RchiOBHm_Chr2g0106541

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000307
Physical Location & Seq
Reverse (-)
17914161 .. 17917156
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48062

Sequence Viewer

Length: 2571 bp
ATGGGACTTGCTCTGTTTGTTGTTTTTGAAATCCTTGAAGGCAATTTCAATAAGATATGGGAATTGGAAGAGACTATCTGTGAATTTCACACCGATGTTGGTCCTGAAAATTCCCTTGTTTTTCAAAACTTCATAGACTTCAAGGCTGGGTCATATGCACTTTGTTGCTATGAACCGCGAGGGGGGCAATTTAGTGGGATGTTCGATAAACCAAGCTCACAACTTCGAGCTTCAGTTTCAATGAAGAGACCAAATTTAAAAGTGAGAGGTTGTGGGATACATCTAATGTCAGAGGAAGACACTGCAGAGTTTGTAAAAAGTTTAGCAAATCAGACTGCCACTCAACACCTCGATTCCAATTTCAATCAACATTGCGAGTACCTATTAAATGAGGAGACAACAACTGGTGACCTGATGGATGTGGGATCTACTTCAACTTTCAATGAAGATAGCTGCAGCAAAAGTTTCTCTAAATTCAAATTGAGAGGAGAGCTGTCGATACTCTATGAGGGATGCAATGGACGTGAGAATAGTTTCCATTTTTGCTTTCCTGCTCCCGTAATTTCAGCCCCACCCTGGTTCTTCCATCACCATGCAGGGGATGTAACTTTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCGGTGTACATCTACTTCTACATCTGGCAATAGTAGTTTTTTCTTTTATGTTGATATTTGCTCTCATGATCATGAAAGTATAGTAAGGCACGGATCCCTCAAGATAAACAGTTGTGTTGGGACCTCAGATCAACTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAGCAGTTGAATCAATGTCAGGGTATTAGCGCATTGTTCAGAACCATAGATGCAGAGATGGATGTCCAAGTGTGTGGAAGCCGTTTAGCTTTCGAGCATGATTTGGAAGACTTGATCCATTCATTAACTGCTGCTGTCGGCACGTTGGGGCAACATGTCCTTACTCAACCACGCTCCCCGGCCCAACCTGTTGATCGGCGCAATGCAGAGGAAGCAGAAATGCCCATCAATTGCTGTTCCTGTTTTCAGAGATGCAGAAGTACTGCACTAGTACTACCAGAACAAGCTCCTTCTTCTTCAATTGTGCAGCTGCGAAATTATAGATGCTATCTCCAGCAACAGCAAGGTTTTGGGGAGTCTGACGTTAACCAGTTGGTCCACTTCAGATCAGTAATGTTACTCCACTCCAGAAGCCTTTTGGAAAACAGGGACCGAAACCAAGAAAACATTGCCGAAAAGGATAAATCGTTGGTGTTGGCTCATCATCATGTACATATAATGGCTGAGACTCAACTTCAGGGAAGCTATAGTTCACCACAGTGGAAGAGATGCCTGAAGTTGTTGCTTAGGCGGTACAAGGTGGCTACTCTCAGTCTCGGTGGACATGAAATTTCAGCTCTCAAGAATTTTGATCCTTCCTCCCCATACAATATTATTTGTTTCTCTGACAACGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGATGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTCCAAGATTGCACGAGGATGAGAATTGGACAGGACTTGCTATATGTGTTACCTTTGAAGTTCATGACCAGCGTCCAGCTACCTCCCCAGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTTCCATCACTAAAGAGAAACTCAAGTCGTTGCATCTTGGTCGATTCATTTGGGTAACCTACATCCCCAGTATTTTTCTAACGGAGTTCAGTGTGATTAGTGATGTAGAGGCCAGAATTTATGTTGGTTGCAGAGGCTTGACAGTGGACAAGTGTGGTATGCGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAACACAATAACCGAATGCTGGACCTCTTTCTTCGATAATCTGTCTTTCATCCGTCAACTTGTAGAAGCAGATGATCAACCGCATGAACTTCCAATGCTTGAAGTGCGTACCAAGGTCTTTGATCCGGATTTAATATACAATGCAATCTGCCCTTCTAATGAAATTCTGGAGTGGTTCGGGCATCGAATTGAGGATTCCTCGGATGCCAGCTGGGGCTGCGAATTCCAGTTACCACCATCTCTGAGCGACACAAATTGGATAGGATTGTCCATCTGTGTATCATACCGTATTAGAAGAGTGGATTGGCACGAACCTAATTCCCACCCCTTTAATTTAACATTGAAAACCAGAAACAATGCTTTGTCGTCTCTGCATCGGTATCAAAGTAGCAATGAAGAATATCAGTTCGTAAACAGTTGTCGTATTATGAATCGTAGATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGATGAGTCTGTCCTCTTTGCTTCACTCCGGGACACCTGGTGGAGCCCGGCCAAGATCTATCACCGATTTGTATATGCGCAGGAAGAGTTCAAGCAGCTCTGTTTCAATCTCGATCGACCTGGTGCCCGTGCCCCACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

856

Amino Acids

98.48

Weight (kDa)

6.04

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 505 - 642 2.1e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2508
AccB1I GGYRCC 1 cut(s) 2552
AccII CGCG 1 cut(s) 178
AccIII TCCGGA 1 cut(s) 2065
AciI CCGC 3 cut(s) 176, 1420, 2021
AclWI GGATC 6 cut(s) 433, 768, 781, 958, 1475, 2057
AcoI YGGCCR 1 cut(s) 2478
AcuI CTGAAG 4 cut(s) 216, 1216, 1349, 1424
AcvI CACGTG 1 cut(s) 836
AdeI CACNNNGTG 1 cut(s) 2469
AfaI GTAC 9 cut(s) 380, 688, 831, 1111, 1122, 1341, 1424, 1916, 2050
AfiI CCNNNNNNNGG 4 cut(s) 182, 576, 598, 1959
AflIII ACRYGT 3 cut(s) 826, 835, 1003
AhlI ACTAGT 1 cut(s) 1117
AjiI CACGTC 1 cut(s) 524
AjnI CCWGG 3 cut(s) 575, 2465, 2548
AjuI GAANNNNNNNTTGG 2 cut(s) 2227, 2259
AleI CACNNNNGTG 1 cut(s) 1387
Alw26I GTCTC 7 cut(s) 65, 241, 389, 1349, 1448, 1913, 2313
AlwI GGATC 6 cut(s) 433, 768, 781, 958, 1475, 2057
Aor13HI TCCGGA 1 cut(s) 2065
AoxI GGCC 3 cut(s) 1029, 1854, 2478
ApeKI GCWGC 7 cut(s) 453, 456, 980, 1156, 1159, 2157, 2524
Asp700I GAANNNNTTC 1 cut(s) 533
AspLEI GCGC 3 cut(s) 881, 1050, 2509
AspS9I GGNCC 6 cut(s) 101, 801, 1030, 1225, 1279, 1962
AsuC2I CCSGG 3 cut(s) 1028, 2459, 2477
AsuHPI GGTGA 4 cut(s) 419, 581, 1374, 2483
AvaII GGWCC 5 cut(s) 101, 801, 1225, 1279, 1962
BaeGI GKGCMC 2 cut(s) 2557, 2563
BamHI GGATCC 1 cut(s) 773
BanI GGYRCC 1 cut(s) 2552
BanII GRGCYC 1 cut(s) 2477
BarI GAAGNNNNNNTAC 2 cut(s) 679, 711
BauI CACGAG 1 cut(s) 1607
BbrPI CACGTG 1 cut(s) 836
BbsI GAAGAC 3 cut(s) 303, 963, 1557
BbvI GCAGC 7 cut(s) 440, 468, 967, 1146, 1168, 2144, 2536
BccI CCATC 8 cut(s) 409, 594, 642, 901, 1082, 1757, 2185, 2219
BceAI ACGGC 1 cut(s) 915
BcgI CGANNNNNNTGC 2 cut(s) 1766, 1800
BciT130I CCWGG 3 cut(s) 577, 2467, 2550
BciVI GTATCC 1 cut(s) 270
