Rh2BG198200

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000307
Physical Location & Seq
Reverse (-)
18189305 .. 18197093
7789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG198200.1

Sequence Viewer

Length: 4878 bp
ATGAGACAGGTGGACAAGTGGAGGAAAGCTTTAGAGGATATAGCCAGTCTCTCCGGATGGCATGTGACACAGGATAGAAGAGAATCAGAAGTTATTCAAGAAGTAGTTGATGCAATTCTAAATGCACTAAATGAAATGTTATCCGTTCCTGAAAGAGAGTTGTGGCTTGATGACAATGCACACAAGTACGAAGGCAGAATGTCATGGCGTTTTGAGGATGCTTGGAACGTGCTGATGGACAATACGCTCTCTCTTAGATTGCTAATACTAATGGATCTTTCCAACTGCCAATATTTAGCCACGACCCCAGACTTCAAACTTTATTTAGATGGCACGGCTATACGGGTGCTGCCTTTATCAATCCAGAATTTGGAAGGCCTTGTTTTGCTAAATCTTAGCGGCTGCAGGAACCTTCCGAGTCTTCCTAGTGTTCTTTGTAGTCATTTGACATCACTGAAATTTCTCTATCTGTCATTGTGCTCAAGCATGGAAAAACTGCCAGAAAACATAGGCTACTTGAAACACTTGGAGGAGCTTGATGCTTGTGATACTGCTATAAGAAAAGTTCCTGATTCCATTTCAGGTCTCAAGAATCTAAAGCTATTGTGTTTCCATGGATGTTCTGGATTCACAGGGTTAGAGTTGCCAAACAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGGGCAATCCCCCATGACATTGGTGATTTATTCTCACTGCAAAGTCTGGATTTAAGTGAGAACAACATTTTCACTGTACCTGAAAGCATCTCTCAGCTTTCGGAGCTCACAGAAATTTCTCTGTATAAGTGTAGCAAACTACGGTCGTTGCCAAAAGATCTTCCATCAAGTCTAAGGAATGTAAATGTACGTGATTGTCCCATGCTGACAAACTCTTTATCTAGTTGGAGGAGATATCCGCCGCAGAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGAGGATGACATCTTTCCTGAACTCCAAGAGTTTCGTTTGGGAACTCTGGAGGAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCAAATCTGAAGAAATTGATCCTTGAAGGTTGTGAAAAATTATCAGAACTTCACCCAACAATTGGGAGTCTCCAACATCTGATTCTTTTGAATTTGAAAGGATGTGTAGATCTAGAGAGCCTTCCCCGTTTCATCAGCTTGAAGTCTCTAAAAATATTTGTTCTTTCAGGATGTTCAAAGCTGAAAGAGTTTCCAGATATTAAGGGAGATATGGATAGTTTGTCTCAACTGCATTTAGATGGGACAGCGTTAAGGCGACTATCAATACCGATGAAGCATTTGAAATGCCCTATTTCGATAAATCTAAGAGGCTGTAGGAACCTTTTCACTGTTCCAGTTCTACGAAGTCTGAAATCTCTCAATCTGTCGGGCTGCTCACGTGTATCCATATTGCCATCTAACCTGGAAAGCATGAAACATTTGGAGGAGCTTGATGCCTCTGAAACTTCTATAACAGCAGTACCCTCATCCATTTCATATTTGGTGAAGCTGGAAGTGCTATCTTTTTGTGGATGTAGAGGTTTGCAGTTGCCTAAACGGTTCTCGGATTTACGATCTTTGAAATCATTAAATCTACGGAGGTGTGGTCTAGCAGAAGAAGCGCTTGATATCCTGTACTGCTTATCCTCTCTGAAGATTTTGGATTTGGGTGAGAACAGTTTTGTGACTATACCTAATGACATCGATCGCTTATCCTCTTTGCGGCAATTGGATTTGAGTGGAAACAATATAGTGAGCATACCGCATGAAATTGGTTGCTTATACTCGTTGCAGCTTTTAGATTTGAGTGAAAACAACTTTGTGAGTATACCTAAGAGCATCTCTAATCTTTCTGAGCTTACAATACTTCGCCTGTTTAGATGTAGTAAGCTACAGTCCTTGCCAAATAATCTTCCCTTCAATCTAAAACATGTTTATGCACAAGAATGTCCTATGTTGAAGTATAATGCCGATACTTTGACAATACGGGCATCCGGAGAAGGGGTCTTTTTCATGCATTGCGGAAAATATGGGCAGGATGATGATCAGCCAAGCCACGTCCCAGTTCCAGCTCCCAAAGACCGCATTGAACTACTGTTTCCTGTTTACATTGCGGACCGAGTTTATGGTAAAAAGCCATTTGCAATTTGTTTTCCACACAGTACAAGAATTCCCAATCTGTGGAGTCATTGGAGGAGTGGCCCTTTTGTAACAATCCCACTATCTGATGGTAACAGTGCGTGTATGGGACTTGCTCTGTTTGTTGTTTTTGAAATCCTTGAAGGCAATTTCAATAAGATATGGGAATTGGAAGAGACTATCTGTGAATTTCACACTGATGTTGGTCCTGAAAATTCCCTTGTTTTTCAAAACTTCATAGACTTCAAGGCTGGGTCATATGCACTTTGTTGCTATGAACCGCGAGGGGGGCAATTTAGTGGGATGTTTGATAAACCAATCTCACAACTTCAAGCTTCAGTTTCAATGAAGAGACCAAATTCAAAAGTGAGAGGTTGTGGGATACATCTAATGTCAGAGGAAGACACTGCAGAGTTTGTAAAAAGTTTAGCAAATCAGACTGCCACTCAACACCTCGATTCCAATTTTGATCAACATTGCGAGTACCTATTAAATGAGGAGACAACAACTGGTGACCTGATGGAAGTGGGATCTACTTCAACTTTCAATGAAGATAGCTGCAGCAAAAGTATCTCTAAATTCAACTTGAGAGGAGAGCTGTCGATACTCTATGAGGGATGCAATGGACGTGAGAATAGTTTCCATTTTTGCTTTCCTGCTCCCGTAATTTCAGCCCCGCCCTGGTTCTTCCATCACCATGCAGGGGATGTAACTTTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCTACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATTTGTGCTCTCATGATCATGAAAGTATAGTAAGGCACGGATCCCTCAAGGTAAACAGTTGTGTTGGGATCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAGCAGTTGAATCAATGTCAGTGTATTAGCGCATTGTTCAGAACCATTGATGCAGAGATGGATGTCCAAGTGTGTGGAAGCCGTTTAGCTTTCGAGCATGATTTGGAAGACTTGATCCATTCATTAACTGCTGCTGTCGGCACGTTGGGGCAACATGTCCTCACTCAACCACGCTCCCCGGCCCAACCTGTTGATCGGCTCAATGCAGAGGAAGCAGAAATGCCCATCAATTGCTGTTCCTGTTTTCAGAGATGCAGAAGTACTGCACTAGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATTATAGATGCTATCTCCAGCAACAGCAAGGTTTTTGGGAGTCTGACGTTAACCAGTTGGTCCACTTCAGGTCAGTAATGTTACTCCACTCCAGAAGCCTTTTGGAAAACAGGGACAGAAACCAAGAAAACATTGCCGAAAAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGCTATAGTTCACCACGGTGGAAGAGATGCCTGAAGTTGTTGCTTAGGCGGTACAAGGTGGCTACTCTCAGTCTCGGTGGACACGAAATTTCAGCTCTCAAGAATTTTGATCCTTCCTCCCCATACAATATTATTTGTTTCTCTGACAACGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTTGGGTGGGAATCAAACTACCTTCAAGATTGCACGAGGATGAGAATTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCATCCAGCTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTTCCATCACTGAAGAGAAACTCAAGTCGTTGCATCTTGGTCGATTCATTTGGGTAACCTACATCCCCAGTATTTTTCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAGAGGCTTGACAGTGGAGAAGTGTGGTTTACGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACTGAATGCTGGACCTCTTACTTCGATAATCTGTCTTTCATCCGTCAACTTGTAGAAGCAGATGAACTTCCAATGCTTGAAGGGCATAACAAGGTATTTGATCCGGATTTAATATACAATGCAATCTGCCCTTCTAATGAAATTCTGGAGTGGTTTGGGCATCGCATTGAGGATTCCTCGGATGACACCTGGGGCTGCGAATTCCAGTTACCACCATCTTTGAGCGACACAAATTGGATAGGATTGTCCATCTGTGTATCATATCGTATTAGAAGAGTGGATTGGTACGAACCTAATTCCCACCCCTTTAATTTAACATTGAAAACCAGAAACAATGCTTTGTCGTCTCTCCATCGGTATCAAATTAGCAATGAAGAGTATCAGTTCATAAACAGTTGTCGTATTATGAATCGTAGATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGTCCTCTTTGCTTCACTCCGGGACACCTGGTGGAGCCCGGCCAAGATCTATCACCGATTTGTATATGCGCAGGAAGAGTTCAAGCAGCTCTGTTTCAATCTCGATCGACCTGGTGCCCCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8

