RLG00000017516

positive regulation of cell-substrate adhesion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000307
Physical Location & Seq
Reverse (-)
17739495 .. 17749165
9671 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017516

Sequence Viewer

Length: 6438 bp
ATGCTCAGCTCGAATTTGGCTTCAATAGAGGTTGATAAGCTAATCCCTTACTCCAATTTTGCTGCTTTCTTGTTTCCCAAAACTAGCCCATCTAGTCTCCTGTGCGCATCGCCGTTTGGTCAGCGTCTCCGGCACCGATCTTCTTCCTCGTCGGAGCTCTTCCAGGTGAATTTCTTATATCAACTAAAAGGACGTAGTCCTGCTGCTGAGTCTCCAGCGGATTCCACTTGCACCCCAATACCACCTTCAACTCATATACCGGAACATGAGAAAGTTCCAGCACTCGATTCCACCAACATCCACTTTGGCCATTCATCATCATCTTCTTCCTTTGGTTTCGAACCTGATCAGGAACTTCCATCAAATTCCACCGACACACAGCTAGTACCTTCATCATCTAGATCTTCTTCCATCTTTAACCTGGAACATGAAGCAGTCACGGTGGATTGCCCAAACAACCAAGTAGCAACGTCATCATCTGGTTCATCCTCTAACTGGGAACATGATGTCTTCCTCAGTTTTAGGGGTGAGGACACCCGCAATAGTTTTACTGACCATTTATACTATGCTATGAACCAGAGAGGAATCGACACATTTCGAGATGCTGAAAAGCTTCAGAGGGGGAAATCCATTTCATCAGAGCTTCTCAAAGCAATCGAAGAGTCCAAGTTTGCAGTTATAGTTCTTTCAACCAACTATGCTACTTCAACTTGGTGTCTGGATGAACTTGCACATATTCTTGAATGCAAGAAACTGAGGGGACTGGAAGTTCTTCCTGTTTTTTATAATGTAGAACCATCTGAGATAAGAAAGCAAACTGGAATATTTGGCAAGGCATTTTCTAAGCATGAGACAAATTACAAGGACAATATCAAGAAGGTGAACAAGTGGAGGGAAGCTTTAAAGGATATAGCTGGTCTCTCAGGATGGCATAAAGCCTTCAAGGAAAACTATCCACCAGATGAGTTTGTAGAGCTGTTGCAGAGTTTTGTACAATATGCTAGTGGCCTTCCTTTAGCTCATAAAGTACTAGGGTCATACTTGTCTGGAAGAAGTATAAATGAATGGTCAGAGGCATTGGATAGACTTGATGAAGATCCGGATAATGACATTTTTAGTGTGCTTCAAATAAGTTTTGATGGACTAAAGGAGTTAGATCAGAAAATCTTTTTAGACATTGCTTGTTTCTTCAATGGCGAGGATCATGCTCGTGTAAAAGGGATATTGAAAAGTTGTGGCTTTTGTCCTGAAATCGGTATAATTGACCTCATTAATAAATCTCTGATTAAAGTTGAAAGGAAAAAACTGTGGATGCATGATTTACTACAACAATTGGGTTGGCATATTGTTCGTCGAGAATCTCCTTTCCCGGGCAATCGTAGCAGGTTGTGGCTTAATGACAGTGCCAACAAGTACAAAGGCAGCAGGTCATGGCATGTTGAGGATACTCGTGATGCACTCACTGAAAATACACTTTCTCTGAAAATGCTAGTACTCATGGATCTTTCGGACTGCCAATATTTAACCAAGACCCTGGACTTCAGTAAGGTCCCAAAGCTTCAGAGATTGATCCTTAAAGGTTGTAAAGCGTTATCGGAGGTTCACCGAACAATTGGGGATCTCCATCATTTGGTTTTATTGAACTTGAAAGGTTGTGAAAGTCTAGAGAACCTTCCTCAATCCATCGGGTTGAGATCTCTTCGAACTTTTATTCTTTCGGGATGTTCAAAACTTGGACACTTTCCAGAGATTGTGGGGAACATGGATACTTTATCAGAACTTTGTTTAGATGGCACGGCTATATTGGAGCTACCTGTATCAATCCAGCATTTGGGAGGCGTTATTTTGCTAGATCTAAGAGGCTGCAAGAACCTTCTGAGTCTTCCCAGTGTCCTTTGCAGTAGTTTGACGTCACTGAAATTTCTCTATCTGTCATTGTGCTCAAGTATGGAAGAACTGCCAGAAAACATAGGCTGCTTGAAACACTTGGAGGAGCTTGATGTATGTGATACTGCTATAACAAAAGTGCCCAAGTCAATTTCAGGCCTCAAGAATCTTAAACTTTTGTGTTTCCATGGATGCTCTAGATCCAGAGGTTTAGAACTGCCAAACTTGTTCTCAGGTTTAAGATCTTTGACAACACTAAACCTAGGCGGATGCAACCTTGCAGCAATCCCGGGTGACATTGGCGAATTAAGCTCACTGCAGAGTTTGGATTTAAGTGAAAACAACTTTTCCACTATACCTCAGAGCATCTCTCATCTTTCTGAGCTTACAGAAATTTCTTTGTTTAAGTGTAGCAATCTACAGTTGTTGCCAAAAGAATTTCCATCGAGTTTAAGAAATGTCGACATACGTTATTGTCATATGCTGAAAAGTTCCTCAGATGGTTGGAGAAAATGGGCTTCTCAAAAGGGTTTGAGTATCATAAATTGTCGAAGACCAGAGGAGGATGAACATTTGTCAAAGCTACCTGAACTCCCCAAGTTCCCTTTGAAACAACTGCAACAACTCAATCTTTCCAACTGCCAACACTTGACCAAGATCCCAGAGTTGAATGAGATGCCATATATTCAGAAATTGATACTTGAAGGTTGTGAACAATTATCAGAGGTTCACCCAACAATTGGGAGCCTACAATTTCTGGTTTTATTGAATTTGAAAGGTTGCACAAGTCTAGAGAGCCTTCCTCAATCCATCAGCTTGAGATATCTTCGCACTTTTATTCTTTCGGGATGCTCAAAACTTAGAGAGTTTCCGGAGATTATGGTGAACATGGATAGTTTATCAGAACTTTATTTAGATGGCACGGCTCTACGGGAGCTGCCTGTATCAATCCAGCATTTGAAAGGCCTTACTTTGCTAAATCTAAGTGGCTGCAGGAACCTTGCAACTGTTCCAGTGCTTCCAAGTCTAAAATCTCTCAATCTGTCAGGTTGCTCCCGTATATCTAGATTGCCAACAGACCTGGGAAGAATGGAACATTTGGAGGAGCTTGATGCCTCTGAAACTGCTATAACTGAAGTACCCTATTCCATTTTATATTTGGAGAAGCTCAAAGTGTTATCTTTCTGTGGATGTTTTGGTTTGCAGTTACCTAACTGGTTCTCGAATTTACGATCTTTGAGATCATTAGATCTACGTAAATGTGGTCTACCAGAAAATGTTCATGATAGACTTTTTTGGTTGACTTCACTGCAGAGTTTGGATTTGGGTGAAAACATTTTTGTGAGTATACCTAATGAAATTGGTCTACTATCCTCTTTGCAGAGATTGGATTTCAGTGGAAACAATTTGGTGAGTGTATCAAATGAAATTGGTTCGTTGTCCTCTTTGCAGTGCTTGGATTTGAGTGAAAACAACTTTGTGAGTATACCTGAAAGCATCTCTCAACTTTCTGAGCTTACAGAACTTCGCTTGTTTAGGTGTAGTAAGCTACAGTCACTGCCAAGAAATCTTCCATTCAATCTAAAACATGTCAATGCACGAGAATGTCCCATGTTGAAGAATAATGCAGATACTTTGACAATATGGGCTTCTGGGAAGCGGTTCTGTTTCATAAATTGTGGACAATCAGACCAGGATAATGATCAGCCAAGCCACGTTCCAGTTCCAGTTCCGGAAGACCGTATTGGACTACTCTTTTCTAAATACATGCAGGATCGAATCTATGGTAAAAAACCATTTGAATTTCGTTTTCCACATAGTACAAGGATTCCCCAATTGTGGAGTCATTGGAGAAGTGGCCCTTCTGTGGCAGTCCCGCTATCTGATACTAACAGTTCATGGATTGGACTTGCTCTATTTGTTGTTTTCGAAATCCTTGAGAAAGAAAAATTTGATAACAGTTGGGAATCAGAGGAGACCATCTGTGAATTTCACACCAATCATGGGCGTGAAAGTTCACTAGTTTTTCAAAACTTCCTAGACTTCAGGACTGGTTCATATGGACTTTGTTGCTACGAACCACGAGGTGGGCAATTTGGTGGGTTGTTTGATGAACCACTTCTTCAAGCTTCAGTTTCAACAAAGAGACCAGATTTAAAAGTGAGAGGTTGTGGGTTGCATCTGATATCAGAGCATGATGCTGCAGAGTTTGTTCAAAATTTAATAAACCAGACTGCTATTCAACACCTGGACTTCAATTTTAATCGACATTGTGAGGAAATATTAGAAAAGGCAACGATTTGTGACCTGATAGAGCTAGACCCTGATGATCTATGGGAACTAGGATCTACTTCAACTGTGAATGAAGATGACTGCCGCATCAGTAGCTGTAAAATCCAACTCAGAGCACAGCTGTCAATCCTCTATGAGGGACGCAATGGATGTGAGAAGAGTTTCCATGTTTGTCTTCCTGATTCACCAAATATAATGTCAATTCCGACCTGGTTCATCAATCGCCAAGCAGGTGATGTAACACTATGTTACATTAGTGAAAACTTATTTGCTGATCAGAGATGGGTGGGATTAGAACTATATGTTGTATTTGCCCGGCGTACACCTGCTCCAAGTGATGGCCATATTAATAGTTTTTTCTTTCATGTTGAGTTGTGTGGTCATGATAATGGAAGTCTAATAATGCATGGATCCCTCAAGAGAGAGAGTTGTCTTGGAGAGTCAAATCAACTTGTTGTATTACATTTACCACGTGTTTATTTTCAAGAACAGTTGAATCAATGTCAGGTTATTAGCGCGTTGTTTACAACCAGTACCCCAGAATTGGAGGTTATAGTGTGTGGTAGCCGTTTAGTTTATCAGCAAGATTTGAAAGACTTGATCCATTCATTGACTGCGGCTGCAAGCACTTTTGGGGAACATGTCCTCACTCAACTTTGTTCCCAGGCCTGCACCCAAACTGATGATCAGATCATTGCAGTGAAAGGAGACATGGCCATCAATTGCGATTCCTCATTTCAAAACAGAAGAAGTACAGCACAATTACCAGAACAAGTTCTTACTTCACTAATGCGGCTGAAAAGTTACAGATGCTATCTTCGGCAAAAGCAAGTTTTTGTAGAGTCTGAACCTACCCCATTAGTTCACTCCGGATCAGTGATACCAGTCCATGCCAAAGGCTGTCTTTTGGAATACAGGGAAAGCAACTCAGAAAATGTAGCAGAAGAAGACACAAAGTCGTCATTGGCTTATTGTCATCATTTACGTATAATGGCTGAGACTCAACTTTACGGACGCTATAGTTCATTTCAGTTGAAAACATCCCTCCATTTGTTACTCCAACACTCCAAGGTGGCCACTCTCAGTCTTGGTGGACATACAATTTCTTCTCTCAAGAATTTTGATCCTTCCTCTGCATGCAATATTATTTGTTTCTCTGACAAGGAAATTCCAGTGTGGTTCAAACATGAGATGTCTTATCAACTGTCTTCTAGGTCCAGGGTGGGAATCAAACTACCGCCAAAATTGCAGGAGGATGGGAATTGGAGAGGACTTGCTATATGTGTTGCCTTTGAAGTTCATGACCAGCGTCCAACTACCTCCCCGGTCAAACTTCTCTGTCACTTGAGAGCCAAGGACAACTATTGCTTGAATCCTATCCCTATGTGTCCAATCACTGAAGAGAAACTCAAGTCGTTGCATGTTGATAGATTCATTTGGCTAAGCTACATCCCCCGTGTTTTGCTAACAGAGTTCAATGTGGTTAGTGATGTAGAGGCCAGAATTTATGTTAGTTGCAAAGGCTTGACAGTGGAGAAGTGTGGTATCCGTCTCTTGTACCGGCAAGAAGAGGGGGAGTTTGAGAGCGTGATAACTGAGTGCTGGACCTCTTTCTTTGATAATCTGTCTTTCATCCGTAGACTTGTAGAAGCAGATGATCAAAATGTTCCACCACGGACAAGGCATGAACTTCCAATGCTTGAAGGGCATATCAAGGTCTTTGATCCGGATATACTATATAATGCAATTCCCACTAGTACTGAAATTCCAAAGTGGTTCGGGAATCCCATTGAGGCCAAGTATGACGATTACGCCAAAGCCCACACGTGGGAATTCCAGTTACCACCATCTTTGAGTGACAAAAACTGGATAGGATTGGCTATCTGTGCATCACATCGTATTAGCAGAGCGTATTTGGTGGAACGTGATTCCTACCCCTTTATTTTAAGATTGAAAACTGGAAACAATGGCTTGTCATCTCTCCATCGGTATCAGATGAACAATGAAGAATCTGAGTTCCTAAAGCGTTGTTGTAATGTGCATGGTGAATTCATTTGGCTCTCCTACATACCACGACGCTGGTTTCTACATCAGCTAAATGACGAGTCTGTCCTCTTTGTTTTATCTGGTGAGTACTGCTGGAAGCCGCGCACGTTATATCTCCGTTTTGTGTATGCGCATGAGGGGGAAGAGTTTAAGCAGCTCTGTTTCAATCTTCATCGACCCCCTGCCTGCCCAACAGTAAAAAGATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04064 ko04115 ko04210 map04064 map04115 map04210
Pfam Domains
Protein Families
Death LRR_8 Peptidase_S68 ZU5

Protein Analysis

2146

Amino Acids

242.53

Weight (kDa)

6.07

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 168 - 313 1.6e-50 TIR domain
TIR_2 PF13676 170 - 266 8.5e-16 TIR domain
WHD_ROQ1 PF23282 385 - 454 2.9e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 476 - 597 1.7e-06 Leucine-rich repeat region
LRR_13 PF23286 565 - 632 9.5e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 616 - 717 2.8e-13 Leucine-rich repeat region
LRR_14 PF23598 709 - 768 2.9e-06 Leucine-rich repeat region
LRR_8 PF13855 710 - 764 1.5e-08 Leucine rich repeat
LRR_13 PF23286 908 - 983 1.8e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 946 - 1071 1.6e-13 Leucine-rich repeat region
LRR_13 PF23286 969 - 1186 3e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1038 - 1145 8.2e-12 Leucine-rich repeat region
LRR_8 PF13855 1065 - 1122 3e-06 Leucine rich repeat
LRR_8 PF13855 1088 - 1143 1.7e-06 Leucine rich repeat
C-JID PF20160 1779 - 1916 2.3e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
AarI CACCTGC 2 cut(s) 4400, 4513
AasI GACNNNNNNGTC 1 cut(s) 6287
AatII GACGTC 1 cut(s) 1913
Acc16I TGCGCA 2 cut(s) 106, 6357
Acc36I ACCTGC 4 cut(s) 1374, 1416, 4400, 4513
AccB1I GGYRCC 1 cut(s) 132
