Rw6G001370

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000307
Physical Location & Seq
Reverse (-)
2080298 .. 2097353
17056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001370.1

Sequence Viewer

Length: 6261 bp
ATGTTTACCGTCCTCCTTTTCCTCGTTCTCTTTGTTTCTCTTCTTCTTCAGTTTCTTAATTATCTTCTACCACCACCTGCCCAATCTCCAGAAGATTCCCCGAACATCCAAACACCTGCATCTCCTTCAAACCCTAAGTCAGAACATAAGGAAGTTCCAGCGGATTCCACAAACATCCAAATACCTGCATCTCCTTCGAACCCTAAATCAGAACATGATGAAGTTCCAGCGGCTTCCACAAACATCCAAATACTTGCATCTCCTTCAAACCCTAAATCAGAACATGAGGAAGTTCCAGCGGATTCCCCGAACATCCAAATACCTGCATCTCCTTCAACCCCTAAATTAGAACATGAGGAAGTTCCAGCAGATTCCACAAACACCCAAACAGTACTTTCATCTCTTTCAGCCCCAATGGAACATGAGAAAGTTCCTCCAGCAGCTTCCTCTTCTTCGGGCTCAACATTGGAGCATGATGTTGTATCCCATGATGTCTTCCTCAGTTTTAGAGGTGAAGACACCCGCTATGCTTTTACTGACCATTTATATCATGCTATGAGGCGGCAAGGAATTAATGCATTCAGAGATACAGAAAAACTCGAGAGGGGACATTCAATTTCACCCGATCTCTTGAAAGCAATAAAAGAATCCCGAATTGCAGTCATCATTCTTTCAGCTAACTATGCTACTTCAACATGGTGTTTGGAGGAGCTTGCACAGATTATTGAATGCAAAGAGTCGACGGGGTTGAAAGTTTTACCAGTTTTCTATCACGTCGAACCATCAGAAGTAAGGAAGCAAACAGGAAATTTTCACCAGGCGTTTTGCAAGCATGAAGAAACTTTCGAAGACAACCCGGAGAAGGTGAAGAAGTGGAGAGAAGCTTTAAAACAAGTAGCCAATCTCTCCGGATGGACTTTGAAAGAAAGAGGAGAATCTGAAGTTATTCAGACAATAACTACAGAGCTATTGAACACGTTGAATAACATGTGCTCAAGTGATAACAATGATTTAATTGGAATGGATGCTCGTATACAGAAACTAATGTCTTACTTGGATCTGGGGTCGAATGATGTTCGCCCCATAGGGATTGCCGGGATGGGCGGCATCGGAAAATCGACTCTGGCAAAGGCAGTTTTTGAAAAGATCCGGAAATCCCTAGTTAAATTGCAGAAACTTCTCTGTCAAAATTTGCAGCTGGACAACCATGAAAGTATACAGAATGATTCTATGGGGATCAATTTATTAAGGAAACGATTAAGTCATAAAAGGGTGCTTATCATTCTAGATGATGTTGATCATACTAGTCAACTAGAAGCTCTCGTTGCCAAAGAAGACAAAGGAAAAAAGCCTTGTTTGGGTCCAGGGAGCAGAGTCATTATAACAACTAGAGATACACACAAGCTGAAGGAATTTGGATTACTGGAAGATGCTATATATGAGGCTGAGAAGTTGACTGATAAGGAAGCTCTTCAGCTTTATGTCAGAAAGCTTTCAAGGAAAACCACTTTTGTAAAATATTGCAGTGGCCTTCCTTTAGCTCTCAAAGTTCTGGGTTCTCACTTGTGTACGAGAACGGTTGATGAATGGTCAGCAGAATTGGCTAGACTTGATGAAGATCCCCACAAAGATATTTTTTCTATTCTTCAATTAAGTTTTGATGGATTGAAGAATACAGAGAAAGATATCTTTTTGGACATAGCATGTTTCTTCAGAGGCGAGAGTCAATATCGTGTAAGAAAGATTTTAGAGAGCTGTGACTTTCATCCTGGTATCGGAATATCTGTCCTCGTTGAAAAACATTTAATTAATGTTGAGGAAGATAAACTGTGGATGCATGATTTGCTACAGCAAATGGGTTGGCACATTGTTCGGCAAGAATCTCCTGAGCCGGGAAAACGAAGCAGGTTGTGGCTTAATGAGAATTCCCATGAGTATCAACACAGCCGCCCGTGGTTTGATGAAGATACTCGTGATGTAGTCACCGAGAATCTGGGAACATATGCTGTCCAAGGGGTATATTTGAGCGTGCTGGAAAAAGAAAAGATACAATTGAAAGCTGACCCCTTCTTAAATATGCGCAAGCTAAGATTGTTGAAGATTTGTAATGTCAACTTGGTGGATGTGCGATTTGAATATCTGTCTAACAAGTTACGACTTTTGGAATGGCATGAATGTCCTCTAGAATCTCTGCCATCCAGTTTTCGACCAGATAAACTCGTCGAATTGAAACTGCCCAACAGCCGCATTGAAACACTGTGGAATCAAAGACTCTGTCTGGAGAAGCTAATACTGATGGATCTTTCCTACTGCCAATACTTGGTCAAGACCCCGGACTTCAGTAATTTCCCAAATCTTGAATTGTTGACTTTTGCAGGTTGTGAAAATTTATCAGATAGCCTTCCCAACTCCATCAGCTTGCAATCTCTTGCAATTCTTGTTCTTTCAGGATGTTCAAAACTAGATGAGTTTCCGGAGATAGTGGGAAACAGGGAAACTCTGGCAGCACTCTATTTAGATGGAACAGCTATAAGAGAGCTGCCGACAACAATCGGGTGCTTGAGTGGCCTAATTAGGTTAAATCTATGTGCCTGCAAGAACCTTGTGGGTCTTCCAAGCTTCATTTGCAGTTTGAAGTCTCTGAGATATCTGCGTCTGTCATATTCCTCACGCATTGACCAACTTCCAGAAGACATAGGGAGCTTGGAAAATTTGGAGAAGCTTGATGCTTGTTATACTGCTATAAGAAAAGTGCCTTCGTCCATTGTACTTCTGAAGAATCTGAAATTATTATGTTTCCATGGATGTAGGCAGGTGCCCGGGGAATGCTCTGATGCATCCTTGGGTTTGCAATTGCCTAAGTCGTTCGCCGGTTTGAGCTCATTGGAAACACTATCTTTAGGTCAATGCGATCTTGCTGAAGGAGCGATCCCTGATGACATTGGCTGCTTATCCTCACTGAAAGTTTTAAGGTTGGATGAAAACAATTTTGTGAGTATACCTGAAAGCATCTCTCAACTTCCTAACCTTAGAGAAATTCACATCCCAAATTGTAGCAAGCTTCAGTCTTTGCCAAAACGTCTTCCAAAGGATGTTAATGTTGAGGCTAGTGGATGTCCCCTGCAGACAAACTATCCAAACCAGCTTAACATATGGCTTTCTGAGGAGGGGATCCGTCACATAAATTGTGGAAATTCAAAGCATGCAAGCTGTCCACTTCCAATTCCCGAAGAACAAATTGAAGAATACTTACCCAAGTACCTTGAGGATGAAATTATACTTCAGCAGCACCTGCAGCTTCGTTACTTTCATACAGAAATTCCTGATTGGTGTCGTCACAAGAGTAGTGGGTCTTCTGTAGTAGCCATGCAAGTCTCTGATCCTGAAATTATTAAGAGCTTGGGAGTAGCCCTGTTTGTTGTTTTTGAAATCTTTGAGAGTGGTCACAATTTTGATAAGAGTTGGGAGTTGCAACAGACCCTTTGTACTTTTAAAGATGACCAAGGACTTGAACTTATCTATATCTTTAAAGAATTTGAAAAATTCCGGGCTGGAACACATGTATTATTTTGCTATGAGCCGCTAACGTACTTTCAAGTTGCTGGAATGTTGAATAAAGCAAGTCGACTTGAGGCTTCAGTTTCAACACAGAGACCAGATCTGGCGATTAAGTCTTGTGGGATGCAACTGATCTTTCAGGAAGAAGCTAAAGAGTTTGCTCAAAAATTAAGTGAGGCTTGTAATCCTCAGACGGATTTCGAATTTGGTAGACATTGTAAGGAATTGATAAAGGCCAAGGGAAAGAAAAGCTCCGACCACGAGAATCAAACTACCTCCATAGACAAAGTGAATCCAGAAATTGCTGAGCTTGTTTGTCATTTGGAAACTGAGAACAGTGGTTTCGAACATCTTCACCACTATCGACCAACCAATGAAGAACTCAAGCGGTTATGTCCTACAGAATACATTTGGCTATCTTATATACCACGTCAGGGGTATCTAGATCAGTTAAATGAGTGTGACCTCTTGGAGGCTTCATTTGCCAGCGAAAGCCGAGGATTGTTTGCTGAAGAATGTGGTCTCCGTCTTGTCTACCAGCATGATGAGCAAGAGTTTAAGAATCTGTGTACCTTCATGAACTCGTTTCTGGACAAAAGATCTGAAAGGTTGGAAGAATATGAGGCCCCCAAGAAGGGTAAACACGTTGTAGAATCTGATTTCAACATGCACCCGAGAGAAGATCTACAGTTGCTGCTTTCAGTGCTCTATAAGGGACCATGTGGACATGAAAAGGACTTCGGCCTTTTTTTTCCTCACAAGGTAGTACCTTTGTGGTTCTTCCATCACTATGCAGGGGACGTGGCACTGTGCGACTTTCCTCTAGACCTATCTGATGATCAGAGATGGGTCGGTTTAGAACTATTTGTTATGGTTTCGAGTGGTTACAGTATCAATGCTGAATCCTTACTTGATGTTGATTTGTGTTCCGATAAAATTAGCACAATAAGCACATCTGTAAAGATAAACACTTCTCTGGGCTCAGATCAACTTGTTGTAATCCATATACCACGCGTGCGTTTTCCGGAGCAGTTAAATCAATGTGACGGTATCAGCGCATTGTTTAGAACCACTACTGCAGATATGAGGGTTCAAATGTGTGGAAGCCGTTTACTATACGAGCAAGATTTGGAAGACTTGATCCGTGCAATATCCGTGTGCACATTAGGCAGTCCAGATGTCATTGATTCACTTTGTCAGCACATCCAACAATTACTTGGAAGTTGCATGGCGGTGGAACCTCTTGATCATATCGCAGCAGTTGAAACCGCTGAGCAGAATTCAGTGGAAGGAGAAAAGGACGTTGGTCGCAAAAATGTTCATTGCGCCACTGAAAGGGAGCACTTGATTCAAGACCGGAGAAAGCAAGTCTTCACTTCAATATTGCACAACTACAGATCTTATCTCCAACAAAAGCATGGTTGTATAGAGTCCGAAGGAATTATATCCCATTCCGAAGGCTATATTTTGGACAATGGGAATCCAAAGCCGGTGTTTGCCACGCATCATTTACACGTGATGGTTGAGACTCGACTCTATGGGAGCCTTAGCTCAGAAGATTGGAAACGATCTCTCCAGTCATTGCTTCAGTATTTCAAGGTGGCCACTCTCACTGTCAGAGGACAAATGATTGCTGTTTTAAATCCGTTCACTCCTTCCTCAGTAAACAACTTTTGTTTCCCACGAAAGGAACTTCCAGGATGGTTCTGGCGACTGAGAGTGTCCAGGATTAGATTGAACATACACCCACTTTTATGTGATGATGAGAATTGGTTGGGACTCGCTATATGTGCTACCTTTTCAGTCCACGAGCATCCAAGTGCTGTCCTTGACAATCTGGCCACAAGGACTTTCAACGTTATGTGTCATTTGGCAGCGTTGGATGGACGATGTTTGAGTCATGCCCCCGTGTTTACCATAACAAAAGAGAAATTCAAGTGGCTACATCTTCGAGGATTTATGTGGTTAACCTACATACCTCGTTCTTGTCTTCCAGAGTTAAAAGAAGTAGGTGTTGCAGAAGCTAGAATTTATAATGATTGCCCAGGATTAATGATGAAAGAGTGGAGTATCCGTCCCTTGTTCAAGCAAGATATGAAGGAATTTAATAAAGCAATAGTCCACTGCTGGACTTCGTTCTTCGACGACAATTTGGATCTCATCCGTCAGTCTGTCGAAGCAGATGAAAGAGAACAGGCACATTATTTTGACAGAGGGGAAACCAACCTCGAGAATCAAACTACCTCCATAGACAAAGTGAATCCAGAAATTGCTGAGCTTGTTTGTCATTTGGAAACTGAGAACGGTAGTTTCAAACATCTTCACCACTATCGACCAACCAATGAAGAATTCAAGCGGTTATGTCCTACAGATTTCATTTGGCTATCTTATCTACCACGTCAGGGGTATCTAGATCAGTTAAATAATGAGTGTGACCTCTTGGAGGCTTCATTTGCCAGTGAAAGCCGACGGGGGTTTGCTGAAGAATGTGGCCTCCGTCTTATCTACCAGCATGATGAGCAAGAGTTTAAGGATCTGTGTATCTTCATGAACTCGTTGCTGGACAAAAAATCAGAAAGATTGGAAGAACATGAGCCTCCCAATAAGGGTAAACAAGTTCTAGAATACCCTGAACCTGAGGTTAAATGGGGATCTAATGCAATGTGGAATCTAGAGATCTACGACCCAAAAACGAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

2086

Amino Acids

237.5

Weight (kDa)

5.54

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 163 - 337 7.9e-56 TIR domain
TIR_2 PF13676 165 - 275 6.8e-16 TIR domain
NB-ARC PF00931 388 - 499 2.1e-06 NB-ARC domain
WHD_ROQ1 PF23282 559 - 628 5.2e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 776 - 881 1.1e-08 Leucine-rich repeat region
LRR_13 PF23286 807 - 880 7.5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 873 - 969 5.1e-15 Leucine-rich repeat region
LRR_13 PF23286 890 - 1027 1.3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1382, 5592
AarI CACCTGC 4 cut(s) 85, 124, 2803, 3300
Acc16I TGCGCA 1 cut(s) 2081
Acc36I ACCTGC 8 cut(s) 85, 124, 193, 331, 1896, 2366, 2803, 3300
AccB1I GGYRCC 1 cut(s) 2815
AccB7I CCANNNNNTGG 1 cut(s) 2320
AccI GTMKAC 7 cut(s) 740, 1033, 1216, 2998, 3625, 3769, 4092
AccII CGCG 1 cut(s) 4572
AccIII TCCGGA 4 cut(s) 908, 1149, 2473, 4582
AclI AACGTT 1 cut(s) 5415
AcoI YGGCCR 2 cut(s) 5160, 5397
AcvI CACGTG 1 cut(s) 5074
AfaI GTAC 8 cut(s) 393, 1570, 2769, 3260, 3487, 3590, 4129, 4326
AflIII ACRYGT 6 cut(s) 975, 987, 3559, 4201, 4570, 5071
AhlI ACTAGT 1 cut(s) 1304
AjiI CACGTC 4 cut(s) 775, 3989, 4360, 5957
AjnI CCWGG 6 cut(s) 816, 1363, 1768, 5254, 5282, 5602
AjuI GAANNNNNNNTTGG 4 cut(s) 4845, 4877, 5891, 5923
Alw21I GWGCWC 5 cut(s) 995, 2882, 4266, 4721, 4902
Alw26I GTCTC 5 cut(s) 2643, 3379, 3646, 4085, 5078
Alw44I GTGCAC 1 cut(s) 4717
AlwNI CAGNNNCTG 2 cut(s) 3292, 4252
Ama87I CYCGRG 4 cut(s) 599, 2819, 4231, 5786
Aor13HI TCCGGA 4 cut(s) 908, 1149, 2473, 4582
AoxI GGCC 8 cut(s) 1528, 2566, 3792, 4182, 4300, 5160, 5397, 6049
ApaLI GTGCAC 1 cut(s) 4717
ArsI GACNNNNNNTTYG 4 cut(s) 122, 154, 2260, 2292
AseI ATTAAT 3 cut(s) 573, 1809, 5609
Asp700I GAANNNNTTC 4 cut(s) 945, 1470, 1492, 3909
AspLEI GCGC 3 cut(s) 2082, 4616, 4886
AspS9I GGNCC 3 cut(s) 1361, 4183, 4274
AsuC2I CCSGG 7 cut(s) 857, 1096, 1893, 2333, 2820, 2821, 3548
AsuHPI GGTGA 7 cut(s) 524, 612, 806, 877, 1975, 3905, 5873
AsuII TTCGAA 4 cut(s) 197, 846, 3759, 3903
AvaI CYCGRG 4 cut(s) 599, 2819, 4231, 5786
AvaII GGWCC 2 cut(s) 1361, 4274
AxyI CCTNAGG 1 cut(s) 6195
BaeGI GKGCMC 2 cut(s) 2820, 4721
BaeI ACNNNNGTAYC 2 cut(s) 3242, 3275
BalI TGGCCA 2 cut(s) 5162, 5399
BamHI GGATCC 1 cut(s) 3171
BanI GGYRCC 1 cut(s) 2815
BanII GRGCYC 3 cut(s) 461, 2882, 4541
BarI GAAGNNNNNNTAC 4 cut(s) 3234, 3266, 3337, 3369
BauI CACGAG 3 cut(s) 1971, 3818, 5366
BbrPI CACGTG 1 cut(s) 5074
Bbv12I GWGCWC 5 cut(s) 995, 2882, 4266, 4721, 4902
BceAI ACGGC 1 cut(s) 4650
BcgI CGANNNNNNTGC 4 cut(s) 177, 211, 1853, 1887
BciT130I CCWGG 6 cut(s) 818, 1365, 1770, 5256, 5284, 5604
BciVI GTATCC 2 cut(s) 493, 5639
BclI TGATCA 3 cut(s) 1297, 4396, 4804
BcnI CCSGG 7 cut(s) 857, 1096, 1893, 2333, 2820, 2821, 3548
BcoDI GTCTC 5 cut(s) 2643, 3379, 3646, 4085, 5078
BcuI ACTAGT 1 cut(s) 1304
BfuAI ACCTGC 8 cut(s) 85, 124, 193, 331, 1896, 2366, 2803, 3300
BfuI GTATCC 2 cut(s) 493, 5639
BglII AGATCT 5 cut(s) 3658, 4157, 4240, 4955, 6234
BlpI GCTNAGC 3 cut(s) 3864, 4830, 5832
BmcAI AGTACT 1 cut(s) 393
Bme18I GGWCC 2 cut(s) 1361, 4274
BmeT110I CYCGRG 4 cut(s) 599, 2819, 4231, 5786
BmgBI CACGTC 4 cut(s) 775, 3989, 4360, 5957
BmgT120I GGNCC 3 cut(s) 1361, 4183, 4274
BmiI GGNNCC 7 cut(s) 1362, 2817, 3173, 4185, 4275, 4797, 5102
BoxI GACNNNNGTC 1 cut(s) 4863
BpmI CTGGAG 4 cut(s) 72, 420, 2300, 5117
Bpu10I CCTNAGC 2 cut(s) 1887, 5105
Bpu1102I GCTNAGC 3 cut(s) 3864, 4830, 5832