Protein Analysis

1625

Amino Acids

184.97

Weight (kDa)

6.0

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 119 - 230 3.6e-13 Leucine-rich repeat region
LRR_14 PF23598 333 - 447 9.2e-06 Leucine-rich repeat region
LRR_14 PF23598 473 - 583 6.4e-10 Leucine-rich repeat region
LRR_8 PF13855 552 - 611 9.8e-09 Leucine rich repeat
LRR_14 PF23598 574 - 660 1.5e-11 Leucine-rich repeat region
C-JID PF20160 1280 - 1417 1.3e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 4821
AccB1I GGYRCC 1 cut(s) 4865
AccB7I CCANNNNNTGG 3 cut(s) 370, 1163, 2262
AccI GTMKAC 1 cut(s) 1909
AccII CGCG 1 cut(s) 2503
AccIII TCCGGA 3 cut(s) 53, 2075, 4378
AcoI YGGCCR 1 cut(s) 4791
AcuI CTGAAG 7 cut(s) 1130, 1754, 2541, 3541, 3674, 3749, 4101
AcvI CACGTG 2 cut(s) 1481, 3161
AdeI CACNNNGTG 1 cut(s) 4782
AfeI AGCGCT 1 cut(s) 1704
AfiI CCNNNNNNNGG 9 cut(s) 370, 959, 1163, 1803, 2082, 2262, 2507, 2901, 2923
AflIII ACRYGT 5 cut(s) 1480, 2011, 3151, 3160, 3328
AhlI ACTAGT 1 cut(s) 3442
AjiI CACGTC 2 cut(s) 2140, 2849
AjnI CCWGG 5 cut(s) 1503, 2900, 4463, 4778, 4861
AjuI GAANNNNNNNTTGG 4 cut(s) 1167, 1199, 4540, 4572
AleI CACNNNNGTG 1 cut(s) 3712
Alw21I GWGCWC 3 cut(s) 482, 819, 3068
Aor13HI TCCGGA 3 cut(s) 53, 2075, 4378
Aor51HI AGCGCT 1 cut(s) 1704
AoxI GGCC 5 cut(s) 376, 2281, 3354, 4179, 4791
Asp700I GAANNNNTTC 3 cut(s) 93, 1424, 2858
AspA2I CCTAGG 1 cut(s) 688
AspLEI GCGC 3 cut(s) 1705, 3206, 4822
AspS9I GGNCC 6 cut(s) 2197, 2282, 2426, 3355, 3550, 4287
AsuC2I CCSGG 4 cut(s) 3353, 4007, 4772, 4790
AsuHPI GGTGA 8 cut(s) 746, 1145, 1597, 1763, 2744, 2906, 3699, 4796
AvaII GGWCC 4 cut(s) 2197, 2426, 3550, 4287
AvrII CCTAGG 1 cut(s) 688
BaeGI GKGCMC 1 cut(s) 4870
BaeI ACNNNNGTAYC 2 cut(s) 1545, 1578
BamHI GGATCC 1 cut(s) 3098
BanI GGYRCC 1 cut(s) 4865
BanII GRGCYC 2 cut(s) 819, 4790
BarI GAAGNNNNNNTAC 2 cut(s) 3004, 3036
BauI CACGAG 1 cut(s) 3932
BbrPI CACGTG 2 cut(s) 1481, 3161
BbsI GAAGAC 4 cut(s) 413, 2628, 3288, 3882
Bbv12I GWGCWC 3 cut(s) 482, 819, 3068
BceAI ACGGC 2 cut(s) 351, 3240
BcgI CGANNNNNNTGC 2 cut(s) 4091, 4125
BciT130I CCWGG 5 cut(s) 1505, 2902, 4465, 4780, 4863
BciVI GTATCC 2 cut(s) 1495, 2595
