RLG00000034092

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000307
Physical Location & Seq
Reverse (-)
36664070 .. 36674258
10189 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034092

Sequence Viewer

Length: 6261 bp
ATGAGCCACAAGAGAGAGTTTGATCCTGATGATCCATATACCCAAATTGCTTCTACATCATCAGCAATGGATTACAGCAACACCGAAATAGGATCTTCATCATCAGCAATGGATTACAGCAACACCGAAATAGGATCTTCATCATCATCCGACTCTTCAGCATCAGACTTGAAATCATTCGATGTCTTCCTCAGTTTCAGGGGCGAGGACACCCGCCACAGTTTTACCGATCATTTGTACTATGATTTGAATAAGAAGGGAATTCATACCTTTCGGGATGCTGAGAAACTAAGAAAAGGGGAAGCCATTGGACCAGCACTTTTGGAAGCTATTAAAGAATCAAGGTTTGCAGTGGTCATTCTTACACCAGAATATGCTTCCTCAGCGTGGTGTTTGGATGAACTCGCCCACATTATTAAATGCAAGGAAGAGAGTGGACTGAAAGTTTATCCCGTCTTTTGTCATGTCCAACCATCAGAGGTACGATGGCAGAAAACAGGAAAGTTTCGTGAGGCCTTTGATAAACATGAGGAACGATACGGAGGCACTACGGACAGAGTGGAAAACTGGAGAAAAGCTTTGAGTGAAGTAGCCAATCTCTCCGGATGGGATTTGCAAAACAAGTATGAGACAGAAGTTATTCAAGAAATAGCTAGAAAGATTTCAAAAATCTTGCCCTTTATGGTCTCTAGTGCTAGCAAAAATATAGTTGGAATTGATGTTCGTATAGAGACATTGAAGAATTACTTGGCTTTGTGGTCTACATATGACATTCGCACCATAGGGATTTGGGGAATGGGGGGCATAGGTAAAACAACTCTTGCAGAAGAATTTTTCAAAAGATTTCGTAACCAATTTGATGCTAGCGAGTTTGTTGCCGATGTTAGACTGAAAGCGCGGAGGAATGGTCTAGTTTACTTGCAAAAACTTCTTTCTGCATCCTTATTGGAGAGTGATGATAATATACCGTCCGTTCGGATGGGGAGTGAATTCTTAAGGAAAAGGCTAAAGCAAAAGGTGCTTATCATTTTGGACGATGTTGATGAATTAAAACAAATAGAAGCTTTAGCAACAGGAAGGCCGGGTGAACTTAATTGGTTGGGTCAAGGAAGTCGAGTGATTATAACAACTAGAGATAAGCAGCCATTGTTGACATGGGGAGTACAAGAAGAGAACATATACGAGGTTGAGAAACTGACTGATGATGAGGCTCTTCAGCTTTTATGTCAAAAAGCATTCAACAGTGAACGTGCTCCAGATGATTATAAAGAGCTGTCCAACAAGTTGTTAAATTATGTTAGTGGCCTTCCTCTAGCTATTGAAGTTCTGGGTTCATACTTGTCTGGGAGAGAGGTAAGTGAATGGTTGGAGGCATTGGCTAGACTTCATGAAGATCCTGAAAAAGGCATTTTCAGTGTTCTTCAAATAAGTTTTGAAGGATTAAGAACAACTGAGAAGAATATCTTTTTGGATATTGCATGTTTCTTCAGAGGTGAGGATCAAGCACGTGTAAGGAAGATATTAAATAGCTGTCGCTTTTTCCCTGACAACGGTATAAAAAACCTTGTTTATAAATCTCTGCTCAAAATTGAAAACAATAAACTGTGGATGCATGATTTATTGCAACAAATGGGCTGGCATATTGTTCGTCAAGAATCTCGTCTTCATCCAGGCGAACGTAGTAGGTTGTGGATTAATGATAATACCTACTTGTACGAACGCGATGAATCATGGCTTGACACGGATGCCTGTGATGTACTCACAGAAAATACGGGAACGAAAGATGTTGAAGGCATATTCCTACGCTTGCCTGAAAAATTGGAAATCTGCTTAAATGCTGACCCGTTCTCTAATATGAGGACTCTCGACTTCAGTACGGTCCCAAATCTTGAGCGGCTAAACTTTGAAGGTTGTAAAAGATTATCAGAAGTTCACCCAACAATTGGGGATCTAAAACATCTAGTCTTATTGAATATGAAAAATTGTGAAAGTCTAGAAAGCCTTGTCAACTCCATCCGCTTGACATCTCTTGAAACGTTTATTCTTTCCGGATGTTCAAAGCTCAAAGAGTTTCCAGAGATTGTCGGAAGAATTAAATCTTTGGCAGAACTTTACTTAGATGGGACGGCTATAAGGGAGCTGCCGATATCAATCCAACAATTGAGTGGCCTTATTTTGCTGAATTTAAAAGGCTGCAAATACCTTCTGGGTCTTCCAAGCGTCGTTTGTAATCTGATGTCTCTGAAGTATCTATATCTCTCAGGCTGCTCTCGTATTGACCAACTGCCAGAACATCTAGGGAGTTTGGAACATTTGCAGGTGCTTGATGCCTGTAAAACTGCTATAAGAAAAGTTCCATCATCCATCTTACTTCTGAAGAACCCCAAACAGTTGTGTTTTCCAGGAAGTAATGATCTGCAGATGGAATTCTCTGAATCCAGTGGTTTCCGCTACTCATCCTCACATTCATGTTCATTGTTGGCATTAAATACCCCCTATGATCTTTTCTTCTGTTTCTCACAACCTGTTTCAGATTTGAATGAAAACAAAATGTTGATCAGCAAACCTGAAATCATCTCCGATGGATATTTGCAGGAGCTTGATACCTGGAGCACTCTGAATTGGAGTTGCAGTTTCCGTGAAACAGATTTGAGAAATAATTTTCATTTGATGCTTTGTGACAATTCTCCTCAGATGTTCTTTGATTCCTGTCAATATTCATCTTTGACATCTTTACTTCTGAGAGGATGCAAGCTAGGCGAAAGAGATCGAATTCATCATGACAAGCCATTTGAAATTCGTTTTCGTCATGATGAACTTTTCAGTCGTTTTGGAGACCTTAGTACAAATGAAATTGGTGTTCCTGATGATGATGGAATTCCGCACTGGTGCACTCAGTGGAACACTGGTTCTTCTGTTACATTGCAACTAACTGGTCCAGAAGACAAGACTAATAGTACGTGGATGGGATTAGCCTTATATGTTGCTTTTGAAGACTTTCGGAATTATCGAGAGAAAGAAATTCTCTGTCATCTGAGCATCGACGAAGGTGGTCCTCAAATTCCTTTAGTTTTGCAAGACTTGGATGATGAGTCACTTGGACTCTGTTGCTACTTGCCACGAGGCTGCGGGGGGTTTGCAGGACAGCTGGATAAAGCCAGTCGAATTAATGCTACATTTTTACCAACTACTTTACCAGTGAAATCGTGTGGGATACAACCAATTTCCCAGCATGATGCTCCAATGTTTGCTCAGGACTTAGCCCGGTCTGCTTTTGCTGAGGCACGTGCTCAACAGGAAAAATTGTTCAAGTACTTTAATGGGGAGGCACGTGCTAATTGCAAGGCAGGTGCTTATGAAGTTATTCGACATGGTAAGAGAATGTTGGATAAGGCAACGAAATTGGAAAGATCTGGAGACGTGCTGGTACCTGCGTACAATTCTTTAAATGTGAATCGACACAAGTGGAAAATGGAGGAAGAGGCAAAGCTGTTCAGATTGCTTTCAGGGCTCTACCAGGGAAGCAATGGACGTCAGAAGAAATTTCATTTTTGTTTTCCAGGTCCAGTAATTTCGCCCTGGTTCATCAATCACTACGCAGGGAATATAGCACTTTGTGGCCTTCCTCAGAACTTAATCGATGATAAGAGATGGATCGGATTAGAAGTTTATGTTGTGTTTCGTTGGTCACCAACAGTTGGTTACAGTGACCACAATCTTTCTGATTCCTTCCTTCATGTTGATTTAATGGCACATGAAGGTAGTGTAATGCATTCGTCCATCAAAATTGAGAGTTGTTCTGGGTCAGATCAACTTATTGTACTACATGTACCACGGATGCATTTTCCAGAACAGTTGACTCATTATCAGGTTATTAGCACGTTGTTTACAACCAGTACCCCAATTGTTGAGATTATAGTGTGCGGGAGCCGTTTGGTATATGAGCATGATCTGGAAGATTTAATCCTTTCATTAACTGCGTGTACATTGAGGAAGCCAGATTCAATTGGCCAAGTTTATTCCCAGGCCCAAATATATGTTGAAACTATGGAAAAGAAAGCAGATGAAAATATCCAGTGCAATGCAAAGGGAGGAGAAATGTCATCCACTGATGGCCATTCATCGTTTCAAAGAAGTGTCAAAAAAATTGAAGTACCAGATAAAGTTCGCAGTTCAATATTGCAGAAATATAGATGCCATTTCCAAGGAAAGCAAGGTATACCAGAGTCTGGCAAAATAATTGTCCACTCTAAATCCCTTCATCTGGACAATAGCATCAAAGAAAATTTTACTGGAAAAGAATCAATAATGTTGTCGTTGGCATGTCATCATTTGCATATAGTGGCTGAGACTCACTTCTGGGGAAGCTATAGTGCCCGACAGTGGAAGACATGCCTAGAGTTGTTGCTGCAATATTCTGAGGTGGCAACTCTCAGTCTTAATGGACATGTAATCTCTGTAATGAAGCATTTTAGTCCCTCCTCGCCATTCAATTTTTGTTTCCCTAAAGATGAAGTGCTGCTCAAGTGGATGAGAGATCGTCGATTCCGATTGCCTCAACAACTCTATGATGATAAAAGTTTCAGGGGATTTGTTATATGTGCAACTTTTTCATTTGACGATGATTCTGCCATGAACAATCTGGCTTCAGAAATTACTGTTGAACTCTTGTGTCACTTGAGTTACAATGGTTCCAGGTTTTGCGTGAACGCTGCCCCCATGCTTACGATCACCAAGGAGAAATTCCAGTGGCTTTGTTTCCGTGATTTCATTTGGGTTACGTATGTACCCCTTGTTATGCTAGCGGAGGTCAAAGTAATCAGTGATATAGAGGCTGATGTATGTAGTAATTGCCCAGGCTTGAGGGTGGACTGCGCTTATACACGTGTCTTGTACCGGCAAGAGGAGGAGGAATTTAAGCAAGCAATAACTCAGTGCTGGACCTCCTTCTTTGATAATATCGATCTCCTCCGTCGGTTTGTACAAGCAGATGCTGAAAAAGGCGTAGAGACGAAGCACGAACTTCCAATGCTCGGCGGAAGTATGAAGGACCTTGATAGCAACTTGATGTATGACGCAACACACCCATCTACTGAAATTCCGGATTGGTTTGGACATCCAAATGAAAGGTCTGTAGAGATCCAGTTACCACCATCTCTTGGCAGGGACGAAAATTGGATAGGAGTGGCTATATGTGCATCATATTTTCCCGTACAATTGAATCCTCAAGTTTCTGCGTACTTAAAATGTGTTTTGCGAACTGAGATGAATAACTTGAAATCTGTCCACCGCTATCAGATGACCAATGAAGAAATCAAGTGCGTAGAATCTTCTGAGAGAAAATTCATTTGGCTATCCTATCTTCCACGACACTGGTTTTTCTACCAGTTAAATGATGAGTCTGCTCTCAGGGTTTCTTCGTTTTCAAGTTCACCTGGTTTGTGGTTTAAGGAGTGCGGTCTGCGTCTTGTGTACCGGCAAGATGAGGAAGAGTTTAAGCGACTCTGTCGATCGGCGTCGTACGACGCCTCTTCGTCTCTTGACGTACTTCTCGAGATAGGAGAACGCGACGTTGAAATTGAACGCCGCTTTGCGCTCGGAGAACCAGCGTGGGGCCTCGCAAATGGTGATACTTCAAAGACAACTGGCCAAGAAATTCTGGTCCAGCCCATTCATCACAGTCTACCAGTTCATCAGGCTCAGAACCCTTACCCTCAGACTCTGCAGAAACATGGAATTCTTCCACTAGTCAACAGGAAAATAGCCGATTTTGAATTAGGGCCGCTACCTCTAAAGATTATGCCGCGTTCTCGGGGGTTTAGGCCTCAAGGAAGGAATGTTGCTAGTGCTGTAAAGGTTGTAGAGTCAGCATTGGCTTTGCAAGAAGCTGGGTGCTTTTCTGTTGTTTTGGAATGTGCGCCACCACTAATTCCTACAATTGGCATTGGAGCAGTGCCTTTTTGCAGTGGACAGTTTACCAATGATCTTCTTGGAATGATGCAGCACCCACATCATGCCAAGGTTACCCCTAAATTGTGTAAGCAGTTTGCACAGGTTGGAGATGTCATCAACAAAGCTCTTGTAGACTACAAGGAAGAATTGACTAGTGGAACATTCCCAGGTGCTGCCCGCAGCCCATACAAGATTAACCCTGCTGATGTTGATGGCTTTTTAAGCGAGTTACAAAAAATGGGTTTGGACAAGGCTGCATCTGTGGTGGCTGAAGCAGCTGAGAAGATAAATACAAACAAATCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

2087

Amino Acids

236.59

Weight (kDa)

5.93

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 60 - 234 3.4e-50 TIR domain
TIR_2 PF13676 62 - 158 2.3e-14 TIR domain
NB-ARC PF00931 254 - 416 3.6e-18 NB-ARC domain
NACHT PF05729 259 - 410 3.2e-09 NACHT domain
WHD_ROQ1 PF23282 484 - 553 1.7e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 674 - 759 1.6e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 727 - 800 1.1e-10 Leucine-rich repeat region
C-JID PF20160 1696 - 1830 6.6e-09 C-JID domain
Pantoate_transf PF02548 1937 - 2041 1.2e-19 Ketopantoate hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1124, 1266, 1572
AarI CACCTGC 2 cut(s) 2308, 3338
AatII GACGTC 1 cut(s) 3535
Acc36I ACCTGC 3 cut(s) 2308, 3338, 3439
Acc65I GGTACC 1 cut(s) 3427
AccB1I GGYRCC 1 cut(s) 3427
AccB7I CCANNNNNTGG 4 cut(s) 1943, 3698, 4229, 5159
AccBSI CCGCTC 1 cut(s) 1894
AccI GTMKAC 4 cut(s) 761, 4219, 5683, 6084
AccII CGCG 4 cut(s) 898, 1722, 5568, 5806
AccIII TCCGGA 3 cut(s) 602, 2048, 5101
AclI AACGTT 1 cut(s) 2036
AclWI GGATC 9 cut(s) 17, 26, 100, 142, 1388, 1506, 1956, 3662, 5134
AcoI YGGCCR 3 cut(s) 4009, 4114, 5647
AcuI CTGAAG 8 cut(s) 141, 1199, 1471, 1855, 2264, 2396, 4632, 6243
AcvI CACGTG 4 cut(s) 1508, 3287, 3332, 4886
AcyI GRCGYC 3 cut(s) 3532, 5516, 5526
AdeI CACNNNGTG 2 cut(s) 2898, 3617
AflII CTTAAG 1 cut(s) 994
AflIII ACRYGT 5 cut(s) 1507, 3826, 4447, 4883, 4885
