Rroxscaffold_2G00092830

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000307
Physical Location & Seq
Forward (+)
14302699 .. 14305929
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092830.1

Sequence Viewer

Length: 1404 bp
ATGGAATTGACACATTTAGGGATGCTGAAAAACTCCAGAAGGGAAATTCCGTGGTACCAACGCTCGTTGAAGCCATCCGAAGATCCAGATGTGCTGTCATTGTTTTTTTCAGCAAACTATGCTTCTTCAGCATGGTGTTTGAATGAACTTGTGCATATTCTTGAGTGCAAGAAAGCGGGAGGAAGCAAACTGGAGGTGTTTCCGGTTTTCTATAACGTCGAACCGACCGAGGTCAGAAAGCAAACTGGAGTTTACGGGGAAGCTTTGTCTGCGCATGAAGGTCTGAACAAGGTGGACATATGGAAGAATGCCTCGACTGAAGTAGCGAATTTCTCCGAATGGGATGTGAAAAATAGAGATGCAGATATAGATACAGATGACATGGGAAAATATTTGTTATGGAAAGGACTATGTTCAAAGAAGGTGCTCATCATTCTAGACGATGTTCATGAACTCAAACAAATGGAAGCTCTTGCTGGAAGTAGTAAACAGAATCCTTGGTTTGGTCCAGGGAGTAGAGTAATTATAACAACTAGAGATGAGCAGTTGTTGAAGTCTTACGACGTGCACAAGATATACGAGGTTGAGAAACTGACTGATGCTGAAGCTTCTCAGCTCTTGTGTCAGAAAGCCTTCAGGAAAAACCATGCTCCAACGGAATATCTAAAGCTGTCCAACAATTTTGTAAAATATGCCAGTGGCCTTCCTTTAGCTCTTGAAGTTCTGGGTTCATACTTGTGGGAGAAGGAGGTAAGTGAATGGTCAGCAGCATTGGTGAGACTATATGACGATCCTGAAAATAAAATTTTGAGTGTGCTTCAATTAAGTTATGATGGATTGAAGTCAACAGTGAAGGACATGTTTCTGGACATTGCATGTTTTTTCCGAGGAGAGGACCAAGTTCGTGCAATGAAGATCTTCATGAGTTGTGACTTTCATCCTGAAATTGGCATTGTTGACCTTATTGATAAATCTTTGATTAGAATTGAAGGAAAAAAACTGTGGATGCATGATTTACTACAACAAATGGATTGGCAGATTGTTCGTCAAGAATTTCCTAAAGAGCCGGGTAAACGTAGTAGGTTGTGGCTTGATGAAAATGCTTGTGAGTACGAACTCTGGAGGTCGTGGTTTGATGACGAACGGTTGAGCAAACACAGAAGGTGGTGGCTTGACAAGGATGCTCGTGCTGTACTCATACATAATCTGTTACTTGAACTTGAAAGAATAGCTTTAGTAAATCTTAACAAATTCTCTTTGGAAGTGAAAGAGTGTGGAATACAACTAATATCCCAGCAAGGTGCTGCATTGTCTGTTCAAGATTTAACACAGACAGCTAGTGATGAACTTCATCAGCTGGATTCAAATTTTGTTCCTCCACATATCGTAACGGAGGAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

467

Amino Acids

54.02

Weight (kDa)