AccB7I CCANNNNNTGG 5 cut(s) 1630, 1831, 2627, 3974, 4517
AccI GTMKAC 6 cut(s) 2349, 3154, 3235, 3253, 3373, 5818
AccII CGCG 2 cut(s) 4697, 6328
AccIII TCCGGA 5 cut(s) 1099, 2758, 3619, 5048, 5905
AcoI YGGCCR 4 cut(s) 307, 4519, 4893, 5253
AcuI CTGAAG 7 cut(s) 599, 1525, 1544, 3042, 3916, 4002, 5598
AcvI CACGTG 2 cut(s) 4652, 6006
AcyI GRCGYC 1 cut(s) 1910
AdeI CACNNNGTG 1 cut(s) 3974
AflIII ACRYGT 4 cut(s) 3475, 4651, 4819, 6003
AhdI GACNNNNNGTC 1 cut(s) 5134
AhlI ACTAGT 2 cut(s) 3907, 5933
AjnI CCWGG 9 cut(s) 162, 420, 1533, 2967, 3579, 4134, 4388, 4842, 5396
AjuI GAANNNNNNNTTGG 4 cut(s) 229, 261, 3615, 3647
AloI GAACNNNNNNTCC 2 cut(s) 753, 785
Alw21I GWGCWC 3 cut(s) 159, 1943, 4297
AlwNI CAGNNNCTG 2 cut(s) 3445, 4275
Ama87I CYCGRG 2 cut(s) 1369, 2176
Aor13HI TCCGGA 5 cut(s) 1099, 2758, 3619, 5048, 5905
ArsI GACNNNNNNTTYG 2 cut(s) 4361, 4393
AseI ATTAAT 2 cut(s) 1272, 4527
Asp700I GAANNNNTTC 7 cut(s) 612, 771, 3165, 3548, 4005, 4340, 4953
AspA2I CCTAGG 1 cut(s) 2149
AspLEI GCGC 4 cut(s) 107, 4697, 6330, 6358
AspS9I GGNCC 4 cut(s) 1549, 3746, 5394, 5784
AsuC2I CCSGG 6 cut(s) 1370, 1371, 2177, 2178, 4495, 5504
AsuII TTCGAA 3 cut(s) 339, 1702, 3816
AvaI CYCGRG 2 cut(s) 1369, 2176
AvaII GGWCC 3 cut(s) 1549, 5394, 5784
AvrII CCTAGG 1 cut(s) 2149
BaeGI GKGCMC 1 cut(s) 2031
BaeI ACNNNNGTAYC 2 cut(s) 3009, 3042
BalI TGGCCA 4 cut(s) 309, 4521, 4895, 5255
BamHI GGATCC 1 cut(s) 4589
BanI GGYRCC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 4 cut(s) 1209, 1449, 3486, 3969
BbrPI CACGTG 2 cut(s) 4652, 6006
BbsI GAAGAC 7 cut(s) 502, 1874, 2446, 3630, 4346, 5133, 5379
Bbv12I GWGCWC 3 cut(s) 159, 1943, 4297
BceAI ACGGC 4 cut(s) 97, 1812, 2826, 4731
BcgI CGANNNNNNTGC 4 cut(s) 1187, 1221, 1331, 1365
BciT130I CCWGG 9 cut(s) 164, 422, 1535, 2969, 3581, 4136, 4390, 4844, 5398
BciVI GTATCC 3 cut(s) 1438, 1759, 5735
BclI TGATCA 5 cut(s) 346, 3589, 4453, 4864, 5836
BcnI CCSGG 6 cut(s) 1370, 1371, 2177, 2178, 4495, 5504
BcuI ACTAGT 2 cut(s) 3907, 5933
BfmI CTRYAG 7 cut(s) 2204, 2306, 2878, 3197, 3437, 4089, 5197
BfuAI ACCTGC 4 cut(s) 1374, 1416, 4400, 4513
BfuI GTATCC 3 cut(s) 1438, 1759, 5735
BglII AGATCT 5 cut(s) 401, 1694, 1852, 2129, 3136
BlnI CCTAGG 1 cut(s) 2149
BlpI GCTNAGC 2 cut(s) 5, 5621
BmcAI AGTACT 4 cut(s) 1029, 1494, 5938, 6314
Bme18I GGWCC 3 cut(s) 1549, 5394, 5784
BmeRI GACNNNNNGTC 1 cut(s) 5134
BmeT110I CYCGRG 2 cut(s) 1369, 2176
BmgT120I GGNCC 4 cut(s) 1549, 3746, 5394, 5784
BmiI GGNNCC 5 cut(s) 134, 1551, 2633, 2885, 4591
BmrI ACTGGG 2 cut(s) 505, 1881
BmuI ACTGGG 2 cut(s) 505, 1881
BoxI GACNNNNGTC 1 cut(s) 5487
BpiI GAAGAC 7 cut(s) 502, 1874, 2446, 3630, 4346, 5133, 5379
BplI GAGNNNNNCTC 4 cut(s) 2242, 2274, 2674, 2706
BpmI CTGGAG 1 cut(s) 198
Bpu1102I GCTNAGC 2 cut(s) 5, 5621
