Rw2G014610

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000307
Physical Location & Seq
Reverse (-)
16445859 .. 16452053
6195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014610.1

Sequence Viewer

Length: 3627 bp
ATGTCATGGCGTTTTGAGGATGTTCGCAACGTGCTGATGGACAATACGGGAACAACTACTGTTGAAGGCTTATTCTTAAGCTCGCCTGAAAAAGAAGAAATGCCATTGGATGATGACCCGTTCTCAATTATGTCTCTAGAATCTCTGCCATCTAATTTTAAATCGGACAAGCTGGTTGAATTCAAGATGCCTGACAGCCGCATTAAGAAACTATGGAATGAAACGCTCTCTCTTAGATTGCTACTACTCATGGATCTTTCCAACTGCCAATATTTAGCCACGACCCCGGACTTCAGTAAGGTTCCAAATCTTGAGAGACTGATCCTTGAAGGTTGTACTGAGTTATCAATAGTTCACCCAACAATTGGGGATCTCCAGCAACTGGTTTTTTTGAATCTGAAAGGTTGTGCAAGTCTGGAGAGCCTTCCTCACTCCATCAGGTTGAGATCTCTTCGAATTTTTATTCTTTCAGGATGTTCAAAACTTAAACAGTTTCCAGAGATTGTGGGGAACATGGATGCATTATCTGAACTTTATTTAGATGGCTCGGCTATACGGGAGCTGCCTGTATCAATCCAGCATTTGGATGGCCTTGTTTTGCTAAATCTTAGCGGCTGCAGGAACCTTCTGAGTCTTCCTAGTGTTCTTTGTAGTCATTTGATATCACTGAAATTTCTCTATCTGTCATTGTGCTCAAGCATGGAAAAACTGCCAGAAAACATAGGCTACTTGAAACAGTTGGAGGAGCTTGATGCTTGTGATACTGCTATAAGAAAAGTTCCCGAGTCCATTTCAGGTCTCAAGAATCTAGAACTATTGTGCTTCCATGGATGTTCTGGTTTCACAGGTTTAGAATTGCCGAACAAGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGTGGATGTAATCTCGCAGAAGGAGCAATCCCCCCTGACATTGGTGATTTATTCTCACTGCAGAGTCTGGATTTAAGTGAGAACAACTTTTTCACCATACCTGAAAGCATCTCTCAGCTTTCTGAGCTTACAGAAATTTCTTTGTTTAAGTGTAGCAAACTACGGTCGTTGCCAAAGGATCTTCCGTCAAGTCTAAGAAATGTAAATATACGTGGTTGTCCTATGCTGACAAATTCTTCATCTAGTTGGAGGAGATATCCGCCTCAAAAGGGTTTGAGTATCATAAACTGTCGAAAACCAGAGGAGGATGACATCTTTCCTGAACTCCATAAGTTTCGTTTGGGAAATCTGGAAGAACTCGATCTTTCCTACTGCCAACACTTGAAGAAGATCCCTGACTTGAATGGGGTTCCACATCTTAAGAAATTGAACCTTGAAGGTTGTGAAAAATTATCAGAGATTCACTCAACAATTGGGCGTCTCCAACATCTGATTTTTTTGAATTTGAGAGGATGTGTAGATCTAGAGAGCCTTCCCTGCTATATTAGCCTGAAATCTCTTAAAATTCTTATTCTCTCGGGATGTTCAAAACTTAAAGAGTTTCCAGACACTAAGGGAAATATGGAGAATTTTTCACAACTGCATTTAGATGGGACGGCGTTAAAGTATCTGCCAATACCGATGAAGCAATTGAAATGCCCTATTTCGATAAACCTAAGAGGCTGCAGGAACCTTTTGAGTGTTCCAGTCCTATTAAGTCTGAAATCCCTCAATCTGTCGGGCTGCTCACGTATATCCAGATTGCCACTCAATCTGGGAACCATGGCACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACAGAATTACCCCTGTCCTTTTCATTTCTGGGGAAGCTTAAAGTGTTATCTTTCTGTGGATGTAAAGGTTTGCAGTTGGCTAACTGGTCCGTCGACTTAAACTTTTTGACATCATTAAATCTACAGAGGTGTGGTCTAGCAGAAGAAGTCTTTGATAGCCTCTACGGCTTGTCCTCTTTGCAGATTTTGGATCTCAGTGCAAACAATATTTTGTTCATACCTAATGAAATCGGTCGCTTATCCTCTTTGCAGCGATTGCATTTGAGTGGGAACAAATTTGTGAGTATACCAAATGAAATTGGTGACTTGTCCTCTTTGCAGCTTTTAGATTTGAGTGACAACAACTTCATGAGTATACCTGGGAGCATCTCTCATCTTTCTAAGCTTACAGAACTCCGCCTGTTTAGATGTAGTAAGCTACAGTCCTTGCCAAATTATCTTCCCTTCAATCTAAAACATGTTTATGCACAAGAATGTCCTATGTTGAAGTATAATGCAGATACTTTGACAATATGGGCATCCGGAAAAGGGTTCTGTTTCATGCATTGCAGAAAATCTGAGCAGGATGATGATCAGCCAAGCCACGTCCCAGTTCCAGTTCCTGAAGACCGTATCGAACTACTCTTTCCTATATACATTGAGGACCGAGTGTATGGTAAAAAGCCATTTGAAATTCGTTTTCCACACGGTACTAGAATTCCCAATCTGTGGAGTCATTGGAGCAGTGGCCCTTCTGTGACAATCCCACTATCTGATGGTAACAGTGCGTGTATGGGACTTGCTCTGTTTGTTGTTTTTGAAATCCTTGAGAAAGGCAATTTCAACAAGATTTGGGAATGGGAGGACACTATTGGACCCAATGGAGGTGAGAAGAGTTTTCATCTTTGTGTTCCTGCTCCGATAGTTTCAATTCCAACCTGGTTCCTCAATCATGATGCAGGGGATGTAACACTTTGTCACATTACTGAAAACTTGCTTCATGATCAGGCATGGGTGGGATTAGAACTTTATGTCGGATTTACTCGACGTGCATCTGCTGTGAGACATGGCAGTGATAGTTTCTTCTTTAACGTTGATTTCTGTGCTTATGATCATGAAAGTGTAGTAATGCATCAATCCCTCGAGATTAATAGTTGTCTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGCATTCATTTTCCACAACACTTGAATCAATTTAAGGGTATTAGCGCATTGTTCAGAACCGTTAACCCTGAAATGGAGGTCCAAATGTGTGGGAGCCGTTTAGCCTTCGAGCACGATCTTGAAGACTTGACCAAATCATTAACTGAGGTTGCAGGCATGCTTGGAGAACATGTCCGCACTCAGATTTGCTCCCTAGGCCAGCGCAATGCAGTGGAAGCAGAAATGCCCATCAATTGCTGTTCCTGGTTCAGAAGAAGTGCTGCACTAGTACTACCAAAACAAGCTCCTGCTCCTTCTTCCTCAATAGTGCGGCTGCAAAATCATAGATGCTCTATCCAGCAACAGCAAGAGTTAAATGTGGTTAATGATGTTGAGGCTAGAATTTATGTTAGTTCCCCAGGCTTGAGAGTGGACAGTTGTGGTATCCGTCTCTTGTATGGGCATGAAGAGAGCGAATTTAACAGCCTAGTAACGGAGAGCTGGACCTCTTTCTTTGATAATCTGCGTCTCATTCGTCAACTGGTAGAAGCAGATGATGAAAATGTTCAGCCATGGACAAGGCATGAACTTCCGATGCTTGAAGGGCATATCAAGTTTGAGCCTCCGAGGCAAAGACCAAAGCCCGGTGTAGCTTATCTTGGACCAGTCGAGAAGGCCCATATTAACCCAGGACTGAGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

1208

Amino Acids

135.47

Weight (kDa)

5.87

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 120 - 225 1.3e-06 Leucine-rich repeat region
LRR_13 PF23286 148 - 216 3.8e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 200 - 301 9e-12 Leucine-rich repeat region
