RchiOBHm_Chr6g0251631

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000307
Physical Location & Seq
Reverse (-)
6895151 .. 6898477
3327 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22558

Sequence Viewer

Length: 2334 bp
ATGAACTCGTTGCTGGACAAAAGATCAGGAAAGTTGGAAGAACAAGAGGCTCCCAAGAAGGGTAAACAAGTTCTAGAATCTGACTTCAACATGCACCCGAGAGAAGTTCTACAGTCGTTGCTTTCAGTACTCTATGAGGGACCATATGGACGTGAAAAGGACTTTGGCCTTTTTTTTCCTCATAAGGTAGTTCCTTTCTGGTTTTTCCATCACTATGCAGGGGACGTGGCACTGTGCGACTTTCCTCTGGACTTATCTGTTGATCAGAGATGGGTCGGTTTAGAACTATTTGTTATGGTTTCGAGTGGTTACACTATCAATGTTGAATCCTTTCTTGATGTTGATTTGTGTTCCAATGAAATTAGCACAGTAAGCACATCCGTAATGATAAACATTTGTCTGGGGTCAGATCAACTTGTTGTAATCCATATACCACGAGTGCGTTTTCCGGAGCAGTTGAATCAATGTCACGGTATCAGCGCATTGTTTAGAACCACCACTCCAGATATGAGGGTTCGAATGTGTGGAAGCCGTTTACTATACGAGCAAGATTTGGAAGACTTGATCCGTGCAATAACTGTGTGCACATTAGACAGTCAAGATGTCATTGATTCACTTTGTCAGCACATCCAACAATTACTTGGAAGTAACTTGCTGGAGGAACCTTCTGATCATGTAGTAGCAGTTGAAACCGTTGAGCAGAATTCAGTGGAAGGAGAAACTGAGGTTGGTCGCAGAAATGATCATTCCTCCACTGAAAGGGAGGACTTGATTCAGGAACGGAGAGAGAAAGTCTTCACTTCAATATTGCACAACTACAGATCTTATCTCCAACAAAAGCATGGTTGTATAGAGTCGGAAAGAATTATATCCCATTCTGAAGGCTATCTTTTGGACGATGGAGATCCAAAGCCGGTGTTTGCCACGCATCATTTACATGTAATGGTTGAGACTCGACTCTATGGGAGCCTTAGCTCAGAAGATTGGAAACAATCTCTCGAGTCATTGCTTCAGTATTTCAAGGGGGCCACTCTCAGTGTCAAAGGACAAATGATTGCTGTTTTAAATCCGTTCACTCCTTCCTCAGTAAACAACTTTTGTTTCCCTCGAAAGGAACTTCCAGGATGGTTCACGCAACAGACGAGAATGCCCAGGATTGGATTGATCATAGACCCACTTGTATGTGATAATGAGAATTGGATGGGATTTGCTGTGTGCGCTACTTTTTCAGTCCACGAGCATCCAAGTGCTGTCCTTGACAACCTGGCCACAAGGACTTTCAACGTTATGTGTCATTTGGCAGCATCGGATGGACGATGTTTGAGTCCTGCCCCGGTGTTTACCATAACAAAAGAGAAATTCAAGTGGCTACATCTTCGTGGATTTATGTGGCTAACCTACATACCTCGTACTTATCTTGCGGAGTTCAGAGAAGTACGTGATGCAGAAGCTAGAATTTATAATGATTGCCCAGGATTGATGATGACAAGGTGGAGTATCCGTTCCTTATTCAAGCAAGATGTGAAGGAATTTAATAAAGCAATAGTCCAGTGCTGGACTTCGTTCTTCGACAATTTGGATCTCATCCGTCAGTTTGTCGAGGCAGATGAAAATGAACAGTCACATTATTCTGATAGAGGGGAAACCAGTACCAGTAAAATCTTGACGCCCAAAAGACATGTGAAGGATTTCAATCGGGACTTCAAATATAATGCAACTTACCCTCATTGCGCTAAACTGTTGGAGTGGTTCGGTGATCACACAAGGGGCCCTTCAGTGAAAATCCCGTTACCACCATCTCTATCTAGTGATGGGAATTGGATAGGATTAGCTCTATATGCACGTTTTTCAGACCTCGAGAATCAAACTACCTCCAAAGACAAAGTGAATCCAGAAATTGTTGAGCTTGTTTGTCATTTGGAAACTGAGAACGGTAGTTTTAAACATCTTCACCACTATCAACCAACCTATGAAGAATTCAAGCGGTTATGTCCTACAGAATTCATTTGGCTATCTTACATACCACGTCAGGGGTATCTAGATCAGTTAAATGAGTGTGACCTCTTGGAGGCTTCATTTGCTAGCGAAAGCCGAGGGTTGTTTGCTGAAGTGTGTGTTCTCCGTCTTGTCTACCAGCATAATGAGCAAGAGTTTAAGGATCTGTGTATCTCCATGAACTCGTTGCTGGACAAAAGATCAGGAAAGTTGGAAGAACAAGAGGCTCCCAAGAAGGGTAAACAAGTTCTGGAACACCCTGAACCTGAGATTGTATGGGGATCTAATGCAGAGTGGAATCTGGAGATCTGCGACGACCCAAAAACGAGACGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

777

Amino Acids

89.6

Weight (kDa)

5.49

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 368 - 508 9e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1461
AatII GACGTC 1 cut(s) 2329
AccI GTMKAC 1 cut(s) 2130
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 2 cut(s) 1421, 1984
AclI AACGTT 1 cut(s) 1284
AclWI GGATC 5 cut(s) 559, 899, 1587, 2166, 2284
AcoI YGGCCR 1 cut(s) 1266
AcsI RAATTY 6 cut(s) 703, 1358, 1455, 1529, 1976, 2000
AcuI CTGAAG 4 cut(s) 900, 995, 1758, 2127
AcyI GRCGYC 2 cut(s) 1667, 2326
AfaI GTAC 4 cut(s) 129, 1411, 1437, 1651
AfiI CCNNNNNNNGG 7 cut(s) 59, 759, 1111, 1157, 2030, 2068, 2231
AflIII ACRYGT 2 cut(s) 937, 1678
AjiI CACGTC 3 cut(s) 152, 226, 2027
AjnI CCWGG 4 cut(s) 1120, 1151, 1263, 1471
AjuI GAANNNNNNNTTGG 4 cut(s) 147, 179, 711, 743
AloI GAACNNNNNNTCC 4 cut(s) 484, 516, 1486, 1518
AluBI AGCT 4 cut(s) 975, 1451, 1832, 1906
AluI AGCT 4 cut(s) 975, 1451, 1832, 1906
Alw21I GWGCWC 1 cut(s) 587
Alw26I GTCTC 2 cut(s) 944, 2317
Alw44I GTGCAC 1 cut(s) 583
AlwI GGATC 5 cut(s) 559, 899, 1587, 2166, 2284
Ama87I CYCGRG 3 cut(s) 97, 998, 1856
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 4 cut(s) 166, 1026, 1266, 1768
ApaI GGGCCC 1 cut(s) 1772
ApaLI GTGCAC 1 cut(s) 583
ApeKI GCWGC 1 cut(s) 1301
ApoI RAATTY 6 cut(s) 703, 1358, 1455, 1529, 1976, 2000
Asp700I GAANNNNTTC 3 cut(s) 330, 794, 1688
AspLEI GCGC 3 cut(s) 482, 1220, 1733
AspS9I GGNCC 4 cut(s) 140, 1026, 1768, 1769
AsuC2I CCSGG 1 cut(s) 1334
AsuHPI GGTGA 2 cut(s) 1766, 1943
AsuII TTCGAA 1 cut(s) 517
AsuNHI GCTAGC 1 cut(s) 2081
AvaI CYCGRG 3 cut(s) 97, 998, 1856
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 2 cut(s) 587, 1772
