resistance protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 834, 1162 |
| Acc16I | TGCGCA | 1 cut(s) | 279 |
| AccII | CGCG | 1 cut(s) | 87 |
| AccIII | TCCGGA | 1 cut(s) | 473 |
| AciI | CCGC | 1 cut(s) | 85 |
| AclWI | GGATC | 2 cut(s) | 335, 620 |
| AcoI | YGGCCR | 1 cut(s) | 799 |
| AcsI | RAATTY | 3 cut(s) | 315, 373, 746 |
| AfaI | GTAC | 3 cut(s) | 48, 110, 815 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 90 |
| AgsI | TTSAA | 7 cut(s) | 121, 196, 255, 314, 412, 719, 959 |
| AjnI | CCWGG | 4 cut(s) | 257, 936, 997, 1093 |
| AluBI | AGCT | 7 cut(s) | 274, 449, 530, 713, 895, 1103, 1111 |
| AluI | AGCT | 7 cut(s) | 274, 449, 530, 713, 895, 1103, 1111 |
| Alw21I | GWGCWC | 1 cut(s) | 930 |
| Alw26I | GTCTC | 1 cut(s) | 1020 |
| AlwI | GGATC | 2 cut(s) | 335, 620 |
| Aor13HI | TCCGGA | 1 cut(s) | 473 |
| AoxI | GGCC | 2 cut(s) | 384, 799 |
| ApoI | RAATTY | 3 cut(s) | 315, 373, 746 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 516, 589, 1104 |
| AspLEI | GCGC | 2 cut(s) | 280, 634 |
| AspS9I | GGNCC | 2 cut(s) | 385, 995 |
| AvaII | GGWCC | 1 cut(s) | 995 |
| BbsI | GAAGAC | 2 cut(s) | 49, 306 |
| Bbv12I | GWGCWC | 1 cut(s) | 930 |
| BccI | CCATC | 3 cut(s) | 83, 352, 471 |
| BciT130I | CCWGG | 4 cut(s) | 259, 938, 999, 1095 |
| BclI | TGATCA | 1 cut(s) | 862 |
| BcoDI | GTCTC | 1 cut(s) | 1020 |
| BfaI | CTAG | 1 cut(s) | 809 |
| BfoI | RGCGCY | 1 cut(s) | 635 |
| BglII | AGATCT | 1 cut(s) | 544 |
| BlpI | GCTNAGC | 1 cut(s) | 393 |
| Bme1390I | CCNGG | 4 cut(s) | 259, 938, 999, 1095 |
| Bme18I | GGWCC | 1 cut(s) | 995 |
| BmgT120I | GGNCC | 2 cut(s) | 385, 995 |
| BmiI | GGNNCC | 2 cut(s) | 7, 996 |
| BmrFI | CCNGG | 4 cut(s) | 259, 938, 999, 1095 |
| BmsI | GCATC | 3 cut(s) | 32, 600, 749 |
| BpiI | GAAGAC | 2 cut(s) | 49, 306 |
| BpmI | CTGGAG | 2 cut(s) | 1116, 1131 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 393 |
| BpuEI | CTTGAG | 1 cut(s) | 182 |
| Bsa29I | ATCGAT | 1 cut(s) | 1153 |
| BsaAI | YACGTR | 1 cut(s) | 563 |
| BsaJI | CCNNGG | 1 cut(s) | 802 |
| BsaWI | WCCGGW | 1 cut(s) | 473 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 90 |
| Bse1I | ACTGG | 1 cut(s) | 323 |
| Bse3DI | GCAATG | 1 cut(s) | 301 |
| BseAI | TCCGGA | 1 cut(s) | 473 |
| BseBI | CCWGG | 4 cut(s) | 259, 938, 999, 1095 |
| BseCI | ATCGAT | 1 cut(s) | 1153 |
| BseDI | CCNNGG | 1 cut(s) | 802 |
| BseGI | GGATG | 2 cut(s) | 154, 482 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 90 |
| BseMI | GCAATG | 1 cut(s) | 301 |
| BseMII | CTCAG | 2 cut(s) | 53, 339 |
| BseNI | ACTGG | 1 cut(s) | 323 |
| Bsh1236I | CGCG | 1 cut(s) | 87 |
| BshFI | GGCC | 2 cut(s) | 386, 801 |
| BshVI | ATCGAT | 1 cut(s) | 1153 |
| BsiHKAI | GWGCWC | 1 cut(s) | 930 |
| BsiSI | CCGG | 2 cut(s) | 474, 845 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 90 |
| BsmAI | GTCTC | 1 cut(s) | 1020 |
| BsnI | GGCC | 2 cut(s) | 386, 801 |
| Bsp1286I | GDGCHC | 1 cut(s) | 930 |
| Bsp13I | TCCGGA | 1 cut(s) | 473 |
| Bsp1407I | TGTACA | 1 cut(s) | 108 |
| Bsp143I | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 393 |
| BspACI | CCGC | 1 cut(s) | 85 |
| BspANI | GGCC | 2 cut(s) | 386, 801 |
| BspCNI | CTCAG | 2 cut(s) | 54, 340 |
| BspDI | ATCGAT | 1 cut(s) | 1153 |
| BspEI | TCCGGA | 1 cut(s) | 473 |
| BspFNI | CGCG | 1 cut(s) | 87 |
| BspLI | GGNNCC | 2 cut(s) | 7, 996 |
| BspPI | GGATC | 2 cut(s) | 335, 620 |
| BspQI | GCTCTTC | 1 cut(s) | 1118 |
| BsrDI | GCAATG | 1 cut(s) | 301 |
| BsrGI | TGTACA | 1 cut(s) | 108 |
| BsrI | ACTGG | 1 cut(s) | 323 |
| BssECI | CCNNGG | 1 cut(s) | 802 |
| BssMI | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| Bst2UI | CCWGG | 4 cut(s) | 259, 938, 999, 1095 |
| Bst4CI | ACNGT | 2 cut(s) | 558, 773 |
| Bst6I | CTCTTC | 1 cut(s) | 1118 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 29 |
| BstAUI | TGTACA | 1 cut(s) | 108 |
| BstBAI | YACGTR | 1 cut(s) | 563 |
| BstC8I | GCNNGC | 1 cut(s) | 276 |
| BstDEI | CTNAG | 3 cut(s) | 62, 348, 393 |
| BstF5I | GGATG | 2 cut(s) | 154, 482 |
| BstFNI | CGCG | 1 cut(s) | 87 |
| BstH2I | RGCGCY | 1 cut(s) | 635 |
| BstHHI | GCGC | 2 cut(s) | 280, 634 |
| BstKTI | GATC | 7 cut(s) | 343, 547, 628, 865, 966, 1153, 1157 |
| BstMAI | GTCTC | 1 cut(s) | 1020 |
| BstMBI | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 29, 710 |
| BstNI | CCWGG | 4 cut(s) | 259, 938, 999, 1095 |
| BstNSI | RCATGY | 2 cut(s) | 23, 461 |
| BstSCI | CCNGG | 4 cut(s) | 257, 936, 997, 1093 |
| BstUI | CGCG | 1 cut(s) | 87 |
| BstV2I | GAAGAC | 2 cut(s) | 49, 306 |
| BstX2I | RGATCY | 1 cut(s) | 544 |
| BstYI | RGATCY | 1 cut(s) | 544 |
| Bsu15I | ATCGAT | 1 cut(s) | 1153 |
| BsuRI | GGCC | 2 cut(s) | 386, 801 |
| BsuTUI | ATCGAT | 1 cut(s) | 1153 |
| BtgZI | GCGATG | 1 cut(s) | 102 |
| BtsCI | GGATG | 2 cut(s) | 154, 482 |
| BtsI | GCAGTG | 1 cut(s) | 111 |
| BtsIMutI | CAGTG | 1 cut(s) | 111 |
| Cac8I | GCNNGC | 1 cut(s) | 276 |
| CfoI | GCGC | 2 cut(s) | 280, 634 |
| Cfr13I | GGNCC | 2 cut(s) | 385, 995 |
| ClaI | ATCGAT | 1 cut(s) | 1153 |
| CsiI | ACCWGGT | 1 cut(s) | 257 |
| Csp6I | GTAC | 3 cut(s) | 47, 109, 814 |
| CviAII | CATG | 6 cut(s) | 20, 50, 398, 458, 797, 1142 |
| CviQI | GTAC | 3 cut(s) | 47, 109, 814 |
| DdeI | CTNAG | 3 cut(s) | 62, 348, 393 |
| DpnI | GATC | 7 cut(s) | 342, 546, 627, 864, 965, 1152, 1156 |
| DpnII | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| DraI | TTTAAA | 2 cut(s) | 453, 751 |
| EaeI | YGGCCR | 1 cut(s) | 799 |
| Eam1104I | CTCTTC | 1 cut(s) | 1118 |
| EarI | CTCTTC | 1 cut(s) | 1118 |