Bpu14I TTCGAA 3 cut(s) 339, 1702, 3816
BpuEI CTTGAG 8 cut(s) 1927, 2033, 2725, 3845, 4580, 5276, 5545, 5573
BpuMI CCSGG 6 cut(s) 1370, 1371, 2177, 2178, 4495, 5504
BsaAI YACGTR 4 cut(s) 3143, 4652, 5165, 6006
BsaBI GATNNNNATC 3 cut(s) 1095, 1623, 3588
BsaHI GRCGYC 1 cut(s) 1910
BsaI GGTCTC 3 cut(s) 923, 3857, 4027
BsaWI WCCGGW 6 cut(s) 259, 1099, 2758, 3619, 5048, 5905
BsaXI ACNNNNNCTCC 4 cut(s) 2463, 2493, 4492, 4522
Bse118I RCCGGY 1 cut(s) 5739
Bse3DI GCAATG 3 cut(s) 1176, 4330, 4872
Bse8I GATNNNNATC 3 cut(s) 1095, 1623, 3588
BseAI TCCGGA 5 cut(s) 1099, 2758, 3619, 5048, 5905
BseBI CCWGG 9 cut(s) 164, 422, 1535, 2969, 3581, 4136, 4390, 4844, 5398
BseJI GATNNNNATC 3 cut(s) 1095, 1623, 3588
BseMI GCAATG 3 cut(s) 1176, 4330, 4872
BseRI GAGGAG 4 cut(s) 2006, 2462, 3005, 3875
BseSI GKGCMC 1 cut(s) 2031
BsgI GTGCAG 1 cut(s) 4834
Bsh1236I CGCG 2 cut(s) 4697, 6328
BshNI GGYRCC 1 cut(s) 132
BsiHKAI GWGCWC 3 cut(s) 159, 1943, 4297
BsiHKCI CYCGRG 2 cut(s) 1369, 2176
BslFI GGGAC 5 cut(s) 774, 1535, 3480, 3746, 4332
BsmBI CGTCTC 2 cut(s) 131, 5735
BsmFI GGGAC 5 cut(s) 774, 1535, 3480, 3746, 4332
BsmI GAATGC 1 cut(s) 749
Bso31I GGTCTC 3 cut(s) 923, 3857, 4027
BsoBI CYCGRG 2 cut(s) 1369, 2176
Bsp119I TTCGAA 3 cut(s) 339, 1702, 3816
Bsp1286I GDGCHC 4 cut(s) 159, 1943, 2031, 4297
Bsp13I TCCGGA 5 cut(s) 1099, 2758, 3619, 5048, 5905
Bsp1407I TGTACA 1 cut(s) 991
Bsp1720I GCTNAGC 2 cut(s) 5, 5621
Bsp19I CCATGG 1 cut(s) 2074
BspEI TCCGGA 5 cut(s) 1099, 2758, 3619, 5048, 5905
BspFNI CGCG 2 cut(s) 4697, 6328
BspHI TCATGA 3 cut(s) 3169, 4561, 5479
BspLI GGNNCC 5 cut(s) 134, 1551, 2633, 2885, 4591
BspMAI CTGCAG 4 cut(s) 2208, 2882, 3201, 4093
BspMI ACCTGC 4 cut(s) 1374, 1416, 4400, 4513
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 3 cut(s) 339, 1702, 3816
BspT107I GGYRCC 1 cut(s) 132
BspTNI GGTCTC 3 cut(s) 923, 3857, 4027
BsrDI GCAATG 3 cut(s) 1176, 4330, 4872
BsrFI RCCGGY 1 cut(s) 5739
BsrGI TGTACA 1 cut(s) 991
BssAI RCCGGY 1 cut(s) 5739
BssNAI GTATAC 2 cut(s) 3236, 3374
BssNI GRCGYC 1 cut(s) 1910
BssSI CACGAG 4 cut(s) 1209, 1449, 3486, 3969
BssT1I CCWWGG 4 cut(s) 2074, 2149, 5247, 5532
Bst1107I GTATAC 2 cut(s) 3236, 3374
Bst2BI CACGAG 4 cut(s) 1209, 1449, 3486, 3969
Bst2UI CCWGG 9 cut(s) 164, 422, 1535, 2969, 3581, 4136, 4390, 4844, 5398
Bst6I CTCTTC 7 cut(s) 164, 654, 1704, 4331, 5574, 5742, 6363
BstACI GRCGYC 1 cut(s) 1910
BstAUI TGTACA 1 cut(s) 991
BstBAI YACGTR 4 cut(s) 3143, 4652, 5165, 6006
BstBI TTCGAA 3 cut(s) 339, 1702, 3816
BstC8I GCNNGC 4 cut(s) 4804, 4849, 5317, 6412
BstDSI CCRYGG 2 cut(s) 2074, 5852
BstENI CCTNNNNNAGG 1 cut(s) 4313
BstFNI CGCG 2 cut(s) 4697, 6328
BstHHI GCGC 4 cut(s) 107, 4697, 6330, 6358
BstMWI GCNNNNNNNGC 7 cut(s) 130, 1380, 2196, 4272, 5425, 5884, 6065
