Rh2BG534300

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000307
Physical Location & Seq
Reverse (-)
74303373 .. 74309393
6021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG534300.1

Sequence Viewer

Length: 3381 bp
ATGAATTCTCGTATAGAGAAAGTGGAGTCCTACTTGGATCTAAGCCTGGATGTTGTTCGCACAATTGGGATTTCGGGAATGGGAGGCATCGGTAAAACAACTCTTGCACAAGAAGTTTTTAAGAAGATCCTCAAAAACTTTCATACTAGCGCATTTGTCGCTAATGTTGGAGAGAAATCAGGAAATGTAAATGGTCTAGTTGACTTGCAAAAATTCCTTTATAAATCCTTGCTACACAGAGATGCAGATATAGATACAGATGACATGGGAAAATATTTGTTAAGGAAAGGACTACGTTCAAAGAAGGTGCTCATCATTCTAGACGATGTTCATGAACTCAAACAAGTGGAAGCTCTTGCTGGAAGTAGTAAACAGAATCCTTGGTTTGGTCCAGGGAGTAGAGTAATTATAACAACTAGAGATGAGCAGTTGTTGAAGTCTTATGACGTGCACAAGACATACGAGGTTGAGAAACTGACTGATGCTGAAGCTTCTCAGCTCTTGTGTCAGAAAGCCTTCAGGAAAAACCATGCTCCAACAGAATATCTAAAGCTGTCCAACAATTTTGTAAAATATGCCAGTGGCCTTCCTTTAGCACTTGAAGTTCTGGGTAAATACTTGTGTGGGAAGGAGGCAAATGAATGGTCAGCAGCATTGGCAAGATTATACGCAGATCCTGAAAAAGACATTTTGAGTATGCTTCAATTAAGTTATAATGGATTGAATAAGTCAACGGTGAAGGACATGTTTCTGGACATTGCATGTTTTTTCCGAGGAGAGAACCAAGATCGTGTAATAAAGATCTTCACGTGTTGTGACTTTCATCCTGAAATCGGCATTGTTGACCTTATTGATAAATCTTTGATTAAAATTGAAGGGAAAAAACTGTGGATGCATGATTTACTACAACAAATGGGTTGGCAGATTGTACGTCAAGAATCTCCTAAAGAGCCGGGTAAACGTAGTAGGTTGTGGCTTAATGAGAATGCTTGTGAGTACGAACGCTTGAGGTCGTGGTTTTATGAGAAACAGTCGAGCAAATGCAGAAGGTTGTGGCTTTACAAGGATGCTCGTGCTGTACTCATGCATAATCTGGGAACAGAACATGTTGAAGGTTTATTCCTAAGCTTGGAGGCAAAAGAAGAGTTACGCTTGAATGCGGATCCGTTCTACAAAATGCCAAACCTAAGGTTGTTAAAGATTTATAATGCCAACTTTTCTGGAAGCATTGAACAAATTTCTCTCTCCCAAAATCTAAGGCTTTTGGAATGGCATGAATTTCCTTTGCATTCTTTGCCATCAAGTTTTAGACCAAATGTGCTTGTTGAACTCAAGATGCCAAACAGCCGTATTACACAACTATGGAGTGAAAAGCTTTCTGCGGAAATGCTGATACTTATGGATCTTTCAAACTGCCAGTACTTGACTAGGACCCCGGATTTCAGTACAGTTCCAAAGCTTGAAAGGTTGGTCCTCGAAGGTTGTAAGAGATTATCAGAGGTTCACCCAACAATTGGAGGTCTCCAACATCTGGTCATATTAAATTTGAAAGGTTGTGAAAGTTTAGAGAGCCTTCCTGACTCCATCAGCTTGGAGTCTCTTAAGACCTTAGTCCTTTCAGGATGTTCAAAAATCAAAAGGTTTCCAGAGATTATGGGAAACATGGAAAATATGTCGGAACTTTATCTAGATGGGACAGCTATACGGGAGCTGCCAATATCGTTGAAGCACTTAAGTGGCCTTAGTTTGTTAAATCTAAGAGGCTGCAAGAACCTCTCAAGTCTTCCAAGCTTCATTTGTAGTTTGACATCTCTGATATCTCTCAATCTATCAGGCTGTTCACTTATTGATCAACTGCCAGAAAACCTAGGGAGCTTGGAACATTTGCAAGAGCTTGATGCCTGTGAAACTTCCATAACAAAAGTTCCTTCGTCTATTTTAATGCTTCTGTTGAAGAACCTTAAACGATTATGTTTCCGTCGATGTAACGATTTATGGTTGCCAGCTCAGGACCCGATCTCGGTAGGTGTTTGGTCATTGACATCAAAATGTCTGAACAGTAGCAACAGTCCCATTAAACCACCATTGGTTATTGAAAGGCTCAAATCTTTTCCGAAGATTGTGGAAAACATGAAGGGTTTAGAAAAGGTGAACTACACGTCCTTAGGTGCCAAGATGGATATTTCCTCAAGTGCGAAGATGGATTCGTCCTCAAGTGCCAAGAAGGATGTAACTGGAATGGATTCTTGTGAAGAAAAAATGTGGATTTTGGACATGGGACGCATAGGAAAGACAACTATTTCACAAGAAGTTTTTGAAAAGATCTGTGACCTATTTGAAGATAGCTGTTTTGTTGGTCCTATTAGAGAGGAATCTGGAAAGCATGGTCTGGTAGTTAGATTCCAAGGTGATCTTTTCATATTCAAAATGATGGCCAAGGTTCCTTCGAAGCATTTAGGCTTATCCCATTTGACATCATTAAGTGTATCTGATGCCTATATAGATGATATCCCTGATGATATTGGCCTCATTTCATATTCACTTCAGTGTTTAGATTTGAGTGGAAATAATTATTTGTCGAGCATACCCGAAAGCATCTGTGAACTTTCTAAGCTCACAGAGCTTCGCTTGTACGGGTGTATTAGGCTTCGGTCATTACCAAAGTTTCTGCCGCTGAGTCTAAAATTTATAGATGCACGTTATTGTCCTATGCTGACAAATTTTCCAAATAATTGTGCAATATGGACTTCGGAGGAGGGTTTCAGTTTCATGGATTGCAGAAATTCAGTTAATGATATCGAAGATCATTTAAATATAATTCGTCTTAAATACATTGAGGATCGAATCTATCACGAACAGCCATTTGAAATCCATCTTCGTCACACTAGAATTCCAGACTGGTGTGGTTATTCTAGGAGAGGATCTTCTGTAACAATCCCACTATCTGATCCAGAAGATGGTACTAGTACGTGGATGGGATTTGTCCTTTTTGTTGTTTTTGAGATCCTTGACAATAGAAACTTTGATAAGAGTTGGGAATTTAAAGATACTTGTTGTCATTTTAGCACCTACGAAGATCCTCTTGAAAATCCCCTGGTTTTTCGATACTTCAGTAACCCCGGGGTTGGGCCATTTGTGCTCTGTTGCTACGTGCCACAACGACTGTTCTCAAGGAAGTTGAATAGAGCAAGTCGTCTACTTACAGCTTCAATTTCAACGGAGAGACCGGATTTGGAAGTGAAAGAGTGTGGAATACAACTAATATCCCAGCAAGGTGCTGCATTGTCTGTTCAAGATTTAACCCAGACAGCTGCTTATCAACTTCATCAGCTGGATCCAAATTTTGTGCTTCCTCCACATGTCGTAACAGTGGAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1126

Amino Acids

127.82

Weight (kDa)

6.52

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 4 - 176 1.2e-22 NB-ARC domain
NACHT PF05729 20 - 170 3.4e-07 NACHT domain
WHD_ROQ1 PF23282 247 - 314 2.5e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 531 - 616 4.4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 608 - 664 4e-06 Leucine-rich repeat region
LRR_14 PF23598 817 - 883 8.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 222, 410, 714, 1206
AccB1I GGYRCC 1 cut(s) 2168
AccB7I CCANNNNNTGG 3 cut(s) 1514, 1531, 2957
AccI GTMKAC 1 cut(s) 3196
AciI CCGC 3 cut(s) 1160, 1382, 2672
AcoI YGGCCR 1 cut(s) 2433
AcuI CTGAAG 4 cut(s) 502, 507, 2528, 3094
AcvI CACGTG 1 cut(s) 810
AfaI GTAC 8 cut(s) 930, 998, 1080, 1421, 1447, 2633, 2962, 2968
AfiI CCNNNNNNNGG 9 cut(s) 386, 833, 1129, 1514, 1531, 2020, 2957, 3125, 3126
AflII CTTAAG 2 cut(s) 1601, 1732
AflIII ACRYGT 5 cut(s) 744, 809, 1105, 2157, 3358
AhlI ACTAGT 1 cut(s) 2963
