Rw2G043000

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000307
Physical Location & Seq
Reverse (-)
69324937 .. 69330797
5861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043000.1

Sequence Viewer

Length: 3585 bp
ATGAACCATGTGAGATCCTCATCTTCTTCATCCCTTTCTTTATGGGAACATGATGTTTTCCTCAGTTTTAGGGGTGAGGACACCCGCAACAGCTTCACCGACCATTTATACCGTACTTTGAGTCAGCGTGGAATTAACACATTTAGGGATGCTGAAAAACTCCAGAAGGGAAATTCCGTGTTACCAACGCTCGTTGAAGCCATCCGAAGATCCAGATGTGCTGTCATTGTTCTTTCAGCAAACTATGCTTCTTCAACATGGTGTTTGAATGAGCTTGTGCATATTCTTGAGTTCAAGAAAGTGGGAGGAAGCAAACTGGAGGTGTTTCCGGTTTTCTATAACGTCGAACCGTCCGAGGTCAGAAAGCAAACTGGAGTTTACGGGGAAGCTTTGTCTGCGCATGAAGATCTGAACAAGGTGGACATATGGAAGAATGCCTTGACTGAAGTAGCGAATTTCTCCGGATGGGATGTGAAAAATTGGAGTGAATCAGAAGTTACCGAGGAAATAGCTGAGAAGATTTCAAATATATTGAAGTTCACGTTTCCAACTGCTAACGATGATCTAATTGGAATGGATTCTCGAATAGAGAAAGTGGAGTCCTACTTGGATCTAAGCCTGGATGTTGTTCGCACAATTGGGATTTCGGGAATGGGGGGCATAGGTAAAACAACTCTTGCACAAGAAGTTTTCAAGAAGATCCTTGGAAACTTTGATACTAGCGCATTTGTGGCTAATGTTAGAGAGAAATCAGGGAATGTAAATGGTCTAGTTGACTTGCAAAAATCCCTTTATAAATCCTTGCTACACAGCGATGTAGATATACATTCTGTTGACAAGGGAAAATATTTGTTAAGGAGAGGACTCCGTTCAAAGAAGGTGCTCATCATTCTAGACGATGTTCATGAACTCAAACAAATGGAAGCTCTTGCTGGAAGTAGTAAACAGAATCATTGGTTTGGTCCAGGGAGTAGAGTAATTATAACAACTAGAGATGAGCAGTTGTTGACGTCTAATGACGTGCACAAGACATACGAGGTTGAGAAACTGAATGATGCTGAAGCTTCTCAGCTATTGTGTCAGAGAGCCTTCAAGAAAAACCATGCTCCAACAGAATATCTAAAGCTGTGCAACAATTTTGTAAAATATGCCAGTGGCCTTCCCTTAGCTCTTGAAGTTTTGGGTTCTTACTTGTGCCGGAAGGAAGTAAGTGAATGGTCAGCAGCATTGGCTAGACTATATGAAGATCCTGAAAAAGACATTATGAGAGTGCTTCAATTAAGTTATAATGGATTGAAGTCAACAGTGAAGGATATGTTTCTGGACATTGCATGTTTTTTCCGAGGAGAGGAACAAGATCGTGTAATGAAGATCTTCACCAGTTGTGACTTTCATCCGGAAATCGGCATTGTTGACCTTATTGAAAAATCTTTGATTAAAATTGAAGGGAAAAAACTGTGGATGCATGATTTACTACAACAAATGGGTTGGCAGATTGTTCGTCAAGAATCTCCTAAAGAGCCGGGTAAACGAAGTAGGTTGTGGCTTGATGAGAATGCTTGTGAATACGAACGCTGGAGGTCGTGGTTTGATGAGGAACAGTCGAGCAAATGCAGAAGGTTCTGGCTTGACAAGGATGCTCGTGCTGTACTCATACAGAATCTAGGAACAGAACATGTTGAAGGTTTATTCCTAAGCTTGCAGGCAAAAGAAGAGTTACACTTGAATGCTGATCCATTCTACAAAATGCCAAACCTAAGGTTGTTAAAGATTTATAATGTCAACTTTGCTGGATGCATTGAACTTATTTCTCTCTCCCAAAATCTAAGGCTATTGGAATGGCATGAATTTCCTTTGAATTCTTTGCCATCAAGTTTTAGACCAAATGTGCTCGCTGAACTCAAGATGCCAAACAGCCGAATTACGCAACTATGGAGTGAAACTCTTTCTGCGGAAATGCTGATGCTTATGGATCTTTCAAACTGCCAATACTTGACTAGGACCCCGGATTTTAGTACAGTTCCAAAGCTTGAGAGGTTGATCCTCGAAGGTTGTAAGAGATTATCAGAGGTTCACCCAACAGTTGGAGGTCTCAAACATCTGGTCTTATTAAATTTGAAAGGTTGTGAAAGTGTAGAGAGCCTTCCTGACTCCATCAGCTTGGAATCTCTCCAGACCTTAGTCCTTTCAGGATGTTTGAAAATCAAAAAGTTTCCAGAGATTATGGGAAACATGGAAAATATGTCGGAACTTTATCTAGATGCGACGGCTATACAGGAGCTGCCAATATCGTTGAAGCACTTAAGTGGCCTTAGTTTGTTAAATCTAAGAGGCTGCAAGAACCTCTCAAGTCTTCCAAGCTTCATTTGTAGTTTGACATCTCTGATATCTCTCAATCTGTCAGGCTGTTCGCTTATAAATCAACTGCCAGAAAACCTAGGGAGCTTGGAACATTTGCAAGAGGTTGATGCCTGTGAAACTTCTATAACAAAAGTTCCTTCGTCTATTTTAGTGCTTCTGTTGAAGAACCTTAAACGATTATGTTTTCGTGGATGTAATGATTTATGGTTTCCAGCTGAGGACCCGTTCTCAGATTTAGCAGGCTTATCCCGTTCCCGTTTGACATCATTAAGTGTATCTCGTCAAGATATCTTATATATACCTGATGAGATTGGCCTCTTAAACTCACTTCAGCGTTTAGATTTGAGTGTAACTAATTTGTCTAGCATACCCGAAAGCGTCAGTGAACTTTCTAAGCTCAGAGAGCTTTGCTTGTATGGGTGTATCAGGCTTCGGTCATTACCAAAGTACCTGCCGCTGAGTCTAAAATTTATAGATGCACGTTATTGTTCTATGCTGACAAATTTTCCAAATAATTGTGCAATATGGGCTTCGGAGGAGAGTTTCAGTTTCATGGATTGCAGAAATTCAGTTGATGATATCGAAGATCATGTAAACATAAGGCGTCTTAAATACATTGAGGATCGAATCTATCACGAACAGCCGTTTGAAATCCATCTTCGTCATACTCGAATCCCAGACTGGTGTGGTTATTCTAGGAGAGGGTCTTCTGTAACAATCCCACTATCTGATCCAGAGGATGGTGATAGTACATGGATGGGATTTGTCCTTTTCGTTGCTTTCGAAATCCTAGACAATAGAAACTTTGATAAGAGTTGGGAATTGAAAGATACTTTCTGTCATTTTAGCACCTACGAAGATCCTCTTGAAAATCCCCTAGTCTTTCGAAACTTCAGTAACCCCGGGGTTGGGCCATTTGTGCTCTGTTGCTATGTGCCACAACGACTGTTCTCAAGGAAGTTGAATAGAGCAAGTCGTCTACTCAGAGCTTCCATTTCAACGGAGAGACCGGATTTGGAAGTGAAAGAGTGTGGAATACAACTAATATCCCAGCAAGGTGCTGCATTGTCTGTTCAAGATTTAACACAGACAGCTAGTGATCAACTTCATCAGCTGGATTCAAATTTTGTTCCTCCACACATCGTAACGGAGGAAGTGCAGCAGAGAAGCCATCATTTACAGCTGATACTTTCAAAACTCTATGAGGCTATGTATGTGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1194

Amino Acids

136.01

Weight (kDa)

5.89

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 186 1.2e-49 TIR domain
TIR_2 PF13676 19 - 143 6.1e-16 TIR domain
NB-ARC PF00931 195 - 367 1.3e-23 NB-ARC domain
