RchiOBHm_Chr2g0106571

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000307
Physical Location & Seq
Reverse (-)
17932781 .. 17940267
7487 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48065

Sequence Viewer

Length: 3174 bp
ATGGAACCACCTTCATCATCTTCCTCCCCCAACCCCCAAACAGTTCCGTCATCCTCTAACTGGGAACATGATGTCTTCCTCAGTTTCAGGGGCGAGGACACTCGCAATAGTTTTACAGACCATTTATACTATGCTATGAGCCAGAGAGGAATCGACACATTTAGAGATACTGAAAAGCTTCGGAGGGGGAAATCCATTTCACCAGAACTTCTGAAAGCAATCGAAGAGTCCAAGTTTGCAGTTACAGTTCTTTCAACCAACTATGCTACTTCAACTTGGTGTCTGGATGAACTTGCCCATATTCTCGATTGCAGGAAAGAAAGGGGACTGGAAGTTCTTCCGGTTTTTTATCATGTAGAACCATCTGAGATAAGGAAGCAAACTGGAAACTACGGCAAGGCGTTTGCTAAGCATGAGACAGATTTCAAGGAAAATATGAGAAAGGTGGACAAGTGGAGGAAAGCTTTAGAAGATATAGCCAGTCTCTCCGGATGGCATGTGACACAGGATAGGGGAGAATCGGAAGTTATACAGGAAATAGCTAAAGAGATTTCAATATGTTTGAACAACACATTATCCAATCCTGACAGAGATTTGATTGGAATGGATGTGCGTATAGAAAAGTTGGAGTCTTACTTGGATCTAAGGTCGGATGACGTTCTTAGCATAGGGATTTGGGGGATGGGGGGCATTGGTAAGACAACTCTGGCAAAAGAAGTTTTCAAAAAGATTCGTAACCAATTTGATAGGAGCGGCTTTCTTTCCAATGTTAGATTGCATTCCGAAGAAAGAGCTCTAGTTGAGTTGCAAAAACTTCTGTGTGGATCCTCTTTCGAGAACAACAACATAAATATAGACACTGTTGGAGAAGGGATCAGGTTGTTAAAGAAAAAATTACTTAAGAAAAAGGTGCTTATTGTTCTAGATGATGTTGATGAATTAAAACAACTGAAAGATTTAGCACCTGGAAGGCAGAATGAACAGTACTCTTGGGGCCGAGGGAGTCGACTCATAATAACAACTAGGGATAGGCGTCCATTGATAGAATGTGAAGTACATAAAATATATGAGGCTGAAAAACTTAGTGATGAGGAAGCTTCTCAGCTCATATGTCAACAAGCCTTCAAGAAAAACTATCCACCAGCTGAGTTTGTAGAGCTGTCCAAGAGTTTTGTAAAATATGCCGGTGGCCTTCCTCTAGCTCATAAAGTTAAGGGTTCACAGTTGTGGGGAAGGGAGGTAGATGAATGGTCAGAGGTATTAGATAGACTGGACGAAGATCTGGATAAAGACATTTTTAGTGTGCTTGAAATAAGTTTTGATGAATTACAGGATACAGATAAGAAAATCTTTTTGGACATCGCATGTTTCTTCAATGGTGAGGATCATGTTCGTGTACAAAAGATATTGAAAAGTTGTGGTTTTTCTCCTCGAATAGGCATAACTAACCTCATTGACAAATCTCTGATCAAAATTGAAAGTGAAAAACTGTGGATGCATGAATTACTACGGTGTTTGGGTTGGCATATTGTTCGCAGAGAATCAAAACCTTTTCCGGGCAAACGTAGCAGATTGTGGCTTGATGATAATGCACACAAGTACAAAGGCAGAAGGCCATGGCACTTTGAGGATGCTCGTAATGTACTCGCGAGTAATACCGGAACATCTGCTGTTGAAGGCTTATTCTTAAGCTTGGGTGAAAAAGAAGAAATGCGTTTGAGCAATGACCCGTTCTTAACTATGACCAAACTAAGATTGCTGAAGATTTATAATGTAAATTTTCTGGGCGGGCACTTTACATATCTCTCTAACGATTTACGTTGTCTGGAATGGCACGAATGTCCTCTAAAGTCTCTGCCATCTGGTTTTACGCCCTATCAGCTGGTTGAATTCAAGATGCCTAACAGCTGCATTGAACGACTGTGGAAGAAAACGCCTTCTATGAGAATGCTGACGCTCATGGATCTTTCCAACTGCCAATATCTAGTCACGACCCCTGATTTCAGTGAGGTCCCAAATCTTGAGAGATTGATCCTTGAAGGTTGTAAAGAGTTATCTGCGGTTCACCCAACAATTAGGGATCTCCAGCATCTGATTTTATTGAATTTGAAAGGCTGTGCAAGTCTAAAGAGCCTTCCCCCATCAATCAGCTTGAGATCTCTTCAAACTTTTATTCTTTCGGGATGTTCAAAACTGGAAGAGTTTCCGGAGATTGTGGGAAACATGCAAACTTTGTCAGAACTTTATTTAGATGGCACCGCTATAAGGGAGGTGCCTGTATCAATCCAGCTTTTAACAGGCCTTGTTTTGCTAAATCTATGTGGCTGCAAGAACCTTCCGAGTCTTCCAAGCGCTCTTTGTAGAAGTTTGACATCACTGAAATTTCTTTATTTGTCATGCTGCTCTAGTTTGGACAAACTGCCAGAAAACATAGGCTCCTTGGAACACTTGGAGGAGCTTGATGCATGTTATACTGCTATAAGAAAAGTGCCTGAGTCCATTTCACTACTCAAGAATCTTAAACTACTGTGTTTCCATGGTTGCTCTGGATACACAGGTCTAGAGATGCCAAACAAGTTCTCAGGTCTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCCCAGAAGGAGCAATCCCCAGTGACATTGGCAATTTGTTCTCACTGCAGAGTCTGGATTTAAGTGAAAACAACTTTTTCACCATTCCTGAAAGCATCTCTCAACTTTCTGAGCTTACAGAAATTTTTCTGTTTAGGTGTAGCAAACTACAATCGTTGCCAAAAGATCTTCCATCAAGACTAAGAACTATAAATTTACGGGACTGTCCTTTGCTGACAGATTCTTCATATAATTCGATGAGATATCCACCTCGAAAGGGTTTAAGTACTATCATAAGTTGTCGAAAACCGGAGGAGGATGAGCAGTTGCCAATATTACTATCTGAACTCCATGAGGTGTCTCTTTCTCTGCAGTTGCCAATGCTACCTGAACTCCATGAGTTTCGTTTGGGAAATCTGGAAAAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAACCTTAAGAAATTGATCCTTGAAGGTTGTGAAAAATTATCAGAGGTTCACCCAACATTTGGGAGTCTCCAACATCTGATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

1057

Amino Acids

120.23

Weight (kDa)

6.21

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 194 4.4e-56 TIR domain
TIR_2 PF13676 25 - 122 1.1e-15 TIR domain
NB-ARC PF00931 206 - 378 9.1e-20 NB-ARC domain
NACHT PF05729 225 - 372 1.7e-06 NACHT domain
WHD_ROQ1 PF23282 446 - 515 8.2e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 718 - 786 7.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 730 - 795 9e-06 Leucine-rich repeat region
LRR_14 PF23598 770 - 871 2.2e-12 Leucine-rich repeat region
LRR_13 PF23286 804 - 948 1.3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1770
AccB1I GGYRCC 2 cut(s) 2255, 2272
AccB7I CCANNNNNTGG 1 cut(s) 3146
AccBSI CCGCTC 1 cut(s) 753
AccI GTMKAC 1 cut(s) 1006
AccII CGCG 1 cut(s) 1649
AccIII TCCGGA 2 cut(s) 488, 2206
AciI CCGC 4 cut(s) 753, 1788, 2060, 2259
AcsI RAATTY 6 cut(s) 1777, 1889, 2104, 2381, 2748, 2818
AcuI CTGAAG 1 cut(s) 1781
AcyI GRCGYC 1 cut(s) 1033
AfaI GTAC 6 cut(s) 986, 1056, 1398, 1601, 1644, 2893
AfeI AGCGCT 1 cut(s) 2353
AfiI CCNNNNNNNGG 6 cut(s) 60, 1436, 1556, 2265, 2882, 3146
AflII CTTAAG 3 cut(s) 899, 1687, 3092
AjnI CCWGG 1 cut(s) 964
AjuI GAANNNNNNNTTGG 2 cut(s) 1337, 1369
AleI CACNNNNGTG 1 cut(s) 1225
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
