCGCY 2 cut(s) 560, 5365
BfrI CTTAAG 1 cut(s) 2036
BfuAI ACCTGC 2 cut(s) 840, 2791
BfuI GTATCC 2 cut(s) 2846, 3525
BglII AGATCT 4 cut(s) 1084, 1160, 1595, 2137
BlnI CCTAGG 1 cut(s) 1615
BlpI GCTNAGC 1 cut(s) 408
BmcAI AGTACT 1 cut(s) 1234
Bme18I GGWCC 7 cut(s) 1015, 1553, 3124, 4050, 4465, 4812, 5163
BmeT110I CYCGRG 2 cut(s) 1496, 5758
BmrI ACTGGG 6 cut(s) 70, 1644, 2488, 3065, 3411, 5367
BmuI ACTGGG 6 cut(s) 70, 1644, 2488, 3065, 3411, 5367
BoxI GACNNNNGTC 1 cut(s) 4905
BpiI GAAGAC 5 cut(s) 67, 1340, 4212, 4785, 4797
BplI GAGNNNNNCTC 4 cut(s) 1126, 1158, 2834, 2866
BpmI CTGGAG 2 cut(s) 4406, 5386
Bpu10I CCTNAGC 1 cut(s) 5024
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 8 cut(s) 1046, 1177, 1393, 1499, 1789, 4049, 4694, 4952
BpuMI CCSGG 3 cut(s) 837, 5759, 5760
Bsa29I ATCGAT 1 cut(s) 5833
BsaAI YACGTR 4 cut(s) 2408, 3249, 4085, 4583
BsaBI GATNNNNATC 1 cut(s) 5829
BsaHI GRCGYC 1 cut(s) 4790
BsaI GGTCTC 2 cut(s) 1884, 3496
BsaWI WCCGGW 4 cut(s) 340, 488, 5284, 5837
BsaXI ACNNNNNCTCC 4 cut(s) 192, 222, 2599, 2629
Bse118I RCCGGY 1 cut(s) 5118
Bse3DI GCAATG 6 cut(s) 1784, 3622, 3772, 4306, 5551, 5727
Bse8I GATNNNNATC 1 cut(s) 5829
BseAI TCCGGA 2 cut(s) 488, 5284
BseBI CCWGG 9 cut(s) 800, 2432, 3044, 3826, 4277, 4339, 4667, 4816, 5749
BseCI ATCGAT 1 cut(s) 5833
BseJI GATNNNNATC 1 cut(s) 5829
BseMI GCAATG 6 cut(s) 1784, 3622, 3772, 4306, 5551, 5727
BseRI GAGGAG 6 cut(s) 560, 1286, 1472, 1934, 3338, 3750
BseSI GKGCMC 5 cut(s) 874, 1497, 3661, 4357, 5845
BseYI CCCAGC 1 cut(s) 5370
BsgI GTGCAG 1 cut(s) 4385
Bsh1236I CGCG 1 cut(s) 3821
Bsh1285I CGRYCG 2 cut(s) 868, 3746
BshFI GGCC 6 cut(s) 591, 1305, 3210, 4163, 4280, 5057
BshNI GGYRCC 2 cut(s) 3656, 5840
BshVI ATCGAT 1 cut(s) 5833
BsiEI CGRYCG 2 cut(s) 868, 3746
BsiHKAI GWGCWC 4 cut(s) 874, 927, 3992, 4357
BsiHKCI CYCGRG 2 cut(s) 1496, 5758
BsiSI CCGG 8 cut(s) 341, 489, 836, 1427, 5119, 5285, 5759, 5838
BslFI GGGAC 7 cut(s) 1001, 2284, 3774, 4063, 4451, 4648, 5545
BsmBI CGTCTC 2 cut(s) 4781, 5114
BsmFI GGGAC 7 cut(s) 1001, 2284, 3774, 4063, 4451, 4648, 5545
BsmI GAATGC 2 cut(s) 314, 957
BsnI GGCC 6 cut(s) 591, 1305, 3210, 4163, 4280, 5057
Bso31I GGTCTC 2 cut(s) 1884, 3496
BsoBI CYCGRG 2 cut(s) 1496, 5758
Bsp1286I GDGCHC 9 cut(s) 874, 927, 1497, 3661, 3992, 4357, 5477, 5771, 5845
Bsp13I TCCGGA 2 cut(s) 488, 5284
