Rorug02G0455600

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000307
Physical Location & Seq
Forward (+)
58538425 .. 58547244
8820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0455600.1

Sequence Viewer

Length: 5247 bp
ATGAATGCACAGGCGCATATGTCGGGACAAATCTCTGGGCAGGTGCCCAATCAGACAGGTTCTCAGTTGCCTGTACTGCCCCAACATAACGGAAATGCCATTCCTCCCCAAATGCAAAATGTAGGCGGGCCACCTCGTGCGATCAACGCTATGGACCCTGAGCTTCTTAGAGCTCGTCACTTTATGCAGGAGAAGATCTGTCGTGTTATACAGCAGCGGCAACTTCCCCAGCCAATGAATGAAAAGAAGTTTAGGGATATTGTTAAACGCTTGGAGGAGGGATTGTTGAGATCAGCTGTCAACAAGGAGGACTATATGAACCTGGACACATTAGAGAGCCGTTTGCATAATCTGATCAAGCGTCCTCCTCAGACCAACCAGAGCCAACAGTATCCACAGCTTGTTAATTCTTCCTCTCCTGTTGGTACGATGATACCGACCCCTGGCATGTCACACAGTGGGAACTCAAATATGATGGTTACTTCTTCCATGGACGCTTCTATGATCAATACTGGTGGAACTACTAGCATACCACCCACAACGGTCAGCACAGGAAACATGTTGCCAGGTGGTGGCTTACATGGTTCTATGTCTAATGGATATCAGCAGTCTCCTGCCAATTTCTCCATTGGTTCTGGAGGAAACATGTCGTCGATGGGTGCACAAAGAATTGCAAGCCAAATGATTCCCACTCCTGGGTTTAATAACAGTGGTAACCAATCTTACATGAATTTGGAATCCTCTAATAACAGTGGTGGGTTCTCTACTGTTGATTCTTCAATGGTAACGCAACCGCAACAGCAAAAGCAGCATATTGGTAGTCAAAACAGCCGTATGCTTCATAACCTTGGCAGCCAAGGGAGTAGTGGAATGAGGTCCGGCTTGCAGCAGAAATCGTATGGGGTGTCAAATGGGTCTATAAATGGTGGAATGGGAATGATTGCAAACAATTTACCAATTGTTAATGAAACTGGCATTTCGGATAGCTATCTGACCCCCACAGCTTATGCCAATTCTTCCAAACCCTTACAGCAACACTTTGATCCACATCAGCGGCCTGTAATGCAAGGTGATGGCTACGGCATAAACAATGCTGATTCTTTTGGGTCTGGAAATTACTATGGTGCACCGGCTTCTGGGTCAATGATGAATCCTCAAAATTTGAATTCAGTCAGCATGACACCTATATCCAAGACCAGCTCTCCCCTTATAAGTAATCAGTCCAATATGCATACTCAACAACAAAGTCTCCATGTGAAGCCTCAACAATTTGATCAATTGGAAAAGATGAGTTTCCAAAATTCTTCAAGAGACAATATGCTCCAATCTCATCAGCACCAGCAATTTCAGCAGCAACCCAATCAATTTCAACAGCAGCAGCAATTAGTTCACAACCAACGCCAACAGAAGCAGCAAAATCAGCAAGCCCAGCATTTGTCAAGTAATGATGTTTTTGTTCAGTCTCCGATGACATCTGATTTAAGCAGTCAAGCAAAGCGTGATAATGAAGTTATGCACTCACAAACAGAACAGTTCCAAATGTCGGAGATGCATAATCAATACCATCAGCACTCTGCTGAAGATCGTTTGAGGAATACTCAGCATAACACATCTGGTCAGCATGATCTCTCCTCTTCATCGTCCCAAACTTCTCAGCAAATGCAACAAATGTTGCATCCTCACCAATTGATTGCAGAGACTCGAAATGATTTCAGTTCTCTTTCTGTTGGAGCTCAATCAGAATCAGCACTTCAGGGTCAATGGCGTCCTCAATTGCAAGATGGGAGTCACAGGCAGGTCCACATGTCACATGATCAGCATGTCCAAGAAGATTTCCGTCAGAGAATATCTGGGCAAGATGAAGCTCAGTGTAATAATCTTTCTTCAGAAGGACCTAATGTTAGTCAAACTGTTGCCTCTAGAAGCACATCTCATCCCCAGAATCAGGATCGGTTCAGAAATCAACAAAGATGGCTTTTGTTCTTGCGCCATGCTCGTAAATGTCCATCTTCTGAAGGGAAATGTAAAGAATCTCATTGTTTAACAGCGCAAAAGTTGTTGAAACATATTGGAAAATGCATGTCATTCCAATGCACATATCCTTACTGTCCTCGTACCAAGAAGTTGATTCATCATCACAAGAGTTGCTCGGATTCAGCTTGCCCAGTTTGTGTTCCTGTCAAGAATTATATTCAGACACATAGTAAGGTGCAGACCCGTCTTGTTCCTGAATCTGGTGTTCAGAATGGATCTTGTAAAGCCTATGATAGTGTTGACACTTCTGCTACATTGATTTCTAAGATTCCTCCAGTTGCTGAAACTTCTGAAGAGCCGCAACCATCTATGAAACGCTTGAAGATAGAGCAGTCCTCTCAATCTATTGTTCCTGATTCTGTAAGTAATGCAGCAACAGCTTCTGCAATTAAAGAACCCCATGTATCCCAGGATACACAGATTCAGGATTACCAACAGAGTGAAATTTCTATGCCAATTAAATCTGAGTTTAAGGAAGTGAAAATAGAAGCTTCTTTAAGTTCTGGACATGGGAATCTTGATGAGATGAAGGATAGTTTTGAGGAGAACTGCAATCAAAGGCAGGATAGTGTGCCTGCCCCTTATAATGAGCCTGCTGGTTTAGCTAAGCAAGAAAGCATCAAACTTGAGAAAGAAATTGATCCAGCTAAACAAGAAAATGCTATGCAGACCGTGGAAAATCCAGCTGGAACCAAGTCTGGGAAGCCTAAAATAAAGGGCGTGTCGTTGACTGAATTGTTCACTCCAGAGCAAGTTAGGGCACACATTACAGGTCTCAGACAGTGGGTTGGCCAGAGCAAAGCAAAGGCAGAGAAGAACCAAGCAATGGAACATGCAATGAGTGAGAACTCCTGTCAGTTGTGTGCAGTAGAGAAGCTTACGTTTGAACCACCGCCTATGTATTGTACACCTTGTGGTGCTCGTATAAAGCGCAATTCTATGTATTATACAATGGGGGCTGGTGACACACGGCATTATTTTTGTATTCCATGCTATAATGAGGCTCGTGGAGATACAATAATTGTTGATGGGACTGCCATTCCAAAGGCAAGGCTAGAGAAGAAGAAAAATGATGAGGAAACAGAAGAATGGTGGGTCCAATGTGACAAGTGTGAAGCTTGGCAACATCAAATCTGTGCTTTGTTCAATGGCCGAAGAAATGATGGTGGACAAGCTGAGTACACTTGCCCTAATTGCTATATACAAGAGGTTGAAAGGGGAGAGCGTAAGCCATTACCGCAGAGTGCTGTTTTGGGGGCCAAAGATTTGCCAAGGACAATACTCAGCGACCACATAGAGCAACGGTTATTTAAGAAACTAAAGGTGGAAAGACTAGAGAGGGCAAGACAGCAGGGGAAAAGTTATGATGAGGTTCCTGGAGCAGAATCACTTGTAATTAGAGTTGTGTCCTCTGTCGACAAAAAATTGGAAGTAAAGCAGCGGTTTTTAGAAATATTTCAGGAAGACAATTATCCCACGGAGTTCCCATACAAATCTAAGGTTGTATTGCTGTTCCAGAAAATTGAAGGTGTAGAAGTATGCTTATTCGGCATGTATGTTCAAGAATTTGGAGCCGAATGTCAATTTCCAAATCAGCGCCGTGTTTATCTATCGTATTTGGATTCGGTTAAGTATTTCAGGCCCGAGGTTAAGGCGGTGACTGGAGAAGCTCTCCGTACATATGTTTACCATGAAATTCTAATTGGATACCTCGAATATTGCAAGCTACGAGGGTTTACAAGCTGCTACATTTGGGCATGTCCTCCATTGAAGGGTGAAGATTATATTTTATATTGTCATCCGGAAATTCAGAAAACACCGAAATCTGATAAGCTTCGTGAATGGTATTTGGCAATGCTTAGGAAAGCCTCTAAGGAAAACATCGTAGTCGAGCTCACTAATTTATACGATCATTTCTTTGTAACTAATGGTGAAGGCAAGGCTAAGGTCACTGCAGCTAGGCTGCCCTACTTTGATGGTGACTACTGGCCCGGTGCTGCCGAGGATCTCATTTATCAAATGCGTCAAGATGAGGATGGGAGAAAACAGAATAAGAAAGGATCAACCAAAAAGACTATAACAAAAAGGGCTCTAAAAGCATCTGGGCAGACTGATCTCTCTGGTAACGCGTCAAAGGACCTGCTCCTAATGCACAAACTTGGTGAAACTATTAGCCCGATGAAGGAAGATTTCATCATGGTTCATTTGCAGCACGCTTGCTCTCACTGCTGTGTACTCATGGTATCTGGAAAACGTTGGGCTTGCAATCAATGCAGATATTTTCAGCTATGTGATAAGTGTTATGAAGCAGAGCAGAAACGGGAAGAAAGGGAGAGACACCCTATGAATATGAGGGATAAACATGACTTCCGTCCTTTTGACATCACTGATGTACCAGTGGATACGAAGGACAGAGATGAGATTCTTGAGAGTGAATTCTTTGACACTAGACAGGCGTTTCTGAGTCTTTGTCAAGGGAATCATTATCAGTATGATACTTTACGGAGGGCTAAACATTCCTCAATGATGGTCCTTTACCATCTTCATAATCCAACGGCTCCTGCATTTGTGACAACATGCAATATATGTCATCTCGACATTGAAGCAGGTCAAGGTTGGCGTTGTGAAGTTTGCCCTGAATATGATGTGTGCAATAGTTGTTATCAGAAGGATGGTGGTGTCGATCATCATCATAAATTGACAAATCATCCATCCATTGCTGACCGTGATGCACAAAACAAGGAAGCTAGGCAAATGCGAGTTGTACAGCTTAGGAGAATGCTTGATCTTCTAGTGCACGCATCACAATGTCGTTCTGCACAATGTATGTACCCCAATTGTCGCAAGGTCAAGGGACTTTTTCGCCATGGAATTCAATGTAAAGTTCGTGCATCTGGAGGTTGTGTTCTTTGTAAGAAAATGTGGTATCTCCTGCAACTTCATGCTCGAGCTTGCAAAGTATCCGAGTGCCATGTGCCTCGTTGCAGAGATCTAAAAGAACATTTGAGGAGGCTGCAGCAACAGTCTGATTCAAGGCGAAGAGCTGCCGTGATGGAGATGATGAGACAGAGAGCTGCCGAGATAAACAATTCTGGGGAACAATTCTTTGGTTCTTATCTCCTTGACTCAATCGTCCCCAAGAGAAGACATGCAAAAATGATCAACTCGAGCTTGCCCTCTTCAACAGCTGCCAGCAATGCCCCGTTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1748

Amino Acids

197.36

Weight (kDa)

8.6

Isoelectric Point (pI)

57.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-TAZ PF02135 655 - 724 5.7e-16 TAZ zinc finger
PHD PF00628 1037 - 1077 9e-06 PHD-finger
HAT_KAT11 PF08214 1135 - 1351 2.6e-38 Histone acetylation protein
ZZ PF00569 1535 - 1575 8.1e-07 Zinc finger, ZZ type
zf-TAZ PF02135 1603 - 1673 1.9e-13 TAZ zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1211, 2619
AarI CACCTGC 1 cut(s) 31
AasI GACNNNNNNGTC 1 cut(s) 5165
Acc36I ACCTGC 4 cut(s) 31, 1786, 4182, 4631
AccB1I GGYRCC 1 cut(s) 43
AccB7I CCANNNNNTGG 2 cut(s) 572, 2860
AccI GTMKAC 1 cut(s) 3450
AccII CGCG 1 cut(s) 4163
AccIII TCCGGA 1 cut(s) 3835
AciI CCGC 9 cut(s) 126, 217, 794, 1054, 2333, 2927, 3272, 3475, 3689
AclI AACGTT 1 cut(s) 4288
AclWI GGATC 6 cut(s) 1037, 1956, 2257, 2669, 4047, 4102
AcoI YGGCCR 2 cut(s) 2824, 3184
AcuI CTGAAG 5 cut(s) 1599, 1736, 1869, 2034, 2346
AcyI GRCGYC 1 cut(s) 1765
AdeI CACNNNGTG 3 cut(s) 137, 458, 2948
AflIII ACRYGT 4 cut(s) 558, 645, 1803, 4161
AjnI CCWGG 6 cut(s) 321, 442, 565, 694, 2442, 3409
AjuI GAANNNNNNNTTGG 4 cut(s) 3720, 3752, 5124, 5156
AleI CACNNNNGTG 1 cut(s) 4263
AloI GAACNNNNNNTCC 2 cut(s) 4923, 4955
Alw21I GWGCWC 7 cut(s) 175, 664, 1129, 1735, 2956, 3930, 4833
Alw26I GTCTC 8 cut(s) 615, 1253, 1305, 1467, 1691, 2813, 4363, 5092
Alw44I GTGCAC 3 cut(s) 660, 1125, 4829
AlwI GGATC 6 cut(s) 1037, 1956, 2257, 2669, 4047, 4102
AlwNI CAGNNNCTG 2 cut(s) 2315, 2417
Ama87I CYCGRG 3 cut(s) 3677, 4980, 5200
Aor13HI TCCGGA 1 cut(s) 3835
AoxI GGCC 7 cut(s) 128, 1055, 2824, 3184, 3291, 3674, 4022
ApaLI GTGCAC 3 cut(s) 660, 1125, 4829
Asp700I GAANNNNTTC 1 cut(s) 3489
AspLEI GCGC 5 cut(s) 16, 1989, 2050, 2967, 3633
AsuC2I CCSGG 1 cut(s) 4026