NACHT PF05729 211 - 361 1.2e-06 NACHT domain
WHD_ROQ1 PF23282 437 - 504 7.8e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 721 - 954 1e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 874 - 931 3.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 795, 983, 1287, 1776, 2417
AatII GACGTC 1 cut(s) 1013
Acc16I TGCGCA 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 2820
AccB7I CCANNNNNTGG 2 cut(s) 2084, 3101
AccI GTMKAC 1 cut(s) 3340
AccIII TCCGGA 2 cut(s) 461, 1396
AciI CCGC 3 cut(s) 85, 1952, 2816
AcsI RAATTY 9 cut(s) 172, 454, 1847, 1858, 2113, 2828, 2863, 2926, 3484
AcuI CTGAAG 4 cut(s) 465, 1080, 2675, 3238
AcyI GRCGYC 2 cut(s) 1010, 2965
AfaI GTAC 5 cut(s) 115, 1650, 2017, 2810, 3112
AfiI CCNNNNNNNGG 6 cut(s) 1348, 1403, 2084, 3101, 3269, 3270
AflII CTTAAG 1 cut(s) 2302
AflIII ACRYGT 1 cut(s) 1675
AjiI CACGTC 1 cut(s) 1021
AjnI CCWGG 2 cut(s) 618, 964
AleI CACNNNNGTG 1 cut(s) 2304
Alw21I GWGCWC 4 cut(s) 885, 1026, 1893, 3285
Alw26I GTCTC 2 cut(s) 2096, 3361
Alw44I GTGCAC 1 cut(s) 1022
AlwNI CAGNNNCTG 1 cut(s) 2281
Ama87I CYCGRG 1 cut(s) 3263
Aor13HI TCCGGA 2 cut(s) 461, 1396
AoxI GGCC 4 cut(s) 1156, 2308, 2674, 3272
ApaLI GTGCAC 1 cut(s) 1022
ApeKI GCWGC 5 cut(s) 1223, 2281, 2334, 3422, 3520
ApoI RAATTY 9 cut(s) 172, 454, 1847, 1858, 2113, 2828, 2863, 2926, 3484
ArsI GACNNNNNNTTYG 2 cut(s) 2088, 2120
Asp700I GAANNNNTTC 2 cut(s) 577, 1373
AspA2I CCTAGG 1 cut(s) 2437
AspLEI GCGC 2 cut(s) 400, 725
AspS9I GGNCC 4 cut(s) 962, 2001, 2581, 3272
AsuC2I CCSGG 4 cut(s) 1524, 2006, 3264, 3265
AsuHPI GGTGA 5 cut(s) 86, 88, 1369, 2066, 3116
AsuII TTCGAA 2 cut(s) 3144, 3247
AvaI CYCGRG 1 cut(s) 3263
AvaII GGWCC 3 cut(s) 962, 2001, 2581
AvrII CCTAGG 1 cut(s) 2437
AxyI CCTNAGG 1 cut(s) 1757
BaeGI GKGCMC 1 cut(s) 1026
BaeI ACNNNNGTAYC 2 cut(s) 2792, 2825
BarI GAAGNNNNNNTAC 2 cut(s) 2127, 2159
BauI CACGAG 1 cut(s) 1641
BbsI GAAGAC 2 cut(s) 2345, 3060
Bbv12I GWGCWC 4 cut(s) 885, 1026, 1893, 3285
BbvCI CCTCAGC 1 cut(s) 2577
BbvI GCAGC 5 cut(s) 1235, 2268, 2321, 3409, 3532
BccI CCATC 8 cut(s) 209, 459, 1876, 2162, 3024, 3095, 3112, 3540