Bsp1407I TGTACA 2 cut(s) 1049, 2241
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 3 cut(s) 1540, 2439, 5231
BspACI CCGC 9 cut(s) 103, 2655, 3742, 4229, 4396, 4441, 5079, 5739, 5789
BspANI GGCC 6 cut(s) 591, 1305, 3210, 4163, 4280, 5057
BspDI ATCGAT 1 cut(s) 5833
BspEI TCCGGA 2 cut(s) 488, 5284
BspFNI CGCG 1 cut(s) 3821
BspHI TCATGA 4 cut(s) 604, 3994, 4000, 4897
BspMAI CTGCAG 5 cut(s) 1680, 2345, 2664, 3562, 3707
BspMI ACCTGC 2 cut(s) 840, 2791
BspT107I GGYRCC 2 cut(s) 3656, 5840
BspTI CTTAAG 1 cut(s) 2036
BspTNI GGTCTC 2 cut(s) 1884, 3496
BsrBI CCGCTC 2 cut(s) 2657, 3742
BsrDI GCAATG 6 cut(s) 1784, 3622, 3772, 4306, 5551, 5727
BsrFI RCCGGY 1 cut(s) 5118
BsrGI TGTACA 2 cut(s) 1049, 2241
BssAI RCCGGY 1 cut(s) 5118
BssNAI GTATAC 1 cut(s) 5105
BssNI GRCGYC 1 cut(s) 4790
BssSI CACGAG 1 cut(s) 926
BssT1I CCWWGG 5 cut(s) 1540, 1615, 2439, 3369, 5231
Bst1107I GTATAC 1 cut(s) 5105
Bst2BI CACGAG 1 cut(s) 926
Bst2UI CCWGG 9 cut(s) 800, 2432, 3044, 3826, 4277, 4339, 4667, 4816, 5749
Bst6I CTCTTC 6 cut(s) 1170, 3773, 4628, 4953, 5121, 5796
BstACI GRCGYC 1 cut(s) 4790
BstAFI CTTAAG 1 cut(s) 2036
BstAPI GCANNNNNTGC 1 cut(s) 4438
BstAUI TGTACA 2 cut(s) 1049, 2241
BstBAI YACGTR 4 cut(s) 2408, 3249, 4085, 4583
BstC8I GCNNGC 5 cut(s) 766, 1632, 4237, 5421, 5591
BstDSI CCRYGG 4 cut(s) 1540, 2439, 4625, 5231
BstFNI CGCG 1 cut(s) 3821
BstH2I RGCGCY 2 cut(s) 560, 5365
BstHHI GCGC 6 cut(s) 559, 3823, 4130, 4274, 4299, 5364
BstMCI CGRYCG 2 cut(s) 868, 3746
BstMWI GCNNNNNNNGC 6 cut(s) 1640, 1741, 4310, 4438, 5263, 5429
BstNI CCWGG 9 cut(s) 800, 2432, 3044, 3826, 4277, 4339, 4667, 4816, 5749
BstNSI RCATGY 7 cut(s) 500, 768, 2242, 2942, 3557, 4079, 4256
BstPAI GACNNNNGTC 1 cut(s) 4905
BstSFI CTRYAG 8 cut(s) 1266, 1676, 2341, 2660, 2900, 3558, 3703, 4615
BstSLI GKGCMC 5 cut(s) 874, 1497, 3661, 4357, 5845
BstSNI TACGTA 1 cut(s) 4583
BstUI CGCG 1 cut(s) 3821
BstV2I GAAGAC 5 cut(s) 67, 1340, 4212, 4785, 4797
BstX2I RGATCY 9 cut(s) 784, 967, 1084, 1160, 1595, 1795, 2007, 2137, 3674
BstXI CCANNNNNNTGG 2 cut(s) 3050, 5691
BstYI RGATCY 9 cut(s) 784, 967, 1084, 1160, 1595, 1795, 2007, 2137, 3674
BstZ17I GTATAC 1 cut(s) 5105
Bsu15I ATCGAT 1 cut(s) 5833
BsuI GTATCC 2 cut(s) 2846, 3525
BsuRI GGCC 6 cut(s) 591, 1305, 3210, 4163, 4280, 5057
BsuTUI ATCGAT 1 cut(s) 5833
BtgI CCRYGG 4 cut(s) 1540, 2439, 4625, 5231