LRR_14 PF23598 405 - 517 1.1e-07 Leucine-rich repeat region
LRR_4 PF12799 417 - 455 7.8e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 543 - 656 2.4e-08 Leucine-rich repeat region
LRR_8 PF13855 625 - 682 3.9e-07 Leucine rich repeat
LRR_14 PF23598 664 - 729 3.6e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 4 cut(s) 365, 382, 583, 2475
AccI GTMKAC 3 cut(s) 1860, 2053, 2122
AccIII TCCGGA 1 cut(s) 2288
AciI CCGC 6 cut(s) 199, 612, 1163, 2164, 3118, 3253
AclI AACGTT 1 cut(s) 2838
AclWI GGATC 6 cut(s) 261, 316, 378, 1089, 1288, 1965
AcuI CTGAAG 2 cut(s) 277, 2391
AcyI GRCGYC 1 cut(s) 1381
AfaI GTAC 4 cut(s) 337, 2458, 2940, 3213
AflII CTTAAG 2 cut(s) 76, 1322
AflIII ACRYGT 3 cut(s) 2224, 2935, 3112
AhlI ACTAGT 1 cut(s) 3208
AjiI CACGTC 2 cut(s) 2353, 2795
AjnI CCWGG 5 cut(s) 2125, 2684, 3185, 3340, 3610
Alw21I GWGCWC 2 cut(s) 695, 3057
Alw26I GTCTC 7 cut(s) 138, 310, 803, 1388, 2803, 3377, 3455
AlwI GGATC 6 cut(s) 261, 316, 378, 1089, 1288, 1965
AlwNI CAGNNNCTG 3 cut(s) 382, 970, 2369
Ama87I CYCGRG 3 cut(s) 782, 1480, 2888
Aor13HI TCCGGA 1 cut(s) 2288
AoxI GGCC 4 cut(s) 589, 2494, 3139, 3597
ApeKI GCWGC 8 cut(s) 562, 615, 1626, 1686, 2017, 2086, 3203, 3256
AseI ATTAAT 1 cut(s) 2895
Asp700I GAANNNNTTC 2 cut(s) 866, 3486
AspA2I CCTAGG 2 cut(s) 901, 3136
AspLEI GCGC 2 cut(s) 2990, 3147
AspS9I GGNCC 9 cut(s) 1854, 2410, 2495, 2621, 2910, 3022, 3426, 3584, 3598
AsuC2I CCSGG 2 cut(s) 287, 3567
AsuHPI GGTGA 5 cut(s) 347, 959, 988, 2081, 2645
AsuII TTCGAA 1 cut(s) 454
AvaI CYCGRG 3 cut(s) 782, 1480, 2888
AvaII GGWCC 7 cut(s) 1854, 2410, 2621, 2910, 3022, 3426, 3584
AvrII CCTAGG 2 cut(s) 901, 3136
BbsI GAAGAC 3 cut(s) 626, 2379, 3072
Bbv12I GWGCWC 2 cut(s) 695, 3057
BbvI GCAGC 8 cut(s) 549, 602, 1613, 1673, 2029, 2098, 3190, 3243
BccI CCATC 8 cut(s) 31, 157, 443, 536, 581, 1547, 2516, 3179
BceAI ACGGC 3 cut(s) 1575, 1948, 3024
BciT130I CCWGG 5 cut(s) 2127, 2686, 3187, 3342, 3612
BciVI GTATCC 1 cut(s) 3377
BclI TGATCA 3 cut(s) 2338, 2749, 2857
BcnI CCSGG 2 cut(s) 287, 3567
BcoDI GTCTC 7 cut(s) 138, 310, 803, 1388, 2803, 3377, 3455
BcuI ACTAGT 1 cut(s) 3208
BfmI CTRYAG 5 cut(s) 616, 962, 1627, 1889, 2186