BceAI ACGGC 2 cut(s) 2283, 2989
BcgI CGANNNNNNTGC 2 cut(s) 1481, 1515
BciT130I CCWGG 2 cut(s) 620, 966
BclI TGATCA 1 cut(s) 3460
BcnI CCSGG 4 cut(s) 1524, 2006, 3264, 3265
BcoDI GTCTC 2 cut(s) 2096, 3361
BfrI CTTAAG 1 cut(s) 2302
BfuAI ACCTGC 1 cut(s) 2820
BglII AGATCT 2 cut(s) 406, 1371
BisI GCNGC 6 cut(s) 1224, 2282, 2335, 2816, 3423, 3521
BlnI CCTAGG 1 cut(s) 2437
BlsI GCNGC 6 cut(s) 1225, 2283, 2336, 2817, 3424, 3522
Bme1390I CCNGG 6 cut(s) 620, 966, 1524, 2006, 3264, 3265
Bme18I GGWCC 3 cut(s) 962, 2001, 2581
BmeT110I CYCGRG 1 cut(s) 3263
BmgBI CACGTC 1 cut(s) 1021
BmgT120I GGNCC 4 cut(s) 962, 2001, 2581, 3272
BmiI GGNNCC 2 cut(s) 2003, 2583
BmrFI CCNGG 6 cut(s) 620, 966, 1524, 2006, 3264, 3265
BoxI GACNNNNGTC 1 cut(s) 2180
BpiI GAAGAC 2 cut(s) 2345, 3060
BplI GAGNNNNNCTC 2 cut(s) 1927, 1959
BpmI CTGGAG 5 cut(s) 146, 338, 393, 1597, 2156
Bpu10I CCTNAGC 3 cut(s) 1165, 1694, 2577
Bpu14I TTCGAA 2 cut(s) 3144, 3247
BpuEI CTTGAG 5 cut(s) 308, 1886, 2051, 2332, 3298
BpuMI CCSGG 4 cut(s) 1524, 2006, 3264, 3265
BsaBI GATNNNNATC 1 cut(s) 19
BsaHI GRCGYC 2 cut(s) 1010, 2965
BsaI GGTCTC 2 cut(s) 2096, 3361
BsaWI WCCGGW 4 cut(s) 328, 461, 1396, 3370
Bsc4I CCNNNNNNNGG 6 cut(s) 1348, 1403, 2084, 3101, 3269, 3270
Bse1I ACTGG 5 cut(s) 321, 376, 1152, 1380, 3047
Bse21I CCTNAGG 1 cut(s) 1757
Bse3DI GCAATG 1 cut(s) 1326
Bse8I GATNNNNATC 1 cut(s) 19
BseAI TCCGGA 2 cut(s) 461, 1396
BseBI CCWGG 2 cut(s) 620, 966
BseJI GATNNNNATC 1 cut(s) 19
BseLI CCNNNNNNNGG 6 cut(s) 1348, 1403, 2084, 3101, 3269, 3270
BseMI GCAATG 1 cut(s) 1326
BseMII CTCAG 8 cut(s) 76, 504, 1082, 2568, 2604, 2773, 2810, 3358
BseNI ACTGG 5 cut(s) 321, 376, 1152, 1380, 3047
BseRI GAGGAG 2 cut(s) 1359, 2912
BseSI GKGCMC 1 cut(s) 1026
BseXI GCAGC 5 cut(s) 1235, 2268, 2321, 3409, 3532
BseYI CCCAGC 1 cut(s) 3411
BsgI GTGCAG 1 cut(s) 3539
BshFI GGCC 4 cut(s) 1158, 2310, 2676, 3274
BsiHKAI GWGCWC 4 cut(s) 885, 1026, 1893, 3285
BsiHKCI CYCGRG 1 cut(s) 3263
BsiSI CCGG 8 cut(s) 329, 462, 1198, 1397, 1523, 2006, 3264, 3371
BslI CCNNNNNNNGG 6 cut(s) 1348, 1403, 2084, 3101, 3269, 3270