BtsI GCAGTG 1 cut(s) 1673
BveI ACCTGC 2 cut(s) 840, 2791
Cac8I GCNNGC 5 cut(s) 766, 1632, 4237, 5421, 5591
CaiI CAGNNNCTG 3 cut(s) 1684, 4332, 5357
CciI TCATGA 4 cut(s) 604, 3994, 4000, 4897
CfoI GCGC 6 cut(s) 559, 3823, 4130, 4274, 4299, 5364
Cfr10I RCCGGY 1 cut(s) 5118
Cfr9I CCCGGG 1 cut(s) 5758
ClaI ATCGAT 1 cut(s) 5833
CseI GACGC 3 cut(s) 164, 3771, 5697
CsiI ACCWGGT 1 cut(s) 5747
DraI TTTAAA 2 cut(s) 1566, 5491
DraIII CACNNNGTG 2 cut(s) 5203, 5751
DrdI GACNNNNNNGTC 2 cut(s) 1706, 2995
DseDI GACNNNNNNGTC 2 cut(s) 1706, 2995
Eam1104I CTCTTC 6 cut(s) 1170, 3773, 4628, 4953, 5121, 5796
EarI CTCTTC 6 cut(s) 1170, 3773, 4628, 4953, 5121, 5796
Eco105I TACGTA 1 cut(s) 4583
Eco130I CCWWGG 5 cut(s) 1540, 1615, 2439, 3369, 5231
Eco147I AGGCCT 1 cut(s) 1305
Eco24I GRGCYC 2 cut(s) 5477, 5771
Eco31I GGTCTC 2 cut(s) 1884, 3496
Eco47I GGWCC 7 cut(s) 1015, 1553, 3124, 4050, 4465, 4812, 5163
Eco47III AGCGCT 1 cut(s) 558
Eco57I CTGAAG 8 cut(s) 164, 991, 3471, 3789, 4456, 4563, 4589, 5585
Eco72I CACGTG 1 cut(s) 4085
Eco88I CYCGRG 2 cut(s) 1496, 5758
EcoO109I RGGNCCY 3 cut(s) 1015, 4050, 4162
EcoRI GAATTC 2 cut(s) 3177, 5322
EcoRII CCWGG 9 cut(s) 798, 2430, 3042, 3824, 4275, 4337, 4665, 4814, 5747
EcoT14I CCWWGG 5 cut(s) 1540, 1615, 2439, 3369, 5231
EcoT22I ATGCAT 1 cut(s) 649
EcoT38I GRGCYC 2 cut(s) 5477, 5771
ErhI CCWWGG 5 cut(s) 1540, 1615, 2439, 3369, 5231
Esp3I CGTCTC 2 cut(s) 4781, 5114
FalI AAGNNNNNCTT 4 cut(s) 734, 766, 2616, 2648
FaqI GGGAC 7 cut(s) 1001, 2284, 3774, 4063, 4451, 4648, 5545
FauI CCCGC 2 cut(s) 110, 4434
FauNDI CATATG 2 cut(s) 2961, 3409
FbaI TGATCA 3 cut(s) 3889, 3997, 5215
FblI GTMKAC 1 cut(s) 5104
FriOI GRGCYC 2 cut(s) 5477, 5771
GlaI GCGC 6 cut(s) 558, 3822, 4129, 4273, 4298, 5363
GsaI CCCAGC 1 cut(s) 5374
GsuI CTGGAG 2 cut(s) 4406, 5386
HaeII RGCGCY 2 cut(s) 560, 5365
HaeIII GGCC 6 cut(s) 591, 1305, 3210, 4163, 4280, 5057
HapII CCGG 8 cut(s) 341, 489, 836, 1427, 5119, 5285, 5759, 5838
HgaI GACGC 3 cut(s) 164, 3771, 5697
HhaI GCGC 6 cut(s) 559, 3823, 4130, 4274, 4299, 5364
Hin1I GRCGYC 1 cut(s) 4790
Hin6I GCGC 6 cut(s) 557, 3821, 4128, 4272, 4297, 5362
HinP1I GCGC 6 cut(s) 557, 3821, 4128, 4272, 4297, 5362
HindIII AAGCTT 4 cut(s) 176, 462, 1337, 1742
HpaII CCGG 8 cut(s) 341, 489, 836, 1427, 5119, 5285, 5759, 5838
Hpy99I CGWCG 2 cut(s) 158, 5691
