Rw5G026490

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000307
Physical Location & Seq
Forward (+)
38349355 .. 38357116
7762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026490.1

Sequence Viewer

Length: 4215 bp
ATGCGCTACCTCAGATTGTTGAAGATTTGTAATGTCAAATTTCTGGGTGCTCCTTTTATACATCTCTCTAAACACTTAAGAGTTTTGGAGTGGCATGAATGTCCTTTAGAATCTCTGCCATCAAGTTGTCAACCGGATCGGCTTGTTGAACTTAAGATGCCTAATAGCCGCATTGAACAACTATGGAATGAAAGGCTTGCATTAAAAATGCTAGTGCTAATTGATCTTTCCTACTGCCAATACTTAAGGAGGACTCTCGACTTCAGTAGGGTCCCAAATCTTGAGCGGCTAAACTTTGAAGGTTGTAAAAGATTATCAGAAGTTCACCCAACAATTGGGGATCTAAAACATCTAGTCTTATTGAATATGAAAAATTGTGAAAGTCTAGAAAGCCTTGTCAACTCCATCAGCTTGGCATCTCTTGAAATGTTTATTCTTTCCGGATGTTCAAAGCTCAAAGAGTTTCCAGAGATTGTTGGAGGAATGAAATCTTTGGCAGAACTTTACTTGGATGGGACGGCTATAAGGAAGCTGCCGATATCAATCCAACACTTGAGTGGCCTTATTTTGCTGAATTTAAAAGGCTGCAAATACCTTCTGGGTCTTCCAAGCGTCGTTTGTAATCTGATATCTCTGAAGTATCTATATCTCTCAGGCTGCTCTCGTATTGACCAACTGCCAGAACATCTAGGGAGTTTGGAACATTTGCAGGTGCTTGATGCCTGTAAAACTGCTATAAGAAAAGTTCCATCATCCATCTTACTTCTGAAGAACCCCAAACAGTTGTGTTTTCCTGGAAGTAATGATCTGCAGATGGAATTCTCTGAATCCAGTGGTTTCCGCTACTCATCCTCACATTCATGTTCATTGATGGCATTAGATACCCGCTATGATCTTTTCTGCTGTGTCTCACAACCTGTTTCAGATTTGAATGAAAACAAAATGTTGATCAGCAAACCTGAAATCATCTCCGATGGATATTTGCAGGAGCTTGATACCTGGAGCACTCTGAATTGGAGTTGCAGTTTCCGTGAAACAGATTTAAGAAATAATTATCATTTGATGCTTTGTGACAATTCTCCTCAGATGTTCTTTGATTCCTGTCAATATTCATCTTTGACATCTTTACTTCTGAGTGGATGCAAGCTAGGCGAAGGAGCAATCCCTGATGATATTGGCTGCTTATACTCACTCCATTTGTTAGACTTGAGTGAAAACAATTTCGTGAGCATACCTGAAACCATTTCTCAACTTTCCAAGCTCAGAAAGCTTCTGTTGTTTAGGTGCAGCAAACTTCAGTATTTGCCAAAACAACTTCCACTGAGTTTAAAACGTGTAATTGCACGCCATTGTCCTATGCTGAGAACTACTTATCCAAATACTATCGTAAAATGGACTTCAGATGAAGGTTTCAGTTTCATAGATTGCAGATATTCAGACGAAAATGCAGACGATGATGTAGGATATATGTCCTTCCATAAGATCATTGAGGATCGAATTCATCATGACAAGCCATTTGAAATTCGTTTTTGTCATGGTCAACTTGATGGTGTTTCTCATGGTGATCGAATTCCGGACTGGTGCACTCAGTGGAACACTGGTTCTTCTGTTACATTGCAACTAACTGGTCCAGAAGACAAGACTAATAGTACGTGGATGGGATTAGCCTTATATGTTACTTTTGAATACTATCTGAATTATCAAGATCAAACAGAAATTATCTGTCATCTGAGCATCGACAAAGGTGGTCCTCAAATTCCTTTAGTTTTTCAAGACTTGGATGTTAGGTCACATGGACTCTGTTGCTACTTGCCACGAGGCTGCGGGGGGTTTGCAGGACAGCTGGATAAAGCCAGTCGAATTAATGCTACATTTTTGCTCACTACTTTACGGGTGAATTCGTGTGGGATACATCCAATATCCGAGCATGACGCTCCAATGTTTGCTCAGGATTTAGCCCGGACTGCTTTTGCTGACGCACGTGCTTTTGCTAAACAGGAAAAATTGTTTAATGACGCTGATGGGAAGGCACGTGAAGTTTGTCGACATGGTAAGAGAATGTTAGATAAGGCAACGAAATTGGAAAGATCGGGAGACGTGCTGGTACCTGCATACAATTCTTTAAATGTGAATCGACACAAGTGGAAAATTGATCCTGATATCCATCCCAGATTAGAGGAAGAGCTAAAGCTGTTCAGATTGCTTTCAGGGCTCTACGAGGGAAGCAATGGACGTCAGAAGAAATTTCATTTTTGTTTTCCAGATCAAGTAATTTCGCCCTGGTTCATCAATCACTACGCAGGGAATGTAGCACTTTGTGACCTTCCTCAGAACTTAATCGATGATAAGAGATGGATTGGATTAGAAGTGTATGTTGTGTTTACACCTGGTTACAGTGACCACAATCTTTCTGATTCCTTCCTTCATGTTGATTTAATGGCACATGAAAGTAGTGTAATGCATTCGTCCATCAAAATTGAGAGTTGTTCTGGGTCATGTCAACTTATTGTACTACATGTACCACGGATGCATTTTCCGGAACAGTTGACTCATTATCAGGTTATTAGCACGTTGTTTACAACCAGTACCCCAATTGTTGAGATTATAGTGTGCGGGAGCCGTTTGGTATATGAGCATGATCTGGAAGATTTAATCCTTTCATTAACTGCGTGTACATTGAGGAAGCCATATTCAATTGGCCAAGTTTATTCCCAGGCCCAAACATATGTTGAAACTACGGAAAAGAAAGCAGATGAAAATATCCAGTGCAATGCAAAGCGAGGAGAAATGTCATCCACTGATGGCCATTCATCGTTTCAAAGAAGTGTCAAACAAATTAAAGTACCAGATAAAGTTCGCAGTTCAATATTGCAGAAATATAGATGCCATATCCAAGGAAAGCAAGGTATACTAGAGTCTGACAAAATAATTGTCCACTCTAAATCCCTTCTTCTGGACTATAGCATCAAAGAAAATTTTACTGGAAAAGAATCAATAATGTCGTCGTTGGCATGTCATCATTTGCATATAGTGGCTGAGACTCACTTCTGGGGAAGCTATAGTGCCCGACAGTGGAAGAGATGCCTAGAGTTGTTGCTGAAATATTCTGAGGTGGCAACTCTCAGTCTTAATGGACGTGTAATCTCTGTAATGAAGCATTTTAGTCCCTCCTCGCCATTCAATTTTTGTTTCCCTAAAGATGAAGTGCTGCTAGACTGGATGAGAGATCGTCGACTCTGTGATGATAAAAGTTTGAGGGGACTTGTTATATGTGCAACTTTTTCATTTGACGATGATTCTGCCATGAACAATCTGGCTTCAGAAATTACTGTTGAACTCTTGTGTCACTTGAGTTACAATGGCTCCAGGTTTTGCGTGAACGCTGCCCCCATGCTTACGATCACTAAGGAGAAATTCCAGTGGCTTTGTTTCCGTGATTTCATTTGGGTTACGTATGTACCCCTTGTTATGCTAGCGGAGGTCAAAGTAATCAGTGATATAGAGGCTGATATATGTAGTAATTGCCCAGGCTTGAGGGTGGACTGCGCTTATACATGTGTCTTGTACCGGCAAAAGGAGGAGGAATTTAAGCAAAAAATAACTCAGTGCTGGACCTCCTTCTTTGATAATATCGATCTCCTCTGTCGGTTTGTACAAGCAGATGCTGAAAAAGGTGTAGAGACGAAGCACGAACTTCCAATGCTCGGCGGAAGTATGAAGGACCTTGATAGCAACTTGCTGTATGACGCAACACACCCATTTACTGAAATTCCGGATTGGTTTGGACATCCAAATGAAAGGTCTGGAGAGATCCAGTTACCACCATCTCTTGGCAGGGACGAAAATTGGATAGGAGTGGCTATATGTGCATCATATTCTCCCTTACTATTGAATCCTCAAGTTTCTGCGTACTTAAAATGTGTTTTGCGAACTGAGAAGAATAACGTGAAATCTGCCCACCGCTATCAGATGACCAAAAAAGAAGTCAAGTGCGTATCTTCTGAGAGAAAATTCATTTGGCTATCCTATCTTCCACGACACTGGTTTTTCTACCAGTTAAATGATGAGTCTGCTCTCACGGCTTCGTTTTCAAGTTCACCTGGTTTGTGGTTTAACGAGTGCGGTCTGCGTCTTGTATACCGGCAAGATGAGGAAGAGTTTAAGCAACTCTGTATCTCCTTAAACTCGTTGGCCGACAATCAAAAACCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1404

Amino Acids

160.3

