Rroxscaffold_2G00137070

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000307
Physical Location & Seq
Forward (+)
74803196 .. 74811529
8334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137070.1

Sequence Viewer

Length: 4116 bp
ATGAAATTTCCTATTGTTTATCCTGACAATTCCTCCCAAGTTTTCATTGGCTCCCATTTCCCTTCTGCGGAGTTTCCAGCTAGCAGAGAATCGGAACCTGAAACCGATTCCAAACCATCTGAAATAAGGAAGCAAACCGGAAACTACGGCGAGGCGTTTGCTAAGCATGAGACAGATTCCGAGGACAAGATGAGGAAGGTGAACAAGTGGAGGGAAGCTTTAAAGGAGATAGGCAGTCTCTCCGGATGGCATGTCACCAAGGACAAGGACAGAGGCAGCAGGTCGTGGCTTGTTGAGGATGCTCGTGATGTACTCACAGAAAATACTGGAACAATTGCTGTTGAAGGCTTATTCTTAAGCTTACCTGTGTCTGGAACAATTACTGTTGAAGGCTTATTCTTAAGCTTACCTGAAAAAGAAGAAATGTCATTGGATGATGACCCATTCTTAACTATGTGCAACTTACGATTGCTGAAGATTTATAATGTGAACTTTCAGGATGTGCACTTAAGATATGTGTCTAAAAATTTACGACTTTTAGAATGGCACGAATGCCCTCTAGAATCTCTGCCATCTAATTTTAAATCAGACAAGCTGGTTGAATTCAAGATGCCTAACAGCCGCATTAAGCAACCATGGAATGAAACGTTTCGTCTGGGAAATCTGGAAGAACTCGATCTTTCCTACTGCCAATACTTGAAGAAGATCCCTGGTTTGAATGGGGTTCCAAATCTGAAGAAATTGATCCTTAAAGATCTAGAGGGCCTGCCCTGCTTTATCAGCCTGAAATCTCTTGAAATTCTTATTCTTTCGGGATGTTCAAAGCTTAACGACTTTCCAGAGATCCAGGGAGATATGGAGAATTTTTCACAACTGCATTTAGATGGGACAGCGTTAAGGCGTCTATCATTACCGATGAAGCATTTGAAATGCCCTATTTCGATAAATCTAAAAGGCTGCAAGAACCTTTTGACTGTTCCAGTCCTATTAAGTCTGAAATCTCTCAATCTGTCGGGCTGCTCAGGTATATCCATATTGCCCCCTAACCTGGGAATCATGGCACATTTGGAGGAGCTTGATGCCTCTGAAACTTCTATAACAGGGGTACCCAAGTCCATTTCATATTTGCAGAAGCTTAAAGTGCTATCTTTTTGCGGATGCAAAGGATTGCAATTGCCTAGCTGGTTCTCGGGTTTAACGTCTTTGACATCATTAAATCTACGGAGGTGTGGTCTAGCAGAAGATGTCTTTGATAGCCTCTACGGCTTGTCCTCTTTGCAGAATTTGGATCTCAGTGCAAACAATCTTTTGATCATACCTAATGATATTGGTTGCTTATCCTCTCTGCAGCGATTGCATTTGAGTGAGAACAAATTAGTGAGTATACCGAATGAAATTGGTCACTTATCCTCTTTGCAGCTTTTAGATTTGAGTGACAACAACTTCATGAGTATACCTGAGAGCATCTCTCATCTTTCTAAGCTTACAGAACTTCGCTTGTTTAGATGTAGTAAGCTACAGTCCTTGCCAAACAATGTTCCGTTCAATCTAAAACATGTCCATGCACAAGAATGTCCTATGTTGAAGAGGAACGCAGATATTTTGACAATATGGGCATCCGGAAAAGGGTTCTCTTCTATAAATTGCACACAACCTGACCAAGATGACGATCAGCCAAGTCACATCTCAGTTCCTAAATACATTGTGGTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAGGTTTCTTTTCTTTTTTACAGGACCGAGTTTATGGTAAAATGCCATTTGAAATTCGTTTTCCACATAGTACAACAATTCCAAATCTGTGGAAACATTGGAGTAATGGCCCATCTGTAACAGTCCCACTTTCTGATGGTAACAGTACGTGTATGGGACTTGCTCTGTTTGTTGTTTTTGAAATCCTTCACCTTGAGAAAGGCCTTTTCAACAAGATTTGGGAATCGGAGGAGACTGTCTGTGAATTTCACACTGATGATGGTTGTGAAAATTCCGTAGTTTTTCAGAACTTCATAGACTTCACGGTTGGATCATATGCACTTTGTTACTATGAACCACGAGGCGTGCAATTTGGTGGGATGTTCGATAACCCAAGCTCACTTCGAGCTTCAATTTCAACGAAGAGACGGAATTTAAAAGTGAGAGGTTGTGGGATGCGTCTAATATCAGAGGAAGACGCTGCCAAGTTTGTAAAGAGTTTAGCAAGTCAGACTGCTACCCAACATCTTGATTCCAGTTTTGATCGACATTGCGAGTACATATTAGATGAGGAGACAGCAACTGGTGACCTGATGGAACTGGGATCTACTTCAACTTTCAATGCAAATAGCTGCAGCAAAAGTAACTCTAAGATCAAATTGAGAGGAGAGCTGTCGATACTCTATGAGGGACGCAATGGACGTGAGAAGAGTTTCCATTTTTGCTTTCCTGCTCCAGTAATTTCAACCCCGCCCTGGTTCCTCCATCACCATGCAGGGGATGTAACATTGTGTTACATCACTAAAAACTTACTTGATGATCAGAGATGGGTGGGATTAGAACTATATGTTCAATTTTCTCAGCGGACATCTACTTCAACATCTGGCAATAATAGTTTTTTCTTTTGTGTTGATTTGTGCTCTCATGATCATGAAAGTATAATAATGCACGGATCCCTCAAGATAAATAGTTGTGTTGGGATGTCAGATCAATTTGTTGTATTACATGTACCACGTGTTCATTTTCAACAACAGTTGAATCAATGTCAGGGCATTAGCGCATTGTTCAGAACCATTGATGCAGAAATGGAGGTCCTAGTGTGTGGAAGCCGTTTAGCTTTTGAGCATGATTTGAAAGACTTGATCCATTCATTAACTGCAGGTGCCGGCACGTTGGGGCAACATGTCCTCACTCAACCATGCTCCCAGGCCCAACCTGTTGATTGGCCCGATGCAGATGAAGCAGAAGTGCCCATCAATTGCTGTTCCTGGTTTCATAGATGCAGAAGTATTGCACTAGTACTACCTGAACAAGCTCCTTCTTCTTCAATTGTGCGGCTGCGAAATCATAGATGCTATCTCCAGCAATCGCGAGGTTTTGGTGAGTCTGACCTTACCCAATTGGTCCACTTCAGATCAGTAATGTTATTCCACTCCAGAAGCCTTTTGGAAAACAGGGATAGAAACCAAGAAAACGTTGCCAAAGAAGATAAATCGTTGGTGTTGGCTCGTCATCATGTACGTATAATGGCTGAGACTCTACTTCAGAGAAGCTATAGTTCACCACGGTGGAAGAGATGCCTTCACTTGTTGCTTCGACAGTCCAAGGTGGCTACTCTCAGTCTTGGTGGACACACAATTTCAGCTTTCCAGAATTTTGATCCTTCCTCCCCATACAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCGTATCAGACGTCTTCTAGGTCTAGGGTGGGAATCAAACTACCTTCAAGATTGCACGAGGATGAGAATTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGAACAGCGTCCAACTACCTCCCTGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTTCCATCACTGAAGAGAAACTCAAGTCGTTGCATCTTGGTCGATTCATTTGGGTAACCTACATCCCCAGTATTTTTCTATCGGAGTTCAGTGTGGTTAGTGATGTAGAGGCCAGAATATATGTTAGCTGCAGAGGCTTGACAGTGGAGAAATGCGGGATCCGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAATGCAATAACCGAGTGCTGGACCTCTTTCTTTGATAATCTGCCTTTCATCCGTCAACTTGTGGAAGCAGATGATCAAAATGTTCAACCTTGGACAAGGCATGAACTTCCGACGCTTGAAGGGCATATCAAGGTCTTTGATCCGGATTTAATATATAATGCAATCCCCCTTCTACTGGAATTCCGGAGTGGTTTGGCGCTGACATTAAGGACTGCTCAGCTACCGAGAGCTGGAGCTGGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1371

Amino Acids

154.6

Weight (kDa)

6.28

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 37 - 91 1.8e-07 TIR domain
LRR_14 PF23598 316 - 433 2.2e-11 Leucine-rich repeat region
LRR_14 PF23598 441 - 511 3.8e-10 Leucine-rich repeat region
LRR_8 PF13855 448 - 503 4.9e-08 Leucine rich repeat
C-JID PF20160 1148 - 1285 4e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
