RchiOBHm_Chr6g0251641

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000307
Physical Location & Seq
Reverse (-)
6898866 .. 6907381
8516 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22559

Sequence Viewer

Length: 4743 bp
ATGTTTACCGTCCTCCTTTTCCTCATTCTCCTTGTTTCTCTTCTTCTTCAGTTTCTTAATTATCTTCTACCACCACCTGCCCAATCTCCAGCAGATTCCCCGAACATCCAAACACCTGCATCTCCTTCAAACCCTAAGTCAGAACATAAGGAAGTTCCAGCGGATTCCACAAACATCCAAATACCTGCATCTCCTTCGAACCCTAAATCAGAACATGATGAAGTTCCAGCGGCTTCCACAAACATCCAAATACTTGCATCTCCTTCAAACCCTAAATCAGAACATGAGGAAGTTCCAGCGGATTCCCCGAACATCCAAATACCTGCATCTCCTTCAACCCCTAAATTAGAACATGAGGAAGTTCCAGCAGATTCCACAAACACCCAAACAGTGCTTTCATCTCTTTCAGCCCCAATGGAACATGAGAAAGTTCCTCCAGCAGCTTCCTCTTCTTCGGGCTCAACATTGGAGCATGATGTTGTATCCCATGATGTCTTCCTCAGTTTTAGAGGTGAAGACACCCGCTATGCTTTTACTGACCATTTATATCATGCTATGAGGCGGCAAGGAATTAATGCATTCAGAGATACAGAAAAACTCGAGAGGGGACATTCAATTTCACCCGATCTCTTGAAAGCAATAAAAGAATCCCGAATTGCAGTCATCATTCTTTCAGCTAACTATGCTACTTCAACATGGTGTTTGGAGGAGCTTGCACAGATTATTGAATGCAAAGAGTCGACGGGGTTGAAAGTTTTACCAGTTTTCTATCACGTCGAACCATCAGAAGTAAGGAAGCAAACAGGAAATTTTCACCAGGCGTTTTGCAAGCATGAAGAAACTTTCGAAGACAACCCGGAGAAGGTGAAGAAGTGGAGAGAAGCTTTAAAACAAGTAGCCAATCTCTCCGGATGGACTTTGAAAGAAAGAGGAGAATCTGAAGTTATTCAGACAATAACTACAGAGATATTGAACACGTTGAATAACATGTGCTCAAGTGATAACAATGATTTAATTGGAATGGATGCTCGTATAGAGAAACTAATGTCTTACTTGGATCTGGGGTCGAATGATGTTCGCCCCGTAGGGATTGCCGGGATGGGCGGCATCGGAAAATCGACTCTGGCAAAGGCAGTTTTTGAAAAGATCCGTCACCAATTTGAATCTAGCTGCTTTATTAATATTGCTGACGTTAGAGGGAAATCCCTAGTTAAATTGCAGAAACTTCTCTGTCAAAATTTGCAGCTGGACAACCATGAAAGTATACAGAATGATTCTATGGGGATCAATTTATTAAGGAAACGATTAAGTCATAAAAGGGTGCTTATCATTCTAGATGATGTTGATCATACTAGTCAACTAGAAGCTCTCGTTGCCAAAGAAGACAAAGGAAAAAAGCCTTGTTTGGGTCCAGGGAGCAGAGTCATTATAACAACTAGAGATACACACAAGCTGAAGGAATTTGGATTACTGGAAGATGCTATATATGAGGCTGAGAAGTTGACTGACAAGGAAGCTCTTCAGCTTTTATGTCAGAAAGCTTTCAAGGAAAACCACGCTCCAGATGAATATAGGGAACTGTCCAATAGTTTTGTAAAATATTGCAGTGGCCTTCCTTTAGCTCTCAAAGTTCTGGGTTCTCACTTGTGTACGAGAACGGTTGATGAATGGTCAGCAGAATTGGCTAGACTTGATGAAGATCCCCACAAAGATATTTTTTCTATTCTTCAATTAAGTTTTGATGGATTGAAGAATACAGAGAAAGATATCTTTTTGGACATAGCATGTTTCTTCAGAGGCGAGAGTCAATATCGTGTAAGAAAGATTTTAGAGAGCTGTGACTTTCATCCTGGTGTCGGAATATCTGTCCTCGTTGAAAAACATTTAATTAATGTTGAGGAAGATAAACTGTGGATGCATGATTTGCTACAGCAAATGGGTTGGCACATTGTTCGGCAAGAATCTCCTGAGCCGGGAAAACGAAGCAGGTTGTGGCTTAATGAGAATTCCCATGAGTATCAACACAGCCGCCCGTGGTTTGATGAAGATACTCGTGATGTAGTCACCGAGAATCTGGGAATATATGCTGTCCAAGGGGTATATTTGAGCGTGCTGGAAAAAGAAAAGATACAATTGAAAGCTGACCCCTTCTTAAATATGCGCAAGCTAAGATTGTTGAAGATTTGTAATGTCAACTTGGTGGATGTGCGATTTGAATATCTGTCTAACAAGTTACGACTTTTGGAATGGCATGAATGTCCTCTAGAATCTCTGCCATCCAGTTTTCGACCAGATAAACTCGTCGAATTGAAACTGCCCAACAGCCGCATTGAAACACTGTGGAATCAAAGACTCTGTCTGGAGAAGCTAATACTGATGGATCTTTCCTACTGCCAATACTTGGTCAAGACCCCGGACTTCAGTAATTTCCCAAATCTTGTAGAGTTGACTTTTGAAGGTTGTAGAAAATTATCAGATGTTCACCCAACACTTTGGAGACTCAAAAATTTGATGATAGCAAATTTGAAAGATTGCACGAGTCTAGAGAGGCTTCCCAATTCCATCAGCTTGCAATCTCTTGCAATTCTTGTTCTTTCAGGATGTTCAAAACTAGATGAGTTTCCGGAGATAGTGGGAAACAGGGAAACTCTGGCAGCACTCTATTTAGATGGAACAGCTATAAGAGAGCTGCCGACAACAATCGGGTGCTTGAGTGGCCTAATTAGGTTAAATCTATGTGCCTGCAAGAACCTTGTGGGTCTTCCAAGCTTCATTTGCAGTTTGAAGTCTCTGAGATATCTGCGTCTGTCATATTCCTCACGCATTGACCAACTTCCAGAAGACATAGGGAGCTTGGAAAATTTGGAGAAGCTTGATGCTTGTTATACTGCTATAAGAAAAGTGCCTTCGTCCATTGTACTTCTGAAGAATCTGAAATTATTATGTTTCCATGGATGTAGGCAGGTGCCCGGGGAATGCTCTGATGCATCCTTGGGTTTGCAATTGCCTAAGTCGTTCGCCGGTTTGAGCTCATTGGAAACACTATCTTTAGGTCAATGCGATCTTGCTGAAGGAGCGATCCCTGATGACATTGGCTGCTTATCCTCACTGAAAGTTTTAAGGTTGGATGAAAACAATTTTGTGAGTCTACCTGAAAGCATCTCTCAACTTCCTAACCTTAGAGAAATTCACATCCCAAATTGTAGCAAGCTTCAGTCTTTGCCAAAACGTCTTCCAAAGGATGTTAATGTTGAGGCTAGTGGATGTCCCCTGCAGACAAACTATCCAAACCAGCTTAACATATGGCTTTCTGAGGAGGGGATCCGTTACATAAATTGTGGAAATTCAAAGCATGCAAGCTGTCCACTTCCAATTCCCGAAGAACAAATTGAAGAATACTTACCCAAGTACCTTGAGGATGAAATTATTCTTCAGCAGCACCTGCAGCTTCGTTACTTTCATACAGAAATTCCTGATTGGTGTCGTCACAAGAGTAGTGGGTCTTCTGTAGTAGCCATGCAAGTCTCTGATCCTGAAATTATTAAGAGCTTGGGAGTAGCCCTGTTTGTTGTTTTTGAAATCTTTGAGAGTGGTCACAATTTTGATAAGAGTTGGGAGTTGCAACAGACCCTTTGTACTTTTAAAGATGACCAAGGACTTGAACTTATCTATATCTTTAAAGAATTTGAAAAATTCCTGGCTGGAACACATGTATTATTTTGCTATGAGCCGCTAAAGTACTTTCACGTTGCTGGAATGTTGAATAAAGCAAGTCGACTTGAGGCTTCAGTTTCAACACAGAGACCAGATCTGGCGATTAAGTCTTGTGGGATGCAACTGATCTTTCAGGAAGAAGCTAAAGAGTTTGCTAAAAAATTAAGTGAGGCTTGTAATCCTCAGACGGATTTCGAATTTGGTAGACATTGTAAGGAATTGATAAAGGCCAAGGGAAAGAAAAGCTCCGGTAATATAATGGCACCAAATGAAGCAGAAAAGATTAGCTGGAAATTTGATTTTAACATGCACCAGGGAGAACATCTACAGTTGTTGCTTTCAAAAGTCTATGAGGATTTCAATCGGGACATCATATATAATGCAACTTACCCTCATTGCGCTAAACTTCTGGAGTGGTTCGGTGATCATCATCTCAGTAGGGGACCTTCAGTGAAAATCCCGTTACCACCATCTCTATCTACTGATGGGAATTGGATAGGATTGGCTCTATGTGCACGTTTTTCAGACCACGAGAATCAAACTACCTCCATAGACAAAGTGAATCCAGAAATTGCTGAGCTTGTTTGTCATTTGGAAACTGAGAACAGTGGTTTCGAACATCTTCACCACTATCGACCAACCAATGAAGAACTCAAGCGGTTATGTCCTACAGAATACATTTGGCTATCTTATATACCACGTCAGGGGTATCTAGATCAGTTAAATGAGTGTGACCTCTTGGAGGCTTCATTTGCCAGCGAAAGCCGAGGATTATTTGCTGAAGAATGTGGTCTCCGTCTTGTCTACCAGCATGATGAGCAAGAGTTTAAGAATCTGTGTACCTTCATGAACTCGTTTCTGGACAAAAGATCTGAAAGGTTGGAAGAATATGAGGCTCCCAAGAAGGGTAAACACGTTGTAGAATCTGATTTCAACATGCACCCGAGAGAAGATCTACAGTTGCTGCTTTCAGTGCTCTATAAGGTTATTACGGTCTCCCCCTCTCTCTGGTTTTTTTGTTTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1580

Amino Acids

179.26

Weight (kDa)

5.62

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 163 - 337 2.1e-56 TIR domain
TIR_2 PF13676 165 - 275 4.8e-16 TIR domain
NB-ARC PF00931 343 - 518 1.4e-22 NB-ARC domain
NACHT PF05729 361 - 512 9.1e-06 NACHT domain
WHD_ROQ1 PF23282 586 - 655 8.8e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 826 - 931 7e-07 Leucine-rich repeat region
LRR_13 PF23286 857 - 930 5.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 923 - 1019 3.5e-15 Leucine-rich repeat region
LRR_13 PF23286 940 - 1077 4.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1430
AarI CACCTGC 4 cut(s) 85, 124, 2953, 3450
Acc16I TGCGCA 1 cut(s) 2162
Acc36I ACCTGC 7 cut(s) 85, 124, 193, 331, 1977, 2953, 3450
AccB1I GGYRCC 2 cut(s) 2965, 3976
AccB7I CCANNNNNTGG 1 cut(s) 2401
AccI GTMKAC 6 cut(s) 740, 1264, 3148, 3775, 3919, 4518
AccIII TCCGGA 2 cut(s) 908, 2623
AclWI GGATC 9 cut(s) 1065, 1141, 1292, 1694, 2388, 3073, 3316, 3329, 3524
AfaI GTAC 6 cut(s) 1651, 2919, 3410, 3637, 3740, 4555
AfiI CCNNNNNNNGG 9 cut(s) 862, 1406, 1856, 1973, 2401, 4418, 4456, 4619, 4722
AflIII ACRYGT 4 cut(s) 975, 987, 3709, 4627
AhlI ACTAGT 1 cut(s) 1352
AjiI CACGTC 2 cut(s) 775, 4415
AjnI CCWGG 5 cut(s) 816, 1411, 1849, 3696, 4026
Alw21I GWGCWC 4 cut(s) 995, 3032, 4231, 4692
Alw26I GTCTC 6 cut(s) 2491, 2793, 3529, 3796, 4511, 4714
Alw44I GTGCAC 1 cut(s) 4227
AlwI GGATC 9 cut(s) 1065, 1141, 1292, 1694, 2388, 3073, 3316, 3329, 3524
AlwNI CAGNNNCTG 2 cut(s) 3442, 4678
Ama87I CYCGRG 3 cut(s) 599, 2969, 4657
Aor13HI TCCGGA 2 cut(s) 908, 2623
AoxI GGCC 3 cut(s) 1609, 2716, 3942
ApaLI GTGCAC 1 cut(s) 4227
ApeKI GCWGC 9 cut(s) 440, 1170, 1243, 2654, 2689, 3096, 3436, 3445, 4678
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 2341, 2373
AseI ATTAAT 3 cut(s) 573, 1179, 1890
Asp700I GAANNNNTTC 5 cut(s) 945, 1518, 1541, 3426, 4335
AspLEI GCGC 2 cut(s) 2163, 4115
AspS9I GGNCC 2 cut(s) 1409, 4157
AsuC2I CCSGG 6 cut(s) 857, 1096, 1974, 2414, 2970, 2971
AsuHPI GGTGA 9 cut(s) 524, 612, 806, 877, 1145, 2056, 2474, 4148, 4331
AsuII TTCGAA 4 cut(s) 197, 846, 3909, 4329
