Rh2AG187500

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000307
Physical Location & Seq
Reverse (-)
18412205 .. 18416052
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG187500.1

Sequence Viewer

Length: 3024 bp
ATGGAACATTTGGAGAAGCTTGATGCCTCTGAAACTGCTATAACTGAAGTACCATATTCCATTTTATATTTGGAGAAGCTCAAAGTGTTATCTTTCTGTGGATGTTTTGGTTTGCAGTTACCTAACTGGTTCTCGAATTTAAGATCTTTGAGATCATTAAATCTACGTAGGTGTGGTCTACCAGAAAATGTTCATGGTAGGCTCTTCTGGTTGACCTCACTGCAGAGTTTGGATTTGGGTGAAAACAATTTTGATCGAATCTATGGTAAAAAACCATTTGAATTTCGTTTTCCACATAGTGCAAGGATTCCCCAATTTTGGAGCCATTGGAGGAGTGGCCCTTCTGTGGCAGTCCGGCTATCTGATAGTAACGGTTCGTGGATTGGACTTGCTCTATTTGTTGTTTTCGAAATCCTTGAGATAGAAAAATTCGACAACAGATGGGAATCAGAGGAGACTATCTGTGAATTTCACACCAGTTTTGGGCGTGAAAATTCACTAGTTTTTCAGAACTTCCTAGACTTCAGGACTGGTTCATATGGACTTTGTTGCTACGAACCACGAGGTGGGCAATTTGGTGGGTTGTTTGATGAACCACTACTTCAAGCTTCAGTTTCAACAAAGAGACCAGATTTAAAAGTGAGAGGTTGTGGGTTGCATCTGATATCAGAGCACGATGCTGCAGCATTTGTTCAAAATTTAATAAACCAGACTGCTATTCAACACCTGGATTTCAATTTTGATCGACATTGTGAGGAAATATTAGAAAAGGCAACGATTTGTGACCTGATAGAGCTAGACCCTGATGATCTATGGGAACTAGGATCTTCTTCAACTGTGAATGAAGATGACTGCCGCATGAGTAGCTGTAAAATCCAACTCAGAGCACAGCTGTCAATCCTCTATGAGGGACGCAGTGGATGTGAGAAGAGTTTCCATGTTTGTCTTCCTGATTCACCAAATATAATGTCAATTCCGACCTGGTTCATCAATCGCCAAGCAGGTGATGTAACACTATGTTACATTAGTGAAAACTTGTTTGCTGATCAGAGATGGGTGGGATTAGAACTATATGTTGTTTTTGCCCGGCGTACACCTGCTCCAAGTGATGGCCATATTAATAGTTTCTTCTTTCATGTTGAGTTGTCTGGTCATGATAATGGAAGTCTAATAATGCATGGATCCCTCAAGAGAGAGAGTTGTCTTGGAGAGTCAAATCAACTTCTTGTATTACATTTACCACGCGTTTATTTTCAAAAACAGTTAAATCAATGTCAGGTTATTAGCGCATTGTTTACAACCAGTACCCCAGAATTGGAGGTTATAGTGTGTGGGAGCCGTTTAGTTTATCAGCAAGATTTGAAAGACTTGATCCATTCATTGACTGCGGCTGCAAGCACTTTTGGGGAACATGTCCTCACTCAACTTTGTTCCCAGGCCTGCACCCAAACTGATGATCAGATCATTGCAGTGAAAGGAGAAATGGCGATCAATTGCGATTCCTCATTTCAAAGCAGAAGAAGTACAGCACAATTACCAGAACAAGTTCTTACTTCAATAATGCGGCTGAAAAGTTATAGATGCTATCTTCGGCAAAAGCAAGTTTTTGTAGCGTCTGAACCTACCCCATTAGTTCACTCCGGATCAGTGATACCAGTCCATGCCAAAGGCTGTCTTTTGGAATACAGGGAAAGCAACTCAGAAAATGTAGCAGAAGAAAACACAAAGTCGTCATTGGCTTATTGTCATCATTTACGTATAATGGCTGAGACTCAACTTTACGGAAGCTATAGTTCATTTCAGTTGAAAACATCCCTCCAGTTGTTACTCCAACACTCCAAGGTGGCCACTCTCAGTCTTGGTGGACATACAATTTCTTCTCTCAAGAATTTTGATCCTTCCTCCGCATGCAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCTTATCAACTGTCTTCTAGGTCCAGGGTGGGAATCAAACTACCTCCAAAATTGCAAGAGGATGGGAATTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCATCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAGCTATTGCTTGAATCCTATCCCTATGTGTTCAATCACTGAAGAGAAACTCAAGTCGTTGCATGTTGATAGATTCATTTGGCTAAGCTACATCCCCCATGTTTTGCTAACGGAGTTCAATGTGGTTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAAAGGCTTGACAGTGGAGAAGTGTGGTATGCGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAGCACGATAACTGAGTGCTGGACCTCTTTCTTTGATAATCTGTCTTTCATCCGTAGACTTGTAGAAGCAGATGATCAAAATGTTCAACCATGGACAAGGCATGAACTTCCAATGCTTGAAGGGCATATCAAGGTCTTTGATCCGGATATACTTTATAATGCAATTCCCACTTCTACTGAAATTCCGAAGTGGTTTGGGCATCCCATTAACGCCAAGGACGATTACGCCATGAACTGGTATGAATACCAGTTACCACCATCTTTGAGTGACAAAAACTGGATAGGATTGGCTATCTGTGCATCACATGGTATTAGCAGAGGGTATTTGGTGGAACGTGATTCCTACCCCTTTATTTTAACATTGAAAACTGCAGACAATGGCTTGTCATCTCTCCATCGGTATCAGATGACCAATGAAGAATCTGAGTTCCTAAAGCGTTGTTGTAATGTGCATGGTGAATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGACGAGTCTGTCCTCATTGTTTCATCTAGTGTGAACAGCTGGAGGCCGCGCACGGTATATCTCCGTTTTGTGTATGCGCATGAGGTGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGCCTGCCCAACAGTAAAAAGATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1007

Amino Acids

115.18

Weight (kDa)

6.0

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 2 - 81 2.8e-07 Leucine-rich repeat region
C-JID PF20160 643 - 780 9e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2511
AarI CACCTGC 2 cut(s) 992, 1105
AasI GACNNNNNNGTC 1 cut(s) 2873
Acc16I TGCGCA 1 cut(s) 2943
Acc36I ACCTGC 2 cut(s) 992, 1105
AccB7I CCANNNNNTGG 3 cut(s) 566, 1109, 2589
AccI GTMKAC 2 cut(s) 178, 2410
AccII CGCG 2 cut(s) 1245, 2914
AccIII TCCGGA 2 cut(s) 1640, 2497
AciI CCGC 5 cut(s) 856, 1388, 1564, 1905, 2912
AclWI GGATC 7 cut(s) 832, 1176, 1189, 1366, 1651, 1889, 2489
AcoI YGGCCR 2 cut(s) 1111, 1845
AcuI CTGAAG 4 cut(s) 66, 508, 594, 2190
AdeI CACNNNGTG 2 cut(s) 299, 566
AfaI GTAC 5 cut(s) 51, 1093, 1306, 1525, 2330
AfiI CCNNNNNNNGG 8 cut(s) 318, 346, 483, 566, 907, 1109, 1315, 2589
AflIII ACRYGT 2 cut(s) 1243, 1411
AhlI ACTAGT 1 cut(s) 499
AjnI CCWGG 4 cut(s) 726, 980, 1434, 1988