AarI CACCTGC 1 cut(s) 2900
AatII GACGTC 1 cut(s) 3494
Acc36I ACCTGC 2 cut(s) 270, 2900
Acc65I GGTACC 1 cut(s) 1105
AccB1I GGYRCC 2 cut(s) 1105, 2912
AccI GTMKAC 2 cut(s) 1384, 1453
AccII CGCG 1 cut(s) 3121
AccIII TCCGGA 4 cut(s) 242, 1619, 4012, 4053
AciI CCGC 7 cut(s) 68, 622, 1155, 2501, 2614, 3085, 3828
AclI AACGTT 2 cut(s) 647, 3225
AcuI CTGAAG 5 cut(s) 494, 755, 3145, 3278, 3705
AcvI CACGTG 1 cut(s) 2765
AcyI GRCGYC 2 cut(s) 901, 3491
AfaI GTAC 9 cut(s) 312, 1107, 1843, 1918, 2309, 2760, 3051, 3270, 3845
AflII CTTAAG 3 cut(s) 355, 400, 508
AflIII ACRYGT 5 cut(s) 1555, 1919, 2755, 2764, 2932
AhlI ACTAGT 1 cut(s) 3046
AjiI CACGTC 1 cut(s) 2453
AjnI CCWGG 7 cut(s) 709, 846, 1047, 2504, 2955, 3017, 3609
AleI CACNNNNGTG 1 cut(s) 3316
Alw21I GWGCWC 2 cut(s) 507, 2672
Alw26I GTCTC 8 cut(s) 164, 242, 1714, 1997, 2170, 2318, 3278, 3842
Alw44I GTGCAC 1 cut(s) 503
AlwNI CAGNNNCTG 1 cut(s) 2333
Ama87I CYCGRG 1 cut(s) 1189
Aor13HI TCCGGA 4 cut(s) 242, 1619, 4012, 4053
AoxI GGCC 6 cut(s) 763, 1879, 1972, 2958, 2975, 3783
ApaLI GTGCAC 1 cut(s) 503
Asp700I GAANNNNTTC 3 cut(s) 648, 1956, 2462
Asp718I GGTACC 1 cut(s) 1105
AspLEI GCGC 2 cut(s) 2810, 4069
AspS9I GGNCC 8 cut(s) 763, 1795, 1880, 2842, 2959, 2976, 3154, 3891
AsuHPI GGTGA 7 cut(s) 211, 247, 1952, 2348, 2510, 3143, 3303
AsuNHI GCTAGC 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 1189
AvaII GGWCC 4 cut(s) 1795, 2842, 3154, 3891
BaeGI GKGCMC 2 cut(s) 507, 3003
BaeI ACNNNNGTAYC 4 cut(s) 1089, 1097, 1122, 1130
BamHI GGATCC 2 cut(s) 2702, 3831
BanI GGYRCC 2 cut(s) 1105, 2912
BauI CACGAG 3 cut(s) 303, 2109, 3536
BbrPI CACGTG 1 cut(s) 2765
BbsI GAAGAC 2 cut(s) 2232, 3486
Bbv12I GWGCWC 2 cut(s) 507, 2672
BceAI ACGGC 3 cut(s) 163, 1279, 2844
BcgI CGANNNNNNTGC 4 cut(s) 140, 174, 3695, 3729
BciT130I CCWGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
BclI TGATCA 4 cut(s) 1311, 2569, 2677, 3943
BcoDI GTCTC 8 cut(s) 164, 242, 1714, 1997, 2170, 2318, 3278, 3842
BcuI ACTAGT 1 cut(s) 3046
BfaI CTAG 9 cut(s) 81, 560, 758, 1179, 1235, 2846, 3047, 3498, 3504
BfmI CTRYAG 6 cut(s) 1346, 1517, 2383, 2907, 3304, 3802
BfoI RGCGCY 1 cut(s) 4070
BfrI CTTAAG 3 cut(s) 355, 400, 508
BfuAI ACCTGC 2 cut(s) 270, 2900
BglI GCCNNNNNGGC 1 cut(s) 1263
BglII AGATCT 1 cut(s) 754
BlpI GCTNAGC 2 cut(s) 162, 4086
BmcAI AGTACT 1 cut(s) 3051
Bme1390I CCNGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