AsuHPI GGTGA 8 cut(s) 1082, 1673, 3008, 3703, 3821, 3977, 4025, 4208
AvaI CYCGRG 3 cut(s) 3677, 4980, 5200
AvaII GGWCC 7 cut(s) 154, 876, 1798, 1892, 3130, 4171, 4564
BaeGI GKGCMC 5 cut(s) 48, 664, 1129, 2797, 4833
BaeI ACNNNNGTAYC 2 cut(s) 4410, 4443
BalI TGGCCA 1 cut(s) 2826
BanI GGYRCC 1 cut(s) 43
BanII GRGCYC 4 cut(s) 175, 1735, 3930, 4126
BauI CACGAG 2 cut(s) 135, 3039
BbsI GAAGAC 2 cut(s) 3504, 5185
Bbv12I GWGCWC 7 cut(s) 175, 664, 1129, 1735, 2956, 3930, 4833
BceAI ACGGC 7 cut(s) 324, 816, 1096, 3020, 3618, 4605, 5066
BciT130I CCWGG 6 cut(s) 323, 444, 567, 696, 2444, 3411
BciVI GTATCC 6 cut(s) 402, 2440, 2449, 3734, 4429, 5005
BclI TGATCA 5 cut(s) 354, 504, 1273, 1813, 5193
BcnI CCSGG 1 cut(s) 4026
BcoDI GTCTC 8 cut(s) 615, 1253, 1305, 1467, 1691, 2813, 4363, 5092
BfaI CTAG 8 cut(s) 525, 1920, 3089, 3368, 3993, 4482, 4782, 4826
BfmI CTRYAG 2 cut(s) 3987, 5048
BfoI RGCGCY 1 cut(s) 3634
BfuAI ACCTGC 4 cut(s) 31, 1786, 4182, 4631
BfuI GTATCC 6 cut(s) 402, 2440, 2449, 3734, 4429, 5005
BglII AGATCT 2 cut(s) 195, 5023
BlpI GCTNAGC 1 cut(s) 2640
Bme1390I CCNGG 7 cut(s) 323, 444, 567, 696, 2444, 3411, 4026
Bme18I GGWCC 7 cut(s) 154, 876, 1798, 1892, 3130, 4171, 4564
BmeT110I CYCGRG 3 cut(s) 3677, 4980, 5200
BmiI GGNNCC 8 cut(s) 45, 156, 2725, 3131, 3292, 3408, 3607, 4593
BmrFI CCNGG 7 cut(s) 323, 444, 567, 696, 2444, 3411, 4026
BmrI ACTGGG 1 cut(s) 2159
BmsI GCATC 7 cut(s) 1539, 1684, 2661, 4142, 4753, 4844, 4934
BmuI ACTGGG 1 cut(s) 2159
BoxI GACNNNNGTC 1 cut(s) 4404
BpiI GAAGAC 2 cut(s) 3504, 5185
BplI GAGNNNNNCTC 6 cut(s) 1582, 1614, 2354, 2386, 3690, 3722
BpmI CTGGAG 6 cut(s) 657, 2292, 2763, 3432, 3717, 4950
Bpu10I CCTNAGC 4 cut(s) 159, 3893, 3978, 4805
Bpu1102I GCTNAGC 1 cut(s) 2640
BpuEI CTTGAG 2 cut(s) 2681, 4481
BpuMI CCSGG 1 cut(s) 4026
BsaBI GATNNNNATC 3 cut(s) 987, 1047, 4722
BsaHI GRCGYC 1 cut(s) 1765
BsaI GGTCTC 1 cut(s) 2813
BsaWI WCCGGW 1 cut(s) 3835
BsaXI ACNNNNNCTCC 8 cut(s) 269, 299, 1186, 1216, 1233, 1263, 4923, 4953
Bse118I RCCGGY 1 cut(s) 1129
Bse1I ACTGG 7 cut(s) 517, 976, 2165, 2309, 3700, 4025, 4430
Bse3DI GCAATG 5 cut(s) 2865, 2877, 3894, 4749, 5236
Bse8I GATNNNNATC 3 cut(s) 987, 1047, 4722
BseAI TCCGGA 1 cut(s) 3835
BseBI CCWGG 6 cut(s) 323, 444, 567, 696, 2444, 3411
BseGI GGATG 7 cut(s) 1675, 1933, 3832, 4075, 4711, 4741, 4745
BseJI GATNNNNATC 3 cut(s) 987, 1047, 4722
BseMI GCAATG 5 cut(s) 2865, 2877, 3894, 4749, 5236
BseNI ACTGG 7 cut(s) 517, 976, 2165, 2309, 3700, 4025, 4430