Alw21I GWGCWC 3 cut(s) 675, 889, 2360
Alw26I GTCTC 4 cut(s) 449, 619, 1763, 2327
AlwI GGATC 7 cut(s) 832, 1176, 1189, 1366, 1651, 1889, 2489
Aor13HI TCCGGA 2 cut(s) 1640, 2497
AoxI GGCC 6 cut(s) 337, 1111, 1437, 1845, 2268, 2909
ApeKI GCWGC 4 cut(s) 680, 683, 1391, 2965
ArsI GACNNNNNNTTYG 2 cut(s) 953, 985
AseI ATTAAT 1 cut(s) 1119
Asp700I GAANNNNTTC 3 cut(s) 189, 932, 1545
AspLEI GCGC 3 cut(s) 1289, 2916, 2944
AspS9I GGNCC 3 cut(s) 338, 1986, 2376
AsuC2I CCSGG 2 cut(s) 1087, 2096
AsuHPI GGTGA 4 cut(s) 251, 948, 1016, 2822
AsuII TTCGAA 1 cut(s) 408
AvaII GGWCC 2 cut(s) 1986, 2376
BaeI ACNNNNGTAYC 2 cut(s) 33, 66
BalI TGGCCA 2 cut(s) 1113, 1847
BamHI GGATCC 1 cut(s) 1181
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 2 cut(s) 938, 1971
Bbv12I GWGCWC 3 cut(s) 675, 889, 2360
BbvI GCAGC 4 cut(s) 667, 695, 1378, 2977
BccI CCATC 6 cut(s) 435, 1047, 1103, 2021, 2620, 2757
BceAI ACGGC 1 cut(s) 1323
BciT130I CCWGG 4 cut(s) 728, 982, 1436, 1990
BclI TGATCA 3 cut(s) 1045, 1456, 2428
BcnI CCSGG 2 cut(s) 1087, 2096
BcoDI GTCTC 4 cut(s) 449, 619, 1763, 2327
BcuI ACTAGT 1 cut(s) 499
BfaI CTAG 6 cut(s) 500, 518, 797, 821, 1983, 2892
BfmI CTRYAG 4 cut(s) 221, 681, 1789, 2724
BfuAI ACCTGC 2 cut(s) 992, 1105
BglII AGATCT 1 cut(s) 143
BisI GCNGC 8 cut(s) 681, 684, 856, 1389, 1392, 1565, 2912, 2966
BlpI GCTNAGC 1 cut(s) 2213
BlsI GCNGC 8 cut(s) 682, 685, 857, 1390, 1393, 1566, 2913, 2967
Bme1390I CCNGG 6 cut(s) 728, 982, 1087, 1436, 1990, 2096
Bme18I GGWCC 2 cut(s) 1986, 2376
BmgT120I GGNCC 3 cut(s) 338, 1986, 2376
BmiI GGNNCC 3 cut(s) 323, 1183, 1337
BmrFI CCNGG 6 cut(s) 728, 982, 1087, 1436, 1990, 2096
BmsI GCATC 7 cut(s) 13, 667, 667, 1571, 2089, 2563, 2663
BpiI GAAGAC 2 cut(s) 938, 1971
BpmI CTGGAG 2 cut(s) 1802, 2926
Bpu1102I GCTNAGC 1 cut(s) 2213
Bpu14I TTCGAA 1 cut(s) 408
BpuEI CTTGAG 5 cut(s) 437, 1172, 1868, 2137, 2165
BpuMI CCSGG 2 cut(s) 1087, 2096
BsaAI YACGTR 2 cut(s) 167, 1757
BsaBI GATNNNNATC 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 7 cut(s) 1434, 1839, 1989, 2094, 2124, 2444, 2568
BsaWI WCCGGW 2 cut(s) 1640, 2497
BsaXI ACNNNNNCTCC 2 cut(s) 1084, 1114