HpyF10VI GCNNNNNNNGC 6 cut(s) 1640, 1741, 4310, 4438, 5263, 5429
Hsp92I GRCGYC 1 cut(s) 4790
HspAI GCGC 6 cut(s) 557, 3821, 4128, 4272, 4297, 5362
Kpn2I TCCGGA 2 cut(s) 488, 5284
Ksp22I TGATCA 3 cut(s) 3889, 3997, 5215
LmnI GCTCC 7 cut(s) 1273, 1459, 1640, 2435, 4340, 4381, 5367
MabI ACCWGGT 1 cut(s) 5747
MbiI CCGCTC 2 cut(s) 2657, 3742
MfeI CAATTG 4 cut(s) 1077, 2555, 4389, 4460
MflI RGATCY 9 cut(s) 784, 967, 1084, 1160, 1595, 1795, 2007, 2137, 3674
MhlI GDGCHC 9 cut(s) 874, 927, 1497, 3661, 3992, 4357, 5477, 5771, 5845
MlyI GAGTC 7 cut(s) 236, 1354, 1508, 1690, 4591, 5567, 5726
MmeI TCCRAC 7 cut(s) 999, 1114, 1844, 2010, 2125, 3179, 4934
Mph1103I ATGCAT 1 cut(s) 649
MroI TCCGGA 2 cut(s) 488, 5284
MroXI GAANNNNTTC 5 cut(s) 177, 336, 521, 2216, 3782
MslI CAYNNNNRTG 9 cut(s) 1545, 2265, 2943, 2953, 3348, 3840, 3999, 4627, 5637
MspA1I CMGCKG 1 cut(s) 5079
MspCI CTTAAG 1 cut(s) 2036
MspI CCGG 8 cut(s) 341, 489, 836, 1427, 5119, 5285, 5759, 5838
MunI CAATTG 4 cut(s) 1077, 2555, 4389, 4460
Mva1269I GAATGC 2 cut(s) 314, 957
MvaI CCWGG 9 cut(s) 800, 2432, 3044, 3826, 4277, 4339, 4667, 4816, 5749
MvnI CGCG 1 cut(s) 3821
MwoI GCNNNNNNNGC 6 cut(s) 1640, 1741, 4310, 4438, 5263, 5429
NciI CCSGG 3 cut(s) 837, 5759, 5760
NcoI CCATGG 3 cut(s) 1540, 2439, 5231
NdeI CATATG 2 cut(s) 2961, 3409
NmeAIII GCCGAG 1 cut(s) 3910
NmuCI GTSAC 7 cut(s) 499, 1652, 1708, 2250, 3217, 5195, 5741
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 7 cut(s) 500, 768, 2242, 2942, 3557, 4079, 4256
OliI CACNNNNGTG 1 cut(s) 4627
PaeI GCATGC 1 cut(s) 768
PagI TCATGA 4 cut(s) 604, 3994, 4000, 4897
PceI AGGCCT 1 cut(s) 1305
PciI ACATGT 4 cut(s) 2238, 2938, 4075, 4252
PcsI WCGNNNNNNNCGW 1 cut(s) 3769
PctI GAATGC 2 cut(s) 314, 957
PdmI GAANNNNTTC 5 cut(s) 177, 336, 521, 2216, 3782
PfeI GAWTC 7 cut(s) 824, 1519, 3605, 4108, 4445, 4824, 4930
PflMI CCANNNNNTGG 7 cut(s) 1079, 1096, 1297, 2090, 2107, 3189, 3437
PleI GAGTC 7 cut(s) 235, 1353, 1507, 1689, 4591, 5566, 5725
PmaCI CACGTG 1 cut(s) 4085
PmlI CACGTG 1 cut(s) 4085
PpsI GAGTC 7 cut(s) 235, 1353, 1507, 1689, 4591, 5566, 5725
Ppu21I YACGTR 4 cut(s) 2408, 3249, 4085, 4583
PpuMI RGGWCCY 2 cut(s) 1015, 4050
PscI ACATGT 4 cut(s) 2238, 2938, 4075, 4252
PshAI GACNNNNGTC 1 cut(s) 4905
PshBI ATTAAT 1 cut(s) 4146
Psp5II RGGWCCY 2 cut(s) 1015, 4050
Psp6I CCWGG 9 cut(s) 798, 2430, 3042, 3824, 4275, 4337, 4665, 4814, 5747