Alw21I GWGCWC 1 cut(s) 796
Alw26I GTCTC 5 cut(s) 410, 488, 1857, 2970, 3158
AlwNI CAGNNNCTG 2 cut(s) 2092, 2680
Aor13HI TCCGGA 2 cut(s) 488, 2206
Aor51HI AGCGCT 1 cut(s) 2353
AoxI GGCC 4 cut(s) 994, 1189, 1613, 2299
ApeKI GCWGC 3 cut(s) 1908, 2325, 2400
ApoI RAATTY 6 cut(s) 1777, 1889, 2104, 2381, 2748, 2818
Asp700I GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
AspA2I CCTAGG 1 cut(s) 2611
AspLEI GCGC 1 cut(s) 2354
AspS9I GGNCC 2 cut(s) 994, 2011
AsuC2I CCSGG 1 cut(s) 1557
AsuHPI GGTGA 6 cut(s) 192, 1391, 1709, 2057, 2698, 3128
AvaII GGWCC 1 cut(s) 2011
AvrII CCTAGG 1 cut(s) 2611
BaeGI GKGCMC 1 cut(s) 1794
BaeI ACNNNNGTAYC 2 cut(s) 2542, 2575
BamHI GGATCC 1 cut(s) 824
BanI GGYRCC 2 cut(s) 2255, 2272
BanII GRGCYC 1 cut(s) 796
BbsI GAAGAC 2 cut(s) 67, 2336
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 3 cut(s) 1895, 2312, 2387
BccI CCATC 7 cut(s) 370, 486, 676, 1867, 2149, 2246, 2806
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 2 cut(s) 1514, 1548
BciT130I CCWGG 1 cut(s) 966
BciVI GTATCC 2 cut(s) 1327, 2543
BclI TGATCA 1 cut(s) 1467
BcnI CCSGG 1 cut(s) 1557
BcoDI GTCTC 5 cut(s) 410, 488, 1857, 2970, 3158
BfaI CTAG 8 cut(s) 797, 923, 1023, 1199, 1985, 2406, 2561, 2612
BfmI CTRYAG 2 cut(s) 2672, 2975
BfoI RGCGCY 1 cut(s) 2355
BfrI CTTAAG 3 cut(s) 899, 1687, 3092
BfuI GTATCC 2 cut(s) 1327, 2543
BglII AGATCT 4 cut(s) 1279, 2156, 2591, 2791
BisI GCNGC 4 cut(s) 754, 1909, 2326, 2401
BlnI CCTAGG 1 cut(s) 2611
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 4 cut(s) 755, 1910, 2327, 2402
BmcAI AGTACT 2 cut(s) 986, 2893
Bme1390I CCNGG 2 cut(s) 966, 1557
Bme18I GGWCC 1 cut(s) 2011
BmgT120I GGNCC 2 cut(s) 994, 2011
BmiI GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
BmrFI CCNGG 2 cut(s) 966, 1557
BmrI ACTGGG 2 cut(s) 70, 2640
BmsI GCATC 7 cut(s) 1485, 1621, 1887, 2098, 2452, 2556, 2730
BmuI ACTGGG 2 cut(s) 70, 2640
BpiI GAAGAC 2 cut(s) 67, 2336
BpmI CTGGAG 1 cut(s) 2069
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 3 cut(s) 2042, 2173, 2495
BpuMI CCSGG 1 cut(s) 1557
BsaHI GRCGYC 1 cut(s) 1033
BsaJI CCNNGG 5 cut(s) 997, 1616, 2439, 2536, 2611
BsaWI WCCGGW 5 cut(s) 340, 488, 1658, 2206, 2914
BsaXI ACNNNNNCTCC 4 cut(s) 2420, 2450, 2982, 3012
Bsc4I CCNNNNNNNGG 6 cut(s) 60, 1436, 1556, 2265, 2882, 3146
Bse118I RCCGGY 1 cut(s) 1184
Bse1I ACTGG 7 cut(s) 65, 333, 388, 480, 1275, 2199, 2646