BsmAI GTCTC 2 cut(s) 2096, 3361
BsmI GAATGC 3 cut(s) 439, 1561, 1732
BsnI GGCC 4 cut(s) 1158, 2310, 2676, 3274
Bso31I GGTCTC 2 cut(s) 2096, 3361
BsoBI CYCGRG 1 cut(s) 3263
Bsp119I TTCGAA 2 cut(s) 3144, 3247
Bsp1286I GDGCHC 4 cut(s) 885, 1026, 1893, 3285
Bsp13I TCCGGA 2 cut(s) 461, 1396
BspACI CCGC 3 cut(s) 85, 1952, 2816
BspANI GGCC 4 cut(s) 1158, 2310, 2676, 3274
BspCNI CTCAG 8 cut(s) 75, 505, 1081, 2569, 2603, 2772, 2811, 3357
BspEI TCCGGA 2 cut(s) 461, 1396
BspHI TCATGA 1 cut(s) 904
BspLI GGNNCC 2 cut(s) 2003, 2583
BspMI ACCTGC 1 cut(s) 2820
BspT104I TTCGAA 2 cut(s) 3144, 3247
BspTI CTTAAG 1 cut(s) 2302
BspTNI GGTCTC 2 cut(s) 2096, 3361
BsrDI GCAATG 1 cut(s) 1326
BsrI ACTGG 5 cut(s) 321, 376, 1152, 1380, 3047
BssNI GRCGYC 2 cut(s) 1010, 2965
BssSI CACGAG 1 cut(s) 1641
BssT1I CCWWGG 2 cut(s) 703, 2437
Bst2BI CACGAG 1 cut(s) 1641
Bst2UI CCWGG 2 cut(s) 620, 966
Bst4CI ACNGT 8 cut(s) 113, 351, 1306, 1458, 1602, 2020, 2083, 3309
Bst6I CTCTTC 1 cut(s) 1707
BstACI GRCGYC 2 cut(s) 1010, 2965
BstAFI CTTAAG 1 cut(s) 2302
BstAPI GCANNNNNTGC 1 cut(s) 245
BstBI TTCGAA 2 cut(s) 3144, 3247
BstC8I GCNNGC 4 cut(s) 1700, 1704, 1893, 2602
BstHHI GCGC 2 cut(s) 400, 725
BstMAI GTCTC 2 cut(s) 2096, 3361
BstMWI GCNNNNNNNGC 7 cut(s) 245, 395, 731, 1229, 2764, 2888, 3280
BstNI CCWGG 2 cut(s) 620, 966
BstNSI RCATGY 2 cut(s) 1335, 1679
BstPAI GACNNNNGTC 1 cut(s) 2180
BstSCI CCNGG 6 cut(s) 618, 964, 1522, 2004, 3262, 3263
BstSLI GKGCMC 1 cut(s) 1026
BstV1I GCAGC 5 cut(s) 1235, 2268, 2321, 3409, 3532
BstV2I GAAGAC 2 cut(s) 2345, 3060
BstX2I RGATCY 9 cut(s) 14, 209, 406, 610, 699, 1246, 1371, 1972, 3220
BstXI CCANNNNNNTGG 1 cut(s) 2161
BstYI RGATCY 9 cut(s) 14, 209, 406, 610, 699, 1246, 1371, 1972, 3220
Bsu36I CCTNAGG 1 cut(s) 1757
BsuRI GGCC 4 cut(s) 1158, 2310, 2676, 3274
BtgZI GCGATG 1 cut(s) 828
BtrI CACGTC 1 cut(s) 1021
BtsIMutI CAGTG 3 cut(s) 1159, 1311, 2749
BveI ACCTGC 1 cut(s) 2820
Cac8I GCNNGC 4 cut(s) 1700, 1704, 1893, 2602
CaiI CAGNNNCTG 1 cut(s) 2281
CciI TCATGA 1 cut(s) 904
CfoI GCGC 2 cut(s) 400, 725