AvaI CYCGRG 3 cut(s) 599, 2969, 4657
AvaII GGWCC 2 cut(s) 1409, 4157
BaeGI GKGCMC 2 cut(s) 2970, 4231
BaeI ACNNNNGTAYC 2 cut(s) 3392, 3425
BamHI GGATCC 1 cut(s) 3321
BanI GGYRCC 2 cut(s) 2965, 3976
BanII GRGCYC 2 cut(s) 461, 3032
BarI GAAGNNNNNNTAC 6 cut(s) 3384, 3416, 3487, 3519, 4144, 4176
BauI CACGAG 3 cut(s) 2052, 2536, 4244
BbsI GAAGAC 8 cut(s) 487, 522, 855, 1389, 2753, 2847, 3224, 3495
Bbv12I GWGCWC 4 cut(s) 995, 3032, 4231, 4692
BbvI GCAGC 9 cut(s) 452, 1157, 1255, 2666, 2676, 3083, 3448, 3457, 4665
BcgI CGANNNNNNTGC 4 cut(s) 177, 211, 1934, 1968
BciT130I CCWGG 5 cut(s) 818, 1413, 1851, 3698, 4028
BciVI GTATCC 1 cut(s) 493
BclI TGATCA 2 cut(s) 1345, 4138
BcnI CCSGG 6 cut(s) 857, 1096, 1974, 2414, 2970, 2971
BcoDI GTCTC 6 cut(s) 2491, 2793, 3529, 3796, 4511, 4714
BcuI ACTAGT 1 cut(s) 1352
BfmI CTRYAG 8 cut(s) 960, 1928, 3272, 3443, 3507, 4040, 4383, 4670
BfuAI ACCTGC 7 cut(s) 85, 124, 193, 331, 1977, 2953, 3450
BfuI GTATCC 1 cut(s) 493
BglII AGATCT 3 cut(s) 3808, 4583, 4666
BlpI GCTNAGC 1 cut(s) 4290
BmcAI AGTACT 1 cut(s) 3740
Bme18I GGWCC 2 cut(s) 1409, 4157
BmeT110I CYCGRG 3 cut(s) 599, 2969, 4657
BmgBI CACGTC 2 cut(s) 775, 4415
BmgT120I GGNCC 2 cut(s) 1409, 4157
BmiI GGNNCC 6 cut(s) 1410, 2967, 3323, 3978, 4158, 4611
BpiI GAAGAC 8 cut(s) 487, 522, 855, 1389, 2753, 2847, 3224, 3495
BpmI CTGGAG 5 cut(s) 72, 420, 1545, 2381, 4145
Bpu10I CCTNAGC 1 cut(s) 1968
Bpu1102I GCTNAGC 1 cut(s) 4290
Bpu14I TTCGAA 4 cut(s) 197, 846, 3909, 4329
BpuEI CTTGAG 5 cut(s) 979, 2731, 3434, 3800, 4352
BpuMI CCSGG 6 cut(s) 857, 1096, 1974, 2414, 2970, 2971
BsaBI GATNNNNATC 4 cut(s) 1344, 1698, 4074, 4143
BsaI GGTCTC 3 cut(s) 3796, 4511, 4714
BsaWI WCCGGW 3 cut(s) 908, 2623, 3962
Bsc4I CCNNNNNNNGG 9 cut(s) 862, 1406, 1856, 1973, 2401, 4418, 4456, 4619, 4722
Bse118I RCCGGY 1 cut(s) 3020
Bse1I ACTGG 3 cut(s) 761, 1476, 2280
Bse3DI GCAATG 1 cut(s) 4108
Bse8I GATNNNNATC 4 cut(s) 1344, 1698, 4074, 4143
BseAI TCCGGA 2 cut(s) 908, 2623
BseBI CCWGG 5 cut(s) 818, 1413, 1851, 3698, 4028
BseJI GATNNNNATC 4 cut(s) 1344, 1698, 4074, 4143
BseLI CCNNNNNNNGG 9 cut(s) 862, 1406, 1856, 1973, 2401, 4418, 4456, 4619, 4722
BseMI GCAATG 1 cut(s) 4108
BseMII CTCAG 9 cut(s) 514, 1485, 1959, 2783, 3303, 3911, 4162, 4281, 4305
BseNI ACTGG 3 cut(s) 761, 1476, 2280
BseRI GAGGAG 3 cut(s) 722, 945, 3329
BseSI GKGCMC 2 cut(s) 2970, 4231
BseXI GCAGC 9 cut(s) 452, 1157, 1255, 2666, 2676, 3083, 3448, 3457, 4665
