pycom11g18370

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000307
Physical Location & Seq
Forward (+)
20527939 .. 20531948
4010 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g18370.4

Sequence Viewer

Length: 3099 bp
ATGGAAAAGTTGTCACGACTTTATTTAGATGGAACAGCTATAAGAGAGCTGCCGGTAACATTCGAGAAGTTGAGAGGTCTTATTTTGTTAAATCTAAAAGGCTGCAAGAACCTTCATTTGAGTCGTTCAAGCATCCTTGGTAGTTTGACATCTCTGAAAGATCTCAATTTGTCAGGCTGCTCACATGTTGAACTGCCTGAAAACATAGGGAACTTGCAACATTTGGAGGAGCTTGATGCCTGTGACAGCGGCATAAGAAAAGTTCCCTCCTCCATTTCACGTCTGAATAAGCTTAAACTATTATGTTTAAATAGATGTAAAGATGAGCAAATGGGTTTGCAGTTGCCTGACTCTTTCTCAGGTTTGACCTCATTGTATAGATTAAATCTAGGTCAATGCGGTCTTGAGGAAGGAGCCATCCCTGATGATATTCACTGCTTATCGTCATTGCTGTATTTGGAATTGAGAGAAAACAATTTTGTGAGCTTACCGGCAGGCATCTCTCAACTTCCTGAGCTTAAACAACTTGCCTTGGCTAGGTGCAGAAATCTAGAGTCGTTACCAAAAACTCTTCCATCCAAACTAACAGACATAGAAGCTCGTGAGTGCCCAATGTTGAAAAATTATTCAAATAATTTGACAATACGGGCTGATGGCGGTTTCTGTCACATAGATTGTGGAAACCCTTGCGAGAATGAAGAGAACGAAGACTATTGGGCGAACCATTTTCCACTTCCAATTCCAGAAGACCATGCTGAAACACACCTTCCTAAGTACATAGAGGAGGGAATAAAAAATGAAGAGCCATTTCAATTTCGTTTTCCTCACAGTGCTATAATTCCCGACTGGTGTGGTGAACATTCAAGTCGGCATTCTGTAACTGTCCAACTATCTGATAGCACGTGGATAGGGTTTGCCCTGTTTGTTGTTTTTGAACTTCTCGTGAAGGAAGATTTTGATAACAGTTGGGAAATGGAAGACACTTTTTGCCATTTTAGCACCCCGGAAGGTCGACTTAAAGATCCTCTAGTCCTTGAAAAGTTTAATAATTTCAGAAAGGGGTCGTATGGACTTTGTTGCTATGAGCCACGAGGCGGGCAATTTGCAGGGCAATTGAATAAGGAAAGTAGGCTACTTAAAGCTTCAGTTTCAACGAATAGACCTGATCGATCGCTGATAGTAAAAGGGTGCGCATTACAATTAATATACCAAAAAGATGCTGCAGCGTTTGCTCACAATTTATCAGAATCTGCTAATGGACATCTGAATTTGGATTTTGGTCGACATTGTAAGAGAATATTGGATCGGGCAAGGGAACTGAAAAACTCTGGTGAGGTGACAGTAATGGGCTCTAATCTACAAGAGCTAGGATCAACTTCAACTTCAAATCAAGGCAGCAGTAGCCAGGGTACTACTTACTCTAACATCGAGCAGTCAAATGGGGAGTTAGAGCTGTTGGTTTCAGCACTGTATGAGGGATGCAATGGACGCGAGAAGAATTTTCATTTTATTTTTTCTCATCCTATAATTTCACGCTGGTTCATCCATCACTATGCCGGAGGCGTAGCACTTTATCGCCTTCCTCAACACTTATATGATGACAAGAGATGGGTAGGATTCGAACTATATGTTGTCCTTACCTGGTCTTCATCTGATTACAACAATCCTCCCCATTCTGCACGGCTCCATGTTGATTTGTGTTCCCCTGAAAGTCGTATAATGCATAGGTGCATCAAAACACACAGTTGCCTTGGACAAAAGCAACTAGTTGTTTTGCACATCCCACGTGCGTGTTTTTCACAACACGAGTTGGATCAATGTCAAGGTGTTGGCGCTTTGTGTAGAACCAGTACCCCAGACGTGGAAGTCGAAGTGTGCGGGAGCCGTCTGGTATATGAGCAAGACTTGAAAGAGTTAATCAAAGCATTAGCAACTGAATGCACATTCAAGGGACCACTCCACCTACTTTATGTGCAGGCACGAACATATGTTGAAAACATGGTGGAAAGTCCACTTCGTGAAGCTTTAACGGTTCAACGTCAAGCACTAGTACCTCAATTCAGAGAGATTTGGGAAGTTTGTAAATTGTTCTGGCAAGATCTGCTTCTGAACTCAGGTCCTGAAGAACAAGTAGATGAACAAGATTTCACTTCAAGATTGGACTACTACATGTGCTATCTCGAACAAGAGCAAGTCCTTGTGGAACCACTTGACCATCTACTTGTCCATTCCAAACGCTATCTTTTGGAAAATAGGGATTCCATCCAACAACATGATGCGGAAAGATATTCAGAATCATTGGATAGACGGTTATACATCATGGCTGAGAATCAACTCTATGGAAGCTGTAATATAGCTGCGTGGAAAAAAAGTATTGTGGTGTTGCTTCAAATTTGGGAGGAGGCCATTCTCAGTCTCAAAGGACATACAATATCTGTTTTGAAGCTTTTCGATCCTTCCGCACCATACAACTTTTGTTTCCCTCAGAAGAAAATTCTAGAATGGTTTAAAAATCAGACTAGTAAGTCATCGACGATCGGAATGGAACTACCTCAGGGTCTGTATGATGATGATAATTGGAGGGGACTTGTGACATGTGCTGCTTTTTCAGTCCATGAGCTTCCAACTGCTACCCTTGAAAAACTGGGTTCAGAAATGACTGTCGAACTTATGTGTCACTTAAGTACTGAGGAGTATTGCTTGAACCCTATTCCCATTTGTTCCACAACTAAAGAGAAATTCAAGTGGTTGCATCTCCGCGGATTTATCTGGCTGACTTACATACCCCGAATTTTCCTGGCAGAACTCAAGGACATACCACTTGTACTAGCCAGAATTTATCATACTTGCCCAGGCTTGACTGTCGAGGATACTGGTATTCGTCTCTTATACAAGCACGAAGTGGAGGAGCTAAAGCAATCAATGACCCAGTGCTGGACCTCCTTCTTTGACAATTCAGACCTCATCCGTCAATTCGTAGAAGAAGATGAATTATTCAGTCGAGTGGTTGATGAATTTGAACAAGGAACGACGTTCGGAGGACATATCAAGGACCATGGATCCATCGGGACTTTCATAAGCATTCTCCCAGGCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

1033

Amino Acids

117.63

Weight (kDa)

5.57

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g16600 FvH4_1g16610 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16620 FvH4_1g16621 FvH4_1g16630 FvH4_1g16640 FvH4_1g16640 FvH4_1g16650 FvH4_1g16650 FvH4_3g27631 FvH4_4g11392 FvH4_4g11542
prunus_persica Prupe.4G224500_v2.0.a1 Prupe.4G226900_v2.0.a1 Prupe.4G227000_v2.0.a1
pyrus_communis pycom11g18180 pycom11g18210 pycom11g18230 pycom11g18270 pycom11g18350 pycom11g18360 pycom11g18370
rosa_chinensis RchiOBHm_Chr2g0106511 RchiOBHm_Chr2g0106541 RchiOBHm_Chr2g0106551 RchiOBHm_Chr2g0106571 RchiOBHm_Chr2g0124621 RchiOBHm_Chr6g0251591 RchiOBHm_Chr6g0251631 RchiOBHm_Chr6g0251641
rosa_laevigata RLG00000015443 RLG00000015444 RLG00000015458 RLG00000017510 RLG00000017511 RLG00000017512 RLG00000017515 RLG00000017516 RLG00000017517 RLG00000018809 RLG00000020934 RLG00000020935 RLG00000034092
rosa_multiflora Rmu_co8285777.1_g000001 Rmu_sc0000762.1_g000001 Rmu_sc0001872.1_g000006 Rmu_sc0005255.1_g000019 Rmu_sc0005597.1_g000006 Rmu_sc0012104.1_g000001 Rmu_sc0017105.1_g000011 Rmu_sc0022904.1_g000001 Rmu_ssc0000135.1_g000049 Rmu_ssc0000213.1_g000047
rosa_roxburghii Rroxscaffold_2G00092830 Rroxscaffold_2G00119790 Rroxscaffold_2G00137010 Rroxscaffold_2G00137030 Rroxscaffold_2G00137070 Rroxscaffold_7G00216560