AjiI CACGTC 2 cut(s) 448, 2160
AjnI CCWGG 3 cut(s) 45, 391, 3093
AleI CACNNNNGTG 2 cut(s) 1734, 2545
AloI GAACNNNNNNTCC 2 cut(s) 2144, 2176
Alw21I GWGCWC 3 cut(s) 312, 453, 3141
Alw26I GTCTC 3 cut(s) 1526, 1602, 3217
Alw44I GTGCAC 1 cut(s) 449
AlwNI CAGNNNCTG 1 cut(s) 677
Ama87I CYCGRG 1 cut(s) 3119
AoxI GGCC 5 cut(s) 583, 1738, 2433, 2524, 3128
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 5 cut(s) 650, 1711, 1764, 3278, 3311
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
Asp700I GAANNNNTTC 2 cut(s) 515, 2242
AspA2I CCTAGG 1 cut(s) 1867
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 6 cut(s) 389, 1431, 1471, 2011, 2357, 3128
AsuC2I CCSGG 4 cut(s) 954, 1436, 3120, 3121
AsuHPI GGTGA 4 cut(s) 748, 1496, 2161, 2420
AsuII TTCGAA 1 cut(s) 2447
AvaI CYCGRG 1 cut(s) 3119
AvaII GGWCC 5 cut(s) 389, 1431, 1471, 2011, 2357
AvrII CCTAGG 1 cut(s) 1867
AxyI CCTNAGG 2 cut(s) 1187, 2164
BaeGI GKGCMC 1 cut(s) 453
BaeI ACNNNNGTAYC 2 cut(s) 3097, 3130
BalI TGGCCA 1 cut(s) 2435
BamHI GGATCC 2 cut(s) 1162, 3334
BanI GGYRCC 1 cut(s) 2168
BauI CACGAG 1 cut(s) 1071
BbrPI CACGTG 1 cut(s) 810
BbsI GAAGAC 1 cut(s) 1775
Bbv12I GWGCWC 3 cut(s) 312, 453, 3141
BbvI GCAGC 5 cut(s) 662, 1698, 1751, 3265, 3298
BccI CCATC 9 cut(s) 1306, 1592, 1685, 2170, 2194, 2425, 2880, 2951, 2968
BceAI ACGGC 1 cut(s) 1332
BciT130I CCWGG 3 cut(s) 47, 393, 3095
BclI TGATCA 1 cut(s) 1849
BcnI CCSGG 4 cut(s) 954, 1436, 3120, 3121
BcoDI GTCTC 3 cut(s) 1526, 1602, 3217
BcuI ACTAGT 1 cut(s) 2963
BfrI CTTAAG 2 cut(s) 1601, 1732
BglII AGATCT 2 cut(s) 801, 2322
BisI GCNGC 6 cut(s) 651, 1712, 1765, 2672, 3279, 3312
BlnI CCTAGG 1 cut(s) 1867
BlsI GCNGC 6 cut(s) 652, 1713, 1766, 2673, 3280, 3313
BmcAI AGTACT 1 cut(s) 1421
Bme1390I CCNGG 7 cut(s) 47, 393, 954, 1436, 3095, 3120, 3121
Bme18I GGWCC 5 cut(s) 389, 1431, 1471, 2011, 2357
BmeT110I CYCGRG 1 cut(s) 3119
BmgBI CACGTC 2 cut(s) 448, 2160
BmgT120I GGNCC 6 cut(s) 389, 1431, 1471, 2011, 2357, 3128
BmiI GGNNCC 6 cut(s) 1164, 1433, 2013, 2170, 2442, 3336
BmrFI CCNGG 7 cut(s) 47, 393, 954, 1436, 3095, 3120, 3121
BoxI GACNNNNGTC 1 cut(s) 1610
BpiI GAAGAC 1 cut(s) 1775
Bpu10I CCTNAGC 2 cut(s) 1124, 2007
Bpu14I TTCGAA 1 cut(s) 2447
BpuEI CTTGAG 6 cut(s) 1027, 1316, 1762, 2173, 2197, 3154
BpuMI CCSGG 4 cut(s) 954, 1436, 3120, 3121
BsaAI YACGTR 3 cut(s) 810, 2970, 3151