BalI TGGCCA 1 cut(s) 1268
BanII GRGCYC 1 cut(s) 1772
BauI CACGAG 2 cut(s) 435, 1235
BbsI GAAGAC 2 cut(s) 564, 787
Bbv12I GWGCWC 1 cut(s) 587
BbvI GCAGC 1 cut(s) 1313
BccI CCATC 8 cut(s) 216, 264, 893, 1119, 1195, 1304, 1804, 1805
BceAI ACGGC 1 cut(s) 516
BciT130I CCWGG 4 cut(s) 1122, 1153, 1265, 1473
BciVI GTATCC 1 cut(s) 1508
BclI TGATCA 5 cut(s) 262, 670, 742, 1164, 1756
BcnI CCSGG 1 cut(s) 1334
BcoDI GTCTC 2 cut(s) 944, 2317
BfaI CTAG 5 cut(s) 74, 1452, 1806, 2039, 2082
BfmI CTRYAG 3 cut(s) 110, 817, 1995
BfuI GTATCC 1 cut(s) 1508
BglII AGATCT 2 cut(s) 821, 2301
BisI GCNGC 1 cut(s) 1302
BlsI GCNGC 1 cut(s) 1303
BmcAI AGTACT 1 cut(s) 129
Bme1390I CCNGG 5 cut(s) 1122, 1153, 1265, 1334, 1473
Bme18I GGWCC 1 cut(s) 140
BmeT110I CYCGRG 3 cut(s) 97, 998, 1856
BmgBI CACGTC 3 cut(s) 152, 226, 2027
BmgT120I GGNCC 4 cut(s) 140, 1026, 1768, 1769
BmiI GGNNCC 8 cut(s) 51, 141, 663, 968, 1027, 1769, 1770, 2223
BmrFI CCNGG 5 cut(s) 1122, 1153, 1265, 1334, 1473
BmsI GCATC 4 cut(s) 937, 1249, 1313, 1432
BmtI GCTAGC 1 cut(s) 2085
BpiI GAAGAC 2 cut(s) 564, 787
BpmI CTGGAG 3 cut(s) 486, 677, 2318
Bpu10I CCTNAGC 1 cut(s) 971
Bpu14I TTCGAA 1 cut(s) 517
BpuMI CCSGG 1 cut(s) 1334
BsaAI YACGTR 1 cut(s) 1439
BsaBI GATNNNNATC 2 cut(s) 903, 1692
BsaHI GRCGYC 2 cut(s) 1667, 2326
BsaJI CCNNGG 4 cut(s) 1151, 1332, 1471, 2092
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 4 cut(s) 484, 514, 1486, 1516
Bsc4I CCNNNNNNNGG 7 cut(s) 59, 759, 1111, 1157, 2030, 2068, 2231
Bse118I RCCGGY 1 cut(s) 913
Bse1I ACTGG 3 cut(s) 1549, 1647, 1653
Bse3DI GCAATG 2 cut(s) 1004, 1726
Bse8I GATNNNNATC 2 cut(s) 903, 1692
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 4 cut(s) 1122, 1153, 1265, 1473
BseDI CCNNGG 4 cut(s) 1151, 1332, 1471, 2092
BseGI GGATG 7 cut(s) 377, 627, 1130, 1206, 1240, 1315, 1584
BseJI GATNNNNATC 2 cut(s) 903, 1692
BseLI CCNNNNNNNGG 7 cut(s) 59, 759, 1111, 1157, 2030, 2068, 2231
BseMI GCAATG 2 cut(s) 1004, 1726
BseMII CTCAG 6 cut(s) 714, 990, 1048, 1098, 1917, 2253
BseNI ACTGG 3 cut(s) 1549, 1647, 1653
BseSI GKGCMC 2 cut(s) 587, 1772
BseXI GCAGC 1 cut(s) 1313
BshFI GGCC 4 cut(s) 168, 1028, 1268, 1770
BsiHKAI GWGCWC 1 cut(s) 587