PspCI CACGTG 1 cut(s) 4085
PspFI CCCAGC 1 cut(s) 5370
PspGI CCWGG 9 cut(s) 798, 2430, 3042, 3824, 4275, 4337, 4665, 4814, 5747
PspPPI RGGWCCY 2 cut(s) 1015, 4050
PsrI GAACNNNNNNTAC 2 cut(s) 4071, 4103
PstI CTGCAG 5 cut(s) 1680, 2345, 2664, 3562, 3707
PstNI CAGNNNCTG 3 cut(s) 1684, 4332, 5357
PsuI RGATCY 9 cut(s) 784, 967, 1084, 1160, 1595, 1795, 2007, 2137, 3674
RseI CAYNNNNRTG 9 cut(s) 1545, 2265, 2943, 2953, 3348, 3840, 3999, 4627, 5637
ScaI AGTACT 1 cut(s) 1234
SchI GAGTC 7 cut(s) 236, 1354, 1508, 1690, 4591, 5567, 5726
SduI GDGCHC 9 cut(s) 874, 927, 1497, 3661, 3992, 4357, 5477, 5771, 5845
SexAI ACCWGGT 1 cut(s) 5747
SfcI CTRYAG 8 cut(s) 1266, 1676, 2341, 2660, 2900, 3558, 3703, 4615
SinI GGWCC 7 cut(s) 1015, 1553, 3124, 4050, 4465, 4812, 5163
SmaI CCCGGG 1 cut(s) 5760
SmiMI CAYNNNNRTG 9 cut(s) 1545, 2265, 2943, 2953, 3348, 3840, 3999, 4627, 5637
SmlI CTYRAG 9 cut(s) 1025, 1156, 1408, 1514, 1804, 2036, 4028, 4709, 4967
SmoI CTYRAG 9 cut(s) 1025, 1156, 1408, 1514, 1804, 2036, 4028, 4709, 4967
SnaBI TACGTA 1 cut(s) 4583
SpeI ACTAGT 1 cut(s) 4357
SphI GCATGC 1 cut(s) 768
SseBI AGGCCT 1 cut(s) 1305
SsiI CCGC 9 cut(s) 103, 2655, 3742, 4229, 4396, 4441, 5079, 5739, 5789
SspI AATATT 2 cut(s) 986, 4485
StuI AGGCCT 1 cut(s) 1305
StyI CCWWGG 5 cut(s) 1540, 1615, 2439, 3369, 5231
TaqII GACCGA 2 cut(s) 1866, 3141
TatI WGTACW 3 cut(s) 1049, 1232, 2241
TauI GCSGC 3 cut(s) 4232, 4399, 5082
TfiI GAWTC 7 cut(s) 824, 1519, 3605, 4108, 4445, 4824, 4930
TseFI GTSAC 7 cut(s) 499, 1652, 1708, 2250, 3217, 5195, 5741
Tsp45I GTSAC 7 cut(s) 499, 1652, 1708, 2250, 3217, 5195, 5741
TspGWI ACGGA 6 cut(s) 752, 1653, 2620, 2913, 3205, 5183
TspMI CCCGGG 1 cut(s) 5758
Van91I CCANNNNNTGG 7 cut(s) 1079, 1096, 1297, 2090, 2107, 3189, 3437
Vha464I CTTAAG 1 cut(s) 2036
VneI GTGCAC 2 cut(s) 870, 4353
VpaK11BI GGWCC 7 cut(s) 1015, 1553, 3124, 4050, 4465, 4812, 5163
VspI ATTAAT 1 cut(s) 4146
XbaI TCTAGA 4 cut(s) 629, 1129, 1810, 2140
XceI RCATGY 7 cut(s) 500, 768, 2242, 2942, 3557, 4079, 4256
XcmI CCANNNNNNNNNTGG 4 cut(s) 1547, 2446, 4504, 5354
XmaI CCCGGG 1 cut(s) 5758
XmaJI CCTAGG 1 cut(s) 1615
XmiI GTMKAC 1 cut(s) 5104
XmnI GAANNNNTTC 5 cut(s) 177, 336, 521, 2216, 3782
ZraI GACGTC 1 cut(s) 4791
ZrmI AGTACT 1 cut(s) 1234
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.