BshFI GGCC 3 cut(s) 1611, 2718, 3944
BshNI GGYRCC 2 cut(s) 2965, 3976
BsiHKAI GWGCWC 4 cut(s) 995, 3032, 4231, 4692
BsiHKCI CYCGRG 3 cut(s) 599, 2969, 4657
BsiSI CCGG 9 cut(s) 857, 909, 1095, 1973, 2414, 2624, 2970, 3021, 3963
BslFI GGGAC 4 cut(s) 621, 3252, 4094, 4170
BslI CCNNNNNNNGG 9 cut(s) 862, 1406, 1856, 1973, 2401, 4418, 4456, 4619, 4722
BsmAI GTCTC 6 cut(s) 2491, 2793, 3529, 3796, 4511, 4714
BsmFI GGGAC 4 cut(s) 621, 3252, 4094, 4170
BsmI GAATGC 3 cut(s) 578, 734, 2981
BsnI GGCC 3 cut(s) 1611, 2718, 3944
Bso31I GGTCTC 3 cut(s) 3796, 4511, 4714
BsoBI CYCGRG 3 cut(s) 599, 2969, 4657
Bsp119I TTCGAA 4 cut(s) 197, 846, 3909, 4329
Bsp1286I GDGCHC 6 cut(s) 461, 995, 2970, 3032, 4231, 4692
Bsp13I TCCGGA 2 cut(s) 908, 2623
Bsp1720I GCTNAGC 1 cut(s) 4290
Bsp19I CCATGG 1 cut(s) 2950
BspANI GGCC 3 cut(s) 1611, 2718, 3944
BspCNI CTCAG 9 cut(s) 513, 1486, 1960, 2784, 3304, 3910, 4161, 4282, 4306
BspEI TCCGGA 2 cut(s) 908, 2623
BspHI TCATGA 1 cut(s) 4560
BspLI GGNNCC 6 cut(s) 1410, 2967, 3323, 3978, 4158, 4611
BspMAI CTGCAG 2 cut(s) 3276, 3447
BspMI ACCTGC 7 cut(s) 85, 124, 193, 331, 1977, 2953, 3450
BspPI GGATC 9 cut(s) 1065, 1141, 1292, 1694, 2388, 3073, 3316, 3329, 3524
BspQI GCTCTTC 1 cut(s) 1524
BspT104I TTCGAA 4 cut(s) 197, 846, 3909, 4329
BspT107I GGYRCC 2 cut(s) 2965, 3976
BspTNI GGTCTC 3 cut(s) 3796, 4511, 4714
BsrDI GCAATG 1 cut(s) 4108
BsrFI RCCGGY 1 cut(s) 3020
BsrI ACTGG 3 cut(s) 761, 1476, 2280
BssAI RCCGGY 1 cut(s) 3020
BssNAI GTATAC 1 cut(s) 1265
BssSI CACGAG 3 cut(s) 2052, 2536, 4244
BssT1I CCWWGG 5 cut(s) 2092, 2950, 2991, 3652, 3945
Bst1107I GTATAC 1 cut(s) 1265
Bst2BI CACGAG 3 cut(s) 2052, 2536, 4244
Bst2UI CCWGG 5 cut(s) 818, 1413, 1851, 3698, 4028
Bst6I CTCTTC 3 cut(s) 45, 454, 1524
BstAPI GCANNNNNTGC 2 cut(s) 1924, 3442
BstBI TTCGAA 4 cut(s) 197, 846, 3909, 4329
BstDSI CCRYGG 2 cut(s) 2033, 2950
BstENI CCTNNNNNAGG 1 cut(s) 4454
BstHHI GCGC 2 cut(s) 2163, 4115
BstMAI GTCTC 6 cut(s) 2491, 2793, 3529, 3796, 4511, 4714
BstNI CCWGG 5 cut(s) 818, 1413, 1851, 3698, 4028
BstNSI RCATGY 6 cut(s) 991, 1788, 3356, 3713, 4024, 4654
BstSFI CTRYAG 8 cut(s) 960, 1928, 3272, 3443, 3507, 4040, 4383, 4670
BstSLI GKGCMC 2 cut(s) 2970, 4231
BstV1I GCAGC 9 cut(s) 452, 1157, 1255, 2666, 2676, 3083, 3448, 3457, 4665
BstV2I GAAGAC 8 cut(s) 487, 522, 855, 1389, 2753, 2847, 3224, 3495
BstX2I RGATCY 8 cut(s) 1057, 1146, 1699, 2380, 3321, 3808, 4583, 4666