Bse3DI GCAATG 1 cut(s) 1729
BseAI TCCGGA 2 cut(s) 488, 2206
BseBI CCWGG 1 cut(s) 966
BseDI CCNNGG 5 cut(s) 997, 1616, 2439, 2536, 2611
BseLI CCNNNNNNNGG 6 cut(s) 60, 1436, 1556, 2265, 2882, 3146
BseMI GCAATG 1 cut(s) 1729
BseMII CTCAG 7 cut(s) 94, 357, 1115, 1137, 2484, 2595, 2727
BseNI ACTGG 7 cut(s) 65, 333, 388, 480, 1275, 2199, 2646
BseRI GAGGAG 3 cut(s) 1419, 2468, 2933
BseSI GKGCMC 1 cut(s) 1794
BseXI GCAGC 3 cut(s) 1895, 2312, 2387
Bsh1236I CGCG 1 cut(s) 1649
BshFI GGCC 4 cut(s) 996, 1191, 1615, 2301
BshNI GGYRCC 2 cut(s) 2255, 2272
BsiHKAI GWGCWC 1 cut(s) 796
BsiSI CCGG 7 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915
BslFI GGGAC 3 cut(s) 339, 1997, 2840
BslI CCNNNNNNNGG 6 cut(s) 60, 1436, 1556, 2265, 2882, 3146
BsmAI GTCTC 5 cut(s) 410, 488, 1857, 2970, 3158
BsmFI GGGAC 3 cut(s) 339, 1997, 2840
BsmI GAATGC 2 cut(s) 778, 1953
BsnI GGCC 4 cut(s) 996, 1191, 1615, 2301
Bsp1286I GDGCHC 2 cut(s) 796, 1794
Bsp13I TCCGGA 2 cut(s) 488, 2206
Bsp1407I TGTACA 1 cut(s) 1396
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 2 cut(s) 1616, 2536
Bsp68I TCGCGA 1 cut(s) 1649
BspACI CCGC 4 cut(s) 753, 1788, 2060, 2259
BspANI GGCC 4 cut(s) 996, 1191, 1615, 2301
BspCNI CTCAG 7 cut(s) 93, 358, 1114, 1138, 2485, 2594, 2728
BspEI TCCGGA 2 cut(s) 488, 2206
BspFNI CGCG 1 cut(s) 1649
BspLI GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
BspMAI CTGCAG 2 cut(s) 2676, 2979
BspT107I GGYRCC 2 cut(s) 2255, 2272
BspTI CTTAAG 3 cut(s) 899, 1687, 3092
BsrBI CCGCTC 1 cut(s) 753
BsrDI GCAATG 1 cut(s) 1729
BsrFI RCCGGY 1 cut(s) 1184
BsrGI TGTACA 1 cut(s) 1396
BsrI ACTGG 7 cut(s) 65, 333, 388, 480, 1275, 2199, 2646
BssAI RCCGGY 1 cut(s) 1184
BssECI CCNNGG 5 cut(s) 997, 1616, 2439, 2536, 2611
BssNI GRCGYC 1 cut(s) 1033
BssT1I CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
Bst2UI CCWGG 1 cut(s) 966
Bst6I CTCTTC 3 cut(s) 219, 2166, 2193
BstACI GRCGYC 1 cut(s) 1033
BstAFI CTTAAG 3 cut(s) 899, 1687, 3092
BstAUI TGTACA 1 cut(s) 1396
BstC8I GCNNGC 1 cut(s) 1790
BstDSI CCRYGG 2 cut(s) 1616, 2536
BstENI CCTNNNNNAGG 1 cut(s) 1434
BstFNI CGCG 1 cut(s) 1649
BstH2I RGCGCY 1 cut(s) 2355
BstHHI GCGC 1 cut(s) 2354
BstMAI GTCTC 5 cut(s) 410, 488, 1857, 2970, 3158
BstMWI GCNNNNNNNGC 2 cut(s) 1566, 1879
BstNI CCWGG 1 cut(s) 966
BstNSI RCATGY 4 cut(s) 500, 1368, 2227, 2469
BstSCI CCNGG 2 cut(s) 964, 1555
BstSFI CTRYAG 2 cut(s) 2672, 2975