Bme18I GGWCC 4 cut(s) 1795, 2842, 3154, 3891
BmeT110I CYCGRG 1 cut(s) 1189
BmgBI CACGTC 1 cut(s) 2453
BmgT120I GGNCC 8 cut(s) 763, 1795, 1880, 2842, 2959, 2976, 3154, 3891
BmiI GGNNCC 8 cut(s) 52, 96, 726, 1107, 2510, 2704, 2914, 3833
BmrFI CCNGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
BmrI ACTGGG 2 cut(s) 2360, 3735
BmtI GCTAGC 1 cut(s) 84
BmuI ACTGGG 2 cut(s) 2360, 3735
BpiI GAAGAC 2 cut(s) 2232, 3486
BplI GAGNNNNNCTC 4 cut(s) 1451, 1483, 1453, 1485
BpmI CTGGAG 3 cut(s) 2469, 3095, 3169
Bpu10I CCTNAGC 1 cut(s) 1021
Bpu1102I GCTNAGC 2 cut(s) 162, 4086
BpuEI CTTGAG 4 cut(s) 1985, 2693, 3652, 3680
BsaAI YACGTR 3 cut(s) 1920, 2765, 3272
BsaBI GATNNNNATC 1 cut(s) 1668
BsaHI GRCGYC 2 cut(s) 901, 3491
BsaI GGTCTC 1 cut(s) 1714
BsaWI WCCGGW 5 cut(s) 137, 242, 1619, 4012, 4053
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 1216, 1246
Bse118I RCCGGY 2 cut(s) 2915, 3846
Bse1I ACTGG 9 cut(s) 331, 980, 2286, 2338, 2355, 2486, 3458, 3741, 4050
Bse3DI GCAATG 2 cut(s) 2299, 2452
Bse8I GATNNNNATC 1 cut(s) 1668
BseAI TCCGGA 4 cut(s) 242, 1619, 4012, 4053
BseBI CCWGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
BseJI GATNNNNATC 1 cut(s) 1668
BseMI GCAATG 2 cut(s) 2299, 2452
BseMII CTCAG 9 cut(s) 1035, 1306, 1449, 1701, 1785, 2624, 3273, 3382, 4100
BseNI ACTGG 9 cut(s) 331, 980, 2286, 2338, 2355, 2486, 3458, 3741, 4050
BseRI GAGGAG 4 cut(s) 1085, 2015, 2336, 2430
BseSI GKGCMC 2 cut(s) 507, 3003
BseYI CCCAGC 1 cut(s) 4106
Bsh1236I CGCG 1 cut(s) 3121
BshFI GGCC 6 cut(s) 765, 1881, 1974, 2960, 2977, 3785
BshNI GGYRCC 2 cut(s) 1105, 2912
BsiHKAI GWGCWC 2 cut(s) 507, 2672
BsiHKCI CYCGRG 1 cut(s) 1189
BsiSI CCGG 7 cut(s) 138, 243, 1620, 2916, 3847, 4013, 4054
BslFI GGGAC 4 cut(s) 901, 1880, 1941, 2454
BsmAI GTCTC 8 cut(s) 164, 242, 1714, 1997, 2170, 2318, 3278, 3842
BsmBI CGTCTC 2 cut(s) 2170, 3842
BsmFI GGGAC 4 cut(s) 901, 1880, 1941, 2454
BsmI GAATGC 2 cut(s) 557, 3877
BsnI GGCC 6 cut(s) 765, 1881, 1974, 2960, 2977, 3785
Bso31I GGTCTC 1 cut(s) 1714
BsoBI CYCGRG 1 cut(s) 1189
Bsp1286I GDGCHC 3 cut(s) 507, 2672, 3003
Bsp13I TCCGGA 4 cut(s) 242, 1619, 4012, 4053
Bsp1720I GCTNAGC 2 cut(s) 162, 4086
Bsp19I CCATGG 1 cut(s) 635
Bsp68I TCGCGA 1 cut(s) 3121
BspACI CCGC 7 cut(s) 68, 622, 1155, 2501, 2614, 3085, 3828
BspANI GGCC 6 cut(s) 765, 1881, 1974, 2960, 2977, 3785
BspCNI CTCAG 9 cut(s) 1034, 1305, 1450, 1700, 1784, 2623, 3274, 3381, 4099