BsaI GGTCTC 2 cut(s) 1526, 3217
BsaWI WCCGGW 1 cut(s) 3226
Bsc4I CCNNNNNNNGG 9 cut(s) 386, 833, 1129, 1514, 1531, 2020, 2957, 3125, 3126
Bse1I ACTGG 4 cut(s) 579, 1417, 2239, 2903
Bse21I CCTNAGG 2 cut(s) 1187, 2164
Bse3DI GCAATG 1 cut(s) 756
BseBI CCWGG 3 cut(s) 47, 393, 3095
BseGI GGATG 7 cut(s) 55, 823, 897, 1072, 1628, 2233, 2979
BseLI CCNNNNNNNGG 9 cut(s) 386, 833, 1129, 1514, 1531, 2020, 2957, 3125, 3126
BseMI GCAATG 1 cut(s) 756
BseMII CTCAG 3 cut(s) 509, 2021, 2666
BseNI ACTGG 4 cut(s) 579, 1417, 2239, 2903
BseRI GAGGAG 2 cut(s) 789, 2768
BseSI GKGCMC 1 cut(s) 453
BseXI GCAGC 5 cut(s) 662, 1698, 1751, 3265, 3298
BseYI CCCAGC 1 cut(s) 3267
BshFI GGCC 5 cut(s) 585, 1740, 2435, 2526, 3130
BshNI GGYRCC 1 cut(s) 2168
BsiHKAI GWGCWC 3 cut(s) 312, 453, 3141
BsiHKCI CYCGRG 1 cut(s) 3119
BsiSI CCGG 4 cut(s) 953, 1436, 3120, 3227
BslFI GGGAC 3 cut(s) 1708, 2055, 2292
BslI CCNNNNNNNGG 9 cut(s) 386, 833, 1129, 1514, 1531, 2020, 2957, 3125, 3126
BsmAI GTCTC 3 cut(s) 1526, 1602, 3217
BsmFI GGGAC 3 cut(s) 1708, 2055, 2292
BsmI GAATGC 3 cut(s) 991, 1162, 1288
BsnI GGCC 5 cut(s) 585, 1740, 2435, 2526, 3130
Bso31I GGTCTC 2 cut(s) 1526, 3217
BsoBI CYCGRG 1 cut(s) 3119
Bsp119I TTCGAA 1 cut(s) 2447
Bsp1286I GDGCHC 3 cut(s) 312, 453, 3141
BspACI CCGC 3 cut(s) 1160, 1382, 2672
BspANI GGCC 5 cut(s) 585, 1740, 2435, 2526, 3130
BspCNI CTCAG 3 cut(s) 508, 2020, 2667
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 6 cut(s) 1164, 1433, 2013, 2170, 2442, 3336
BspT104I TTCGAA 1 cut(s) 2447
BspT107I GGYRCC 1 cut(s) 2168
BspTI CTTAAG 2 cut(s) 1601, 1732
BspTNI GGTCTC 2 cut(s) 1526, 3217
BsrDI GCAATG 1 cut(s) 756
BsrI ACTGG 4 cut(s) 579, 1417, 2239, 2903
BssSI CACGAG 1 cut(s) 1071
BssT1I CCWWGG 4 cut(s) 380, 1867, 2404, 2436
Bst2BI CACGAG 1 cut(s) 1071
Bst2UI CCWGG 3 cut(s) 47, 393, 3095
Bst4CI ACNGT 8 cut(s) 736, 888, 1032, 1450, 2060, 2069, 3165, 3370
Bst6I CTCTTC 1 cut(s) 1137
BstAFI CTTAAG 2 cut(s) 1601, 1732
BstAPI GCANNNNNTGC 1 cut(s) 1294
BstBAI YACGTR 3 cut(s) 810, 2970, 3151
BstBI TTCGAA 1 cut(s) 2447
BstC8I GCNNGC 1 cut(s) 2004
BstF5I GGATG 7 cut(s) 55, 823, 897, 1072, 1628, 2233, 2979
BstHHI GCGC 1 cut(s) 152
BstMAI GTCTC 3 cut(s) 1526, 1602, 3217
BstMWI GCNNNNNNNGC 5 cut(s) 158, 656, 1294, 2620, 3136
BstNI CCWGG 3 cut(s) 47, 393, 3095
BstNSI RCATGY 4 cut(s) 748, 765, 1109, 3362