Bsc4I CCNNNNNNNGG 8 cut(s) 318, 346, 483, 566, 907, 1109, 1315, 2589
Bse118I RCCGGY 1 cut(s) 2331
Bse3DI GCAATG 1 cut(s) 1464
Bse8I GATNNNNATC 1 cut(s) 445
BseAI TCCGGA 2 cut(s) 1640, 2497
BseBI CCWGG 4 cut(s) 728, 982, 1436, 1990
BseDI CCNNGG 7 cut(s) 1434, 1839, 1989, 2094, 2124, 2444, 2568
BseGI GGATG 8 cut(s) 107, 926, 1811, 2032, 2080, 2220, 2403, 2554
BseJI GATNNNNATC 1 cut(s) 445
BseLI CCNNNNNNNGG 8 cut(s) 318, 346, 483, 566, 907, 1109, 1315, 2589
BseMI GCAATG 1 cut(s) 1464
BseMII CTCAG 6 cut(s) 895, 1713, 1758, 1867, 2358, 2769
BseRI GAGGAG 2 cut(s) 346, 467
BseXI GCAGC 4 cut(s) 667, 695, 1378, 2977
BsgI GTGCAG 1 cut(s) 1426
Bsh1236I CGCG 2 cut(s) 1245, 2914
BshFI GGCC 6 cut(s) 339, 1113, 1439, 1847, 2270, 2911
BsiHKAI GWGCWC 3 cut(s) 675, 889, 2360
BsiSI CCGG 6 cut(s) 355, 1087, 1641, 2096, 2332, 2498
BslFI GGGAC 1 cut(s) 924
BslI CCNNNNNNNGG 8 cut(s) 318, 346, 483, 566, 907, 1109, 1315, 2589
BsmAI GTCTC 4 cut(s) 449, 619, 1763, 2327
BsmBI CGTCTC 1 cut(s) 2327
BsmFI GGGAC 1 cut(s) 924
BsnI GGCC 6 cut(s) 339, 1113, 1439, 1847, 2270, 2911
Bso31I GGTCTC 1 cut(s) 619
Bsp119I TTCGAA 1 cut(s) 408
Bsp1286I GDGCHC 3 cut(s) 675, 889, 2360
Bsp13I TCCGGA 2 cut(s) 1640, 2497
Bsp1720I GCTNAGC 1 cut(s) 2213
Bsp19I CCATGG 1 cut(s) 2444
BspACI CCGC 5 cut(s) 856, 1388, 1564, 1905, 2912
BspANI GGCC 6 cut(s) 339, 1113, 1439, 1847, 2270, 2911
BspCNI CTCAG 6 cut(s) 894, 1712, 1759, 1866, 2359, 2770
BspEI TCCGGA 2 cut(s) 1640, 2497
BspFNI CGCG 2 cut(s) 1245, 2914
BspHI TCATGA 2 cut(s) 1153, 2071
BspLI GGNNCC 3 cut(s) 323, 1183, 1337
BspMAI CTGCAG 3 cut(s) 225, 685, 2728
BspMI ACCTGC 2 cut(s) 992, 1105
BspPI GGATC 7 cut(s) 832, 1176, 1189, 1366, 1651, 1889, 2489
BspQI GCTCTTC 1 cut(s) 209
BspT104I TTCGAA 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 619
BsrDI GCAATG 1 cut(s) 1464
BsrFI RCCGGY 1 cut(s) 2331
BssAI RCCGGY 1 cut(s) 2331
BssECI CCNNGG 7 cut(s) 1434, 1839, 1989, 2094, 2124, 2444, 2568
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 4 cut(s) 1839, 2124, 2444, 2568
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 4 cut(s) 728, 982, 1436, 1990
Bst4CI ACNGT 7 cut(s) 374, 838, 1263, 1977, 2302, 2920, 3007