BspEI TCCGGA 4 cut(s) 242, 1619, 4012, 4053
BspFNI CGCG 1 cut(s) 3121
BspHI TCATGA 4 cut(s) 1446, 2674, 2680, 3586
BspLI GGNNCC 8 cut(s) 52, 96, 726, 1107, 2510, 2704, 2914, 3833
BspMAI CTGCAG 4 cut(s) 1350, 2387, 2911, 3806
BspMI ACCTGC 2 cut(s) 270, 2900
BspOI GCTAGC 1 cut(s) 84
BspT107I GGYRCC 2 cut(s) 1105, 2912
BspTI CTTAAG 3 cut(s) 355, 400, 508
BspTNI GGTCTC 1 cut(s) 1714
BsrDI GCAATG 2 cut(s) 2299, 2452
BsrFI RCCGGY 2 cut(s) 2915, 3846
BsrI ACTGG 9 cut(s) 331, 980, 2286, 2338, 2355, 2486, 3458, 3741, 4050
BssAI RCCGGY 2 cut(s) 2915, 3846
BssNAI GTATAC 2 cut(s) 1385, 1454
BssNI GRCGYC 2 cut(s) 901, 3491
BssSI CACGAG 3 cut(s) 303, 2109, 3536
BssT1I CCWWGG 5 cut(s) 258, 635, 3354, 3639, 3959
Bst1107I GTATAC 2 cut(s) 1385, 1454
Bst2BI CACGAG 3 cut(s) 303, 2109, 3536
Bst2UI CCWGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
Bst6I CTCTTC 7 cut(s) 1580, 1639, 2168, 2453, 3317, 3681, 3849
BstACI GRCGYC 2 cut(s) 901, 3491
BstAFI CTTAAG 3 cut(s) 355, 400, 508
BstAPI GCANNNNNTGC 1 cut(s) 1354
BstBAI YACGTR 3 cut(s) 1920, 2765, 3272
BstC8I GCNNGC 4 cut(s) 82, 767, 2117, 2917
BstDSI CCRYGG 2 cut(s) 635, 3314
BstEII GGTNACC 2 cut(s) 2336, 3727
BstFNI CGCG 1 cut(s) 3121
BstH2I RGCGCY 1 cut(s) 4070
BstHHI GCGC 2 cut(s) 2810, 4069
BstMAI GTCTC 8 cut(s) 164, 242, 1714, 1997, 2170, 2318, 3278, 3842
BstMWI GCNNNNNNNGC 8 cut(s) 771, 780, 1141, 1263, 1354, 2990, 3807, 3991
BstNI CCWGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
BstNSI RCATGY 4 cut(s) 254, 1559, 2759, 2936
BstPI GGTNACC 2 cut(s) 2336, 3727
BstSCI CCNGG 7 cut(s) 709, 846, 1047, 2504, 2955, 3017, 3609
BstSFI CTRYAG 6 cut(s) 1346, 1517, 2383, 2907, 3304, 3802
BstSLI GKGCMC 2 cut(s) 507, 3003
BstSNI TACGTA 1 cut(s) 3272
BstUI CGCG 1 cut(s) 3121
BstV2I GAAGAC 2 cut(s) 2232, 3486
BstX2I RGATCY 7 cut(s) 705, 754, 843, 1288, 2354, 2702, 3831
BstXI CCANNNNNNTGG 1 cut(s) 1860
BstYI RGATCY 7 cut(s) 705, 754, 843, 1288, 2354, 2702, 3831
BstZ17I GTATAC 2 cut(s) 1385, 1454
BsuRI GGCC 6 cut(s) 765, 1881, 1974, 2960, 2977, 3785
BtgI CCRYGG 2 cut(s) 635, 3314
BtrI CACGTC 1 cut(s) 2453
BtsIMutI CAGTG 6 cut(s) 1300, 2022, 3465, 3681, 3769, 3822
BtuMI TCGCGA 1 cut(s) 3121
BveI ACCTGC 2 cut(s) 270, 2900
Cac8I GCNNGC 4 cut(s) 82, 767, 2117, 2917
CaiI CAGNNNCTG 1 cut(s) 2333
CciI TCATGA 4 cut(s) 1446, 2674, 2680, 3586
CfoI GCGC 2 cut(s) 2810, 4069
Cfr10I RCCGGY 2 cut(s) 2915, 3846