BstPAI GACNNNNGTC 1 cut(s) 1610
BstSCI CCNGG 7 cut(s) 45, 391, 952, 1434, 3093, 3118, 3119
BstSLI GKGCMC 1 cut(s) 453
BstV1I GCAGC 5 cut(s) 662, 1698, 1751, 3265, 3298
BstV2I GAAGAC 1 cut(s) 1775
BstXI CCANNNNNNTGG 1 cut(s) 1591
Bsu36I CCTNAGG 2 cut(s) 1187, 2164
BsuRI GGCC 5 cut(s) 585, 1740, 2435, 2526, 3130
BtrI CACGTC 2 cut(s) 448, 2160
BtsCI GGATG 7 cut(s) 55, 823, 897, 1072, 1628, 2233, 2979
BtsIMutI CAGTG 3 cut(s) 586, 2552, 3375
Cac8I GCNNGC 1 cut(s) 2004
CaiI CAGNNNCTG 1 cut(s) 677
CciI TCATGA 1 cut(s) 331
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 6 cut(s) 389, 1431, 1471, 2011, 2357, 3128
Cfr9I CCCGGG 1 cut(s) 3119
CseI GACGC 1 cut(s) 2289
Csp6I GTAC 8 cut(s) 929, 997, 1079, 1420, 1446, 2632, 2961, 2967
CviQI GTAC 8 cut(s) 929, 997, 1079, 1420, 1446, 2632, 2961, 2967
DraI TTTAAA 2 cut(s) 2811, 3043
EaeI YGGCCR 1 cut(s) 2433
Eam1104I CTCTTC 1 cut(s) 1137
EarI CTCTTC 1 cut(s) 1137
Eco130I CCWWGG 4 cut(s) 380, 1867, 2404, 2436
Eco31I GGTCTC 2 cut(s) 1526, 3217
Eco32I GATATC 3 cut(s) 1818, 2509, 2797
Eco47I GGWCC 5 cut(s) 389, 1431, 1471, 2011, 2357
Eco57I CTGAAG 4 cut(s) 502, 507, 2528, 3094
Eco72I CACGTG 1 cut(s) 810
Eco81I CCTNAGG 2 cut(s) 1187, 2164
Eco88I CYCGRG 1 cut(s) 3119
EcoO109I RGGNCCY 2 cut(s) 1431, 2011
EcoRI GAATTC 2 cut(s) 4, 2889
EcoRII CCWGG 3 cut(s) 45, 391, 3093
EcoRV GATATC 3 cut(s) 1818, 2509, 2797
EcoT14I CCWWGG 4 cut(s) 380, 1867, 2404, 2436
EcoT22I ATGCAT 2 cut(s) 897, 1089
ErhI CCWWGG 4 cut(s) 380, 1867, 2404, 2436
FalI AAGNNNNNCTT 4 cut(s) 2397, 2429, 3066, 3098
FaqI GGGAC 3 cut(s) 1708, 2055, 2292
FbaI TGATCA 1 cut(s) 1849
FblI GTMKAC 1 cut(s) 3196
Fnu4HI GCNGC 6 cut(s) 651, 1712, 1765, 2672, 3279, 3312
FokI GGATG 7 cut(s) 62, 810, 904, 1079, 1635, 2240, 2986
Fsp4HI GCNGC 6 cut(s) 651, 1712, 1765, 2672, 3279, 3312
GlaI GCGC 1 cut(s) 151
GluI GCNGC 6 cut(s) 651, 1712, 1765, 2672, 3279, 3312
GsaI CCCAGC 1 cut(s) 3271
HaeIII GGCC 5 cut(s) 585, 1740, 2435, 2526, 3130
HapII CCGG 4 cut(s) 953, 1436, 3120, 3227
HgaI GACGC 1 cut(s) 2289
HhaI GCGC 1 cut(s) 152
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 3 cut(s) 202, 732, 844
HindII GTYRAC 3 cut(s) 202, 732, 844
HindIII AAGCTT 5 cut(s) 489, 1126, 1373, 1457, 1789
HpaII CCGG 4 cut(s) 953, 1436, 3120, 3227
HphI GGTGA 4 cut(s) 748, 1496, 2161, 2420
Hpy99I CGWCG 1 cut(s) 1983