BfrI CTTAAG 2 cut(s) 76, 1322
BfuI GTATCC 1 cut(s) 3377
BglI GCCNNNNNGGC 2 cut(s) 1932, 3550
BglII AGATCT 3 cut(s) 446, 881, 1423
BlnI CCTAGG 2 cut(s) 901, 3136
BmcAI AGTACT 1 cut(s) 3213
Bme1390I CCNGG 7 cut(s) 287, 2127, 2686, 3187, 3342, 3567, 3612
Bme18I GGWCC 7 cut(s) 1854, 2410, 2621, 2910, 3022, 3426, 3584
BmeT110I CYCGRG 3 cut(s) 782, 1480, 2888
BmgBI CACGTC 2 cut(s) 2353, 2795
BmgT120I GGNCC 9 cut(s) 1854, 2410, 2495, 2621, 2910, 3022, 3426, 3584, 3598
BmiI GGNNCC 9 cut(s) 303, 623, 1314, 1634, 1723, 2623, 2690, 2911, 3038
BmrFI CCNGG 7 cut(s) 287, 2127, 2686, 3187, 3342, 3567, 3612
BmrI ACTGGG 1 cut(s) 2351
BmuI ACTGGG 1 cut(s) 2351
BpiI GAAGAC 3 cut(s) 626, 2379, 3072
BplI GAGNNNNNCTC 6 cut(s) 412, 444, 1352, 1384, 2122, 2154
BpmI CTGGAG 2 cut(s) 359, 437
Bpu14I TTCGAA 1 cut(s) 454
BpuEI CTTGAG 5 cut(s) 332, 679, 785, 2594, 3367
BpuMI CCSGG 2 cut(s) 287, 3567
BsaAI YACGTR 2 cut(s) 1115, 1694
BsaBI GATNNNNATC 1 cut(s) 2337
BsaHI GRCGYC 1 cut(s) 1381
BsaI GGTCTC 1 cut(s) 803
BsaWI WCCGGW 1 cut(s) 2288
BsaXI ACNNNNNCTCC 2 cut(s) 3099, 3129
Bse1I ACTGG 7 cut(s) 387, 1649, 1856, 2357, 2363, 3468, 3587
Bse3DI GCAATG 2 cut(s) 2311, 3154
Bse8I GATNNNNATC 1 cut(s) 2337
BseAI TCCGGA 1 cut(s) 2288
BseBI CCWGG 5 cut(s) 2127, 2686, 3187, 3342, 3612
BseJI GATNNNNATC 1 cut(s) 2337
BseMI GCAATG 2 cut(s) 2311, 3154
BseNI ACTGG 7 cut(s) 387, 1649, 1856, 2357, 2363, 3468, 3587
BseRI GAGGAG 4 cut(s) 758, 1168, 1220, 1754
BseXI GCAGC 8 cut(s) 549, 602, 1613, 1673, 2029, 2098, 3190, 3243
BsgI GTGCAG 1 cut(s) 3189
Bsh1285I CGRYCG 2 cut(s) 1070, 2002
BshFI GGCC 4 cut(s) 591, 2496, 3141, 3599
BsiEI CGRYCG 2 cut(s) 1070, 2002
BsiHKAI GWGCWC 2 cut(s) 695, 3057
BsiHKCI CYCGRG 3 cut(s) 782, 1480, 2888
BsiSI CCGG 3 cut(s) 287, 2289, 3567
BslFI GGGAC 4 cut(s) 1570, 2339, 2556, 2923
BsmAI GTCTC 7 cut(s) 138, 310, 803, 1388, 2803, 3377, 3455
BsmBI CGTCTC 3 cut(s) 1388, 3377, 3455
BsmFI GGGAC 4 cut(s) 1570, 2339, 2556, 2923
BsmI GAATGC 1 cut(s) 2946
BsnI GGCC 4 cut(s) 591, 2496, 3141, 3599
Bso31I GGTCTC 1 cut(s) 803
BsoBI CYCGRG 3 cut(s) 782, 1480, 2888
Bsp119I TTCGAA 1 cut(s) 454
Bsp1286I GDGCHC 2 cut(s) 695, 3057