Weight (kDa)

6.41

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 138 - 223 1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 204 - 264 6.1e-11 Leucine-rich repeat region
C-JID PF20160 1253 - 1385 2e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 700
AatII GACGTC 1 cut(s) 2236
Acc36I ACCTGC 2 cut(s) 700, 2116
Acc65I GGTACC 1 cut(s) 2104
AccB1I GGYRCC 1 cut(s) 2104
AccB7I CCANNNNNTGG 2 cut(s) 335, 3830
AccBSI CCGCTC 1 cut(s) 286
AccI GTMKAC 4 cut(s) 2044, 2908, 3232, 4137
AccIII TCCGGA 4 cut(s) 440, 1573, 2536, 3772
AclWI GGATC 5 cut(s) 144, 348, 1500, 2147, 3805
AcoI YGGCCR 3 cut(s) 2698, 2803, 4191
AcuI CTGAAG 6 cut(s) 247, 656, 788, 1280, 1383, 3303
AcvI CACGTG 2 cut(s) 1982, 2033
AcyI GRCGYC 1 cut(s) 2233
AdeI CACNNNGTG 2 cut(s) 1590, 2318
AfiI CCNNNNNNNGG 6 cut(s) 335, 2953, 3072, 3574, 3704, 3830
AflII CTTAAG 3 cut(s) 76, 152, 244
AflIII ACRYGT 4 cut(s) 1333, 2515, 3136, 3554
AjiI CACGTC 2 cut(s) 2098, 3137
AjnI CCWGG 8 cut(s) 793, 998, 2279, 2386, 2712, 3365, 3526, 4099
AjuI GAANNNNNNNTTGG 2 cut(s) 4000, 4032
AleI CACNNNNGTG 1 cut(s) 555
AloI GAACNNNNNNTCC 4 cut(s) 1357, 1389, 1585, 1617
Alw21I GWGCWC 3 cut(s) 52, 1007, 1586
Alw26I GTCTC 4 cut(s) 913, 2088, 3032, 3674
Alw44I GTGCAC 1 cut(s) 1582
AlwI GGATC 5 cut(s) 144, 348, 1500, 2147, 3805
AlwNI CAGNNNCTG 1 cut(s) 3665
Aor13HI TCCGGA 4 cut(s) 440, 1573, 2536, 3772
AoxI GGCC 5 cut(s) 559, 2698, 2715, 2803, 4191
ApaLI GTGCAC 1 cut(s) 1582
ApeKI GCWGC 8 cut(s) 532, 585, 657, 1179, 1287, 1821, 3208, 3383
ArsI GACNNNNNNTTYG 8 cut(s) 1499, 1531, 2916, 2948, 3785, 3817, 3969, 4001
AseI ATTAAT 1 cut(s) 1863
Asp718I GGTACC 1 cut(s) 2104
AspLEI GCGC 2 cut(s) 6, 3548
AspS9I GGNCC 6 cut(s) 271, 1628, 1748, 2716, 3612, 3721
AsuC2I CCSGG 1 cut(s) 1960
AsuHPI GGTGA 4 cut(s) 317, 1574, 1906, 4089
AsuNHI GCTAGC 1 cut(s) 3472
AvaII GGWCC 5 cut(s) 271, 1628, 1748, 3612, 3721
BaeGI GKGCMC 2 cut(s) 1586, 3067
BaeI ACNNNNGTAYC 2 cut(s) 3441, 3474
BalI TGGCCA 2 cut(s) 2700, 2805
BanI GGYRCC 1 cut(s) 2104
BanII GRGCYC 1 cut(s) 2214
BauI CACGAG 1 cut(s) 1815
BbrPI CACGTG 2 cut(s) 1982, 2033
BbsI GAAGAC 2 cut(s) 596, 1641
Bbv12I GWGCWC 3 cut(s) 52, 1007, 1586
BbvI GCAGC 8 cut(s) 519, 572, 644, 1166, 1299, 1808, 3195, 3370
BceAI ACGGC 3 cut(s) 534, 2604, 4095