Cfr13I GGNCC 4 cut(s) 962, 2001, 2581, 3272
Cfr9I CCCGGG 1 cut(s) 3263
CseI GACGC 2 cut(s) 2728, 2954
Csp6I GTAC 5 cut(s) 114, 1649, 2016, 2809, 3111
CviQI GTAC 5 cut(s) 114, 1649, 2016, 2809, 3111
Eam1104I CTCTTC 1 cut(s) 1707
EarI CTCTTC 1 cut(s) 1707
Eco130I CCWWGG 2 cut(s) 703, 2437
Eco31I GGTCTC 2 cut(s) 2096, 3361
Eco32I GATATC 3 cut(s) 2388, 2650, 2941
Eco47I GGWCC 3 cut(s) 962, 2001, 2581
Eco57I CTGAAG 4 cut(s) 465, 1080, 2675, 3238
Eco81I CCTNAGG 1 cut(s) 1757
Eco88I CYCGRG 1 cut(s) 3263
EcoO109I RGGNCCY 2 cut(s) 2001, 2581
EcoRI GAATTC 1 cut(s) 1858
EcoRII CCWGG 2 cut(s) 618, 964
EcoRV GATATC 3 cut(s) 2388, 2650, 2941
EcoT14I CCWWGG 2 cut(s) 703, 2437
EcoT22I ATGCAT 2 cut(s) 1467, 1799
ErhI CCWWGG 2 cut(s) 703, 2437
FalI AAGNNNNNCTT 4 cut(s) 422, 454, 3210, 3242
FauI CCCGC 1 cut(s) 92
FauNDI CATATG 1 cut(s) 425
FbaI TGATCA 1 cut(s) 3460
FblI GTMKAC 1 cut(s) 3340
Fnu4HI GCNGC 6 cut(s) 1224, 2282, 2335, 2816, 3423, 3521
Fsp4HI GCNGC 6 cut(s) 1224, 2282, 2335, 2816, 3423, 3521
FspI TGCGCA 1 cut(s) 399
GlaI GCGC 2 cut(s) 399, 724
GluI GCNGC 6 cut(s) 1224, 2282, 2335, 2816, 3423, 3521
GsaI CCCAGC 1 cut(s) 3415
GsuI CTGGAG 5 cut(s) 146, 338, 393, 1597, 2156
HaeIII GGCC 4 cut(s) 1158, 2310, 2676, 3274
HapII CCGG 8 cut(s) 329, 462, 1198, 1397, 1523, 2006, 3264, 3371
HgaI GACGC 2 cut(s) 2728, 2954
HhaI GCGC 2 cut(s) 400, 725
Hin1I GRCGYC 2 cut(s) 1010, 2965
Hin6I GCGC 2 cut(s) 398, 723
HinP1I GCGC 2 cut(s) 398, 723
HincII GTYRAC 6 cut(s) 775, 835, 1008, 1302, 1414, 1783
HindII GTYRAC 6 cut(s) 775, 835, 1008, 1302, 1414, 1783
HindIII AAGCTT 5 cut(s) 387, 1062, 1696, 2027, 2359
HpaII CCGG 8 cut(s) 329, 462, 1198, 1397, 1523, 2006, 3264, 3371
HphI GGTGA 5 cut(s) 86, 88, 1369, 2066, 3116
Hpy99I CGWCG 2 cut(s) 347, 2269
HpyCH4III ACNGT 8 cut(s) 113, 351, 1306, 1458, 1602, 2020, 2083, 3309
HpyCH4IV ACGT 5 cut(s) 342, 542, 1010, 1020, 2842
HpyF10VI GCNNNNNNNGC 7 cut(s) 245, 395, 731, 1229, 2764, 2888, 3280
HpySE526I ACGT 5 cut(s) 342, 542, 1010, 1020, 2842
Hsp92I GRCGYC 2 cut(s) 1010, 2965
HspAI GCGC 2 cut(s) 398, 723