BstXI CCANNNNNNTGG 1 cut(s) 2493
BstYI RGATCY 8 cut(s) 1057, 1146, 1699, 2380, 3321, 3808, 4583, 4666
BstZ17I GTATAC 1 cut(s) 1265
BsuI GTATCC 1 cut(s) 493
BsuRI GGCC 3 cut(s) 1611, 2718, 3944
BtgI CCRYGG 2 cut(s) 2033, 2950
BtrI CACGTC 2 cut(s) 775, 4415
BtsI GCAGTG 1 cut(s) 1612
BtsIMutI CAGTG 7 cut(s) 396, 1612, 2336, 3107, 4170, 4327, 4692
BveI ACCTGC 7 cut(s) 85, 124, 193, 331, 1977, 2953, 3450
CaiI CAGNNNCTG 2 cut(s) 3442, 4678
CciI TCATGA 1 cut(s) 4560
CfoI GCGC 2 cut(s) 2163, 4115
Cfr10I RCCGGY 1 cut(s) 3020
Cfr13I GGNCC 2 cut(s) 1409, 4157
Cfr9I CCCGGG 1 cut(s) 2969
CseI GACGC 1 cut(s) 2792
Csp6I GTAC 6 cut(s) 1650, 2918, 3409, 3636, 3739, 4554
CspCI CAANNNNNGTGG 2 cut(s) 3478, 3513
CviQI GTAC 6 cut(s) 1650, 2918, 3409, 3636, 3739, 4554
DraI TTTAAA 3 cut(s) 888, 3643, 3679
Eam1104I CTCTTC 3 cut(s) 45, 454, 1524
EarI CTCTTC 3 cut(s) 45, 454, 1524
Ecl136II GAGCTC 1 cut(s) 3030
Eco130I CCWWGG 5 cut(s) 2092, 2950, 2991, 3652, 3945
Eco24I GRGCYC 2 cut(s) 461, 3032
Eco31I GGTCTC 3 cut(s) 3796, 4511, 4714
Eco32I GATATC 2 cut(s) 1768, 2798
Eco47I GGWCC 2 cut(s) 1409, 4157
Eco53kI GAGCTC 1 cut(s) 3030
Eco88I CYCGRG 3 cut(s) 599, 2969, 4657
EcoICRI GAGCTC 1 cut(s) 3030
EcoNI CCTNNNNNAGG 1 cut(s) 4454
EcoO109I RGGNCCY 1 cut(s) 4157
EcoRI GAATTC 1 cut(s) 2005
EcoRII CCWGG 5 cut(s) 816, 1411, 1849, 3696, 4026
EcoRV GATATC 2 cut(s) 1768, 2798
EcoT14I CCWWGG 5 cut(s) 2092, 2950, 2991, 3652, 3945
EcoT22I ATGCAT 3 cut(s) 580, 1920, 2989
EcoT38I GRGCYC 2 cut(s) 461, 3032
ErhI CCWWGG 5 cut(s) 2092, 2950, 2991, 3652, 3945
FalI AAGNNNNNCTT 2 cut(s) 3871, 3903
FaqI GGGAC 4 cut(s) 621, 3252, 4094, 4170
FauI CCCGC 1 cut(s) 530
FauNDI CATATG 1 cut(s) 3302
FbaI TGATCA 2 cut(s) 1345, 4138
FblI GTMKAC 6 cut(s) 740, 1264, 3148, 3775, 3919, 4518
FriOI GRGCYC 2 cut(s) 461, 3032
FspI TGCGCA 1 cut(s) 2162
GlaI GCGC 2 cut(s) 2162, 4114
GsuI CTGGAG 5 cut(s) 72, 420, 1545, 2381, 4145
HaeIII GGCC 3 cut(s) 1611, 2718, 3944
HapII CCGG 9 cut(s) 857, 909, 1095, 1973, 2414, 2624, 2970, 3021, 3963
HgaI GACGC 1 cut(s) 2792
HhaI GCGC 2 cut(s) 2163, 4115
Hin6I GCGC 2 cut(s) 2161, 4113
HinP1I GCGC 2 cut(s) 2161, 4113
HincII GTYRAC 6 cut(s) 741, 1358, 1503, 2194, 2448, 3776
HindII GTYRAC 6 cut(s) 741, 1358, 1503, 2194, 2448, 3776
HindIII AAGCTT 5 cut(s) 882, 1539, 2767, 2870, 3209
HpaII CCGG 9 cut(s) 857, 909, 1095, 1973, 2414, 2624, 2970, 3021, 3963