BseRI GAGGAG 5 cut(s) 290, 357, 1621, 2591, 5056
BseSI GKGCMC 5 cut(s) 48, 664, 1129, 2797, 4833
BseYI CCCAGC 2 cut(s) 228, 1428
BsgI GTGCAG 3 cut(s) 2231, 2919, 4836
Bsh1236I CGCG 1 cut(s) 4163
BshFI GGCC 7 cut(s) 130, 1057, 2826, 3186, 3293, 3676, 4024
BshNI GGYRCC 1 cut(s) 43
BsiHKAI GWGCWC 7 cut(s) 175, 664, 1129, 1735, 2956, 3930, 4833
BsiHKCI CYCGRG 3 cut(s) 3677, 4980, 5200
BsiSI CCGG 4 cut(s) 879, 1130, 3836, 4026
BslFI GGGAC 5 cut(s) 39, 1627, 3079, 4902, 5153
BsmAI GTCTC 8 cut(s) 615, 1253, 1305, 1467, 1691, 2813, 4363, 5092
BsmFI GGGAC 5 cut(s) 39, 1627, 3079, 4902, 5153
BsmI GAATGC 2 cut(s) 10, 4818
BsnI GGCC 7 cut(s) 130, 1057, 2826, 3186, 3293, 3676, 4024
Bso31I GGTCTC 1 cut(s) 2813
BsoBI CYCGRG 3 cut(s) 3677, 4980, 5200
Bsp13I TCCGGA 1 cut(s) 3835
Bsp1407I TGTACA 2 cut(s) 2939, 4798
Bsp1720I GCTNAGC 1 cut(s) 2640
Bsp19I CCATGG 2 cut(s) 489, 4900
BspACI CCGC 9 cut(s) 126, 217, 794, 1054, 2333, 2927, 3272, 3475, 3689
BspANI GGCC 7 cut(s) 130, 1057, 2826, 3186, 3293, 3676, 4024
BspEI TCCGGA 1 cut(s) 3835
BspFNI CGCG 1 cut(s) 4163
BspLI GGNNCC 8 cut(s) 45, 156, 2725, 3131, 3292, 3408, 3607, 4593
BspMAI CTGCAG 2 cut(s) 3991, 5052
BspMI ACCTGC 4 cut(s) 31, 1786, 4182, 4631
BspPI GGATC 6 cut(s) 1037, 1956, 2257, 2669, 4047, 4102
BspQI GCTCTTC 2 cut(s) 2322, 5068
BspT107I GGYRCC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 2813
BsrDI GCAATG 5 cut(s) 2865, 2877, 3894, 4749, 5236
BsrFI RCCGGY 1 cut(s) 1129
BsrGI TGTACA 2 cut(s) 2939, 4798
BsrI ACTGG 7 cut(s) 517, 976, 2165, 2309, 3700, 4025, 4430
BssAI RCCGGY 1 cut(s) 1129
BssNI GRCGYC 1 cut(s) 1765
BssSI CACGAG 2 cut(s) 135, 3039
BssT1I CCWWGG 5 cut(s) 489, 847, 856, 3305, 4900
Bst2BI CACGAG 2 cut(s) 135, 3039
Bst2UI CCWGG 6 cut(s) 323, 444, 567, 696, 2444, 3411
Bst6I CTCTTC 4 cut(s) 1639, 2322, 5068, 5218
BstACI GRCGYC 1 cut(s) 1765
BstAPI GCANNNNNTGC 1 cut(s) 4305
BstAUI TGTACA 2 cut(s) 2939, 4798
BstDSI CCRYGG 4 cut(s) 489, 2706, 3510, 4900
BstEII GGTNACC 1 cut(s) 713
BstF5I GGATG 7 cut(s) 1675, 1933, 3832, 4075, 4711, 4741, 4745
BstFNI CGCG 1 cut(s) 4163
BstH2I RGCGCY 1 cut(s) 3634
BstHHI GCGC 5 cut(s) 16, 1989, 2050, 2967, 3633
BstMAI GTCTC 8 cut(s) 615, 1253, 1305, 1467, 1691, 2813, 4363, 5092
BstNI CCWGG 6 cut(s) 323, 444, 567, 696, 2444, 3411
BstPAI GACNNNNGTC 1 cut(s) 4404
BstPI GGTNACC 1 cut(s) 713
BstSCI CCNGG 7 cut(s) 321, 442, 565, 694, 2442, 3409, 4024
BstSFI CTRYAG 2 cut(s) 3987, 5048