BstNI CCWGG 9 cut(s) 164, 422, 1535, 2969, 3581, 4136, 4390, 4844, 5398
BstNSI RCATGY 6 cut(s) 1439, 3479, 3658, 4823, 5319, 5603
BstPAI GACNNNNGTC 1 cut(s) 5487
BstSFI CTRYAG 7 cut(s) 2204, 2306, 2878, 3197, 3437, 4089, 5197
BstSLI GKGCMC 1 cut(s) 2031
BstSNI TACGTA 2 cut(s) 3143, 5165
BstUI CGCG 2 cut(s) 4697, 6328
BstV2I GAAGAC 7 cut(s) 502, 1874, 2446, 3630, 4346, 5133, 5379
BstXI CCANNNNNNTGG 2 cut(s) 1534, 6258
BstZ17I GTATAC 2 cut(s) 3236, 3374
BsuI GTATCC 3 cut(s) 1438, 1759, 5735
BtgI CCRYGG 2 cut(s) 2074, 5852
BtgZI GCGATG 1 cut(s) 93
BtsI GCAGTG 5 cut(s) 2201, 3194, 3344, 3443, 4884
BveI ACCTGC 4 cut(s) 1374, 1416, 4400, 4513
Cac8I GCNNGC 4 cut(s) 4804, 4849, 5317, 6412
CaiI CAGNNNCTG 2 cut(s) 3445, 4275
CciI TCATGA 3 cut(s) 3169, 4561, 5479
CfoI GCGC 4 cut(s) 107, 4697, 6330, 6358
Cfr10I RCCGGY 1 cut(s) 5739
Cfr13I GGNCC 4 cut(s) 1549, 3746, 5394, 5784
Cfr9I CCCGGG 2 cut(s) 1369, 2176
CseI GACGC 5 cut(s) 113, 4329, 5202, 5477, 6264
CsiI ACCWGGT 1 cut(s) 4388
CspCI CAANNNNNGTGG 2 cut(s) 6012, 6047
DraI TTTAAA 2 cut(s) 903, 4044
DraIII CACNNNGTG 1 cut(s) 3974
DrdI GACNNNNNNGTC 1 cut(s) 6287
DriI GACNNNNNGTC 1 cut(s) 5134
DseDI GACNNNNNNGTC 1 cut(s) 6287
EaeI YGGCCR 4 cut(s) 307, 4519, 4893, 5253
Eam1104I CTCTTC 7 cut(s) 164, 654, 1704, 4331, 5574, 5742, 6363
Eam1105I GACNNNNNGTC 1 cut(s) 5134
EarI CTCTTC 7 cut(s) 164, 654, 1704, 4331, 5574, 5742, 6363
EciI GGCGGA 1 cut(s) 2169
Ecl136II GAGCTC 1 cut(s) 157
Eco105I TACGTA 2 cut(s) 3143, 5165
Eco130I CCWWGG 4 cut(s) 2074, 2149, 5247, 5532
Eco147I AGGCCT 3 cut(s) 2046, 2853, 4847
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 3 cut(s) 923, 3857, 4027
Eco32I GATATC 2 cut(s) 2711, 4074
Eco47I GGWCC 3 cut(s) 1549, 5394, 5784
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 7 cut(s) 599, 1525, 1544, 3042, 3916, 4002, 5598
Eco72I CACGTG 2 cut(s) 4652, 6006
Eco88I CYCGRG 2 cut(s) 1369, 2176
EcoICRI GAGCTC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 4313
EcoO109I RGGNCCY 1 cut(s) 1549
EcoRI GAATTC 2 cut(s) 6011, 6227
EcoRII CCWGG 9 cut(s) 162, 420, 1533, 2967, 3579, 4134, 4388, 4842, 5396
EcoRV GATATC 2 cut(s) 2711, 4074
EcoT14I CCWWGG 4 cut(s) 2074, 2149, 5247, 5532
EcoT22I ATGCAT 2 cut(s) 1317, 4587
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 4 cut(s) 2074, 2149, 5247, 5532
Esp3I CGTCTC 2 cut(s) 131, 5735
FalI AAGNNNNNCTT 4 cut(s) 3733, 3765, 5067, 5099
FaqI GGGAC 5 cut(s) 774, 1535, 3480, 3746, 4332
FauI CCCGC 2 cut(s) 545, 3771
FauNDI CATATG 2 cut(s) 2367, 3946
FbaI TGATCA 5 cut(s) 346, 3589, 4453, 4864, 5836
FblI GTMKAC 6 cut(s) 2349, 3154, 3235, 3253, 3373, 5818
FriOI GRGCYC 1 cut(s) 159
FspAI RTGCGCAY 2 cut(s) 106, 6357
FspI TGCGCA 2 cut(s) 106, 6357