Bsp13I TCCGGA 1 cut(s) 2288
Bsp19I CCATGG 3 cut(s) 826, 1725, 3494
BspACI CCGC 6 cut(s) 199, 612, 1163, 2164, 3118, 3253
BspANI GGCC 4 cut(s) 591, 2496, 3141, 3599
BspEI TCCGGA 1 cut(s) 2288
BspHI TCATGA 4 cut(s) 2115, 2698, 2746, 2860
BspLI GGNNCC 9 cut(s) 303, 623, 1314, 1634, 1723, 2623, 2690, 2911, 3038
BspMAI CTGCAG 3 cut(s) 620, 966, 1631
BspPI GGATC 6 cut(s) 261, 316, 378, 1089, 1288, 1965
BspT104I TTCGAA 1 cut(s) 454
BspTI CTTAAG 2 cut(s) 76, 1322
BspTNI GGTCTC 1 cut(s) 803
BsrDI GCAATG 2 cut(s) 2311, 3154
BsrI ACTGG 7 cut(s) 387, 1649, 1856, 2357, 2363, 3468, 3587
BssNAI GTATAC 2 cut(s) 2054, 2123
BssNI GRCGYC 1 cut(s) 1381
BssT1I CCWWGG 5 cut(s) 826, 901, 1725, 3136, 3494
Bst1107I GTATAC 2 cut(s) 2054, 2123
Bst2UI CCWGG 5 cut(s) 2127, 2686, 3187, 3342, 3612
Bst6I CTCTTC 3 cut(s) 456, 2633, 3384
BstACI GRCGYC 1 cut(s) 1381
BstAFI CTTAAG 2 cut(s) 76, 1322
BstAPI GCANNNNNTGC 1 cut(s) 2023
BstBAI YACGTR 2 cut(s) 1115, 1694
BstBI TTCGAA 1 cut(s) 454
BstC8I GCNNGC 4 cut(s) 83, 3097, 3101, 3143
BstDSI CCRYGG 3 cut(s) 826, 1725, 3494
BstHHI GCGC 2 cut(s) 2990, 3147
BstMAI GTCTC 7 cut(s) 138, 310, 803, 1388, 2803, 3377, 3455
BstMCI CGRYCG 2 cut(s) 1070, 2002
BstNI CCWGG 5 cut(s) 2127, 2686, 3187, 3342, 3612
BstNSI RCATGY 4 cut(s) 2228, 2939, 3103, 3116
BstSCI CCNGG 7 cut(s) 285, 2125, 2684, 3185, 3340, 3565, 3610
BstSFI CTRYAG 5 cut(s) 616, 962, 1627, 1889, 2186
BstV1I GCAGC 8 cut(s) 549, 602, 1613, 1673, 2029, 2098, 3190, 3243
BstV2I GAAGAC 3 cut(s) 626, 2379, 3072
BstX2I RGATCY 8 cut(s) 253, 370, 446, 881, 1081, 1293, 1423, 1957
BstXI CCANNNNNNTGG 1 cut(s) 3032
BstYI RGATCY 8 cut(s) 253, 370, 446, 881, 1081, 1293, 1423, 1957
BstZ17I GTATAC 2 cut(s) 2054, 2123
BsuI GTATCC 1 cut(s) 3377
BsuRI GGCC 4 cut(s) 591, 2496, 3141, 3599
BtgI CCRYGG 3 cut(s) 826, 1725, 3494
BtrI CACGTC 2 cut(s) 2353, 2795
BtsI GCAGTG 4 cut(s) 959, 2497, 2824, 3159
BtsIMutI CAGTG 7 cut(s) 665, 959, 1969, 2497, 2536, 2824, 3159
Cac8I GCNNGC 4 cut(s) 83, 3097, 3101, 3143
CaiI CAGNNNCTG 3 cut(s) 382, 970, 2369
CciI TCATGA 4 cut(s) 2115, 2698, 2746, 2860
CfoI GCGC 2 cut(s) 2990, 3147