rosa_rugosa Rorug02G0136100 Rorug02G0136200 Rorug02G0136300 Rorug02G0136400 Rorug02G0251800 Rorug02G0251800 Rorug02G0455500 Rorug02G0455600 Rorug02G0455700 Rorug05G0195300
rosa_samantha Rh2AG187400 Rh2AG187500 Rh2AG187600 Rh2AG310500 Rh2BG198200 Rh2BG198300 Rh2BG198400 Rh2BG198600 Rh2BG318900 Rh2BG534300 Rh2CG192400 Rh2CG192500 Rh2CG192600 Rh2CG297500 Rh2DG193500 Rh2DG193600 Rh2DG193800 Rh2DG334200 Rh2DG543100 Rh5AG280400 Rh5BG285800 Rh5BG285900 Rh5CG317900 Rh5CG318000 Rh5DG294600 Rh5DG294700 Rh6AG012200 Rh6AG012700 Rh6BG011200 Rh6BG011700
rosa_wichuraiana Rw0G009380 Rw2G014610 Rw2G014630 Rw2G014640 Rw2G014680 Rw2G025120 Rw2G043000 Rw5G026490 Rw6G001370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1865, 2524
Acc16I TGCGCA 1 cut(s) 1192
AccI GTMKAC 2 cut(s) 1012, 1282
AccII CGCG 2 cut(s) 1491, 2760
AciI CCGC 9 cut(s) 249, 399, 657, 1095, 1878, 2279, 2460, 2758, 2760
AclWI GGATC 7 cut(s) 1016, 1311, 1378, 1821, 2447, 3054, 3067
AcsI RAATTY 8 cut(s) 1267, 1498, 2391, 2493, 2738, 2790, 2835, 3014
AcuI CTGAAG 2 cut(s) 1128, 2142
AcvI CACGTG 2 cut(s) 903, 1787
AdeI CACNNNGTG 2 cut(s) 2018, 2902
AfaI GTAC 6 cut(s) 776, 1411, 1852, 2052, 2686, 2826
AfiI CCNNNNNNNGG 4 cut(s) 537, 1094, 1861, 2934
AflII CTTAAG 1 cut(s) 2680
AflIII ACRYGT 3 cut(s) 184, 2169, 2594
AhlI ACTAGT 3 cut(s) 1765, 2047, 2519
AjiI CACGTC 2 cut(s) 281, 1861
AjnI CCWGG 5 cut(s) 1404, 1640, 2796, 2851, 3088
AleI CACNNNNGTG 1 cut(s) 1789
Alw26I GTCTC 2 cut(s) 2420, 2888
AlwI GGATC 7 cut(s) 1016, 1311, 1378, 1821, 2447, 3054, 3067
AlwNI CAGNNNCTG 3 cut(s) 1250, 2120, 2560
AoxI GGCC 1 cut(s) 2403
ApeKI GCWGC 8 cut(s) 49, 102, 177, 1220, 1223, 1395, 2357, 2600
ApoI RAATTY 8 cut(s) 1267, 1498, 2391, 2493, 2738, 2790, 2835, 3014
AseI ATTAAT 1 cut(s) 1202
Asp700I GAANNNNTTC 1 cut(s) 2447
AspLEI GCGC 2 cut(s) 1193, 1835
AspS9I GGNCC 4 cut(s) 1952, 2117, 2937, 3052
AsuC2I CCSGG 1 cut(s) 1004
AsuHPI GGTGA 3 cut(s) 866, 1343, 1348
AsuII TTCGAA 1 cut(s) 1620
AvaII GGWCC 4 cut(s) 1952, 2117, 2937, 3052
AxyI CCTNAGG 1 cut(s) 2553
BaeGI GKGCMC 1 cut(s) 611
BamHI GGATCC 1 cut(s) 3059
BanII GRGCYC 1 cut(s) 1352
BauI CACGAG 4 cut(s) 600, 941, 1089, 1805
BbrPI CACGTG 2 cut(s) 903, 1787
BbsI GAAGAC 4 cut(s) 714, 753, 984, 1638
BbvI GCAGC 8 cut(s) 36, 89, 164, 1207, 1235, 1407, 2344, 2587
BccI CCATC 9 cut(s) 23, 425, 583, 647, 1554, 1602, 2223, 2270, 3071
BceAI ACGGC 2 cut(s) 1697, 1869