BstSLI GKGCMC 5 cut(s) 48, 664, 1129, 2797, 4833
BstUI CGCG 1 cut(s) 4163
BstV2I GAAGAC 2 cut(s) 3504, 5185
BstX2I RGATCY 4 cut(s) 195, 2249, 4039, 5023
BstYI RGATCY 4 cut(s) 195, 2249, 4039, 5023
BsuI GTATCC 6 cut(s) 402, 2440, 2449, 3734, 4429, 5005
BsuRI GGCC 7 cut(s) 130, 1057, 2826, 3186, 3293, 3676, 4024
BtgI CCRYGG 4 cut(s) 489, 2706, 3510, 4900
BtsCI GGATG 7 cut(s) 1675, 1933, 3832, 4075, 4711, 4741, 4745
BtsI GCAGTG 2 cut(s) 3984, 4258
BtsIMutI CAGTG 9 cut(s) 463, 715, 757, 1874, 2822, 3984, 4258, 4419, 4437
BveI ACCTGC 4 cut(s) 31, 1786, 4182, 4631
CaiI CAGNNNCTG 2 cut(s) 2315, 2417
CfoI GCGC 5 cut(s) 16, 1989, 2050, 2967, 3633
Cfr10I RCCGGY 1 cut(s) 1129
CseI GACGC 5 cut(s) 350, 503, 1754, 4046, 4152
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
DraIII CACNNNGTG 3 cut(s) 137, 458, 2948
DrdI GACNNNNNNGTC 1 cut(s) 5165
DseDI GACNNNNNNGTC 1 cut(s) 5165
EaeI YGGCCR 2 cut(s) 2824, 3184
Eam1104I CTCTTC 4 cut(s) 1639, 2322, 5068, 5218
EarI CTCTTC 4 cut(s) 1639, 2322, 5068, 5218
Ecl136II GAGCTC 3 cut(s) 173, 1733, 3928
Eco130I CCWWGG 5 cut(s) 489, 847, 856, 3305, 4900
Eco24I GRGCYC 4 cut(s) 175, 1735, 3930, 4126
Eco31I GGTCTC 1 cut(s) 2813
Eco32I GATATC 1 cut(s) 602
Eco47I GGWCC 7 cut(s) 154, 876, 1798, 1892, 3130, 4171, 4564
Eco53kI GAGCTC 3 cut(s) 173, 1733, 3928
Eco57I CTGAAG 5 cut(s) 1599, 1736, 1869, 2034, 2346
Eco88I CYCGRG 3 cut(s) 3677, 4980, 5200
Eco91I GGTNACC 1 cut(s) 713
EcoICRI GAGCTC 3 cut(s) 173, 1733, 3928
EcoO109I RGGNCCY 2 cut(s) 1892, 4171
EcoO65I GGTNACC 1 cut(s) 713
EcoRI GAATTC 3 cut(s) 1165, 4469, 4905
EcoRII CCWGG 6 cut(s) 321, 442, 565, 694, 2442, 3409
EcoRV GATATC 1 cut(s) 602
EcoT14I CCWWGG 5 cut(s) 489, 847, 856, 3305, 4900
EcoT22I ATGCAT 3 cut(s) 1233, 1554, 2081
EcoT38I GRGCYC 4 cut(s) 175, 1735, 3930, 4126
ErhI CCWWGG 5 cut(s) 489, 847, 856, 3305, 4900
FaqI GGGAC 5 cut(s) 39, 1627, 3079, 4902, 5153
FauI CCCGC 1 cut(s) 119
FauNDI CATATG 2 cut(s) 18, 3715
FbaI TGATCA 5 cut(s) 354, 504, 1273, 1813, 5193
FblI GTMKAC 1 cut(s) 3450
FokI GGATG 7 cut(s) 1662, 1920, 3819, 4082, 4718, 4728, 4732
FriOI GRGCYC 4 cut(s) 175, 1735, 3930, 4126
FspBI CTAG 8 cut(s) 525, 1920, 3089, 3368, 3993, 4482, 4782, 4826
GlaI GCGC 5 cut(s) 15, 1988, 2049, 2966, 3632
GsaI CCCAGC 2 cut(s) 232, 1432
GsuI CTGGAG 6 cut(s) 657, 2292, 2763, 3432, 3717, 4950
HaeII RGCGCY 1 cut(s) 3634
HaeIII GGCC 7 cut(s) 130, 1057, 2826, 3186, 3293, 3676, 4024
HapII CCGG 4 cut(s) 879, 1130, 3836, 4026