AhlI ACTAGT 2 cut(s) 5746, 6104
AjiI CACGTC 1 cut(s) 3421
AjuI GAANNNNNNNTTGG 8 cut(s) 731, 763, 1451, 1483, 4727, 4759, 5332, 5364
AloI GAACNNNNNNTCC 6 cut(s) 705, 737, 2893, 2925, 3290, 3322
Alw21I GWGCWC 4 cut(s) 1255, 2615, 2894, 3292
Alw26I GTCTC 9 cut(s) 623, 691, 725, 2244, 2829, 3411, 4343, 5003, 5541
Alw44I GTGCAC 1 cut(s) 2890
AlwI GGATC 9 cut(s) 17, 26, 100, 142, 1388, 1506, 1956, 3662, 5134
AlwNI CAGNNNCTG 4 cut(s) 4229, 4994, 5722, 6125
Ama87I CYCGRG 2 cut(s) 5552, 5811
Aor13HI TCCGGA 3 cut(s) 602, 2048, 5101
ApaLI GTGCAC 1 cut(s) 2890
ArsI GACNNNNNNTTYG 6 cut(s) 2774, 2806, 4227, 4259, 5114, 5146
AseI ATTAAT 2 cut(s) 1695, 3168
Asp700I GAANNNNTTC 5 cut(s) 176, 639, 661, 2997, 3363
Asp718I GGTACC 1 cut(s) 3427
AspLEI GCGC 4 cut(s) 898, 4877, 5596, 5920
AsuC2I CCSGG 2 cut(s) 1083, 3265
AsuHPI GGTGA 7 cut(s) 1097, 1505, 1925, 3681, 4723, 5424, 5639
AsuNHI GCTAGC 3 cut(s) 695, 863, 4801
AvaI CYCGRG 2 cut(s) 5552, 5811
AvaII GGWCC 8 cut(s) 311, 1879, 2936, 3053, 3563, 4941, 5050, 5662
BaeGI GKGCMC 2 cut(s) 2894, 4378
BaeI ACNNNNGTAYC 4 cut(s) 3419, 3452, 4770, 4803
BalI TGGCCA 3 cut(s) 4011, 4116, 5649
BanI GGYRCC 1 cut(s) 3427
BanII GRGCYC 1 cut(s) 3513
BauI CACGAG 1 cut(s) 3120
BbrPI CACGTG 4 cut(s) 1508, 3287, 3332, 4886
BbsI GAAGAC 6 cut(s) 178, 1655, 2204, 2949, 3000, 4394
Bbv12I GWGCWC 4 cut(s) 1255, 2615, 2894, 3292
BbvCI CCTCAGC 2 cut(s) 382, 3279
BceAI ACGGC 2 cut(s) 2142, 3915
BcgI CGANNNNNNTGC 6 cut(s) 857, 891, 1628, 1662, 4610, 4644
BciVI GTATCC 1 cut(s) 3207
BclI TGATCA 1 cut(s) 2556
BcnI CCSGG 2 cut(s) 1083, 3265
BcoDI GTCTC 9 cut(s) 623, 691, 725, 2244, 2829, 3411, 4343, 5003, 5541
BcuI ACTAGT 2 cut(s) 5746, 6104
BfmI CTRYAG 4 cut(s) 2417, 4369, 5133, 5723
BfrI CTTAAG 1 cut(s) 994
BfuAI ACCTGC 3 cut(s) 2308, 3338, 3439
BfuI GTATCC 1 cut(s) 3207
BglII AGATCT 1 cut(s) 3410
BmcAI AGTACT 1 cut(s) 3314
Bme18I GGWCC 8 cut(s) 311, 1879, 2936, 3053, 3563, 4941, 5050, 5662
BmeT110I CYCGRG 2 cut(s) 5552, 5811
BmgBI CACGTC 1 cut(s) 3421
BmiI GGNNCC 5 cut(s) 1881, 3429, 3929, 4693, 5615
BmtI GCTAGC 3 cut(s) 699, 867, 4805
BpiI GAAGAC 6 cut(s) 178, 1655, 2204, 2949, 3000, 4394
BpmI CTGGAG 4 cut(s) 589, 1239, 2629, 3435
Bpu10I CCTNAGC 3 cut(s) 382, 3252, 3279
BpuEI CTTGAG 6 cut(s) 1910, 4508, 4699, 4882, 5211, 5811
BpuMI CCSGG 2 cut(s) 1083, 3265
Bsa29I ATCGAT 2 cut(s) 3639, 4962
BsaAI YACGTR 6 cut(s) 1508, 2961, 3287, 3332, 4782, 4886
BsaBI GATNNNNATC 3 cut(s) 97, 139, 2150
BsaHI GRCGYC 3 cut(s) 3532, 5516, 5526
BsaI GGTCTC 2 cut(s) 691, 2829
BsaJI CCNNGG 9 cut(s) 3517, 3578, 3833, 4023, 4204, 4734, 4855, 6018, 6118
BsaWI WCCGGW 3 cut(s) 602, 2048, 5101