BciT130I CCWGG 5 cut(s) 1406, 1642, 2798, 2853, 3090
BciVI GTATCC 1 cut(s) 2863
BcnI CCSGG 1 cut(s) 1004
BcoDI GTCTC 2 cut(s) 2420, 2888
BcuI ACTAGT 3 cut(s) 1765, 2047, 2519
BfmI CTRYAG 1 cut(s) 1221
BfoI RGCGCY 1 cut(s) 1836
BfrI CTTAAG 1 cut(s) 2680
BfuI GTATCC 1 cut(s) 2863
BglII AGATCT 2 cut(s) 160, 2098
BisI GCNGC 9 cut(s) 50, 103, 178, 250, 1221, 1224, 1396, 2358, 2601
BlsI GCNGC 9 cut(s) 51, 104, 179, 251, 1222, 1225, 1397, 2359, 2602
BmcAI AGTACT 1 cut(s) 2686
Bme1390I CCNGG 6 cut(s) 1004, 1406, 1642, 2798, 2853, 3090
Bme18I GGWCC 4 cut(s) 1952, 2117, 2937, 3052
BmgBI CACGTC 2 cut(s) 281, 1861
BmgT120I GGNCC 4 cut(s) 1952, 2117, 2937, 3052
BmiI GGNNCC 6 cut(s) 415, 1685, 1883, 1953, 2205, 3061
BmrFI CCNGG 6 cut(s) 1004, 1406, 1642, 2798, 2853, 3090
BmrI ACTGGG 2 cut(s) 2654, 2923
BmsI GCATC 8 cut(s) 141, 226, 507, 1207, 1469, 1740, 2266, 2761
BmuI ACTGGG 2 cut(s) 2654, 2923
BpiI GAAGAC 4 cut(s) 714, 753, 984, 1638
Bpu10I CCTNAGC 1 cut(s) 513
Bpu14I TTCGAA 1 cut(s) 1620
BpuEI CTTGAG 2 cut(s) 425, 2792
BpuMI CCSGG 1 cut(s) 1004
Bsa29I ATCGAT 1 cut(s) 1168
BsaAI YACGTR 2 cut(s) 903, 1787
BsaJI CCNNGG 9 cut(s) 136, 531, 1002, 1405, 1750, 2758, 2851, 3055, 3088
Bsc4I CCNNNNNNNGG 4 cut(s) 537, 1094, 1861, 2934
Bse118I RCCGGY 2 cut(s) 52, 490
Bse1I ACTGG 5 cut(s) 851, 1848, 2649, 2878, 2929
Bse21I CCTNAGG 1 cut(s) 2553
Bse3DI GCAATG 2 cut(s) 446, 1489
BseBI CCWGG 5 cut(s) 1406, 1642, 2798, 2853, 3090
BseCI ATCGAT 1 cut(s) 1168
BseDI CCNNGG 9 cut(s) 136, 531, 1002, 1405, 1750, 2758, 2851, 3055, 3088
BseGI GGATG 9 cut(s) 132, 417, 575, 1484, 1519, 1542, 1779, 2262, 2964
BseLI CCNNNNNNNGG 4 cut(s) 537, 1094, 1861, 2934
BseMI GCAATG 2 cut(s) 446, 1489
BseMII CTCAG 8 cut(s) 372, 504, 2127, 2316, 2425, 2498, 2567, 2679
BseNI ACTGG 5 cut(s) 851, 1848, 2649, 2878, 2929
BseRI GAGGAG 6 cut(s) 242, 259, 797, 2414, 2705, 2921
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 8 cut(s) 36, 89, 164, 1207, 1235, 1407, 2344, 2587
BsgI GTGCAG 3 cut(s) 562, 1662, 1994
Bsh1236I CGCG 2 cut(s) 1491, 2760
Bsh1285I CGRYCG 2 cut(s) 1172, 2538
BshFI GGCC 1 cut(s) 2405
BshVI ATCGAT 1 cut(s) 1168
BsiEI CGRYCG 2 cut(s) 1172, 2538
BsiSI CCGG 4 cut(s) 53, 491, 1004, 1557
BslFI GGGAC 3 cut(s) 1965, 2598, 3082
BslI CCNNNNNNNGG 4 cut(s) 537, 1094, 1861, 2934
BsmAI GTCTC 2 cut(s) 2420, 2888
BsmBI CGTCTC 1 cut(s) 2888
BsmFI GGGAC 3 cut(s) 1965, 2598, 3082