BcgI CGANNNNNNTGC 2 cut(s) 3281, 3315
BciT130I CCWGG 8 cut(s) 795, 1000, 2281, 2388, 2714, 3367, 3528, 4101
BciVI GTATCC 1 cut(s) 1902
BclI TGATCA 1 cut(s) 948
BcnI CCSGG 1 cut(s) 1960
BcoDI GTCTC 4 cut(s) 913, 2088, 3032, 3674
BfaI CTAG 9 cut(s) 212, 353, 386, 689, 1148, 2912, 3086, 3212, 3473
BfmI CTRYAG 3 cut(s) 809, 2959, 3058
BfrI CTTAAG 3 cut(s) 76, 152, 244
BfuAI ACCTGC 2 cut(s) 700, 2116
BfuI GTATCC 1 cut(s) 1902
Bme1390I CCNGG 9 cut(s) 795, 1000, 1960, 2281, 2388, 2714, 3367, 3528, 4101
Bme18I GGWCC 5 cut(s) 271, 1628, 1748, 3612, 3721
BmgBI CACGTC 2 cut(s) 2098, 3137
BmgT120I GGNCC 6 cut(s) 271, 1628, 1748, 2716, 3612, 3721
BmiI GGNNCC 5 cut(s) 272, 273, 2106, 2618, 3364
BmrFI CCNGG 9 cut(s) 795, 1000, 1960, 2281, 2388, 2714, 3367, 3528, 4101
BmtI GCTAGC 1 cut(s) 3476
BpiI GAAGAC 2 cut(s) 596, 1641
BpmI CTGGAG 3 cut(s) 1021, 3349, 3825
Bpu10I CCTNAGC 1 cut(s) 1947
BpuEI CTTGAG 6 cut(s) 302, 574, 1228, 3370, 3553, 3882
BpuMI CCSGG 1 cut(s) 1960
Bsa29I ATCGAT 2 cut(s) 2340, 3633
BsaAI YACGTR 4 cut(s) 1653, 1982, 2033, 3453
BsaBI GATNNNNATC 2 cut(s) 542, 2163
BsaHI GRCGYC 1 cut(s) 2233
BsaJI CCNNGG 5 cut(s) 2279, 2522, 2712, 2893, 3526
BsaWI WCCGGW 5 cut(s) 133, 440, 1573, 2536, 3772
BsaXI ACNNNNNCTCC 4 cut(s) 1009, 1039, 3347, 3377
Bsc4I CCNNNNNNNGG 6 cut(s) 335, 2953, 3072, 3574, 3704, 3830
Bse118I RCCGGY 2 cut(s) 3567, 4140
Bse3DI GCAATG 3 cut(s) 1613, 2233, 2776
Bse8I GATNNNNATC 2 cut(s) 542, 2163
BseAI TCCGGA 4 cut(s) 440, 1573, 2536, 3772
BseBI CCWGG 8 cut(s) 795, 1000, 2281, 2388, 2714, 3367, 3528, 4101
BseCI ATCGAT 2 cut(s) 2340, 3633
BseDI CCNNGG 5 cut(s) 2279, 2522, 2712, 2893, 3526
BseJI GATNNNNATC 2 cut(s) 542, 2163
BseLI CCNNNNNNNGG 6 cut(s) 335, 2953, 3072, 3574, 3704, 3830
BseMI GCAATG 3 cut(s) 1613, 2233, 2776
BseRI GAGGAG 5 cut(s) 1071, 2796, 3160, 3593, 3629
BseSI GKGCMC 2 cut(s) 1586, 3067
BseXI GCAGC 8 cut(s) 519, 572, 644, 1166, 1299, 1808, 3195, 3370
BsgI GTGCAG 1 cut(s) 1306
BshFI GGCC 5 cut(s) 561, 2700, 2717, 2805, 4193
BshNI GGYRCC 1 cut(s) 2104
BshVI ATCGAT 2 cut(s) 2340, 3633
BsiHKAI GWGCWC 3 cut(s) 52, 1007, 1586
BsiSI CCGG 8 cut(s) 134, 441, 1574, 1960, 2537, 3568, 3773, 4141
BslFI GGGAC 5 cut(s) 257, 529, 3150, 3273, 3851
BslI CCNNNNNNNGG 6 cut(s) 335, 2953, 3072, 3574, 3704, 3830