HphI GGTGA 9 cut(s) 524, 612, 806, 877, 1145, 2056, 2474, 4148, 4331
Hpy99I CGWCG 3 cut(s) 745, 779, 2306
HpyCH4IV ACGT 8 cut(s) 774, 977, 1191, 3229, 3747, 4231, 4414, 4629
HpySE526I ACGT 8 cut(s) 774, 977, 1191, 3229, 3747, 4231, 4414, 4629
HspAI GCGC 2 cut(s) 2161, 4113
Kpn2I TCCGGA 2 cut(s) 908, 2623
Ksp22I TGATCA 2 cut(s) 1345, 4138
LguI GCTCTTC 1 cut(s) 1524
LmnI GCTCC 8 cut(s) 469, 709, 1416, 1564, 2850, 3074, 3965, 4615
Lsp1109I GCAGC 9 cut(s) 452, 1157, 1255, 2666, 2676, 3083, 3448, 3457, 4665
MaeII ACGT 8 cut(s) 774, 977, 1191, 3229, 3747, 4231, 4414, 4629
MfeI CAATTG 2 cut(s) 2132, 3002
MflI RGATCY 8 cut(s) 1057, 1146, 1699, 2380, 3321, 3808, 4583, 4666
MhlI GDGCHC 6 cut(s) 461, 995, 2970, 3032, 4231, 4692
MlyI GAGTC 8 cut(s) 746, 1114, 1431, 1813, 2346, 2493, 2548, 3154
MmeI TCCRAC 3 cut(s) 1837, 3105, 4575
Mph1103I ATGCAT 3 cut(s) 580, 1920, 2989
MroI TCCGGA 2 cut(s) 908, 2623
MroXI GAANNNNTTC 5 cut(s) 945, 1518, 1541, 3426, 4335
MslI CAYNNNNRTG 1 cut(s) 1851
MspA1I CMGCKG 4 cut(s) 161, 230, 299, 1246
MspI CCGG 9 cut(s) 857, 909, 1095, 1973, 2414, 2624, 2970, 3021, 3963
MunI CAATTG 2 cut(s) 2132, 3002
Mva1269I GAATGC 3 cut(s) 578, 734, 2981
MvaI CCWGG 5 cut(s) 818, 1413, 1851, 3698, 4028
NciI CCSGG 6 cut(s) 857, 1096, 1974, 2414, 2970, 2971
NcoI CCATGG 1 cut(s) 2950
NdeI CATATG 1 cut(s) 3302
NlaIV GGNNCC 6 cut(s) 1410, 2967, 3323, 3978, 4158, 4611
NmeAIII GCCGAG 1 cut(s) 4505
NmuCI GTSAC 6 cut(s) 1151, 1838, 2062, 3485, 3593, 4445
NsbI TGCGCA 1 cut(s) 2162
NsiI ATGCAT 3 cut(s) 580, 1920, 2989
NspI RCATGY 6 cut(s) 991, 1788, 3356, 3713, 4024, 4654
NspV TTCGAA 4 cut(s) 197, 846, 3909, 4329
PacI TTAATTAA 1 cut(s) 1890
PaeI GCATGC 1 cut(s) 3356
PaeR7I CTCGAG 1 cut(s) 599
PagI TCATGA 1 cut(s) 4560
PaqCI CACCTGC 4 cut(s) 85, 124, 2953, 3450
PciI ACATGT 2 cut(s) 987, 3709
PciSI GCTCTTC 1 cut(s) 1524
PctI GAATGC 3 cut(s) 578, 734, 2981
PdmI GAANNNNTTC 5 cut(s) 945, 1518, 1541, 3426, 4335
PflFI GACNNNGTC 1 cut(s) 2355
PflMI CCANNNNNTGG 1 cut(s) 2401
PleI GAGTC 8 cut(s) 745, 1114, 1430, 1812, 2346, 2493, 2547, 3153
PpsI GAGTC 8 cut(s) 745, 1114, 1430, 1812, 2346, 2493, 2547, 3153
PpuMI RGGWCCY 1 cut(s) 4157
PscI ACATGT 2 cut(s) 987, 3709
PshBI ATTAAT 3 cut(s) 573, 1179, 1890
PsiI TTATAA 1 cut(s) 1430
Psp124BI GAGCTC 1 cut(s) 3032
Psp5II RGGWCCY 1 cut(s) 4157
Psp6I CCWGG 5 cut(s) 816, 1411, 1849, 3696, 4026