Bst6I CTCTTC 5 cut(s) 209, 923, 2166, 2334, 2949
BstBAI YACGTR 2 cut(s) 167, 1757
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 4 cut(s) 1396, 1441, 1909, 2998
BstDEI CTNAG 7 cut(s) 881, 1699, 1767, 1853, 2213, 2367, 2778
BstDSI CCRYGG 1 cut(s) 2444
BstENI CCTNNNNNAGG 1 cut(s) 905
BstF5I GGATG 8 cut(s) 107, 926, 1811, 2032, 2080, 2220, 2403, 2554
BstFNI CGCG 2 cut(s) 1245, 2914
BstHHI GCGC 3 cut(s) 1289, 2916, 2944
BstMAI GTCTC 4 cut(s) 449, 619, 1763, 2327
BstMWI GCNNNNNNNGC 3 cut(s) 864, 2476, 2651
BstNI CCWGG 4 cut(s) 728, 982, 1436, 1990
BstNSI RCATGY 3 cut(s) 1415, 1911, 2195
BstSCI CCNGG 6 cut(s) 726, 980, 1085, 1434, 1988, 2094
BstSFI CTRYAG 4 cut(s) 221, 681, 1789, 2724
BstSNI TACGTA 2 cut(s) 167, 1757
BstUI CGCG 2 cut(s) 1245, 2914
BstV1I GCAGC 4 cut(s) 667, 695, 1378, 2977
BstV2I GAAGAC 2 cut(s) 938, 1971
BstX2I RGATCY 3 cut(s) 143, 824, 1181
BstXI CCANNNNNNTGG 1 cut(s) 2844
BstYI RGATCY 3 cut(s) 143, 824, 1181
BsuRI GGCC 6 cut(s) 339, 1113, 1439, 1847, 2270, 2911
BtgI CCRYGG 1 cut(s) 2444
BtsCI GGATG 8 cut(s) 107, 926, 1811, 2032, 2080, 2220, 2403, 2554
BtsI GCAGTG 3 cut(s) 218, 922, 1476
BtsIMutI CAGTG 7 cut(s) 218, 922, 1476, 1653, 1950, 2166, 2307
BveI ACCTGC 2 cut(s) 992, 1105
Cac8I GCNNGC 4 cut(s) 1396, 1441, 1909, 2998
CciI TCATGA 2 cut(s) 1153, 2071
CfoI GCGC 3 cut(s) 1289, 2916, 2944
Cfr10I RCCGGY 1 cut(s) 2331
Cfr13I GGNCC 3 cut(s) 338, 1986, 2376
CseI GACGC 4 cut(s) 921, 1602, 2309, 2850
CsiI ACCWGGT 1 cut(s) 980
Csp6I GTAC 5 cut(s) 50, 1092, 1305, 1524, 2329
CspCI CAANNNNNGTGG 2 cut(s) 2598, 2633
CviQI GTAC 5 cut(s) 50, 1092, 1305, 1524, 2329
DdeI CTNAG 7 cut(s) 881, 1699, 1767, 1853, 2213, 2367, 2778
DraI TTTAAA 1 cut(s) 636
DraIII CACNNNGTG 2 cut(s) 299, 566
DrdI GACNNNNNNGTC 1 cut(s) 2873
DseDI GACNNNNNNGTC 1 cut(s) 2873
EaeI YGGCCR 2 cut(s) 1111, 1845
Eam1104I CTCTTC 5 cut(s) 209, 923, 2166, 2334, 2949
EarI CTCTTC 5 cut(s) 209, 923, 2166, 2334, 2949
Eco105I TACGTA 2 cut(s) 167, 1757
Eco130I CCWWGG 4 cut(s) 1839, 2124, 2444, 2568
Eco147I AGGCCT 1 cut(s) 1439
Eco31I GGTCTC 1 cut(s) 619
Eco32I GATATC 1 cut(s) 666
Eco47I GGWCC 2 cut(s) 1986, 2376