BsiHKCI CYCGRG 3 cut(s) 97, 998, 1856
BsiSI CCGG 3 cut(s) 449, 914, 1334
BslFI GGGAC 3 cut(s) 153, 236, 1712
BslI CCNNNNNNNGG 7 cut(s) 59, 759, 1111, 1157, 2030, 2068, 2231
BsmAI GTCTC 2 cut(s) 944, 2317
BsmBI CGTCTC 1 cut(s) 2317
BsmFI GGGAC 3 cut(s) 153, 236, 1712
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 4 cut(s) 168, 1028, 1268, 1770
BsoBI CYCGRG 3 cut(s) 97, 998, 1856
Bsp119I TTCGAA 1 cut(s) 517
Bsp120I GGGCCC 1 cut(s) 1768
Bsp1286I GDGCHC 2 cut(s) 587, 1772
Bsp13I TCCGGA 1 cut(s) 448
BspACI CCGC 2 cut(s) 1421, 1984
BspANI GGCC 4 cut(s) 168, 1028, 1268, 1770
BspCNI CTCAG 6 cut(s) 715, 989, 1047, 1097, 1918, 2254
BspEI TCCGGA 1 cut(s) 448
BspLI GGNNCC 8 cut(s) 51, 141, 663, 968, 1027, 1769, 1770, 2223
BspOI GCTAGC 1 cut(s) 2085
BspPI GGATC 5 cut(s) 559, 899, 1587, 2166, 2284
BspT104I TTCGAA 1 cut(s) 517
BsrDI GCAATG 2 cut(s) 1004, 1726
BsrFI RCCGGY 1 cut(s) 913
BsrI ACTGG 3 cut(s) 1549, 1647, 1653
BssAI RCCGGY 1 cut(s) 913
BssECI CCNNGG 4 cut(s) 1151, 1332, 1471, 2092
BssNI GRCGYC 2 cut(s) 1667, 2326
BssSI CACGAG 2 cut(s) 435, 1235
Bst2BI CACGAG 2 cut(s) 435, 1235
Bst2UI CCWGG 4 cut(s) 1122, 1153, 1265, 1473
BstACI GRCGYC 2 cut(s) 1667, 2326
BstBAI YACGTR 1 cut(s) 1439
BstBI TTCGAA 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 2083
BstDEI CTNAG 7 cut(s) 723, 971, 976, 1034, 1084, 1926, 2262
BstENI CCTNNNNNAGG 1 cut(s) 2066
BstF5I GGATG 7 cut(s) 377, 627, 1130, 1206, 1240, 1315, 1584
BstHHI GCGC 3 cut(s) 482, 1220, 1733
BstMAI GTCTC 2 cut(s) 944, 2317
BstMWI GCNNNNNNNGC 5 cut(s) 372, 1217, 1838, 2078, 2143
BstNI CCWGG 4 cut(s) 1122, 1153, 1265, 1473
BstNSI RCATGY 3 cut(s) 94, 941, 1682
BstSCI CCNGG 5 cut(s) 1120, 1151, 1263, 1332, 1471
BstSFI CTRYAG 3 cut(s) 110, 817, 1995
BstSLI GKGCMC 2 cut(s) 587, 1772
BstV1I GCAGC 1 cut(s) 1313
BstV2I GAAGAC 2 cut(s) 564, 787
BstX2I RGATCY 6 cut(s) 821, 904, 1579, 2158, 2276, 2301
BstYI RGATCY 6 cut(s) 821, 904, 1579, 2158, 2276, 2301
BsuI GTATCC 1 cut(s) 1508
BsuRI GGCC 4 cut(s) 168, 1028, 1268, 1770
BtrI CACGTC 3 cut(s) 152, 226, 2027
BtsCI GGATG 7 cut(s) 377, 627, 1130, 1206, 1240, 1315, 1584
BtsIMutI CAGTG 6 cut(s) 230, 714, 753, 1042, 1556, 1782