HgaI GACGC 5 cut(s) 350, 503, 1754, 4046, 4152
HhaI GCGC 5 cut(s) 16, 1989, 2050, 2967, 3633
Hin1I GRCGYC 1 cut(s) 1765
Hin6I GCGC 5 cut(s) 14, 1987, 2048, 2965, 3631
HinP1I GCGC 5 cut(s) 14, 1987, 2048, 2965, 3631
HincII GTYRAC 4 cut(s) 301, 2275, 2763, 3451
HindII GTYRAC 4 cut(s) 301, 2275, 2763, 3451
HindIII AAGCTT 4 cut(s) 2523, 2909, 3150, 3866
HpaII CCGG 4 cut(s) 879, 1130, 3836, 4026
HphI GGTGA 8 cut(s) 1082, 1673, 3008, 3703, 3821, 3977, 4025, 4208
Hpy99I CGWCG 1 cut(s) 655
HpyAV CCTTC 9 cut(s) 1883, 2009, 2557, 3554, 3799, 3962, 4210, 4435, 4696
HpyCH4IV ACGT 2 cut(s) 2915, 4288
HpySE526I ACGT 2 cut(s) 2915, 4288
Hsp92I GRCGYC 1 cut(s) 1765
HspAI GCGC 5 cut(s) 14, 1987, 2048, 2965, 3631
Kpn2I TCCGGA 1 cut(s) 3835
Ksp22I TGATCA 5 cut(s) 354, 504, 1273, 1813, 5193
LguI GCTCTTC 2 cut(s) 2322, 5068
LmnI GCTCC 6 cut(s) 1326, 1730, 3413, 3605, 4182, 4597
LweI GCATC 7 cut(s) 1539, 1684, 2661, 4142, 4753, 4844, 4934
MaeI CTAG 8 cut(s) 525, 1920, 3089, 3368, 3993, 4482, 4782, 4826
MaeII ACGT 2 cut(s) 2915, 4288
MfeI CAATTG 5 cut(s) 957, 1277, 1685, 1772, 4870
MflI RGATCY 4 cut(s) 195, 2249, 4039, 5023
MlsI TGGCCA 1 cut(s) 2826
MluI ACGCGT 1 cut(s) 4161
MluNI TGGCCA 1 cut(s) 2826
MlyI GAGTC 4 cut(s) 1693, 1795, 4507, 5153
MmeI TCCRAC 3 cut(s) 1524, 1708, 4610
Mox20I TGGCCA 1 cut(s) 2826
Mph1103I ATGCAT 3 cut(s) 1233, 1554, 2081
MroI TCCGGA 1 cut(s) 3835
MroXI GAANNNNTTC 1 cut(s) 3489
MscI TGGCCA 1 cut(s) 2826
MslI CAYNNNNRTG 3 cut(s) 2089, 4263, 4840
Msp20I TGGCCA 1 cut(s) 2826
MspA1I CMGCKG 6 cut(s) 217, 296, 1054, 2720, 3475, 5222
MspI CCGG 4 cut(s) 879, 1130, 3836, 4026
MspR9I CCNGG 7 cut(s) 323, 444, 567, 696, 2444, 3411, 4026
MunI CAATTG 5 cut(s) 957, 1277, 1685, 1772, 4870
Mva1269I GAATGC 2 cut(s) 10, 4818
MvaI CCWGG 6 cut(s) 323, 444, 567, 696, 2444, 3411
MvnI CGCG 1 cut(s) 4163
NciI CCSGG 1 cut(s) 4026
NcoI CCATGG 2 cut(s) 489, 4900
NdeI CATATG 2 cut(s) 18, 3715
NlaIV GGNNCC 8 cut(s) 45, 156, 2725, 3131, 3292, 3408, 3607, 4593
NmeAIII GCCGAG 2 cut(s) 4060, 5137
NsiI ATGCAT 3 cut(s) 1233, 1554, 2081
OliI CACNNNNGTG 1 cut(s) 4263
PaeR7I CTCGAG 2 cut(s) 4980, 5200
PaqCI CACCTGC 1 cut(s) 31
PciI ACATGT 3 cut(s) 558, 645, 1803
PciSI GCTCTTC 2 cut(s) 2322, 5068
PcsI WCGNNNNNNNCGW 1 cut(s) 434
PctI GAATGC 2 cut(s) 10, 4818
PdmI GAANNNNTTC 1 cut(s) 3489
PflMI CCANNNNNTGG 2 cut(s) 572, 2860
PfoI TCCNGGA 1 cut(s) 3409
PleI GAGTC 4 cut(s) 1693, 1794, 4506, 5153
PpsI GAGTC 4 cut(s) 1693, 1794, 4506, 5153