Eco57I CTGAAG 4 cut(s) 66, 508, 594, 2190
EcoNI CCTNNNNNAGG 1 cut(s) 905
EcoRI GAATTC 1 cut(s) 2813
EcoRII CCWGG 4 cut(s) 726, 980, 1434, 1988
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 4 cut(s) 1839, 2124, 2444, 2568
EcoT22I ATGCAT 1 cut(s) 1179
ErhI CCWWGG 4 cut(s) 1839, 2124, 2444, 2568
Esp3I CGTCTC 1 cut(s) 2327
FalI AAGNNNNNCTT 2 cut(s) 1659, 1691
FaqI GGGAC 1 cut(s) 924
FauNDI CATATG 1 cut(s) 538
FbaI TGATCA 3 cut(s) 1045, 1456, 2428
FblI GTMKAC 2 cut(s) 178, 2410
Fnu4HI GCNGC 8 cut(s) 681, 684, 856, 1389, 1392, 1565, 2912, 2966
FokI GGATG 8 cut(s) 114, 933, 1798, 2039, 2067, 2207, 2390, 2541
Fsp4HI GCNGC 8 cut(s) 681, 684, 856, 1389, 1392, 1565, 2912, 2966
FspAI RTGCGCAY 1 cut(s) 2943
FspBI CTAG 6 cut(s) 500, 518, 797, 821, 1983, 2892
FspI TGCGCA 1 cut(s) 2943
GlaI GCGC 3 cut(s) 1288, 2915, 2943
GluI GCNGC 8 cut(s) 681, 684, 856, 1389, 1392, 1565, 2912, 2966
GsuI CTGGAG 2 cut(s) 1802, 2926
HaeIII GGCC 6 cut(s) 339, 1113, 1439, 1847, 2270, 2911
HapII CCGG 6 cut(s) 355, 1087, 1641, 2096, 2332, 2498
HgaI GACGC 4 cut(s) 921, 1602, 2309, 2850
HhaI GCGC 3 cut(s) 1289, 2916, 2944
Hin6I GCGC 3 cut(s) 1287, 2914, 2942
HinP1I GCGC 3 cut(s) 1287, 2914, 2942
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HindIII AAGCTT 2 cut(s) 17, 606
HpaII CCGG 6 cut(s) 355, 1087, 1641, 2096, 2332, 2498
HphI GGTGA 4 cut(s) 251, 948, 1016, 2822
Hpy166II GTNNAC 8 cut(s) 179, 213, 1094, 1296, 1636, 1865, 2411, 2899
Hpy8I GTNNAC 8 cut(s) 179, 213, 1094, 1296, 1636, 1865, 2411, 2899
Hpy99I CGWCG 1 cut(s) 2844
HpyAV CCTTC 3 cut(s) 351, 1908, 2468
HpyCH4III ACNGT 7 cut(s) 374, 838, 1263, 1977, 2302, 2920, 3007
HpyCH4IV ACGT 3 cut(s) 166, 1756, 2689
HpyF10VI GCNNNNNNNGC 3 cut(s) 864, 2476, 2651
HpyF3I CTNAG 7 cut(s) 881, 1699, 1767, 1853, 2213, 2367, 2778
HpySE526I ACGT 3 cut(s) 166, 1756, 2689
HspAI GCGC 3 cut(s) 1287, 2914, 2942
Kpn2I TCCGGA 2 cut(s) 1640, 2497
Ksp22I TGATCA 3 cut(s) 1045, 1456, 2428
LguI GCTCTTC 1 cut(s) 209
LmnI GCTCC 3 cut(s) 321, 1105, 1335
Lsp1109I GCAGC 4 cut(s) 667, 695, 1378, 2977
LweI GCATC 7 cut(s) 13, 667, 667, 1571, 2089, 2563, 2663
MabI ACCWGGT 1 cut(s) 980