Cac8I GCNNGC 1 cut(s) 2083
CfoI GCGC 3 cut(s) 482, 1220, 1733
Cfr10I RCCGGY 1 cut(s) 913
Cfr13I GGNCC 4 cut(s) 140, 1026, 1768, 1769
CseI GACGC 1 cut(s) 1675
Csp6I GTAC 4 cut(s) 128, 1410, 1436, 1650
CviAII CATG 6 cut(s) 91, 674, 842, 938, 1679, 2173
CviQI GTAC 4 cut(s) 128, 1410, 1436, 1650
DdeI CTNAG 7 cut(s) 723, 971, 976, 1034, 1084, 1926, 2262
DraI TTTAAA 2 cut(s) 1065, 1942
EaeI YGGCCR 1 cut(s) 1266
Eco24I GRGCYC 1 cut(s) 1772
Eco47I GGWCC 1 cut(s) 140
Eco57I CTGAAG 4 cut(s) 900, 995, 1758, 2127
Eco88I CYCGRG 3 cut(s) 97, 998, 1856
EcoNI CCTNNNNNAGG 1 cut(s) 2066
EcoO109I RGGNCCY 2 cut(s) 1768, 1769
EcoRI GAATTC 3 cut(s) 703, 1976, 2000
EcoRII CCWGG 4 cut(s) 1120, 1151, 1263, 1471
EcoT38I GRGCYC 1 cut(s) 1772
Esp3I CGTCTC 1 cut(s) 2317
FaeI CATG 6 cut(s) 94, 677, 845, 941, 1682, 2176
FalI AAGNNNNNCTT 4 cut(s) 873, 905, 1756, 1788
FaqI GGGAC 3 cut(s) 153, 236, 1712
FatI CATG 6 cut(s) 90, 673, 841, 937, 1678, 2172
FauNDI CATATG 1 cut(s) 145
FbaI TGATCA 5 cut(s) 262, 670, 742, 1164, 1756
FblI GTMKAC 1 cut(s) 2130
Fnu4HI GCNGC 1 cut(s) 1302
FokI GGATG 7 cut(s) 364, 614, 1137, 1213, 1227, 1322, 1571
FriOI GRGCYC 1 cut(s) 1772
Fsp4HI GCNGC 1 cut(s) 1302
FspBI CTAG 5 cut(s) 74, 1452, 1806, 2039, 2082
GlaI GCGC 3 cut(s) 481, 1219, 1732
GluI GCNGC 1 cut(s) 1302
GsuI CTGGAG 3 cut(s) 486, 677, 2318
HaeIII GGCC 4 cut(s) 168, 1028, 1268, 1770
HapII CCGG 3 cut(s) 449, 914, 1334
HgaI GACGC 1 cut(s) 1675
HhaI GCGC 3 cut(s) 482, 1220, 1733
Hin1I GRCGYC 2 cut(s) 1667, 2326
Hin1II CATG 6 cut(s) 94, 677, 845, 941, 1682, 2176
Hin6I GCGC 3 cut(s) 480, 1218, 1731
HinP1I GCGC 3 cut(s) 480, 1218, 1731
HpaII CCGG 3 cut(s) 449, 914, 1334
HphI GGTGA 2 cut(s) 1766, 1943
Hpy99I CGWCG 2 cut(s) 2312, 2331
HpyAV CCTTC 9 cut(s) 52, 675, 707, 875, 1089, 1519, 1678, 1782, 2224
HpyCH4IV ACGT 7 cut(s) 151, 225, 1284, 1438, 1843, 2026, 2326
HpyCH4V TGCA 9 cut(s) 94, 218, 572, 585, 811, 1445, 1715, 1841, 2285
HpyF10VI GCNNNNNNNGC 5 cut(s) 372, 1217, 1838, 2078, 2143
HpyF3I CTNAG 7 cut(s) 723, 971, 976, 1034, 1084, 1926, 2262
HpySE526I ACGT 7 cut(s) 151, 225, 1284, 1438, 1843, 2026, 2326
Hsp92I GRCGYC 2 cut(s) 1667, 2326