BsmI GAATGC 3 cut(s) 871, 1943, 3081
BsnI GGCC 1 cut(s) 2405
Bsp119I TTCGAA 1 cut(s) 1620
Bsp1286I GDGCHC 2 cut(s) 611, 1352
Bsp19I CCATGG 1 cut(s) 3055
BspACI CCGC 9 cut(s) 249, 399, 657, 1095, 1878, 2279, 2460, 2758, 2760
BspANI GGCC 1 cut(s) 2405
BspCNI CTCAG 8 cut(s) 371, 505, 2126, 2317, 2424, 2497, 2566, 2680
BspDI ATCGAT 1 cut(s) 1168
BspFNI CGCG 2 cut(s) 1491, 2760
BspLI GGNNCC 6 cut(s) 415, 1685, 1883, 1953, 2205, 3061
BspMAI CTGCAG 1 cut(s) 1225
BspPI GGATC 7 cut(s) 1016, 1311, 1378, 1821, 2447, 3054, 3067
BspQI GCTCTTC 1 cut(s) 795
BspT104I TTCGAA 1 cut(s) 1620
BspTI CTTAAG 1 cut(s) 2680
BsrDI GCAATG 2 cut(s) 446, 1489
BsrFI RCCGGY 2 cut(s) 52, 490
BsrI ACTGG 5 cut(s) 851, 1848, 2649, 2878, 2929
BssAI RCCGGY 2 cut(s) 52, 490
BssECI CCNNGG 9 cut(s) 136, 531, 1002, 1405, 1750, 2758, 2851, 3055, 3088
BssSI CACGAG 4 cut(s) 600, 941, 1089, 1805
BssT1I CCWWGG 4 cut(s) 136, 531, 1750, 3055
Bst2BI CACGAG 4 cut(s) 600, 941, 1089, 1805
Bst2UI CCWGG 5 cut(s) 1406, 1642, 2798, 2853, 3090
Bst6I CTCTTC 3 cut(s) 576, 693, 795
BstAFI CTTAAG 1 cut(s) 2680
BstAPI GCANNNNNTGC 2 cut(s) 1229, 2101
BstBAI YACGTR 2 cut(s) 903, 1787
BstBI TTCGAA 1 cut(s) 1620
BstC8I GCNNGC 3 cut(s) 496, 1097, 1977
BstDEI CTNAG 9 cut(s) 358, 513, 771, 2113, 2325, 2411, 2484, 2553, 2688
BstDSI CCRYGG 2 cut(s) 2758, 3055
BstENI CCTNNNNNAGG 1 cut(s) 535
BstF5I GGATG 9 cut(s) 132, 417, 575, 1484, 1519, 1542, 1779, 2262, 2964
BstFNI CGCG 2 cut(s) 1491, 2760
BstH2I RGCGCY 1 cut(s) 1836
BstHHI GCGC 2 cut(s) 1193, 1835
BstMAI GTCTC 2 cut(s) 2420, 2888
BstMCI CGRYCG 2 cut(s) 1172, 2538
BstMWI GCNNNNNNNGC 5 cut(s) 996, 1229, 1401, 1488, 2101
BstNI CCWGG 5 cut(s) 1406, 1642, 2798, 2853, 3090
BstNSI RCATGY 3 cut(s) 188, 2173, 2598
BstSCI CCNGG 6 cut(s) 1002, 1404, 1640, 2796, 2851, 3088
BstSFI CTRYAG 1 cut(s) 1221
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 2 cut(s) 1491, 2760
BstV1I GCAGC 8 cut(s) 36, 89, 164, 1207, 1235, 1407, 2344, 2587
BstV2I GAAGAC 4 cut(s) 714, 753, 984, 1638
BstX2I RGATCY 4 cut(s) 160, 1021, 2098, 3059
BstYI RGATCY 4 cut(s) 160, 1021, 2098, 3059
Bsu15I ATCGAT 1 cut(s) 1168
Bsu36I CCTNAGG 1 cut(s) 2553
BsuI GTATCC 1 cut(s) 2863
BsuRI GGCC 1 cut(s) 2405
BsuTUI ATCGAT 1 cut(s) 1168
BtgI CCRYGG 2 cut(s) 2758, 3055
BtrI CACGTC 2 cut(s) 281, 1861
BtsCI GGATG 9 cut(s) 132, 417, 575, 1484, 1519, 1542, 1779, 2262, 2964