Bpu14I TTCGAA 4 cut(s) 197, 846, 3759, 3903
BpuEI CTTGAG 5 cut(s) 979, 2581, 3284, 3650, 3926
BpuMI CCSGG 7 cut(s) 857, 1096, 1893, 2333, 2820, 2821, 3548
BsaAI YACGTR 1 cut(s) 5074
BsaBI GATNNNNATC 2 cut(s) 1296, 1617
BsaI GGTCTC 2 cut(s) 3646, 4085
BsaWI WCCGGW 5 cut(s) 908, 1149, 2473, 4582, 4914
BsaXI ACNNNNNCTCC 4 cut(s) 5115, 5145, 5617, 5647
Bse118I RCCGGY 2 cut(s) 2870, 5047
Bse1I ACTGG 5 cut(s) 761, 1428, 2199, 5134, 6015
Bse21I CCTNAGG 1 cut(s) 6195
Bse3DI GCAATG 3 cut(s) 4879, 5138, 6225
Bse8I GATNNNNATC 2 cut(s) 1296, 1617
BseAI TCCGGA 4 cut(s) 908, 1149, 2473, 4582
BseBI CCWGG 6 cut(s) 818, 1365, 1770, 5256, 5284, 5604
BseJI GATNNNNATC 2 cut(s) 1296, 1617
BseMI GCAATG 3 cut(s) 4879, 5138, 6225
BseNI ACTGG 5 cut(s) 761, 1428, 2199, 5134, 6015
BseRI GAGGAG 3 cut(s) 722, 945, 3179
BseSI GKGCMC 2 cut(s) 2820, 4721
Bsh1236I CGCG 1 cut(s) 4572
BshFI GGCC 8 cut(s) 1530, 2568, 3794, 4184, 4302, 5162, 5399, 6051
BshNI GGYRCC 1 cut(s) 2815
BsiHKAI GWGCWC 5 cut(s) 995, 2882, 4266, 4721, 4902
BsiHKCI CYCGRG 4 cut(s) 599, 2819, 4231, 5786
BslFI GGGAC 6 cut(s) 621, 3102, 4287, 4370, 5349, 5619
BsmAI GTCTC 5 cut(s) 2643, 3379, 3646, 4085, 5078
BsmFI GGGAC 6 cut(s) 621, 3102, 4287, 4370, 5349, 5619
BsmI GAATGC 3 cut(s) 578, 734, 2831
BsnI GGCC 8 cut(s) 1530, 2568, 3794, 4184, 4302, 5162, 5399, 6051
Bso31I GGTCTC 2 cut(s) 3646, 4085
BsoBI CYCGRG 4 cut(s) 599, 2819, 4231, 5786
Bsp119I TTCGAA 4 cut(s) 197, 846, 3759, 3903
Bsp1286I GDGCHC 8 cut(s) 461, 995, 2820, 2882, 4266, 4541, 4721, 4902
Bsp13I TCCGGA 4 cut(s) 908, 1149, 2473, 4582
Bsp1720I GCTNAGC 3 cut(s) 3864, 4830, 5832
Bsp19I CCATGG 1 cut(s) 2800
BspANI GGCC 8 cut(s) 1530, 2568, 3794, 4184, 4302, 5162, 5399, 6051
BspEI TCCGGA 4 cut(s) 908, 1149, 2473, 4582
BspFNI CGCG 1 cut(s) 4572
BspHI TCATGA 2 cut(s) 4134, 6105
BspLI GGNNCC 7 cut(s) 1362, 2817, 3173, 4185, 4275, 4797, 5102
BspMAI CTGCAG 3 cut(s) 3126, 3297, 4639
BspMI ACCTGC 8 cut(s) 85, 124, 193, 331, 1896, 2366, 2803, 3300
BspQI GCTCTTC 1 cut(s) 1476
BspT104I TTCGAA 4 cut(s) 197, 846, 3759, 3903
BspT107I GGYRCC 1 cut(s) 2815
BspTNI GGTCTC 2 cut(s) 3646, 4085
BsrDI GCAATG 3 cut(s) 4879, 5138, 6225
BsrFI RCCGGY 2 cut(s) 2870, 5047
BsrI ACTGG 5 cut(s) 761, 1428, 2199, 5134, 6015
BssAI RCCGGY 2 cut(s) 2870, 5047
BssNAI GTATAC 3 cut(s) 1034, 1217, 2999
BssSI CACGAG 3 cut(s) 1971, 3818, 5366
BssT1I CCWWGG 5 cut(s) 2011, 2800, 2841, 3502, 3795
Bst1107I GTATAC 3 cut(s) 1034, 1217, 2999
Bst2BI CACGAG 3 cut(s) 1971, 3818, 5366