BsaXI ACNNNNNCTCC 4 cut(s) 892, 922, 2617, 2647
Bse118I RCCGGY 2 cut(s) 4896, 5475
Bse3DI GCAATG 5 cut(s) 72, 114, 2921, 3532, 4087
Bse8I GATNNNNATC 3 cut(s) 97, 139, 2150
BseAI TCCGGA 3 cut(s) 602, 2048, 5101
BseCI ATCGAT 2 cut(s) 3639, 4962
BseDI CCNNGG 9 cut(s) 3517, 3578, 3833, 4023, 4204, 4734, 4855, 6018, 6118
BseJI GATNNNNATC 3 cut(s) 97, 139, 2150
BseMI GCAATG 5 cut(s) 72, 114, 2921, 3532, 4087
BseRI GAGGAG 6 cut(s) 2679, 4107, 4471, 4919, 4922, 4958
BseSI GKGCMC 2 cut(s) 2894, 4378
BseYI CCCAGC 2 cut(s) 3228, 5888
Bsh1236I CGCG 4 cut(s) 898, 1722, 5568, 5806
Bsh1285I CGRYCG 1 cut(s) 5513
BshNI GGYRCC 1 cut(s) 3427
BshVI ATCGAT 2 cut(s) 3639, 4962
BsiEI CGRYCG 1 cut(s) 5513
BsiHKAI GWGCWC 4 cut(s) 1255, 2615, 2894, 3292
BsiHKCI CYCGRG 2 cut(s) 5552, 5811
BsiSI CCGG 7 cut(s) 603, 1082, 2049, 3265, 4897, 5102, 5476
BsiWI CGTACG 1 cut(s) 5520
BslFI GGGAC 4 cut(s) 1865, 2137, 4461, 5180
BsmAI GTCTC 9 cut(s) 623, 691, 725, 2244, 2829, 3411, 4343, 5003, 5541
BsmBI CGTCTC 3 cut(s) 3411, 5003, 5541
BsmFI GGGAC 4 cut(s) 1865, 2137, 4461, 5180
BsmI GAATGC 2 cut(s) 1235, 3772
Bso31I GGTCTC 2 cut(s) 691, 2829
BsoBI CYCGRG 2 cut(s) 5552, 5811
Bsp1286I GDGCHC 6 cut(s) 1255, 2615, 2894, 3292, 3513, 4378
Bsp13I TCCGGA 3 cut(s) 602, 2048, 5101
Bsp1407I TGTACA 2 cut(s) 3983, 4981
BspDI ATCGAT 2 cut(s) 3639, 4962
BspEI TCCGGA 3 cut(s) 602, 2048, 5101
BspFNI CGCG 4 cut(s) 898, 1722, 5568, 5806
BspHI TCATGA 3 cut(s) 1387, 2779, 2809
BspLI GGNNCC 5 cut(s) 1881, 3429, 3929, 4693, 5615
BspMAI CTGCAG 2 cut(s) 2421, 5727
BspMI ACCTGC 3 cut(s) 2308, 3338, 3439
BspOI GCTAGC 3 cut(s) 699, 867, 4805
BspPI GGATC 9 cut(s) 17, 26, 100, 142, 1388, 1506, 1956, 3662, 5134
BspQI GCTCTTC 1 cut(s) 1218
BspT107I GGYRCC 1 cut(s) 3427
BspTI CTTAAG 1 cut(s) 994
BspTNI GGTCTC 2 cut(s) 691, 2829
BsrBI CCGCTC 1 cut(s) 1894
BsrDI GCAATG 5 cut(s) 72, 114, 2921, 3532, 4087
BsrFI RCCGGY 2 cut(s) 4896, 5475
BsrGI TGTACA 2 cut(s) 3983, 4981
BssAI RCCGGY 2 cut(s) 4896, 5475
BssECI CCNNGG 9 cut(s) 3517, 3578, 3833, 4023, 4204, 4734, 4855, 6018, 6118
BssNAI GTATAC 1 cut(s) 4220
BssNI GRCGYC 3 cut(s) 3532, 5516, 5526
BssSI CACGAG 1 cut(s) 3120
BssT1I CCWWGG 3 cut(s) 4204, 4734, 6018
Bst1107I GTATAC 1 cut(s) 4220
Bst2BI CACGAG 1 cut(s) 3120
Bst6I CTCTTC 7 cut(s) 160, 423, 1164, 1218, 3474, 5484, 5536
BstACI GRCGYC 3 cut(s) 3532, 5516, 5526
BstAFI CTTAAG 1 cut(s) 994
BstAPI GCANNNNNTGC 1 cut(s) 1018
BstAUI TGTACA 2 cut(s) 3983, 4981
BstBAI YACGTR 6 cut(s) 1508, 2961, 3287, 3332, 4782, 4886
BstC8I GCNNGC 8 cut(s) 697, 865, 1637, 1808, 2753, 4803, 4923, 6130
BstDSI CCRYGG 1 cut(s) 3833
BstEII GGTNACC 2 cut(s) 3687, 6022
BstENI CCTNNNNNAGG 1 cut(s) 1401