PspGI CCWGG 5 cut(s) 816, 1411, 1849, 3696, 4026
PspN4I GGNNCC 6 cut(s) 1410, 2967, 3323, 3978, 4158, 4611
PspPI GGNCC 2 cut(s) 1409, 4157
PspPPI RGGWCCY 1 cut(s) 4157
PstI CTGCAG 2 cut(s) 3276, 3447
PstNI CAGNNNCTG 2 cut(s) 3442, 4678
PsuI RGATCY 8 cut(s) 1057, 1146, 1699, 2380, 3321, 3808, 4583, 4666
PsyI GACNNNGTC 1 cut(s) 2355
PvuII CAGCTG 1 cut(s) 1246
RsaI GTAC 6 cut(s) 1651, 2919, 3410, 3637, 3740, 4555
RsaNI GTAC 6 cut(s) 1650, 2918, 3409, 3636, 3739, 4554
RseI CAYNNNNRTG 1 cut(s) 1851
SacI GAGCTC 1 cut(s) 3032
SalI GTCGAC 2 cut(s) 739, 3774
SapI GCTCTTC 1 cut(s) 1524
Sau96I GGNCC 2 cut(s) 1409, 4157
ScaI AGTACT 1 cut(s) 3740
SchI GAGTC 8 cut(s) 746, 1114, 1431, 1813, 2346, 2493, 2548, 3154
SduI GDGCHC 6 cut(s) 461, 995, 2970, 3032, 4231, 4692
SfcI CTRYAG 8 cut(s) 960, 1928, 3272, 3443, 3507, 4040, 4383, 4670
Sfr274I CTCGAG 1 cut(s) 599
SfuI TTCGAA 4 cut(s) 197, 846, 3909, 4329
SinI GGWCC 2 cut(s) 1409, 4157
SlaI CTCGAG 1 cut(s) 599
SmaI CCCGGG 1 cut(s) 2971
SmiMI CAYNNNNRTG 1 cut(s) 1851
SmlI CTYRAG 6 cut(s) 599, 994, 2710, 3413, 3779, 4367
SmoI CTYRAG 6 cut(s) 599, 994, 2710, 3413, 3779, 4367
SpeI ACTAGT 1 cut(s) 1352
SphI GCATGC 1 cut(s) 3356
SspI AATATT 2 cut(s) 1183, 1601
SstI GAGCTC 1 cut(s) 3032
StyI CCWWGG 5 cut(s) 2092, 2950, 2991, 3652, 3945
TaiI ACGT 8 cut(s) 777, 980, 1194, 3232, 3750, 4234, 4417, 4632
TatI WGTACW 3 cut(s) 2917, 3635, 3738
TauI GCSGC 6 cut(s) 233, 565, 1107, 2031, 2328, 3733
TscAI CASTG 7 cut(s) 396, 1612, 2343, 3114, 4170, 4327, 4692
TseFI GTSAC 6 cut(s) 1151, 1838, 2062, 3485, 3593, 4445
TseI GCWGC 9 cut(s) 440, 1170, 1243, 2654, 2689, 3096, 3436, 3445, 4678
Tsp45I GTSAC 6 cut(s) 1151, 1838, 2062, 3485, 3593, 4445
TspGWI ACGGA 4 cut(s) 1139, 3314, 3917, 4499
TspMI CCCGGG 1 cut(s) 2969
TspRI CASTG 7 cut(s) 396, 1612, 2343, 3114, 4170, 4327, 4692
Tth111I GACNNNGTC 1 cut(s) 2355
Van91I CCANNNNNTGG 1 cut(s) 2401
VneI GTGCAC 1 cut(s) 4227
VpaK11BI GGWCC 2 cut(s) 1409, 4157
VspI ATTAAT 3 cut(s) 573, 1179, 1890
XagI CCTNNNNNAGG 1 cut(s) 4454
XbaI TCTAGA 4 cut(s) 1333, 2263, 2542, 4426
XceI RCATGY 6 cut(s) 991, 1788, 3356, 3713, 4024, 4654
XhoI CTCGAG 1 cut(s) 599
XmaI CCCGGG 1 cut(s) 2969
XmiI GTMKAC 6 cut(s) 740, 1264, 3148, 3775, 3919, 4518
XmnI GAANNNNTTC 5 cut(s) 945, 1518, 1541, 3426, 4335
ZrmI AGTACT 1 cut(s) 3740
Zsp2I ATGCAT 3 cut(s) 580, 1920, 2989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.