HpyCH4III ACNGT 8 cut(s) 736, 888, 1032, 1450, 2060, 2069, 3165, 3370
HpyCH4IV ACGT 9 cut(s) 295, 447, 809, 931, 961, 2159, 2698, 2969, 3150
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 656, 1294, 2620, 3136
HpySE526I ACGT 9 cut(s) 295, 447, 809, 931, 961, 2159, 2698, 2969, 3150
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 1849
LmnI GCTCC 3 cut(s) 538, 1708, 1872
Lsp1109I GCAGC 5 cut(s) 662, 1698, 1751, 3265, 3298
MaeII ACGT 9 cut(s) 295, 447, 809, 931, 961, 2159, 2698, 2969, 3150
MaeIII GTNAC 9 cut(s) 815, 1146, 1985, 2230, 2327, 2879, 2929, 3113, 3364
MfeI CAATTG 2 cut(s) 63, 1512
MhlI GDGCHC 3 cut(s) 312, 453, 3141
MlsI TGGCCA 1 cut(s) 2435
MluNI TGGCCA 1 cut(s) 2435
MlyI GAGTC 4 cut(s) 35, 1574, 1604, 2686
MmeI TCCRAC 5 cut(s) 148, 560, 582, 1549, 1656
Mox20I TGGCCA 1 cut(s) 2435
Mph1103I ATGCAT 2 cut(s) 897, 1089
MroXI GAANNNNTTC 2 cut(s) 515, 2242
MscI TGGCCA 1 cut(s) 2435
MslI CAYNNNNRTG 5 cut(s) 240, 1360, 1734, 2047, 2545
Msp20I TGGCCA 1 cut(s) 2435
MspA1I CMGCKG 3 cut(s) 2674, 3311, 3331
MspCI CTTAAG 2 cut(s) 1601, 1732
MspI CCGG 4 cut(s) 953, 1436, 3120, 3227
MspR9I CCNGG 7 cut(s) 47, 393, 954, 1436, 3095, 3120, 3121
MunI CAATTG 2 cut(s) 63, 1512
Mva1269I GAATGC 3 cut(s) 991, 1162, 1288
MvaI CCWGG 3 cut(s) 47, 393, 3095
MwoI GCNNNNNNNGC 5 cut(s) 158, 656, 1294, 2620, 3136
NciI CCSGG 4 cut(s) 954, 1436, 3120, 3121
NlaIV GGNNCC 6 cut(s) 1164, 1433, 2013, 2170, 2442, 3336
NmuCI GTSAC 3 cut(s) 815, 2327, 2879
NsiI ATGCAT 2 cut(s) 897, 1089
NspI RCATGY 4 cut(s) 748, 765, 1109, 3362
NspV TTCGAA 1 cut(s) 2447
OliI CACNNNNGTG 2 cut(s) 1734, 2545
PagI TCATGA 1 cut(s) 331
PciI ACATGT 3 cut(s) 744, 1105, 3358
PctI GAATGC 3 cut(s) 991, 1162, 1288
PdmI GAANNNNTTC 2 cut(s) 515, 2242
PfeI GAWTC 7 cut(s) 376, 938, 2204, 2243, 2372, 2400, 2844
PflMI CCANNNNNTGG 3 cut(s) 1514, 1531, 2957
PkrI GCNGC 6 cut(s) 652, 1713, 1766, 2673, 3280, 3313
PleI GAGTC 4 cut(s) 34, 1574, 1603, 2685
PmaCI CACGTG 1 cut(s) 810
PmlI CACGTG 1 cut(s) 810
PpsI GAGTC 4 cut(s) 34, 1574, 1603, 2685
Ppu21I YACGTR 3 cut(s) 810, 2970, 3151
PpuMI RGGWCCY 2 cut(s) 1431, 2011
PscI ACATGT 3 cut(s) 744, 1105, 3358
PshAI GACNNNNGTC 1 cut(s) 1610
PsiI TTATAA 4 cut(s) 222, 410, 714, 1206
Psp5II RGGWCCY 2 cut(s) 1431, 2011
Psp6I CCWGG 3 cut(s) 45, 391, 3093
PspCI CACGTG 1 cut(s) 810
PspFI CCCAGC 1 cut(s) 3267