| Eco47I | GGWCC | 1 cut(s) | 995 |
| EcoO109I | RGGNCCY | 1 cut(s) | 385 |
| EcoRII | CCWGG | 4 cut(s) | 257, 936, 997, 1093 |
| EcoT22I | ATGCAT | 1 cut(s) | 25 |
| FaeI | CATG | 6 cut(s) | 23, 53, 401, 461, 800, 1145 |
| FatI | CATG | 6 cut(s) | 19, 49, 397, 457, 796, 1141 |
| FauI | CCCGC | 1 cut(s) | 92 |
| FbaI | TGATCA | 1 cut(s) | 862 |
| FokI | GGATG | 2 cut(s) | 161, 489 |
| FspBI | CTAG | 1 cut(s) | 809 |
| FspI | TGCGCA | 1 cut(s) | 279 |
| GlaI | GCGC | 2 cut(s) | 279, 633 |
| GsuI | CTGGAG | 2 cut(s) | 1116, 1131 |
| HaeII | RGCGCY | 1 cut(s) | 635 |
| HaeIII | GGCC | 2 cut(s) | 386, 801 |
| HapII | CCGG | 2 cut(s) | 474, 845 |
| HhaI | GCGC | 2 cut(s) | 280, 634 |
| Hin1II | CATG | 6 cut(s) | 23, 53, 401, 461, 800, 1145 |
| Hin6I | GCGC | 2 cut(s) | 278, 632 |
| HinP1I | GCGC | 2 cut(s) | 278, 632 |
| HindIII | AAGCTT | 3 cut(s) | 447, 711, 1101 |
| HinfI | GANTC | 8 cut(s) | 132, 209, 506, 590, 838, 909, 1008, 1090 |
| HpaII | CCGG | 2 cut(s) | 474, 845 |
| Hpy166II | GTNNAC | 3 cut(s) | 96, 111, 814 |
| Hpy188I | TCNGA | 6 cut(s) | 238, 349, 489, 511, 867, 1122 |
| Hpy8I | GTNNAC | 3 cut(s) | 96, 111, 814 |
| Hpy99I | CGWCG | 1 cut(s) | 1039 |
| HpyAV | CCTTC | 2 cut(s) | 190, 421 |
| HpyCH4III | ACNGT | 2 cut(s) | 558, 773 |
| HpyCH4IV | ACGT | 3 cut(s) | 336, 562, 1037 |
| HpyCH4V | TGCA | 6 cut(s) | 23, 32, 294, 613, 820, 905 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 29, 710 |
| HpyF3I | CTNAG | 3 cut(s) | 62, 348, 393 |
| HpySE526I | ACGT | 3 cut(s) | 336, 562, 1037 |
| Hsp92II | CATG | 6 cut(s) | 23, 53, 401, 461, 800, 1145 |
| HspAI | GCGC | 2 cut(s) | 278, 632 |
| Kpn2I | TCCGGA | 1 cut(s) | 473 |
| Ksp22I | TGATCA | 1 cut(s) | 862 |
| Kzo9I | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| LguI | GCTCTTC | 1 cut(s) | 1118 |
| LmnI | GCTCC | 1 cut(s) | 1150 |
| LweI | GCATC | 3 cut(s) | 32, 600, 749 |
| MabI | ACCWGGT | 1 cut(s) | 257 |
| MaeI | CTAG | 1 cut(s) | 809 |
| MaeII | ACGT | 3 cut(s) | 336, 562, 1037 |
| MaeIII | GTNAC | 2 cut(s) | 558, 1015 |
| MalI | GATC | 7 cut(s) | 342, 546, 627, 864, 965, 1152, 1156 |
| MboI | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| MboII | GAAGA | 6 cut(s) | 49, 243, 311, 413, 416, 1105 |
| MfeI | CAATTG | 1 cut(s) | 201 |
| MflI | RGATCY | 1 cut(s) | 544 |
| MhlI | GDGCHC | 1 cut(s) | 930 |
| MluCI | AATT | 6 cut(s) | 201, 315, 373, 513, 746, 823 |
| MlyI | GAGTC | 5 cut(s) | 515, 832, 903, 1017, 1084 |
| MmeI | TCCRAC | 1 cut(s) | 1100 |
| Mph1103I | ATGCAT | 1 cut(s) | 25 |
| MroI | TCCGGA | 1 cut(s) | 473 |
| MroXI | GAANNNNTTC | 3 cut(s) | 516, 589, 1104 |
| MseI | TTAA | 6 cut(s) | 180, 452, 750, 899, 1040, 1131 |
| MspI | CCGG | 2 cut(s) | 474, 845 |
| MspR9I | CCNGG | 4 cut(s) | 259, 938, 999, 1095 |