BclI TGATCA 4 cut(s) 2125, 2689, 2965, 3073
BcnI CCSGG 4 cut(s) 3353, 4007, 4772, 4790
BcuI ACTAGT 1 cut(s) 3442
BfaI CTAG 7 cut(s) 426, 689, 933, 1214, 1691, 3443, 3894
BfmI CTRYAG 6 cut(s) 403, 1414, 1973, 2628, 2779, 3700
BfoI RGCGCY 1 cut(s) 1706
BfuI GTATCC 2 cut(s) 1495, 2595
BglII AGATCT 4 cut(s) 668, 868, 1210, 4797
BlnI CCTAGG 1 cut(s) 688
BmcAI AGTACT 2 cut(s) 3436, 3447
Bme1390I CCNGG 9 cut(s) 1505, 2902, 3353, 4007, 4465, 4772, 4780, 4790, 4863
Bme18I GGWCC 4 cut(s) 2197, 2426, 3550, 4287
BmgBI CACGTC 2 cut(s) 2140, 2849
BmgT120I GGNCC 6 cut(s) 2197, 2282, 2426, 3355, 3550, 4287
BmiI GGNNCC 6 cut(s) 410, 1101, 1421, 3100, 4787, 4867
BmrFI CCNGG 9 cut(s) 1505, 2902, 3353, 4007, 4465, 4772, 4780, 4790, 4863
BmrI ACTGGG 2 cut(s) 2138, 4131
BmuI ACTGGG 2 cut(s) 2138, 4131
BpiI GAAGAC 4 cut(s) 413, 2628, 3288, 3882
BplI GAGNNNNNCTC 2 cut(s) 4436, 4468
BpmI CTGGAG 4 cut(s) 1061, 3491, 3565, 4442
Bpu10I CCTNAGC 1 cut(s) 3740
BpuEI CTTGAG 7 cut(s) 466, 572, 2827, 3089, 3779, 4048, 4076
BpuMI CCSGG 4 cut(s) 3353, 4007, 4772, 4790
Bsa29I ATCGAT 1 cut(s) 1785
BsaAI YACGTR 3 cut(s) 902, 1481, 3161
BsaBI GATNNNNATC 1 cut(s) 2124
BsaI GGTCTC 2 cut(s) 590, 2566
BsaJI CCNNGG 9 cut(s) 613, 688, 2900, 3351, 3710, 4005, 4035, 4452, 4464
BsaWI WCCGGW 3 cut(s) 53, 2075, 4378
BsaXI ACNNNNNCTCC 4 cut(s) 1672, 1702, 2070, 2100
Bsc4I CCNNNNNNNGG 9 cut(s) 370, 959, 1163, 1803, 2082, 2262, 2507, 2901, 2923
Bse118I RCCGGY 1 cut(s) 4242
Bse1I ACTGG 8 cut(s) 45, 1436, 2144, 2734, 3544, 3854, 4137, 4480
Bse3DI GCAATG 6 cut(s) 2098, 2190, 2695, 2848, 3621, 4651
Bse8I GATNNNNATC 1 cut(s) 2124
BseAI TCCGGA 3 cut(s) 53, 2075, 4378
BseBI CCWGG 5 cut(s) 1505, 2902, 4465, 4780, 4863
BseCI ATCGAT 1 cut(s) 1785
BseDI CCNNGG 9 cut(s) 613, 688, 2900, 3351, 3710, 4005, 4035, 4452, 4464
BseJI GATNNNNATC 1 cut(s) 2124
BseLI CCNNNNNNNGG 9 cut(s) 370, 959, 1163, 1803, 2082, 2262, 2507, 2901, 2923
BseMI GCAATG 6 cut(s) 2098, 2190, 2695, 2848, 3621, 4651
BseMII CTCAG 6 cut(s) 672, 818, 1926, 3144, 3669, 3778
BseNI ACTGG 8 cut(s) 45, 1436, 2144, 2734, 3544, 3854, 4137, 4480
BseRI GAGGAG 7 cut(s) 545, 955, 1007, 1541, 2290, 2732, 2826
BseSI GKGCMC 1 cut(s) 4870
BseYI CCCAGC 1 cut(s) 2471
BsgI GTGCAG 1 cut(s) 3423
Bsh1236I CGCG 1 cut(s) 2503
Bsh1285I CGRYCG 3 cut(s) 857, 1789, 4858
BshFI GGCC 5 cut(s) 378, 2283, 3356, 4181, 4793
BshNI GGYRCC 1 cut(s) 4865
BshVI ATCGAT 1 cut(s) 1785
BsiEI CGRYCG 3 cut(s) 857, 1789, 4858