Hsp92II CATG 6 cut(s) 94, 677, 845, 941, 1682, 2176
HspAI GCGC 3 cut(s) 480, 1218, 1731
Kpn2I TCCGGA 1 cut(s) 448
Ksp22I TGATCA 5 cut(s) 262, 670, 742, 1164, 1756
LmnI GCTCC 4 cut(s) 55, 451, 966, 2227
Lsp1109I GCAGC 1 cut(s) 1313
LweI GCATC 4 cut(s) 937, 1249, 1313, 1432
MaeI CTAG 5 cut(s) 74, 1452, 1806, 2039, 2082
MaeII ACGT 7 cut(s) 151, 225, 1284, 1438, 1843, 2026, 2326
MaeIII GTNAC 6 cut(s) 308, 467, 647, 1620, 1788, 2057
MboII GAAGA 9 cut(s) 50, 569, 787, 992, 1367, 1558, 1940, 1985, 2222
MflI RGATCY 6 cut(s) 821, 904, 1579, 2158, 2276, 2301
MhlI GDGCHC 2 cut(s) 587, 1772
MlsI TGGCCA 1 cut(s) 1268
MluNI TGGCCA 1 cut(s) 1268
MlyI GAGTC 5 cut(s) 863, 946, 951, 1010, 1333
MmeI TCCRAC 6 cut(s) 15, 655, 837, 856, 1722, 2187
Mox20I TGGCCA 1 cut(s) 1268
MroI TCCGGA 1 cut(s) 448
MroXI GAANNNNTTC 3 cut(s) 330, 794, 1688
MscI TGGCCA 1 cut(s) 1268
MseI TTAA 5 cut(s) 1064, 1533, 1941, 2048, 2154
MslI CAYNNNNRTG 5 cut(s) 213, 936, 1180, 1245, 1377
Msp20I TGGCCA 1 cut(s) 1268
MspI CCGG 3 cut(s) 449, 914, 1334
MspR9I CCNGG 5 cut(s) 1122, 1153, 1265, 1334, 1473
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 4 cut(s) 1122, 1153, 1265, 1473
MwoI GCNNNNNNNGC 5 cut(s) 372, 1217, 1838, 2078, 2143
NciI CCSGG 1 cut(s) 1334
NdeI CATATG 1 cut(s) 145
NheI GCTAGC 1 cut(s) 2081
NlaIII CATG 6 cut(s) 94, 677, 845, 941, 1682, 2176
NlaIV GGNNCC 8 cut(s) 51, 141, 663, 968, 1027, 1769, 1770, 2223
NmeAIII GCCGAG 1 cut(s) 2117
NmuCI GTSAC 3 cut(s) 467, 1620, 2057
NspI RCATGY 3 cut(s) 94, 941, 1682
NspV TTCGAA 1 cut(s) 517
PaeR7I CTCGAG 2 cut(s) 998, 1856
PciI ACATGT 2 cut(s) 937, 1678
PcsI WCGNNNNNNNCGW 1 cut(s) 1139
PctI GAATGC 1 cut(s) 1152
PdmI GAANNNNTTC 3 cut(s) 330, 794, 1688
PfeI GAWTC 8 cut(s) 77, 326, 460, 611, 772, 1861, 1888, 2293
PfoI TCCNGGA 1 cut(s) 1120
PkrI GCNGC 1 cut(s) 1303
PleI GAGTC 5 cut(s) 862, 946, 951, 1009, 1332
PpsI GAGTC 5 cut(s) 862, 946, 951, 1009, 1332
Ppu21I YACGTR 1 cut(s) 1439
PscI ACATGT 2 cut(s) 937, 1678
PsiI TTATAA 1 cut(s) 1461
Psp1406I AACGTT 1 cut(s) 1284
Psp6I CCWGG 4 cut(s) 1120, 1151, 1263, 1471
PspGI CCWGG 4 cut(s) 1120, 1151, 1263, 1471
PspN4I GGNNCC 8 cut(s) 51, 141, 663, 968, 1027, 1769, 1770, 2223