Kpn2I TCCGGA 2 cut(s) 461, 1396
Ksp22I TGATCA 1 cut(s) 3460
LmnI GCTCC 3 cut(s) 1111, 2278, 2442
Lsp1109I GCAGC 5 cut(s) 1235, 2268, 2321, 3409, 3532
MaeII ACGT 5 cut(s) 342, 542, 1010, 1020, 2842
MaeIII GTNAC 8 cut(s) 180, 496, 1385, 1716, 2710, 3073, 3257, 3505
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 9 cut(s) 14, 209, 406, 610, 699, 1246, 1371, 1972, 3220
MhlI GDGCHC 4 cut(s) 885, 1026, 1893, 3285
MlyI GAGTC 5 cut(s) 130, 608, 858, 2144, 2830
MmeI TCCRAC 4 cut(s) 572, 1133, 2065, 2226
Mph1103I ATGCAT 2 cut(s) 1467, 1799
MroI TCCGGA 2 cut(s) 461, 1396
MroXI GAANNNNTTC 2 cut(s) 577, 1373
MslI CAYNNNNRTG 3 cut(s) 813, 1725, 2304
MspA1I CMGCKG 4 cut(s) 2576, 2818, 3475, 3544
MspCI CTTAAG 1 cut(s) 2302
MspI CCGG 8 cut(s) 329, 462, 1198, 1397, 1523, 2006, 3264, 3371
MspR9I CCNGG 6 cut(s) 620, 966, 1524, 2006, 3264, 3265
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 3 cut(s) 439, 1561, 1732
MvaI CCWGG 2 cut(s) 620, 966
MwoI GCNNNNNNNGC 7 cut(s) 245, 395, 731, 1229, 2764, 2888, 3280
NciI CCSGG 4 cut(s) 1524, 2006, 3264, 3265
NdeI CATATG 1 cut(s) 425
NlaIV GGNNCC 2 cut(s) 2003, 2583
NmuCI GTSAC 1 cut(s) 1385
NsbI TGCGCA 1 cut(s) 399
NsiI ATGCAT 2 cut(s) 1467, 1799
NspI RCATGY 2 cut(s) 1335, 1679
NspV TTCGAA 2 cut(s) 3144, 3247
OliI CACNNNNGTG 1 cut(s) 2304
PagI TCATGA 1 cut(s) 904
PciI ACATGT 1 cut(s) 1675
PcsI WCGNNNNNNNCGW 3 cut(s) 351, 3028, 3141
PctI GAATGC 3 cut(s) 439, 1561, 1732
PdmI GAANNNNTTC 2 cut(s) 577, 1373
PfeI GAWTC 9 cut(s) 488, 578, 949, 1508, 1660, 2165, 2988, 3033, 3479
PflMI CCANNNNNTGG 2 cut(s) 2084, 3101
PkrI GCNGC 6 cut(s) 1225, 2283, 2336, 2817, 3424, 3522
PleI GAGTC 5 cut(s) 129, 607, 858, 2144, 2829
PpsI GAGTC 5 cut(s) 129, 607, 858, 2144, 2829
PpuMI RGGWCCY 2 cut(s) 2001, 2581
PscI ACATGT 1 cut(s) 1675
PshAI GACNNNNGTC 1 cut(s) 2180
PsiI TTATAA 5 cut(s) 795, 983, 1287, 1776, 2417
Psp5II RGGWCCY 2 cut(s) 2001, 2581
Psp6I CCWGG 2 cut(s) 618, 964
PspFI CCCAGC 1 cut(s) 3411
PspGI CCWGG 2 cut(s) 618, 964
PspN4I GGNNCC 2 cut(s) 2003, 2583
PspPI GGNCC 4 cut(s) 962, 2001, 2581, 3272