Cfr13I GGNCC 9 cut(s) 1854, 2410, 2495, 2621, 2910, 3022, 3426, 3584, 3598
CseI GACGC 2 cut(s) 1370, 3437
CsiI ACCWGGT 1 cut(s) 2684
Csp6I GTAC 4 cut(s) 336, 2457, 2939, 3212
CspCI CAANNNNNGTGG 2 cut(s) 2946, 2981
CviQI GTAC 4 cut(s) 336, 2457, 2939, 3212
DraI TTTAAA 1 cut(s) 160
Eam1104I CTCTTC 3 cut(s) 456, 2633, 3384
EarI CTCTTC 3 cut(s) 456, 2633, 3384
EciI GGCGGA 2 cut(s) 1152, 2153
Eco130I CCWWGG 5 cut(s) 826, 901, 1725, 3136, 3494
Eco31I GGTCTC 1 cut(s) 803
Eco32I GATATC 2 cut(s) 663, 1160
Eco47I GGWCC 7 cut(s) 1854, 2410, 2621, 2910, 3022, 3426, 3584
Eco57I CTGAAG 2 cut(s) 277, 2391
Eco88I CYCGRG 3 cut(s) 782, 1480, 2888
EcoO109I RGGNCCY 1 cut(s) 2910
EcoRI GAATTC 2 cut(s) 179, 2463
EcoRII CCWGG 5 cut(s) 2125, 2684, 3185, 3340, 3610
EcoRV GATATC 2 cut(s) 663, 1160
EcoT14I CCWWGG 5 cut(s) 826, 901, 1725, 3136, 3494
EcoT22I ATGCAT 3 cut(s) 523, 2313, 2880
ErhI CCWWGG 5 cut(s) 826, 901, 1725, 3136, 3494
Esp3I CGTCTC 3 cut(s) 1388, 3377, 3455
FaqI GGGAC 4 cut(s) 1570, 2339, 2556, 2923
FbaI TGATCA 3 cut(s) 2338, 2749, 2857
FblI GTMKAC 3 cut(s) 1860, 2053, 2122
GlaI GCGC 2 cut(s) 2989, 3146
GsuI CTGGAG 2 cut(s) 359, 437
HaeIII GGCC 4 cut(s) 591, 2496, 3141, 3599
HapII CCGG 3 cut(s) 287, 2289, 3567
HgaI GACGC 2 cut(s) 1370, 3437
HhaI GCGC 2 cut(s) 2990, 3147
Hin1I GRCGYC 1 cut(s) 1381
Hin6I GCGC 2 cut(s) 2988, 3145
HinP1I GCGC 2 cut(s) 2988, 3145
HincII GTYRAC 3 cut(s) 1861, 3007, 3461
HindII GTYRAC 3 cut(s) 1861, 3007, 3461
HindIII AAGCTT 2 cut(s) 1802, 2150
HinfI GANTC 9 cut(s) 140, 394, 631, 785, 805, 967, 1363, 2479, 2968
HpaI GTTAAC 1 cut(s) 3007
HpaII CCGG 3 cut(s) 287, 2289, 3567
HphI GGTGA 5 cut(s) 347, 959, 988, 2081, 2645
Hpy166II GTNNAC 7 cut(s) 355, 1861, 2054, 2123, 3007, 3355, 3461
Hpy8I GTNNAC 7 cut(s) 355, 1861, 2054, 2123, 3007, 3355, 3461
Hpy99I CGWCG 2 cut(s) 1862, 2796
HpyCH4IV ACGT 6 cut(s) 30, 1114, 1693, 2352, 2794, 2838
HpySE526I ACGT 6 cut(s) 30, 1114, 1693, 2352, 2794, 2838
Hsp92I GRCGYC 1 cut(s) 1381
HspAI GCGC 2 cut(s) 2988, 3145
Kpn2I TCCGGA 1 cut(s) 2288
Ksp22I TGATCA 3 cut(s) 2338, 2749, 2857
KspAI GTTAAC 1 cut(s) 3007
Lsp1109I GCAGC 8 cut(s) 549, 602, 1613, 1673, 2029, 2098, 3190, 3243