Cfr13I GGNCC 8 cut(s) 763, 1795, 1880, 2842, 2959, 2976, 3154, 3891
CseI GACGC 6 cut(s) 890, 2198, 2237, 2451, 3584, 3991
Csp6I GTAC 9 cut(s) 311, 1106, 1842, 1917, 2308, 2759, 3050, 3269, 3844
CviQI GTAC 9 cut(s) 311, 1106, 1842, 1917, 2308, 2759, 3050, 3269, 3844
DraI TTTAAA 3 cut(s) 222, 583, 2187
Eam1104I CTCTTC 7 cut(s) 1580, 1639, 2168, 2453, 3317, 3681, 3849
EarI CTCTTC 7 cut(s) 1580, 1639, 2168, 2453, 3317, 3681, 3849
Eco105I TACGTA 1 cut(s) 3272
Eco130I CCWWGG 5 cut(s) 258, 635, 3354, 3639, 3959
Eco147I AGGCCT 1 cut(s) 1974
Eco31I GGTCTC 1 cut(s) 1714
Eco47I GGWCC 4 cut(s) 1795, 2842, 3154, 3891
Eco57I CTGAAG 5 cut(s) 494, 755, 3145, 3278, 3705
Eco72I CACGTG 1 cut(s) 2765
Eco88I CYCGRG 1 cut(s) 1189
Eco91I GGTNACC 2 cut(s) 2336, 3727
EcoO109I RGGNCCY 2 cut(s) 763, 2842
EcoO65I GGTNACC 2 cut(s) 2336, 3727
EcoRI GAATTC 2 cut(s) 602, 4049
EcoRII CCWGG 7 cut(s) 709, 846, 1047, 2504, 2955, 3017, 3609
EcoT14I CCWWGG 5 cut(s) 258, 635, 3354, 3639, 3959
ErhI CCWWGG 5 cut(s) 258, 635, 3354, 3639, 3959
Esp3I CGTCTC 2 cut(s) 2170, 3842
FaqI GGGAC 4 cut(s) 901, 1880, 1941, 2454
FauI CCCGC 2 cut(s) 2508, 3821
FauNDI CATATG 1 cut(s) 2086
FbaI TGATCA 4 cut(s) 1311, 2569, 2677, 3943
FblI GTMKAC 2 cut(s) 1384, 1453
FspBI CTAG 9 cut(s) 81, 560, 758, 1179, 1235, 2846, 3047, 3498, 3504
GlaI GCGC 2 cut(s) 2809, 4068
GsaI CCCAGC 1 cut(s) 4110
GsuI CTGGAG 3 cut(s) 2469, 3095, 3169
HaeII RGCGCY 1 cut(s) 4070
HaeIII GGCC 6 cut(s) 765, 1881, 1974, 2960, 2977, 3785
HapII CCGG 7 cut(s) 138, 243, 1620, 2916, 3847, 4013, 4054
HgaI GACGC 6 cut(s) 890, 2198, 2237, 2451, 3584, 3991
HhaI GCGC 2 cut(s) 2810, 4069
Hin1I GRCGYC 2 cut(s) 901, 3491
Hin6I GCGC 2 cut(s) 2808, 4067
HinP1I GCGC 2 cut(s) 2808, 4067
HincII GTYRAC 1 cut(s) 3926
HindII GTYRAC 1 cut(s) 3926
HindIII AAGCTT 6 cut(s) 216, 358, 403, 824, 1133, 1481
HpaII CCGG 7 cut(s) 138, 243, 1620, 2916, 3847, 4013, 4054
HphI GGTGA 7 cut(s) 211, 247, 1952, 2348, 2510, 3143, 3303
Hpy166II GTNNAC 9 cut(s) 202, 490, 505, 1385, 1454, 3157, 3311, 3380, 3926
Hpy8I GTNNAC 9 cut(s) 202, 490, 505, 1385, 1454, 3157, 3311, 3380, 3926
Hpy99I CGWCG 1 cut(s) 3985
HpyCH4IV ACGT 9 cut(s) 647, 1199, 1919, 2452, 2764, 2921, 3225, 3271, 3491
HpyF10VI GCNNNNNNNGC 8 cut(s) 771, 780, 1141, 1263, 1354, 2990, 3807, 3991
HpySE526I ACGT 9 cut(s) 647, 1199, 1919, 2452, 2764, 2921, 3225, 3271, 3491
Hsp92I GRCGYC 2 cut(s) 901, 3491
HspAI GCGC 2 cut(s) 2808, 4067