PpuMI RGGWCCY 2 cut(s) 1892, 4171
PscI ACATGT 3 cut(s) 558, 645, 1803
PshAI GACNNNNGTC 1 cut(s) 4404
PsiI TTATAA 2 cut(s) 1211, 2619
Psp124BI GAGCTC 3 cut(s) 175, 1735, 3930
Psp1406I AACGTT 1 cut(s) 4288
Psp5II RGGWCCY 2 cut(s) 1892, 4171
Psp6I CCWGG 6 cut(s) 321, 442, 565, 694, 2442, 3409
PspEI GGTNACC 1 cut(s) 713
PspFI CCCAGC 2 cut(s) 228, 1428
PspGI CCWGG 6 cut(s) 321, 442, 565, 694, 2442, 3409
PspN4I GGNNCC 8 cut(s) 45, 156, 2725, 3131, 3292, 3408, 3607, 4593
PspPPI RGGWCCY 2 cut(s) 1892, 4171
PspXI VCTCGAGB 2 cut(s) 4980, 5200
PstI CTGCAG 2 cut(s) 3991, 5052
PstNI CAGNNNCTG 2 cut(s) 2315, 2417
PsuI RGATCY 4 cut(s) 195, 2249, 4039, 5023
PvuII CAGCTG 3 cut(s) 296, 2720, 5222
RseI CAYNNNNRTG 3 cut(s) 2089, 4263, 4840
SacI GAGCTC 3 cut(s) 175, 1735, 3930
SalI GTCGAC 1 cut(s) 3449
SapI GCTCTTC 2 cut(s) 2322, 5068
SchI GAGTC 4 cut(s) 1693, 1795, 4507, 5153
ScrFI CCNGG 7 cut(s) 323, 444, 567, 696, 2444, 3411, 4026
SfaNI GCATC 7 cut(s) 1539, 1684, 2661, 4142, 4753, 4844, 4934
SfcI CTRYAG 2 cut(s) 3987, 5048
Sfr274I CTCGAG 2 cut(s) 4980, 5200
SinI GGWCC 7 cut(s) 154, 876, 1798, 1892, 3130, 4171, 4564
SlaI CTCGAG 2 cut(s) 4980, 5200
SmiMI CAYNNNNRTG 3 cut(s) 2089, 4263, 4840
SmlI CTYRAG 4 cut(s) 2660, 4460, 4980, 5200
SmoI CTYRAG 4 cut(s) 2660, 4460, 4980, 5200
SsiI CCGC 9 cut(s) 126, 217, 794, 1054, 2333, 2927, 3272, 3475, 3689
SspI AATATT 2 cut(s) 3489, 3752
SspMI CTAG 8 cut(s) 525, 1920, 3089, 3368, 3993, 4482, 4782, 4826
SstI GAGCTC 3 cut(s) 175, 1735, 3930
StyD4I CCNGG 7 cut(s) 321, 442, 565, 694, 2442, 3409, 4024
StyI CCWWGG 5 cut(s) 489, 847, 856, 3305, 4900
TaiI ACGT 2 cut(s) 2918, 4291
TaqI TCGA 9 cut(s) 653, 1702, 3450, 3747, 3924, 4629, 4716, 4981, 5201
TatI WGTACW 5 cut(s) 73, 2939, 3213, 4267, 4798
TauI GCSGC 3 cut(s) 220, 1057, 2335
TscAI CASTG 9 cut(s) 463, 715, 757, 1874, 2822, 3991, 4265, 4426, 4437
TspGWI ACGGA 6 cut(s) 105, 1826, 3527, 3698, 4394, 4552
TspRI CASTG 9 cut(s) 463, 715, 757, 1874, 2822, 3991, 4265, 4426, 4437
Van91I CCANNNNNTGG 2 cut(s) 572, 2860
VneI GTGCAC 3 cut(s) 660, 1125, 4829
VpaK11BI GGWCC 7 cut(s) 154, 876, 1798, 1892, 3130, 4171, 4564
XbaI TCTAGA 1 cut(s) 1919
XcmI CCANNNNNNNNNTGG 1 cut(s) 863
XhoI CTCGAG 2 cut(s) 4980, 5200
XmiI GTMKAC 1 cut(s) 3450
XmnI GAANNNNTTC 1 cut(s) 3489
XspI CTAG 8 cut(s) 525, 1920, 3089, 3368, 3993, 4482, 4782, 4826
Zsp2I ATGCAT 3 cut(s) 1233, 1554, 2081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.