BstSLI GKGCMC 1 cut(s) 1794
BstUI CGCG 1 cut(s) 1649
BstV1I GCAGC 3 cut(s) 1895, 2312, 2387
BstV2I GAAGAC 2 cut(s) 67, 2336
BstX2I RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2591, 2791, 3063
BstYI RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2591, 2791, 3063
BsuI GTATCC 2 cut(s) 1327, 2543
BsuRI GGCC 4 cut(s) 996, 1191, 1615, 2301
BtgI CCRYGG 2 cut(s) 1616, 2536
BtgZI GCGATG 1 cut(s) 1345
BtsI GCAGTG 1 cut(s) 2669
BtsIMutI CAGTG 5 cut(s) 858, 2011, 2375, 2653, 2669
BtuMI TCGCGA 1 cut(s) 1649
Cac8I GCNNGC 1 cut(s) 1790
CaiI CAGNNNCTG 2 cut(s) 2092, 2680
CfoI GCGC 1 cut(s) 2354
Cfr10I RCCGGY 1 cut(s) 1184
Cfr13I GGNCC 2 cut(s) 994, 2011
CseI GACGC 2 cut(s) 1022, 1963
Csp6I GTAC 6 cut(s) 985, 1055, 1397, 1600, 1643, 2892
CviQI GTAC 6 cut(s) 985, 1055, 1397, 1600, 1643, 2892
Eam1104I CTCTTC 3 cut(s) 219, 2166, 2193
EarI CTCTTC 3 cut(s) 219, 2166, 2193
Ecl136II GAGCTC 1 cut(s) 794
Eco130I CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
Eco147I AGGCCT 1 cut(s) 2301
Eco24I GRGCYC 1 cut(s) 796
Eco32I GATATC 1 cut(s) 2870
Eco47I GGWCC 1 cut(s) 2011
Eco47III AGCGCT 1 cut(s) 2353
Eco53kI GAGCTC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 1781
EcoICRI GAGCTC 1 cut(s) 794
EcoNI CCTNNNNNAGG 1 cut(s) 1434
EcoO109I RGGNCCY 1 cut(s) 2011
EcoRI GAATTC 1 cut(s) 1889
EcoRII CCWGG 1 cut(s) 964
EcoRV GATATC 1 cut(s) 2870
EcoT14I CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
EcoT22I ATGCAT 2 cut(s) 1500, 2467
EcoT38I GRGCYC 1 cut(s) 796
ErhI CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
FalI AAGNNNNNCTT 2 cut(s) 1334, 1366
FaqI GGGAC 3 cut(s) 339, 1997, 2840
FauI CCCGC 1 cut(s) 1781
FauNDI CATATG 1 cut(s) 1109
FbaI TGATCA 1 cut(s) 1467
FblI GTMKAC 1 cut(s) 1006
Fnu4HI GCNGC 4 cut(s) 754, 1909, 2326, 2401
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 4 cut(s) 754, 1909, 2326, 2401
FspBI CTAG 8 cut(s) 797, 923, 1023, 1199, 1985, 2406, 2561, 2612
GlaI GCGC 1 cut(s) 2353
GluI GCNGC 4 cut(s) 754, 1909, 2326, 2401
GsuI CTGGAG 1 cut(s) 2069
HaeII RGCGCY 1 cut(s) 2355
HaeIII GGCC 4 cut(s) 996, 1191, 1615, 2301
HapII CCGG 7 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915
HgaI GACGC 2 cut(s) 1022, 1963
HhaI GCGC 1 cut(s) 2354
Hin1I GRCGYC 1 cut(s) 1033
Hin6I GCGC 1 cut(s) 2352
HinP1I GCGC 1 cut(s) 2352
HincII GTYRAC 2 cut(s) 1007, 1115
HindII GTYRAC 2 cut(s) 1007, 1115
HindIII AAGCTT 4 cut(s) 176, 462, 1095, 1690