Kpn2I TCCGGA 4 cut(s) 242, 1619, 4012, 4053
KpnI GGTACC 1 cut(s) 1109
KroI GCCGGC 1 cut(s) 2915
KroNI GCCGGC 1 cut(s) 2917
Ksp22I TGATCA 4 cut(s) 1311, 2569, 2677, 3943
LmnI GCTCC 6 cut(s) 56, 1072, 2488, 2957, 3070, 4103
MaeI CTAG 9 cut(s) 81, 560, 758, 1179, 1235, 2846, 3047, 3498, 3504
MaeII ACGT 9 cut(s) 647, 1199, 1919, 2452, 2764, 2921, 3225, 3271, 3491
MfeI CAATTG 5 cut(s) 333, 1172, 3007, 3078, 3149
MflI RGATCY 7 cut(s) 705, 754, 843, 1288, 2354, 2702, 3831
MhlI GDGCHC 3 cut(s) 507, 2672, 3003
MlyI GAGTC 2 cut(s) 3143, 3280
MmeI TCCRAC 3 cut(s) 2059, 3623, 4004
MroI TCCGGA 4 cut(s) 242, 1619, 4012, 4053
MroNI GCCGGC 1 cut(s) 2915
MroXI GAANNNNTTC 3 cut(s) 648, 1956, 2462
MslI CAYNNNNRTG 9 cut(s) 882, 1362, 1560, 1570, 2025, 2520, 2679, 3316, 3540
MspA1I CMGCKG 1 cut(s) 2614
MspCI CTTAAG 3 cut(s) 355, 400, 508
MspI CCGG 7 cut(s) 138, 243, 1620, 2916, 3847, 4013, 4054
MspR9I CCNGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
MunI CAATTG 5 cut(s) 333, 1172, 3007, 3078, 3149
Mva1269I GAATGC 2 cut(s) 557, 3877
MvaI CCWGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
MvnI CGCG 1 cut(s) 3121
MwoI GCNNNNNNNGC 8 cut(s) 771, 780, 1141, 1263, 1354, 2990, 3807, 3991
NaeI GCCGGC 1 cut(s) 2917
NcoI CCATGG 1 cut(s) 635
NdeI CATATG 1 cut(s) 2086
NgoMIV GCCGGC 1 cut(s) 2915
NheI GCTAGC 1 cut(s) 80
NlaIV GGNNCC 8 cut(s) 52, 96, 726, 1107, 2510, 2704, 2914, 3833
NmuCI GTSAC 6 cut(s) 253, 1400, 1433, 1679, 2336, 3626
NruI TCGCGA 1 cut(s) 3121
NspI RCATGY 4 cut(s) 254, 1559, 2759, 2936
OliI CACNNNNGTG 1 cut(s) 3316
PagI TCATGA 4 cut(s) 1446, 2674, 2680, 3586
PaqCI CACCTGC 1 cut(s) 2900
PceI AGGCCT 1 cut(s) 1974
PciI ACATGT 3 cut(s) 1555, 2755, 2932
PcsI WCGNNNNNNNCGW 3 cut(s) 1196, 2449, 3488
PctI GAATGC 2 cut(s) 557, 3877
PdiI GCCGGC 1 cut(s) 2917
PdmI GAANNNNTTC 3 cut(s) 648, 1956, 2462
PleI GAGTC 2 cut(s) 3142, 3280
PmaCI CACGTG 1 cut(s) 2765
PmlI CACGTG 1 cut(s) 2765
PpsI GAGTC 2 cut(s) 3142, 3280
Ppu21I YACGTR 3 cut(s) 1920, 2765, 3272
PpuMI RGGWCCY 1 cut(s) 2842
PscI ACATGT 3 cut(s) 1555, 2755, 2932
PsiI TTATAA 1 cut(s) 483
Psp1406I AACGTT 2 cut(s) 647, 3225
Psp5II RGGWCCY 1 cut(s) 2842
Psp6I CCWGG 7 cut(s) 709, 846, 1047, 2504, 2955, 3017, 3609
PspCI CACGTG 1 cut(s) 2765
PspEI GGTNACC 2 cut(s) 2336, 3727
PspFI CCCAGC 1 cut(s) 4106
PspGI CCWGG 7 cut(s) 709, 846, 1047, 2504, 2955, 3017, 3609
PspN4I GGNNCC 8 cut(s) 52, 96, 726, 1107, 2510, 2704, 2914, 3833