BclI TGATCA 3 cut(s) 640, 748, 2014
BcnI CCSGG 3 cut(s) 1028, 2459, 2477
BcoDI GTCTC 7 cut(s) 65, 241, 389, 1349, 1448, 1913, 2313
BcuI ACTAGT 1 cut(s) 1117
BfaI CTAG 3 cut(s) 1118, 1569, 1575
BfmI CTRYAG 3 cut(s) 303, 454, 1375
BfuI GTATCC 1 cut(s) 270
BglII AGATCT 1 cut(s) 2484
BisI GCNGC 7 cut(s) 454, 457, 981, 1157, 1160, 2158, 2525
BlsI GCNGC 7 cut(s) 455, 458, 982, 1158, 1161, 2159, 2526
BmcAI AGTACT 2 cut(s) 1111, 1122
Bme1390I CCNGG 6 cut(s) 577, 1028, 2459, 2467, 2477, 2550
Bme18I GGWCC 5 cut(s) 101, 801, 1225, 1279, 1962
BmgBI CACGTC 1 cut(s) 524
BmgT120I GGNCC 6 cut(s) 101, 801, 1030, 1225, 1279, 1962
BmiI GGNNCC 5 cut(s) 775, 802, 1280, 2474, 2554
BmrFI CCNGG 6 cut(s) 577, 1028, 2459, 2467, 2477, 2550
BmrI ACTGGG 2 cut(s) 1676, 1806
BmsI GCATC 9 cut(s) 503, 889, 1091, 1163, 1388, 1786, 2131, 2134, 2323
BmuI ACTGGG 2 cut(s) 1676, 1806
BoxI GACNNNNGTC 1 cut(s) 1665
BpiI GAAGAC 3 cut(s) 303, 963, 1557
BplI GAGNNNNNCTC 2 cut(s) 2123, 2155
BpmI CTGGAG 3 cut(s) 1166, 1240, 2129
Bpu10I CCTNAGC 1 cut(s) 1415
BpuEI CTTGAG 4 cut(s) 764, 1454, 1723, 1751
BpuMI CCSGG 3 cut(s) 1028, 2459, 2477
BsaAI YACGTR 1 cut(s) 836
BsaI GGTCTC 1 cut(s) 241
BsaJI CCNNGG 5 cut(s) 575, 1026, 1710, 2052, 2139
BsaWI WCCGGW 1 cut(s) 2065
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 576, 598, 1959
Bse118I RCCGGY 1 cut(s) 1917
Bse1I ACTGG 6 cut(s) 409, 1219, 1529, 1682, 1812, 2167
Bse3DI GCAATG 5 cut(s) 370, 523, 1057, 1296, 2338
BseAI TCCGGA 1 cut(s) 2065
BseBI CCWGG 3 cut(s) 577, 2467, 2550
BseDI CCNNGG 5 cut(s) 575, 1026, 1710, 2052, 2139
BseGI GGATG 9 cut(s) 204, 424, 518, 607, 916, 1618, 1806, 1989, 2149
BseLI CCNNNNNNNGG 4 cut(s) 182, 576, 598, 1959
BseMI GCAATG 5 cut(s) 370, 523, 1057, 1296, 2338
BseMII CTCAG 4 cut(s) 819, 1344, 1453, 2174
BseNI ACTGG 6 cut(s) 409, 1219, 1529, 1682, 1812, 2167
BseRI GAGGAG 2 cut(s) 407, 501
BseSI GKGCMC 2 cut(s) 2557, 2563
BseXI GCAGC 7 cut(s) 440, 468, 967, 1146, 1168, 2144, 2536
BseYI CCCAGC 2 cut(s) 146, 2151
BsgI GTGCAG 2 cut(s) 1098, 1175
Bsh1236I CGCG 1 cut(s) 178
Bsh1285I CGRYCG 1 cut(s) 2545
BshFI GGCC 3 cut(s) 1031, 1856, 2480
BshNI GGYRCC 1 cut(s) 2552
BsiEI CGRYCG 1 cut(s) 2545
BsiSI CCGG 5 cut(s) 1028, 1918, 2066, 2458, 2477
BslFI GGGAC 4 cut(s) 18, 814, 1292, 2474
BslI CCNNNNNNNGG 4 cut(s) 182, 576, 598, 1959