BsiHKAI GWGCWC 3 cut(s) 482, 819, 3068
BsiSI CCGG 8 cut(s) 54, 2076, 3353, 4007, 4243, 4379, 4771, 4790
BslFI GGGAC 6 cut(s) 894, 1357, 2126, 2343, 3617, 4787
BslI CCNNNNNNNGG 9 cut(s) 370, 959, 1163, 1803, 2082, 2262, 2507, 2901, 2923
BsmBI CGTCTC 2 cut(s) 4238, 4626
BsmFI GGGAC 6 cut(s) 894, 1357, 2126, 2343, 3617, 4787
BsmI GAATGC 1 cut(s) 4286
BsnI GGCC 5 cut(s) 378, 2283, 3356, 4181, 4793
Bso31I GGTCTC 2 cut(s) 590, 2566
Bsp1286I GDGCHC 5 cut(s) 482, 819, 3068, 4790, 4870
Bsp13I TCCGGA 3 cut(s) 53, 2075, 4378
Bsp1407I TGTACA 2 cut(s) 3011, 3664
Bsp19I CCATGG 1 cut(s) 613
BspANI GGCC 5 cut(s) 378, 2283, 3356, 4181, 4793
BspCNI CTCAG 6 cut(s) 671, 817, 1927, 3143, 3670, 3777
BspDI ATCGAT 1 cut(s) 1785
BspEI TCCGGA 3 cut(s) 53, 2075, 4378
BspFNI CGCG 1 cut(s) 2503
BspHI TCATGA 3 cut(s) 3070, 3076, 3982
BspLI GGNNCC 6 cut(s) 410, 1101, 1421, 3100, 4787, 4867
BspMAI CTGCAG 3 cut(s) 407, 2632, 2783
BspT107I GGYRCC 1 cut(s) 4865
BspTNI GGTCTC 2 cut(s) 590, 2566
BsrDI GCAATG 6 cut(s) 2098, 2190, 2695, 2848, 3621, 4651
BsrFI RCCGGY 1 cut(s) 4242
BsrGI TGTACA 2 cut(s) 3011, 3664
BsrI ACTGG 8 cut(s) 45, 1436, 2144, 2734, 3544, 3854, 4137, 4480
BssAI RCCGGY 1 cut(s) 4242
BssECI CCNNGG 9 cut(s) 613, 688, 2900, 3351, 3710, 4005, 4035, 4452, 4464
BssNAI GTATAC 1 cut(s) 1910
BssSI CACGAG 1 cut(s) 3932
BssT1I CCWWGG 3 cut(s) 613, 688, 4035
Bst1107I GTATAC 1 cut(s) 1910
Bst2BI CACGAG 1 cut(s) 3932
Bst2UI CCWGG 5 cut(s) 1505, 2902, 4465, 4780, 4863
Bst6I CTCTTC 9 cut(s) 73, 2388, 2564, 3713, 4077, 4245, 4543, 4644, 4821
BstAUI TGTACA 2 cut(s) 3011, 3664
BstBAI YACGTR 3 cut(s) 902, 1481, 3161
BstDSI CCRYGG 2 cut(s) 613, 3710
BstEII GGTNACC 2 cut(s) 2732, 4123
BstFNI CGCG 1 cut(s) 2503
BstH2I RGCGCY 1 cut(s) 1706
BstHHI GCGC 3 cut(s) 1705, 3206, 4822
BstMCI CGRYCG 3 cut(s) 857, 1789, 4858
BstMWI GCNNNNNNNGC 7 cut(s) 713, 814, 1597, 1700, 2509, 3386, 4357
BstNI CCWGG 5 cut(s) 1505, 2902, 4465, 4780, 4863
BstNSI RCATGY 4 cut(s) 65, 2015, 3155, 3332
BstPI GGTNACC 2 cut(s) 2732, 4123
BstSCI CCNGG 9 cut(s) 1503, 2900, 3351, 4005, 4463, 4770, 4778, 4788, 4861
BstSFI CTRYAG 6 cut(s) 403, 1414, 1973, 2628, 2779, 3700
BstSLI GKGCMC 1 cut(s) 4870
BstUI CGCG 1 cut(s) 2503
BstV2I GAAGAC 4 cut(s) 413, 2628, 3288, 3882
BstX2I RGATCY 9 cut(s) 274, 668, 868, 1080, 1210, 2750, 3098, 3126, 4797
BstXI CCANNNNNNTGG 3 cut(s) 731, 3248, 4722
BstYI RGATCY 9 cut(s) 274, 668, 868, 1080, 1210, 2750, 3098, 3126, 4797