PspPI GGNCC 8 cut(s) 763, 1795, 1880, 2842, 2959, 2976, 3154, 3891
PspPPI RGGWCCY 1 cut(s) 2842
PsrI GAACNNNNNNTAC 2 cut(s) 2751, 2783
PstI CTGCAG 4 cut(s) 1350, 2387, 2911, 3806
PstNI CAGNNNCTG 1 cut(s) 2333
PsuI RGATCY 7 cut(s) 705, 754, 843, 1288, 2354, 2702, 3831
RruI TCGCGA 1 cut(s) 3121
RsaI GTAC 9 cut(s) 312, 1107, 1843, 1918, 2309, 2760, 3051, 3270, 3845
RsaNI GTAC 9 cut(s) 311, 1106, 1842, 1917, 2308, 2759, 3050, 3269, 3844
RseI CAYNNNNRTG 9 cut(s) 882, 1362, 1560, 1570, 2025, 2520, 2679, 3316, 3540
Sau96I GGNCC 8 cut(s) 763, 1795, 1880, 2842, 2959, 2976, 3154, 3891
ScaI AGTACT 1 cut(s) 3051
SchI GAGTC 2 cut(s) 3143, 3280
ScrFI CCNGG 7 cut(s) 711, 848, 1049, 2506, 2957, 3019, 3611
SduI GDGCHC 3 cut(s) 507, 2672, 3003
SfcI CTRYAG 6 cut(s) 1346, 1517, 2383, 2907, 3304, 3802
SinI GGWCC 4 cut(s) 1795, 2842, 3154, 3891
SmiMI CAYNNNNRTG 9 cut(s) 882, 1362, 1560, 1570, 2025, 2520, 2679, 3316, 3540
SmlI CTYRAG 7 cut(s) 355, 400, 508, 1964, 2708, 3631, 3695
SmoI CTYRAG 7 cut(s) 355, 400, 508, 1964, 2708, 3631, 3695
SnaBI TACGTA 1 cut(s) 3272
SpeI ACTAGT 1 cut(s) 3046
SseBI AGGCCT 1 cut(s) 1974
SsiI CCGC 7 cut(s) 68, 622, 1155, 2501, 2614, 3085, 3828
SspI AATATT 1 cut(s) 3430
SspMI CTAG 9 cut(s) 81, 560, 758, 1179, 1235, 2846, 3047, 3498, 3504
StuI AGGCCT 1 cut(s) 1974
StyD4I CCNGG 7 cut(s) 709, 846, 1047, 2504, 2955, 3017, 3609
StyI CCWWGG 5 cut(s) 258, 635, 3354, 3639, 3959
TaiI ACGT 9 cut(s) 650, 1202, 1922, 2455, 2767, 2924, 3228, 3274, 3494
TaqI TCGA 8 cut(s) 675, 941, 2136, 2155, 2296, 2426, 3346, 3715
TaqII GACCGA 1 cut(s) 1812
TatI WGTACW 4 cut(s) 310, 1841, 2307, 3049
TauI GCSGC 2 cut(s) 624, 3088
TscAI CASTG 6 cut(s) 1300, 2029, 3465, 3688, 3769, 3822
TseFI GTSAC 6 cut(s) 253, 1400, 1433, 1679, 2336, 3626
Tsp45I GTSAC 6 cut(s) 253, 1400, 1433, 1679, 2336, 3626
TspGWI ACGGA 7 cut(s) 1237, 1530, 2035, 2194, 2715, 3824, 3911
TspRI CASTG 6 cut(s) 1300, 2029, 3465, 3688, 3769, 3822
Vha464I CTTAAG 3 cut(s) 355, 400, 508
VneI GTGCAC 1 cut(s) 503
VpaK11BI GGWCC 4 cut(s) 1795, 2842, 3154, 3891
XbaI TCTAGA 2 cut(s) 559, 757
XceI RCATGY 4 cut(s) 254, 1559, 2759, 2936
XcmI CCANNNNNNNNNTGG 2 cut(s) 44, 3193
XmiI GTMKAC 2 cut(s) 1384, 1453
XmnI GAANNNNTTC 3 cut(s) 648, 1956, 2462
XspI CTAG 9 cut(s) 81, 560, 758, 1179, 1235, 2846, 3047, 3498, 3504
ZraI GACGTC 1 cut(s) 3492
ZrmI AGTACT 1 cut(s) 3051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.