BstFNI CGCG 4 cut(s) 898, 1722, 5568, 5806
BstHHI GCGC 4 cut(s) 898, 4877, 5596, 5920
BstMAI GTCTC 9 cut(s) 623, 691, 725, 2244, 2829, 3411, 4343, 5003, 5541
BstMCI CGRYCG 1 cut(s) 5513
BstMWI GCNNNNNNNGC 8 cut(s) 383, 1018, 2757, 3269, 3508, 5195, 6174, 6227
BstNSI RCATGY 5 cut(s) 1482, 3830, 4326, 4395, 4451
BstPI GGTNACC 2 cut(s) 3687, 6022
BstSFI CTRYAG 4 cut(s) 2417, 4369, 5133, 5723
BstSLI GKGCMC 2 cut(s) 2894, 4378
BstSNI TACGTA 1 cut(s) 4782
BstUI CGCG 4 cut(s) 898, 1722, 5568, 5806
BstV2I GAAGAC 6 cut(s) 178, 1655, 2204, 2949, 3000, 4394
BstX2I RGATCY 6 cut(s) 92, 134, 1393, 1948, 3410, 5139
BstXI CCANNNNNNTGG 1 cut(s) 5373
BstYI RGATCY 6 cut(s) 92, 134, 1393, 1948, 3410, 5139
BstZ17I GTATAC 1 cut(s) 4220
Bsu15I ATCGAT 2 cut(s) 3639, 4962
BsuI GTATCC 1 cut(s) 3207
BsuTUI ATCGAT 2 cut(s) 3639, 4962
BtgI CCRYGG 1 cut(s) 3833
BtgZI GCGATG 1 cut(s) 1737
BtrI CACGTC 1 cut(s) 3421
BtsI GCAGTG 3 cut(s) 357, 5958, 5971
BveI ACCTGC 3 cut(s) 2308, 3338, 3439
Cac8I GCNNGC 8 cut(s) 697, 865, 1637, 1808, 2753, 4803, 4923, 6130
CaiI CAGNNNCTG 4 cut(s) 4229, 4994, 5722, 6125
CciI TCATGA 3 cut(s) 1387, 2779, 2809
CfoI GCGC 4 cut(s) 898, 4877, 5596, 5920
Cfr10I RCCGGY 2 cut(s) 4896, 5475
ClaI ATCGAT 2 cut(s) 3639, 4962
CseI GACGC 5 cut(s) 2209, 5084, 5453, 5505, 5534
CsiI ACCWGGT 1 cut(s) 5434
DraI TTTAAA 2 cut(s) 2187, 3447
DraIII CACNNNGTG 2 cut(s) 2898, 3617
EaeI YGGCCR 3 cut(s) 4009, 4114, 5647
Eam1104I CTCTTC 7 cut(s) 160, 423, 1164, 1218, 3474, 5484, 5536
EarI CTCTTC 7 cut(s) 160, 423, 1164, 1218, 3474, 5484, 5536
EciI GGCGGA 1 cut(s) 5052
Eco105I TACGTA 1 cut(s) 4782
Eco130I CCWWGG 3 cut(s) 4204, 4734, 6018
Eco147I AGGCCT 2 cut(s) 515, 5824
Eco24I GRGCYC 1 cut(s) 3513
Eco31I GGTCTC 2 cut(s) 691, 2829
Eco32I GATATC 1 cut(s) 2148
Eco47I GGWCC 8 cut(s) 311, 1879, 2936, 3053, 3563, 4941, 5050, 5662
Eco57I CTGAAG 8 cut(s) 141, 1199, 1471, 1855, 2264, 2396, 4632, 6243
Eco72I CACGTG 4 cut(s) 1508, 3287, 3332, 4886
Eco88I CYCGRG 2 cut(s) 5552, 5811
Eco91I GGTNACC 2 cut(s) 3687, 6022
EcoNI CCTNNNNNAGG 1 cut(s) 1401
EcoO109I RGGNCCY 2 cut(s) 5050, 5614
EcoO65I GGTNACC 2 cut(s) 3687, 6022
EcoRI GAATTC 6 cut(s) 261, 989, 2426, 2772, 2877, 5736
EcoRV GATATC 1 cut(s) 2148
EcoT14I CCWWGG 3 cut(s) 4204, 4734, 6018
EcoT22I ATGCAT 3 cut(s) 1614, 3774, 3843
EcoT38I GRGCYC 1 cut(s) 3513
ErhI CCWWGG 3 cut(s) 4204, 4734, 6018
Esp3I CGTCTC 3 cut(s) 3411, 5003, 5541
FalI AAGNNNNNCTT 4 cut(s) 731, 763, 1448, 1480
FaqI GGGAC 4 cut(s) 1865, 2137, 4461, 5180
FauI CCCGC 4 cut(s) 221, 3122, 3917, 6137
FauNDI CATATG 1 cut(s) 766
FbaI TGATCA 1 cut(s) 2556
FblI GTMKAC 4 cut(s) 761, 4219, 5683, 6084