5.34

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 36 - 119 1.1e-20 TIR domain
NB-ARC PF00931 119 - 215 7.1e-10 NB-ARC domain
WHD_ROQ1 PF23282 285 - 350 3.8e-15 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
Acc16I TGCGCA 1 cut(s) 273
Acc65I GGTACC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 54
AciI CCGC 1 cut(s) 176
AclWI GGATC 2 cut(s) 77, 785
AcsI RAATTY 6 cut(s) 45, 328, 804, 1052, 1250, 1366
AcuI CTGAAG 4 cut(s) 111, 339, 619, 624
AfaI GTAC 3 cut(s) 56, 1112, 1194
AfiI CCNNNNNNNGG 3 cut(s) 503, 892, 947
AflIII ACRYGT 1 cut(s) 858
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 508
AluBI AGCT 9 cut(s) 263, 470, 608, 616, 670, 713, 1232, 1337, 1357
AluI AGCT 9 cut(s) 263, 470, 608, 616, 670, 713, 1232, 1337, 1357
Alw21I GWGCWC 2 cut(s) 429, 570
Alw26I GTCTC 1 cut(s) 772
Alw44I GTGCAC 1 cut(s) 566
AlwI GGATC 2 cut(s) 77, 785
AoxI GGCC 1 cut(s) 700
ApaLI GTGCAC 1 cut(s) 566
ApeKI GCWGC 2 cut(s) 767, 1304
ApoI RAATTY 6 cut(s) 45, 328, 804, 1052, 1250, 1366
Asp700I GAANNNNTTC 2 cut(s) 632, 917
Asp718I GGTACC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 2 cut(s) 506, 895
AsuC2I CCSGG 1 cut(s) 1068
AsuHPI GGTGA 1 cut(s) 787
AvaII GGWCC 2 cut(s) 506, 895
BaeGI GKGCMC 1 cut(s) 570
BanI GGYRCC 1 cut(s) 54
BauI CACGAG 1 cut(s) 1185
Bbv12I GWGCWC 2 cut(s) 429, 570
BbvI GCAGC 2 cut(s) 779, 1291
BccI CCATC 2 cut(s) 82, 827
BcgI CGANNNNNNTGC 2 cut(s) 1025, 1059
BciT130I CCWGG 1 cut(s) 510
BcnI CCSGG 1 cut(s) 1068
BcoDI GTCTC 1 cut(s) 772
BfaI CTAG 3 cut(s) 437, 534, 1338
BglII AGATCT 1 cut(s) 915
BisI GCNGC 2 cut(s) 768, 1305
BlsI GCNGC 2 cut(s) 769, 1306
Bme1390I CCNGG 2 cut(s) 510, 1068
Bme18I GGWCC 2 cut(s) 506, 895
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 506, 895
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 2 cut(s) 510, 1068
BmsI GCATC 5 cut(s) 12, 349, 589, 996, 1171
BoxI GACNNNNGTC 1 cut(s) 230
BpmI CTGGAG 4 cut(s) 19, 212, 267, 1141
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 1068
BsaJI CCNNGG 5 cut(s) 50, 228, 497, 509, 886
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 892, 947
Bse1I ACTGG 3 cut(s) 195, 250, 696
Bse3DI GCAATG 2 cut(s) 870, 915
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 5 cut(s) 50, 228, 497, 509, 886
BseGI GGATG 6 cut(s) 27, 74, 349, 937, 1011, 1186
BseLI CCNNNNNNNGG 3 cut(s) 503, 892, 947
BseMI GCAATG 2 cut(s) 870, 915
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 3 cut(s) 195, 250, 696
BseRI GAGGAG 1 cut(s) 903
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 2 cut(s) 779, 1291
BseYI CCCAGC 1 cut(s) 1293
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 429, 570
BsiSI CCGG 2 cut(s) 203, 1067
BslI CCNNNNNNNGG 3 cut(s) 503, 892, 947
BsmAI GTCTC 1 cut(s) 772
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 429, 570
Bsp143I GATC 3 cut(s) 82, 790, 915
BspACI CCGC 1 cut(s) 176
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 1 cut(s) 625
BspHI TCATGA 2 cut(s) 448, 921
BspLI GGNNCC 1 cut(s) 56
BspPI GGATC 2 cut(s) 77, 785
BspT107I GGYRCC 1 cut(s) 54
BsrDI GCAATG 2 cut(s) 870, 915