BsmAI GTCTC 4 cut(s) 913, 2088, 3032, 3674
BsmBI CGTCTC 2 cut(s) 2088, 3674
BsmFI GGGAC 5 cut(s) 257, 529, 3150, 3273, 3851
BsmI GAATGC 1 cut(s) 2461
BsnI GGCC 5 cut(s) 561, 2700, 2717, 2805, 4193
Bsp1286I GDGCHC 5 cut(s) 52, 1007, 1586, 2214, 3067
Bsp13I TCCGGA 4 cut(s) 440, 1573, 2536, 3772
Bsp1407I TGTACA 2 cut(s) 2672, 3652
BspANI GGCC 5 cut(s) 561, 2700, 2717, 2805, 4193
BspDI ATCGAT 2 cut(s) 2340, 3633
BspEI TCCGGA 4 cut(s) 440, 1573, 2536, 3772
BspHI TCATGA 1 cut(s) 1504
BspLI GGNNCC 5 cut(s) 272, 273, 2106, 2618, 3364
BspMAI CTGCAG 1 cut(s) 813
BspMI ACCTGC 2 cut(s) 700, 2116
BspOI GCTAGC 1 cut(s) 3476
BspPI GGATC 5 cut(s) 144, 348, 1500, 2147, 3805
BspQI GCTCTTC 1 cut(s) 2175
BspT107I GGYRCC 1 cut(s) 2104
BspTI CTTAAG 3 cut(s) 76, 152, 244
BsrBI CCGCTC 1 cut(s) 286
BsrDI GCAATG 3 cut(s) 1613, 2233, 2776
BsrFI RCCGGY 2 cut(s) 3567, 4140
BsrGI TGTACA 2 cut(s) 2672, 3652
BssAI RCCGGY 2 cut(s) 3567, 4140
BssECI CCNNGG 5 cut(s) 2279, 2522, 2712, 2893, 3526
BssNAI GTATAC 2 cut(s) 2909, 4138
BssNI GRCGYC 1 cut(s) 2233
BssSI CACGAG 1 cut(s) 1815
BssT1I CCWWGG 1 cut(s) 2893
Bst1107I GTATAC 2 cut(s) 2909, 4138
Bst2BI CACGAG 1 cut(s) 1815
Bst2UI CCWGG 8 cut(s) 795, 1000, 2281, 2388, 2714, 3367, 3528, 4101
Bst4CI ACNGT 5 cut(s) 783, 2396, 2544, 3072, 3331
Bst6I CTCTTC 3 cut(s) 2175, 3071, 4149
BstACI GRCGYC 1 cut(s) 2233
BstAFI CTTAAG 3 cut(s) 76, 152, 244
BstAUI TGTACA 2 cut(s) 2672, 3652
BstBAI YACGTR 4 cut(s) 1653, 1982, 2033, 3453
BstC8I GCNNGC 4 cut(s) 198, 1145, 1345, 3474
BstDSI CCRYGG 1 cut(s) 2522
BstHHI GCGC 2 cut(s) 6, 3548
BstMAI GTCTC 4 cut(s) 913, 2088, 3032, 3674
BstMWI GCNNNNNNNGC 6 cut(s) 1149, 1267, 1964, 2209, 3866, 4079
BstNI CCWGG 8 cut(s) 795, 1000, 2281, 2388, 2714, 3367, 3528, 4101
BstNSI RCATGY 3 cut(s) 2519, 3015, 3558
BstSCI CCNGG 9 cut(s) 793, 998, 1958, 2279, 2386, 2712, 3365, 3526, 4099
BstSFI CTRYAG 3 cut(s) 809, 2959, 3058
BstSLI GKGCMC 2 cut(s) 1586, 3067
BstSNI TACGTA 1 cut(s) 3453
BstV1I GCAGC 8 cut(s) 519, 572, 644, 1166, 1299, 1808, 3195, 3370
BstV2I GAAGAC 2 cut(s) 596, 1641
BstX2I RGATCY 2 cut(s) 340, 3810
BstXI CCANNNNNNTGG 2 cut(s) 412, 4041
BstYI RGATCY 2 cut(s) 340, 3810
BstZ17I GTATAC 2 cut(s) 2909, 4138
Bsu15I ATCGAT 2 cut(s) 2340, 3633
BsuI GTATCC 1 cut(s) 1902
BsuRI GGCC 5 cut(s) 561, 2700, 2717, 2805, 4193