GlaI GCGC 4 cut(s) 106, 4696, 6329, 6357
GsuI CTGGAG 1 cut(s) 198
HgaI GACGC 5 cut(s) 113, 4329, 5202, 5477, 6264
HhaI GCGC 4 cut(s) 107, 4697, 6330, 6358
Hin1I GRCGYC 1 cut(s) 1910
Hin6I GCGC 4 cut(s) 105, 4695, 6328, 6356
HinP1I GCGC 4 cut(s) 105, 4695, 6328, 6356
HincII GTYRAC 2 cut(s) 2350, 3189
HindII GTYRAC 2 cut(s) 2350, 3189
HindIII AAGCTT 4 cut(s) 611, 897, 1556, 4014
Hpy99I CGWCG 3 cut(s) 154, 1356, 6258
HpyF10VI GCNNNNNNNGC 7 cut(s) 130, 1380, 2196, 4272, 5425, 5884, 6065
Hsp92I GRCGYC 1 cut(s) 1910
HspAI GCGC 4 cut(s) 105, 4695, 6328, 6356
Kpn2I TCCGGA 5 cut(s) 1099, 2758, 3619, 5048, 5905
Ksp22I TGATCA 5 cut(s) 346, 3589, 4453, 4864, 5836
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 8 cut(s) 154, 1807, 1993, 2631, 2821, 2945, 2992, 4513
MabI ACCWGGT 1 cut(s) 4388
MfeI CAATTG 5 cut(s) 1331, 1611, 2625, 3722, 4900
MhlI GDGCHC 4 cut(s) 159, 1943, 2031, 4297
MlsI TGGCCA 4 cut(s) 309, 4521, 4895, 5255
MluNI TGGCCA 4 cut(s) 309, 4521, 4895, 5255
MlyI GAGTC 8 cut(s) 218, 671, 1888, 3739, 4628, 5030, 5173, 6293
MmeI TCCRAC 7 cut(s) 132, 2372, 2547, 4309, 4409, 5263, 5516
Mox20I TGGCCA 4 cut(s) 309, 4521, 4895, 5255
Mph1103I ATGCAT 2 cut(s) 1317, 4587
MroI TCCGGA 5 cut(s) 1099, 2758, 3619, 5048, 5905
MroXI GAANNNNTTC 7 cut(s) 612, 771, 3165, 3548, 4005, 4340, 4953
MscI TGGCCA 4 cut(s) 309, 4521, 4895, 5255
MslI CAYNNNNRTG 7 cut(s) 1209, 3227, 3480, 3490, 3894, 4566, 4877
Msp20I TGGCCA 4 cut(s) 309, 4521, 4895, 5255
MspA1I CMGCKG 2 cut(s) 218, 4300
MunI CAATTG 5 cut(s) 1331, 1611, 2625, 3722, 4900
Mva1269I GAATGC 1 cut(s) 749
MvaI CCWGG 9 cut(s) 164, 422, 1535, 2969, 3581, 4136, 4390, 4844, 5398
MvnI CGCG 2 cut(s) 4697, 6328
MwoI GCNNNNNNNGC 7 cut(s) 130, 1380, 2196, 4272, 5425, 5884, 6065
NciI CCSGG 6 cut(s) 1370, 1371, 2177, 2178, 4495, 5504
NcoI CCATGG 1 cut(s) 2074
NdeI CATATG 2 cut(s) 2367, 3946
NlaIV GGNNCC 5 cut(s) 134, 1551, 2633, 2885, 4591
NmuCI GTSAC 7 cut(s) 436, 1911, 2180, 3441, 4190, 5519, 6035
NsbI TGCGCA 2 cut(s) 106, 6357
NsiI ATGCAT 2 cut(s) 1317, 4587
NspI RCATGY 6 cut(s) 1439, 3479, 3658, 4823, 5319, 5603
NspV TTCGAA 3 cut(s) 339, 1702, 3816
PaeI GCATGC 1 cut(s) 5319
PagI TCATGA 3 cut(s) 3169, 4561, 5479
PaqCI CACCTGC 2 cut(s) 4400, 4513
PceI AGGCCT 3 cut(s) 2046, 2853, 4847
PciI ACATGT 2 cut(s) 3475, 4819
PciSI GCTCTTC 1 cut(s) 164
PctI GAATGC 1 cut(s) 749
PdmI GAANNNNTTC 7 cut(s) 612, 771, 3165, 3548, 4005, 4340, 4953
PflFI GACNNNGTC 1 cut(s) 195
PflMI CCANNNNNTGG 5 cut(s) 1630, 1831, 2627, 3974, 4517
PleI GAGTC 8 cut(s) 217, 670, 1887, 3738, 4627, 5029, 5173, 6292
PmaCI CACGTG 2 cut(s) 4652, 6006
PmlI CACGTG 2 cut(s) 4652, 6006
PpsI GAGTC 8 cut(s) 217, 670, 1887, 3738, 4627, 5029, 5173, 6292