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 4 cut(s) 433, 835, 1466, 2936
Cac8I GCNNGC 3 cut(s) 496, 1097, 1977
CaiI CAGNNNCTG 3 cut(s) 1250, 2120, 2560
CfoI GCGC 2 cut(s) 1193, 1835
Cfr10I RCCGGY 2 cut(s) 52, 490
Cfr13I GGNCC 4 cut(s) 1952, 2117, 2937, 3052
Cfr42I CCGCGG 1 cut(s) 2761
ClaI ATCGAT 1 cut(s) 1168
CseI GACGC 1 cut(s) 1497
CsiI ACCWGGT 1 cut(s) 1640
Csp6I GTAC 6 cut(s) 775, 1410, 1851, 2051, 2685, 2825
CviQI GTAC 6 cut(s) 775, 1410, 1851, 2051, 2685, 2825
DdeI CTNAG 9 cut(s) 358, 513, 771, 2113, 2325, 2411, 2484, 2553, 2688
DraI TTTAAA 2 cut(s) 309, 2509
DraIII CACNNNGTG 2 cut(s) 2018, 2902
DrdI GACNNNNNNGTC 2 cut(s) 1865, 2524
DseDI GACNNNNNNGTC 2 cut(s) 1865, 2524
Eam1104I CTCTTC 3 cut(s) 576, 693, 795
EarI CTCTTC 3 cut(s) 576, 693, 795
Eco130I CCWWGG 4 cut(s) 136, 531, 1750, 3055
Eco24I GRGCYC 1 cut(s) 1352
Eco47I GGWCC 4 cut(s) 1952, 2117, 2937, 3052
Eco57I CTGAAG 2 cut(s) 1128, 2142
Eco72I CACGTG 2 cut(s) 903, 1787
Eco81I CCTNAGG 1 cut(s) 2553
EcoNI CCTNNNNNAGG 1 cut(s) 535
EcoO109I RGGNCCY 1 cut(s) 2117
EcoRII CCWGG 5 cut(s) 1404, 1640, 2796, 2851, 3088
EcoT14I CCWWGG 4 cut(s) 136, 531, 1750, 3055
EcoT22I ATGCAT 1 cut(s) 1725
EcoT38I GRGCYC 1 cut(s) 1352
ErhI CCWWGG 4 cut(s) 136, 531, 1750, 3055
Esp3I CGTCTC 1 cut(s) 2888
FalI AAGNNNNNCTT 4 cut(s) 999, 1031, 2088, 2120
FaqI GGGAC 3 cut(s) 1965, 2598, 3082
FauI CCCGC 2 cut(s) 1088, 1871
FauNDI CATATG 1 cut(s) 1987
FblI GTMKAC 2 cut(s) 1012, 1282
Fnu4HI GCNGC 9 cut(s) 50, 103, 178, 250, 1221, 1224, 1396, 2358, 2601
FokI GGATG 9 cut(s) 119, 404, 562, 1491, 1506, 1529, 1766, 2249, 2951
FriOI GRGCYC 1 cut(s) 1352
Fsp4HI GCNGC 9 cut(s) 50, 103, 178, 250, 1221, 1224, 1396, 2358, 2601
FspAI RTGCGCAY 1 cut(s) 1192
FspI TGCGCA 1 cut(s) 1192
GlaI GCGC 2 cut(s) 1192, 1834
GluI GCNGC 9 cut(s) 50, 103, 178, 250, 1221, 1224, 1396, 2358, 2601
HaeII RGCGCY 1 cut(s) 1836
HaeIII GGCC 1 cut(s) 2405
HapII CCGG 4 cut(s) 53, 491, 1004, 1557
HgaI GACGC 1 cut(s) 1497
HhaI GCGC 2 cut(s) 1193, 1835
Hin6I GCGC 2 cut(s) 1191, 1833
HinP1I GCGC 2 cut(s) 1191, 1833
HincII GTYRAC 2 cut(s) 1013, 1283
HindII GTYRAC 2 cut(s) 1013, 1283
HindIII AAGCTT 4 cut(s) 290, 1140, 2022, 2444
HinfI GANTC 8 cut(s) 121, 350, 554, 1247, 1617, 2258, 2294, 2329
HpaII CCGG 4 cut(s) 53, 491, 1004, 1557
HphI GGTGA 3 cut(s) 866, 1343, 1348
Hpy166II GTNNAC 4 cut(s) 857, 1013, 1283, 2012
Hpy8I GTNNAC 4 cut(s) 857, 1013, 1283, 2012