PspGI CCWGG 3 cut(s) 45, 391, 3093
PspN4I GGNNCC 6 cut(s) 1164, 1433, 2013, 2170, 2442, 3336
PspPI GGNCC 6 cut(s) 389, 1431, 1471, 2011, 2357, 3128
PspPPI RGGWCCY 2 cut(s) 1431, 2011
PstNI CAGNNNCTG 1 cut(s) 677
PvuII CAGCTG 2 cut(s) 3311, 3331
RsaI GTAC 8 cut(s) 930, 998, 1080, 1421, 1447, 2633, 2962, 2968
RsaNI GTAC 8 cut(s) 929, 997, 1079, 1420, 1446, 2632, 2961, 2967
RseI CAYNNNNRTG 5 cut(s) 240, 1360, 1734, 2047, 2545
SatI GCNGC 6 cut(s) 651, 1712, 1765, 2672, 3279, 3312
Sau96I GGNCC 6 cut(s) 389, 1431, 1471, 2011, 2357, 3128
ScaI AGTACT 1 cut(s) 1421
SchI GAGTC 4 cut(s) 35, 1574, 1604, 2686
ScrFI CCNGG 7 cut(s) 47, 393, 954, 1436, 3095, 3120, 3121
SduI GDGCHC 3 cut(s) 312, 453, 3141
SfuI TTCGAA 1 cut(s) 2447
SinI GGWCC 5 cut(s) 389, 1431, 1471, 2011, 2357
SmaI CCCGGG 1 cut(s) 3121
SmiI ATTTAAAT 1 cut(s) 2811
SmiMI CAYNNNNRTG 5 cut(s) 240, 1360, 1734, 2047, 2545
SmlI CTYRAG 8 cut(s) 1006, 1331, 1601, 1732, 1777, 2188, 2212, 3169
SmoI CTYRAG 8 cut(s) 1006, 1331, 1601, 1732, 1777, 2188, 2212, 3169
SpeI ACTAGT 1 cut(s) 2963
SsiI CCGC 3 cut(s) 1160, 1382, 2672
SspI AATATT 1 cut(s) 275
StyD4I CCNGG 7 cut(s) 45, 391, 952, 1434, 3093, 3118, 3119
StyI CCWWGG 4 cut(s) 380, 1867, 2404, 2436
SwaI ATTTAAAT 1 cut(s) 2811
TaaI ACNGT 8 cut(s) 736, 888, 1032, 1450, 2060, 2069, 3165, 3370
TaiI ACGT 9 cut(s) 298, 450, 812, 934, 964, 2162, 2701, 2972, 3153
TaqI TCGA 8 cut(s) 1034, 1476, 1981, 2447, 2579, 2799, 2842, 3103
TaqII GACCGA 1 cut(s) 2640
TatI WGTACW 3 cut(s) 1078, 1419, 1445
TauI GCSGC 1 cut(s) 2674
TfiI GAWTC 7 cut(s) 376, 938, 2204, 2243, 2372, 2400, 2844
TscAI CASTG 3 cut(s) 586, 2552, 3375
TseFI GTSAC 3 cut(s) 815, 2327, 2879
TseI GCWGC 5 cut(s) 650, 1711, 1764, 3278, 3311
Tsp45I GTSAC 3 cut(s) 815, 2327, 2879
TspGWI ACGGA 3 cut(s) 1155, 1967, 3233
TspMI CCCGGG 1 cut(s) 3119
TspRI CASTG 3 cut(s) 586, 2552, 3375
Van91I CCANNNNNTGG 3 cut(s) 1514, 1531, 2957
Vha464I CTTAAG 2 cut(s) 1601, 1732
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 5 cut(s) 389, 1431, 1471, 2011, 2357
XbaI TCTAGA 2 cut(s) 319, 1687
XceI RCATGY 4 cut(s) 748, 765, 1109, 3362
XmaI CCCGGG 1 cut(s) 3119
XmaJI CCTAGG 1 cut(s) 1867
XmiI GTMKAC 1 cut(s) 3196
XmnI GAANNNNTTC 2 cut(s) 515, 2242
ZrmI AGTACT 1 cut(s) 1421
Zsp2I ATGCAT 2 cut(s) 897, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.