Ppu21I YACGTR 4 cut(s) 3143, 4652, 5165, 6006
PpuMI RGGWCCY 1 cut(s) 1549
PscI ACATGT 2 cut(s) 3475, 4819
PshAI GACNNNNGTC 1 cut(s) 5487
PshBI ATTAAT 2 cut(s) 1272, 4527
PsiI TTATAA 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 1549
Psp6I CCWGG 9 cut(s) 162, 420, 1533, 2967, 3579, 4134, 4388, 4842, 5396
PspCI CACGTG 2 cut(s) 4652, 6006
PspGI CCWGG 9 cut(s) 162, 420, 1533, 2967, 3579, 4134, 4388, 4842, 5396
PspN4I GGNNCC 5 cut(s) 134, 1551, 2633, 2885, 4591
PspPI GGNCC 4 cut(s) 1549, 3746, 5394, 5784
PspPPI RGGWCCY 1 cut(s) 1549
PstI CTGCAG 4 cut(s) 2208, 2882, 3201, 4093
PstNI CAGNNNCTG 2 cut(s) 3445, 4275
PsyI GACNNNGTC 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 4300
RseI CAYNNNNRTG 7 cut(s) 1209, 3227, 3480, 3490, 3894, 4566, 4877
SacI GAGCTC 1 cut(s) 159
SalI GTCGAC 1 cut(s) 2348
SapI GCTCTTC 1 cut(s) 164
Sau96I GGNCC 4 cut(s) 1549, 3746, 5394, 5784
ScaI AGTACT 4 cut(s) 1029, 1494, 5938, 6314
SchI GAGTC 8 cut(s) 218, 671, 1888, 3739, 4628, 5030, 5173, 6293
SduI GDGCHC 4 cut(s) 159, 1943, 2031, 4297
SexAI ACCWGGT 1 cut(s) 4388
SfcI CTRYAG 7 cut(s) 2204, 2306, 2878, 3197, 3437, 4089, 5197
SfuI TTCGAA 3 cut(s) 339, 1702, 3816
SinI GGWCC 3 cut(s) 1549, 5394, 5784
SmaI CCCGGG 2 cut(s) 1371, 2178
SmiMI CAYNNNNRTG 7 cut(s) 1209, 3227, 3480, 3490, 3894, 4566, 4877
SmlI CTYRAG 8 cut(s) 1942, 2048, 2704, 3824, 4595, 5291, 5524, 5588
SmoI CTYRAG 8 cut(s) 1942, 2048, 2704, 3824, 4595, 5291, 5524, 5588
SnaBI TACGTA 2 cut(s) 3143, 5165
SpeI ACTAGT 2 cut(s) 3907, 5933
SphI GCATGC 1 cut(s) 5319
SseBI AGGCCT 3 cut(s) 2046, 2853, 4847
SspI AATATT 4 cut(s) 825, 1520, 4170, 5323
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 3 cut(s) 2046, 2853, 4847
StyI CCWWGG 4 cut(s) 2074, 2149, 5247, 5532
TatI WGTACW 8 cut(s) 991, 1027, 1413, 1492, 3707, 4931, 5936, 6312
TauI GCSGC 4 cut(s) 4266, 4799, 4975, 6328
TseFI GTSAC 7 cut(s) 436, 1911, 2180, 3441, 4190, 5519, 6035
Tsp45I GTSAC 7 cut(s) 436, 1911, 2180, 3441, 4190, 5519, 6035
TspGWI ACGGA 5 cut(s) 5205, 5717, 5804, 5869, 6332
TspMI CCCGGG 2 cut(s) 1369, 2176
Tth111I GACNNNGTC 1 cut(s) 195
Van91I CCANNNNNTGG 5 cut(s) 1630, 1831, 2627, 3974, 4517
VpaK11BI GGWCC 3 cut(s) 1549, 5394, 5784
VspI ATTAAT 2 cut(s) 1272, 4527
XagI CCTNNNNNAGG 1 cut(s) 4313
XbaI TCTAGA 5 cut(s) 398, 1663, 2084, 2677, 2951
XceI RCATGY 6 cut(s) 1439, 3479, 3658, 4823, 5319, 5603
XcmI CCANNNNNNNNNTGG 2 cut(s) 418, 3043
XmaI CCCGGG 2 cut(s) 1369, 2176
XmaJI CCTAGG 1 cut(s) 2149
XmiI GTMKAC 6 cut(s) 2349, 3154, 3235, 3253, 3373, 5818
XmnI GAANNNNTTC 7 cut(s) 612, 771, 3165, 3548, 4005, 4340, 4953
ZraI GACGTC 1 cut(s) 1911
ZrmI AGTACT 4 cut(s) 1029, 1494, 5938, 6314
Zsp2I ATGCAT 2 cut(s) 1317, 4587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.