Hpy99I CGWCG 2 cut(s) 2536, 3034
HpyAV CCTTC 8 cut(s) 122, 404, 776, 940, 1001, 1589, 2466, 2953
HpyCH4IV ACGT 6 cut(s) 280, 902, 1786, 1860, 2038, 3032
HpyF10VI GCNNNNNNNGC 5 cut(s) 996, 1229, 1401, 1488, 2101
HpyF3I CTNAG 9 cut(s) 358, 513, 771, 2113, 2325, 2411, 2484, 2553, 2688
HpySE526I ACGT 6 cut(s) 280, 902, 1786, 1860, 2038, 3032
HspAI GCGC 2 cut(s) 1191, 1833
KspI CCGCGG 1 cut(s) 2761
LguI GCTCTTC 1 cut(s) 795
LmnI GCTCC 5 cut(s) 229, 413, 1689, 1881, 2908
Lsp1109I GCAGC 8 cut(s) 36, 89, 164, 1207, 1235, 1407, 2344, 2587
LweI GCATC 8 cut(s) 141, 226, 507, 1207, 1469, 1740, 2266, 2761
MabI ACCWGGT 1 cut(s) 1640
MaeII ACGT 6 cut(s) 280, 902, 1786, 1860, 2038, 3032
MaeIII GTNAC 9 cut(s) 12, 55, 242, 558, 665, 877, 1336, 2590, 2675
MfeI CAATTG 1 cut(s) 1112
MflI RGATCY 4 cut(s) 160, 1021, 2098, 3059
MhlI GDGCHC 2 cut(s) 611, 1352
MlyI GAGTC 3 cut(s) 130, 344, 563
MmeI TCCRAC 4 cut(s) 910, 1791, 2290, 2648
Mph1103I ATGCAT 1 cut(s) 1725
MroXI GAANNNNTTC 1 cut(s) 2447
MslI CAYNNNNRTG 3 cut(s) 1551, 1593, 1789
MspA1I CMGCKG 2 cut(s) 249, 2760
MspCI CTTAAG 1 cut(s) 2680
MspI CCGG 4 cut(s) 53, 491, 1004, 1557
MspR9I CCNGG 6 cut(s) 1004, 1406, 1642, 2798, 2853, 3090
MunI CAATTG 1 cut(s) 1112
Mva1269I GAATGC 3 cut(s) 871, 1943, 3081
MvaI CCWGG 5 cut(s) 1406, 1642, 2798, 2853, 3090
MvnI CGCG 2 cut(s) 1491, 2760
MwoI GCNNNNNNNGC 5 cut(s) 996, 1229, 1401, 1488, 2101
NciI CCSGG 1 cut(s) 1004
NcoI CCATGG 1 cut(s) 3055
NdeI CATATG 1 cut(s) 1987
NlaIV GGNNCC 6 cut(s) 415, 1685, 1883, 1953, 2205, 3061
NmuCI GTSAC 6 cut(s) 12, 242, 665, 1336, 2590, 2675
NsbI TGCGCA 1 cut(s) 1192
NsiI ATGCAT 1 cut(s) 1725
NspI RCATGY 3 cut(s) 188, 2173, 2598
NspV TTCGAA 1 cut(s) 1620
OliI CACNNNNGTG 1 cut(s) 1789
PciI ACATGT 3 cut(s) 184, 2169, 2594
PciSI GCTCTTC 1 cut(s) 795
PcsI WCGNNNNNNNCGW 1 cut(s) 1866
PctI GAATGC 3 cut(s) 871, 1943, 3081
PdmI GAANNNNTTC 1 cut(s) 2447
PfeI GAWTC 5 cut(s) 1247, 1617, 2258, 2294, 2329
PkrI GCNGC 9 cut(s) 51, 104, 179, 251, 1222, 1225, 1397, 2359, 2602
Ple19I CGATCG 2 cut(s) 1172, 2538
PleI GAGTC 3 cut(s) 129, 344, 562
PmaCI CACGTG 2 cut(s) 903, 1787
PmlI CACGTG 2 cut(s) 903, 1787
PpsI GAGTC 3 cut(s) 129, 344, 562
Ppu21I YACGTR 2 cut(s) 903, 1787
PpuMI RGGWCCY 1 cut(s) 2117
PscI ACATGT 3 cut(s) 184, 2169, 2594
PshBI ATTAAT 1 cut(s) 1202
Psp5II RGGWCCY 1 cut(s) 2117
Psp6I CCWGG 5 cut(s) 1404, 1640, 2796, 2851, 3088