BsmAI GTCTC 7 cut(s) 65, 241, 389, 1349, 1448, 1913, 2313
BsmBI CGTCTC 2 cut(s) 1913, 2313
BsmFI GGGAC 4 cut(s) 18, 814, 1292, 2474
BsmI GAATGC 1 cut(s) 1961
BsnI GGCC 3 cut(s) 1031, 1856, 2480
Bso31I GGTCTC 1 cut(s) 241
Bsp1286I GDGCHC 3 cut(s) 2477, 2557, 2563
Bsp13I TCCGGA 1 cut(s) 2065
Bsp1407I TGTACA 2 cut(s) 686, 1339
BspACI CCGC 3 cut(s) 176, 1420, 2021
BspANI GGCC 3 cut(s) 1031, 1856, 2480
BspCNI CTCAG 4 cut(s) 818, 1345, 1452, 2175
BspEI TCCGGA 1 cut(s) 2065
BspFNI CGCG 1 cut(s) 178
BspHI TCATGA 3 cut(s) 745, 751, 1657
BspLI GGNNCC 5 cut(s) 775, 802, 1280, 2474, 2554
BspMAI CTGCAG 2 cut(s) 307, 458
BspPI GGATC 6 cut(s) 433, 768, 781, 958, 1475, 2057
BspT107I GGYRCC 1 cut(s) 2552
BspTNI GGTCTC 1 cut(s) 241
BsrDI GCAATG 5 cut(s) 370, 523, 1057, 1296, 2338
BsrFI RCCGGY 1 cut(s) 1917
BsrGI TGTACA 2 cut(s) 686, 1339
BsrI ACTGG 6 cut(s) 409, 1219, 1529, 1682, 1812, 2167
BssAI RCCGGY 1 cut(s) 1917
BssECI CCNNGG 5 cut(s) 575, 1026, 1710, 2052, 2139
BssSI CACGAG 1 cut(s) 1607
BssT1I CCWWGG 2 cut(s) 1710, 2052
Bst2BI CACGAG 1 cut(s) 1607
Bst2UI CCWGG 3 cut(s) 577, 2467, 2550
Bst4CI ACNGT 6 cut(s) 791, 1389, 1888, 2228, 2357, 2568
Bst6I CTCTTC 6 cut(s) 63, 239, 1388, 1920, 2230, 2508
BstAUI TGTACA 2 cut(s) 686, 1339
BstBAI YACGTR 1 cut(s) 836
BstC8I GCNNGC 1 cut(s) 2149
BstDEI CTNAG 5 cut(s) 805, 1353, 1415, 1439, 2183
BstEII GGTNACC 2 cut(s) 407, 1798
BstF5I GGATG 9 cut(s) 204, 424, 518, 607, 916, 1618, 1806, 1989, 2149
BstFNI CGCG 1 cut(s) 178
BstHHI GCGC 3 cut(s) 881, 1050, 2509
BstMAI GTCTC 7 cut(s) 65, 241, 389, 1349, 1448, 1913, 2313
BstMCI CGRYCG 1 cut(s) 2545
BstMWI GCNNNNNNNGC 4 cut(s) 184, 1061, 2044, 2157
BstNI CCWGG 3 cut(s) 577, 2467, 2550
BstNSI RCATGY 2 cut(s) 830, 1007
BstPAI GACNNNNGTC 1 cut(s) 1665
BstPI GGTNACC 2 cut(s) 407, 1798
BstSCI CCNGG 6 cut(s) 575, 1026, 2457, 2465, 2475, 2548
BstSFI CTRYAG 3 cut(s) 303, 454, 1375
BstSLI GKGCMC 2 cut(s) 2557, 2563
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 7 cut(s) 440, 468, 967, 1146, 1168, 2144, 2536
BstV2I GAAGAC 3 cut(s) 303, 963, 1557
BstX2I RGATCY 3 cut(s) 425, 773, 2484
BstXI CCANNNNNNTGG 2 cut(s) 923, 2409
BstYI RGATCY 3 cut(s) 425, 773, 2484
BsuI GTATCC 1 cut(s) 270
BsuRI GGCC 3 cut(s) 1031, 1856, 2480
BtrI CACGTC 1 cut(s) 524
BtsCI GGATG 9 cut(s) 204, 424, 518, 607, 916, 1618, 1806, 1989, 2149