Bst2UI CCWGG 6 cut(s) 818, 1365, 1770, 5256, 5284, 5604
Bst6I CTCTTC 3 cut(s) 45, 454, 1476
BstAPI GCANNNNNTGC 2 cut(s) 1843, 3292
BstBAI YACGTR 1 cut(s) 5074
BstBI TTCGAA 4 cut(s) 197, 846, 3759, 3903
BstDSI CCRYGG 2 cut(s) 1952, 2800
BstENI CCTNNNNNAGG 2 cut(s) 4028, 5999
BstFNI CGCG 1 cut(s) 4572
BstHHI GCGC 3 cut(s) 2082, 4616, 4886
BstMAI GTCTC 5 cut(s) 2643, 3379, 3646, 4085, 5078
BstNI CCWGG 6 cut(s) 818, 1365, 1770, 5256, 5284, 5604
BstNSI RCATGY 5 cut(s) 991, 1707, 3206, 3563, 4228
BstPAI GACNNNNGTC 1 cut(s) 4863
BstSLI GKGCMC 2 cut(s) 2820, 4721
BstUI CGCG 1 cut(s) 4572
BstZ17I GTATAC 3 cut(s) 1034, 1217, 2999
Bsu36I CCTNAGG 1 cut(s) 6195
BsuI GTATCC 2 cut(s) 493, 5639
BsuRI GGCC 8 cut(s) 1530, 2568, 3794, 4184, 4302, 5162, 5399, 6051
BtgI CCRYGG 2 cut(s) 1952, 2800
BtrI CACGTC 4 cut(s) 775, 3989, 4360, 5957
BtsI GCAGTG 2 cut(s) 1531, 5680
BveI ACCTGC 8 cut(s) 85, 124, 193, 331, 1896, 2366, 2803, 3300
CaiI CAGNNNCTG 2 cut(s) 3292, 4252
CciI TCATGA 2 cut(s) 4134, 6105
CfoI GCGC 3 cut(s) 2082, 4616, 4886
Cfr10I RCCGGY 2 cut(s) 2870, 5047
Cfr13I GGNCC 3 cut(s) 1361, 4183, 4274
Cfr9I CCCGGG 1 cut(s) 2819
CseI GACGC 1 cut(s) 2642
Csp6I GTAC 8 cut(s) 392, 1569, 2768, 3259, 3486, 3589, 4128, 4325
CspCI CAANNNNNGTGG 2 cut(s) 3328, 3363
CviQI GTAC 8 cut(s) 392, 1569, 2768, 3259, 3486, 3589, 4128, 4325
DraI TTTAAA 4 cut(s) 888, 3493, 3529, 5199
EaeI YGGCCR 2 cut(s) 5160, 5397
Eam1104I CTCTTC 3 cut(s) 45, 454, 1476
EarI CTCTTC 3 cut(s) 45, 454, 1476
Ecl136II GAGCTC 1 cut(s) 2880
Eco130I CCWWGG 5 cut(s) 2011, 2800, 2841, 3502, 3795
Eco24I GRGCYC 3 cut(s) 461, 2882, 4541
Eco31I GGTCTC 2 cut(s) 3646, 4085
Eco32I GATATC 2 cut(s) 1687, 2648
Eco47I GGWCC 2 cut(s) 1361, 4274
Eco53kI GAGCTC 1 cut(s) 2880
Eco72I CACGTG 1 cut(s) 5074
Eco81I CCTNAGG 1 cut(s) 6195
Eco88I CYCGRG 4 cut(s) 599, 2819, 4231, 5786
EcoICRI GAGCTC 1 cut(s) 2880
EcoNI CCTNNNNNAGG 2 cut(s) 4028, 5999
EcoO109I RGGNCCY 1 cut(s) 4183
EcoRI GAATTC 3 cut(s) 1924, 4837, 5906
EcoRII CCWGG 6 cut(s) 816, 1363, 1768, 5254, 5282, 5602
EcoRV GATATC 2 cut(s) 1687, 2648
EcoT14I CCWWGG 5 cut(s) 2011, 2800, 2841, 3502, 3795
EcoT22I ATGCAT 3 cut(s) 580, 1839, 2839
EcoT38I GRGCYC 3 cut(s) 461, 2882, 4541
ErhI CCWWGG 5 cut(s) 2011, 2800, 2841, 3502, 3795
FalI AAGNNNNNCTT 4 cut(s) 3721, 3753, 4913, 4945
FaqI GGGAC 6 cut(s) 621, 3102, 4287, 4370, 5349, 5619
FauI CCCGC 1 cut(s) 530
FauNDI CATATG 2 cut(s) 2002, 3152
FbaI TGATCA 3 cut(s) 1297, 4396, 4804
FblI GTMKAC 7 cut(s) 740, 1033, 1216, 2998, 3625, 3769, 4092