HpaII CCGG 7 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915
HphI GGTGA 6 cut(s) 192, 1391, 1709, 2057, 2698, 3128
Hpy166II GTNNAC 7 cut(s) 448, 1007, 1115, 1220, 1397, 2065, 3136
Hpy8I GTNNAC 7 cut(s) 448, 1007, 1115, 1220, 1397, 2065, 3136
HpyCH4IV ACGT 3 cut(s) 657, 1564, 1819
HpyF10VI GCNNNNNNNGC 2 cut(s) 1566, 1879
HpySE526I ACGT 3 cut(s) 657, 1564, 1819
Hsp92I GRCGYC 1 cut(s) 1033
HspAI GCGC 1 cut(s) 2352
Kpn2I TCCGGA 2 cut(s) 488, 2206
Ksp22I TGATCA 1 cut(s) 1467
LmnI GCTCC 4 cut(s) 750, 2441, 2455, 2636
Lsp1109I GCAGC 3 cut(s) 1895, 2312, 2387
LweI GCATC 7 cut(s) 1485, 1621, 1887, 2098, 2452, 2556, 2730
MaeI CTAG 8 cut(s) 797, 923, 1023, 1199, 1985, 2406, 2561, 2612
MaeII ACGT 3 cut(s) 657, 1564, 1819
MaeIII GTNAC 5 cut(s) 241, 499, 734, 1987, 2648
MbiI CCGCTC 1 cut(s) 753
MflI RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2591, 2791, 3063
MhlI GDGCHC 2 cut(s) 796, 1794
MlyI GAGTC 8 cut(s) 236, 638, 1002, 1012, 2350, 2504, 2686, 3160
MmeI TCCRAC 4 cut(s) 606, 630, 844, 1995
Mph1103I ATGCAT 2 cut(s) 1500, 2467
MroI TCCGGA 2 cut(s) 488, 2206
MroXI GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
MslI CAYNNNNRTG 2 cut(s) 1225, 1392
MspA1I CMGCKG 3 cut(s) 1145, 1882, 1908
MspCI CTTAAG 3 cut(s) 899, 1687, 3092
MspI CCGG 7 cut(s) 341, 489, 1185, 1556, 1659, 2207, 2915
MspR9I CCNGG 2 cut(s) 966, 1557
Mva1269I GAATGC 2 cut(s) 778, 1953
MvaI CCWGG 1 cut(s) 966
MvnI CGCG 1 cut(s) 1649
MwoI GCNNNNNNNGC 2 cut(s) 1566, 1879
NciI CCSGG 1 cut(s) 1557
NcoI CCATGG 2 cut(s) 1616, 2536
NdeI CATATG 1 cut(s) 1109
NlaIV GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
NmeAIII GCCGAG 1 cut(s) 1022
NmuCI GTSAC 3 cut(s) 499, 1987, 2648
NruI TCGCGA 1 cut(s) 1649
NsiI ATGCAT 2 cut(s) 1500, 2467
NspI RCATGY 4 cut(s) 500, 1368, 2227, 2469
OliI CACNNNNGTG 1 cut(s) 1225
PceI AGGCCT 1 cut(s) 2301
PctI GAATGC 2 cut(s) 778, 1953
PdmI GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
PfeI GAWTC 6 cut(s) 150, 518, 730, 1541, 2515, 2846
PflMI CCANNNNNTGG 1 cut(s) 3146
PkrI GCNGC 4 cut(s) 755, 1910, 2327, 2402
PleI GAGTC 8 cut(s) 235, 637, 1002, 1011, 2349, 2503, 2685, 3159
PpsI GAGTC 8 cut(s) 235, 637, 1002, 1011, 2349, 2503, 2685, 3159
PpuMI RGGWCCY 1 cut(s) 2011
PsiI TTATAA 1 cut(s) 1770
Psp124BI GAGCTC 1 cut(s) 796
Psp5II RGGWCCY 1 cut(s) 2011
Psp6I CCWGG 1 cut(s) 964
PspGI CCWGG 1 cut(s) 964