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 5 cut(s) 300, 1394, 1536, 1840, 1893
Cac8I GCNNGC 1 cut(s) 2149
CciI TCATGA 3 cut(s) 745, 751, 1657
CfoI GCGC 3 cut(s) 881, 1050, 2509
Cfr10I RCCGGY 1 cut(s) 1917
Cfr13I GGNCC 6 cut(s) 101, 801, 1030, 1225, 1279, 1962
CseI GACGC 3 cut(s) 1655, 1895, 2415
CsiI ACCWGGT 2 cut(s) 2465, 2548
Csp6I GTAC 9 cut(s) 379, 687, 830, 1110, 1121, 1340, 1423, 1915, 2049
CviQI GTAC 9 cut(s) 379, 687, 830, 1110, 1121, 1340, 1423, 1915, 2049
DdeI CTNAG 5 cut(s) 805, 1353, 1415, 1439, 2183
DraI TTTAAA 1 cut(s) 258
DraIII CACNNNGTG 1 cut(s) 2469
EaeI YGGCCR 1 cut(s) 2478
Eam1104I CTCTTC 6 cut(s) 63, 239, 1388, 1920, 2230, 2508
EarI CTCTTC 6 cut(s) 63, 239, 1388, 1920, 2230, 2508
Eco130I CCWWGG 2 cut(s) 1710, 2052
Eco24I GRGCYC 1 cut(s) 2477
Eco31I GGTCTC 1 cut(s) 241
Eco47I GGWCC 5 cut(s) 101, 801, 1225, 1279, 1962
Eco57I CTGAAG 4 cut(s) 216, 1216, 1349, 1424
Eco72I CACGTG 1 cut(s) 836
Eco91I GGTNACC 2 cut(s) 407, 1798
EcoO109I RGGNCCY 1 cut(s) 801
EcoO65I GGTNACC 2 cut(s) 407, 1798
EcoRI GAATTC 1 cut(s) 2162
EcoRII CCWGG 3 cut(s) 575, 2465, 2548
EcoT14I CCWWGG 2 cut(s) 1710, 2052
EcoT38I GRGCYC 1 cut(s) 2477
ErhI CCWWGG 2 cut(s) 1710, 2052
Esp3I CGTCTC 2 cut(s) 1913, 2313
FaqI GGGAC 4 cut(s) 18, 814, 1292, 2474
FauNDI CATATG 1 cut(s) 154
FbaI TGATCA 3 cut(s) 640, 748, 2014
Fnu4HI GCNGC 7 cut(s) 454, 457, 981, 1157, 1160, 2158, 2525
FokI GGATG 9 cut(s) 211, 431, 525, 614, 923, 1625, 1793, 1976, 2156
FriOI GRGCYC 1 cut(s) 2477
Fsp4HI GCNGC 7 cut(s) 454, 457, 981, 1157, 1160, 2158, 2525
FspBI CTAG 3 cut(s) 1118, 1569, 1575
FspI TGCGCA 1 cut(s) 2508
GlaI GCGC 3 cut(s) 880, 1049, 2508
GluI GCNGC 7 cut(s) 454, 457, 981, 1157, 1160, 2158, 2525
GsaI CCCAGC 2 cut(s) 150, 2155
GsuI CTGGAG 3 cut(s) 1166, 1240, 2129
HaeIII GGCC 3 cut(s) 1031, 1856, 2480
HapII CCGG 5 cut(s) 1028, 1918, 2066, 2458, 2477
HgaI GACGC 3 cut(s) 1655, 1895, 2415
HhaI GCGC 3 cut(s) 881, 1050, 2509
Hin6I GCGC 3 cut(s) 879, 1048, 2507
HinP1I GCGC 3 cut(s) 879, 1048, 2507
HincII GTYRAC 2 cut(s) 1216, 1997
HindII GTYRAC 2 cut(s) 1216, 1997
HpaI GTTAAC 1 cut(s) 1216
HpaII CCGG 5 cut(s) 1028, 1918, 2066, 2458, 2477
HphI GGTGA 4 cut(s) 419, 581, 1374, 2483
Hpy166II GTNNAC 8 cut(s) 687, 1216, 1228, 1382, 1451, 1891, 1997, 2353
Hpy8I GTNNAC 8 cut(s) 687, 1216, 1228, 1382, 1451, 1891, 1997, 2353
Hpy99I CGWCG 1 cut(s) 2409