BstZ17I GTATAC 1 cut(s) 1910
Bsu15I ATCGAT 1 cut(s) 1785
BsuI GTATCC 2 cut(s) 1495, 2595
BsuRI GGCC 5 cut(s) 378, 2283, 3356, 4181, 4793
BsuTUI ATCGAT 1 cut(s) 1785
BtgI CCRYGG 2 cut(s) 613, 3710
BtgZI GCGATG 1 cut(s) 4421
BtrI CACGTC 2 cut(s) 2140, 2849
BtsI GCAGTG 2 cut(s) 746, 2625
CciI TCATGA 3 cut(s) 3070, 3076, 3982
CfoI GCGC 3 cut(s) 1705, 3206, 4822
Cfr10I RCCGGY 1 cut(s) 4242
Cfr13I GGNCC 6 cut(s) 2197, 2282, 2426, 3355, 3550, 4287
ClaI ATCGAT 1 cut(s) 1785
CpoI CGGWCCG 1 cut(s) 2197
CseI GACGC 1 cut(s) 4728
CsiI ACCWGGT 2 cut(s) 4778, 4861
CspCI CAANNNNNGTGG 2 cut(s) 4476, 4511
CspI CGGWCCG 1 cut(s) 2197
DraIII CACNNNGTG 1 cut(s) 4782
EaeI YGGCCR 1 cut(s) 4791
Eam1104I CTCTTC 9 cut(s) 73, 2388, 2564, 3713, 4077, 4245, 4543, 4644, 4821
EarI CTCTTC 9 cut(s) 73, 2388, 2564, 3713, 4077, 4245, 4543, 4644, 4821
EciI GGCGGA 1 cut(s) 939
Ecl136II GAGCTC 1 cut(s) 817
Eco130I CCWWGG 3 cut(s) 613, 688, 4035
Eco147I AGGCCT 1 cut(s) 378
Eco24I GRGCYC 2 cut(s) 819, 4790
Eco31I GGTCTC 2 cut(s) 590, 2566
Eco32I GATATC 2 cut(s) 947, 1711
Eco47I GGWCC 4 cut(s) 2197, 2426, 3550, 4287
Eco47III AGCGCT 1 cut(s) 1704
Eco53kI GAGCTC 1 cut(s) 817
Eco57I CTGAAG 7 cut(s) 1130, 1754, 2541, 3541, 3674, 3749, 4101
Eco72I CACGTG 2 cut(s) 1481, 3161
Eco91I GGTNACC 2 cut(s) 2732, 4123
EcoICRI GAGCTC 1 cut(s) 817
EcoO65I GGTNACC 2 cut(s) 2732, 4123
EcoRI GAATTC 2 cut(s) 2250, 4475
EcoRII CCWGG 5 cut(s) 1503, 2900, 4463, 4778, 4861
EcoRV GATATC 2 cut(s) 947, 1711
EcoT14I CCWWGG 3 cut(s) 613, 688, 4035
EcoT22I ATGCAT 1 cut(s) 2100
EcoT38I GRGCYC 2 cut(s) 819, 4790
ErhI CCWWGG 3 cut(s) 613, 688, 4035
Esp3I CGTCTC 2 cut(s) 4238, 4626
FalI AAGNNNNNCTT 2 cut(s) 1689, 1721
FaqI GGGAC 6 cut(s) 894, 1357, 2126, 2343, 3617, 4787
FauI CCCGC 1 cut(s) 2904
FauNDI CATATG 1 cut(s) 2479
FbaI TGATCA 4 cut(s) 2125, 2689, 2965, 3073
FblI GTMKAC 1 cut(s) 1909
FriOI GRGCYC 2 cut(s) 819, 4790
FspBI CTAG 7 cut(s) 426, 689, 933, 1214, 1691, 3443, 3894
FspI TGCGCA 1 cut(s) 4821
GlaI GCGC 3 cut(s) 1704, 3205, 4821
GsaI CCCAGC 1 cut(s) 2475
GsuI CTGGAG 4 cut(s) 1061, 3491, 3565, 4442
HaeII RGCGCY 1 cut(s) 1706
HaeIII GGCC 5 cut(s) 378, 2283, 3356, 4181, 4793
HapII CCGG 8 cut(s) 54, 2076, 3353, 4007, 4243, 4379, 4771, 4790
HgaI GACGC 1 cut(s) 4728
HhaI GCGC 3 cut(s) 1705, 3206, 4822
Hin6I GCGC 3 cut(s) 1703, 3204, 4820
HinP1I GCGC 3 cut(s) 1703, 3204, 4820
HincII GTYRAC 2 cut(s) 3541, 4322
HindII GTYRAC 2 cut(s) 3541, 4322