PspN4I GGNNCC 8 cut(s) 6, 826, 995, 2013, 2257, 2274, 2437, 3084
PspPI GGNCC 2 cut(s) 994, 2011
PspPPI RGGWCCY 1 cut(s) 2011
PstI CTGCAG 2 cut(s) 2676, 2979
PstNI CAGNNNCTG 2 cut(s) 2092, 2680
PsuI RGATCY 9 cut(s) 640, 824, 1279, 1963, 2080, 2156, 2591, 2791, 3063
PvuII CAGCTG 3 cut(s) 1145, 1882, 1908
RruI TCGCGA 1 cut(s) 1649
RsaI GTAC 6 cut(s) 986, 1056, 1398, 1601, 1644, 2893
RsaNI GTAC 6 cut(s) 985, 1055, 1397, 1600, 1643, 2892
RseI CAYNNNNRTG 2 cut(s) 1225, 1392
SacI GAGCTC 1 cut(s) 796
SalI GTCGAC 1 cut(s) 1005
SatI GCNGC 4 cut(s) 754, 1909, 2326, 2401
Sau96I GGNCC 2 cut(s) 994, 2011
ScaI AGTACT 2 cut(s) 986, 2893
SchI GAGTC 8 cut(s) 236, 638, 1002, 1012, 2350, 2504, 2686, 3160
ScrFI CCNGG 2 cut(s) 966, 1557
SduI GDGCHC 2 cut(s) 796, 1794
SfaNI GCATC 7 cut(s) 1485, 1621, 1887, 2098, 2452, 2556, 2730
SfcI CTRYAG 2 cut(s) 2672, 2975
SinI GGWCC 1 cut(s) 2011
SmiMI CAYNNNNRTG 2 cut(s) 1225, 1392
SmlI CTYRAG 6 cut(s) 899, 1687, 2021, 2152, 2510, 3092
SmoI CTYRAG 6 cut(s) 899, 1687, 2021, 2152, 2510, 3092
SseBI AGGCCT 1 cut(s) 2301
SsiI CCGC 4 cut(s) 753, 1788, 2060, 2259
SspI AATATT 1 cut(s) 2940
SspMI CTAG 8 cut(s) 797, 923, 1023, 1199, 1985, 2406, 2561, 2612
SstI GAGCTC 1 cut(s) 796
StuI AGGCCT 1 cut(s) 2301
StyD4I CCNGG 2 cut(s) 964, 1555
StyI CCWWGG 4 cut(s) 1616, 2439, 2536, 2611
TaiI ACGT 3 cut(s) 660, 1567, 1822
TatI WGTACW 6 cut(s) 984, 1054, 1396, 1599, 1642, 2891
TauI GCSGC 1 cut(s) 756
TfiI GAWTC 6 cut(s) 150, 518, 730, 1541, 2515, 2846
TscAI CASTG 5 cut(s) 865, 2011, 2382, 2653, 2676
TseFI GTSAC 3 cut(s) 499, 1987, 2648
TseI GCWGC 3 cut(s) 1908, 2325, 2400
Tsp45I GTSAC 3 cut(s) 499, 1987, 2648
TspDTI ATGAA 7 cut(s) 303, 951, 993, 1260, 1338, 1515, 2841
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 5 cut(s) 865, 2011, 2382, 2653, 2676
Van91I CCANNNNNTGG 1 cut(s) 3146
Vha464I CTTAAG 3 cut(s) 899, 1687, 3092
VpaK11BI GGWCC 1 cut(s) 2011
XagI CCTNNNNNAGG 1 cut(s) 1434
XapI RAATTY 6 cut(s) 1777, 1889, 2104, 2381, 2748, 2818
XbaI TCTAGA 2 cut(s) 922, 2560
XceI RCATGY 4 cut(s) 500, 1368, 2227, 2469
XcmI CCANNNNNNNNNTGG 1 cut(s) 2543
XmaJI CCTAGG 1 cut(s) 2611
XmiI GTMKAC 1 cut(s) 1006
XmnI GAANNNNTTC 4 cut(s) 177, 336, 2202, 2751
XspI CTAG 8 cut(s) 797, 923, 1023, 1199, 1985, 2406, 2561, 2612
ZrmI AGTACT 2 cut(s) 986, 2893
Zsp2I ATGCAT 2 cut(s) 1500, 2467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.