PspOMI GGGCCC 1 cut(s) 1768
PspPI GGNCC 4 cut(s) 140, 1026, 1768, 1769
PsuI RGATCY 6 cut(s) 821, 904, 1579, 2158, 2276, 2301
RsaI GTAC 4 cut(s) 129, 1411, 1437, 1651
RsaNI GTAC 4 cut(s) 128, 1410, 1436, 1650
RseI CAYNNNNRTG 5 cut(s) 213, 936, 1180, 1245, 1377
SaqAI TTAA 5 cut(s) 1064, 1533, 1941, 2048, 2154
SatI GCNGC 1 cut(s) 1302
Sau96I GGNCC 4 cut(s) 140, 1026, 1768, 1769
ScaI AGTACT 1 cut(s) 129
SchI GAGTC 5 cut(s) 863, 946, 951, 1010, 1333
ScrFI CCNGG 5 cut(s) 1122, 1153, 1265, 1334, 1473
SduI GDGCHC 2 cut(s) 587, 1772
SfaNI GCATC 4 cut(s) 937, 1249, 1313, 1432
SfcI CTRYAG 3 cut(s) 110, 817, 1995
Sfr274I CTCGAG 2 cut(s) 998, 1856
SfuI TTCGAA 1 cut(s) 517
SinI GGWCC 1 cut(s) 140
SlaI CTCGAG 2 cut(s) 998, 1856
SmiMI CAYNNNNRTG 5 cut(s) 213, 936, 1180, 1245, 1377
SmlI CTYRAG 2 cut(s) 998, 1856
SmoI CTYRAG 2 cut(s) 998, 1856
SsiI CCGC 2 cut(s) 1421, 1984
SspI AATATT 1 cut(s) 807
SspMI CTAG 5 cut(s) 74, 1452, 1806, 2039, 2082
StyD4I CCNGG 5 cut(s) 1120, 1151, 1263, 1332, 1471
TaiI ACGT 7 cut(s) 154, 228, 1287, 1441, 1846, 2029, 2329
TaqI TCGA 8 cut(s) 302, 517, 955, 999, 1108, 1569, 1599, 1857
TatI WGTACW 1 cut(s) 127
TfiI GAWTC 8 cut(s) 77, 326, 460, 611, 772, 1861, 1888, 2293
Tru1I TTAA 5 cut(s) 1064, 1533, 1941, 2048, 2154
Tru9I TTAA 5 cut(s) 1064, 1533, 1941, 2048, 2154
TscAI CASTG 6 cut(s) 237, 714, 760, 1042, 1556, 1782
TseFI GTSAC 3 cut(s) 467, 1620, 2057
TseI GCWGC 1 cut(s) 1301
Tsp45I GTSAC 3 cut(s) 467, 1620, 2057
TspDTI ATGAA 8 cut(s) 17, 372, 1623, 1629, 1986, 1993, 2064, 2189
TspGWI ACGGA 7 cut(s) 370, 557, 796, 1059, 1490, 1577, 2111
TspRI CASTG 6 cut(s) 237, 714, 760, 1042, 1556, 1782
VneI GTGCAC 1 cut(s) 583
VpaK11BI GGWCC 1 cut(s) 140
XagI CCTNNNNNAGG 1 cut(s) 2066
XapI RAATTY 6 cut(s) 703, 1358, 1455, 1529, 1976, 2000
XbaI TCTAGA 2 cut(s) 73, 2038
XceI RCATGY 3 cut(s) 94, 941, 1682
XcmI CCANNNNNNNNNTGG 2 cut(s) 638, 839
XhoI CTCGAG 2 cut(s) 998, 1856
XmiI GTMKAC 1 cut(s) 2130
XmnI GAANNNNTTC 3 cut(s) 330, 794, 1688
XspI CTAG 5 cut(s) 74, 1452, 1806, 2039, 2082
ZraI GACGTC 1 cut(s) 2327
ZrmI AGTACT 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.