BsuTUI ATCGAT 2 cut(s) 2340, 3633
BtgI CCRYGG 1 cut(s) 2522
BtrI CACGTC 2 cut(s) 2098, 3137
BveI ACCTGC 2 cut(s) 700, 2116
Cac8I GCNNGC 4 cut(s) 198, 1145, 1345, 3474
CaiI CAGNNNCTG 1 cut(s) 3665
CciI TCATGA 1 cut(s) 1504
CfoI GCGC 2 cut(s) 6, 3548
Cfr10I RCCGGY 2 cut(s) 3567, 4140
Cfr13I GGNCC 6 cut(s) 271, 1628, 1748, 2716, 3612, 3721
ClaI ATCGAT 2 cut(s) 2340, 3633
CseI GACGC 6 cut(s) 601, 1940, 1985, 2024, 3755, 4118
CsiI ACCWGGT 2 cut(s) 2386, 4099
DraI TTTAAA 3 cut(s) 579, 1329, 2124
DraIII CACNNNGTG 2 cut(s) 1590, 2318
EaeI YGGCCR 3 cut(s) 2698, 2803, 4191
Eam1104I CTCTTC 3 cut(s) 2175, 3071, 4149
EarI CTCTTC 3 cut(s) 2175, 3071, 4149
EciI GGCGGA 1 cut(s) 3723
Eco105I TACGTA 1 cut(s) 3453
Eco130I CCWWGG 1 cut(s) 2893
Eco24I GRGCYC 1 cut(s) 2214
Eco32I GATATC 3 cut(s) 540, 630, 2161
Eco47I GGWCC 5 cut(s) 271, 1628, 1748, 3612, 3721
Eco57I CTGAAG 6 cut(s) 247, 656, 788, 1280, 1383, 3303
Eco72I CACGTG 2 cut(s) 1982, 2033
EcoO109I RGGNCCY 2 cut(s) 271, 3721
EcoRI GAATTC 4 cut(s) 818, 1497, 1569, 1897
EcoRII CCWGG 8 cut(s) 793, 998, 2279, 2386, 2712, 3365, 3526, 4099
EcoRV GATATC 3 cut(s) 540, 630, 2161
EcoT14I CCWWGG 1 cut(s) 2893
EcoT22I ATGCAT 2 cut(s) 2463, 2532
EcoT38I GRGCYC 1 cut(s) 2214
ErhI CCWWGG 1 cut(s) 2893
Esp3I CGTCTC 2 cut(s) 2088, 3674
FaqI GGGAC 5 cut(s) 257, 529, 3150, 3273, 3851
FauI CCCGC 3 cut(s) 893, 1817, 2606
FauNDI CATATG 1 cut(s) 2725
FbaI TGATCA 1 cut(s) 948
FblI GTMKAC 4 cut(s) 2044, 2908, 3232, 4137
FriOI GRGCYC 1 cut(s) 2214
FspBI CTAG 9 cut(s) 212, 353, 386, 689, 1148, 2912, 3086, 3212, 3473
GlaI GCGC 2 cut(s) 5, 3547
GsuI CTGGAG 3 cut(s) 1021, 3349, 3825
HaeIII GGCC 5 cut(s) 561, 2700, 2717, 2805, 4193
HapII CCGG 8 cut(s) 134, 441, 1574, 1960, 2537, 3568, 3773, 4141
HgaI GACGC 6 cut(s) 601, 1940, 1985, 2024, 3755, 4118
HhaI GCGC 2 cut(s) 6, 3548
Hin1I GRCGYC 1 cut(s) 2233
Hin6I GCGC 2 cut(s) 4, 3546
HinP1I GCGC 2 cut(s) 4, 3546
HincII GTYRAC 7 cut(s) 131, 400, 1541, 2045, 2501, 2547, 3233
HindII GTYRAC 7 cut(s) 131, 400, 1541, 2045, 2501, 2547, 3233
HindIII AAGCTT 1 cut(s) 1268
HpaII CCGG 8 cut(s) 134, 441, 1574, 1960, 2537, 3568, 3773, 4141
HphI GGTGA 4 cut(s) 317, 1574, 1906, 4089
Hpy99I CGWCG 3 cut(s) 617, 3007, 3234
HpyCH4III ACNGT 5 cut(s) 783, 2396, 2544, 3072, 3331