HpyAV CCTTC 4 cut(s) 32, 1149, 1494, 2103
HpyCH4III ACNGT 6 cut(s) 791, 1389, 1888, 2228, 2357, 2568
HpyCH4IV ACGT 4 cut(s) 523, 835, 992, 1212
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 1061, 2044, 2157
HpyF3I CTNAG 5 cut(s) 805, 1353, 1415, 1439, 2183
HpySE526I ACGT 4 cut(s) 523, 835, 992, 1212
HspAI GCGC 3 cut(s) 879, 1048, 2507
Kpn2I TCCGGA 1 cut(s) 2065
Ksp22I TGATCA 3 cut(s) 640, 748, 2014
KspAI GTTAAC 1 cut(s) 1216
LmnI GCTCC 4 cut(s) 559, 1028, 1141, 2472
Lsp1109I GCAGC 7 cut(s) 440, 468, 967, 1146, 1168, 2144, 2536
LweI GCATC 9 cut(s) 503, 889, 1091, 1163, 1388, 1786, 2131, 2134, 2323
MabI ACCWGGT 2 cut(s) 2465, 2548
MaeI CTAG 3 cut(s) 1118, 1569, 1575
MaeII ACGT 4 cut(s) 523, 835, 992, 1212
MaeIII GTNAC 8 cut(s) 407, 604, 614, 1245, 1642, 1697, 1798, 2169
MfeI CAATTG 2 cut(s) 1078, 1149
MflI RGATCY 3 cut(s) 425, 773, 2484
MhlI GDGCHC 3 cut(s) 2477, 2557, 2563
MlyI GAGTC 3 cut(s) 1214, 1351, 2444
MroI TCCGGA 1 cut(s) 2065
MroXI GAANNNNTTC 1 cut(s) 533
MseI TTAA 7 cut(s) 257, 386, 974, 1215, 2072, 2271, 2276
MslI CAYNNNNRTG 6 cut(s) 93, 591, 750, 1335, 1387, 1611
MspA1I CMGCKG 2 cut(s) 1159, 2151
MspI CCGG 5 cut(s) 1028, 1918, 2066, 2458, 2477
MspR9I CCNGG 6 cut(s) 577, 1028, 2459, 2467, 2477, 2550
MunI CAATTG 2 cut(s) 1078, 1149
Mva1269I GAATGC 1 cut(s) 1961
MvaI CCWGG 3 cut(s) 577, 2467, 2550
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 4 cut(s) 184, 1061, 2044, 2157
NciI CCSGG 3 cut(s) 1028, 2459, 2477
NdeI CATATG 1 cut(s) 154
NlaIV GGNNCC 5 cut(s) 775, 802, 1280, 2474, 2554
NmuCI GTSAC 2 cut(s) 407, 1697
NsbI TGCGCA 1 cut(s) 2508
NspI RCATGY 2 cut(s) 830, 1007
OliI CACNNNNGTG 1 cut(s) 1387
PagI TCATGA 3 cut(s) 745, 751, 1657
PciI ACATGT 2 cut(s) 826, 1003
PctI GAATGC 1 cut(s) 1961
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 8 cut(s) 353, 859, 1584, 1729, 1788, 2135, 2371, 2379
PfoI TCCNGGA 1 cut(s) 2457
PkrI GCNGC 7 cut(s) 455, 458, 982, 1158, 1161, 2159, 2526
Ple19I CGATCG 1 cut(s) 2545
PleI GAGTC 3 cut(s) 1213, 1351, 2443
PmaCI CACGTG 1 cut(s) 836
PmlI CACGTG 1 cut(s) 836
PpsI GAGTC 3 cut(s) 1213, 1351, 2443
Ppu21I YACGTR 1 cut(s) 836
PpuMI RGGWCCY 1 cut(s) 801
PscI ACATGT 2 cut(s) 826, 1003
PshAI GACNNNNGTC 1 cut(s) 1665
Psp5II RGGWCCY 1 cut(s) 801
Psp6I CCWGG 3 cut(s) 575, 2465, 2548
PspCI CACGTG 1 cut(s) 836
PspEI GGTNACC 2 cut(s) 407, 1798