PspPPI RGGWCCY 2 cut(s) 2001, 2581
PstNI CAGNNNCTG 1 cut(s) 2281
PsuI RGATCY 9 cut(s) 14, 209, 406, 610, 699, 1246, 1371, 1972, 3220
PvuII CAGCTG 3 cut(s) 2576, 3475, 3544
RsaI GTAC 5 cut(s) 115, 1650, 2017, 2810, 3112
RsaNI GTAC 5 cut(s) 114, 1649, 2016, 2809, 3111
RseI CAYNNNNRTG 3 cut(s) 813, 1725, 2304
SatI GCNGC 6 cut(s) 1224, 2282, 2335, 2816, 3423, 3521
Sau96I GGNCC 4 cut(s) 962, 2001, 2581, 3272
SchI GAGTC 5 cut(s) 130, 608, 858, 2144, 2830
ScrFI CCNGG 6 cut(s) 620, 966, 1524, 2006, 3264, 3265
SduI GDGCHC 4 cut(s) 885, 1026, 1893, 3285
SfuI TTCGAA 2 cut(s) 3144, 3247
SinI GGWCC 3 cut(s) 962, 2001, 2581
SmaI CCCGGG 1 cut(s) 3265
SmiMI CAYNNNNRTG 3 cut(s) 813, 1725, 2304
SmlI CTYRAG 6 cut(s) 287, 1901, 2030, 2302, 2347, 3313
SmoI CTYRAG 6 cut(s) 287, 1901, 2030, 2302, 2347, 3313
SsiI CCGC 3 cut(s) 85, 1952, 2816
SspI AATATT 1 cut(s) 848
StyD4I CCNGG 6 cut(s) 618, 964, 1522, 2004, 3262, 3263
StyI CCWWGG 2 cut(s) 703, 2437
TaaI ACNGT 8 cut(s) 113, 351, 1306, 1458, 1602, 2020, 2083, 3309
TaiI ACGT 5 cut(s) 345, 545, 1013, 1023, 2845
TaqI TCGA 9 cut(s) 345, 583, 1604, 2046, 2943, 2986, 3031, 3144, 3247
TaqII GACCGA 1 cut(s) 2784
TatI WGTACW 3 cut(s) 1648, 2015, 3110
TauI GCSGC 1 cut(s) 2818
TfiI GAWTC 9 cut(s) 488, 578, 949, 1508, 1660, 2165, 2988, 3033, 3479
TscAI CASTG 3 cut(s) 1159, 1311, 2749
TseFI GTSAC 1 cut(s) 1385
TseI GCWGC 5 cut(s) 1223, 2281, 2334, 3422, 3520
Tsp45I GTSAC 1 cut(s) 1385
TspGWI ACGGA 4 cut(s) 166, 857, 3377, 3524
TspMI CCCGGG 1 cut(s) 3263
TspRI CASTG 3 cut(s) 1159, 1311, 2749
Van91I CCANNNNNTGG 2 cut(s) 2084, 3101
Vha464I CTTAAG 1 cut(s) 2302
VneI GTGCAC 1 cut(s) 1022
VpaK11BI GGWCC 3 cut(s) 962, 2001, 2581
XapI RAATTY 9 cut(s) 172, 454, 1847, 1858, 2113, 2828, 2863, 2926, 3484
XbaI TCTAGA 2 cut(s) 892, 2257
XceI RCATGY 2 cut(s) 1335, 1679
XmaI CCCGGG 1 cut(s) 3263
XmaJI CCTAGG 1 cut(s) 2437
XmiI GTMKAC 1 cut(s) 3340
XmnI GAANNNNTTC 2 cut(s) 577, 1373
ZraI GACGTC 1 cut(s) 1011
Zsp2I ATGCAT 2 cut(s) 1467, 1799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.