HindIII AAGCTT 2 cut(s) 27, 2553
HpaI GTTAAC 1 cut(s) 3541
HpaII CCGG 8 cut(s) 54, 2076, 3353, 4007, 4243, 4379, 4771, 4790
HphI GGTGA 8 cut(s) 746, 1145, 1597, 1763, 2744, 2906, 3699, 4796
Hpy99I CGWCG 1 cut(s) 4722
HpyCH4IV ACGT 9 cut(s) 228, 901, 1480, 2139, 2848, 3160, 3317, 3537, 4231
HpyF10VI GCNNNNNNNGC 7 cut(s) 713, 814, 1597, 1700, 2509, 3386, 4357
HpySE526I ACGT 9 cut(s) 228, 901, 1480, 2139, 2848, 3160, 3317, 3537, 4231
HspAI GCGC 3 cut(s) 1703, 3204, 4820
Kpn2I TCCGGA 3 cut(s) 53, 2075, 4378
Ksp22I TGATCA 4 cut(s) 2125, 2689, 2965, 3073
KspAI GTTAAC 1 cut(s) 3541
LmnI GCTCC 8 cut(s) 532, 814, 1528, 2158, 2884, 3353, 3466, 4785
MabI ACCWGGT 2 cut(s) 4778, 4861
MaeI CTAG 7 cut(s) 426, 689, 933, 1214, 1691, 3443, 3894
MaeII ACGT 9 cut(s) 228, 901, 1480, 2139, 2848, 3160, 3317, 3537, 4231
MfeI CAATTG 4 cut(s) 1161, 1808, 3403, 3474
MflI RGATCY 9 cut(s) 274, 668, 868, 1080, 1210, 2750, 3098, 3126, 4797
MhlI GDGCHC 5 cut(s) 482, 819, 3068, 4790, 4870
MlyI GAGTC 6 cut(s) 427, 1177, 2275, 3539, 3676, 4757
MmeI TCCRAC 3 cut(s) 306, 917, 1198
Mph1103I ATGCAT 1 cut(s) 2100
MroI TCCGGA 3 cut(s) 53, 2075, 4378
MroXI GAANNNNTTC 3 cut(s) 93, 1424, 2858
MslI CAYNNNNRTG 8 cut(s) 1338, 2016, 2026, 2418, 2916, 3075, 3712, 3936
MspI CCGG 8 cut(s) 54, 2076, 3353, 4007, 4243, 4379, 4771, 4790
MspR9I CCNGG 9 cut(s) 1505, 2902, 3353, 4007, 4465, 4772, 4780, 4790, 4863
MunI CAATTG 4 cut(s) 1161, 1808, 3403, 3474
Mva1269I GAATGC 1 cut(s) 4286
MvaI CCWGG 5 cut(s) 1505, 2902, 4465, 4780, 4863
MvnI CGCG 1 cut(s) 2503
MwoI GCNNNNNNNGC 7 cut(s) 713, 814, 1597, 1700, 2509, 3386, 4357
NciI CCSGG 4 cut(s) 3353, 4007, 4772, 4790
NcoI CCATGG 1 cut(s) 613
NdeI CATATG 1 cut(s) 2479
NlaIV GGNNCC 6 cut(s) 410, 1101, 1421, 3100, 4787, 4867
NmuCI GTSAC 4 cut(s) 64, 1765, 2732, 4022
NsbI TGCGCA 1 cut(s) 4821
NsiI ATGCAT 1 cut(s) 2100
NspI RCATGY 4 cut(s) 65, 2015, 3155, 3332
OliI CACNNNNGTG 1 cut(s) 3712
PagI TCATGA 3 cut(s) 3070, 3076, 3982
PceI AGGCCT 1 cut(s) 378
PciI ACATGT 3 cut(s) 2011, 3151, 3328
PcsI WCGNNNNNNNCGW 1 cut(s) 3777
PctI GAATGC 1 cut(s) 4286
PdmI GAANNNNTTC 3 cut(s) 93, 1424, 2858
PflMI CCANNNNNTGG 3 cut(s) 370, 1163, 2262
PfoI TCCNGGA 1 cut(s) 4770
Ple19I CGATCG 2 cut(s) 1789, 4858
PleI GAGTC 6 cut(s) 426, 1176, 2274, 3538, 3676, 4756
PmaCI CACGTG 2 cut(s) 1481, 3161
PmlI CACGTG 2 cut(s) 1481, 3161
PpsI GAGTC 6 cut(s) 426, 1176, 2274, 3538, 3676, 4756
Ppu21I YACGTR 3 cut(s) 902, 1481, 3161