MaeI CTAG 6 cut(s) 500, 518, 797, 821, 1983, 2892
MaeII ACGT 3 cut(s) 166, 1756, 2689
MaeIII GTNAC 9 cut(s) 117, 368, 782, 1009, 1019, 1824, 2111, 2604, 2621
MfeI CAATTG 1 cut(s) 1492
MflI RGATCY 3 cut(s) 143, 824, 1181
MhlI GDGCHC 3 cut(s) 675, 889, 2360
MlsI TGGCCA 2 cut(s) 1113, 1847
MluI ACGCGT 1 cut(s) 1243
MluNI TGGCCA 2 cut(s) 1113, 1847
MlyI GAGTC 3 cut(s) 1220, 1765, 2879
MmeI TCCRAC 4 cut(s) 901, 1001, 1855, 2108
Mox20I TGGCCA 2 cut(s) 1113, 1847
Mph1103I ATGCAT 1 cut(s) 1179
MroI TCCGGA 2 cut(s) 1640, 2497
MroXI GAANNNNTTC 3 cut(s) 189, 932, 1545
MscI TGGCCA 2 cut(s) 1113, 1847
MseI TTAA 9 cut(s) 140, 158, 635, 701, 1119, 1265, 2562, 2711, 2961
MslI CAYNNNNRTG 2 cut(s) 1158, 1469
Msp20I TGGCCA 2 cut(s) 1113, 1847
MspA1I CMGCKG 2 cut(s) 892, 2904
MspI CCGG 6 cut(s) 355, 1087, 1641, 2096, 2332, 2498
MspR9I CCNGG 6 cut(s) 728, 982, 1087, 1436, 1990, 2096
MunI CAATTG 1 cut(s) 1492
MvaI CCWGG 4 cut(s) 728, 982, 1436, 1990
MvnI CGCG 2 cut(s) 1245, 2914
MwoI GCNNNNNNNGC 3 cut(s) 864, 2476, 2651
NciI CCSGG 2 cut(s) 1087, 2096
NcoI CCATGG 1 cut(s) 2444
NdeI CATATG 1 cut(s) 538
NlaIV GGNNCC 3 cut(s) 323, 1183, 1337
NmuCI GTSAC 3 cut(s) 782, 2111, 2621
NsbI TGCGCA 1 cut(s) 2943
NsiI ATGCAT 1 cut(s) 1179
NspI RCATGY 3 cut(s) 1415, 1911, 2195
NspV TTCGAA 1 cut(s) 408
PaeI GCATGC 1 cut(s) 1911
PagI TCATGA 2 cut(s) 1153, 2071
PaqCI CACCTGC 2 cut(s) 992, 1105
PceI AGGCCT 1 cut(s) 1439
PciI ACATGT 1 cut(s) 1411
PciSI GCTCTTC 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 2571
PdmI GAANNNNTTC 3 cut(s) 189, 932, 1545
PflMI CCANNNNNTGG 3 cut(s) 566, 1109, 2589
PkrI GCNGC 8 cut(s) 682, 685, 857, 1390, 1393, 1566, 2913, 2967
PleI GAGTC 3 cut(s) 1219, 1765, 2878
PpsI GAGTC 3 cut(s) 1219, 1765, 2878
Ppu21I YACGTR 2 cut(s) 167, 1757
PscI ACATGT 1 cut(s) 1411
PshBI ATTAAT 1 cut(s) 1119
PsiI TTATAA 1 cut(s) 2511
Psp6I CCWGG 4 cut(s) 726, 980, 1434, 1988
PspGI CCWGG 4 cut(s) 726, 980, 1434, 1988
PspN4I GGNNCC 3 cut(s) 323, 1183, 1337
PspPI GGNCC 3 cut(s) 338, 1986, 2376
PstI CTGCAG 3 cut(s) 225, 685, 2728
PsuI RGATCY 3 cut(s) 143, 824, 1181
PvuII CAGCTG 2 cut(s) 892, 2904
RsaI GTAC 5 cut(s) 51, 1093, 1306, 1525, 2330