MabI ACCWGGT 1 cut(s) 2684
MaeII ACGT 6 cut(s) 30, 1114, 1693, 2352, 2794, 2838
MaeIII GTNAC 7 cut(s) 2069, 2102, 2503, 2525, 2713, 2723, 3412
MfeI CAATTG 4 cut(s) 363, 1374, 1592, 3175
MflI RGATCY 8 cut(s) 253, 370, 446, 881, 1081, 1293, 1423, 1957
MhlI GDGCHC 2 cut(s) 695, 3057
MlyI GAGTC 4 cut(s) 640, 794, 976, 2488
MmeI TCCRAC 6 cut(s) 285, 720, 1130, 1411, 2705, 2761
Mph1103I ATGCAT 3 cut(s) 523, 2313, 2880
MroI TCCGGA 1 cut(s) 2288
MroXI GAANNNNTTC 2 cut(s) 866, 3486
MslI CAYNNNNRTG 8 cut(s) 585, 1551, 2031, 2229, 2239, 2652, 2817, 2865
MspCI CTTAAG 2 cut(s) 76, 1322
MspI CCGG 3 cut(s) 287, 2289, 3567
MspR9I CCNGG 7 cut(s) 287, 2127, 2686, 3187, 3342, 3567, 3612
MunI CAATTG 4 cut(s) 363, 1374, 1592, 3175
Mva1269I GAATGC 1 cut(s) 2946
MvaI CCWGG 5 cut(s) 2127, 2686, 3187, 3342, 3612
NciI CCSGG 2 cut(s) 287, 3567
NcoI CCATGG 3 cut(s) 826, 1725, 3494
NlaIV GGNNCC 9 cut(s) 303, 623, 1314, 1634, 1723, 2623, 2690, 2911, 3038
NmeAIII GCCGAG 1 cut(s) 527
NmuCI GTSAC 4 cut(s) 2069, 2102, 2503, 2723
NsiI ATGCAT 3 cut(s) 523, 2313, 2880
NspI RCATGY 4 cut(s) 2228, 2939, 3103, 3116
NspV TTCGAA 1 cut(s) 454
PaeI GCATGC 1 cut(s) 3103
PaeR7I CTCGAG 1 cut(s) 2888
PagI TCATGA 4 cut(s) 2115, 2698, 2746, 2860
PciI ACATGT 3 cut(s) 2224, 2935, 3112
PctI GAATGC 1 cut(s) 2946
PdmI GAANNNNTTC 2 cut(s) 866, 3486
PfeI GAWTC 5 cut(s) 140, 394, 805, 1363, 2968
PflMI CCANNNNNTGG 4 cut(s) 365, 382, 583, 2475
PleI GAGTC 4 cut(s) 639, 793, 975, 2487
PpsI GAGTC 4 cut(s) 639, 793, 975, 2487
Ppu21I YACGTR 2 cut(s) 1115, 1694
PpuMI RGGWCCY 1 cut(s) 2910
PscI ACATGT 3 cut(s) 2224, 2935, 3112
PshBI ATTAAT 1 cut(s) 2895
Psp1406I AACGTT 1 cut(s) 2838
Psp5II RGGWCCY 1 cut(s) 2910
Psp6I CCWGG 5 cut(s) 2125, 2684, 3185, 3340, 3610
PspGI CCWGG 5 cut(s) 2125, 2684, 3185, 3340, 3610
PspN4I GGNNCC 9 cut(s) 303, 623, 1314, 1634, 1723, 2623, 2690, 2911, 3038
PspPI GGNCC 9 cut(s) 1854, 2410, 2495, 2621, 2910, 3022, 3426, 3584, 3598
PspPPI RGGWCCY 1 cut(s) 2910
PstI CTGCAG 3 cut(s) 620, 966, 1631
PstNI CAGNNNCTG 3 cut(s) 382, 970, 2369
PsuI RGATCY 8 cut(s) 253, 370, 446, 881, 1081, 1293, 1423, 1957