PscI ACATGT 3 cut(s) 2011, 3151, 3328
Psp124BI GAGCTC 1 cut(s) 819
Psp6I CCWGG 5 cut(s) 1503, 2900, 4463, 4778, 4861
PspCI CACGTG 2 cut(s) 1481, 3161
PspEI GGTNACC 2 cut(s) 2732, 4123
PspFI CCCAGC 1 cut(s) 2471
PspGI CCWGG 5 cut(s) 1503, 2900, 4463, 4778, 4861
PspN4I GGNNCC 6 cut(s) 410, 1101, 1421, 3100, 4787, 4867
PspPI GGNCC 6 cut(s) 2197, 2282, 2426, 3355, 3550, 4287
PsrI GAACNNNNNNTAC 2 cut(s) 3147, 3179
PstI CTGCAG 3 cut(s) 407, 2632, 2783
PsuI RGATCY 9 cut(s) 274, 668, 868, 1080, 1210, 2750, 3098, 3126, 4797
PvuI CGATCG 2 cut(s) 1789, 4858
RseI CAYNNNNRTG 8 cut(s) 1338, 2016, 2026, 2418, 2916, 3075, 3712, 3936
Rsr2I CGGWCCG 1 cut(s) 2197
RsrII CGGWCCG 1 cut(s) 2197
SacI GAGCTC 1 cut(s) 819
Sau96I GGNCC 6 cut(s) 2197, 2282, 2426, 3355, 3550, 4287
ScaI AGTACT 2 cut(s) 3436, 3447
SchI GAGTC 6 cut(s) 427, 1177, 2275, 3539, 3676, 4757
ScrFI CCNGG 9 cut(s) 1505, 2902, 3353, 4007, 4465, 4772, 4780, 4790, 4863
SduI GDGCHC 5 cut(s) 482, 819, 3068, 4790, 4870
SexAI ACCWGGT 2 cut(s) 4778, 4861
SfcI CTRYAG 6 cut(s) 403, 1414, 1973, 2628, 2779, 3700
SinI GGWCC 4 cut(s) 2197, 2426, 3550, 4287
SmiMI CAYNNNNRTG 8 cut(s) 1338, 2016, 2026, 2418, 2916, 3075, 3712, 3936
SmlI CTYRAG 7 cut(s) 481, 587, 2806, 3104, 3794, 4027, 4091
SmoI CTYRAG 7 cut(s) 481, 587, 2806, 3104, 3794, 4027, 4091
SpeI ACTAGT 1 cut(s) 3442
SseBI AGGCCT 1 cut(s) 378
SspI AATATT 3 cut(s) 293, 1257, 3826
SspMI CTAG 7 cut(s) 426, 689, 933, 1214, 1691, 3443, 3894
SstI GAGCTC 1 cut(s) 819
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 9 cut(s) 1503, 2900, 3351, 4005, 4463, 4770, 4778, 4788, 4861
StyI CCWWGG 3 cut(s) 613, 688, 4035
TaiI ACGT 9 cut(s) 231, 904, 1483, 2142, 2851, 3163, 3320, 3540, 4234
TaqII GACCGA 1 cut(s) 2214
TatI WGTACW 6 cut(s) 1716, 2243, 3011, 3434, 3445, 3664
TauI GCSGC 4 cut(s) 402, 955, 1807, 3484
TseFI GTSAC 4 cut(s) 64, 1765, 2732, 4022
Tsp45I GTSAC 4 cut(s) 64, 1765, 2732, 4022
TspGWI ACGGA 5 cut(s) 133, 1693, 3111, 4166, 4307
Van91I CCANNNNNTGG 3 cut(s) 370, 1163, 2262
VpaK11BI GGWCC 4 cut(s) 2197, 2426, 3550, 4287
XbaI TCTAGA 1 cut(s) 1213
XceI RCATGY 4 cut(s) 65, 2015, 3155, 3332
XcmI CCANNNNNNNNNTGG 3 cut(s) 620, 1579, 3589
XmaJI CCTAGG 1 cut(s) 688
XmiI GTMKAC 1 cut(s) 1909
XmnI GAANNNNTTC 3 cut(s) 93, 1424, 2858
XspI CTAG 7 cut(s) 426, 689, 933, 1214, 1691, 3443, 3894
ZrmI AGTACT 2 cut(s) 3436, 3447
Zsp2I ATGCAT 1 cut(s) 2100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.