FriOI GRGCYC 3 cut(s) 461, 2882, 4541
FspI TGCGCA 1 cut(s) 2081
GlaI GCGC 3 cut(s) 2081, 4615, 4885
GsuI CTGGAG 4 cut(s) 72, 420, 2300, 5117
HaeIII GGCC 8 cut(s) 1530, 2568, 3794, 4184, 4302, 5162, 5399, 6051
HgaI GACGC 1 cut(s) 2642
HhaI GCGC 3 cut(s) 2082, 4616, 4886
Hin6I GCGC 3 cut(s) 2080, 4614, 4884
HinP1I GCGC 3 cut(s) 2080, 4614, 4884
HincII GTYRAC 7 cut(s) 741, 1310, 1455, 2113, 2367, 3626, 5526
HindII GTYRAC 7 cut(s) 741, 1310, 1455, 2113, 2367, 3626, 5526
HindIII AAGCTT 5 cut(s) 882, 1490, 2617, 2720, 3059
HpaI GTTAAC 1 cut(s) 5526
HphI GGTGA 7 cut(s) 524, 612, 806, 877, 1975, 3905, 5873
Hpy99I CGWCG 5 cut(s) 745, 779, 2225, 5705, 6030
HspAI GCGC 3 cut(s) 2080, 4614, 4884
Kpn2I TCCGGA 4 cut(s) 908, 1149, 2473, 4582
Ksp22I TGATCA 3 cut(s) 1297, 4396, 4804
KspAI GTTAAC 1 cut(s) 5526
LguI GCTCTTC 1 cut(s) 1476
LmnI GCTCC 9 cut(s) 469, 709, 1368, 2700, 2924, 3815, 4585, 4897, 5100
MfeI CAATTG 2 cut(s) 2051, 2852
MhlI GDGCHC 8 cut(s) 461, 995, 2820, 2882, 4266, 4541, 4721, 4902
MlsI TGGCCA 2 cut(s) 5162, 5399
MluI ACGCGT 1 cut(s) 4570
MluNI TGGCCA 2 cut(s) 5162, 5399
MmeI TCCRAC 6 cut(s) 2955, 3837, 4149, 4789, 4990, 5418
Mox20I TGGCCA 2 cut(s) 5162, 5399
Mph1103I ATGCAT 3 cut(s) 580, 1839, 2839
MroI TCCGGA 4 cut(s) 908, 1149, 2473, 4582
MroXI GAANNNNTTC 4 cut(s) 945, 1470, 1492, 3909
MscI TGGCCA 2 cut(s) 5162, 5399
MslI CAYNNNNRTG 4 cut(s) 4347, 4790, 5311, 5376
Msp20I TGGCCA 2 cut(s) 5162, 5399
MspA1I CMGCKG 5 cut(s) 161, 230, 299, 1198, 4829
MunI CAATTG 2 cut(s) 2051, 2852
Mva1269I GAATGC 3 cut(s) 578, 734, 2831
MvaI CCWGG 6 cut(s) 818, 1365, 1770, 5256, 5284, 5604
MvnI CGCG 1 cut(s) 4572
NciI CCSGG 7 cut(s) 857, 1096, 1893, 2333, 2820, 2821, 3548
NcoI CCATGG 1 cut(s) 2800
NdeI CATATG 2 cut(s) 2002, 3152
NlaIV GGNNCC 7 cut(s) 1362, 2817, 3173, 4185, 4275, 4797, 5102
NmeAIII GCCGAG 1 cut(s) 4079
NmuCI GTSAC 8 cut(s) 1757, 1981, 3176, 3335, 3443, 4019, 4601, 5990
NsbI TGCGCA 1 cut(s) 2081
NsiI ATGCAT 3 cut(s) 580, 1839, 2839
NspI RCATGY 5 cut(s) 991, 1707, 3206, 3563, 4228
NspV TTCGAA 4 cut(s) 197, 846, 3759, 3903
PacI TTAATTAA 1 cut(s) 1809
PaeI GCATGC 1 cut(s) 3206
PaeR7I CTCGAG 2 cut(s) 599, 5786
PagI TCATGA 2 cut(s) 4134, 6105
PaqCI CACCTGC 4 cut(s) 85, 124, 2803, 3300
PciI ACATGT 2 cut(s) 987, 3559
PciSI GCTCTTC 1 cut(s) 1476
PctI GAATGC 3 cut(s) 578, 734, 2831
PdmI GAANNNNTTC 4 cut(s) 945, 1470, 1492, 3909
PflFI GACNNNGTC 1 cut(s) 2274
PflMI CCANNNNNTGG 1 cut(s) 2320
PfoI TCCNGGA 2 cut(s) 5254, 5282
PmaCI CACGTG 1 cut(s) 5074