PspCI CACGTG 2 cut(s) 903, 1787
PspGI CCWGG 5 cut(s) 1404, 1640, 2796, 2851, 3088
PspN4I GGNNCC 6 cut(s) 415, 1685, 1883, 1953, 2205, 3061
PspPI GGNCC 4 cut(s) 1952, 2117, 2937, 3052
PspPPI RGGWCCY 1 cut(s) 2117
PstI CTGCAG 1 cut(s) 1225
PstNI CAGNNNCTG 3 cut(s) 1250, 2120, 2560
PsuI RGATCY 4 cut(s) 160, 1021, 2098, 3059
PvuI CGATCG 2 cut(s) 1172, 2538
RsaI GTAC 6 cut(s) 776, 1411, 1852, 2052, 2686, 2826
RsaNI GTAC 6 cut(s) 775, 1410, 1851, 2051, 2685, 2825
RseI CAYNNNNRTG 3 cut(s) 1551, 1593, 1789
SacII CCGCGG 1 cut(s) 2761
SalI GTCGAC 2 cut(s) 1011, 1281
SapI GCTCTTC 1 cut(s) 795
SatI GCNGC 9 cut(s) 50, 103, 178, 250, 1221, 1224, 1396, 2358, 2601
Sau96I GGNCC 4 cut(s) 1952, 2117, 2937, 3052
ScaI AGTACT 1 cut(s) 2686
SchI GAGTC 3 cut(s) 130, 344, 563
ScrFI CCNGG 6 cut(s) 1004, 1406, 1642, 2798, 2853, 3090
SduI GDGCHC 2 cut(s) 611, 1352
SexAI ACCWGGT 1 cut(s) 1640
SfaNI GCATC 8 cut(s) 141, 226, 507, 1207, 1469, 1740, 2266, 2761
SfcI CTRYAG 1 cut(s) 1221
Sfr303I CCGCGG 1 cut(s) 2761
SfuI TTCGAA 1 cut(s) 1620
SgrBI CCGCGG 1 cut(s) 2761
SinI GGWCC 4 cut(s) 1952, 2117, 2937, 3052
SmiMI CAYNNNNRTG 3 cut(s) 1551, 1593, 1789
SmlI CTYRAG 3 cut(s) 404, 2680, 2807
SmoI CTYRAG 3 cut(s) 404, 2680, 2807
SpeI ACTAGT 3 cut(s) 1765, 2047, 2519
SsiI CCGC 9 cut(s) 249, 399, 657, 1095, 1878, 2279, 2460, 2758, 2760
SspI AATATT 1 cut(s) 1299
StyD4I CCNGG 6 cut(s) 1002, 1404, 1640, 2796, 2851, 3088
StyI CCWWGG 4 cut(s) 136, 531, 1750, 3055
TaiI ACGT 6 cut(s) 283, 905, 1789, 1863, 2041, 3035
TatI WGTACW 3 cut(s) 774, 2684, 2824
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 5 cut(s) 1247, 1617, 2258, 2294, 2329
TscAI CASTG 4 cut(s) 440, 835, 1473, 2936
TseFI GTSAC 6 cut(s) 12, 242, 665, 1336, 2590, 2675
TseI GCWGC 8 cut(s) 49, 102, 177, 1220, 1223, 1395, 2357, 2600
Tsp45I GTSAC 6 cut(s) 12, 242, 665, 1336, 2590, 2675
TspGWI ACGGA 1 cut(s) 2957
TspRI CASTG 4 cut(s) 440, 835, 1473, 2936
Vha464I CTTAAG 1 cut(s) 2680
VpaK11BI GGWCC 4 cut(s) 1952, 2117, 2937, 3052
VspI ATTAAT 1 cut(s) 1202
XagI CCTNNNNNAGG 1 cut(s) 535
XapI RAATTY 8 cut(s) 1267, 1498, 2391, 2493, 2738, 2790, 2835, 3014
XbaI TCTAGA 2 cut(s) 550, 2497
XceI RCATGY 3 cut(s) 188, 2173, 2598
XmiI GTMKAC 2 cut(s) 1012, 1282
XmnI GAANNNNTTC 1 cut(s) 2447
ZrmI AGTACT 1 cut(s) 2686
Zsp2I ATGCAT 1 cut(s) 1725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.