| MunI | CAATTG | 1 cut(s) | 201 |
| MvaI | CCWGG | 4 cut(s) | 259, 938, 999, 1095 |
| MvnI | CGCG | 1 cut(s) | 87 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 29, 710 |
| NdeII | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| NlaIII | CATG | 6 cut(s) | 23, 53, 401, 461, 800, 1145 |
| NlaIV | GGNNCC | 2 cut(s) | 7, 996 |
| NmeAIII | GCCGAG | 2 cut(s) | 574, 827 |
| NsbI | TGCGCA | 1 cut(s) | 279 |
| NsiI | ATGCAT | 1 cut(s) | 25 |
| NspI | RCATGY | 2 cut(s) | 23, 461 |
| PciSI | GCTCTTC | 1 cut(s) | 1118 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 939 |
| PdmI | GAANNNNTTC | 3 cut(s) | 516, 589, 1104 |
| PfeI | GAWTC | 3 cut(s) | 132, 209, 590 |
| PfoI | TCCNGGA | 3 cut(s) | 936, 997, 1093 |
| PleI | GAGTC | 5 cut(s) | 514, 832, 903, 1016, 1084 |
| PpsI | GAGTC | 5 cut(s) | 514, 832, 903, 1016, 1084 |
| Ppu21I | YACGTR | 1 cut(s) | 563 |
| PsiI | TTATAA | 2 cut(s) | 834, 1162 |
| Psp6I | CCWGG | 4 cut(s) | 257, 936, 997, 1093 |
| PspGI | CCWGG | 4 cut(s) | 257, 936, 997, 1093 |
| PspN4I | GGNNCC | 2 cut(s) | 7, 996 |
| PspPI | GGNCC | 2 cut(s) | 385, 995 |
| PsuI | RGATCY | 1 cut(s) | 544 |
| RsaI | GTAC | 3 cut(s) | 48, 110, 815 |
| RsaNI | GTAC | 3 cut(s) | 47, 109, 814 |
| SapI | GCTCTTC | 1 cut(s) | 1118 |
| SaqAI | TTAA | 6 cut(s) | 180, 452, 750, 899, 1040, 1131 |
| Sau3AI | GATC | 7 cut(s) | 340, 544, 625, 862, 963, 1150, 1154 |
| Sau96I | GGNCC | 2 cut(s) | 385, 995 |
| SchI | GAGTC | 5 cut(s) | 515, 832, 903, 1017, 1084 |
| ScrFI | CCNGG | 4 cut(s) | 259, 938, 999, 1095 |
| SduI | GDGCHC | 1 cut(s) | 930 |
| SexAI | ACCWGGT | 1 cut(s) | 257 |
| SfaNI | GCATC | 3 cut(s) | 32, 600, 749 |
| SinI | GGWCC | 1 cut(s) | 995 |
| SmlI | CTYRAG | 1 cut(s) | 161 |
| SmoI | CTYRAG | 1 cut(s) | 161 |
| Sse9I | AATT | 6 cut(s) | 201, 315, 373, 513, 746, 823 |
| SsiI | CCGC | 1 cut(s) | 85 |
| SspI | AATATT | 1 cut(s) | 104 |
| SspMI | CTAG | 1 cut(s) | 809 |
| StyD4I | CCNGG | 4 cut(s) | 257, 936, 997, 1093 |
| TaaI | ACNGT | 2 cut(s) | 558, 773 |
| TaiI | ACGT | 3 cut(s) | 339, 565, 1040 |
| TaqI | TCGA | 4 cut(s) | 212, 464, 1006, 1153 |
| TasI | AATT | 6 cut(s) | 201, 315, 373, 513, 746, 823 |
| TatI | WGTACW | 2 cut(s) | 108, 813 |
| TfiI | GAWTC | 3 cut(s) | 132, 209, 590 |
| Tru1I | TTAA | 6 cut(s) | 180, 452, 750, 899, 1040, 1131 |
| Tru9I | TTAA | 6 cut(s) | 180, 452, 750, 899, 1040, 1131 |
| TscAI | CASTG | 1 cut(s) | 118 |
| TspDTI | ATGAA | 2 cut(s) | 312, 414 |
| TspGWI | ACGGA | 1 cut(s) | 902 |
| TspRI | CASTG | 1 cut(s) | 118 |
| VpaK11BI | GGWCC | 1 cut(s) | 995 |
| XapI | RAATTY | 3 cut(s) | 315, 373, 746 |
| XceI | RCATGY | 2 cut(s) | 23, 461 |
| XmnI | GAANNNNTTC | 3 cut(s) | 516, 589, 1104 |
| XspI | CTAG | 1 cut(s) | 809 |
| Zsp2I | ATGCAT | 1 cut(s) | 25 |