HpyF10VI GCNNNNNNNGC 6 cut(s) 1149, 1267, 1964, 2209, 3866, 4079
Hsp92I GRCGYC 1 cut(s) 2233
HspAI GCGC 2 cut(s) 4, 3546
KflI GGGWCCC 1 cut(s) 271
Kpn2I TCCGGA 4 cut(s) 440, 1573, 2536, 3772
KpnI GGTACC 1 cut(s) 2108
Ksp22I TGATCA 1 cut(s) 948
LguI GCTCTTC 1 cut(s) 2175
LmnI GCTCC 7 cut(s) 55, 988, 1002, 1157, 1939, 2616, 3368
Lsp1109I GCAGC 8 cut(s) 519, 572, 644, 1166, 1299, 1808, 3195, 3370
MabI ACCWGGT 2 cut(s) 2386, 4099
MaeI CTAG 9 cut(s) 212, 353, 386, 689, 1148, 2912, 3086, 3212, 3473
MbiI CCGCTC 1 cut(s) 286
MfeI CAATTG 3 cut(s) 333, 2592, 2694
MflI RGATCY 2 cut(s) 340, 3810
MhlI GDGCHC 5 cut(s) 52, 1007, 1586, 2214, 3067
MlsI TGGCCA 2 cut(s) 2700, 2805
MluNI TGGCCA 2 cut(s) 2700, 2805
MlyI GAGTC 7 cut(s) 247, 1791, 2542, 2924, 3034, 3228, 4076
MmeI TCCRAC 2 cut(s) 457, 571
Mox20I TGGCCA 2 cut(s) 2700, 2805
Mph1103I ATGCAT 2 cut(s) 2463, 2532
MroI TCCGGA 4 cut(s) 440, 1573, 2536, 3772
MscI TGGCCA 2 cut(s) 2700, 2805
MslI CAYNNNNRTG 3 cut(s) 555, 859, 3792
Msp20I TGGCCA 2 cut(s) 2700, 2805
MspA1I CMGCKG 1 cut(s) 1843
MspCI CTTAAG 3 cut(s) 76, 152, 244
MspI CCGG 8 cut(s) 134, 441, 1574, 1960, 2537, 3568, 3773, 4141
MspR9I CCNGG 9 cut(s) 795, 1000, 1960, 2281, 2388, 2714, 3367, 3528, 4101
MunI CAATTG 3 cut(s) 333, 2592, 2694
Mva1269I GAATGC 1 cut(s) 2461
MvaI CCWGG 8 cut(s) 795, 1000, 2281, 2388, 2714, 3367, 3528, 4101
MwoI GCNNNNNNNGC 6 cut(s) 1149, 1267, 1964, 2209, 3866, 4079
NciI CCSGG 1 cut(s) 1960
NdeI CATATG 1 cut(s) 2725
NheI GCTAGC 1 cut(s) 3472
NlaIV GGNNCC 5 cut(s) 272, 273, 2106, 2618, 3364
NmeAIII GCCGAG 1 cut(s) 3684
NmuCI GTSAC 5 cut(s) 1070, 1788, 2318, 2396, 3344
NsiI ATGCAT 2 cut(s) 2463, 2532
NspI RCATGY 3 cut(s) 2519, 3015, 3558
OliI CACNNNNGTG 1 cut(s) 555
PagI TCATGA 1 cut(s) 1504
PaqCI CACCTGC 1 cut(s) 700
PciI ACATGT 2 cut(s) 2515, 3554
PciSI GCTCTTC 1 cut(s) 2175
PctI GAATGC 1 cut(s) 2461
PfeI GAWTC 8 cut(s) 110, 827, 1097, 2131, 2414, 2990, 3296, 3892
PflMI CCANNNNNTGG 2 cut(s) 335, 3830
PfoI TCCNGGA 1 cut(s) 793
PleI GAGTC 7 cut(s) 247, 1791, 2542, 2923, 3034, 3228, 4075
PmaCI CACGTG 2 cut(s) 1982, 2033
PmlI CACGTG 2 cut(s) 1982, 2033
PpsI GAGTC 7 cut(s) 247, 1791, 2542, 2923, 3034, 3228, 4075
Ppu21I YACGTR 4 cut(s) 1653, 1982, 2033, 3453
PpuMI RGGWCCY 2 cut(s) 271, 3721