PmlI CACGTG 1 cut(s) 5074
Ppu21I YACGTR 1 cut(s) 5074
PscI ACATGT 2 cut(s) 987, 3559
PshAI GACNNNNGTC 1 cut(s) 4863
PshBI ATTAAT 3 cut(s) 573, 1809, 5609
PsiI TTATAA 2 cut(s) 1382, 5592
Psp124BI GAGCTC 1 cut(s) 2882
Psp1406I AACGTT 1 cut(s) 5415
Psp6I CCWGG 6 cut(s) 816, 1363, 1768, 5254, 5282, 5602
PspCI CACGTG 1 cut(s) 5074
PspGI CCWGG 6 cut(s) 816, 1363, 1768, 5254, 5282, 5602
PspN4I GGNNCC 7 cut(s) 1362, 2817, 3173, 4185, 4275, 4797, 5102
PspPI GGNCC 3 cut(s) 1361, 4183, 4274
PstI CTGCAG 3 cut(s) 3126, 3297, 4639
PstNI CAGNNNCTG 2 cut(s) 3292, 4252
PsyI GACNNNGTC 1 cut(s) 2274
PvuII CAGCTG 1 cut(s) 1198
RsaI GTAC 8 cut(s) 393, 1570, 2769, 3260, 3487, 3590, 4129, 4326
RsaNI GTAC 8 cut(s) 392, 1569, 2768, 3259, 3486, 3589, 4128, 4325
RseI CAYNNNNRTG 4 cut(s) 4347, 4790, 5311, 5376
SacI GAGCTC 1 cut(s) 2882
SalI GTCGAC 2 cut(s) 739, 3624
SapI GCTCTTC 1 cut(s) 1476
Sau96I GGNCC 3 cut(s) 1361, 4183, 4274
ScaI AGTACT 1 cut(s) 393
SduI GDGCHC 8 cut(s) 461, 995, 2820, 2882, 4266, 4541, 4721, 4902
Sfr274I CTCGAG 2 cut(s) 599, 5786
SfuI TTCGAA 4 cut(s) 197, 846, 3759, 3903
SinI GGWCC 2 cut(s) 1361, 4274
SlaI CTCGAG 2 cut(s) 599, 5786
SmaI CCCGGG 1 cut(s) 2821
SmiMI CAYNNNNRTG 4 cut(s) 4347, 4790, 5311, 5376
SmlI CTYRAG 7 cut(s) 599, 994, 2560, 3263, 3629, 3941, 5786
SmoI CTYRAG 7 cut(s) 599, 994, 2560, 3263, 3629, 3941, 5786
SpeI ACTAGT 1 cut(s) 1304
SphI GCATGC 1 cut(s) 3206
SspI AATATT 2 cut(s) 1520, 4941
SstI GAGCTC 1 cut(s) 2882
StyI CCWWGG 5 cut(s) 2011, 2800, 2841, 3502, 3795
TatI WGTACW 3 cut(s) 391, 2767, 3485
TauI GCSGC 6 cut(s) 233, 565, 1107, 1950, 2247, 3583
TseFI GTSAC 8 cut(s) 1757, 1981, 3176, 3335, 3443, 4019, 4601, 5990
Tsp45I GTSAC 8 cut(s) 1757, 1981, 3176, 3335, 3443, 4019, 4601, 5990
TspGWI ACGGA 9 cut(s) 3164, 3767, 4073, 4691, 4702, 5193, 5621, 5711, 6044
TspMI CCCGGG 1 cut(s) 2819
Tth111I GACNNNGTC 1 cut(s) 2274
Van91I CCANNNNNTGG 1 cut(s) 2320
VneI GTGCAC 1 cut(s) 4717
VpaK11BI GGWCC 2 cut(s) 1361, 4274
VspI ATTAAT 3 cut(s) 573, 1809, 5609
XagI CCTNNNNNAGG 2 cut(s) 4028, 5999
XbaI TCTAGA 7 cut(s) 1285, 2182, 4000, 4381, 5968, 6178, 6229
XceI RCATGY 5 cut(s) 991, 1707, 3206, 3563, 4228
XcmI CCANNNNNNNNNTGG 3 cut(s) 4772, 4973, 5262
XhoI CTCGAG 2 cut(s) 599, 5786
XmaI CCCGGG 1 cut(s) 2819
XmiI GTMKAC 7 cut(s) 740, 1033, 1216, 2998, 3625, 3769, 4092
XmnI GAANNNNTTC 4 cut(s) 945, 1470, 1492, 3909
ZrmI AGTACT 1 cut(s) 393
Zsp2I ATGCAT 3 cut(s) 580, 1839, 2839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.