FriOI GRGCYC 1 cut(s) 3513
GlaI GCGC 4 cut(s) 897, 4876, 5595, 5919
GsaI CCCAGC 2 cut(s) 3232, 5892
GsuI CTGGAG 4 cut(s) 589, 1239, 2629, 3435
HapII CCGG 7 cut(s) 603, 1082, 2049, 3265, 4897, 5102, 5476
HgaI GACGC 5 cut(s) 2209, 5084, 5453, 5505, 5534
HhaI GCGC 4 cut(s) 898, 4877, 5596, 5920
Hin1I GRCGYC 3 cut(s) 3532, 5516, 5526
Hin6I GCGC 4 cut(s) 896, 4875, 5594, 5918
HinP1I GCGC 4 cut(s) 896, 4875, 5594, 5918
HincII GTYRAC 4 cut(s) 1152, 2008, 3858, 5752
HindII GTYRAC 4 cut(s) 1152, 2008, 3858, 5752
HindIII AAGCTT 2 cut(s) 576, 1062
HpaII CCGG 7 cut(s) 603, 1082, 2049, 3265, 4897, 5102, 5476
HphI GGTGA 7 cut(s) 1097, 1505, 1925, 3681, 4723, 5424, 5639
Hpy99I CGWCG 7 cut(s) 2225, 3047, 4545, 4977, 5521, 5528, 5573
HpyF10VI GCNNNNNNNGC 8 cut(s) 383, 1018, 2757, 3269, 3508, 5195, 6174, 6227
Hsp92I GRCGYC 3 cut(s) 3532, 5516, 5526
HspAI GCGC 4 cut(s) 896, 4875, 5594, 5918
Kpn2I TCCGGA 3 cut(s) 602, 2048, 5101
KpnI GGTACC 1 cut(s) 3431
Ksp22I TGATCA 1 cut(s) 2556
LguI GCTCTTC 1 cut(s) 1218
LmnI GCTCC 7 cut(s) 1258, 2137, 2596, 2610, 3244, 3927, 5948
MabI ACCWGGT 1 cut(s) 5434
MbiI CCGCTC 1 cut(s) 1894
MfeI CAATTG 6 cut(s) 1941, 2159, 3903, 4005, 5216, 5937
MflI RGATCY 6 cut(s) 92, 134, 1393, 1948, 3410, 5139
MhlI GDGCHC 6 cut(s) 1255, 2615, 2894, 3292, 3513, 4378
MlsI TGGCCA 3 cut(s) 4011, 4116, 5649
MluNI TGGCCA 3 cut(s) 4011, 4116, 5649
MmeI TCCRAC 9 cut(s) 174, 493, 691, 1302, 1347, 2065, 2179, 3366, 6037
Mox20I TGGCCA 3 cut(s) 4011, 4116, 5649
Mph1103I ATGCAT 3 cut(s) 1614, 3774, 3843
MroI TCCGGA 3 cut(s) 602, 2048, 5101
MroXI GAANNNNTTC 5 cut(s) 176, 639, 661, 2997, 3363
MscI TGGCCA 3 cut(s) 4011, 4116, 5649
MslI CAYNNNNRTG 2 cut(s) 2467, 5121
Msp20I TGGCCA 3 cut(s) 4011, 4116, 5649
MspA1I CMGCKG 2 cut(s) 3148, 6230
MspCI CTTAAG 1 cut(s) 994
MspI CCGG 7 cut(s) 603, 1082, 2049, 3265, 4897, 5102, 5476
MunI CAATTG 6 cut(s) 1941, 2159, 3903, 4005, 5216, 5937
Mva1269I GAATGC 2 cut(s) 1235, 3772
MvnI CGCG 4 cut(s) 898, 1722, 5568, 5806
MwoI GCNNNNNNNGC 8 cut(s) 383, 1018, 2757, 3269, 3508, 5195, 6174, 6227
NciI CCSGG 2 cut(s) 1083, 3265
NdeI CATATG 1 cut(s) 766
NheI GCTAGC 3 cut(s) 695, 863, 4801
NlaIV GGNNCC 5 cut(s) 1881, 3429, 3929, 4693, 5615
NmeAIII GCCGAG 1 cut(s) 5013
NmuCI GTSAC 5 cut(s) 2678, 3093, 3687, 3707, 4673
NsiI ATGCAT 3 cut(s) 1614, 3774, 3843
NspI RCATGY 5 cut(s) 1482, 3830, 4326, 4395, 4451
PaeR7I CTCGAG 1 cut(s) 5552
PagI TCATGA 3 cut(s) 1387, 2779, 2809
PaqCI CACCTGC 2 cut(s) 2308, 3338
PceI AGGCCT 2 cut(s) 515, 5824
PciI ACATGT 2 cut(s) 3826, 4447
PciSI GCTCTTC 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 3 cut(s) 3007, 4546, 5550
PctI GAATGC 2 cut(s) 1235, 3772