BsrI ACTGG 3 cut(s) 195, 250, 696
BssECI CCNNGG 5 cut(s) 50, 228, 497, 509, 886
BssMI GATC 3 cut(s) 82, 790, 915
BssSI CACGAG 1 cut(s) 1185
BssT1I CCWWGG 1 cut(s) 497
Bst2BI CACGAG 1 cut(s) 1185
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 3 cut(s) 850, 1002, 1146
BstAPI GCANNNNNTGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 612
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 6 cut(s) 27, 74, 349, 937, 1011, 1186
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 3 cut(s) 85, 793, 918
BstMAI GTCTC 1 cut(s) 772
BstMBI GATC 3 cut(s) 82, 790, 915
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 3 cut(s) 119, 128, 269
BstNI CCWGG 1 cut(s) 510
BstNSI RCATGY 2 cut(s) 862, 879
BstPAI GACNNNNGTC 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 508, 1066
BstSLI GKGCMC 1 cut(s) 570
BstV1I GCAGC 2 cut(s) 779, 1291
BstX2I RGATCY 2 cut(s) 82, 915
BstYI RGATCY 2 cut(s) 82, 915
BsuRI GGCC 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 50
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 6 cut(s) 27, 74, 349, 937, 1011, 1186
BtsIMutI CAGTG 2 cut(s) 703, 855
CciI TCATGA 2 cut(s) 448, 921
CfoI GCGC 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 506, 895
Csp6I GTAC 3 cut(s) 55, 1111, 1193
CviAII CATG 9 cut(s) 132, 275, 382, 449, 647, 859, 876, 922, 1010
CviQI GTAC 3 cut(s) 55, 1111, 1193
DdeI CTNAG 1 cut(s) 612
DpnI GATC 3 cut(s) 84, 792, 917
DpnII GATC 3 cut(s) 82, 790, 915
Eco130I CCWWGG 1 cut(s) 497
Eco47I GGWCC 2 cut(s) 506, 895
Eco57I CTGAAG 4 cut(s) 111, 339, 619, 624
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 497
EcoT22I ATGCAT 1 cut(s) 1011
ErhI CCWWGG 1 cut(s) 497
FaeI CATG 9 cut(s) 135, 278, 385, 452, 650, 862, 879, 925, 1013
FalI AAGNNNNNCTT 2 cut(s) 1216, 1248
FatI CATG 9 cut(s) 131, 274, 381, 448, 646, 858, 875, 921, 1009
FauI CCCGC 1 cut(s) 169
FauNDI CATATG 1 cut(s) 299
Fnu4HI GCNGC 2 cut(s) 768, 1305
FokI GGATG 6 cut(s) 34, 61, 356, 924, 1018, 1193
Fsp4HI GCNGC 2 cut(s) 768, 1305
FspBI CTAG 3 cut(s) 437, 534, 1338
FspI TGCGCA 1 cut(s) 273
GlaI GCGC 1 cut(s) 273
GluI GCNGC 2 cut(s) 768, 1305
GsaI CCCAGC 1 cut(s) 1297
GsuI CTGGAG 4 cut(s) 19, 212, 267, 1141
HaeIII GGCC 1 cut(s) 702
HapII CCGG 2 cut(s) 203, 1067
HhaI GCGC 1 cut(s) 274
Hin1II CATG 9 cut(s) 135, 278, 385, 452, 650, 862, 879, 925, 1013
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HincII GTYRAC 2 cut(s) 846, 958
HindII GTYRAC 2 cut(s) 846, 958
HindIII AAGCTT 2 cut(s) 261, 606
HinfI GANTC 2 cut(s) 493, 1361
HpaII CCGG 2 cut(s) 203, 1067
HphI GGTGA 1 cut(s) 787
Hpy166II GTNNAC 7 cut(s) 253, 295, 488, 568, 846, 958, 1073
Hpy188I TCNGA 6 cut(s) 79, 236, 285, 337, 627, 887
Hpy8I GTNNAC 7 cut(s) 253, 295, 488, 568, 846, 958, 1073
Hpy99I CGWCG 2 cut(s) 221, 566
HpyAV CCTTC 9 cut(s) 33, 272, 415, 643, 713, 739, 847, 983, 1155
HpyCH4III ACNGT 3 cut(s) 850, 1002, 1146
HpyCH4IV ACGT 3 cut(s) 216, 564, 1075
HpyCH4V TGCA 8 cut(s) 154, 168, 362, 568, 875, 908, 1009, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 128, 269
HpyF3I CTNAG 1 cut(s) 612
HpySE526I ACGT 3 cut(s) 216, 564, 1075