RsaNI GTAC 5 cut(s) 50, 1092, 1305, 1524, 2329
RseI CAYNNNNRTG 2 cut(s) 1158, 1469
SapI GCTCTTC 1 cut(s) 209
SaqAI TTAA 9 cut(s) 140, 158, 635, 701, 1119, 1265, 2562, 2711, 2961
SatI GCNGC 8 cut(s) 681, 684, 856, 1389, 1392, 1565, 2912, 2966
Sau96I GGNCC 3 cut(s) 338, 1986, 2376
SchI GAGTC 3 cut(s) 1220, 1765, 2879
ScrFI CCNGG 6 cut(s) 728, 982, 1087, 1436, 1990, 2096
SduI GDGCHC 3 cut(s) 675, 889, 2360
SexAI ACCWGGT 1 cut(s) 980
SfaNI GCATC 7 cut(s) 13, 667, 667, 1571, 2089, 2563, 2663
SfcI CTRYAG 4 cut(s) 221, 681, 1789, 2724
SfuI TTCGAA 1 cut(s) 408
SinI GGWCC 2 cut(s) 1986, 2376
SmiMI CAYNNNNRTG 2 cut(s) 1158, 1469
SmlI CTYRAG 5 cut(s) 416, 1187, 1883, 2116, 2180
SmoI CTYRAG 5 cut(s) 416, 1187, 1883, 2116, 2180
SnaBI TACGTA 2 cut(s) 167, 1757
SpeI ACTAGT 1 cut(s) 499
SphI GCATGC 1 cut(s) 1911
SseBI AGGCCT 1 cut(s) 1439
SsiI CCGC 5 cut(s) 856, 1388, 1564, 1905, 2912
SspI AATATT 2 cut(s) 762, 1915
SspMI CTAG 6 cut(s) 500, 518, 797, 821, 1983, 2892
StuI AGGCCT 1 cut(s) 1439
StyD4I CCNGG 6 cut(s) 726, 980, 1085, 1434, 1988, 2094
StyI CCWWGG 4 cut(s) 1839, 2124, 2444, 2568
TaaI ACNGT 7 cut(s) 374, 838, 1263, 1977, 2302, 2920, 3007
TaiI ACGT 3 cut(s) 169, 1759, 2692
TaqI TCGA 6 cut(s) 134, 256, 408, 432, 745, 2986
TatI WGTACW 1 cut(s) 1523
TauI GCSGC 4 cut(s) 858, 1391, 1567, 2914
Tru1I TTAA 9 cut(s) 140, 158, 635, 701, 1119, 1265, 2562, 2711, 2961
Tru9I TTAA 9 cut(s) 140, 158, 635, 701, 1119, 1265, 2562, 2711, 2961
TscAI CASTG 7 cut(s) 225, 922, 1476, 1653, 1950, 2173, 2307
TseFI GTSAC 3 cut(s) 782, 2111, 2621
TseI GCWGC 4 cut(s) 680, 683, 1391, 2965
Tsp45I GTSAC 3 cut(s) 782, 2111, 2621
TspGWI ACGGA 4 cut(s) 1797, 2255, 2396, 2918
TspRI CASTG 7 cut(s) 225, 922, 1476, 1653, 1950, 2173, 2307
Van91I CCANNNNNTGG 3 cut(s) 566, 1109, 2589
VpaK11BI GGWCC 2 cut(s) 1986, 2376
VspI ATTAAT 1 cut(s) 1119
XagI CCTNNNNNAGG 1 cut(s) 905
XceI RCATGY 3 cut(s) 1415, 1911, 2195
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 332
XmiI GTMKAC 2 cut(s) 178, 2410
XmnI GAANNNNTTC 3 cut(s) 189, 932, 1545
XspI CTAG 6 cut(s) 500, 518, 797, 821, 1983, 2892
Zsp2I ATGCAT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.