PdmI GAANNNNTTC 5 cut(s) 176, 639, 661, 2997, 3363
Pfl23II CGTACG 1 cut(s) 5520
PflFI GACNNNGTC 1 cut(s) 5505
PflMI CCANNNNNTGG 4 cut(s) 1943, 3698, 4229, 5159
PfoI TCCNGGA 1 cut(s) 2401
Ple19I CGATCG 1 cut(s) 5513
PmaCI CACGTG 4 cut(s) 1508, 3287, 3332, 4886
PmlI CACGTG 4 cut(s) 1508, 3287, 3332, 4886
Ppu21I YACGTR 6 cut(s) 1508, 2961, 3287, 3332, 4782, 4886
PpuMI RGGWCCY 1 cut(s) 5050
PscI ACATGT 2 cut(s) 3826, 4447
PshBI ATTAAT 2 cut(s) 1695, 3168
PsiI TTATAA 3 cut(s) 1124, 1266, 1572
Psp1406I AACGTT 1 cut(s) 2036
Psp5II RGGWCCY 1 cut(s) 5050
PspCI CACGTG 4 cut(s) 1508, 3287, 3332, 4886
PspEI GGTNACC 2 cut(s) 3687, 6022
PspFI CCCAGC 2 cut(s) 3228, 5888
PspLI CGTACG 1 cut(s) 5520
PspN4I GGNNCC 5 cut(s) 1881, 3429, 3929, 4693, 5615
PspPPI RGGWCCY 1 cut(s) 5050
PstI CTGCAG 2 cut(s) 2421, 5727
PstNI CAGNNNCTG 4 cut(s) 4229, 4994, 5722, 6125
PsuI RGATCY 6 cut(s) 92, 134, 1393, 1948, 3410, 5139
PsyI GACNNNGTC 1 cut(s) 5505
PvuI CGATCG 1 cut(s) 5513
PvuII CAGCTG 2 cut(s) 3148, 6230
RseI CAYNNNNRTG 2 cut(s) 2467, 5121
SapI GCTCTTC 1 cut(s) 1218
ScaI AGTACT 1 cut(s) 3314
SduI GDGCHC 6 cut(s) 1255, 2615, 2894, 3292, 3513, 4378
SexAI ACCWGGT 1 cut(s) 5434
SfcI CTRYAG 4 cut(s) 2417, 4369, 5133, 5723
Sfr274I CTCGAG 1 cut(s) 5552
SinI GGWCC 8 cut(s) 311, 1879, 2936, 3053, 3563, 4941, 5050, 5662
SlaI CTCGAG 1 cut(s) 5552
SmiMI CAYNNNNRTG 2 cut(s) 2467, 5121
SmlI CTYRAG 8 cut(s) 994, 1889, 4523, 4678, 4861, 5226, 5552, 5826
SmoI CTYRAG 8 cut(s) 994, 1889, 4523, 4678, 4861, 5226, 5552, 5826
SnaBI TACGTA 1 cut(s) 4782
SpeI ACTAGT 2 cut(s) 5746, 6104
SseBI AGGCCT 2 cut(s) 515, 5824
SspI AATATT 3 cut(s) 2717, 4179, 4415
StuI AGGCCT 2 cut(s) 515, 5824
StyI CCWWGG 3 cut(s) 4204, 4734, 6018
TatI WGTACW 8 cut(s) 237, 1162, 1756, 2843, 3312, 3820, 3983, 4981
TauI GCSGC 4 cut(s) 1897, 5589, 5785, 5806
TseFI GTSAC 5 cut(s) 2678, 3093, 3687, 3707, 4673
Tsp45I GTSAC 5 cut(s) 2678, 3093, 3687, 3707, 4673
TspGWI ACGGA 8 cut(s) 555, 566, 961, 1757, 2627, 3850, 4751, 4961
Tth111I GACNNNGTC 1 cut(s) 5505
Van91I CCANNNNNTGG 4 cut(s) 1943, 3698, 4229, 5159
Vha464I CTTAAG 1 cut(s) 994
VneI GTGCAC 1 cut(s) 2890
VpaK11BI GGWCC 8 cut(s) 311, 1879, 2936, 3053, 3563, 4941, 5050, 5662
VspI ATTAAT 2 cut(s) 1695, 3168
XagI CCTNNNNNAGG 1 cut(s) 1401
XbaI TCTAGA 1 cut(s) 1993
XceI RCATGY 5 cut(s) 1482, 3830, 4326, 4395, 4451
XcmI CCANNNNNNNNNTGG 4 cut(s) 1152, 2162, 3524, 4639
XhoI CTCGAG 1 cut(s) 5552
XmiI GTMKAC 4 cut(s) 761, 4219, 5683, 6084
XmnI GAANNNNTTC 5 cut(s) 176, 639, 661, 2997, 3363
ZraI GACGTC 1 cut(s) 3533
ZrmI AGTACT 1 cut(s) 3314
Zsp2I ATGCAT 3 cut(s) 1614, 3774, 3843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.