RsaI GTAC 4 cut(s) 337, 2458, 2940, 3213
RsaNI GTAC 4 cut(s) 336, 2457, 2939, 3212
RseI CAYNNNNRTG 8 cut(s) 585, 1551, 2031, 2229, 2239, 2652, 2817, 2865
SalI GTCGAC 1 cut(s) 1859
Sau96I GGNCC 9 cut(s) 1854, 2410, 2495, 2621, 2910, 3022, 3426, 3584, 3598
ScaI AGTACT 1 cut(s) 3213
SchI GAGTC 4 cut(s) 640, 794, 976, 2488
ScrFI CCNGG 7 cut(s) 287, 2127, 2686, 3187, 3342, 3567, 3612
SduI GDGCHC 2 cut(s) 695, 3057
SexAI ACCWGGT 1 cut(s) 2684
SfcI CTRYAG 5 cut(s) 616, 962, 1627, 1889, 2186
Sfr274I CTCGAG 1 cut(s) 2888
SfuI TTCGAA 1 cut(s) 454
SinI GGWCC 7 cut(s) 1854, 2410, 2621, 2910, 3022, 3426, 3584
SlaI CTCGAG 1 cut(s) 2888
SmiMI CAYNNNNRTG 8 cut(s) 585, 1551, 2031, 2229, 2239, 2652, 2817, 2865
SmlI CTYRAG 8 cut(s) 76, 311, 694, 800, 1322, 2573, 2888, 3346
SmoI CTYRAG 8 cut(s) 76, 311, 694, 800, 1322, 2573, 2888, 3346
SpeI ACTAGT 1 cut(s) 3208
SphI GCATGC 1 cut(s) 3103
SsiI CCGC 6 cut(s) 199, 612, 1163, 2164, 3118, 3253
SspI AATATT 2 cut(s) 272, 1975
StyD4I CCNGG 7 cut(s) 285, 2125, 2684, 3185, 3340, 3565, 3610
StyI CCWWGG 5 cut(s) 826, 901, 1725, 3136, 3494
TaiI ACGT 6 cut(s) 33, 1117, 1696, 2355, 2797, 2841
TaqII GACCGA 2 cut(s) 1988, 2427
TatI WGTACW 2 cut(s) 335, 3211
TauI GCSGC 3 cut(s) 201, 615, 3256
TfiI GAWTC 5 cut(s) 140, 394, 805, 1363, 2968
TscAI CASTG 7 cut(s) 672, 966, 1969, 2497, 2536, 2824, 3159
TseFI GTSAC 4 cut(s) 2069, 2102, 2503, 2723
TseI GCWGC 8 cut(s) 562, 615, 1626, 1686, 2017, 2086, 3203, 3256
Tsp45I GTSAC 4 cut(s) 2069, 2102, 2503, 2723
TspGWI ACGGA 4 cut(s) 1077, 1846, 3359, 3431
TspRI CASTG 7 cut(s) 672, 966, 1969, 2497, 2536, 2824, 3159
Van91I CCANNNNNTGG 4 cut(s) 365, 382, 583, 2475
Vha464I CTTAAG 2 cut(s) 76, 1322
VpaK11BI GGWCC 7 cut(s) 1854, 2410, 2621, 2910, 3022, 3426, 3584
VspI ATTAAT 1 cut(s) 2895
XbaI TCTAGA 3 cut(s) 136, 808, 1426
XceI RCATGY 4 cut(s) 2228, 2939, 3103, 3116
XcmI CCANNNNNNNNNTGG 3 cut(s) 584, 833, 1732
XhoI CTCGAG 1 cut(s) 2888
XmaJI CCTAGG 2 cut(s) 901, 3136
XmiI GTMKAC 3 cut(s) 1860, 2053, 2122
XmnI GAANNNNTTC 2 cut(s) 866, 3486
ZrmI AGTACT 1 cut(s) 3213
Zsp2I ATGCAT 3 cut(s) 523, 2313, 2880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.