Hsp92II CATG 9 cut(s) 135, 278, 385, 452, 650, 862, 879, 925, 1013
HspAI GCGC 1 cut(s) 272
KpnI GGTACC 1 cut(s) 58
Kzo9I GATC 3 cut(s) 82, 790, 915
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 2 cut(s) 779, 1291
LweI GCATC 5 cut(s) 12, 349, 589, 996, 1171
MaeI CTAG 3 cut(s) 437, 534, 1338
MaeII ACGT 3 cut(s) 216, 564, 1075
MaeIII GTNAC 3 cut(s) 929, 1209, 1387
MalI GATC 3 cut(s) 84, 792, 917
MboI GATC 3 cut(s) 82, 790, 915
MboII GAAGA 5 cut(s) 92, 117, 316, 910, 925
MflI RGATCY 2 cut(s) 82, 915
MhlI GDGCHC 2 cut(s) 429, 570
MmeI TCCRAC 2 cut(s) 677, 699
Mph1103I ATGCAT 1 cut(s) 1011
MroXI GAANNNNTTC 2 cut(s) 632, 917
MseI TTAA 3 cut(s) 824, 1245, 1325
MslI CAYNNNNRTG 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 1357
MspI CCGG 2 cut(s) 203, 1067
MspR9I CCNGG 2 cut(s) 510, 1068
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 1 cut(s) 510
MwoI GCNNNNNNNGC 3 cut(s) 119, 128, 269
NciI CCSGG 1 cut(s) 1068
NdeI CATATG 1 cut(s) 299
NdeII GATC 3 cut(s) 82, 790, 915
NlaIII CATG 9 cut(s) 135, 278, 385, 452, 650, 862, 879, 925, 1013
NlaIV GGNNCC 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 929
NsbI TGCGCA 1 cut(s) 273
NsiI ATGCAT 1 cut(s) 1011
NspI RCATGY 2 cut(s) 862, 879
PagI TCATGA 2 cut(s) 448, 921
PciI ACATGT 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 313
PdmI GAANNNNTTC 2 cut(s) 632, 917
PfeI GAWTC 2 cut(s) 493, 1361
PkrI GCNGC 2 cut(s) 769, 1306
PscI ACATGT 1 cut(s) 858
PshAI GACNNNNGTC 1 cut(s) 230
PsiI TTATAA 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 508
PspFI CCCAGC 1 cut(s) 1293
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 2 cut(s) 506, 895
PsuI RGATCY 2 cut(s) 82, 915
PvuII CAGCTG 1 cut(s) 1357
RsaI GTAC 3 cut(s) 56, 1112, 1194
RsaNI GTAC 3 cut(s) 55, 1111, 1193
RseI CAYNNNNRTG 1 cut(s) 736
SaqAI TTAA 3 cut(s) 824, 1245, 1325
SatI GCNGC 2 cut(s) 768, 1305
Sau3AI GATC 3 cut(s) 82, 790, 915
Sau96I GGNCC 2 cut(s) 506, 895
ScrFI CCNGG 2 cut(s) 510, 1068
SduI GDGCHC 2 cut(s) 429, 570
SfaNI GCATC 5 cut(s) 12, 349, 589, 996, 1171
SinI GGWCC 2 cut(s) 506, 895
SmiMI CAYNNNNRTG 1 cut(s) 736
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SsiI CCGC 1 cut(s) 176
SspI AATATT 1 cut(s) 392
SspMI CTAG 3 cut(s) 437, 534, 1338
StyD4I CCNGG 2 cut(s) 508, 1066
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 3 cut(s) 850, 1002, 1146
TaiI ACGT 3 cut(s) 219, 567, 1078
TaqI TCGA 2 cut(s) 219, 314
TaqII GACCGA 1 cut(s) 242
TatI WGTACW 1 cut(s) 1192
TfiI GAWTC 2 cut(s) 493, 1361
Tru1I TTAA 3 cut(s) 824, 1245, 1325
Tru9I TTAA 3 cut(s) 824, 1245, 1325
TscAI CASTG 2 cut(s) 703, 855
TseFI GTSAC 1 cut(s) 929
TseI GCWGC 2 cut(s) 767, 1304
Tsp45I GTSAC 1 cut(s) 929
TspGWI ACGGA 2 cut(s) 39, 671
TspRI CASTG 2 cut(s) 703, 855
VneI GTGCAC 1 cut(s) 566
VpaK11BI GGWCC 2 cut(s) 506, 895
XapI RAATTY 6 cut(s) 45, 328, 804, 1052, 1250, 1366
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 2 cut(s) 862, 879
XmnI GAANNNNTTC 2 cut(s) 632, 917
XspI CTAG 3 cut(s) 437, 534, 1338
Zsp2I ATGCAT 1 cut(s) 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.