PspFI CCCAGC 2 cut(s) 146, 2151
PspGI CCWGG 3 cut(s) 575, 2465, 2548
PspN4I GGNNCC 5 cut(s) 775, 802, 1280, 2474, 2554
PspPI GGNCC 6 cut(s) 101, 801, 1030, 1225, 1279, 1962
PspPPI RGGWCCY 1 cut(s) 801
PsrI GAACNNNNNNTAC 2 cut(s) 822, 854
PstI CTGCAG 2 cut(s) 307, 458
PsuI RGATCY 3 cut(s) 425, 773, 2484
PvuI CGATCG 1 cut(s) 2545
PvuII CAGCTG 2 cut(s) 1159, 2151
RsaI GTAC 9 cut(s) 380, 688, 831, 1111, 1122, 1341, 1424, 1916, 2050
RsaNI GTAC 9 cut(s) 379, 687, 830, 1110, 1121, 1340, 1423, 1915, 2049
RseI CAYNNNNRTG 6 cut(s) 93, 591, 750, 1335, 1387, 1611
SaqAI TTAA 7 cut(s) 257, 386, 974, 1215, 2072, 2271, 2276
SatI GCNGC 7 cut(s) 454, 457, 981, 1157, 1160, 2158, 2525
Sau96I GGNCC 6 cut(s) 101, 801, 1030, 1225, 1279, 1962
ScaI AGTACT 2 cut(s) 1111, 1122
SchI GAGTC 3 cut(s) 1214, 1351, 2444
ScrFI CCNGG 6 cut(s) 577, 1028, 2459, 2467, 2477, 2550
SduI GDGCHC 3 cut(s) 2477, 2557, 2563
SexAI ACCWGGT 2 cut(s) 2465, 2548
SfaNI GCATC 9 cut(s) 503, 889, 1091, 1163, 1388, 1786, 2131, 2134, 2323
SfcI CTRYAG 3 cut(s) 303, 454, 1375
SinI GGWCC 5 cut(s) 101, 801, 1225, 1279, 1962
SmiMI CAYNNNNRTG 6 cut(s) 93, 591, 750, 1335, 1387, 1611
SmlI CTYRAG 4 cut(s) 779, 1469, 1702, 1766
SmoI CTYRAG 4 cut(s) 779, 1469, 1702, 1766
SpeI ACTAGT 1 cut(s) 1117
SsiI CCGC 3 cut(s) 176, 1420, 2021
SspI AATATT 1 cut(s) 1501
SspMI CTAG 3 cut(s) 1118, 1569, 1575
StyD4I CCNGG 6 cut(s) 575, 1026, 2457, 2465, 2475, 2548
StyI CCWWGG 2 cut(s) 1710, 2052
TaaI ACNGT 6 cut(s) 791, 1389, 1888, 2228, 2357, 2568
TaiI ACGT 4 cut(s) 526, 838, 995, 1215
TaqII GACCGA 1 cut(s) 1296
TatI WGTACW 4 cut(s) 686, 1109, 1120, 1339
TfiI GAWTC 8 cut(s) 353, 859, 1584, 1729, 1788, 2135, 2371, 2379
Tru1I TTAA 7 cut(s) 257, 386, 974, 1215, 2072, 2271, 2276
Tru9I TTAA 7 cut(s) 257, 386, 974, 1215, 2072, 2271, 2276
TscAI CASTG 5 cut(s) 307, 1394, 1536, 1840, 1893
TseFI GTSAC 2 cut(s) 407, 1697
TseI GCWGC 7 cut(s) 453, 456, 980, 1156, 1159, 2157, 2524
Tsp45I GTSAC 2 cut(s) 407, 1697
TspGWI ACGGA 3 cut(s) 786, 1841, 1982
TspRI CASTG 5 cut(s) 307, 1394, 1536, 1840, 1893
VpaK11BI GGWCC 5 cut(s) 101, 801, 1225, 1279, 1962
XceI RCATGY 2 cut(s) 830, 1007
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XmnI GAANNNNTTC 1 cut(s) 533
XspI CTAG 3 cut(s) 1118, 1569, 1575
ZrmI AGTACT 2 cut(s) 1111, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.