PscI ACATGT 2 cut(s) 2515, 3554
PshBI ATTAAT 1 cut(s) 1863
Psp5II RGGWCCY 2 cut(s) 271, 3721
Psp6I CCWGG 8 cut(s) 793, 998, 2279, 2386, 2712, 3365, 3526, 4099
PspCI CACGTG 2 cut(s) 1982, 2033
PspGI CCWGG 8 cut(s) 793, 998, 2279, 2386, 2712, 3365, 3526, 4099
PspN4I GGNNCC 5 cut(s) 272, 273, 2106, 2618, 3364
PspPI GGNCC 6 cut(s) 271, 1628, 1748, 2716, 3612, 3721
PspPPI RGGWCCY 2 cut(s) 271, 3721
PstI CTGCAG 1 cut(s) 813
PstNI CAGNNNCTG 1 cut(s) 3665
PsuI RGATCY 2 cut(s) 340, 3810
PvuII CAGCTG 1 cut(s) 1843
RseI CAYNNNNRTG 3 cut(s) 555, 859, 3792
SalI GTCGAC 2 cut(s) 2043, 3231
SapI GCTCTTC 1 cut(s) 2175
Sau96I GGNCC 6 cut(s) 271, 1628, 1748, 2716, 3612, 3721
SchI GAGTC 7 cut(s) 247, 1791, 2542, 2924, 3034, 3228, 4076
ScrFI CCNGG 9 cut(s) 795, 1000, 1960, 2281, 2388, 2714, 3367, 3528, 4101
SduI GDGCHC 5 cut(s) 52, 1007, 1586, 2214, 3067
SexAI ACCWGGT 2 cut(s) 2386, 4099
SfcI CTRYAG 3 cut(s) 809, 2959, 3058
SinI GGWCC 5 cut(s) 271, 1628, 1748, 3612, 3721
SmiMI CAYNNNNRTG 3 cut(s) 555, 859, 3792
SmlI CTYRAG 9 cut(s) 76, 152, 244, 281, 553, 1207, 3349, 3532, 3897
SmoI CTYRAG 9 cut(s) 76, 152, 244, 281, 553, 1207, 3349, 3532, 3897
SnaBI TACGTA 1 cut(s) 3453
SspI AATATT 3 cut(s) 1109, 2868, 3104
SspMI CTAG 9 cut(s) 212, 353, 386, 689, 1148, 2912, 3086, 3212, 3473
StyD4I CCNGG 9 cut(s) 793, 998, 1958, 2279, 2386, 2712, 3365, 3526, 4099
StyI CCWWGG 1 cut(s) 2893
TaaI ACNGT 5 cut(s) 783, 2396, 2544, 3072, 3331
TatI WGTACW 3 cut(s) 2509, 2672, 3652
TauI GCSGC 2 cut(s) 171, 289
TfiI GAWTC 8 cut(s) 110, 827, 1097, 2131, 2414, 2990, 3296, 3892
TseFI GTSAC 5 cut(s) 1070, 1788, 2318, 2396, 3344
TseI GCWGC 8 cut(s) 532, 585, 657, 1179, 1287, 1821, 3208, 3383
Tsp45I GTSAC 5 cut(s) 1070, 1788, 2318, 2396, 3344
TspGWI ACGGA 4 cut(s) 1019, 2539, 2753, 3422
Van91I CCANNNNNTGG 2 cut(s) 335, 3830
Vha464I CTTAAG 3 cut(s) 76, 152, 244
VneI GTGCAC 1 cut(s) 1582
VpaK11BI GGWCC 5 cut(s) 271, 1628, 1748, 3612, 3721
VspI ATTAAT 1 cut(s) 1863
XbaI TCTAGA 1 cut(s) 385
XceI RCATGY 3 cut(s) 2519, 3015, 3558
XcmI CCANNNNNNNNNTGG 2 cut(s) 554, 3310
XmiI GTMKAC 4 cut(s) 2044, 2908, 3232, 4137
XspI CTAG 9 cut(s) 212, 353, 386, 689, 1148, 2912, 3086, 3212, 3473
ZraI GACGTC 1 cut(s) 2234
Zsp2I ATGCAT 2 cut(s) 2463, 2532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.